Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0003029

Physical Location & Seq

Genomic Coordinates Forward (+)

59335202 .. 59342928

Gene Structure 7727 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG363400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

ATGGCTTCCGCTTCTTCCATGTCAGTTCCTTTAGAATGCGTGAACGTATGTAAGCTGTCGAAAGGGGAAGGGAGTGGGAAATATGACTGTAGCATGTTCTCTTGTGCGTGGAAAGCTCCGAGAGCCTTGACGGGGTTCCTTGCTAGCACAGCTCATTCTTCTCAGTGTTCTTGGCTGTCGTATGGACGAAATGGGAGAAGAAACCGGATGAATTATAAATGTGAATCCTCTAATGTGGGAGGTTGTCATTCTACTGAGGCTTCAGACTTTGTACTTCTTGGACAACTCTATGAATCAGGTTTAGTTCATGTTGCTGGTAAGAGGTGGCAGCTGCACTGTTCTTCTTCCTTGTCCTCAGAAACCACTTATGAGGTTTCTCCTGAAGGATTGTGGGAGGATCTTAAACCAACTATCTCATACCTCTCACCTGAAGAACTAGAACTTGTTCATAATGCTCTTAAGCTGGCTTTTGAGGCCCATGATGGTCAAAAGAGACGCAGCGGAGAGCCATTCATCATTCATCCTGTTGAAGTTGCACGTATTTTAGGGGAACTTGAATTGGATTGGGAGTCCATTGCTTCTGGTTTACTACATGATACAGTCGAAGATACCAATGTGGTTACATTTGAAAGGATAGAGGAGGAGTTTGGTGCTACTGTTCGCCATATTGTAGAAGGAGAGACTAAGGTTTCAAAGTTGGGAAAGTTGAAATGTAAGGATGAACATGATTCTGTACAAGATGTAAAAGCAGATGACCTGCGGCAGATGCTTCTAGCCATGACAGAGGAGGTCCGGGTTATCATTGTCAAGTTAGCTGACAGATTACATAACATGCGCACTCTTTCACACATGCCTCCACATAAGCAGTCCAGCATTGCAAGGGAGACATTGCAGGTCTTTGCTCCTCTAGCAAAGTTGCTTGGGATGTACCAAATCAAGTTGGAACTTGAAAACCTTTCCTTCATGTACACAAATCCTGAAGATTATGCAAAGATCAGGAGAAAAGTGGCAGACCTATACAAAGAGCATGAGAAAGAACTTGTAGAGGCCAACAAAATTTTGGTGAAGAAGATTGAGGATGACCAGTTCTTAGAACTTATGACTGTGAAAACTGAAGTTCGTGCTGTATGCAAGGAACCATACAGTATCTATAAATCTGTTCTCAAATCTAAAGGTTCAATTAATGAGGTCAATCAAATAGCACAGCTTCGTATTGTTATCAAACCAAAGCCATGCCTTGGCGGAGGGCCTCTGTGCACCCCACAGCAGATTTGCTACCATGTTCTTGGGTTGGTTCATGGGATCTGGACCCCTATTCCTCGAACTATGAAAGACTATATTGCCACCCCCAAACCTAATGGCTATCAGAGTCTCCAGACAACTGTAATTCCTTTCTTGTATGAGAGCATGTTGCGGCTTGAAGTGCAGATAAGGACAGAAGAGATGGACCTGATAGCCCAAAGAGGCATTGCTTCCCATTATAGCGGGAGAGGATTTGTTACTGGTTTAGTTGGACGCACAATCCCTAGTGGTAGAAGTTCAAGAGGGAAGACTGTCTGCCTTAACAATGCAAACATTGCACTCAGAATTGGCTGGCTCAATGCAATTAGAGAATGGCAAGAGGAGTTCGTTGGCAACATGAGCTCTAGGGAATTTGTAGAAACCATCACTAGAGATCTATTAGGTAGTCGTGTGTTTGTGTTTACTCCAAGGGGAGAGATAAAAAATCTTCCAAAAGGAGCGACTGTTATTGACTACGCCTATATGATACACACTGAAATTGGCAATAAAATGGTAGCAGCAAAGGTGAACGGTAATCTTGTTTCTCCTATGCATGTACTTGCAAATGCGGAAGTTGTGGAGATAGTCACTTACAATTCACTGACCGGAAAATCAGCTTTTCAGAGGCATAAGCAGTGGTTGCATCATGCAAAAACACGCAGTGCCCGACACAAAATAATGAAATTCTTAAGGGAGCAAGCTGCCCTATCTGCTGATGAAATAACAGCAGATAAAGTAAATGACTTCGTTGCTGATTCTGAAGAAGAGAGTGAAGCAGAAGAGCTCCCCAGTACCTCCAAAGGATACAAAGCTTTGTGGGAGAAAATGCTGGTGAATGTTGTAGGATTGGCATTGCCAGAACGAAGTTCCGAAGATCCCTTCCAAATTACCAATGGAAATGCTCGGTTTTCTGCTCCCAAGGTGAACGGGAAACATAACAAACATGTTCGACATGTGAGTTTGAAGGCTGAGGGGGAGTTGTTGTCACAAGGAAATGGTGTTGCTAGAATGTTGCAAGCTAATATTCCTATGTACAAGGAAGCCTTGCCCAGTTTAGAAAGTTGGCAAGCCAGCAAGATTACCTCTTGGCATAATGTTGAAGGGCACTCCATCCTTTGGTTTTCCGTGGTTTCCATCGATCGAAAAGGTATGATCGCTGAGGTCACAACAGCAATGGCAGCTGCAGGCATTACTATTTGTTCTTGTGTGGCTGAGATCGATGGGGAAAGGGGGATCGCTGTCATGTTATTTCACGTTGAAGGGAGCGTGGAAAATTTGGCTAATGCTTGCTCGAGCATTGATATTATTCTTGGTGTTTTGGGATGGTCGATGGGTTGCAGCTGGCCCAGCTTAATGGATAAGCCCCGATTACTTGAATGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008893 GO:0009507 GO:0009536 GO:0009611 GO:0009987 GO:0010150 GO:0015979 GO:0016787 GO:0016788 GO:0016794 GO:0032501 GO:0032502 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0090693 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00230 map00230

