Rorug04G0066900

Region found in RelA / SpoT proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003029
Physical Location & Seq
Reverse (-)
10339827 .. 10342926
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0066900.1

Sequence Viewer

Length: 900 bp
ATGGAAGGCACTCTGGGATTACTTGGAGGTGGGTCTTCTGGGTGCTCAACAAATGAGTCAGCAATGTCAAAGGTGGAACAACAGGTGGTGGAGCAAGACTATGTGGGCATGTCCTCTGAGGTATCTTCATACCCAGCTGAAACTGAGCTTGAACTGGGCCTGAGCCTGGGTGGTGGTGGTGGTTCTGCACAGAAGCCCAAAGCCTGTGCATGGGGCGAGCGTGGAAGAATCTTGACTGCTAAAGACTTCCCTTCCATGGTGTCTCATGGGTCTGCAAGATTCTCACAGAGAGCAAATAATGCTTCTTCTGTTTCTGGAAATAAGAGGGCTGCTGAAGAGGCTGGATCTCCCACTGCTGTAAGTCAAGTTGTGGGATGGCCACCTATAAGTGCTGCAAGGATGAACAGCTTGGTTAACCAGGCAAAGACTGCAAGGTCTGAAGATGACAAGGCAGATGGGGACAAATCTAAGGACACTTACAAGAAGAAAATCAACACTGGTAATAAGTCAACTGTGAAAGAGAAAGGGCATCTGGGATTTGTGAAGGTCAACATGGATGGAATTCCTATAGGGAGGAAAGTGGATTTAAATGCTCACACTTGCTATGAGACTTTAGCTCAAACACTTGAGGACATGTTCTTCAGCACCACTACAACTGGCAATTCAATCGGTGGAGACAAGGAACAAGCAACAAAACCCTCAAGGCTTCTGGATGGATCCTCCGAGTTTGTGCTCACTTATGAAGATAAAGACGGAGACTGGATGCTTGTTGGAGATGTCCCTTGGAGGATGTTCCTCACCTCGGTGAAAAGGCTTCGAATAATGAGGACCTCCGAGGCCAACGGACTTGCTCCAAGATTTCAAGAAAGGAGTGAGAGACAAAGAAACAGACCTATATAA

Protein Analysis

299

Amino Acids

32.21

Weight (kDa)

