FvH4_3g28221

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004520
Physical Location & Seq
Forward (+)
21184442 .. 21185776
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28221.t1

Sequence Viewer

Length: 819 bp
ATGACTCATGGAGGAGATAAACTGGATAGACGGCCTCATGACCTAGGTGAATATCACAATCTTTATCTGTGCTATTTGTCTTGGAAGAGAGTTAGCAAACTAGCTAGCAGTTATATATACATTGAAAGTTTGAATTCTGTGTTAGCCCCAATCTTCTTCAATGTGGAACATGTATTGACTGAAGAAGAGAATTGTGTAATTGAAGAAGAGGAAGACATGTATGAAGGGGAAGATGATCAATTGCAACAGAACCCTGGATATGAACATGGAAGTGAGAGTGACGACTCCGACTATGAATTGTATGATGAAGAAAATGATGTTGCTATAGAGGACGATCATTTATTTGATGACACTATTGTGTTAACTCCATTACGGAGACCAAATGTTGAAGATGATGATACTTTTTCACTAAACTGTTCAGAAGAGAATATCTTGTTGGAAGAGGAATATCCCATGGAATCAGGTTCCCTATTACCATTGAATTTCATGCAAGATAACATTGCACAAGAGAGAGAATATGATTCTGATGATTTGAACAGTCCAGTGAATTCAGATGATGAAGAACAAGGAATGACTAAATATCCTGAGTTCAACGCTGCCACAGATATGGAGAACCCTGTATTTGTGATTGGAATGTTGTTTGCTACAAAGAATGATGTGAAGGAAGCAATAAAGGAGTATTCAATCAAAGGAGGTTATGAGACAACTATAAAAAAGAATGATAGGATCCGAATTATGGCAGAGTGTATCCCAGTTTGTCCTTGGAAAATACGTGCAGCTCCTAATGATGAAGGTACATGGCAAATCAAATCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

273

Amino Acids

31.42

Weight (kDa)

