Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0004227

Physical Location & Seq

Genomic Coordinates Forward (+)

13911880 .. 13926005

Gene Structure 14126 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0150100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3033 bp

ATGTCTCTCTCTCTGTCTTCCTCTCAGCTCTTCCTTTACACACCTCTCTTCTCTCTGCAGAGCTTTTCTCAGAAGCAAAGAGTATGTGGGTTTTGGAGAAAGCCACAGGCTTTGAGGAGTCTTGTGGTCAGAGCAGGCCCAAAGAAGATATCTTTTGGTAAAGAATGCAGAGAGGGATTGCAAATTGGGATAGATAAGCTTGCTGATGCTGTTTCTCTTACATTAGGACCTAGAGGACGTAATGTTGTTCTTTCTGAGTCTGAAAAAGTGAGAGTGATTAATGATGGTGTAACAATTGTTCAGTCCATAGAGCTAGCAGATACAATTGAGAACGCGGGAGCAATGCTAATCCAAGAGGTTGCAAGTAAAATGAATAATTTGGCCGGTGATGGTACTACCACTGCGATCATTTTGACACGAGAAATGATCAAAGGTGGATTATTGGCAGTGGCGTTTGGGGCTAACCCAATTTCTTTGAAGAAAGGAATGGAGAAGACTGTTAGTGAATTGGTCAAGGTCTTAAAGGAGAAAAGTCTTCCCGTCAAAGGAAGGAATGATATTAAAGCTGTGGCTTCAATTTCTGCCGGAAATGATGAATTCATAGGAAACTTGATTGCAGAAGCTATAGAAAAGATTGGTCCTGATGGAGTAATCTCAATTGAGTCGTCCTCATCATTTGAAACATCTGTTTTAGTCGAAGAAGGACTAAAGTTTGACAAGGGTTACATGTCCTCCCACTTCATTACTAATCAAGAAAGATCTATTGTGGAGTTTGACAAGGCTAAGGTCCTGGTAACTGATCAAAAGATTTCAACTGTTAAAGAAATTGTTCCTTTGCTGGAAAAGATAACCCAATTGAGTATCCCGCTGCTAATCATTGCAGAGGATATCTCAAGGCCAGTACTGGAGACACTAGTGGTGAATAAAATGCAGGGTTTACTTAATGTTGCTGTTGTTAAGTGCCCTGGATTTGGAGAGCGGAAGAAAGCTCTTTTGCAAGATATTGCACTTATGACAGGTGCTGATTTTCTCTCTGGAGATTTTGGTTTTACCCTTGACAGTGCAACATCAGATCAACTTGGAATTGCAAGAAAGGGTGCTTTCATTATTGCTCCTGAAAGTAAGAAGCTGGTGTTGTCATCTGCTGCCTCTAAATGGAGACAATTCAAGAGTAAGTTAACAAGTAAGTATGTATTACCTTATGCTGGGAGGAAAAAGAAACTGAAGAAACCACCAAAGATGTATGATTCCATCGAGAGAGATGAATGGAAACAGTTTGTTGCTTCACGATTATCAAATGATTGGCTGAAACTTCATATGGAGCAAAAAGAAAGGACAGCAAAGAGAAAGTATCACCACAGAATCTCAAGGAAGGGATATGTTGGATTAGAGGAAGAATTAATGGAGGCCATGTCTGAAGAAGAAGAACTTGATCGTTCTTATTTATGGAAGAAAGCACGGGAAGGTAAAAATGGAGAAATGGCAGAGGAGGTAGCTCCGGTGGCCAATGATATTGATAATTTGTTGGCTAAGAAAAATAAAGGCGAGCTCGCCATCTCGGGGTCTAATGATGTTCTAACAATGGCGCTAAAAACTCCGGAACATTCTGGTAGAGTAAGGGGTGTTGGGGGTAATGTTAACCCTTCTACTTACTTCCATCTTCCTAGACAGAGAAGGCAAAGCATTTCGAAGGCTATGTTTCTGGAGCATCAAAGGTTGTTTGAGGAGACAAAAAGAATGTTTGAAGCAGAACGTGAAAAAGAGCGAGCATATTTTGAGAAAAAGATTGCTGCATTGGAGGCAAAACTGAATGGCAATGTCCTTATTACACCTTCTCCTCCCATTTTTGATAAAGGAAGTAGCCATAGTAAAACTATGTTCAATGGTAATGATGCTTTAAAATCATCTCAATCTACCTATGATATTGAAATTGAGGAAGTGGAGGACATCGAGGTACCAAAAAAGCCAGGCAATATGAAGAATAAATCATGCAAGCTGGCAGCGGGTTCCATTCACAATATTGTGGCTTTAGGCACAATAATTGAAGAGAATGACTCTAATATTACTGTACATGGTATTCCTTTACAAGAGGGTCATTCGAGGGTTGCTGTTGATGTGTCCATTGATGCAAATGCTTTGCTTCCTGTCCCAATTCCAGATGAATTGGAGAATGTTGGTCAAGCTGTTGGGAGCTATGTGGCTTGGCCAAAGAAGCTCATTGTGTTGAATATGGAAAAGCAAAAAAAAAAGTATGTTGCTCCGGTAAAAGACACATTCATGAAACATGATGGTTCAAAAATGGTCCCTAGCAAATGTAAGGCACTGTATCAGATTGCAAAGATGACCATGGACAATAATTCAAAGGCACTGATGGAAACTCTAGAGGCTGAAATTTTCGGAGTGCCTCGAGATTTGTATATCTTCAGAGATGATATTATTCACTTAATGGAAATGCAATGGCTCAGCGCAAACTGCATAGTGGCTTACATGAGGTACCTATATGAGGTCCTTGAGAAATCTGACATGGTTGATACATTTGGGTTCACGGATCCTGCACGGGTCGTCTCTATTGGGGTATCAGATTCAAAGAATGTAGTGAACAATTTAGTACAACGGATGCAACATGGAAAGTCTGATCGTATATTTTTGGTGCCTTACAATCCCAGTAACCATTGGATGTTGACTATAATAAATGAAGAAAAAGACATTGTCTACATTGTGGACTCTTTGGAAAGACAATACCCCGAGTGGATGGAAACTGTGAACAATGCAATCAAATTATTTAATGGAATTACGGGTAGACATGGCAAAAAAGCACCACGATGGATAAATCTCTCGGGTGCACCAAAACAAGTGGGCGGTGGTGAATGTGGCTACTATGTCATGAGATACATGAAGGAACTGATTGAGGATCCAATGCTTTCTTTTGCACAGAAGTGGAGTAGGAAGTCGAAGAGAACCAGCTACTCACAAGAGCAAATTGATGAGGTGCGGATTGAATGGAGTGAGTTTGTTACAAGGAAATACTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bet_v_1

Protein Analysis

1010

Amino Acids

113.2

Weight (kDa)

8.94

Isoelectric Point (pI)

40.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	67 - 364	1.4e-39	TCP-1/cpn60 chaperonin family
DUF8039	PF26133	661 - 742	1.2e-14	Domain of unknown function (DUF8039)
Peptidase_C48	PF02902	880 - 991	3.7e-10	Ulp1 protease family, C-terminal catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004227)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g20810 FvH4_3g20810 FvH4_3g20820
malus_domestica	MD03G1220400.v1.1 MD03G1220500.v1.1 MD11G1238400.v1.1
prunus_persica	Prupe.4G188200_v2.0.a1 Prupe.4G188300_v2.0.a1 Prupe.4G188400_v2.0.a1
pyrus_communis	pycom11g21000
rosa_chinensis	RchiOBHm_Chr5g0035101 RchiOBHm_Chr5g0035111
rosa_laevigata	RLG00000033596 RLG00000033597 RLG00000033598
rosa_multiflora	Rmu_co8030986.1_g000001 Rmu_sc0000879.1_g000009 Rmu_sc0000879.1_g000011 Rmu_sc0005362.1_g000006
rosa_roxburghii	Rroxscaffold_1G00045280 Rroxscaffold_1G00045300
rosa_rugosa	Rorug05G0149900 Rorug05G0150000 Rorug05G0150100
rosa_samantha	Rh5AG242900 Rh5BG242400 Rh5BG242500 Rh5CG273900 Rh5CG274000 Rh5CG274100 Rh5DG251000 Rh5DG251100
rosa_wichuraiana	Rw5G022200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	1954, 2493