Pfam Domains

Protein Families

HD_4 RelA_SpoT TGS

Protein Analysis

887

Amino Acids

98.84

Weight (kDa)

6.76

Isoelectric Point (pI)

44.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HD_4	PF13328	152 - 309	8.9e-45	HD domain
HD	PF01966	172 - 278	6.2e-12	HD domain
RelA_SpoT	PF04607	378 - 494	1.7e-22	Region found in RelA / SpoT proteins
TGS	PF02824	565 - 624	4.9e-21	TGS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02260 AT4G02260 AT4G02260 AT4G02260
fragaria_vesca	FvH4_2g26030 FvH4_2g26030 FvH4_3g31250
malus_domestica	MD03G1291500.v1.1 MD11G1306700.v1.1
prunus_persica	Prupe.8G264600_v2.0.a1
pyrus_communis	pycom03g23090 pycom11g26840
rosa_chinensis	RchiOBHm_Chr6g0294391
rosa_laevigata	RLG00000011874
rosa_multiflora	Rmu_co8168868.1_g000001 Rmu_sc0000276.1_g000002 Rmu_sc0024449.1_g000001 Rmu_sc0030396.1_g000001
rosa_roxburghii	Rroxscaffold_4G00298810 Rroxscaffold_7G00173410
rosa_rugosa	Rorug04G0066700 Rorug04G0066800 Rorug04G0066900 Rorug04G0066900 Rorug04G0067000 Rorug04G0067100 Rorug06G0244100 Rorug06G0244200
rosa_samantha	Rh4AG095600 Rh6AG356700 Rh6BG363400 Rh6CG370700 Rh6DG357400 Rh7BG000400
rosa_wichuraiana	Rw4G020730 Rw6G017020 Rw6G031090 Rw7G015830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	216
Acc16I	TGCGCA	1 cut(s)	836
Acc36I	ACCTGC	2 cut(s)	765, 883
AccB7I	CCANNNNNTGG	2 cut(s)	1238, 2397
AciI	CCGC	7 cut(s)	9, 501, 760, 1242, 1414, 1485, 1850
AclWI	GGATC	4 cut(s)	405, 1310, 2150, 2522
AcsI	RAATTY	4 cut(s)	1056, 1652, 1964, 2554
AcuI	CTGAAG	6 cut(s)	246, 402, 450, 999, 1134, 2061
AdeI	CACNNNGTG	1 cut(s)	1943
AfaI	GTAC	7 cut(s)	273, 735, 929, 968, 1839, 2074, 2315
AfiI	CCNNNNNNNGG	3 cut(s)	132, 1238, 2397
AflII	CTTAAG	2 cut(s)	458, 1969
AflIII	ACRYGT	2 cut(s)	2224, 2233
AloI	GAACNNNNNNTCC	2 cut(s)	1070, 1102
Alw21I	GWGCWC	3 cut(s)	1259, 1646, 2067
Alw26I	GTCTC	4 cut(s)	487, 674, 878, 1376
Alw44I	GTGCAC	1 cut(s)	1255
AlwI	GGATC	4 cut(s)	405, 1310, 2150, 2522
Ama87I	CYCGRG	1 cut(s)	2572
AoxI	GGCC	4 cut(s)	474, 1047, 1247, 2624
ApaLI	GTGCAC	1 cut(s)	1255
ApeKI	GCWGC	8 cut(s)	328, 331, 498, 1799, 1982, 2459, 2462, 2619
ApoI	RAATTY	4 cut(s)	1056, 1652, 1964, 2554
AseI	ATTAAT	1 cut(s)	1182
Asp700I	GAANNNNTTC	1 cut(s)	444
AspLEI	GCGC	1 cut(s)	837