4.14

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBD_Tnp_Mut PF03108 209 - 261 5.9e-09 MuDR family transposase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 721, 734
AcsI RAATTY 3 cut(s) 133, 481, 547
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 1 cut(s) 796
AfiI CCNNNNNNNGG 1 cut(s) 736
AflIII ACRYGT 2 cut(s) 169, 216
AgsI TTSAA 9 cut(s) 125, 133, 160, 203, 389, 481, 535, 592, 684
AjnI CCWGG 1 cut(s) 253
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AleI CACNNNNGTG 1 cut(s) 356
AluBI AGCT 2 cut(s) 104, 779
AluI AGCT 2 cut(s) 104, 779
Alw26I GTCTC 2 cut(s) 370, 695
AlwI GGATC 2 cut(s) 721, 734
AoxI GGCC 1 cut(s) 32
ApeKI GCWGC 2 cut(s) 596, 776
ApoI RAATTY 3 cut(s) 133, 481, 547
AspA2I CCTAGG 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 59
AsuNHI GCTAGC 1 cut(s) 104
AvrII CCTAGG 1 cut(s) 43
BamHI GGATCC 1 cut(s) 726
BbsI GAAGAC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 583, 788
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 255
BciVI GTATCC 1 cut(s) 758
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 2 cut(s) 370, 695
BfaI CTAG 3 cut(s) 44, 101, 105
BfmI CTRYAG 1 cut(s) 324
BfuI GTATCC 1 cut(s) 758
BisI GCNGC 2 cut(s) 597, 777
BlnI CCTAGG 1 cut(s) 43
BlsI GCNGC 2 cut(s) 598, 778
Bme1390I CCNGG 1 cut(s) 255
BmiI GGNNCC 2 cut(s) 466, 728
BmrFI CCNGG 1 cut(s) 255
BmrI ACTGGG 1 cut(s) 746
BmtI GCTAGC 1 cut(s) 108
BmuI ACTGGG 1 cut(s) 746
BpiI GAAGAC 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 773
BsaI GGTCTC 1 cut(s) 370
BsaJI CCNNGG 4 cut(s) 43, 253, 453, 761
Bsc4I CCNNNNNNNGG 1 cut(s) 736
Bse1I ACTGG 3 cut(s) 27, 542, 752
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 4 cut(s) 43, 253, 453, 761
BseLI CCNNNNNNNGG 1 cut(s) 736
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 576
BseNI ACTGG 3 cut(s) 27, 542, 752
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 583, 788
BsgI GTGCAG 1 cut(s) 795
BshFI GGCC 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 736
BsmAI GTCTC 2 cut(s) 370, 695
BsnI GGCC 1 cut(s) 34
Bso31I GGTCTC 1 cut(s) 370
Bsp143I GATC 3 cut(s) 235, 334, 726
Bsp19I CCATGG 1 cut(s) 453
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 1 cut(s) 577
BspHI TCATGA 1 cut(s) 37
BspLI GGNNCC 2 cut(s) 466, 728
BspOI GCTAGC 1 cut(s) 108
BspPI GGATC 2 cut(s) 721, 734
BspTNI GGTCTC 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 498
BsrI ACTGG 3 cut(s) 27, 542, 752
BssECI CCNNGG 4 cut(s) 43, 253, 453, 761
BssMI GATC 3 cut(s) 235, 334, 726
BssT1I CCWWGG 3 cut(s) 43, 453, 761
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 2 cut(s) 416, 539
Bst6I CTCTTC 5 cut(s) 80, 180, 201, 417, 435
BstBAI YACGTR 1 cut(s) 773
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 585
BstDSI CCRYGG 1 cut(s) 453
BstKTI GATC 3 cut(s) 238, 337, 729
BstMAI GTCTC 2 cut(s) 370, 695
BstMBI GATC 3 cut(s) 235, 334, 726
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 2 cut(s) 173, 220
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 2 cut(s) 583, 788
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 726
BstXI CCANNNNNNTGG 1 cut(s) 607
BstYI RGATCY 1 cut(s) 726
BsuI GTATCC 1 cut(s) 758
BsuRI GGCC 1 cut(s) 34
BtgI CCRYGG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 549
Cac8I GCNNGC 1 cut(s) 106
CciI TCATGA 1 cut(s) 37
Csp6I GTAC 1 cut(s) 795
CviAII CATG 8 cut(s) 8, 38, 170, 217, 266, 454, 487, 798
CviJI RGCY 4 cut(s) 34, 104, 146, 779
CviKI_1 RGCY 4 cut(s) 34, 104, 146, 779
CviQI GTAC 1 cut(s) 795
DdeI CTNAG 1 cut(s) 585
DpnI GATC 3 cut(s) 237, 336, 728
DpnII GATC 3 cut(s) 235, 334, 726
Eam1104I CTCTTC 5 cut(s) 80, 180, 201, 417, 435
EarI CTCTTC 5 cut(s) 80, 180, 201, 417, 435