AccB1I	GGYRCC	3 cut(s)	1954, 2493, 2650
AccBSI	CCGCTC	1 cut(s)	979
AccI	GTMKAC	2 cut(s)	2712, 2800
AccII	CGCG	1 cut(s)	335
AccIII	TCCGGA	1 cut(s)	1597
AciI	CCGC	6 cut(s)	335, 866, 979, 2003, 2859, 2992
AclWI	GGATC	4 cut(s)	2543, 2556, 2906, 2919
AcoI	YGGCCR	3 cut(s)	381, 1503, 2204
AcsI	RAATTY	2 cut(s)	596, 2391
AcuI	CTGAAG	3 cut(s)	1244, 1437, 2407
AfaI	GTAC	6 cut(s)	394, 903, 1956, 2070, 2495, 2610
AfiI	CCNNNNNNNGG	6 cut(s)	545, 971, 1155, 1205, 1560, 2503
AflIII	ACRYGT	1 cut(s)	726
AhlI	ACTAGT	1 cut(s)	913
AjnI	CCWGG	3 cut(s)	789, 964, 1966
AleI	CACNNNNGTG	1 cut(s)	2935
Alw21I	GWGCWC	2 cut(s)	1551, 2845
Alw26I	GTCTC	5 cut(s)	9, 902, 1153, 1721, 2569
Alw44I	GTGCAC	1 cut(s)	2841
AlwI	GGATC	4 cut(s)	2543, 2556, 2906, 2919
AlwNI	CAGNNNCTG	1 cut(s)	1022
Ama87I	CYCGRG	4 cut(s)	1558, 2406, 2744, 2836
Aor13HI	TCCGGA	1 cut(s)	1597
AoxI	GGCC	6 cut(s)	136, 381, 896, 1407, 1503, 2204
ApaLI	GTGCAC	1 cut(s)	2841
ApeKI	GCWGC	4 cut(s)	868, 1145, 1790, 2000
ApoI	RAATTY	2 cut(s)	596, 2391
AseI	ATTAAT	2 cut(s)	279, 1400
Asp700I	GAANNNNTTC	1 cut(s)	828
Asp718I	GGTACC	2 cut(s)	1954, 2493
AspLEI	GCGC	2 cut(s)	1588, 2468
AspS9I	GGNCC	6 cut(s)	137, 227, 638, 787, 2302, 2506
AsuHPI	GGTGA	4 cut(s)	398, 931, 1346, 2876
AsuII	TTCGAA	1 cut(s)	1688
AsuNHI	GCTAGC	1 cut(s)	313
AvaI	CYCGRG	4 cut(s)	1558, 2406, 2744, 2836
AvaII	GGWCC	5 cut(s)	227, 638, 787, 2302, 2506
BaeGI	GKGCMC	2 cut(s)	965, 2845
BalI	TGGCCA	2 cut(s)	1505, 2206
BamHI	GGATCC	2 cut(s)	2548, 2911
BanI	GGYRCC	3 cut(s)	1954, 2493, 2650
BanII	GRGCYC	1 cut(s)	1551
BauI	CACGAG	1 cut(s)	417
BbsI	GAAGAC	3 cut(s)	9, 500, 527
Bbv12I	GWGCWC	2 cut(s)	1551, 2845
BbvI	GCAGC	4 cut(s)	855, 1132, 1777, 2012
BciT130I	CCWGG	3 cut(s)	791, 966, 1968
BciVI	GTATCC	1 cut(s)	872
BclI	TGATCA	2 cut(s)	426, 799
BcoDI	GTCTC	5 cut(s)	9, 902, 1153, 1721, 2569
BcuI	ACTAGT	1 cut(s)	913
BfaI	CTAG	6 cut(s)	231, 314, 914, 1665, 2307, 2381
BfmI	CTRYAG	2 cut(s)	56, 624
BfoI	RGCGCY	1 cut(s)	1589
BfuI	GTATCC	1 cut(s)	872
BglII	AGATCT	1 cut(s)	758
BisI	GCNGC	4 cut(s)	869, 1146, 1791, 2001
BlpI	GCTNAGC	1 cut(s)	2462
BlsI	GCNGC	4 cut(s)	870, 1147, 1792, 2002
BmcAI	AGTACT	1 cut(s)	903
Bme1390I	CCNGG	3 cut(s)	791, 966, 1968
Bme18I	GGWCC	5 cut(s)	227, 638, 787, 2302, 2506
BmeT110I	CYCGRG	4 cut(s)	1558, 2406, 2744, 2836
BmgT120I	GGNCC	6 cut(s)	137, 227, 638, 787, 2302, 2506