AspS9I	GGNCC	6 cut(s)	475, 790, 1247, 1308, 1447, 2625
AsuC2I	CCSGG	1 cut(s)	794
AsuHPI	GGTGA	5 cut(s)	417, 1075, 1819, 2125, 2215
AsuNHI	GCTAGC	1 cut(s)	143
AvaI	CYCGRG	1 cut(s)	2572
AvaII	GGWCC	3 cut(s)	790, 1308, 1447
BaeGI	GKGCMC	3 cut(s)	1259, 1948, 2388
BaeI	ACNNNNGTAYC	2 cut(s)	600, 633
BanII	GRGCYC	2 cut(s)	1646, 2067
BbsI	GAAGAC	1 cut(s)	1556
Bbv12I	GWGCWC	3 cut(s)	1259, 1646, 2067
BbvCI	CCTCAGC	2 cut(s)	2250, 2439
BbvI	GCAGC	8 cut(s)	318, 340, 510, 1811, 1969, 2449, 2471, 2631
BccI	CCATC	8 cut(s)	476, 1438, 1673, 2399, 2423, 2495, 2598, 2605
BciVI	GTATCC	1 cut(s)	2078
BcnI	CCSGG	1 cut(s)	794
BcoDI	GTCTC	4 cut(s)	487, 674, 878, 1376
BfaI	CTAG	8 cut(s)	144, 437, 773, 908, 1527, 1647, 1671, 2286
BfmI	CTRYAG	2 cut(s)	88, 2463
BfrI	CTTAAG	2 cut(s)	458, 1969
BfuAI	ACCTGC	2 cut(s)	765, 883
BfuI	GTATCC	1 cut(s)	2078
BglII	AGATCT	1 cut(s)	1675
Bme1390I	CCNGG	1 cut(s)	794
Bme18I	GGWCC	3 cut(s)	790, 1308, 1447
BmeT110I	CYCGRG	1 cut(s)	2572
BmgT120I	GGNCC	6 cut(s)	475, 790, 1247, 1308, 1447, 2625
BmiI	GGNNCC	3 cut(s)	137, 1137, 1310
BmrFI	CCNGG	1 cut(s)	794
BmrI	ACTGGG	2 cut(s)	2064, 2325
BmsI	GCATC	2 cut(s)	756, 1933
BmtI	GCTAGC	1 cut(s)	147
BmuI	ACTGGG	2 cut(s)	2064, 2325
BpiI	GAAGAC	1 cut(s)	1556
BpmI	CTGGAG	1 cut(s)	1358
Bpu10I	CCTNAGC	2 cut(s)	2250, 2439
BpuMI	CCSGG	1 cut(s)	794
Bsa29I	ATCGAT	2 cut(s)	2418, 2499
BsaAI	YACGTR	1 cut(s)	539
BsaJI	CCNNGG	4 cut(s)	1237, 1709, 2199, 2406
BsaWI	WCCGGW	2 cut(s)	204, 1886
BsaXI	ACNNNNNCTCC	4 cut(s)	1731, 1761, 2249, 2279
Bsc4I	CCNNNNNNNGG	3 cut(s)	132, 1238, 2397
Bse1I	ACTGG	4 cut(s)	1084, 1507, 2070, 2331
Bse3DI	GCAATG	7 cut(s)	573, 873, 887, 1467, 1575, 2132, 2460
BseCI	ATCGAT	2 cut(s)	2418, 2499
BseDI	CCNNGG	4 cut(s)	1237, 1709, 2199, 2406
BseGI	GGATG	7 cut(s)	213, 520, 724, 930, 1084, 2391, 2609
BseLI	CCNNNNNNNGG	3 cut(s)	132, 1238, 2397
BseMI	GCAATG	7 cut(s)	573, 873, 887, 1467, 1575, 2132, 2460
BseMII	CTCAG	7 cut(s)	176, 246, 369, 1597, 2241, 2430, 2484
BseNI	ACTGG	4 cut(s)	1084, 1507, 2070, 2331
BseRI	GAGGAG	5 cut(s)	653, 656, 800, 894, 1637
BseSI	GKGCMC	3 cut(s)	1259, 1948, 2388
BseXI	GCAGC	8 cut(s)	318, 340, 510, 1811, 1969, 2449, 2471, 2631
BseYI	CCCAGC	1 cut(s)	2627
BsgI	GTGCAG	2 cut(s)	317, 1445
Bsh1285I	CGRYCG	1 cut(s)	2422
BshFI	GGCC	4 cut(s)	476, 1049, 1249, 2626
