Rw0G001400

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00043
OG0001913
Physical Location & Seq
Reverse (-)
85767 .. 87894
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001400.1

Sequence Viewer

Length: 678 bp
ATGGGTATCTCTCGAGATTCCATGCACAAGAGACGTGCCACTGGAGGCAAGAAGAAGGCGTGGAGGAAGAAGCGAAAGTATGAGCTCGGAAGGGTGCCTGCCAACACTAAACTGTCCAGCAACAAGACAGTGAGGAGGATCAGGGTTCGAGGTGGAAATGTGAAGTGGCGTGCTTTGAGGCTCGACACAGGAAATTTCTCTTGGGGTAGTGAGACTGTGACCAGGAAGACCCGTCTTCTTGATGTTGTCTACAATGCATCTAACAATGAGCTGGTGCGTACTCAAACCTTGGTGAAGGGTGCCATTGTTCAGGTTGATGCAGCACCTTTCAAGCAGTGGTATTCTACCCACTATGGTGTTGAAATTGGGCGCAGGAAGAAAACTGCAGCTGTTATCAAGAAAGAAGCAGAGGACGGTGAGGCCGCTACTGCCGCTGCCGCTGCCGCTGAGGAGGTGAAGAAGAGCAAGCATGTAGCAAGGAAGCTAGAGAAGCGTCAGCAGACTCGTGTGCTTGACCAGCATATTGAGGAACAATTTGGTGGTGGTCGTCTATTGGCTTGCATCTCTTCACGACCTGGTCAATGTGGCCGTGCAGATGGATACATCTTGGAGGGCAAAGAGCTGGAGTTTTACATGAAGAAGCTCCAGAAGAAGAAGGGAAAGGGAGCAGCTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000313 GO:0000462 GO:0000956 GO:0002164 GO:0002181 GO:0002182 GO:0002376 GO:0002520 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0005840 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006414 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009547 GO:0009570 GO:0009790 GO:0009791 GO:0009792 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0012505 GO:0015031 GO:0015833 GO:0015935 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0019222 GO:0019439 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0030097 GO:0030490 GO:0030684 GO:0030686 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0034655 GO:0034660 GO:0035166 GO:0035167 GO:0042254 GO:0042255 GO:0042274 GO:0042886 GO:0043009 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043253 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048513 GO:0048519 GO:0048534 GO:0048542 GO:0048569 GO:0048731 GO:0048732 GO:0048856 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0090304 GO:1901360 GO:1901361 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N Ribosomal_S8e

Protein Analysis

225

Amino Acids

25.21

Weight (kDa)