BmiI	GGNNCC	7 cut(s)	1956, 2008, 2304, 2495, 2550, 2652, 2913
BmrFI	CCNGG	3 cut(s)	791, 966, 1968
BmrI	ACTGGG	1 cut(s)	2658
BmsI	GCATC	5 cut(s)	196, 1717, 1882, 2116, 2607
BmtI	GCTAGC	1 cut(s)	317
BmuI	ACTGGG	1 cut(s)	2658
BpiI	GAAGAC	3 cut(s)	9, 500, 527
BplI	GAGNNNNNCTC	8 cut(s)	52, 84, 653, 685, 875, 907, 2039, 2071
BpmI	CTGGAG	3 cut(s)	926, 1056, 1724
Bpu10I	CCTNAGC	1 cut(s)	783
Bpu1102I	GCTNAGC	1 cut(s)	2462
Bpu14I	TTCGAA	1 cut(s)	1688
BpuEI	CTTGAG	3 cut(s)	877, 1351, 2531
BsaJI	CCNNGG	2 cut(s)	964, 2346
BsaWI	WCCGGW	3 cut(s)	1498, 1597, 2260
BsaXI	ACNNNNNCTCC	6 cut(s)	716, 746, 1397, 1427, 1819, 1849
Bsc4I	CCNNNNNNNGG	6 cut(s)	545, 971, 1155, 1205, 1560, 2503
Bse118I	RCCGGY	1 cut(s)	383
Bse1I	ACTGG	3 cut(s)	899, 909, 2664
Bse3DI	GCAATG	4 cut(s)	348, 876, 1822, 2462
BseAI	TCCGGA	1 cut(s)	1597
BseBI	CCWGG	3 cut(s)	791, 966, 1968
BseDI	CCNNGG	2 cut(s)	964, 2346
BseGI	GGATG	3 cut(s)	2622, 2682, 2757
BseLI	CCNNNNNNNGG	6 cut(s)	545, 971, 1155, 1205, 1560, 2503
BseMI	GCAATG	4 cut(s)	348, 876, 1822, 2462
BseMII	CTCAG	4 cut(s)	38, 83, 246, 2476
BseNI	ACTGG	3 cut(s)	899, 909, 2664
BseRI	GAGGAG	4 cut(s)	130, 1502, 1739, 1827
BseSI	GKGCMC	2 cut(s)	965, 2845
BseXI	GCAGC	4 cut(s)	855, 1132, 1777, 2012
BseYI	CCCAGC	1 cut(s)	1205
BsgI	GTGCAG	1 cut(s)	2538
Bsh1236I	CGCG	1 cut(s)	335
BshFI	GGCC	6 cut(s)	138, 383, 898, 1409, 1505, 2206
BshNI	GGYRCC	3 cut(s)	1954, 2493, 2650
BsiHKAI	GWGCWC	2 cut(s)	1551, 2845
BsiHKCI	CYCGRG	4 cut(s)	1558, 2406, 2744, 2836
BsiSI	CCGG	5 cut(s)	384, 585, 1499, 1598, 2261
BslFI	GGGAC	2 cut(s)	2132, 2288
BslI	CCNNNNNNNGG	6 cut(s)	545, 971, 1155, 1205, 1560, 2503
BsmAI	GTCTC	5 cut(s)	9, 902, 1153, 1721, 2569
BsmBI	CGTCTC	1 cut(s)	2569
BsmFI	GGGAC	2 cut(s)	2132, 2288
BsmI	GAATGC	1 cut(s)	170
BsnI	GGCC	6 cut(s)	138, 383, 898, 1409, 1505, 2206
BsoBI	CYCGRG	4 cut(s)	1558, 2406, 2744, 2836
Bsp119I	TTCGAA	1 cut(s)	1688
Bsp1286I	GDGCHC	3 cut(s)	965, 1551, 2845
Bsp13I	TCCGGA	1 cut(s)	1597
Bsp1407I	TGTACA	1 cut(s)	2068
Bsp143I	GATC	9 cut(s)	405, 426, 758, 799, 1072, 1432, 2548, 2635, 2911
Bsp1720I	GCTNAGC	1 cut(s)	2462
Bsp19I	CCATGG	1 cut(s)	2346
BspACI	CCGC	6 cut(s)	335, 866, 979, 2003, 2859, 2992
BspANI	GGCC	6 cut(s)	138, 383, 898, 1409, 1505, 2206
BspCNI	CTCAG	4 cut(s)	37, 82, 247, 2475
BspEI	TCCGGA	1 cut(s)	1597
BspFNI	CGCG	1 cut(s)	335
BspHI	TCATGA	2 cut(s)	2277, 2883