BshVI	ATCGAT	2 cut(s)	2418, 2499
BsiEI	CGRYCG	1 cut(s)	2422
BsiHKAI	GWGCWC	3 cut(s)	1259, 1646, 2067
BsiHKCI	CYCGRG	1 cut(s)	2572
BsiSI	CCGG	3 cut(s)	205, 793, 1887
BslI	CCNNNNNNNGG	3 cut(s)	132, 1238, 2397
BsmAI	GTCTC	4 cut(s)	487, 674, 878, 1376
BsmBI	CGTCTC	1 cut(s)	487
BsmI	GAATGC	2 cut(s)	41, 2663
BsnI	GGCC	4 cut(s)	476, 1049, 1249, 2626
BsoBI	CYCGRG	1 cut(s)	2572
Bsp1286I	GDGCHC	5 cut(s)	1259, 1646, 1948, 2067, 2388
Bsp1407I	TGTACA	3 cut(s)	733, 966, 2313
Bsp143I	GATC	9 cut(s)	397, 993, 1302, 1675, 2155, 2419, 2433, 2496, 2514
BspACI	CCGC	7 cut(s)	9, 501, 760, 1242, 1414, 1485, 1850
BspANI	GGCC	4 cut(s)	476, 1049, 1249, 2626
BspCNI	CTCAG	7 cut(s)	175, 247, 368, 1596, 2242, 2431, 2485
BspDI	ATCGAT	2 cut(s)	2418, 2499
BspLI	GGNNCC	3 cut(s)	137, 1137, 1310
BspMAI	CTGCAG	1 cut(s)	2467
BspMI	ACCTGC	2 cut(s)	765, 883
BspOI	GCTAGC	1 cut(s)	147
BspPI	GGATC	4 cut(s)	405, 1310, 2150, 2522
BspQI	GCTCTTC	1 cut(s)	2055
BspTI	CTTAAG	2 cut(s)	458, 1969
BsrDI	GCAATG	7 cut(s)	573, 873, 887, 1467, 1575, 2132, 2460
BsrGI	TGTACA	3 cut(s)	733, 966, 2313
BsrI	ACTGG	4 cut(s)	1084, 1507, 2070, 2331
BssECI	CCNNGG	4 cut(s)	1237, 1709, 2199, 2406
BssMI	GATC	9 cut(s)	397, 993, 1302, 1675, 2155, 2419, 2433, 2496, 2514
BssT1I	CCWWGG	3 cut(s)	1237, 1709, 2199
Bst6I	CTCTTC	3 cut(s)	1434, 2040, 2055
BstAFI	CTTAAG	2 cut(s)	458, 1969
BstAPI	GCANNNNNTGC	2 cut(s)	1577, 1921
BstAUI	TGTACA	3 cut(s)	733, 966, 2313
BstBAI	YACGTR	1 cut(s)	539
BstDEI	CTNAG	9 cut(s)	162, 255, 355, 684, 1090, 1583, 2250, 2439, 2493
BstDSI	CCRYGG	1 cut(s)	2406
BstF5I	GGATG	7 cut(s)	213, 520, 724, 930, 1084, 2391, 2609
BstHHI	GCGC	1 cut(s)	837
BstKTI	GATC	9 cut(s)	400, 996, 1305, 1678, 2158, 2422, 2436, 2499, 2517
BstMAI	GTCTC	4 cut(s)	487, 674, 878, 1376
BstMBI	GATC	9 cut(s)	397, 993, 1302, 1675, 2155, 2419, 2433, 2496, 2514
BstMCI	CGRYCG	1 cut(s)	2422
BstNSI	RCATGY	7 cut(s)	97, 835, 853, 1411, 1838, 2228, 2237
BstSCI	CCNGG	1 cut(s)	792
BstSFI	CTRYAG	2 cut(s)	88, 2463
BstSLI	GKGCMC	3 cut(s)	1259, 1948, 2388
BstV1I	GCAGC	8 cut(s)	318, 340, 510, 1811, 1969, 2449, 2471, 2631
BstV2I	GAAGAC	1 cut(s)	1556
BstX2I	RGATCY	4 cut(s)	397, 1302, 1675, 2155
BstXI	CCANNNNNNTGG	2 cut(s)	1286, 2635
BstYI	RGATCY	4 cut(s)	397, 1302, 1675, 2155
Bsu15I	ATCGAT	2 cut(s)	2418, 2499
