Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003029

Physical Location & Seq

Genomic Coordinates Forward (+)

36900241 .. 36907843

Gene Structure 7603 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1291500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGTCTTCCGCTCCTTCCATGTCAGTTTCTTTAGAATGCGTGAATGTGTGTAAGCTATCGAAAGGGGACGGAAGTGGGAGAAATGACTGTAGCGTGTTCTCTTGTGCGTGGAAAGCTCCAAGAGTCTTGACGGGGTTTCTTGCGAGCACGGCTCATCATCCTCAATGTTCGTGGTTGCCAGATGCACGAAATGGAAGAAGGAACCGGACTAATCATAGATATCAGTCCTCTAGCGTAGGAGGTTGGAATTCTGCTGAAGCTTCAGATTTTGTAGTTCTAGGAAGACTGCTTAAATCAGGTTTTCTTTTTGTTGGTGGTAAGAGATGGCATCTGCGATGTTCTTCATCTTTGTCTTCAGCTGTCTTGGATGATATTTCTTCTGAAACATTGTGGGAGGATCTTAAGCCGTCTATCTCATACCTCTCACCTAAAGAACTGGAACTTGTCCATAATGCTCTTAAGCTGGCTTTTGACGCACATGATGGTCAGAAAAGGCGCAGTGGAGAGCCTTTCATCATTCATCCAGTTGAAGTTGCACGAATTTTAGGAGAACTCGAGTTGGATTGGGTATCCATTGCTTCTGGATTACTACATGATACAGTCGAGGATACCAATGTTGTTACTTTCGAAAGGATAGAGGAGGAGTTTGGCGCTACTGTTCGCCATATTGTAGAAGGAGAAACTAAGGTGTCAAAGCTGGGAAAATTGAAGTGCAAGAGTGAAGAGGATTCTGTACAAGATGTAAAAGCAGATGACCTTCGCCAGATGCTTCTAGCCATGACAGAGGAGGTCCGAGTTATCATCGTCAAATTAGCTGACAGACTCCACAACATGCGTACCCTTTCACACATGCCACCGCATAAGCAGTCAAGCATTGCACAGGAGACATTGCAGGTCTTTGCTCCTCTAGCAAAGTTGCTTGGGATGTACCAAATCAAGTCGGAACTTGAAAACCTTTCCTTCATGTACACAAATGCTGAAGATTATGCTAAGATCAAGAGAAGAGTTGCAGGCCTCTACAAAGAGCATGAGAAAGAACTTGTGGAGGCAAACAAAATTTTGTTGAAGAGGATTCAGGATGATGAGTTCTTAGAACTTATGGAGGTGAAAACTGAAGTTCGTGCCGTGTGTAAGGAACCATACAGTATCTACAAGGCCGCGCTCAAATGTAAAAGTTCAATTAACGAGATCAATCAAATTGCACAGCTTCGAATTATAATCAAACCAAAGCCATCCCTTGGAGCTGGACCTTTGTGCACCCCACAACAGATCTGCTACCATGTTCTTGGGCTGGTTCATGGAATCTGGACCCCTATTCCTCGGATGATGAAAGACTATATTGCCACCCCAAAACCTAATGGCTATCAGAGTCTCCATACAACTGTAATTCCTTTCTTGTATGAAAGCATGTTGCGGCTAGAAGTGCAGATAAGAACAGAAGAGATGGATCTGATAGCTCAGAGAGGAATTGCTTCTCATTATAGTGGGAGAGGATTTGTTACTGTTTTAATTGGGCGTAGTCTCCCTAATGGTAGAAGTTCAAGAGGGAAGACTGTTTGCCTTAACAATGCAAACATTGCGCTCAGGATTGGCTGGCTCAATGCAATTAGAGAATGGCAAGAAGAATTTGTTGGCAACATGAGCTCTAGAGAATTTGTTGAAACTATCACTAGAGATCTATTAGGTAGTCGTGTGTTTGTGTTTACTCCCAGGGGAGAGATAAAAAATCTTCCTAAAGGAGCAACTGTTATTGACTATGCATACATGATACACACTGAAATTGGCAACAAAATGGTAGCAGCAAAGGTCAATGGAAATCTTGTTTCTCCTATGCATGTACTTGTAAATGCAGAAGTTGTGGAGATAATCACTTATAATTCACTTACCGGAAAATCAGCTTTTCAGAGGCACAAACAGTGGTTGCAACATGCAAAAACACGTAGTGCCAGGCACAAAATTATGAAATTCTTAAGGGAGCAAGCAGCCCTGTCTGTTGCTGAAATAACAGCCGATAAAGTAAATGACTTCATTGCTGATTCTGAAGAAGAGATTGAAGCAGAAGAGCTCCCAAGTACCTTCAAAGGATACAAACCTATATGGGAAAAAATGATGGGGAATGTTGTAGAAGTTTCATTGCCAGAACGAAGTTCTATAGATCCGTTTCAAATTACAAATGGAAGCGCCTTGGCTCCCAAGGTGAATGGGAAGCACAACAAGAATGTTCAGCATGTAAGTTTAAAGGCTGCGGGGGAGTCCTTGTCGCAAGGGAATGGCATTGCCAAAATGTTGCAAGCTAACATTCCAATGTGCAAGGAAGCCTTGCCCAGTTTAGAAAGTTGGCAGGCCAGCAAGGTTGCATCGTGGCATAGTATTGAAGGGCACTCCATACAATGGTTCTGTGTGGTTTGTGTTGATCGAAAAGGTATGATGGCTGAGGTTACTACAGCATTGGCAGCTGCAGGCATTACAATTTGTTCTTGTGTGGCTGAGATTGACGGGGAAAGGGGAATGGCGGTCATGTTATTTCACGTCGAAGGAAATGTGGAAAGTTTGGTTATTGCATGCTCAAGCATCGATATAATTCTAGGCGTATTGGGATGGTCTACGGGTTGCAGCGTACCTAGCTCAATGGATAATCTGCAATATCTTGAATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008893 GO:0009507 GO:0009536 GO:0009611 GO:0009987 GO:0010150 GO:0015979 GO:0016787 GO:0016788 GO:0016794 GO:0032501 GO:0032502 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0090693 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00230 map00230