BspLI	GGNNCC	7 cut(s)	1956, 2008, 2304, 2495, 2550, 2652, 2913
BspMAI	CTGCAG	1 cut(s)	60
BspOI	GCTAGC	1 cut(s)	317
BspPI	GGATC	4 cut(s)	2543, 2556, 2906, 2919
BspQI	GCTCTTC	1 cut(s)	35
BspT104I	TTCGAA	1 cut(s)	1688
BspT107I	GGYRCC	3 cut(s)	1954, 2493, 2650
BsrBI	CCGCTC	1 cut(s)	979
BsrDI	GCAATG	4 cut(s)	348, 876, 1822, 2462
BsrFI	RCCGGY	1 cut(s)	383
BsrGI	TGTACA	1 cut(s)	2068
BsrI	ACTGG	3 cut(s)	899, 909, 2664
BssAI	RCCGGY	1 cut(s)	383
BssECI	CCNNGG	2 cut(s)	964, 2346
BssMI	GATC	9 cut(s)	405, 426, 758, 799, 1072, 1432, 2548, 2635, 2911
BssSI	CACGAG	1 cut(s)	417
BssT1I	CCWWGG	1 cut(s)	2346
Bst2BI	CACGAG	1 cut(s)	417
Bst2UI	CCWGG	3 cut(s)	791, 966, 1968
Bst4CI	ACNGT	7 cut(s)	499, 817, 1061, 1275, 2068, 2325, 2761
Bst6I	CTCTTC	4 cut(s)	35, 53, 2040, 2948
BstAUI	TGTACA	1 cut(s)	2068
BstBI	TTCGAA	1 cut(s)	1688
BstC8I	GCNNGC	8 cut(s)	136, 201, 315, 1547, 1551, 1767, 1994, 1998
BstDEI	CTNAG	6 cut(s)	24, 69, 255, 783, 1530, 2462
BstDSI	CCRYGG	1 cut(s)	2346
BstENI	CCTNNNNNAGG	1 cut(s)	2501
BstF5I	GGATG	3 cut(s)	2622, 2682, 2757
BstFNI	CGCG	1 cut(s)	335
BstH2I	RGCGCY	1 cut(s)	1589
BstHHI	GCGC	2 cut(s)	1588, 2468
BstKTI	GATC	9 cut(s)	408, 429, 761, 802, 1075, 1435, 2551, 2638, 2914
BstMAI	GTCTC	5 cut(s)	9, 902, 1153, 1721, 2569
BstMBI	GATC	9 cut(s)	405, 426, 758, 799, 1072, 1432, 2548, 2635, 2911
BstMWI	GCNNNNNNNGC	5 cut(s)	458, 1502, 1799, 2212, 2472
BstNI	CCWGG	3 cut(s)	791, 966, 1968
BstNSI	RCATGY	1 cut(s)	730
BstSCI	CCNGG	3 cut(s)	789, 964, 1966
BstSFI	CTRYAG	2 cut(s)	56, 624
BstSLI	GKGCMC	2 cut(s)	965, 2845
BstUI	CGCG	1 cut(s)	335
BstV1I	GCAGC	4 cut(s)	855, 1132, 1777, 2012
BstV2I	GAAGAC	3 cut(s)	9, 500, 527
BstX2I	RGATCY	3 cut(s)	758, 2548, 2911
BstYI	RGATCY	3 cut(s)	758, 2548, 2911
BsuI	GTATCC	1 cut(s)	872
BsuRI	GGCC	6 cut(s)	138, 383, 898, 1409, 1505, 2206
BtgI	CCRYGG	1 cut(s)	2346
BtsCI	GGATG	3 cut(s)	2622, 2682, 2757
BtsI	GCAGTG	2 cut(s)	399, 453
BtsIMutI	CAGTG	5 cut(s)	399, 453, 1066, 2321, 2366
Cac8I	GCNNGC	8 cut(s)	136, 201, 315, 1547, 1551, 1767, 1994, 1998
CaiI	CAGNNNCTG	1 cut(s)	1022
CciI	TCATGA	2 cut(s)	2277, 2883
CfoI	GCGC	2 cut(s)	1588, 2468
Cfr10I	RCCGGY	1 cut(s)	383
Cfr13I	GGNCC	6 cut(s)	137, 227, 638, 787, 2302, 2506
Csp6I	GTAC	6 cut(s)	393, 902, 1955, 2069, 2494, 2609
CspCI	CAANNNNNGTGG	4 cut(s)	93, 128, 2835, 2870
CviQI	GTAC	6 cut(s)	393, 902, 1955, 2069, 2494, 2609
