Rorug06G0244100

Region found in RelA / SpoT proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003029
Physical Location & Seq
Reverse (-)
38909729 .. 38911749
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0244100.1

Sequence Viewer

Length: 1557 bp
ATGGATAAGCAGGTGGCAATCGTCGGAGCTGGGATCAGTGGCCTTCTTGCCTGCAAGTACACCCTCTCGAAGGGCTTTCATCCCATTGTTTTCGAATCCACTAGCACCATTGGAGGCGTATGGACCAGGACTGTAGAGACTACAAAGCTCCAAACTCCTAAAGCATTCTACCAATTCTCAGATTTTCCATGGCCATCTTCTGTGAAAGAGGACCATCCTAACCAGAATCAGGTGATAGAATACATCCAGTCGTATGCTCGCCATTTCGACTTGCTCAAGCACATCAAATTCAATACCAAAGTGTGTGGTATAGAGTATGAAGGCCCATCTGAGGAGGAGATGCAATCTTGTAGTCTTTGGGGAGGCACTGGAGAAGCATTCAGCTCAGAAGGGAAATGGAAAGTTGTAGTAGAAGAGAAGCAAAGCCTTTCAACTAAGACTTATCTAGTAGACTTTGTGATCCTCTGCATCGGACGATTCAGTGATGTTCCAAACATTCCTGAATTCCCTCCGAACAACGGACCGGAAGCATTCCATGGAAAGGTTATACATTCGATGGAATACGCCGATATGGATTATGAAAGCGCTTCTAAATTCGTGACTGGAAAGCAAGTAACAGTTATCGGGTCTCAGAAATTTGCAATGGACATTGCAATGGAATGTTCAAATGCTAATGGGATAGAACATCCATGCACATTGTTGTACAGAACAGAACACTGGAATATACCAGATTTCTTTCCATGGGGTGTGCCTCTTCCATTACTCTACTTGAATCGCTTCTCCGAGCTCTTGGTTCATAAGCCTGGCGAAGGATTCCTGCTTAGTCTCCTTGCAGCACTGCTTTCACCTCTGAGATGGGCGTTTTCAAAATTTGTGGAAACATATGCAAATAACAAGCTAGGATTATACAAGTATGGGATGGTACCTAAGCAGACATTCCTTCAAGATATGAGTTCTGGTTTGGTAGCATTAGTGCCTGAAAAGTTTTATGACCGAGTGCAAGAGGGAAGCATCATACTCAAGAAATCTCCGAGAAACTTCGGATTTTGCGAAGAAGGAATTTTGGTTGAAGGGGAAGTCTCGCCTCTGAAGACGGATTTGGTCATATTGGCTACAGGCTTTACAGGTGAGAAGAAGCTCGAAGACATCTTTGTGTCTCCCACCTTTCAGAATTACATAACGGGACCGCCCGAGGCAATATTGCCCCTCTACAGGCATTGCATAAATGGACGAATTCCACAACTAGCTGTAATTGGTTTCTCCGAGAGTATCTCAAACTTGTACACCTCCGAGATGAGATGCAAATGGGTAACTGAGCTTCTTGGAGGCACGTTTAAGGTACCAAGCATAAAAGATATGGAAAAAGATGTGAAGAAATGGGACGAATATGCGAAGCGATATGGGGGAGAATACTACCGGAGATCATGCATCAGTGCCCTTCATTTATGGTACAACGACCAATTGTGCAAGGATATGGGATGGAACCCCAAGAGGAAGAAAGGACTGCTTGCTGAACTTTTTGAGCCTTATGGTCCAATGGATTATGTTTCCCCTTGA

Protein Analysis

518

Amino Acids

58.66

Weight (kDa)

