Rorug05G0197800

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001913
Physical Location & Seq
Reverse (-)
19570460 .. 19572293
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0197800.1

Sequence Viewer

Length: 675 bp
ATGGCTGCCTCTATGTTTAAGCTCTCAGTTTTCTTGAGCCTGCTCTCCAATCTGAATTTGGGTCTCTGGCCAGAAGCTCAGAAAAGCGTGGCAACATTTCCACCATCATGTAACAGCATAGAGTGCCCGAGCTATGACTCCATCGAGGTTGGCAACGGCTATGAAGTTCGTCGTTACAATTCGAGCGAGTGGATATCAACTGCTCCTATTCAGGACATTTCTTTGGTTGCAGCCACCAGAACTGGCTTCTTACAGCTGTTTGATTACATTCAAGGAAAGAACAAGTATGGAGAGAAAATAGAGATGACTGCCCCAGTGCTTAGCGAAGTTCAACCAAGCGATGGACCATTCTGTGAGTCTTCATTTGTGGTGAGCTTCTATGTACCAAAAAAGAACCAAGCAAAACCACCACCAGCAGAAGGCCTCCATGTTCAGAAATGGACATCTACATATGTGGCTGTGAGACAATTCAGTGGGTTTGTTTCAGACTACGATGTTGGAGTAGAAGCTGCTGCCTTGCAGTCCAGTCTTGCAGGAACTGTTTGGTCTGCTGCCATTGAGAAAAGCCATGGAGCTGATCACACTACAATTTATACTGTTGCACAGTACAACTCTCCATTTGAATTTGATCACAGGGTGAATGAGATCTGGATTTCGTTTAATTTAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000313 GO:0000462 GO:0000956 GO:0002164 GO:0002181 GO:0002182 GO:0002376 GO:0002520 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0005840 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006414 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009547 GO:0009570 GO:0009790 GO:0009791 GO:0009792 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0012505 GO:0015031 GO:0015833 GO:0015935 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0019222 GO:0019439 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0030097 GO:0030490 GO:0030684 GO:0030686 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0034655 GO:0034660 GO:0035166 GO:0035167 GO:0042254 GO:0042255 GO:0042274 GO:0042886 GO:0043009 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043253 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048513 GO:0048519 GO:0048534 GO:0048542 GO:0048569 GO:0048731 GO:0048732 GO:0048856 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0090304 GO:1901360 GO:1901361 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N Ribosomal_S8e

Protein Analysis

224

Amino Acids

24.8

Weight (kDa)