Pfam Domains

Protein Families

HD_4 RelA_SpoT TGS

Protein Analysis

886

Amino Acids

98.4

Weight (kDa)

7.33

Isoelectric Point (pI)

47.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HD_4	PF13328	152 - 309	4.9e-44	HD domain
HD	PF01966	172 - 278	2.9e-11	HD domain
RelA_SpoT	PF04607	378 - 494	7.8e-24	Region found in RelA / SpoT proteins
TGS	PF02824	565 - 624	1.7e-20	TGS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02260 AT4G02260 AT4G02260 AT4G02260
fragaria_vesca	FvH4_2g26030 FvH4_2g26030 FvH4_3g31250
malus_domestica	MD03G1291500.v1.1 MD11G1306700.v1.1
prunus_persica	Prupe.8G264600_v2.0.a1
pyrus_communis	pycom03g23090 pycom11g26840
rosa_chinensis	RchiOBHm_Chr6g0294391
rosa_laevigata	RLG00000011874
rosa_multiflora	Rmu_co8168868.1_g000001 Rmu_sc0000276.1_g000002 Rmu_sc0024449.1_g000001 Rmu_sc0030396.1_g000001
rosa_roxburghii	Rroxscaffold_4G00298810 Rroxscaffold_7G00173410
rosa_rugosa	Rorug04G0066700 Rorug04G0066800 Rorug04G0066900 Rorug04G0066900 Rorug04G0067000 Rorug04G0067100 Rorug06G0244100 Rorug06G0244200
rosa_samantha	Rh4AG095600 Rh6AG356700 Rh6BG363400 Rh6CG370700 Rh6DG357400 Rh7BG000400
rosa_wichuraiana	Rw4G020730 Rw6G017020 Rw6G031090 Rw7G015830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1217, 1875
Acc36I	ACCTGC	1 cut(s)	883
AccB7I	CCANNNNNTGG	2 cut(s)	1238, 2391
AccBSI	CCGCTC	1 cut(s)	11
AccI	GTMKAC	1 cut(s)	2603
AccII	CGCG	1 cut(s)	1160
AciI	CCGC	6 cut(s)	9, 857, 1158, 1414, 2246, 2513
AclWI	GGATC	3 cut(s)	405, 1455, 2150
AcsI	RAATTY	6 cut(s)	247, 540, 1056, 1625, 1652, 1964
AcuI	CTGAAG	6 cut(s)	246, 276, 339, 999, 1134, 2061
AdeI	CACNNNGTG	1 cut(s)	1943
AfaI	GTAC	7 cut(s)	735, 838, 929, 968, 1839, 2074, 2619
AfiI	CCNNNNNNNGG	2 cut(s)	1238, 2391
AflII	CTTAAG	3 cut(s)	401, 458, 1969
AflIII	ACRYGT	1 cut(s)	1937
AjiI	CACGTC	1 cut(s)	2530
AjnI	CCWGG	2 cut(s)	1709, 1946
AjuI	GAANNNNNNNTTGG	2 cut(s)	1614, 1646
AloI	GAACNNNNNNTCC	2 cut(s)	1070, 1102
Alw21I	GWGCWC	4 cut(s)	149, 1259, 1646, 2067
Alw26I	GTCTC	3 cut(s)	878, 1376, 1526
Alw44I	GTGCAC	1 cut(s)	1255
AlwI	GGATC	3 cut(s)	405, 1455, 2150
Ama87I	CYCGRG	1 cut(s)	554
AoxI	GGCC	3 cut(s)	1012, 1155, 2343
ApaLI	GTGCAC	1 cut(s)	1255