BsuI	GTATCC	1 cut(s)	2078
BsuRI	GGCC	4 cut(s)	476, 1049, 1249, 2626
BsuTUI	ATCGAT	2 cut(s)	2418, 2499
BtgI	CCRYGG	1 cut(s)	2406
BtsCI	GGATG	7 cut(s)	213, 520, 724, 930, 1084, 2391, 2609
BtsI	GCAGTG	2 cut(s)	1922, 1948
BtsIMutI	CAGTG	6 cut(s)	170, 334, 1773, 1880, 1922, 1948
BveI	ACCTGC	2 cut(s)	765, 883
CfoI	GCGC	1 cut(s)	837
Cfr13I	GGNCC	6 cut(s)	475, 790, 1247, 1308, 1447, 2625
ClaI	ATCGAT	2 cut(s)	2418, 2499
CseI	GACGC	2 cut(s)	504, 1524
Csp6I	GTAC	7 cut(s)	272, 734, 928, 967, 1838, 2073, 2314
CviQI	GTAC	7 cut(s)	272, 734, 928, 967, 1838, 2073, 2314
DdeI	CTNAG	9 cut(s)	162, 255, 355, 684, 1090, 1583, 2250, 2439, 2493
DpnI	GATC	9 cut(s)	399, 995, 1304, 1677, 2157, 2421, 2435, 2498, 2516
DpnII	GATC	9 cut(s)	397, 993, 1302, 1675, 2155, 2419, 2433, 2496, 2514
DraIII	CACNNNGTG	1 cut(s)	1943
Eam1104I	CTCTTC	3 cut(s)	1434, 2040, 2055
EarI	CTCTTC	3 cut(s)	1434, 2040, 2055
EciI	GGCGGA	1 cut(s)	1257
Ecl136II	GAGCTC	2 cut(s)	1644, 2065
Eco130I	CCWWGG	3 cut(s)	1237, 1709, 2199
Eco24I	GRGCYC	2 cut(s)	1646, 2067
Eco47I	GGWCC	3 cut(s)	790, 1308, 1447
Eco53kI	GAGCTC	2 cut(s)	1644, 2065
Eco57I	CTGAAG	6 cut(s)	246, 402, 450, 999, 1134, 2061
Eco88I	CYCGRG	1 cut(s)	2572
EcoICRI	GAGCTC	2 cut(s)	1644, 2065
EcoO109I	RGGNCCY	1 cut(s)	1247
EcoT14I	CCWWGG	3 cut(s)	1237, 1709, 2199
EcoT22I	ATGCAT	1 cut(s)	1836
EcoT38I	GRGCYC	2 cut(s)	1646, 2067
ErhI	CCWWGG	3 cut(s)	1237, 1709, 2199
Esp3I	CGTCTC	1 cut(s)	487
FalI	AAGNNNNNCTT	2 cut(s)	2309, 2341
FauI	CCCGC	1 cut(s)	1478
FokI	GGATG	7 cut(s)	220, 507, 731, 937, 1091, 2378, 2616
FriOI	GRGCYC	2 cut(s)	1646, 2067
FspAI	RTGCGCAY	1 cut(s)	836
FspBI	CTAG	8 cut(s)	144, 437, 773, 908, 1527, 1647, 1671, 2286
FspI	TGCGCA	1 cut(s)	836
GlaI	GCGC	1 cut(s)	836
GsaI	CCCAGC	1 cut(s)	2631
GsuI	CTGGAG	1 cut(s)	1358
HaeIII	GGCC	4 cut(s)	476, 1049, 1249, 2626
HapII	CCGG	3 cut(s)	205, 793, 1887
HgaI	GACGC	2 cut(s)	504, 1524
HhaI	GCGC	1 cut(s)	837
Hin6I	GCGC	1 cut(s)	835
HinP1I	GCGC	1 cut(s)	835
HindIII	AAGCTT	1 cut(s)	2091
HinfI	GANTC	6 cut(s)	224, 293, 569, 728, 1369, 2036
HpaII	CCGG	3 cut(s)	205, 793, 1887
HphI	GGTGA	5 cut(s)	417, 1075, 1819, 2125, 2215
Hpy166II	GTNNAC	7 cut(s)	43, 587, 969, 1257, 1704, 1810, 2206
Hpy188I	TCNGA	8 cut(s)	120, 265, 358, 1368, 1586, 1905, 2041, 2152
