FvH4_3g23850

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001913
Physical Location & Seq
Reverse (-)
16923096 .. 16925525
2430 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23850.t1

Sequence Viewer

Length: 678 bp
ATGGGTATCTCTCGAGATTCCATGCACAAGAGACGTGCCACTGGAGGCAAGAAGAAGGCCTGGAGGAAGAAGAGAAAGTACGAGCTCGGAAGGCAGCCAGCCAACACTAAACTGTCCAGCAACAAGACAGTGAGGAGGATTAGGGTTCGAGGTGGAAATGTGAAGTGGCGTGCTTTGAGGCTTGACACAGGAAATTTCTCTTGGGGTAGTGAGGCTGTGACCAGGAAGACCCGTCTTCTTGATGTTGTTTACAATGCATCTAACAATGAGCTTGTGCGTACTCAAACCTTGGTGAAGAGTGCCATTGTTCAGGTTGATGCAGCACCTTTCAAGCAGTGGTATTCCACCCACTATGGTGTTGAAATTGGCCGCAAGAAGAAAACCGCTGCTGCTATCAAGAAAGAAGCTGAGGATGGTGAGGCCGCTACCGCTGCTGCTGCAGCTGCTGAGGAGGTTAAGAAGAGCAAGCATGTAGAAAGGAAGTTGGAGAAGCGCCAGCAGACTCGCGTGCTTGACCACCACATTGAGGAACAATTTGGTGGCGGTCGTCTATTGGCTTGCATCTCTTCACGACCTGGTCAATGCGGCCGTGCAGATGGATACATCTTGGAGGGCAAAGAGCTAGAGTTTTACATGAAGAAGCTCCAGAGGAAGAAGGGAAAGGGAGCAACTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000313 GO:0000462 GO:0000956 GO:0002164 GO:0002181 GO:0002182 GO:0002376 GO:0002520 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0005840 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006414 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009547 GO:0009570 GO:0009790 GO:0009791 GO:0009792 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0012505 GO:0015031 GO:0015833 GO:0015935 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0019222 GO:0019439 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0030097 GO:0030490 GO:0030684 GO:0030686 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0034655 GO:0034660 GO:0035166 GO:0035167 GO:0042254 GO:0042255 GO:0042274 GO:0042886 GO:0043009 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043253 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048513 GO:0048519 GO:0048534 GO:0048542 GO:0048569 GO:0048731 GO:0048732 GO:0048856 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0090304 GO:1901360 GO:1901361 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N Ribosomal_S8e

Protein Analysis

226

Amino Acids

25.31

Weight (kDa)

