Rmu_sc0024961.1_g000001

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0024961.1
OG0005912
Physical Location & Seq
Reverse (-)
806 .. 3460
2655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0024961.1_g000001.1.cds

Sequence Viewer

Length: 1026 bp
atgatagggagaatggcggatctgatccagagttggttgagtctggttctcctggaaaagtggcttatggtttctatcatattagaaatgttaatcagttgcgtatggatgacattccaaagctgctggcagagtacaaacaactacttaattaagggagggacgtgcctttggtctctcacccagtccaaagctcgcaagtgccctccaatctttgccaaaatggaccacgagatcaccgacttagaggcttttgccttgtttgcaagtcttttcgaaagagtctttattcttaagagaaagtttcccaattctcgatctctaggctctagctgtttgttcgttcattactttcgatctcagttttcttccatggcgcgggcgatcccgttttcttccatggcggcgccgatccagaagaaaccagagtcgctccagtactggagggatttctttgccactgcaaacaactccacctccaccattttcgatattctcgaccacgccatcaccgtcgccgcctctgatttcccggaggagttcactcgccagaggactcgaattctcgattcgattcaacgtttgggtttgggcggcggttctgatctagggttttgtgctggtgctgcatctggaagttctgggtccgatcaaaaggctgagcagccttcaaagccatcgctcaaaatacgcataaagatcgtgaagcgaaatgttgatggtaccctgatcgaaccagcgaaattgatcgagccctgttctcatgatctgatcaagcccgccgcaccctgtgttggcgttggtgttggtgatcaagcaaaattggatgaagcggctattgaggccaagcttcaggccacaaagagaaagctccacgatcgatatcaagaaatcgagaaccataagaggcagaggaggatacaagtcttaaaccatcttcccaggcaagacttcaggattggtcgatgccctgctaaacccagacttggaaaccattggaaaccagtactaaaaatcagaacataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

341

Amino Acids

38.48

Weight (kDa)