6.06

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 3 - 497 1.7e-43 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 922, 1339
AccB1I GGYRCC 2 cut(s) 922, 1339
AccI GTMKAC 1 cut(s) 450
AciI CCGC 1 cut(s) 1187
AclWI GGATC 2 cut(s) 41, 454
AcoI YGGCCR 1 cut(s) 191
AcsI RAATTY 7 cut(s) 287, 503, 593, 635, 869, 1059, 1233
AcuI CTGAAG 1 cut(s) 1109
AfaI GTAC 6 cut(s) 59, 704, 924, 1283, 1341, 1451
AfeI AGCGCT 1 cut(s) 586
AfiI CCNNNNNNNGG 7 cut(s) 70, 229, 331, 518, 541, 809, 1212
AgsI TTSAA 7 cut(s) 292, 432, 666, 772, 867, 944, 1070
AjnI CCWGG 2 cut(s) 125, 802
AjuI GAANNNNNNNTTGG 2 cut(s) 1082, 1114
AluBI AGCT 8 cut(s) 29, 148, 384, 787, 898, 1138, 1247, 1318
AluI AGCT 8 cut(s) 29, 148, 384, 787, 898, 1138, 1247, 1318
Alw21I GWGCWC 1 cut(s) 789
Alw26I GTCTC 5 cut(s) 131, 633, 830, 1084, 1161
AlwI GGATC 2 cut(s) 41, 454
Ama87I CYCGRG 1 cut(s) 1190
Aor51HI AGCGCT 1 cut(s) 586
AoxI GGCC 3 cut(s) 40, 191, 322
ApeKI GCWGC 1 cut(s) 833
ApoI RAATTY 7 cut(s) 287, 503, 593, 635, 869, 1059, 1233
Asp700I GAANNNNTTC 1 cut(s) 776
Asp718I GGTACC 2 cut(s) 922, 1339
AspLEI GCGC 1 cut(s) 587
AspS9I GGNCC 6 cut(s) 123, 211, 323, 521, 1184, 1532
AsuHPI GGTGA 3 cut(s) 244, 837, 1139
AsuII TTCGAA 1 cut(s) 93
AvaI CYCGRG 1 cut(s) 1190
AvaII GGWCC 5 cut(s) 123, 211, 521, 1184, 1532
BaeGI GKGCMC 1 cut(s) 1438
BalI TGGCCA 1 cut(s) 193
BanI GGYRCC 2 cut(s) 922, 1339
BanII GRGCYC 1 cut(s) 789
BarI GAAGNNNNNNTAC 2 cut(s) 1302, 1334
BbsI GAAGAC 2 cut(s) 1097, 1149
Bbv12I GWGCWC 1 cut(s) 789
BbvI GCAGC 1 cut(s) 845
BccI CCATC 7 cut(s) 202, 222, 334, 550, 849, 913, 1473
BciT130I CCWGG 2 cut(s) 127, 804
BcoDI GTCTC 5 cut(s) 131, 633, 830, 1084, 1161
BfaI CTAG 4 cut(s) 102, 446, 899, 1244
BfmI CTRYAG 3 cut(s) 132, 1113, 1210
BfoI RGCGCY 1 cut(s) 588
BisI GCNGC 1 cut(s) 834
BlsI GCNGC 1 cut(s) 835
Bme1390I CCNGG 2 cut(s) 127, 804
Bme18I GGWCC 5 cut(s) 123, 211, 521, 1184, 1532
BmeT110I CYCGRG 1 cut(s) 1190
BmgT120I GGNCC 6 cut(s) 123, 211, 323, 521, 1184, 1532
BmiI GGNNCC 4 cut(s) 924, 1185, 1341, 1484
BmrFI CCNGG 2 cut(s) 127, 804
BmsI GCATC 5 cut(s) 330, 477, 1020, 1289, 1437
BpiI GAAGAC 2 cut(s) 1097, 1149
BplI GAGNNNNNCTC 2 cut(s) 1256, 1288
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 1 cut(s) 927
Bpu14I TTCGAA 1 cut(s) 93
BpuEI CTTGAG 2 cut(s) 260, 1004
BsaI GGTCTC 1 cut(s) 633
BsaJI CCNNGG 4 cut(s) 188, 535, 740, 1191
BsaWI WCCGGW 2 cut(s) 523, 1416
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 229, 331, 518, 541, 809, 1212
Bse1I ACTGG 4 cut(s) 247, 373, 607, 722
Bse3DI GCAATG 4 cut(s) 648, 648, 660, 1216
BseBI CCWGG 2 cut(s) 127, 804
BseDI CCNNGG 4 cut(s) 188, 535, 740, 1191
BseGI GGATG 6 cut(s) 79, 214, 243, 685, 924, 1484