4.93

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 40 - 218 5e-52 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 341
AcoI YGGCCR 1 cut(s) 68
AcsI RAATTY 2 cut(s) 55, 623
AdeI CACNNNGTG 1 cut(s) 637
AfaI GTAC 2 cut(s) 384, 608
AfiI CCNNNNNNNGG 2 cut(s) 341, 419
AgsI TTSAA 3 cut(s) 272, 332, 623
AluBI AGCT 7 cut(s) 22, 77, 132, 256, 375, 509, 575
AluI AGCT 7 cut(s) 22, 77, 132, 256, 375, 509, 575
Alw26I GTCTC 2 cut(s) 68, 457
AlwNI CAGNNNCTG 1 cut(s) 539
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 2 cut(s) 68, 421
ApeKI GCWGC 5 cut(s) 5, 230, 509, 512, 551
ApoI RAATTY 2 cut(s) 55, 623
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 382, 649
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 344
BaeGI GKGCMC 1 cut(s) 128
BalI TGGCCA 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 351
BbvI GCAGC 4 cut(s) 242, 496, 499, 538
BccI CCATC 3 cut(s) 112, 149, 335
BceAI ACGGC 1 cut(s) 172
BclI TGATCA 2 cut(s) 577, 628
BcoDI GTCTC 2 cut(s) 68, 457
BglII AGATCT 1 cut(s) 645
BisI GCNGC 5 cut(s) 6, 231, 510, 513, 552
BlpI GCTNAGC 1 cut(s) 320
BlsI GCNGC 5 cut(s) 7, 232, 511, 514, 553
Bme18I GGWCC 1 cut(s) 344
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 344
BmrI ACTGGG 1 cut(s) 308
BmuI ACTGGG 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 320
BpuEI CTTGAG 1 cut(s) 55
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 568
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 419
Bse1I ACTGG 3 cut(s) 247, 314, 525
BseDI CCNNGG 1 cut(s) 568
BseLI CCNNNNNNNGG 2 cut(s) 341, 419
BseMII CTCAG 2 cut(s) 39, 92
BseNI ACTGG 3 cut(s) 247, 314, 525
BseSI GKGCMC 1 cut(s) 128
BseXI GCAGC 4 cut(s) 242, 496, 499, 538
BshFI GGCC 2 cut(s) 70, 423
BsiHKCI CYCGRG 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 341, 419
BsmAI GTCTC 2 cut(s) 68, 457
BsnI GGCC 2 cut(s) 70, 423
Bso31I GGTCTC 1 cut(s) 68
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 3 cut(s) 577, 628, 645
Bsp1720I GCTNAGC 1 cut(s) 320
Bsp19I CCATGG 1 cut(s) 568
BspANI GGCC 2 cut(s) 70, 423
BspCNI CTCAG 2 cut(s) 38, 91
BspTNI GGTCTC 1 cut(s) 68
BsrI ACTGG 3 cut(s) 247, 314, 525
BssECI CCNNGG 1 cut(s) 568
BssMI GATC 3 cut(s) 577, 628, 645
BssT1I CCWWGG 1 cut(s) 568
Bst4CI ACNGT 3 cut(s) 541, 598, 606
Bst6I CTCTTC 1 cut(s) 663
BstAPI GCANNNNNTGC 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 3 cut(s) 25, 78, 320
BstDSI CCRYGG 1 cut(s) 568
BstKTI GATC 3 cut(s) 580, 631, 648
BstMAI GTCTC 2 cut(s) 68, 457
BstMBI GATC 3 cut(s) 577, 628, 645
BstMWI GCNNNNNNNGC 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 128
BstV1I GCAGC 4 cut(s) 242, 496, 499, 538
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 645
BstYI RGATCY 1 cut(s) 645
BsuRI GGCC 2 cut(s) 70, 423
BtgI CCRYGG 1 cut(s) 568
BtgZI GCGATG 1 cut(s) 354
BtsIMutI CAGTG 2 cut(s) 321, 478
Cac8I GCNNGC 1 cut(s) 41
CaiI CAGNNNCTG 1 cut(s) 539
Cfr13I GGNCC 1 cut(s) 344
Csp6I GTAC 2 cut(s) 383, 607
CviAII CATG 3 cut(s) 108, 428, 569
CviQI GTAC 2 cut(s) 383, 607
DdeI CTNAG 3 cut(s) 25, 78, 320
DpnI GATC 3 cut(s) 579, 630, 647
DpnII GATC 3 cut(s) 577, 628, 645