DdeI	CTNAG	6 cut(s)	24, 69, 255, 783, 1530, 2462
DpnI	GATC	9 cut(s)	407, 428, 760, 801, 1074, 1434, 2550, 2637, 2913
DpnII	GATC	9 cut(s)	405, 426, 758, 799, 1072, 1432, 2548, 2635, 2911
DraI	TTTAAA	1 cut(s)	1899
EaeI	YGGCCR	3 cut(s)	381, 1503, 2204
Eam1104I	CTCTTC	4 cut(s)	35, 53, 2040, 2948
EarI	CTCTTC	4 cut(s)	35, 53, 2040, 2948
Ecl136II	GAGCTC	1 cut(s)	1549
Eco130I	CCWWGG	1 cut(s)	2346
Eco24I	GRGCYC	1 cut(s)	1551
Eco32I	GATATC	2 cut(s)	150, 889
Eco47I	GGWCC	5 cut(s)	227, 638, 787, 2302, 2506
Eco53kI	GAGCTC	1 cut(s)	1549
Eco57I	CTGAAG	3 cut(s)	1244, 1437, 2407
Eco88I	CYCGRG	4 cut(s)	1558, 2406, 2744, 2836
EcoICRI	GAGCTC	1 cut(s)	1549
EcoNI	CCTNNNNNAGG	1 cut(s)	2501
EcoO109I	RGGNCCY	3 cut(s)	227, 787, 2506
EcoRI	GAATTC	1 cut(s)	596
EcoRII	CCWGG	3 cut(s)	789, 964, 1966
EcoRV	GATATC	2 cut(s)	150, 889
EcoT14I	CCWWGG	1 cut(s)	2346
EcoT38I	GRGCYC	1 cut(s)	1551
ErhI	CCWWGG	1 cut(s)	2346
Esp3I	CGTCTC	1 cut(s)	2569
FalI	AAGNNNNNCTT	2 cut(s)	1413, 1445
FaqI	GGGAC	2 cut(s)	2132, 2288
FauI	CCCGC	3 cut(s)	328, 873, 1996
FauNDI	CATATG	1 cut(s)	1317
FbaI	TGATCA	2 cut(s)	426, 799
FblI	GTMKAC	2 cut(s)	2712, 2800
Fnu4HI	GCNGC	4 cut(s)	869, 1146, 1791, 2001
FokI	GGATG	3 cut(s)	2629, 2689, 2764
FriOI	GRGCYC	1 cut(s)	1551
Fsp4HI	GCNGC	4 cut(s)	869, 1146, 1791, 2001
FspBI	CTAG	6 cut(s)	231, 314, 914, 1665, 2307, 2381
GlaI	GCGC	2 cut(s)	1587, 2467
GluI	GCNGC	4 cut(s)	869, 1146, 1791, 2001
GsaI	CCCAGC	1 cut(s)	1209
GsuI	CTGGAG	3 cut(s)	926, 1056, 1724
HaeII	RGCGCY	1 cut(s)	1589
HaeIII	GGCC	6 cut(s)	138, 383, 898, 1409, 1505, 2206
HapII	CCGG	5 cut(s)	384, 585, 1499, 1598, 2261
HhaI	GCGC	2 cut(s)	1588, 2468
Hin6I	GCGC	2 cut(s)	1586, 2466
HinP1I	GCGC	2 cut(s)	1586, 2466
HincII	GTYRAC	3 cut(s)	1179, 1639, 2682
HindII	GTYRAC	3 cut(s)	1179, 1639, 2682
HindIII	AAGCTT	1 cut(s)	197
HinfI	GANTC	8 cut(s)	118, 257, 662, 1247, 1362, 2054, 2582, 2723
HpaI	GTTAAC	2 cut(s)	1179, 1639
HpaII	CCGG	5 cut(s)	384, 585, 1499, 1598, 2261
HphI	GGTGA	4 cut(s)	398, 931, 1346, 2876
HpyAV	CCTTC	9 cut(s)	543, 695, 1366, 1457, 1653, 1668, 1684, 1842, 2890
HpyCH4III	ACNGT	7 cut(s)	499, 817, 1061, 1275, 2068, 2325, 2761
HpyCH4IV	ACGT	2 cut(s)	238, 1753
HpyF10VI	GCNNNNNNNGC	5 cut(s)	458, 1502, 1799, 2212, 2472
HpyF3I	CTNAG	6 cut(s)	24, 69, 255, 783, 1530, 2462
HpySE526I	ACGT	2 cut(s)	238, 1753
