FvH4_4g13680

Expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005912
Physical Location & Seq
Forward (+)
17228623 .. 17229492
870 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13680.t1

Sequence Viewer

Length: 732 bp
ATGGGGAGTTTGGGACAACTCCGGCCAGCCTGGGACGAACTTGAAGCCCAAGTTAGCGTTAGCCTATTAGGACAAGGAAAAACCCAATTCGAAATCCGAAATGGAAGAGGATTTGCCTTTATAATCCCCTTCTCGCTTTACCTTTCTTCATCTATTCCCACTTCTCTTCCTATTCTCTCCCACTTTCCAAATCCATCTCCGTTTTTGTTGCCGAATTCTATGGAGCAGTGGAAGGAATTCTTCTCCACCGCGCCAAACAAATCCACTGTCTTCGACATACTCGATCACGCGATCGCCGTCGCCGCCTCAGATTTCCCAGATGAGTTCAGTCGCCAAAAGACTCGGCTCCTCAATTCGATTCAAGCTGCCGGCAGCGGCGGAAAATCTGAAACAGGGTCTGATCCAAGGCCATCACTCATAGTCCGCATCAAGATCCCGCCGAAATCGGCCGAGCCGGCCTCCTGTGTTGTTGTTGAAACTAGTAGTCCGATTAAGGCCAAGCCGTGCTTGGATGAGGAAGCAAAGATTCAAGCTACCAAGAGAAAGTTGCACGATCGGTACGAGGAAATTGAGAACACCAAGAGGCAAAAGAGGATTCAGGTGTTAAACTATGTTCCCAGCCAAGAATTTAAGGTTGCAATCCCTGCCAAGAAAGCAGCTAGAAAGCACTGTATGCCGGGGCGTAAGCCGCTTGGGTTTCAGAGTCTGCATAGCAGGCTAGTTAGGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

244

Amino Acids

26.97

Weight (kDa)