Hpy188III	TCNNGA	6 cut(s)	380, 977, 997, 1306, 1375, 1542
Hpy8I	GTNNAC	7 cut(s)	43, 587, 969, 1257, 1704, 1810, 2206
HpyAV	CCTTC	8 cut(s)	62, 377, 668, 970, 2170, 2239, 2375, 2534
HpyCH4IV	ACGT	3 cut(s)	45, 538, 2535
HpyF3I	CTNAG	9 cut(s)	162, 255, 355, 684, 1090, 1583, 2250, 2439, 2493
HpySE526I	ACGT	3 cut(s)	45, 538, 2535
HspAI	GCGC	1 cut(s)	835
Kzo9I	GATC	9 cut(s)	397, 993, 1302, 1675, 2155, 2419, 2433, 2496, 2514
LguI	GCTCTTC	1 cut(s)	2055
LmnI	GCTCC	7 cut(s)	121, 907, 1739, 1975, 2070, 2200, 2544
Lsp1109I	GCAGC	8 cut(s)	318, 340, 510, 1811, 1969, 2449, 2471, 2631
LweI	GCATC	2 cut(s)	756, 1933
MaeI	CTAG	8 cut(s)	144, 437, 773, 908, 1527, 1647, 1671, 2286
MaeII	ACGT	3 cut(s)	45, 538, 2535
MaeIII	GTNAC	5 cut(s)	619, 1498, 1867, 2265, 2443
MalI	GATC	9 cut(s)	399, 995, 1304, 1677, 2157, 2421, 2435, 2498, 2516
MboI	GATC	9 cut(s)	397, 993, 1302, 1675, 2155, 2419, 2433, 2496, 2514
MflI	RGATCY	4 cut(s)	397, 1302, 1675, 2155
MhlI	GDGCHC	5 cut(s)	1259, 1646, 1948, 2067, 2388
MlyI	GAGTC	2 cut(s)	578, 1378
MmeI	TCCRAC	2 cut(s)	921, 1492
Mph1103I	ATGCAT	1 cut(s)	1836
MroXI	GAANNNNTTC	1 cut(s)	444
MseI	TTAA	6 cut(s)	402, 459, 1182, 1563, 1970, 2633
MspA1I	CMGCKG	4 cut(s)	331, 501, 2462, 2622
MspCI	CTTAAG	2 cut(s)	458, 1969
MspI	CCGG	3 cut(s)	205, 793, 1887
MspR9I	CCNGG	1 cut(s)	794
Mva1269I	GAATGC	2 cut(s)	41, 2663
NciI	CCSGG	1 cut(s)	794
NdeII	GATC	9 cut(s)	397, 993, 1302, 1675, 2155, 2419, 2433, 2496, 2514
NheI	GCTAGC	1 cut(s)	143
NlaIV	GGNNCC	3 cut(s)	137, 1137, 1310
NmuCI	GTSAC	3 cut(s)	1867, 2265, 2443
NsbI	TGCGCA	1 cut(s)	836
NsiI	ATGCAT	1 cut(s)	1836
NspI	RCATGY	7 cut(s)	97, 835, 853, 1411, 1838, 2228, 2237
PaeR7I	CTCGAG	1 cut(s)	2572
PciI	ACATGT	2 cut(s)	2224, 2233
PciSI	GCTCTTC	1 cut(s)	2055
PcsI	WCGNNNNNNNCGW	1 cut(s)	1945
PctI	GAATGC	2 cut(s)	41, 2663
PdmI	GAANNNNTTC	1 cut(s)	444
PfeI	GAWTC	4 cut(s)	224, 293, 728, 2036
PflMI	CCANNNNNTGG	2 cut(s)	1238, 2397
Ple19I	CGATCG	1 cut(s)	2422
PleI	GAGTC	2 cut(s)	577, 1377
PpsI	GAGTC	2 cut(s)	577, 1377
Ppu21I	YACGTR	1 cut(s)	539
PscI	ACATGT	2 cut(s)	2224, 2233
PshBI	ATTAAT	1 cut(s)	1182
PsiI	TTATAA	1 cut(s)	216
Psp124BI	GAGCTC	2 cut(s)	1646, 2067
PspFI	CCCAGC	1 cut(s)	2627
PspN4I	GGNNCC	3 cut(s)	137, 1137, 1310
PspPI	GGNCC	6 cut(s)	475, 790, 1247, 1308, 1447, 2625