10.43

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8e PF01201 5 - 203 8.4e-53 Ribosomal protein S8e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 507
AciI CCGC 6 cut(s) 370, 384, 423, 429, 543, 585
AcoI YGGCCR 2 cut(s) 367, 586
AcsI RAATTY 1 cut(s) 193
AfaI GTAC 2 cut(s) 80, 280
AfiI CCNNNNNNNGG 1 cut(s) 526
AgsI TTSAA 2 cut(s) 331, 362
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 3 cut(s) 59, 221, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 184, 216
AleI CACNNNNGTG 1 cut(s) 354
AluBI AGCT 6 cut(s) 85, 271, 407, 443, 622, 643
AluI AGCT 6 cut(s) 85, 271, 407, 443, 622, 643
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 25
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 12
AoxI GGCC 4 cut(s) 57, 367, 420, 586
ApeKI GCWGC 9 cut(s) 94, 320, 386, 389, 431, 434, 437, 440, 443
ApoI RAATTY 1 cut(s) 193
AspLEI GCGC 1 cut(s) 495
AsuHPI GGTGA 2 cut(s) 304, 428
AvaI CYCGRG 1 cut(s) 12
BanII GRGCYC 1 cut(s) 87
BarI GAAGNNNNNNTAC 2 cut(s) 62, 94
BbsI GAAGAC 2 cut(s) 227, 233
Bbv12I GWGCWC 1 cut(s) 87
BbvCI CCTCAGC 2 cut(s) 408, 447
BbvI GCAGC 9 cut(s) 106, 332, 373, 376, 418, 421, 424, 430, 452
BccI CCATC 2 cut(s) 407, 590
BceAI ACGGC 1 cut(s) 573
BciT130I CCWGG 3 cut(s) 61, 223, 576
BciVI GTATCC 1 cut(s) 593
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 2 cut(s) 623, 676
BfmI CTRYAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 496
BfuI GTATCC 1 cut(s) 593
Bme1390I CCNGG 3 cut(s) 61, 223, 576
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 35
BmrFI CCNGG 3 cut(s) 61, 223, 576
BmsI GCATC 3 cut(s) 266, 307, 570
BpiI GAAGAC 2 cut(s) 227, 233
BpmI CTGGAG 3 cut(s) 63, 82, 629
Bpu10I CCTNAGC 2 cut(s) 408, 447
BsaJI CCNNGG 1 cut(s) 288
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 46
BseBI CCWGG 3 cut(s) 61, 223, 576
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMII CTCAG 2 cut(s) 399, 438
BseNI ACTGG 1 cut(s) 46
BseRI GAGGAG 2 cut(s) 148, 464
BseX3I CGGCCG 1 cut(s) 586
BseXI GCAGC 9 cut(s) 106, 332, 373, 376, 418, 421, 424, 430, 452
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 2 cut(s) 547, 589
BshFI GGCC 4 cut(s) 59, 369, 422, 588
BsiEI CGRYCG 2 cut(s) 547, 589
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 526
BsmAI GTCTC 1 cut(s) 25
BsmBI CGTCTC 1 cut(s) 25
BsnI GGCC 4 cut(s) 59, 369, 422, 588
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 87
BspACI CCGC 6 cut(s) 370, 384, 423, 429, 543, 585
BspANI GGCC 4 cut(s) 59, 369, 422, 588
BspCNI CTCAG 2 cut(s) 400, 439
BspFNI CGCG 1 cut(s) 507
BspMAI CTGCAG 1 cut(s) 442
BspQI GCTCTTC 1 cut(s) 455
BsrI ACTGG 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 3 cut(s) 61, 223, 576
Bst4CI ACNGT 2 cut(s) 114, 130
Bst6I CTCTTC 4 cut(s) 65, 290, 455, 571
BstC8I GCNNGC 6 cut(s) 99, 171, 467, 497, 509, 559
BstDEI CTNAG 2 cut(s) 408, 447
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 1 cut(s) 507
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 1 cut(s) 495
BstMAI GTCTC 1 cut(s) 25
BstMCI CGRYCG 2 cut(s) 547, 589
BstMWI GCNNNNNNNGC 6 cut(s) 91, 428, 431, 437, 440, 443
BstNI CCWGG 3 cut(s) 61, 223, 576
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 3 cut(s) 59, 221, 574
BstSFI CTRYAG 1 cut(s) 438
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 9 cut(s) 106, 332, 373, 376, 418, 421, 424, 430, 452
BstV2I GAAGAC 2 cut(s) 227, 233
BstZI CGGCCG 1 cut(s) 586
BsuI GTATCC 1 cut(s) 593
BsuRI GGCC 4 cut(s) 59, 369, 422, 588
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 3 cut(s) 39, 135, 341
Cac8I GCNNGC 6 cut(s) 99, 171, 467, 497, 509, 559
CaiI CAGNNNCTG 1 cut(s) 446
CfoI GCGC 1 cut(s) 495
CsiI ACCWGGT 1 cut(s) 574
Csp6I GTAC 2 cut(s) 79, 279
CviAII CATG 3 cut(s) 22, 470, 634
CviQI GTAC 2 cut(s) 79, 279
DdeI CTNAG 2 cut(s) 408, 447
EaeI YGGCCR 2 cut(s) 367, 586
EagI CGGCCG 1 cut(s) 586
Eam1104I CTCTTC 4 cut(s) 65, 290, 455, 571
EarI CTCTTC 4 cut(s) 65, 290, 455, 571
Ecl136II GAGCTC 1 cut(s) 85
EclXI CGGCCG 1 cut(s) 586