9.64

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 122
AccII CGCG 2 cut(s) 251, 290
AciI CCGC 7 cut(s) 249, 303, 375, 378, 424, 437, 689
AclWI GGATC 2 cut(s) 395, 427
AcoI YGGCCR 2 cut(s) 23, 447
AcsI RAATTY 3 cut(s) 214, 236, 626
AfaI GTAC 1 cut(s) 560
AgsI TTSAA 4 cut(s) 44, 362, 476, 530
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 1 cut(s) 29
AluBI AGCT 3 cut(s) 365, 533, 659
AluI AGCT 3 cut(s) 365, 533, 659
AlwI GGATC 2 cut(s) 395, 427
AlwNI CAGNNNCTG 2 cut(s) 398, 706
AoxI GGCC 5 cut(s) 23, 407, 447, 456, 495
ApeKI GCWGC 3 cut(s) 365, 372, 656
ApoI RAATTY 3 cut(s) 214, 236, 626
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
AsiSI GCGATCGC 1 cut(s) 294
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 1 cut(s) 253
AsuC2I CCSGG 1 cut(s) 678
AsuII TTCGAA 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 262
BbvI GCAGC 3 cut(s) 352, 384, 668
BccI CCATC 2 cut(s) 202, 418
BceAI ACGGC 2 cut(s) 281, 487
BciT130I CCWGG 1 cut(s) 31
BcnI CCSGG 1 cut(s) 678
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 3 cut(s) 480, 660, 719
BglI GCCNNNNNGGC 1 cut(s) 455
BisI GCNGC 6 cut(s) 303, 366, 373, 376, 657, 689
BlsI GCNGC 6 cut(s) 304, 367, 374, 377, 658, 690
Bme1390I CCNGG 2 cut(s) 31, 678
BmiI GGNNCC 1 cut(s) 347
BmrFI CCNGG 2 cut(s) 31, 678
BmsI GCATC 1 cut(s) 435
BpiI GAAGAC 1 cut(s) 262
BplI GAGNNNNNCTC 2 cut(s) 443, 475
Bpu14I TTCGAA 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 678
BsaJI CCNNGG 3 cut(s) 30, 404, 677
Bse118I RCCGGY 2 cut(s) 368, 454
BseBI CCWGG 1 cut(s) 31
BseDI CCNNGG 3 cut(s) 30, 404, 677
BseGI GGATG 1 cut(s) 517
BseMII CTCAG 1 cut(s) 321
BseRI GAGGAG 1 cut(s) 338
BseX3I CGGCCG 1 cut(s) 447
BseXI GCAGC 3 cut(s) 352, 384, 668
BseYI CCCAGC 1 cut(s) 617
Bsh1236I CGCG 2 cut(s) 251, 290
Bsh1285I CGRYCG 3 cut(s) 294, 450, 556
BshFI GGCC 5 cut(s) 25, 409, 449, 458, 497
BsiEI CGRYCG 3 cut(s) 294, 450, 556
BsiSI CCGG 4 cut(s) 22, 369, 455, 677
BslFI GGGAC 2 cut(s) 27, 47
BsmFI GGGAC 2 cut(s) 27, 47
BsnI GGCC 5 cut(s) 25, 409, 449, 458, 497
Bsp119I TTCGAA 1 cut(s) 90
Bsp143I GATC 5 cut(s) 283, 291, 400, 432, 553
BspACI CCGC 7 cut(s) 249, 303, 375, 378, 424, 437, 689
BspANI GGCC 5 cut(s) 25, 409, 449, 458, 497
BspCNI CTCAG 1 cut(s) 320
BspFNI CGCG 2 cut(s) 251, 290
BspLI GGNNCC 1 cut(s) 347
BspPI GGATC 2 cut(s) 395, 427
BspT104I TTCGAA 1 cut(s) 90
BsrFI RCCGGY 2 cut(s) 368, 454
BssAI RCCGGY 2 cut(s) 368, 454
BssECI CCNNGG 3 cut(s) 30, 404, 677
BssMI GATC 5 cut(s) 283, 291, 400, 432, 553
BssT1I CCWWGG 1 cut(s) 404
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 2 cut(s) 268, 671
Bst6I CTCTTC 2 cut(s) 100, 171
BstAPI GCANNNNNTGC 2 cut(s) 644, 673
BstBI TTCGAA 1 cut(s) 90
BstC8I GCNNGC 4 cut(s) 27, 370, 456, 716
BstDEI CTNAG 1 cut(s) 307
BstF5I GGATG 1 cut(s) 517
BstFNI CGCG 2 cut(s) 251, 290
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 5 cut(s) 286, 294, 403, 435, 556
BstMBI GATC 5 cut(s) 283, 291, 400, 432, 553
BstMCI CGRYCG 3 cut(s) 294, 450, 556
BstMWI GCNNNNNNNGC 7 cut(s) 302, 455, 644, 653, 673, 688, 715
BstNI CCWGG 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 29, 676
BstUI CGCG 2 cut(s) 251, 290
BstV1I GCAGC 3 cut(s) 352, 384, 668
BstV2I GAAGAC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 432
BstYI RGATCY 1 cut(s) 432
BstZI CGGCCG 1 cut(s) 447
BsuRI GGCC 5 cut(s) 25, 409, 449, 458, 497
BtsCI GGATG 1 cut(s) 517
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 3 cut(s) 233, 264, 667
Cac8I GCNNGC 4 cut(s) 27, 370, 456, 716
CaiI CAGNNNCTG 2 cut(s) 398, 706
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 2 cut(s) 368, 454
Csp6I GTAC 1 cut(s) 559
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 1 cut(s) 307
DpnI GATC 5 cut(s) 285, 293, 402, 434, 555
DpnII GATC 5 cut(s) 283, 291, 400, 432, 553
EaeI YGGCCR 2 cut(s) 23, 447
EagI CGGCCG 1 cut(s) 447
Eam1104I CTCTTC 2 cut(s) 100, 171
EarI CTCTTC 2 cut(s) 100, 171
EciI GGCGGA 1 cut(s) 393
EclXI CGGCCG 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 404
Eco52I CGGCCG 1 cut(s) 447