BseLI CCNNNNNNNGG 7 cut(s) 70, 229, 331, 518, 541, 809, 1212
BseMI GCAATG 4 cut(s) 648, 648, 660, 1216
BseMII CTCAG 6 cut(s) 192, 321, 399, 644, 842, 1305
BseNI ACTGG 4 cut(s) 247, 373, 607, 722
BseRI GAGGAG 2 cut(s) 347, 350
BseSI GKGCMC 1 cut(s) 1438
BseXI GCAGC 1 cut(s) 845
BseYI CCCAGC 1 cut(s) 29
BshFI GGCC 3 cut(s) 42, 193, 324
BshNI GGYRCC 2 cut(s) 922, 1339
BsiHKAI GWGCWC 1 cut(s) 789
BsiHKCI CYCGRG 1 cut(s) 1190
BsiSI CCGG 2 cut(s) 524, 1417
BslFI GGGAC 2 cut(s) 1197, 1394
BslI CCNNNNNNNGG 7 cut(s) 70, 229, 331, 518, 541, 809, 1212
BsmAI GTCTC 5 cut(s) 131, 633, 830, 1084, 1161
BsmFI GGGAC 2 cut(s) 1197, 1394
BsmI GAATGC 3 cut(s) 164, 377, 530
BsnI GGCC 3 cut(s) 42, 193, 324
Bso31I GGTCTC 1 cut(s) 633
BsoBI CYCGRG 1 cut(s) 1190
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 789, 1438
Bsp1407I TGTACA 2 cut(s) 702, 1281
Bsp143I GATC 3 cut(s) 33, 459, 1421
Bsp19I CCATGG 3 cut(s) 188, 535, 740
BspACI CCGC 1 cut(s) 1187
BspANI GGCC 3 cut(s) 42, 193, 324
BspCNI CTCAG 6 cut(s) 191, 322, 398, 643, 843, 1306
BspLI GGNNCC 4 cut(s) 924, 1185, 1341, 1484
BspPI GGATC 2 cut(s) 41, 454
BspT104I TTCGAA 1 cut(s) 93
BspT107I GGYRCC 2 cut(s) 922, 1339
BspTNI GGTCTC 1 cut(s) 633
BsrDI GCAATG 4 cut(s) 648, 648, 660, 1216
BsrGI TGTACA 2 cut(s) 702, 1281
BsrI ACTGG 4 cut(s) 247, 373, 607, 722
BssECI CCNNGG 4 cut(s) 188, 535, 740, 1191
BssMI GATC 3 cut(s) 33, 459, 1421
BssT1I CCWWGG 3 cut(s) 188, 535, 740
Bst2UI CCWGG 2 cut(s) 127, 804
Bst4CI ACNGT 2 cut(s) 133, 619
Bst6I CTCTTC 2 cut(s) 408, 759
BstAUI TGTACA 2 cut(s) 702, 1281
BstBI TTCGAA 1 cut(s) 93
BstC8I GCNNGC 3 cut(s) 52, 259, 1509
BstDEI CTNAG 9 cut(s) 178, 330, 385, 435, 630, 821, 851, 927, 1314
BstDSI CCRYGG 3 cut(s) 188, 535, 740
BstENI CCTNNNNNAGG 2 cut(s) 68, 807
BstF5I GGATG 6 cut(s) 79, 214, 243, 685, 924, 1484
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 1 cut(s) 587
BstKTI GATC 3 cut(s) 36, 462, 1424
BstMAI GTCTC 5 cut(s) 131, 633, 830, 1084, 1161
BstMBI GATC 3 cut(s) 33, 459, 1421
BstNI CCWGG 2 cut(s) 127, 804
BstSCI CCNGG 2 cut(s) 125, 802
BstSFI CTRYAG 3 cut(s) 132, 1113, 1210
BstSLI GKGCMC 1 cut(s) 1438
BstV1I GCAGC 1 cut(s) 845
BstV2I GAAGAC 2 cut(s) 1097, 1149
BsuRI GGCC 3 cut(s) 42, 193, 324
BtgI CCRYGG 3 cut(s) 188, 535, 740
BtsCI GGATG 6 cut(s) 79, 214, 243, 685, 924, 1484
BtsI GCAGTG 1 cut(s) 836
BtsIMutI CAGTG 6 cut(s) 43, 366, 487, 715, 836, 1438
Cac8I GCNNGC 3 cut(s) 52, 259, 1509
CfoI GCGC 1 cut(s) 587
Cfr13I GGNCC 6 cut(s) 123, 211, 323, 521, 1184, 1532
CpoI CGGWCCG 1 cut(s) 521
Csp6I GTAC 6 cut(s) 58, 703, 923, 1282, 1340, 1450
CspCI CAANNNNNGTGG 4 cut(s) 286, 321, 855, 890
CspI CGGWCCG 1 cut(s) 521
CviAII CATG 5 cut(s) 189, 536, 690, 741, 1425