9.64

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 722
AccB1I GGYRCC 2 cut(s) 406, 722
AccII CGCG 1 cut(s) 379
AciI CCGC 9 cut(s) 17, 379, 404, 519, 594, 597, 780, 783, 833
AclI AACGTT 1 cut(s) 580
AclWI GGATC 4 cut(s) 19, 27, 379, 406
AcsI RAATTY 1 cut(s) 561
AcuI CTGAAG 2 cut(s) 836, 937
AcyI GRCGYC 1 cut(s) 407
AdeI CACNNNGTG 1 cut(s) 791
AfaI GTAC 4 cut(s) 136, 440, 724, 1008
AfiI CCNNNNNNNGG 3 cut(s) 378, 794, 986
AflII CTTAAG 1 cut(s) 293
AgsI TTSAA 2 cut(s) 578, 672
AjiI CACGTC 1 cut(s) 165
AjnI CCWGG 2 cut(s) 51, 941
AluBI AGCT 5 cut(s) 123, 194, 333, 850, 871
AluI AGCT 5 cut(s) 123, 194, 333, 850, 871
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 4 cut(s) 19, 27, 379, 406
AoxI GGCC 2 cut(s) 843, 855
ApeKI GCWGC 3 cut(s) 123, 626, 664
ApoI RAATTY 1 cut(s) 561
Asp718I GGTACC 1 cut(s) 722
AspLEI GCGC 2 cut(s) 379, 409
AspS9I GGNCC 2 cut(s) 226, 645
AsuC2I CCSGG 1 cut(s) 533
AsuHPI GGTGA 4 cut(s) 172, 229, 502, 821
AsuII TTCGAA 1 cut(s) 276
AvaII GGWCC 2 cut(s) 226, 645
BaeGI GKGCMC 1 cut(s) 206
BanI GGYRCC 2 cut(s) 406, 722
BanII GRGCYC 1 cut(s) 756
BauI CACGAG 1 cut(s) 230
BbvI GCAGC 3 cut(s) 110, 613, 676
BccI CCATC 4 cut(s) 515, 685, 713, 942
BcgI CGANNNNNNTGC 2 cut(s) 682, 716
BciT130I CCWGG 2 cut(s) 53, 943
BciVI GTATCC 1 cut(s) 912
BclI TGATCA 2 cut(s) 771, 811
BcnI CCSGG 1 cut(s) 533
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 3 cut(s) 323, 330, 608
BfoI RGCGCY 1 cut(s) 410
BfrI CTTAAG 1 cut(s) 293
BfuI GTATCC 1 cut(s) 912
BisI GCNGC 8 cut(s) 124, 405, 519, 595, 627, 665, 783, 834
BlpI GCTNAGC 1 cut(s) 660
BlsI GCNGC 8 cut(s) 125, 406, 520, 596, 628, 666, 784, 835
BmcAI AGTACT 2 cut(s) 440, 1008
Bme1390I CCNGG 3 cut(s) 53, 533, 943
Bme18I GGWCC 2 cut(s) 226, 645
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 2 cut(s) 226, 645
BmiI GGNNCC 3 cut(s) 408, 646, 724
BmrFI CCNGG 3 cut(s) 53, 533, 943
BmrI ACTGGG 1 cut(s) 178
BmsI GCATC 2 cut(s) 638, 956
BmuI ACTGGG 1 cut(s) 178
BpmI CTGGAG 2 cut(s) 419, 463
Bpu1102I GCTNAGC 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 533
Bsa29I ATCGAT 1 cut(s) 880
BsaBI GATNNNNATC 1 cut(s) 882
BsaHI GRCGYC 1 cut(s) 407
BsaI GGTCTC 1 cut(s) 180
BsaJI CCNNGG 3 cut(s) 372, 399, 941
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 3 cut(s) 378, 794, 986
Bse1I ACTGG 4 cut(s) 184, 436, 446, 1004
Bse8I GATNNNNATC 1 cut(s) 882
BseBI CCWGG 2 cut(s) 53, 943
BseCI ATCGAT 1 cut(s) 880
BseDI CCNNGG 3 cut(s) 372, 399, 941
BseGI GGATG 2 cut(s) 114, 832
BseJI GATNNNNATC 1 cut(s) 882
BseLI CCNNNNNNNGG 3 cut(s) 378, 794, 986
BseMII CTCAG 2 cut(s) 374, 651
BseNI ACTGG 4 cut(s) 184, 436, 446, 1004
BseRI GAGGAG 2 cut(s) 551, 928
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 3 cut(s) 110, 613, 676
Bsh1236I CGCG 1 cut(s) 379
Bsh1285I CGRYCG 1 cut(s) 880
BshFI GGCC 2 cut(s) 845, 857
BshNI GGYRCC 2 cut(s) 406, 722
BshVI ATCGAT 1 cut(s) 880
BsiEI CGRYCG 1 cut(s) 880
BsiSI CCGG 1 cut(s) 533
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 3 cut(s) 378, 794, 986
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 175
BsnI GGCC 2 cut(s) 845, 857