PspXI	VCTCGAGB	1 cut(s)	2572
PstI	CTGCAG	1 cut(s)	2467
PsuI	RGATCY	4 cut(s)	397, 1302, 1675, 2155
PvuI	CGATCG	1 cut(s)	2422
PvuII	CAGCTG	3 cut(s)	331, 2462, 2622
RsaI	GTAC	7 cut(s)	273, 735, 929, 968, 1839, 2074, 2315
RsaNI	GTAC	7 cut(s)	272, 734, 928, 967, 1838, 2073, 2314
SacI	GAGCTC	2 cut(s)	1646, 2067
SapI	GCTCTTC	1 cut(s)	2055
SaqAI	TTAA	6 cut(s)	402, 459, 1182, 1563, 1970, 2633
Sau3AI	GATC	9 cut(s)	397, 993, 1302, 1675, 2155, 2419, 2433, 2496, 2514
Sau96I	GGNCC	6 cut(s)	475, 790, 1247, 1308, 1447, 2625
SchI	GAGTC	2 cut(s)	578, 1378
ScrFI	CCNGG	1 cut(s)	794
SduI	GDGCHC	5 cut(s)	1259, 1646, 1948, 2067, 2388
SfaNI	GCATC	2 cut(s)	756, 1933
SfcI	CTRYAG	2 cut(s)	88, 2463
Sfr274I	CTCGAG	1 cut(s)	2572
SinI	GGWCC	3 cut(s)	790, 1308, 1447
SlaI	CTCGAG	1 cut(s)	2572
SmlI	CTYRAG	3 cut(s)	458, 1969, 2572
SmoI	CTYRAG	3 cut(s)	458, 1969, 2572
SsiI	CCGC	7 cut(s)	9, 501, 760, 1242, 1414, 1485, 1850
SspI	AATATT	1 cut(s)	2305
SspMI	CTAG	8 cut(s)	144, 437, 773, 908, 1527, 1647, 1671, 2286
SstI	GAGCTC	2 cut(s)	1646, 2067
StyD4I	CCNGG	1 cut(s)	792
StyI	CCWWGG	3 cut(s)	1237, 1709, 2199
TaiI	ACGT	3 cut(s)	48, 541, 2538
TaqI	TCGA	9 cut(s)	59, 603, 1321, 2230, 2418, 2422, 2499, 2573, 2609
TatI	WGTACW	5 cut(s)	271, 733, 966, 1837, 2313
TauI	GCSGC	2 cut(s)	763, 1417
TfiI	GAWTC	4 cut(s)	224, 293, 728, 2036
Tru1I	TTAA	6 cut(s)	402, 459, 1182, 1563, 1970, 2633
Tru9I	TTAA	6 cut(s)	402, 459, 1182, 1563, 1970, 2633
TscAI	CASTG	6 cut(s)	170, 341, 1780, 1887, 1922, 1948
TseFI	GTSAC	3 cut(s)	1867, 2265, 2443
TseI	GCWGC	8 cut(s)	328, 331, 498, 1799, 1982, 2459, 2462, 2619
Tsp45I	GTSAC	3 cut(s)	1867, 2265, 2443
TspGWI	ACGGA	1 cut(s)	2395
TspRI	CASTG	6 cut(s)	170, 341, 1780, 1887, 1922, 1948
Van91I	CCANNNNNTGG	2 cut(s)	1238, 2397
Vha464I	CTTAAG	2 cut(s)	458, 1969
VneI	GTGCAC	1 cut(s)	1255
VpaK11BI	GGWCC	3 cut(s)	790, 1308, 1447
VspI	ATTAAT	1 cut(s)	1182
XapI	RAATTY	4 cut(s)	1056, 1652, 1964, 2554
XceI	RCATGY	7 cut(s)	97, 835, 853, 1411, 1838, 2228, 2237
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1057, 2171
XhoI	CTCGAG	1 cut(s)	2572
XmnI	GAANNNNTTC	1 cut(s)	444
XspI	CTAG	8 cut(s)	144, 437, 773, 908, 1527, 1647, 1671, 2286
Zsp2I	ATGCAT	1 cut(s)	1836

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.