Eco130I CCWWGG 1 cut(s) 288
Eco147I AGGCCT 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 87
Eco52I CGGCCG 1 cut(s) 586
Eco53kI GAGCTC 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 12
EcoICRI GAGCTC 1 cut(s) 85
EcoRII CCWGG 3 cut(s) 59, 221, 574
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 259
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 288
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 3 cut(s) 25, 473, 637
FaiI YATR 4 cut(s) 23, 354, 471, 635
FatI CATG 3 cut(s) 21, 469, 633
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 1 cut(s) 87
FspBI CTAG 2 cut(s) 623, 676
GlaI GCGC 1 cut(s) 494
GsuI CTGGAG 3 cut(s) 63, 82, 629
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 4 cut(s) 59, 369, 422, 588
HhaI GCGC 1 cut(s) 495
Hin1II CATG 3 cut(s) 25, 473, 637
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HinfI GANTC 2 cut(s) 17, 502
HphI GGTGA 2 cut(s) 304, 428
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 6 cut(s) 12, 14, 239, 397, 570, 646
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 3 cut(s) 49, 84, 649
HpyCH4III ACNGT 2 cut(s) 114, 130
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 6 cut(s) 25, 257, 320, 440, 561, 593
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 428, 431, 437, 440, 443
HpyF3I CTNAG 2 cut(s) 408, 447
HpySE526I ACGT 1 cut(s) 34
Hsp92II CATG 3 cut(s) 25, 473, 637
HspAI GCGC 1 cut(s) 493
LguI GCTCTTC 1 cut(s) 455
LmnI GCTCC 2 cut(s) 648, 665
Lsp1109I GCAGC 9 cut(s) 106, 332, 373, 376, 418, 421, 424, 430, 452
LweI GCATC 3 cut(s) 266, 307, 570
MabI ACCWGGT 1 cut(s) 574
MaeI CTAG 2 cut(s) 623, 676
MaeII ACGT 1 cut(s) 34
MaeIII GTNAC 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 3 cut(s) 193, 363, 533
MlyI GAGTC 1 cut(s) 496
MmeI TCCRAC 1 cut(s) 465
Mph1103I ATGCAT 1 cut(s) 259
MseI TTAA 1 cut(s) 456
MslI CAYNNNNRTG 1 cut(s) 354
MspA1I CMGCKG 3 cut(s) 386, 431, 443
MspR9I CCNGG 3 cut(s) 61, 223, 576
MvaI CCWGG 3 cut(s) 61, 223, 576
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 6 cut(s) 91, 428, 431, 437, 440, 443
NlaIII CATG 3 cut(s) 25, 473, 637
NmuCI GTSAC 1 cut(s) 217
NsiI ATGCAT 1 cut(s) 259
NspI RCATGY 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 12
PceI AGGCCT 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 455
PfeI GAWTC 1 cut(s) 17
PflFI GACNNNGTC 1 cut(s) 576
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 3 cut(s) 59, 221, 574
PspGI CCWGG 3 cut(s) 59, 221, 574
PstI CTGCAG 1 cut(s) 442
PstNI CAGNNNCTG 1 cut(s) 446
PsyI GACNNNGTC 1 cut(s) 576
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 2 cut(s) 80, 280
RsaNI GTAC 2 cut(s) 79, 279
RseI CAYNNNNRTG 1 cut(s) 354
SacI GAGCTC 1 cut(s) 87
SapI GCTCTTC 1 cut(s) 455
SaqAI TTAA 1 cut(s) 456
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 3 cut(s) 61, 223, 576
SduI GDGCHC 1 cut(s) 87
SexAI ACCWGGT 1 cut(s) 574
SfaNI GCATC 3 cut(s) 266, 307, 570
SfcI CTRYAG 1 cut(s) 438
Sfr274I CTCGAG 1 cut(s) 12
SlaI CTCGAG 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 354
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
Sse9I AATT 3 cut(s) 193, 363, 533
SseBI AGGCCT 1 cut(s) 59
SsiI CCGC 6 cut(s) 370, 384, 423, 429, 543, 585
SspMI CTAG 2 cut(s) 623, 676
SstI GAGCTC 1 cut(s) 87
StuI AGGCCT 1 cut(s) 59
StyD4I CCNGG 3 cut(s) 59, 221, 574
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 2 cut(s) 114, 130
TaiI ACGT 1 cut(s) 37
TaqI TCGA 2 cut(s) 13, 148
TasI AATT 3 cut(s) 193, 363, 533
TauI GCSGC 3 cut(s) 372, 425, 588
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 3 cut(s) 46, 135, 341
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 9 cut(s) 94, 320, 386, 389, 431, 434, 437, 440, 443
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 650
TspRI CASTG 3 cut(s) 46, 135, 341
Tth111I GACNNNGTC 1 cut(s) 576
XapI RAATTY 1 cut(s) 193
XceI RCATGY 1 cut(s) 473
XhoI CTCGAG 1 cut(s) 12
XspI CTAG 2 cut(s) 623, 676
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.