CviQI GTAC 6 cut(s) 58, 703, 923, 1282, 1340, 1450
DdeI CTNAG 9 cut(s) 178, 330, 385, 435, 630, 821, 851, 927, 1314
DpnI GATC 3 cut(s) 35, 461, 1423
DpnII GATC 3 cut(s) 33, 459, 1421
EaeI YGGCCR 1 cut(s) 191
Eam1104I CTCTTC 2 cut(s) 408, 759
EarI CTCTTC 2 cut(s) 408, 759
Ecl136II GAGCTC 1 cut(s) 787
Eco130I CCWWGG 3 cut(s) 188, 535, 740
Eco24I GRGCYC 1 cut(s) 789
Eco31I GGTCTC 1 cut(s) 633
Eco47I GGWCC 5 cut(s) 123, 211, 521, 1184, 1532
Eco47III AGCGCT 1 cut(s) 586
Eco53kI GAGCTC 1 cut(s) 787
Eco57I CTGAAG 1 cut(s) 1109
Eco88I CYCGRG 1 cut(s) 1190
EcoICRI GAGCTC 1 cut(s) 787
EcoNI CCTNNNNNAGG 2 cut(s) 68, 807
EcoRI GAATTC 2 cut(s) 503, 1233
EcoRII CCWGG 2 cut(s) 125, 802
EcoT14I CCWWGG 3 cut(s) 188, 535, 740
EcoT22I ATGCAT 1 cut(s) 1430
EcoT38I GRGCYC 1 cut(s) 789
ErhI CCWWGG 3 cut(s) 188, 535, 740
FaeI CATG 5 cut(s) 192, 539, 693, 744, 1428
FalI AAGNNNNNCTT 2 cut(s) 1491, 1523
FaqI GGGAC 2 cut(s) 1197, 1394
FatI CATG 5 cut(s) 188, 535, 689, 740, 1424
FauNDI CATATG 1 cut(s) 883
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 1 cut(s) 834
FokI GGATG 6 cut(s) 66, 201, 230, 672, 931, 1491
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 1 cut(s) 834
FspBI CTAG 4 cut(s) 102, 446, 899, 1244
GlaI GCGC 1 cut(s) 586
GluI GCNGC 1 cut(s) 834
GsaI CCCAGC 1 cut(s) 33
GsuI CTGGAG 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 588
HaeIII GGCC 3 cut(s) 42, 193, 324
HapII CCGG 2 cut(s) 524, 1417
HhaI GCGC 1 cut(s) 587
Hin1II CATG 5 cut(s) 192, 539, 693, 744, 1428
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HinfI GANTC 5 cut(s) 95, 226, 477, 772, 813
HpaII CCGG 2 cut(s) 524, 1417
HphI GGTGA 3 cut(s) 244, 837, 1139
Hpy166II GTNNAC 3 cut(s) 60, 451, 1284
Hpy188III TCNNGA 5 cut(s) 67, 500, 598, 944, 1021
Hpy8I GTNNAC 3 cut(s) 60, 451, 1284
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 9 cut(s) 53, 64, 314, 383, 803, 950, 1049, 1064, 1448
HpyCH4III ACNGT 2 cut(s) 133, 619
HpyCH4IV ACGT 1 cut(s) 1331
HpyF3I CTNAG 9 cut(s) 178, 330, 385, 435, 630, 821, 851, 927, 1314
HpySE526I ACGT 1 cut(s) 1331
Hsp92II CATG 5 cut(s) 192, 539, 693, 744, 1428
HspAI GCGC 1 cut(s) 585
KpnI GGTACC 2 cut(s) 926, 1343
Kzo9I GATC 3 cut(s) 33, 459, 1421
LmnI GCTCC 2 cut(s) 26, 153
Lsp1109I GCAGC 1 cut(s) 845
LweI GCATC 5 cut(s) 330, 477, 1020, 1289, 1437
MaeI CTAG 4 cut(s) 102, 446, 899, 1244
MaeII ACGT 1 cut(s) 1331
MaeIII GTNAC 3 cut(s) 598, 613, 1309
MalI GATC 3 cut(s) 35, 461, 1423
MboI GATC 3 cut(s) 33, 459, 1421
MboII GAAGA 9 cut(s) 189, 425, 746, 1064, 1102, 1144, 1154, 1384, 1507
MfeI CAATTG 1 cut(s) 1460
MhlI GDGCHC 2 cut(s) 789, 1438
MlsI TGGCCA 1 cut(s) 193
MluNI TGGCCA 1 cut(s) 193
MmeI TCCRAC 1 cut(s) 4
Mox20I TGGCCA 1 cut(s) 193