ApeKI	GCWGC	6 cut(s)	1799, 1982, 2243, 2453, 2456, 2613
ApoI	RAATTY	6 cut(s)	247, 540, 1056, 1625, 1652, 1964
Asp700I	GAANNNNTTC	1 cut(s)	1471
AspLEI	GCGC	5 cut(s)	498, 653, 1162, 1582, 2183
AspS9I	GGNCC	3 cut(s)	790, 1247, 1308
AsuHPI	GGTGA	3 cut(s)	417, 1117, 2209
AsuII	TTCGAA	2 cut(s)	627, 1210
AvaI	CYCGRG	1 cut(s)	554
AvaII	GGWCC	3 cut(s)	790, 1247, 1308
BaeGI	GKGCMC	2 cut(s)	1259, 2382
BaeI	ACNNNNGTAYC	4 cut(s)	600, 633, 820, 853
BanII	GRGCYC	2 cut(s)	1646, 2067
BbsI	GAAGAC	3 cut(s)	289, 345, 1556
Bbv12I	GWGCWC	4 cut(s)	149, 1259, 1646, 2067
BbvCI	CCTCAGC	1 cut(s)	2433
BbvI	GCAGC	6 cut(s)	1811, 1994, 2230, 2443, 2465, 2625
BccI	CCATC	7 cut(s)	318, 476, 1240, 1438, 2104, 2422, 2592
BceAI	ACGGC	3 cut(s)	165, 391, 1109
BciT130I	CCWGG	2 cut(s)	1711, 1948
BciVI	GTATCC	3 cut(s)	580, 601, 2078
BcoDI	GTCTC	3 cut(s)	878, 1376, 1526
BfaI	CTAG	9 cut(s)	231, 278, 773, 908, 1418, 1647, 1671, 2585, 2622
BfmI	CTRYAG	4 cut(s)	88, 2151, 2442, 2457
BfoI	RGCGCY	2 cut(s)	654, 2184
BfrI	CTTAAG	3 cut(s)	401, 458, 1969
BfuAI	ACCTGC	1 cut(s)	883
BfuI	GTATCC	3 cut(s)	580, 601, 2078
BglII	AGATCT	2 cut(s)	1269, 1675
BisI	GCNGC	8 cut(s)	1158, 1415, 1800, 1983, 2244, 2454, 2457, 2614
BlsI	GCNGC	8 cut(s)	1159, 1416, 1801, 1984, 2245, 2455, 2458, 2615
Bme1390I	CCNGG	2 cut(s)	1711, 1948
Bme18I	GGWCC	3 cut(s)	790, 1247, 1308
BmeT110I	CYCGRG	1 cut(s)	554
BmgBI	CACGTC	1 cut(s)	2530
BmgT120I	GGNCC	3 cut(s)	790, 1247, 1308
BmiI	GGNNCC	4 cut(s)	203, 1137, 1310, 2190
BmrFI	CCNGG	2 cut(s)	1711, 1948
BmrI	ACTGGG	1 cut(s)	2319
BmsI	GCATC	5 cut(s)	172, 337, 756, 2366, 2580
BmuI	ACTGGG	1 cut(s)	2319
BpiI	GAAGAC	3 cut(s)	289, 345, 1556
BplI	GAGNNNNNCTC	2 cut(s)	136, 168
Bpu10I	CCTNAGC	2 cut(s)	1583, 2433
Bpu14I	TTCGAA	2 cut(s)	627, 1210
BpuEI	CTTGAG	1 cut(s)	2551
Bsa29I	ATCGAT	1 cut(s)	2574
BsaAI	YACGTR	1 cut(s)	1940
BsaJI	CCNNGG	6 cut(s)	1237, 1319, 1709, 1710, 2184, 2193
BsaWI	WCCGGW	2 cut(s)	204, 1886
BsaXI	ACNNNNNCTCC	4 cut(s)	1731, 1761, 2243, 2273
Bsc4I	CCNNNNNNNGG	2 cut(s)	1238, 2391
Bse1I	ACTGG	3 cut(s)	441, 524, 2325
Bse3DI	GCAATG	7 cut(s)	573, 873, 887, 1575, 2028, 2132, 2274
BseBI	CCWGG	2 cut(s)	1711, 1948
BseCI	ATCGAT	1 cut(s)	2574