8.32

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 47 - 286 1.7e-63 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 433
AclWI GGATC 3 cut(s) 352, 711, 724
AcoI YGGCCR 1 cut(s) 377
AcsI RAATTY 1 cut(s) 561
AcuI CTGAAG 3 cut(s) 354, 459, 625
AfiI CCNNNNNNNGG 4 cut(s) 166, 210, 256, 802
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 3 cut(s) 152, 666, 863
AjnI CCWGG 2 cut(s) 165, 417
AleI CACNNNNGTG 1 cut(s) 803
AluBI AGCT 4 cut(s) 137, 148, 408, 617
AluI AGCT 4 cut(s) 137, 148, 408, 617
Alw21I GWGCWC 2 cut(s) 47, 735
Alw26I GTCTC 5 cut(s) 267, 602, 669, 750, 871
AlwI GGATC 3 cut(s) 352, 711, 724
AoxI GGCC 3 cut(s) 157, 377, 837
ApeKI GCWGC 2 cut(s) 329, 392
ApoI RAATTY 1 cut(s) 561
AspS9I GGNCC 2 cut(s) 157, 828
AsuHPI GGTGA 2 cut(s) 790, 817
AsuII TTCGAA 1 cut(s) 817
AvaII GGWCC 1 cut(s) 828
BalI TGGCCA 1 cut(s) 379
BamHI GGATCC 1 cut(s) 716
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 2 cut(s) 47, 735
BbvI GCAGC 2 cut(s) 316, 379
BccI CCATC 4 cut(s) 369, 449, 551, 707
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 2 cut(s) 167, 419
BcoDI GTCTC 5 cut(s) 267, 602, 669, 750, 871
BfmI CTRYAG 1 cut(s) 567
BisI GCNGC 2 cut(s) 330, 393
BlsI GCNGC 2 cut(s) 331, 394
Bme1390I CCNGG 2 cut(s) 167, 419
Bme18I GGWCC 1 cut(s) 828
BmgT120I GGNCC 2 cut(s) 157, 828
BmiI GGNNCC 1 cut(s) 718
BmrFI CCNGG 2 cut(s) 167, 419
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 2 cut(s) 538, 753
BmuI ACTGGG 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 817
BpuEI CTTGAG 2 cut(s) 647, 685
BsaJI CCNNGG 5 cut(s) 166, 255, 782, 801, 834
Bsc4I CCNNNNNNNGG 4 cut(s) 166, 210, 256, 802
Bse1I ACTGG 4 cut(s) 159, 502, 661, 764
Bse3DI GCAATG 1 cut(s) 69
BseBI CCWGG 2 cut(s) 167, 419
BseDI CCNNGG 5 cut(s) 166, 255, 782, 801, 834
BseGI GGATG 6 cut(s) 380, 405, 562, 718, 768, 795
BseLI CCNNNNNNNGG 4 cut(s) 166, 210, 256, 802
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 3 cut(s) 108, 135, 152
BseNI ACTGG 4 cut(s) 159, 502, 661, 764
BseXI GCAGC 2 cut(s) 316, 379
BseYI CCCAGC 1 cut(s) 133
BsgI GTGCAG 1 cut(s) 171
BshFI GGCC 3 cut(s) 159, 379, 839
BsiHKAI GWGCWC 2 cut(s) 47, 735
BslFI GGGAC 2 cut(s) 473, 764
BslI CCNNNNNNNGG 4 cut(s) 166, 210, 256, 802
BsmAI GTCTC 5 cut(s) 267, 602, 669, 750, 871
BsmFI GGGAC 2 cut(s) 473, 764
BsnI GGCC 3 cut(s) 159, 379, 839
Bsp119I TTCGAA 1 cut(s) 817
Bsp1286I GDGCHC 2 cut(s) 47, 735
Bsp143I GATC 2 cut(s) 344, 716
Bsp19I CCATGG 1 cut(s) 255
BspANI GGCC 3 cut(s) 159, 379, 839
BspCNI CTCAG 3 cut(s) 109, 136, 153
BspLI GGNNCC 1 cut(s) 718
BspPI GGATC 3 cut(s) 352, 711, 724
BspT104I TTCGAA 1 cut(s) 817
BsrDI GCAATG 1 cut(s) 69
BsrI ACTGG 4 cut(s) 159, 502, 661, 764
BssECI CCNNGG 5 cut(s) 166, 255, 782, 801, 834
BssMI GATC 2 cut(s) 344, 716
BssT1I CCWWGG 2 cut(s) 255, 782
Bst2UI CCWGG 2 cut(s) 167, 419
Bst4CI ACNGT 1 cut(s) 514
Bst6I CTCTTC 1 cut(s) 330
BstAPI GCANNNNNTGC 2 cut(s) 299, 428
BstBI TTCGAA 1 cut(s) 817
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 4 cut(s) 117, 144, 161, 468
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 6 cut(s) 380, 405, 562, 718, 768, 795
BstKTI GATC 2 cut(s) 347, 719
BstMAI GTCTC 5 cut(s) 267, 602, 669, 750, 871
BstMBI GATC 2 cut(s) 344, 716
BstMWI GCNNNNNNNGC 3 cut(s) 299, 338, 428
BstNI CCWGG 2 cut(s) 167, 419
BstNSI RCATGY 2 cut(s) 112, 637
BstSCI CCNGG 2 cut(s) 165, 417
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 316, 379
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 2 cut(s) 344, 716
BstYI RGATCY 2 cut(s) 344, 716
BsuRI GGCC 3 cut(s) 159, 379, 839
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 6 cut(s) 380, 405, 562, 718, 768, 795
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 2 cut(s) 351, 495
Cac8I GCNNGC 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 157, 828
CviAII CATG 6 cut(s) 109, 210, 256, 266, 553, 634
DdeI CTNAG 4 cut(s) 117, 144, 161, 468