Mph1103I ATGCAT 1 cut(s) 1430
MroXI GAANNNNTTC 1 cut(s) 776
MscI TGGCCA 1 cut(s) 193
MseI TTAA 1 cut(s) 1335
MslI CAYNNNNRTG 2 cut(s) 653, 1151
Msp20I TGGCCA 1 cut(s) 193
MspI CCGG 2 cut(s) 524, 1417
MspR9I CCNGG 2 cut(s) 127, 804
MunI CAATTG 1 cut(s) 1460
Mva1269I GAATGC 3 cut(s) 164, 377, 530
MvaI CCWGG 2 cut(s) 127, 804
NcoI CCATGG 3 cut(s) 188, 535, 740
NdeI CATATG 1 cut(s) 883
NdeII GATC 3 cut(s) 33, 459, 1421
NlaIII CATG 5 cut(s) 192, 539, 693, 744, 1428
NlaIV GGNNCC 4 cut(s) 924, 1185, 1341, 1484
NmuCI GTSAC 1 cut(s) 598
NsiI ATGCAT 1 cut(s) 1430
NspV TTCGAA 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 1047
PctI GAATGC 3 cut(s) 164, 377, 530
PdmI GAANNNNTTC 1 cut(s) 776
PfeI GAWTC 5 cut(s) 95, 226, 477, 772, 813
PkrI GCNGC 1 cut(s) 835
Psp124BI GAGCTC 1 cut(s) 789
Psp6I CCWGG 2 cut(s) 125, 802
PspFI CCCAGC 1 cut(s) 29
PspGI CCWGG 2 cut(s) 125, 802
PspN4I GGNNCC 4 cut(s) 924, 1185, 1341, 1484
PspPI GGNCC 6 cut(s) 123, 211, 323, 521, 1184, 1532
RsaI GTAC 6 cut(s) 59, 704, 924, 1283, 1341, 1451
RsaNI GTAC 6 cut(s) 58, 703, 923, 1282, 1340, 1450
RseI CAYNNNNRTG 2 cut(s) 653, 1151
Rsr2I CGGWCCG 1 cut(s) 521
RsrII CGGWCCG 1 cut(s) 521
SacI GAGCTC 1 cut(s) 789
SaqAI TTAA 1 cut(s) 1335
SatI GCNGC 1 cut(s) 834
Sau3AI GATC 3 cut(s) 33, 459, 1421
Sau96I GGNCC 6 cut(s) 123, 211, 323, 521, 1184, 1532
ScrFI CCNGG 2 cut(s) 127, 804
SduI GDGCHC 2 cut(s) 789, 1438
SfaNI GCATC 5 cut(s) 330, 477, 1020, 1289, 1437
SfcI CTRYAG 3 cut(s) 132, 1113, 1210
SfuI TTCGAA 1 cut(s) 93
SinI GGWCC 5 cut(s) 123, 211, 521, 1184, 1532
SmiMI CAYNNNNRTG 2 cut(s) 653, 1151
SmlI CTYRAG 2 cut(s) 275, 1019
SmoI CTYRAG 2 cut(s) 275, 1019
SsiI CCGC 1 cut(s) 1187
SspI AATATT 1 cut(s) 1200
SspMI CTAG 4 cut(s) 102, 446, 899, 1244
SstI GAGCTC 1 cut(s) 789
StyD4I CCNGG 2 cut(s) 125, 802
StyI CCWWGG 3 cut(s) 188, 535, 740
TaaI ACNGT 2 cut(s) 133, 619
TaiI ACGT 1 cut(s) 1334
TaqI TCGA 5 cut(s) 68, 93, 267, 554, 1140
TaqII GACCGA 1 cut(s) 1008
TatI WGTACW 3 cut(s) 57, 702, 1281
TfiI GAWTC 5 cut(s) 95, 226, 477, 772, 813
Tru1I TTAA 1 cut(s) 1335
Tru9I TTAA 1 cut(s) 1335
TscAI CASTG 6 cut(s) 43, 373, 487, 722, 843, 1438
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 1 cut(s) 833
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 5 cut(s) 68, 333, 594, 785, 1430
TspGWI ACGGA 2 cut(s) 534, 1109
TspRI CASTG 6 cut(s) 43, 373, 487, 722, 843, 1438
VpaK11BI GGWCC 5 cut(s) 123, 211, 521, 1184, 1532
XagI CCTNNNNNAGG 2 cut(s) 68, 807
XapI RAATTY 7 cut(s) 287, 503, 593, 635, 869, 1059, 1233
XmiI GTMKAC 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 776
XspI CTAG 4 cut(s) 102, 446, 899, 1244
Zsp2I ATGCAT 1 cut(s) 1430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.