BseDI	CCNNGG	6 cut(s)	1237, 1319, 1709, 1710, 2184, 2193
BseGI	GGATG	8 cut(s)	157, 373, 520, 930, 1084, 1232, 1329, 2603
BseLI	CCNNNNNNNGG	2 cut(s)	1238, 2391
BseMI	GCAATG	7 cut(s)	573, 873, 887, 1575, 2028, 2132, 2274
BseMII	CTCAG	4 cut(s)	1472, 1597, 2424, 2478
BseNI	ACTGG	3 cut(s)	441, 524, 2325
BseRI	GAGGAG	4 cut(s)	653, 656, 800, 894
BseSI	GKGCMC	2 cut(s)	1259, 2382
BseXI	GCAGC	6 cut(s)	1811, 1994, 2230, 2443, 2465, 2625
BseYI	CCCAGC	1 cut(s)	697
BsgI	GTGCAG	1 cut(s)	1445
Bsh1236I	CGCG	1 cut(s)	1160
BshFI	GGCC	3 cut(s)	1014, 1157, 2345
BshVI	ATCGAT	1 cut(s)	2574
BsiHKAI	GWGCWC	4 cut(s)	149, 1259, 1646, 2067
BsiHKCI	CYCGRG	1 cut(s)	554
BsiSI	CCGG	2 cut(s)	205, 1887
BslFI	GGGAC	1 cut(s)	80
BslI	CCNNNNNNNGG	2 cut(s)	1238, 2391
BsmAI	GTCTC	3 cut(s)	878, 1376, 1526
BsmFI	GGGAC	1 cut(s)	80
BsmI	GAATGC	2 cut(s)	41, 2657
BsnI	GGCC	3 cut(s)	1014, 1157, 2345
BsoBI	CYCGRG	1 cut(s)	554
Bsp119I	TTCGAA	2 cut(s)	627, 1210
Bsp1286I	GDGCHC	5 cut(s)	149, 1259, 1646, 2067, 2382
Bsp1407I	TGTACA	2 cut(s)	733, 966
Bsp143I	GATC	8 cut(s)	397, 993, 1188, 1269, 1447, 1675, 2155, 2413
BspACI	CCGC	6 cut(s)	9, 857, 1158, 1414, 2246, 2513
BspANI	GGCC	3 cut(s)	1014, 1157, 2345
BspCNI	CTCAG	4 cut(s)	1471, 1596, 2425, 2479
BspDI	ATCGAT	1 cut(s)	2574
BspFNI	CGCG	1 cut(s)	1160
BspLI	GGNNCC	4 cut(s)	203, 1137, 1310, 2190
BspMAI	CTGCAG	1 cut(s)	2461
BspMI	ACCTGC	1 cut(s)	883
BspPI	GGATC	3 cut(s)	405, 1455, 2150
BspQI	GCTCTTC	1 cut(s)	2055
BspT104I	TTCGAA	2 cut(s)	627, 1210
BspTI	CTTAAG	3 cut(s)	401, 458, 1969
BsrBI	CCGCTC	1 cut(s)	11
BsrDI	GCAATG	7 cut(s)	573, 873, 887, 1575, 2028, 2132, 2274
BsrGI	TGTACA	2 cut(s)	733, 966
BsrI	ACTGG	3 cut(s)	441, 524, 2325
BssECI	CCNNGG	6 cut(s)	1237, 1319, 1709, 1710, 2184, 2193
BssMI	GATC	8 cut(s)	397, 993, 1188, 1269, 1447, 1675, 2155, 2413
BssT1I	CCWWGG	3 cut(s)	1237, 2184, 2193
Bst2UI	CCWGG	2 cut(s)	1711, 1948
Bst4CI	ACNGT	9 cut(s)	89, 601, 658, 1145, 1384, 1504, 1555, 1747, 1917
Bst6I	CTCTTC	6 cut(s)	717, 997, 1061, 1434, 2040, 2055
BstAFI	CTTAAG	3 cut(s)	401, 458, 1969
BstAPI	GCANNNNNTGC	1 cut(s)	1577
BstAUI	TGTACA	2 cut(s)	733, 966
BstBAI	YACGTR	1 cut(s)	1940
BstBI	TTCGAA	2 cut(s)	627, 1210
