Rh5CG273900

Phytohormone-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0004227
Physical Location & Seq
Forward (+)
29638446 .. 29639343
898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG273900.1

Sequence Viewer

Length: 462 bp
ATGGCCAAAGAGACAAAGGCTCAAAGAACAGTTGGTGTAGGAGTTGGAGCCCTGTGGAGAGGTATGGCCAAGGATATAGTTTCTGTTATGCCAAAGATAATGCCTGATATAGTGCTCAGTGTCGAAGTGATTGAGGGAGATGGTGGCCTCGGTTCTGTTCTGCTCTTCAAACTTGGCAGTGATCCAAAATCCAAGAGACAACAGACGGAGAAGATTGTGGAACTAGATGAGTCTCAGTATCGATTTGCACTGCAAGTGTTGGAAGGCCCAGCACTGACACTGCGTGATTTTTCTTCATTGACAACCACGTTTCAGCTGAGTGCAATCAGTGAGAAGGAGACCCTGGTTGATATGAAGGTGGAATATAATACTGAAAAAGAAGAAACTAATATGGGAGAGATAGCAATGCAGCCAGTGCTTTCTTTCGTTCAACTCCTTGAGAAACTTGTATTAGAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

153

Amino Acids

16.89

Weight (kDa)

4.8

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 29 - 152 1.6e-10 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 176
AcoI YGGCCR 2 cut(s) 3, 66
AdeI CACNNNGTG 1 cut(s) 284
AgsI TTSAA 2 cut(s) 169, 431
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 1 cut(s) 316
AluI AGCT 1 cut(s) 316
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 4 cut(s) 5, 190, 237, 332
AlwI GGATC 1 cut(s) 176
AoxI GGCC 4 cut(s) 3, 66, 145, 265
ApeKI GCWGC 1 cut(s) 409
AspS9I GGNCC 1 cut(s) 266
BalI TGGCCA 2 cut(s) 5, 68
BanII GRGCYC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 421
BccI CCATC 1 cut(s) 134
BciT130I CCWGG 1 cut(s) 344
BcoDI GTCTC 4 cut(s) 5, 190, 237, 332
BfaI CTAG 1 cut(s) 224
BisI GCNGC 1 cut(s) 410
BlsI GCNGC 1 cut(s) 411
Bme1390I CCNGG 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 458
Bsa29I ATCGAT 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 3 cut(s) 69, 148, 342
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 344
BseCI ATCGAT 1 cut(s) 241
BseDI CCNNGG 3 cut(s) 69, 148, 342
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 3 cut(s) 130, 248, 308
BseNI ACTGG 1 cut(s) 413
BseXI GCAGC 1 cut(s) 421
BseYI CCCAGC 1 cut(s) 268
BshFI GGCC 4 cut(s) 5, 68, 147, 267
BshVI ATCGAT 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 117
BsmAI GTCTC 4 cut(s) 5, 190, 237, 332
BsnI GGCC 4 cut(s) 5, 68, 147, 267
Bso31I GGTCTC 1 cut(s) 332
Bsp1286I GDGCHC 2 cut(s) 52, 117
Bsp143I GATC 1 cut(s) 181
BspANI GGCC 4 cut(s) 5, 68, 147, 267
BspCNI CTCAG 3 cut(s) 129, 247, 309
BspDI ATCGAT 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 49
BspPI GGATC 1 cut(s) 176
BspQI GCTCTTC 1 cut(s) 170
BspTNI GGTCTC 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 1 cut(s) 413
BssECI CCNNGG 3 cut(s) 69, 148, 342
BssMI GATC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 415
BstDEI CTNAG 3 cut(s) 116, 234, 317
BstKTI GATC 1 cut(s) 184
BstMAI GTCTC 4 cut(s) 5, 190, 237, 332
BstMBI GATC 1 cut(s) 181
BstMWI GCNNNNNNNGC 1 cut(s) 415
BstNI CCWGG 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 342
BstV1I GCAGC 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 241
BsuRI GGCC 4 cut(s) 5, 68, 147, 267
BsuTUI ATCGAT 1 cut(s) 241
BtsI GCAGTG 3 cut(s) 184, 248, 278
BtsIMutI CAGTG 7 cut(s) 124, 184, 248, 272, 278, 334, 420
Cfr13I GGNCC 1 cut(s) 266
ClaI ATCGAT 1 cut(s) 241
CviJI RGCY 8 cut(s) 5, 20, 50, 68, 147, 267, 316, 412
CviKI_1 RGCY 8 cut(s) 5, 20, 50, 68, 147, 267, 316, 412
DdeI CTNAG 3 cut(s) 116, 234, 317
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
DraIII CACNNNGTG 1 cut(s) 284
EaeI YGGCCR 2 cut(s) 3, 66
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 69
FaiI YATR 8 cut(s) 65, 77, 89, 110, 353, 366, 392, 460
Fnu4HI GCNGC 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 1 cut(s) 410
FspBI CTAG 1 cut(s) 224
GluI GCNGC 1 cut(s) 410
GsaI CCCAGC 1 cut(s) 272
HaeIII GGCC 4 cut(s) 5, 68, 147, 267
HinfI GANTC 2 cut(s) 230, 455
HpyAV CCTTC 3 cut(s) 257, 328, 349
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 4 cut(s) 248, 253, 323, 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 415
HpyF3I CTNAG 3 cut(s) 116, 234, 317
HpySE526I ACGT 1 cut(s) 308
Kzo9I GATC 1 cut(s) 181
LguI GCTCTTC 1 cut(s) 170
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 6 cut(s) 65, 117, 282, 329, 356, 426
Lsp1109I GCAGC 1 cut(s) 421
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 308
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 4 cut(s) 157, 223, 285, 392
MhlI GDGCHC 2 cut(s) 52, 117
MlsI TGGCCA 2 cut(s) 5, 68
MluNI TGGCCA 2 cut(s) 5, 68
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 2 cut(s) 25, 240
MnlI CCTC 3 cut(s) 53, 127, 158
Mox20I TGGCCA 2 cut(s) 5, 68
MscI TGGCCA 2 cut(s) 5, 68
Msp20I TGGCCA 2 cut(s) 5, 68
MspA1I CMGCKG 1 cut(s) 316
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MwoI GCNNNNNNNGC 1 cut(s) 415
NdeII GATC 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 170
PfeI GAWTC 1 cut(s) 455
PkrI GCNGC 1 cut(s) 411
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 342
PspFI CCCAGC 1 cut(s) 268
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 49
PspPI GGNCC 1 cut(s) 266
PvuII CAGCTG 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 170
SatI GCNGC 1 cut(s) 410
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 2 cut(s) 52, 117
SetI ASST 4 cut(s) 64, 311, 318, 360
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 1 cut(s) 342
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 311
TaqI TCGA 2 cut(s) 123, 241
TfiI GAWTC 1 cut(s) 455
TscAI CASTG 7 cut(s) 124, 184, 255, 279, 285, 334, 420
TseI GCWGC 1 cut(s) 409
TspDTI ATGAA 2 cut(s) 285, 368
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 7 cut(s) 124, 184, 255, 279, 285, 334, 420
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.