FvH4_3g31250

Region found in RelA / SpoT proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003029
Physical Location & Seq
Forward (+)
25118354 .. 25120329
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31250.t1

Sequence Viewer

Length: 762 bp
ATGAGTTCGACGAGTTCCAAGCGGCCGAGGCATCTGATCCGTCTTCCGGCATCCAATCCCCATCTTCTCAAAACTCATATTCTCAGACGAAGAACCCTTCACCGTAGTCCAGCTAGCTTCAAAGCTGATATTCTCCCAAAGCCTATAGGTTTCACAAAGGGACCTCCACGGATGGGGATTTCAATTTCTGGTCGAAGATGGGGAAATTGGAAGGTGCAGTGCAACCGGAGCTCGAAATTGAAGTACCGGAAAACCGTGGCCATTGATGGAGAATATCAAAATGAAGCGAATTGCTCTCTGTGTGATTCTAATTGTTCATCATTGTTTCTGATAAGGAATCCCAGGGATCCAAAGGAATTGGATTGGGAGTTCGTTGCTTCTGGTTTACTACATGACACAGTCGAAGATACCAATGTTGTTACCTTTGAAAGGATAGAGGAGGAGTTTGGTGCTACTGTTCGTCATATTGTAGAAGGAGAGACTAAGGTGTCAAAGTTGGGAAAGTTGAAACGTAAGGGTGAACACGATTTTGTTCAAGATGTTAAAGCATATGATCTACGGCAGATGCTTCTAGCCATGACAGAGGAGTCCAGCATTACAAGGGAAACATTGCAGGTCTTTGCTCCTCTAGCAAAGTTGCTTGGGATCGATGTACCAAATCAAGATTGTGGAAGGTCTTATACAGAAAGCCAAAGAGGGTTGGATGTGATTGATACTTCTGGAATGGTCATGAACCTTCTCCAGGAAATGTACTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
HD_4 RelA_SpoT TGS

Protein Analysis

254

Amino Acids

28.84

Weight (kDa)