BstDEI	CTNAG	7 cut(s)	684, 990, 1090, 1458, 1583, 2433, 2487
BstF5I	GGATG	8 cut(s)	157, 373, 520, 930, 1084, 1232, 1329, 2603
BstFNI	CGCG	1 cut(s)	1160
BstH2I	RGCGCY	2 cut(s)	654, 2184
BstHHI	GCGC	5 cut(s)	498, 653, 1162, 1582, 2183
BstKTI	GATC	8 cut(s)	400, 996, 1191, 1272, 1450, 1678, 2158, 2416
BstMAI	GTCTC	3 cut(s)	878, 1376, 1526
BstMBI	GATC	8 cut(s)	397, 993, 1188, 1269, 1447, 1675, 2155, 2413
BstMWI	GCNNNNNNNGC	9 cut(s)	113, 149, 473, 908, 1423, 1577, 1641, 2453, 2622
BstNI	CCWGG	2 cut(s)	1711, 1948
BstNSI	RCATGY	7 cut(s)	835, 853, 1411, 1838, 1931, 2231, 2565
BstSCI	CCNGG	2 cut(s)	1709, 1946
BstSFI	CTRYAG	4 cut(s)	88, 2151, 2442, 2457
BstSLI	GKGCMC	2 cut(s)	1259, 2382
BstUI	CGCG	1 cut(s)	1160
BstV1I	GCAGC	6 cut(s)	1811, 1994, 2230, 2443, 2465, 2625
BstV2I	GAAGAC	3 cut(s)	289, 345, 1556
BstX2I	RGATCY	5 cut(s)	397, 1269, 1447, 1675, 2155
BstXI	CCANNNNNNTGG	1 cut(s)	1286
BstYI	RGATCY	5 cut(s)	397, 1269, 1447, 1675, 2155
Bsu15I	ATCGAT	1 cut(s)	2574
BsuI	GTATCC	3 cut(s)	580, 601, 2078
BsuRI	GGCC	3 cut(s)	1014, 1157, 2345
BsuTUI	ATCGAT	1 cut(s)	2574
BtgZI	GCGATG	1 cut(s)	349
BtrI	CACGTC	1 cut(s)	2530
BtsCI	GGATG	8 cut(s)	157, 373, 520, 930, 1084, 1232, 1329, 2603
BtsI	GCAGTG	1 cut(s)	505
BtsIMutI	CAGTG	3 cut(s)	505, 1773, 1922
BveI	ACCTGC	1 cut(s)	883
CfoI	GCGC	5 cut(s)	498, 653, 1162, 1582, 2183
Cfr13I	GGNCC	3 cut(s)	790, 1247, 1308
ClaI	ATCGAT	1 cut(s)	2574
CseI	GACGC	1 cut(s)	482
Csp6I	GTAC	7 cut(s)	734, 837, 928, 967, 1838, 2073, 2618
CspCI	CAANNNNNGTGG	2 cut(s)	152, 187
CviQI	GTAC	7 cut(s)	734, 837, 928, 967, 1838, 2073, 2618
DdeI	CTNAG	7 cut(s)	684, 990, 1090, 1458, 1583, 2433, 2487
DpnI	GATC	8 cut(s)	399, 995, 1190, 1271, 1449, 1677, 2157, 2415
DpnII	GATC	8 cut(s)	397, 993, 1188, 1269, 1447, 1675, 2155, 2413
DraI	TTTAAA	1 cut(s)	2238
DraIII	CACNNNGTG	1 cut(s)	1943
Eam1104I	CTCTTC	6 cut(s)	717, 997, 1061, 1434, 2040, 2055
EarI	CTCTTC	6 cut(s)	717, 997, 1061, 1434, 2040, 2055
Ecl136II	GAGCTC	2 cut(s)	1644, 2065
Eco130I	CCWWGG	3 cut(s)	1237, 2184, 2193
Eco147I	AGGCCT	1 cut(s)	1014
Eco24I	GRGCYC	2 cut(s)	1646, 2067
Eco32I	GATATC	1 cut(s)	221
Eco47I	GGWCC	3 cut(s)	790, 1247, 1308
Eco53kI	GAGCTC	2 cut(s)	1644, 2065