DraIII CACNNNGTG 1 cut(s) 637
EaeI YGGCCR 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
Eco130I CCWWGG 1 cut(s) 568
Eco147I AGGCCT 1 cut(s) 423
Eco31I GGTCTC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 127
EcoRV GATATC 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 568
FaeI CATG 3 cut(s) 111, 431, 572
FatI CATG 3 cut(s) 107, 427, 568
FauNDI CATATG 1 cut(s) 451
FbaI TGATCA 2 cut(s) 577, 628
Fnu4HI GCNGC 5 cut(s) 6, 231, 510, 513, 552
Fsp4HI GCNGC 5 cut(s) 6, 231, 510, 513, 552
GluI GCNGC 5 cut(s) 6, 231, 510, 513, 552
HaeIII GGCC 2 cut(s) 70, 423
Hin1II CATG 3 cut(s) 111, 431, 572
HinfI GANTC 2 cut(s) 137, 356
HphI GGTGA 2 cut(s) 382, 649
Hpy188I TCNGA 4 cut(s) 54, 81, 435, 487
Hpy188III TCNNGA 3 cut(s) 34, 212, 649
Hpy99I CGWCG 1 cut(s) 174
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 3 cut(s) 541, 598, 606
HpyCH4V TGCA 4 cut(s) 230, 520, 533, 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 123
HpyF3I CTNAG 3 cut(s) 25, 78, 320
Hsp92II CATG 3 cut(s) 111, 431, 572
Ksp22I TGATCA 2 cut(s) 577, 628
Kzo9I GATC 3 cut(s) 577, 628, 645
LmnI GCTCC 2 cut(s) 208, 572
Lsp1109I GCAGC 4 cut(s) 242, 496, 499, 538
MaeIII GTNAC 2 cut(s) 110, 173
MalI GATC 3 cut(s) 579, 630, 647
MboI GATC 3 cut(s) 577, 628, 645
MboII GAAGA 1 cut(s) 351
MflI RGATCY 1 cut(s) 645
MhlI GDGCHC 1 cut(s) 128
MlsI TGGCCA 1 cut(s) 70
MluCI AATT 6 cut(s) 55, 178, 467, 588, 623, 661
MluNI TGGCCA 1 cut(s) 70
MlyI GAGTC 2 cut(s) 131, 365
MmeI TCCRAC 1 cut(s) 478
MnlI CCTC 3 cut(s) 19, 139, 434
Mox20I TGGCCA 1 cut(s) 70
MscI TGGCCA 1 cut(s) 70
MseI TTAA 2 cut(s) 18, 660
MslI CAYNNNNRTG 1 cut(s) 106
Msp20I TGGCCA 1 cut(s) 70
MspA1I CMGCKG 1 cut(s) 256
MwoI GCNNNNNNNGC 1 cut(s) 123
NcoI CCATGG 1 cut(s) 568
NdeI CATATG 1 cut(s) 451
NdeII GATC 3 cut(s) 577, 628, 645
NlaIII CATG 3 cut(s) 111, 431, 572
PceI AGGCCT 1 cut(s) 423
PflMI CCANNNNNTGG 1 cut(s) 341
PkrI GCNGC 5 cut(s) 7, 232, 511, 514, 553
PleI GAGTC 2 cut(s) 131, 364
PpsI GAGTC 2 cut(s) 131, 364
PspPI GGNCC 1 cut(s) 344
PstNI CAGNNNCTG 1 cut(s) 539
PsuI RGATCY 1 cut(s) 645
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 2 cut(s) 384, 608
RsaNI GTAC 2 cut(s) 383, 607
RseI CAYNNNNRTG 1 cut(s) 106
SaqAI TTAA 2 cut(s) 18, 660
SatI GCNGC 5 cut(s) 6, 231, 510, 513, 552
Sau3AI GATC 3 cut(s) 577, 628, 645
Sau96I GGNCC 1 cut(s) 344
SchI GAGTC 2 cut(s) 131, 365
SduI GDGCHC 1 cut(s) 128
SetI ASST 8 cut(s) 24, 79, 134, 150, 258, 377, 511, 577
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 6 cut(s) 55, 178, 467, 588, 623, 661
SseBI AGGCCT 1 cut(s) 423
StuI AGGCCT 1 cut(s) 423
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 3 cut(s) 541, 598, 606
TaqI TCGA 2 cut(s) 144, 182
TasI AATT 6 cut(s) 55, 178, 467, 588, 623, 661
TatI WGTACW 1 cut(s) 606
Tru1I TTAA 2 cut(s) 18, 660
Tru9I TTAA 2 cut(s) 18, 660
TscAI CASTG 2 cut(s) 321, 478
TseI GCWGC 5 cut(s) 5, 230, 509, 512, 551
TspDTI ATGAA 2 cut(s) 177, 351
TspRI CASTG 2 cut(s) 321, 478
Van91I CCANNNNNTGG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 344
XapI RAATTY 2 cut(s) 55, 623
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.