EcoRI GAATTC 2 cut(s) 214, 236
EcoRII CCWGG 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 404
ErhI CCWWGG 1 cut(s) 404
FaiI YATR 7 cut(s) 122, 221, 278, 419, 612, 674, 711
FalI AAGNNNNNCTT 4 cut(s) 224, 256, 491, 523
FaqI GGGAC 2 cut(s) 27, 47
FauI CCCGC 1 cut(s) 444
Fnu4HI GCNGC 6 cut(s) 303, 366, 373, 376, 657, 689
FokI GGATG 1 cut(s) 524
Fsp4HI GCNGC 6 cut(s) 303, 366, 373, 376, 657, 689
FspBI CTAG 3 cut(s) 480, 660, 719
GlaI GCGC 1 cut(s) 252
GluI GCNGC 6 cut(s) 303, 366, 373, 376, 657, 689
GsaI CCCAGC 1 cut(s) 621
HaeIII GGCC 5 cut(s) 25, 409, 449, 458, 497
HapII CCGG 4 cut(s) 22, 369, 455, 677
HhaI GCGC 1 cut(s) 253
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HinfI GANTC 5 cut(s) 340, 358, 526, 595, 703
HpaII CCGG 4 cut(s) 22, 369, 455, 677
Hpy188I TCNGA 6 cut(s) 98, 310, 388, 400, 489, 702
Hpy188III TCNNGA 1 cut(s) 430
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 2 cut(s) 139, 226
HpyCH4III ACNGT 2 cut(s) 268, 671
HpyCH4V TGCA 3 cut(s) 550, 638, 709
HpyF10VI GCNNNNNNNGC 7 cut(s) 302, 455, 644, 653, 673, 688, 715
HpyF3I CTNAG 1 cut(s) 307
HspAI GCGC 1 cut(s) 251
KroI GCCGGC 2 cut(s) 368, 454
KroNI GCCGGC 2 cut(s) 370, 456
Kzo9I GATC 5 cut(s) 283, 291, 400, 432, 553
LmnI GCTCC 2 cut(s) 223, 351
Lsp1109I GCAGC 3 cut(s) 352, 384, 668
LweI GCATC 1 cut(s) 435
MaeI CTAG 3 cut(s) 480, 660, 719
MalI GATC 5 cut(s) 285, 293, 402, 434, 555
MboI GATC 5 cut(s) 283, 291, 400, 432, 553
MboII GAAGA 5 cut(s) 117, 138, 158, 232, 262
MflI RGATCY 1 cut(s) 432
MluCI AATT 6 cut(s) 86, 214, 236, 352, 567, 626
MlyI GAGTC 2 cut(s) 334, 712
MnlI CCTC 8 cut(s) 101, 316, 359, 469, 508, 556, 576, 585
MroNI GCCGGC 2 cut(s) 368, 454
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 3 cut(s) 492, 605, 630
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 4 cut(s) 22, 369, 455, 677
MspR9I CCNGG 2 cut(s) 31, 678
MvaI CCWGG 1 cut(s) 31
MvnI CGCG 2 cut(s) 251, 290
MwoI GCNNNNNNNGC 7 cut(s) 302, 455, 644, 653, 673, 688, 715
NaeI GCCGGC 2 cut(s) 370, 456
NciI CCSGG 1 cut(s) 678
NdeII GATC 5 cut(s) 283, 291, 400, 432, 553
NgoMIV GCCGGC 2 cut(s) 368, 454
NlaIV GGNNCC 1 cut(s) 347
NmeAIII GCCGAG 2 cut(s) 322, 475
NspV TTCGAA 1 cut(s) 90
PcsI WCGNNNNNNNCGW 3 cut(s) 279, 294, 558
PdiI GCCGGC 2 cut(s) 370, 456
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 3 cut(s) 358, 526, 595
PkrI GCNGC 6 cut(s) 304, 367, 374, 377, 658, 690
Ple19I CGATCG 2 cut(s) 294, 556
PleI GAGTC 2 cut(s) 334, 711
PpsI GAGTC 2 cut(s) 334, 711
PsiI TTATAA 1 cut(s) 122
Psp6I CCWGG 1 cut(s) 29
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 347
PstNI CAGNNNCTG 2 cut(s) 398, 706
PsuI RGATCY 1 cut(s) 432
PvuI CGATCG 2 cut(s) 294, 556
RgaI GCGATCGC 1 cut(s) 294
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
SaqAI TTAA 3 cut(s) 492, 605, 630
SatI GCNGC 6 cut(s) 303, 366, 373, 376, 657, 689
Sau3AI GATC 5 cut(s) 283, 291, 400, 432, 553
SchI GAGTC 2 cut(s) 334, 712
ScrFI CCNGG 2 cut(s) 31, 678
SetI ASST 7 cut(s) 144, 367, 535, 603, 636, 661, 728
SfaAI GCGATCGC 1 cut(s) 294
SfaNI GCATC 1 cut(s) 435
SfiI GGCCNNNNNGGCC 1 cut(s) 455
SfuI TTCGAA 1 cut(s) 90
SgfI GCGATCGC 1 cut(s) 294
SpeI ACTAGT 1 cut(s) 479
Sse9I AATT 6 cut(s) 86, 214, 236, 352, 567, 626
SsiI CCGC 7 cut(s) 249, 303, 375, 378, 424, 437, 689
SspMI CTAG 3 cut(s) 480, 660, 719
StyD4I CCNGG 2 cut(s) 29, 676
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 2 cut(s) 268, 671
TaqI TCGA 4 cut(s) 90, 273, 282, 356
TasI AATT 6 cut(s) 86, 214, 236, 352, 567, 626
TauI GCSGC 3 cut(s) 305, 378, 691
TfiI GAWTC 3 cut(s) 358, 526, 595
Tru1I TTAA 3 cut(s) 492, 605, 630
Tru9I TTAA 3 cut(s) 492, 605, 630
TscAI CASTG 3 cut(s) 233, 271, 674
TseI GCWGC 3 cut(s) 365, 372, 656
TspDTI ATGAA 1 cut(s) 138
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 3 cut(s) 233, 271, 674
XapI RAATTY 3 cut(s) 214, 236, 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 505
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 3 cut(s) 480, 660, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.