Eco57I	CTGAAG	6 cut(s)	246, 276, 339, 999, 1134, 2061
Eco88I	CYCGRG	1 cut(s)	554
EcoICRI	GAGCTC	2 cut(s)	1644, 2065
EcoRI	GAATTC	1 cut(s)	247
EcoRII	CCWGG	2 cut(s)	1709, 1946
EcoRV	GATATC	1 cut(s)	221
EcoT14I	CCWWGG	3 cut(s)	1237, 2184, 2193
EcoT22I	ATGCAT	2 cut(s)	1762, 1836
EcoT38I	GRGCYC	2 cut(s)	1646, 2067
ErhI	CCWWGG	3 cut(s)	1237, 2184, 2193
FalI	AAGNNNNNCTT	2 cut(s)	2303, 2335
FaqI	GGGAC	1 cut(s)	80
FauI	CCCGC	1 cut(s)	2239
FblI	GTMKAC	1 cut(s)	2603
Fnu4HI	GCNGC	8 cut(s)	1158, 1415, 1800, 1983, 2244, 2454, 2457, 2614
FokI	GGATG	8 cut(s)	144, 380, 507, 937, 1091, 1219, 1336, 2610
FriOI	GRGCYC	2 cut(s)	1646, 2067
Fsp4HI	GCNGC	8 cut(s)	1158, 1415, 1800, 1983, 2244, 2454, 2457, 2614
FspBI	CTAG	9 cut(s)	231, 278, 773, 908, 1418, 1647, 1671, 2585, 2622
GlaI	GCGC	5 cut(s)	497, 652, 1161, 1581, 2182
GluI	GCNGC	8 cut(s)	1158, 1415, 1800, 1983, 2244, 2454, 2457, 2614
GsaI	CCCAGC	1 cut(s)	701
HaeII	RGCGCY	2 cut(s)	654, 2184
HaeIII	GGCC	3 cut(s)	1014, 1157, 2345
HapII	CCGG	2 cut(s)	205, 1887
HgaI	GACGC	1 cut(s)	482
HhaI	GCGC	5 cut(s)	498, 653, 1162, 1582, 2183
Hin6I	GCGC	5 cut(s)	496, 651, 1160, 1580, 2181
HinP1I	GCGC	5 cut(s)	496, 651, 1160, 1580, 2181
HindIII	AAGCTT	1 cut(s)	258
HinfI	GANTC	8 cut(s)	123, 728, 822, 1072, 1302, 1369, 2036, 2252
HpaII	CCGG	2 cut(s)	205, 1887
HphI	GGTGA	3 cut(s)	417, 1117, 2209
Hpy166II	GTNNAC	4 cut(s)	969, 1257, 1704, 2604
Hpy188III	TCNNGA	9 cut(s)	127, 582, 997, 1076, 1306, 1542, 1585, 1647, 2648
Hpy8I	GTNNAC	4 cut(s)	969, 1257, 1704, 2604
Hpy99I	CGWCG	1 cut(s)	2534
HpyAV	CCTTC	8 cut(s)	24, 192, 668, 767, 970, 2086, 2369, 2528
HpyCH4III	ACNGT	9 cut(s)	89, 601, 658, 1145, 1384, 1504, 1555, 1747, 1917
HpyCH4IV	ACGT	2 cut(s)	1939, 2529
HpyF10VI	GCNNNNNNNGC	9 cut(s)	113, 149, 473, 908, 1423, 1577, 1641, 2453, 2622
HpyF3I	CTNAG	7 cut(s)	684, 990, 1090, 1458, 1583, 2433, 2487
HpySE526I	ACGT	2 cut(s)	1939, 2529
HspAI	GCGC	5 cut(s)	496, 651, 1160, 1580, 2181
Kzo9I	GATC	8 cut(s)	397, 993, 1188, 1269, 1447, 1675, 2155, 2413
LguI	GCTCTTC	1 cut(s)	2055
LmnI	GCTCC	8 cut(s)	16, 121, 907, 1241, 1739, 1975, 2070, 2194
Lsp1109I	GCAGC	6 cut(s)	1811, 1994, 2230, 2443, 2465, 2625