DpnI GATC 2 cut(s) 346, 718
DpnII GATC 2 cut(s) 344, 716
DraI TTTAAA 1 cut(s) 588
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 377
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 2 cut(s) 255, 782
Eco47I GGWCC 1 cut(s) 828
Eco57I CTGAAG 3 cut(s) 354, 459, 625
EcoO109I RGGNCCY 1 cut(s) 828
EcoRI GAATTC 1 cut(s) 561
EcoRII CCWGG 2 cut(s) 165, 417
EcoT14I CCWWGG 2 cut(s) 255, 782
ErhI CCWWGG 2 cut(s) 255, 782
FaeI CATG 6 cut(s) 112, 213, 259, 269, 556, 637
FaqI GGGAC 2 cut(s) 473, 764
FatI CATG 6 cut(s) 108, 209, 255, 265, 552, 633
Fnu4HI GCNGC 2 cut(s) 330, 393
FokI GGATG 6 cut(s) 387, 412, 569, 725, 775, 802
Fsp4HI GCNGC 2 cut(s) 330, 393
GluI GCNGC 2 cut(s) 330, 393
GsaI CCCAGC 1 cut(s) 137
HaeIII GGCC 3 cut(s) 159, 379, 839
Hin1II CATG 6 cut(s) 112, 213, 259, 269, 556, 637
HincII GTYRAC 3 cut(s) 415, 510, 550
HindII GTYRAC 3 cut(s) 415, 510, 550
HinfI GANTC 3 cut(s) 56, 228, 279
HpaI GTTAAC 1 cut(s) 415
HphI GGTGA 2 cut(s) 790, 817
Hpy166II GTNNAC 3 cut(s) 415, 510, 550
Hpy188I TCNGA 4 cut(s) 118, 439, 724, 835
Hpy188III TCNNGA 4 cut(s) 232, 315, 710, 863
Hpy8I GTNNAC 3 cut(s) 415, 510, 550
HpyAV CCTTC 2 cut(s) 261, 538
HpyCH4III ACNGT 1 cut(s) 514
HpyCH4V TGCA 5 cut(s) 188, 209, 275, 395, 431
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 338, 428
HpyF3I CTNAG 4 cut(s) 117, 144, 161, 468
Hsp92II CATG 6 cut(s) 112, 213, 259, 269, 556, 637
KspAI GTTAAC 1 cut(s) 415
Kzo9I GATC 2 cut(s) 344, 716
LmnI GCTCC 2 cut(s) 91, 856
Lsp1109I GCAGC 2 cut(s) 316, 379
LweI GCATC 2 cut(s) 538, 753
MalI GATC 2 cut(s) 346, 718
MboI GATC 2 cut(s) 344, 716
MboII GAAGA 9 cut(s) 27, 117, 237, 297, 347, 452, 496, 631, 755
MflI RGATCY 2 cut(s) 344, 716
MhlI GDGCHC 2 cut(s) 47, 735
MlsI TGGCCA 1 cut(s) 379
MluCI AATT 2 cut(s) 561, 661
MluNI TGGCCA 1 cut(s) 379
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 751
Mox20I TGGCCA 1 cut(s) 379
MscI TGGCCA 1 cut(s) 379
MseI TTAA 2 cut(s) 414, 587
MslI CAYNNNNRTG 1 cut(s) 803
Msp20I TGGCCA 1 cut(s) 379
MspA1I CMGCKG 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 167, 419
MvaI CCWGG 2 cut(s) 167, 419
MwoI GCNNNNNNNGC 3 cut(s) 299, 338, 428
NcoI CCATGG 1 cut(s) 255
NdeII GATC 2 cut(s) 344, 716
NlaIII CATG 6 cut(s) 112, 213, 259, 269, 556, 637
NlaIV GGNNCC 1 cut(s) 718
NspI RCATGY 2 cut(s) 112, 637
NspV TTCGAA 1 cut(s) 817
OliI CACNNNNGTG 1 cut(s) 803
PciI ACATGT 1 cut(s) 633
PfeI GAWTC 2 cut(s) 228, 279
PkrI GCNGC 2 cut(s) 331, 394
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
PpuMI RGGWCCY 1 cut(s) 828
PscI ACATGT 1 cut(s) 633
Psp5II RGGWCCY 1 cut(s) 828
Psp6I CCWGG 2 cut(s) 165, 417
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 2 cut(s) 165, 417
PspN4I GGNNCC 1 cut(s) 718
PspPI GGNCC 2 cut(s) 157, 828
PspPPI RGGWCCY 1 cut(s) 828
PsuI RGATCY 2 cut(s) 344, 716
PvuII CAGCTG 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 803
SaqAI TTAA 2 cut(s) 414, 587
SatI GCNGC 2 cut(s) 330, 393
Sau3AI GATC 2 cut(s) 344, 716
Sau96I GGNCC 2 cut(s) 157, 828
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 2 cut(s) 167, 419
SduI GDGCHC 2 cut(s) 47, 735
SfaNI GCATC 2 cut(s) 538, 753
SfcI CTRYAG 1 cut(s) 567
SfuI TTCGAA 1 cut(s) 817
SinI GGWCC 1 cut(s) 828
SmiI ATTTAAAT 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 803
SmlI CTYRAG 2 cut(s) 626, 700
SmoI CTYRAG 2 cut(s) 626, 700
Sse9I AATT 2 cut(s) 561, 661
StyD4I CCNGG 2 cut(s) 165, 417
StyI CCWWGG 2 cut(s) 255, 782
SwaI ATTTAAAT 1 cut(s) 588
TaaI ACNGT 1 cut(s) 514
TaqI TCGA 1 cut(s) 817
TasI AATT 2 cut(s) 561, 661
TfiI GAWTC 2 cut(s) 228, 279
Tru1I TTAA 2 cut(s) 414, 587
Tru9I TTAA 2 cut(s) 414, 587
TscAI CASTG 2 cut(s) 358, 502
TseI GCWGC 2 cut(s) 329, 392
TspDTI ATGAA 3 cut(s) 117, 416, 756
TspGWI ACGGA 2 cut(s) 768, 858
TspRI CASTG 2 cut(s) 358, 502
VpaK11BI GGWCC 1 cut(s) 828
XapI RAATTY 1 cut(s) 561
XceI RCATGY 2 cut(s) 112, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.