Bso31I GGTCTC 1 cut(s) 180
Bsp119I TTCGAA 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 206, 756
Bsp1720I GCTNAGC 1 cut(s) 660
Bsp19I CCATGG 2 cut(s) 372, 399
BspACI CCGC 9 cut(s) 17, 379, 404, 519, 594, 597, 780, 783, 833
BspANI GGCC 2 cut(s) 845, 857
BspCNI CTCAG 2 cut(s) 373, 652
BspDI ATCGAT 1 cut(s) 880
BspFNI CGCG 1 cut(s) 379
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 3 cut(s) 408, 646, 724
BspPI GGATC 4 cut(s) 19, 27, 379, 406
BspT104I TTCGAA 1 cut(s) 276
BspT107I GGYRCC 2 cut(s) 406, 722
BspTI CTTAAG 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 180
BsrI ACTGG 4 cut(s) 184, 436, 446, 1004
BssECI CCNNGG 3 cut(s) 372, 399, 941
BssNI GRCGYC 1 cut(s) 407
BssSI CACGAG 1 cut(s) 230
BssT1I CCWWGG 2 cut(s) 372, 399
Bst2BI CACGAG 1 cut(s) 230
Bst2UI CCWGG 2 cut(s) 53, 943
Bst4CI ACNGT 1 cut(s) 514
BstACI GRCGYC 1 cut(s) 407
BstAFI CTTAAG 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 276
BstC8I GCNNGC 4 cut(s) 128, 196, 381, 780
BstDEI CTNAG 3 cut(s) 244, 360, 660
BstDSI CCRYGG 2 cut(s) 372, 399
BstF5I GGATG 2 cut(s) 114, 832
BstFNI CGCG 1 cut(s) 379
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 2 cut(s) 379, 409
BstMAI GTCTC 1 cut(s) 180
BstMCI CGRYCG 1 cut(s) 880
BstMWI GCNNNNNNNGC 4 cut(s) 263, 626, 673, 842
BstNI CCWGG 2 cut(s) 53, 943
BstSCI CCNGG 3 cut(s) 51, 531, 941
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 1 cut(s) 379
BstV1I GCAGC 3 cut(s) 110, 613, 676
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 880
BsuI GTATCC 1 cut(s) 912
BsuRI GGCC 2 cut(s) 845, 857
BsuTUI ATCGAT 1 cut(s) 880
BtgI CCRYGG 2 cut(s) 372, 399
BtgZI GCGATG 1 cut(s) 663
BtrI CACGTC 1 cut(s) 165
BtsCI GGATG 2 cut(s) 114, 832
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 1 cut(s) 459
Cac8I GCNNGC 4 cut(s) 128, 196, 381, 780
CciI TCATGA 1 cut(s) 763
CfoI GCGC 2 cut(s) 379, 409
Cfr13I GGNCC 2 cut(s) 226, 645
ClaI ATCGAT 1 cut(s) 880
Csp6I GTAC 4 cut(s) 135, 439, 723, 1007
CviAII CATG 3 cut(s) 373, 400, 764
CviQI GTAC 4 cut(s) 135, 439, 723, 1007
DdeI CTNAG 3 cut(s) 244, 360, 660
DinI GGCGCC 1 cut(s) 408
DraIII CACNNNGTG 1 cut(s) 791
EciI GGCGGA 1 cut(s) 32
Eco130I CCWWGG 2 cut(s) 372, 399
Eco24I GRGCYC 1 cut(s) 756
Eco31I GGTCTC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 884
Eco47I GGWCC 2 cut(s) 226, 645
Eco57I CTGAAG 2 cut(s) 836, 937
EcoRI GAATTC 1 cut(s) 561
EcoRII CCWGG 2 cut(s) 51, 941
EcoRV GATATC 1 cut(s) 884
EcoT14I CCWWGG 2 cut(s) 372, 399
EcoT38I GRGCYC 1 cut(s) 756
EgeI GGCGCC 1 cut(s) 408
EheI GGCGCC 1 cut(s) 408
ErhI CCWWGG 2 cut(s) 372, 399
FaeI CATG 3 cut(s) 376, 403, 767
FaiI YATR 9 cut(s) 68, 80, 106, 374, 401, 695, 765, 903, 1024
FaqI GGGAC 1 cut(s) 175
FatI CATG 3 cut(s) 372, 399, 763
FauI CCCGC 2 cut(s) 372, 787
FbaI TGATCA 2 cut(s) 771, 811
Fnu4HI GCNGC 8 cut(s) 124, 405, 519, 595, 627, 665, 783, 834
FokI GGATG 2 cut(s) 121, 839
FriOI GRGCYC 1 cut(s) 756
Fsp4HI GCNGC 8 cut(s) 124, 405, 519, 595, 627, 665, 783, 834
FspBI CTAG 3 cut(s) 323, 330, 608
GlaI GCGC 2 cut(s) 378, 408
GluI GCNGC 8 cut(s) 124, 405, 519, 595, 627, 665, 783, 834