LweI	GCATC	5 cut(s)	172, 337, 756, 2366, 2580
MaeI	CTAG	9 cut(s)	231, 278, 773, 908, 1418, 1647, 1671, 2585, 2622
MaeII	ACGT	2 cut(s)	1939, 2529
MaeIII	GTNAC	3 cut(s)	619, 1498, 2437
MalI	GATC	8 cut(s)	399, 995, 1190, 1271, 1449, 1677, 2157, 2415
MbiI	CCGCTC	1 cut(s)	11
MboI	GATC	8 cut(s)	397, 993, 1188, 1269, 1447, 1675, 2155, 2413
MflI	RGATCY	5 cut(s)	397, 1269, 1447, 1675, 2155
MhlI	GDGCHC	5 cut(s)	149, 1259, 1646, 2067, 2382
MlyI	GAGTC	4 cut(s)	132, 816, 1378, 2261
MmeI	TCCRAC	3 cut(s)	224, 540, 921
Mph1103I	ATGCAT	2 cut(s)	1762, 1836
MroXI	GAANNNNTTC	1 cut(s)	1471
MseI	TTAA	8 cut(s)	291, 402, 459, 1182, 1508, 1563, 1970, 2237
MslI	CAYNNNNRTG	2 cut(s)	1482, 2303
MspA1I	CMGCKG	2 cut(s)	359, 2456
MspCI	CTTAAG	3 cut(s)	401, 458, 1969
MspI	CCGG	2 cut(s)	205, 1887
MspR9I	CCNGG	2 cut(s)	1711, 1948
Mva1269I	GAATGC	2 cut(s)	41, 2657
MvaI	CCWGG	2 cut(s)	1711, 1948
MvnI	CGCG	1 cut(s)	1160
MwoI	GCNNNNNNNGC	9 cut(s)	113, 149, 473, 908, 1423, 1577, 1641, 2453, 2622
NdeII	GATC	8 cut(s)	397, 993, 1188, 1269, 1447, 1675, 2155, 2413
NlaIV	GGNNCC	4 cut(s)	203, 1137, 1310, 2190
NsiI	ATGCAT	2 cut(s)	1762, 1836
NspI	RCATGY	7 cut(s)	835, 853, 1411, 1838, 1931, 2231, 2565
NspV	TTCGAA	2 cut(s)	627, 1210
PaeI	GCATGC	1 cut(s)	2565
PaeR7I	CTCGAG	1 cut(s)	554
PasI	CCCWGGG	1 cut(s)	1710
PceI	AGGCCT	1 cut(s)	1014
PciSI	GCTCTTC	1 cut(s)	2055
PctI	GAATGC	2 cut(s)	41, 2657
PdmI	GAANNNNTTC	1 cut(s)	1471
PfeI	GAWTC	4 cut(s)	728, 1072, 1302, 2036
PflMI	CCANNNNNTGG	2 cut(s)	1238, 2391
PkrI	GCNGC	8 cut(s)	1159, 1416, 1801, 1984, 2245, 2455, 2458, 2615
PleI	GAGTC	4 cut(s)	131, 816, 1377, 2260
PpsI	GAGTC	4 cut(s)	131, 816, 1377, 2260
Ppu21I	YACGTR	1 cut(s)	1940
PsiI	TTATAA	2 cut(s)	1217, 1875
Psp124BI	GAGCTC	2 cut(s)	1646, 2067
Psp6I	CCWGG	2 cut(s)	1709, 1946
PspFI	CCCAGC	1 cut(s)	697
PspGI	CCWGG	2 cut(s)	1709, 1946
PspN4I	GGNNCC	4 cut(s)	203, 1137, 1310, 2190
PspPI	GGNCC	3 cut(s)	790, 1247, 1308
PspXI	VCTCGAGB	1 cut(s)	554
PstI	CTGCAG	1 cut(s)	2461
PsuI	RGATCY	5 cut(s)	397, 1269, 1447, 1675, 2155
PvuII	CAGCTG	2 cut(s)	359, 2456
RsaI	GTAC	7 cut(s)	735, 838, 929, 968, 1839, 2074, 2619
RsaNI	GTAC	7 cut(s)	734, 837, 928, 967, 1838, 2073, 2618
RseI	CAYNNNNRTG	2 cut(s)	1482, 2303