9.17

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 120 - 195 1e-11 HD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 487, 586
Acc36I ACCTGC 1 cut(s) 604
AciI CCGC 1 cut(s) 22
AclWI GGATC 4 cut(s) 31, 341, 354, 653
AcoI YGGCCR 2 cut(s) 23, 258
AfaI GTAC 3 cut(s) 245, 654, 752
AfiI CCNNNNNNNGG 4 cut(s) 46, 173, 429, 742
AgsI TTSAA 6 cut(s) 121, 183, 241, 428, 508, 536
AjnI CCWGG 2 cut(s) 341, 741
AloI GAACNNNNNNTCC 2 cut(s) 353, 385
AluBI AGCT 4 cut(s) 113, 117, 125, 231
AluI AGCT 4 cut(s) 113, 117, 125, 231
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 473
AlwI GGATC 4 cut(s) 31, 341, 354, 653
AoxI GGCC 2 cut(s) 23, 258
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 92, 530
AsuNHI GCTAGC 1 cut(s) 113
AvaII GGWCC 1 cut(s) 161
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BalI TGGCCA 1 cut(s) 260
BamHI GGATCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 233
BarI GAAGNNNNNNTAC 2 cut(s) 664, 696
BbsI GAAGAC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 233
BccI CCATC 4 cut(s) 69, 166, 192, 260
BceAI ACGGC 1 cut(s) 575
BciT130I CCWGG 2 cut(s) 343, 743
BcoDI GTCTC 1 cut(s) 473
BfaI CTAG 3 cut(s) 114, 572, 629
BfmI CTRYAG 1 cut(s) 144
BfuAI ACCTGC 1 cut(s) 604
BisI GCNGC 1 cut(s) 23
BlsI GCNGC 1 cut(s) 24
Bme1390I CCNGG 2 cut(s) 343, 743
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 162, 348
BmrFI CCNGG 2 cut(s) 343, 743
BmsI GCATC 3 cut(s) 40, 59, 555
BmtI GCTAGC 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 725
Bsa29I ATCGAT 1 cut(s) 648
BsaJI CCNNGG 5 cut(s) 26, 167, 255, 341, 342
BsaWI WCCGGW 2 cut(s) 225, 246
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 173, 429, 742
Bse3DI GCAATG 1 cut(s) 608
BseBI CCWGG 2 cut(s) 343, 743
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 5 cut(s) 26, 167, 255, 341, 342
BseGI GGATG 3 cut(s) 50, 177, 709
BseLI CCNNNNNNNGG 4 cut(s) 46, 173, 429, 742
BseMI GCAATG 1 cut(s) 608
BseMII CTCAG 1 cut(s) 97
BseRI GAGGAG 4 cut(s) 452, 455, 599, 615
BseX3I CGGCCG 1 cut(s) 23
BsgI GTGCAG 1 cut(s) 236
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 2 cut(s) 25, 260
BshVI ATCGAT 1 cut(s) 648
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 233
BsiSI CCGG 3 cut(s) 47, 226, 247
BslFI GGGAC 1 cut(s) 174
BslI CCNNNNNNNGG 4 cut(s) 46, 173, 429, 742
BsmAI GTCTC 1 cut(s) 473
BsmFI GGGAC 1 cut(s) 174
BsnI GGCC 2 cut(s) 25, 260
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 4 cut(s) 36, 346, 553, 645
BspACI CCGC 1 cut(s) 22
BspANI GGCC 2 cut(s) 25, 260
BspCNI CTCAG 1 cut(s) 96
BspDI ATCGAT 1 cut(s) 648
BspHI TCATGA 1 cut(s) 729
BspLI GGNNCC 2 cut(s) 162, 348
BspMI ACCTGC 1 cut(s) 604
BspOI GCTAGC 1 cut(s) 117
BspPI GGATC 4 cut(s) 31, 341, 354, 653
BsrDI GCAATG 1 cut(s) 608
BssECI CCNNGG 5 cut(s) 26, 167, 255, 341, 342
BssMI GATC 4 cut(s) 36, 346, 553, 645
Bst2UI CCWGG 2 cut(s) 343, 743
Bst4CI ACNGT 4 cut(s) 104, 256, 400, 457
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 2 cut(s) 83, 483
BstDSI CCRYGG 2 cut(s) 167, 255
BstENI CCTNNNNNAGG 2 cut(s) 427, 740
BstF5I GGATG 3 cut(s) 50, 177, 709
BstKTI GATC 4 cut(s) 39, 349, 556, 648
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 4 cut(s) 36, 346, 553, 645
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 3 cut(s) 28, 228, 629
BstNI CCWGG 2 cut(s) 343, 743
BstSCI CCNGG 2 cut(s) 341, 741
BstSFI CTRYAG 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 35
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BstZI CGGCCG 1 cut(s) 23
Bsu15I ATCGAT 1 cut(s) 648
BsuRI GGCC 2 cut(s) 25, 260
BsuTUI ATCGAT 1 cut(s) 648
BtgI CCRYGG 2 cut(s) 167, 255
BtsCI GGATG 3 cut(s) 50, 177, 709
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 1 cut(s) 224
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 115
CciI TCATGA 1 cut(s) 729
Cfr13I GGNCC 1 cut(s) 161
ClaI ATCGAT 1 cut(s) 648
Csp6I GTAC 3 cut(s) 244, 653, 751
CviAII CATG 3 cut(s) 392, 577, 730
CviJI RGCY 9 cut(s) 25, 113, 117, 125, 142, 231, 260, 575, 690
CviKI_1 RGCY 9 cut(s) 25, 113, 117, 125, 142, 231, 260, 575, 690
CviQI GTAC 3 cut(s) 244, 653, 751
DdeI CTNAG 2 cut(s) 83, 483
DpnI GATC 4 cut(s) 38, 348, 555, 647