10.49

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8e PF01201 5 - 203 2.6e-52 Ribosomal protein S8e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 94, 299
AccI GTMKAC 1 cut(s) 249
AciI CCGC 4 cut(s) 423, 432, 438, 444
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 1 cut(s) 193
AfaI GTAC 1 cut(s) 280
AgsI TTSAA 2 cut(s) 331, 362
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 2 cut(s) 221, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 184, 216
AleI CACNNNNGTG 1 cut(s) 354
AluBI AGCT 7 cut(s) 85, 271, 389, 484, 622, 643, 671
AluI AGCT 7 cut(s) 85, 271, 389, 484, 622, 643, 671
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 2 cut(s) 25, 206
AlwI GGATC 1 cut(s) 146
Ama87I CYCGRG 1 cut(s) 12
AoxI GGCC 2 cut(s) 420, 586
ApeKI GCWGC 6 cut(s) 320, 386, 434, 440, 668, 671
ApoI RAATTY 1 cut(s) 193
AspLEI GCGC 1 cut(s) 372
AsuHPI GGTGA 3 cut(s) 304, 428, 466
AvaI CYCGRG 1 cut(s) 12
BanI GGYRCC 2 cut(s) 94, 299
BanII GRGCYC 1 cut(s) 87
BauI CACGAG 1 cut(s) 504
BbsI GAAGAC 2 cut(s) 227, 233
Bbv12I GWGCWC 1 cut(s) 87
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 5 cut(s) 332, 398, 421, 427, 658
BccI CCATC 1 cut(s) 590
BceAI ACGGC 1 cut(s) 573
BciT130I CCWGG 2 cut(s) 223, 576
BciVI GTATCC 1 cut(s) 593
BcoDI GTCTC 2 cut(s) 25, 206
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 1 cut(s) 384
BfuI GTATCC 1 cut(s) 593
Bme1390I CCNGG 2 cut(s) 223, 576
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 35
BmiI GGNNCC 2 cut(s) 96, 301
BmrFI CCNGG 2 cut(s) 223, 576
BmsI GCATC 3 cut(s) 266, 307, 570
BpiI GAAGAC 2 cut(s) 227, 233
BpmI CTGGAG 3 cut(s) 63, 629, 644
Bpu10I CCTNAGC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 288
Bse1I ACTGG 1 cut(s) 46
BseBI CCWGG 2 cut(s) 223, 576
BseDI CCNNGG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 1 cut(s) 46
BseRI GAGGAG 2 cut(s) 148, 464
BseXI GCAGC 5 cut(s) 332, 398, 421, 427, 658
BsgI GTGCAG 1 cut(s) 612
BshFI GGCC 2 cut(s) 422, 588
BshNI GGYRCC 2 cut(s) 94, 299
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 12
BsmAI GTCTC 2 cut(s) 25, 206
BsmBI CGTCTC 1 cut(s) 25
BsnI GGCC 2 cut(s) 422, 588
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 1 cut(s) 138
BspACI CCGC 4 cut(s) 423, 432, 438, 444
BspANI GGCC 2 cut(s) 422, 588
BspCNI CTCAG 1 cut(s) 439
BspLI GGNNCC 2 cut(s) 96, 301
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 1 cut(s) 146
BspQI GCTCTTC 1 cut(s) 455
BspT107I GGYRCC 2 cut(s) 94, 299
BsrI ACTGG 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 1 cut(s) 138
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 288
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 2 cut(s) 223, 576
Bst4CI ACNGT 4 cut(s) 114, 130, 217, 416
Bst6I CTCTTC 2 cut(s) 455, 571
BstC8I GCNNGC 4 cut(s) 99, 171, 467, 559
BstDEI CTNAG 1 cut(s) 447
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 1 cut(s) 141
BstMAI GTCTC 2 cut(s) 25, 206
BstMBI GATC 1 cut(s) 138
BstMWI GCNNNNNNNGC 7 cut(s) 428, 431, 437, 440, 443, 490, 517
BstNI CCWGG 2 cut(s) 223, 576
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 221, 574
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 5 cut(s) 332, 398, 421, 427, 658
BstV2I GAAGAC 2 cut(s) 227, 233
BsuI GTATCC 1 cut(s) 593
BsuRI GGCC 2 cut(s) 422, 588
BtrI CACGTC 1 cut(s) 35
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 3 cut(s) 39, 135, 341
Cac8I GCNNGC 4 cut(s) 99, 171, 467, 559
CfoI GCGC 1 cut(s) 372
CseI GACGC 1 cut(s) 482
CsiI ACCWGGT 1 cut(s) 574
Csp6I GTAC 1 cut(s) 279
CviAII CATG 3 cut(s) 22, 470, 634
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 1 cut(s) 447
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 586
Eam1104I CTCTTC 2 cut(s) 455, 571