HspAI	GCGC	2 cut(s)	1586, 2466
Kpn2I	TCCGGA	1 cut(s)	1597
KpnI	GGTACC	2 cut(s)	1958, 2497
Ksp22I	TGATCA	2 cut(s)	426, 799
KspAI	GTTAAC	2 cut(s)	1179, 1639
Kzo9I	GATC	9 cut(s)	405, 426, 758, 799, 1072, 1432, 2548, 2635, 2911
LguI	GCTCTTC	1 cut(s)	35
LmnI	GCTCC	7 cut(s)	338, 1117, 1321, 1501, 1705, 2190, 2263
Lsp1109I	GCAGC	4 cut(s)	855, 1132, 1777, 2012
LweI	GCATC	5 cut(s)	196, 1717, 1882, 2116, 2607
MaeI	CTAG	6 cut(s)	231, 314, 914, 1665, 2307, 2381
MaeII	ACGT	2 cut(s)	238, 1753
MaeIII	GTNAC	5 cut(s)	289, 722, 793, 2666, 3013
MalI	GATC	9 cut(s)	407, 428, 760, 801, 1074, 1434, 2550, 2637, 2913
MbiI	CCGCTC	1 cut(s)	979
MboI	GATC	9 cut(s)	405, 426, 758, 799, 1072, 1432, 2548, 2635, 2911
MfeI	CAATTG	4 cut(s)	294, 324, 657, 854
MflI	RGATCY	3 cut(s)	758, 2548, 2911
MhlI	GDGCHC	3 cut(s)	965, 1551, 2845
MlsI	TGGCCA	2 cut(s)	1505, 2206
MluNI	TGGCCA	2 cut(s)	1505, 2206
MlyI	GAGTC	5 cut(s)	127, 266, 671, 2048, 2717
MmeI	TCCRAC	1 cut(s)	1363
Mox20I	TGGCCA	2 cut(s)	1505, 2206
MroI	TCCGGA	1 cut(s)	1597
MroXI	GAANNNNTTC	1 cut(s)	828
MscI	TGGCCA	2 cut(s)	1505, 2206
MslI	CAYNNNNRTG	3 cut(s)	2276, 2821, 2935
Msp20I	TGGCCA	2 cut(s)	1505, 2206
MspA1I	CMGCKG	2 cut(s)	868, 2003
MspI	CCGG	5 cut(s)	384, 585, 1499, 1598, 2261
MspR9I	CCNGG	3 cut(s)	791, 966, 1968
MunI	CAATTG	4 cut(s)	294, 324, 657, 854
Mva1269I	GAATGC	1 cut(s)	170
MvaI	CCWGG	3 cut(s)	791, 966, 1968
MvnI	CGCG	1 cut(s)	335
MwoI	GCNNNNNNNGC	5 cut(s)	458, 1502, 1799, 2212, 2472
NcoI	CCATGG	1 cut(s)	2346
NdeI	CATATG	1 cut(s)	1317
NdeII	GATC	9 cut(s)	405, 426, 758, 799, 1072, 1432, 2548, 2635, 2911
NheI	GCTAGC	1 cut(s)	313
NlaIV	GGNNCC	7 cut(s)	1956, 2008, 2304, 2495, 2550, 2652, 2913
NspI	RCATGY	1 cut(s)	730
NspV	TTCGAA	1 cut(s)	1688
OliI	CACNNNNGTG	1 cut(s)	2935
PaeR7I	CTCGAG	1 cut(s)	2406
PagI	TCATGA	2 cut(s)	2277, 2883
PciI	ACATGT	1 cut(s)	726
PciSI	GCTCTTC	1 cut(s)	35
PctI	GAATGC	1 cut(s)	170
PdmI	GAANNNNTTC	1 cut(s)	828
PfeI	GAWTC	3 cut(s)	1247, 1362, 2582
PflFI	GACNNNGTC	1 cut(s)	2708
PkrI	GCNGC	4 cut(s)	870, 1147, 1792, 2002
PleI	GAGTC	5 cut(s)	126, 265, 670, 2048, 2717
PpsI	GAGTC	5 cut(s)	126, 265, 670, 2048, 2717
PpuMI	RGGWCCY	3 cut(s)	227, 787, 2506
PscI	ACATGT	1 cut(s)	726
PshBI	ATTAAT	2 cut(s)	279, 1400
Psp124BI	GAGCTC	1 cut(s)	1551
Psp5II	RGGWCCY	3 cut(s)	227, 787, 2506
Psp6I	CCWGG	3 cut(s)	789, 964, 1966
PspFI	CCCAGC	1 cut(s)	1205