Eco130I CCWWGG 3 cut(s) 43, 453, 761
Eco31I GGTCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 201
EcoRI GAATTC 2 cut(s) 133, 547
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 3 cut(s) 43, 453, 761
ErhI CCWWGG 3 cut(s) 43, 453, 761
FaeI CATG 8 cut(s) 11, 41, 173, 220, 269, 457, 490, 801
FatI CATG 8 cut(s) 7, 37, 169, 216, 265, 453, 486, 797
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 2 cut(s) 597, 777
Fsp4HI GCNGC 2 cut(s) 597, 777
FspBI CTAG 3 cut(s) 44, 101, 105
GluI GCNGC 2 cut(s) 597, 777
HaeIII GGCC 1 cut(s) 34
Hin1II CATG 8 cut(s) 11, 41, 173, 220, 269, 457, 490, 801
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HinfI GANTC 4 cut(s) 4, 284, 458, 521
HpaI GTTAAC 1 cut(s) 363
HphI GGTGA 1 cut(s) 59
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 5 cut(s) 289, 421, 526, 553, 731
Hpy188III TCNNGA 2 cut(s) 38, 584
Hpy8I GTNNAC 1 cut(s) 363
HpyAV CCTTC 3 cut(s) 218, 655, 785
HpyCH4III ACNGT 2 cut(s) 416, 539
HpyCH4IV ACGT 1 cut(s) 772
HpyCH4V TGCA 4 cut(s) 244, 490, 503, 776
HpyF3I CTNAG 1 cut(s) 585
HpySE526I ACGT 1 cut(s) 772
Hsp92II CATG 8 cut(s) 11, 41, 173, 220, 269, 457, 490, 801
Ksp22I TGATCA 1 cut(s) 235
KspAI GTTAAC 1 cut(s) 363
Kzo9I GATC 3 cut(s) 235, 334, 726
LmnI GCTCC 1 cut(s) 784
LpnPI CCDG 8 cut(s) 8, 240, 267, 447, 555, 597, 630, 765
Lsp1109I GCAGC 2 cut(s) 583, 788
MaeI CTAG 3 cut(s) 44, 101, 105
MaeII ACGT 1 cut(s) 772
MaeIII GTNAC 1 cut(s) 278
MalI GATC 3 cut(s) 237, 336, 728
MboI GATC 3 cut(s) 235, 334, 726
MfeI CAATTG 1 cut(s) 239
MflI RGATCY 1 cut(s) 726
MluCI AATT 8 cut(s) 133, 190, 198, 239, 296, 481, 547, 732
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 2 cut(s) 312, 417
MnlI CCTC 6 cut(s) 5, 45, 202, 322, 436, 686
MseI TTAA 1 cut(s) 362
MslI CAYNNNNRTG 3 cut(s) 270, 356, 605
MspR9I CCNGG 1 cut(s) 255
MunI CAATTG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 255
NcoI CCATGG 1 cut(s) 453
NdeII GATC 3 cut(s) 235, 334, 726
NheI GCTAGC 1 cut(s) 104
NlaIII CATG 8 cut(s) 11, 41, 173, 220, 269, 457, 490, 801
NlaIV GGNNCC 2 cut(s) 466, 728
NmuCI GTSAC 1 cut(s) 278
NspI RCATGY 2 cut(s) 173, 220
OliI CACNNNNGTG 1 cut(s) 356
PagI TCATGA 1 cut(s) 37
PciI ACATGT 2 cut(s) 169, 216
PfeI GAWTC 2 cut(s) 458, 521
PkrI GCNGC 2 cut(s) 598, 778
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
Ppu21I YACGTR 1 cut(s) 773
PscI ACATGT 2 cut(s) 169, 216
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 466, 728
PsuI RGATCY 1 cut(s) 726
RsaI GTAC 1 cut(s) 796
RsaNI GTAC 1 cut(s) 795
RseI CAYNNNNRTG 3 cut(s) 270, 356, 605
SaqAI TTAA 1 cut(s) 362
SatI GCNGC 2 cut(s) 597, 777
Sau3AI GATC 3 cut(s) 235, 334, 726
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 1 cut(s) 255
SetI ASST 8 cut(s) 45, 49, 106, 466, 697, 775, 781, 796
SfcI CTRYAG 1 cut(s) 324
SmiMI CAYNNNNRTG 3 cut(s) 270, 356, 605
Sse9I AATT 8 cut(s) 133, 190, 198, 239, 296, 481, 547, 732
SspMI CTAG 3 cut(s) 44, 101, 105
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 3 cut(s) 43, 453, 761
TaaI ACNGT 2 cut(s) 416, 539
TaiI ACGT 1 cut(s) 775
TasI AATT 8 cut(s) 133, 190, 198, 239, 296, 481, 547, 732
TfiI GAWTC 2 cut(s) 458, 521
Tru1I TTAA 1 cut(s) 362
Tru9I TTAA 1 cut(s) 362
TscAI CASTG 1 cut(s) 549
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 2 cut(s) 596, 776
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 7 cut(s) 237, 276, 309, 321, 475, 573, 804
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 1 cut(s) 549
XapI RAATTY 3 cut(s) 133, 481, 547
XceI RCATGY 2 cut(s) 173, 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 759
XmaJI CCTAGG 1 cut(s) 43
XspI CTAG 3 cut(s) 44, 101, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.