EarI CTCTTC 2 cut(s) 455, 571
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 12
EcoICRI GAGCTC 1 cut(s) 85
EcoRII CCWGG 2 cut(s) 221, 574
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 259
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 288
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 3 cut(s) 25, 473, 637
FaiI YATR 6 cut(s) 23, 81, 354, 471, 522, 635
FatI CATG 3 cut(s) 21, 469, 633
FblI GTMKAC 1 cut(s) 249
FriOI GRGCYC 1 cut(s) 87
FspBI CTAG 1 cut(s) 485
GlaI GCGC 1 cut(s) 371
GsuI CTGGAG 3 cut(s) 63, 629, 644
HaeIII GGCC 2 cut(s) 422, 588
HgaI GACGC 1 cut(s) 482
HhaI GCGC 1 cut(s) 372
Hin1II CATG 3 cut(s) 25, 473, 637
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HinfI GANTC 2 cut(s) 17, 502
HphI GGTGA 3 cut(s) 304, 428, 466
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 6 cut(s) 12, 14, 239, 397, 570, 646
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 4 cut(s) 49, 84, 289, 649
HpyCH4III ACNGT 4 cut(s) 114, 130, 217, 416
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 6 cut(s) 25, 257, 320, 386, 561, 593
HpyF10VI GCNNNNNNNGC 7 cut(s) 428, 431, 437, 440, 443, 490, 517
HpyF3I CTNAG 1 cut(s) 447
HpySE526I ACGT 1 cut(s) 34
Hsp92II CATG 3 cut(s) 25, 473, 637
HspAI GCGC 1 cut(s) 370
Kzo9I GATC 1 cut(s) 138
LguI GCTCTTC 1 cut(s) 455
LmnI GCTCC 2 cut(s) 648, 665
Lsp1109I GCAGC 5 cut(s) 332, 398, 421, 427, 658
LweI GCATC 3 cut(s) 266, 307, 570
MabI ACCWGGT 1 cut(s) 574
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 34
MaeIII GTNAC 1 cut(s) 217
MalI GATC 1 cut(s) 140
MboI GATC 1 cut(s) 138
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 3 cut(s) 193, 363, 533
MlyI GAGTC 1 cut(s) 496
Mph1103I ATGCAT 1 cut(s) 259
MslI CAYNNNNRTG 1 cut(s) 354
MspA1I CMGCKG 5 cut(s) 389, 434, 440, 446, 671
MspR9I CCNGG 2 cut(s) 223, 576
MvaI CCWGG 2 cut(s) 223, 576
MwoI GCNNNNNNNGC 7 cut(s) 428, 431, 437, 440, 443, 490, 517
NdeII GATC 1 cut(s) 138
NlaIII CATG 3 cut(s) 25, 473, 637
NlaIV GGNNCC 2 cut(s) 96, 301
NmuCI GTSAC 1 cut(s) 217
NsiI ATGCAT 1 cut(s) 259
NspI RCATGY 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 455
PfeI GAWTC 1 cut(s) 17
PflFI GACNNNGTC 1 cut(s) 576
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 221, 574
PspGI CCWGG 2 cut(s) 221, 574
PspN4I GGNNCC 2 cut(s) 96, 301
PstI CTGCAG 1 cut(s) 388
PsyI GACNNNGTC 1 cut(s) 576
PvuII CAGCTG 2 cut(s) 389, 671
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
RseI CAYNNNNRTG 1 cut(s) 354
SacI GAGCTC 1 cut(s) 87
SapI GCTCTTC 1 cut(s) 455
Sau3AI GATC 1 cut(s) 138
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 2 cut(s) 223, 576
SduI GDGCHC 1 cut(s) 87
SexAI ACCWGGT 1 cut(s) 574
SfaNI GCATC 3 cut(s) 266, 307, 570
SfcI CTRYAG 1 cut(s) 384
Sfr274I CTCGAG 1 cut(s) 12
SlaI CTCGAG 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 354
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
Sse9I AATT 3 cut(s) 193, 363, 533
SsiI CCGC 4 cut(s) 423, 432, 438, 444
SspMI CTAG 1 cut(s) 485
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 221, 574
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 4 cut(s) 114, 130, 217, 416
TaiI ACGT 1 cut(s) 37
TaqI TCGA 3 cut(s) 13, 148, 183
TasI AATT 3 cut(s) 193, 363, 533
TauI GCSGC 4 cut(s) 425, 434, 440, 446
TfiI GAWTC 1 cut(s) 17
TscAI CASTG 3 cut(s) 46, 135, 341
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 6 cut(s) 320, 386, 434, 440, 668, 671
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 650
TspRI CASTG 3 cut(s) 46, 135, 341
Tth111I GACNNNGTC 1 cut(s) 576
XapI RAATTY 1 cut(s) 193
XceI RCATGY 1 cut(s) 473
XhoI CTCGAG 1 cut(s) 12
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 1 cut(s) 485
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.