PspGI	CCWGG	3 cut(s)	789, 964, 1966
PspN4I	GGNNCC	7 cut(s)	1956, 2008, 2304, 2495, 2550, 2652, 2913
PspPI	GGNCC	6 cut(s)	137, 227, 638, 787, 2302, 2506
PspPPI	RGGWCCY	3 cut(s)	227, 787, 2506
PsrI	GAACNNNNNNTAC	2 cut(s)	282, 314
PstI	CTGCAG	1 cut(s)	60
PstNI	CAGNNNCTG	1 cut(s)	1022
PsuI	RGATCY	3 cut(s)	758, 2548, 2911
PsyI	GACNNNGTC	1 cut(s)	2708
RsaI	GTAC	6 cut(s)	394, 903, 1956, 2070, 2495, 2610
RsaNI	GTAC	6 cut(s)	393, 902, 1955, 2069, 2494, 2609
RseI	CAYNNNNRTG	3 cut(s)	2276, 2821, 2935
SacI	GAGCTC	1 cut(s)	1551
SapI	GCTCTTC	1 cut(s)	35
SatI	GCNGC	4 cut(s)	869, 1146, 1791, 2001
Sau3AI	GATC	9 cut(s)	405, 426, 758, 799, 1072, 1432, 2548, 2635, 2911
Sau96I	GGNCC	6 cut(s)	137, 227, 638, 787, 2302, 2506
ScaI	AGTACT	1 cut(s)	903
SchI	GAGTC	5 cut(s)	127, 266, 671, 2048, 2717
ScrFI	CCNGG	3 cut(s)	791, 966, 1968
SduI	GDGCHC	3 cut(s)	965, 1551, 2845
SfaNI	GCATC	5 cut(s)	196, 1717, 1882, 2116, 2607
SfcI	CTRYAG	2 cut(s)	56, 624
Sfr274I	CTCGAG	1 cut(s)	2406
SfuI	TTCGAA	1 cut(s)	1688
SinI	GGWCC	5 cut(s)	227, 638, 787, 2302, 2506
SlaI	CTCGAG	1 cut(s)	2406
SmiMI	CAYNNNNRTG	3 cut(s)	2276, 2821, 2935
SmlI	CTYRAG	4 cut(s)	892, 1366, 2406, 2510
SmoI	CTYRAG	4 cut(s)	892, 1366, 2406, 2510
SpeI	ACTAGT	1 cut(s)	913
SsiI	CCGC	6 cut(s)	335, 866, 979, 2003, 2859, 2992
SspI	AATATT	2 cut(s)	2020, 2062
SspMI	CTAG	6 cut(s)	231, 314, 914, 1665, 2307, 2381
SstI	GAGCTC	1 cut(s)	1551
StyD4I	CCNGG	3 cut(s)	789, 964, 1966
StyI	CCWWGG	1 cut(s)	2346
TaaI	ACNGT	7 cut(s)	499, 817, 1061, 1275, 2068, 2325, 2761
TaiI	ACGT	2 cut(s)	241, 1756
TaqI	TCGA	7 cut(s)	696, 1254, 1688, 1950, 2099, 2407, 2951
TatI	WGTACW	3 cut(s)	901, 2068, 2608
TfiI	GAWTC	3 cut(s)	1247, 1362, 2582
TscAI	CASTG	5 cut(s)	406, 453, 1066, 2328, 2373
TseI	GCWGC	4 cut(s)	868, 1145, 1790, 2000
TspGWI	ACGGA	2 cut(s)	2561, 2629
TspRI	CASTG	5 cut(s)	406, 453, 1066, 2328, 2373
Tth111I	GACNNNGTC	1 cut(s)	2708
VneI	GTGCAC	1 cut(s)	2841
VpaK11BI	GGWCC	5 cut(s)	227, 638, 787, 2302, 2506
VspI	ATTAAT	2 cut(s)	279, 1400
XagI	CCTNNNNNAGG	1 cut(s)	2501
XapI	RAATTY	2 cut(s)	596, 2391
XbaI	TCTAGA	1 cut(s)	2380
XceI	RCATGY	1 cut(s)	730
XhoI	CTCGAG	1 cut(s)	2406
XmiI	GTMKAC	2 cut(s)	2712, 2800
XmnI	GAANNNNTTC	1 cut(s)	828
XspI	CTAG	6 cut(s)	231, 314, 914, 1665, 2307, 2381
ZrmI	AGTACT	1 cut(s)	903

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.