SacI	GAGCTC	2 cut(s)	1646, 2067
SapI	GCTCTTC	1 cut(s)	2055
SaqAI	TTAA	8 cut(s)	291, 402, 459, 1182, 1508, 1563, 1970, 2237
SatI	GCNGC	8 cut(s)	1158, 1415, 1800, 1983, 2244, 2454, 2457, 2614
Sau3AI	GATC	8 cut(s)	397, 993, 1188, 1269, 1447, 1675, 2155, 2413
Sau96I	GGNCC	3 cut(s)	790, 1247, 1308
SchI	GAGTC	4 cut(s)	132, 816, 1378, 2261
ScrFI	CCNGG	2 cut(s)	1711, 1948
SduI	GDGCHC	5 cut(s)	149, 1259, 1646, 2067, 2382
SfaNI	GCATC	5 cut(s)	172, 337, 756, 2366, 2580
SfcI	CTRYAG	4 cut(s)	88, 2151, 2442, 2457
Sfr274I	CTCGAG	1 cut(s)	554
SfuI	TTCGAA	2 cut(s)	627, 1210
SinI	GGWCC	3 cut(s)	790, 1247, 1308
SlaI	CTCGAG	1 cut(s)	554
SmiMI	CAYNNNNRTG	2 cut(s)	1482, 2303
SmlI	CTYRAG	5 cut(s)	401, 458, 554, 1969, 2566
SmoI	CTYRAG	5 cut(s)	401, 458, 554, 1969, 2566
SphI	GCATGC	1 cut(s)	2565
SseBI	AGGCCT	1 cut(s)	1014
SsiI	CCGC	6 cut(s)	9, 857, 1158, 1414, 2246, 2513
SspMI	CTAG	9 cut(s)	231, 278, 773, 908, 1418, 1647, 1671, 2585, 2622
SstI	GAGCTC	2 cut(s)	1646, 2067
StuI	AGGCCT	1 cut(s)	1014
StyD4I	CCNGG	2 cut(s)	1709, 1946
StyI	CCWWGG	3 cut(s)	1237, 2184, 2193
TaaI	ACNGT	9 cut(s)	89, 601, 658, 1145, 1384, 1504, 1555, 1747, 1917
TaiI	ACGT	2 cut(s)	1942, 2532
TaqI	TCGA	8 cut(s)	59, 555, 603, 627, 1210, 2416, 2532, 2574
TatI	WGTACW	3 cut(s)	733, 966, 1837
TauI	GCSGC	2 cut(s)	1160, 1417
TfiI	GAWTC	4 cut(s)	728, 1072, 1302, 2036
Tru1I	TTAA	8 cut(s)	291, 402, 459, 1182, 1508, 1563, 1970, 2237
Tru9I	TTAA	8 cut(s)	291, 402, 459, 1182, 1508, 1563, 1970, 2237
TscAI	CASTG	3 cut(s)	505, 1780, 1922
TseI	GCWGC	6 cut(s)	1799, 1982, 2243, 2453, 2456, 2613
TspGWI	ACGGA	2 cut(s)	84, 2148
TspRI	CASTG	3 cut(s)	505, 1780, 1922
Van91I	CCANNNNNTGG	2 cut(s)	1238, 2391
Vha464I	CTTAAG	3 cut(s)	401, 458, 1969
VneI	GTGCAC	1 cut(s)	1255
VpaK11BI	GGWCC	3 cut(s)	790, 1247, 1308
XapI	RAATTY	6 cut(s)	247, 540, 1056, 1625, 1652, 1964
XbaI	TCTAGA	1 cut(s)	1646
XceI	RCATGY	7 cut(s)	835, 853, 1411, 1838, 1931, 2231, 2565
XhoI	CTCGAG	1 cut(s)	554
XmiI	GTMKAC	1 cut(s)	2603
XmnI	GAANNNNTTC	1 cut(s)	1471
XspI	CTAG	9 cut(s)	231, 278, 773, 908, 1418, 1647, 1671, 2585, 2622
Zsp2I	ATGCAT	2 cut(s)	1762, 1836

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.