DpnII GATC 4 cut(s) 36, 346, 553, 645
DrdI GACNNNNNNGTC 2 cut(s) 487, 586
DseDI GACNNNNNNGTC 2 cut(s) 487, 586
EaeI YGGCCR 2 cut(s) 23, 258
EagI CGGCCG 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 231
EclXI CGGCCG 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 161
Eco52I CGGCCG 1 cut(s) 23
Eco53kI GAGCTC 1 cut(s) 231
EcoICRI GAGCTC 1 cut(s) 231
EcoNI CCTNNNNNAGG 2 cut(s) 427, 740
EcoO109I RGGNCCY 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 341, 741
EcoT38I GRGCYC 1 cut(s) 233
FaeI CATG 3 cut(s) 395, 580, 733
FaiI YATR 9 cut(s) 78, 146, 393, 465, 550, 552, 578, 681, 731
FaqI GGGAC 1 cut(s) 174
FatI CATG 3 cut(s) 391, 576, 729
FauNDI CATATG 1 cut(s) 550
Fnu4HI GCNGC 1 cut(s) 23
FokI GGATG 3 cut(s) 37, 184, 716
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 1 cut(s) 23
FspBI CTAG 3 cut(s) 114, 572, 629
GluI GCNGC 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 725
HaeIII GGCC 2 cut(s) 25, 260
HapII CCGG 3 cut(s) 47, 226, 247
Hin1II CATG 3 cut(s) 395, 580, 733
HinfI GANTC 3 cut(s) 305, 337, 587
HpaII CCGG 3 cut(s) 47, 226, 247
HphI GGTGA 2 cut(s) 92, 530
Hpy166II GTNNAC 2 cut(s) 386, 521
Hpy188I TCNGA 3 cut(s) 36, 86, 330
Hpy188III TCNNGA 4 cut(s) 536, 662, 720, 730
Hpy8I GTNNAC 2 cut(s) 386, 521
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 5 cut(s) 107, 205, 467, 666, 746
HpyCH4III ACNGT 4 cut(s) 104, 256, 400, 457
HpyCH4IV ACGT 1 cut(s) 511
HpyCH4V TGCA 3 cut(s) 217, 222, 613
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 228, 629
HpyF3I CTNAG 2 cut(s) 83, 483
HpySE526I ACGT 1 cut(s) 511
Hsp92II CATG 3 cut(s) 395, 580, 733
Kzo9I GATC 4 cut(s) 36, 346, 553, 645
LmnI GCTCC 2 cut(s) 228, 628
LweI GCATC 3 cut(s) 40, 59, 555
MaeI CTAG 3 cut(s) 114, 572, 629
MaeII ACGT 1 cut(s) 511
MaeIII GTNAC 1 cut(s) 418
MalI GATC 4 cut(s) 38, 348, 555, 647
MboI GATC 4 cut(s) 36, 346, 553, 645
MboII GAAGA 5 cut(s) 35, 56, 102, 207, 416
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 6 cut(s) 183, 205, 236, 289, 310, 356
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 1 cut(s) 681
MnlI CCTC 7 cut(s) 21, 174, 430, 433, 577, 636, 689
Mox20I TGGCCA 1 cut(s) 260
MscI TGGCCA 1 cut(s) 260
MseI TTAA 1 cut(s) 543
Msp20I TGGCCA 1 cut(s) 260
MspI CCGG 3 cut(s) 47, 226, 247
MspR9I CCNGG 2 cut(s) 343, 743
MvaI CCWGG 2 cut(s) 343, 743
MwoI GCNNNNNNNGC 3 cut(s) 28, 228, 629
NdeI CATATG 1 cut(s) 550
NdeII GATC 4 cut(s) 36, 346, 553, 645
NheI GCTAGC 1 cut(s) 113
NlaIII CATG 3 cut(s) 395, 580, 733
NlaIV GGNNCC 2 cut(s) 162, 348
NmeAIII GCCGAG 1 cut(s) 51
PagI TCATGA 1 cut(s) 729
PasI CCCWGGG 1 cut(s) 342
PfeI GAWTC 2 cut(s) 305, 337
PflFI GACNNNGTC 1 cut(s) 398
PfoI TCCNGGA 1 cut(s) 741
PkrI GCNGC 1 cut(s) 24
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 233
Psp5II RGGWCCY 1 cut(s) 161
Psp6I CCWGG 2 cut(s) 341, 741
PspGI CCWGG 2 cut(s) 341, 741
PspN4I GGNNCC 2 cut(s) 162, 348
PspPI GGNCC 1 cut(s) 161
PspPPI RGGWCCY 1 cut(s) 161
PsuI RGATCY 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 398
RsaI GTAC 3 cut(s) 245, 654, 752
RsaNI GTAC 3 cut(s) 244, 653, 751
SacI GAGCTC 1 cut(s) 233
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 1 cut(s) 23
Sau3AI GATC 4 cut(s) 36, 346, 553, 645
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 2 cut(s) 343, 743
SduI GDGCHC 1 cut(s) 233
SfaNI GCATC 3 cut(s) 40, 59, 555
SfcI CTRYAG 1 cut(s) 144
SinI GGWCC 1 cut(s) 161
Sse9I AATT 6 cut(s) 183, 205, 236, 289, 310, 356
SsiI CCGC 1 cut(s) 22
SspMI CTAG 3 cut(s) 114, 572, 629
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 2 cut(s) 341, 741
TaaI ACNGT 4 cut(s) 104, 256, 400, 457
TaiI ACGT 1 cut(s) 514
TaqI TCGA 5 cut(s) 8, 193, 233, 402, 648
TasI AATT 6 cut(s) 183, 205, 236, 289, 310, 356
TatI WGTACW 1 cut(s) 750
TauI GCSGC 1 cut(s) 25
TfiI GAWTC 2 cut(s) 305, 337
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TscAI CASTG 1 cut(s) 224
TspDTI ATGAA 3 cut(s) 297, 306, 746
TspGWI ACGGA 2 cut(s) 29, 184
TspRI CASTG 1 cut(s) 224
Tth111I GACNNNGTC 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 161
XagI CCTNNNNNAGG 2 cut(s) 427, 740
XspI CTAG 3 cut(s) 114, 572, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.