GsuI CTGGAG 2 cut(s) 419, 463
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 2 cut(s) 845, 857
HapII CCGG 1 cut(s) 533
HhaI GCGC 2 cut(s) 379, 409
Hin1I GRCGYC 1 cut(s) 407
Hin1II CATG 3 cut(s) 376, 403, 767
Hin6I GCGC 2 cut(s) 377, 407
HinP1I GCGC 2 cut(s) 377, 407
HindIII AAGCTT 1 cut(s) 848
HinfI GANTC 6 cut(s) 40, 282, 428, 556, 569, 574
HpaII CCGG 1 cut(s) 533
HphI GGTGA 4 cut(s) 172, 229, 502, 821
Hpy166II GTNNAC 1 cut(s) 543
Hpy188I TCNGA 6 cut(s) 24, 526, 604, 649, 771, 1019
Hpy8I GTNNAC 1 cut(s) 543
Hpy99I CGWCG 1 cut(s) 518
HpyAV CCTTC 1 cut(s) 678
HpyCH4III ACNGT 1 cut(s) 514
HpyCH4IV ACGT 2 cut(s) 164, 580
HpyCH4V TGCA 3 cut(s) 266, 464, 629
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 626, 673, 842
HpyF3I CTNAG 3 cut(s) 244, 360, 660
HpySE526I ACGT 2 cut(s) 164, 580
Hsp92I GRCGYC 1 cut(s) 407
Hsp92II CATG 3 cut(s) 376, 403, 767
HspAI GCGC 2 cut(s) 377, 407
KasI GGCGCC 1 cut(s) 406
KpnI GGTACC 1 cut(s) 726
Ksp22I TGATCA 2 cut(s) 771, 811
LmnI GCTCC 2 cut(s) 438, 876
Lsp1109I GCAGC 3 cut(s) 110, 613, 676
LweI GCATC 2 cut(s) 638, 956
MaeI CTAG 3 cut(s) 323, 330, 608
MaeII ACGT 2 cut(s) 164, 580
MboII GAAGA 4 cut(s) 360, 387, 430, 929
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 2 cut(s) 206, 756
MluCI AATT 5 cut(s) 150, 310, 561, 743, 821
Mly113I GGCGCC 1 cut(s) 407
MlyI GAGTC 4 cut(s) 49, 291, 437, 550
MseI TTAA 5 cut(s) 92, 149, 153, 294, 929
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 1 cut(s) 533
MspR9I CCNGG 3 cut(s) 53, 533, 943
MvaI CCWGG 2 cut(s) 53, 943
MvnI CGCG 1 cut(s) 379
MwoI GCNNNNNNNGC 4 cut(s) 263, 626, 673, 842
NarI GGCGCC 1 cut(s) 407
NciI CCSGG 1 cut(s) 533
NcoI CCATGG 2 cut(s) 372, 399
NlaIII CATG 3 cut(s) 376, 403, 767
NlaIV GGNNCC 3 cut(s) 408, 646, 724
NspV TTCGAA 1 cut(s) 276
PacI TTAATTAA 1 cut(s) 153
PagI TCATGA 1 cut(s) 763
PcsI WCGNNNNNNNCGW 3 cut(s) 237, 495, 510
PfeI GAWTC 2 cut(s) 569, 574
PfoI TCCNGGA 2 cut(s) 51, 531
PkrI GCNGC 8 cut(s) 125, 406, 520, 596, 628, 666, 784, 835
Ple19I CGATCG 1 cut(s) 880
PleI GAGTC 4 cut(s) 48, 290, 436, 550
PluTI GGCGCC 1 cut(s) 410
PpsI GAGTC 4 cut(s) 48, 290, 436, 550
Psp1406I AACGTT 1 cut(s) 580
Psp6I CCWGG 2 cut(s) 51, 941
PspGI CCWGG 2 cut(s) 51, 941
PspN4I GGNNCC 3 cut(s) 408, 646, 724
PspPI GGNCC 2 cut(s) 226, 645
PsuI RGATCY 1 cut(s) 19
PvuI CGATCG 1 cut(s) 880
RsaI GTAC 4 cut(s) 136, 440, 724, 1008
RsaNI GTAC 4 cut(s) 135, 439, 723, 1007
SaqAI TTAA 5 cut(s) 92, 149, 153, 294, 929
SatI GCNGC 8 cut(s) 124, 405, 519, 595, 627, 665, 783, 834
Sau96I GGNCC 2 cut(s) 226, 645
ScaI AGTACT 2 cut(s) 440, 1008
SchI GAGTC 4 cut(s) 49, 291, 437, 550
ScrFI CCNGG 3 cut(s) 53, 533, 943
SduI GDGCHC 2 cut(s) 206, 756
SetI ASST 8 cut(s) 125, 167, 196, 335, 479, 583, 852, 873
SfaNI GCATC 2 cut(s) 638, 956
SfoI GGCGCC 1 cut(s) 408
SfuI TTCGAA 1 cut(s) 276
SinI GGWCC 2 cut(s) 226, 645
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 5 cut(s) 150, 310, 561, 743, 821
SsiI CCGC 9 cut(s) 17, 379, 404, 519, 594, 597, 780, 783, 833
SspDI GGCGCC 1 cut(s) 406
SspMI CTAG 3 cut(s) 323, 330, 608
StyD4I CCNGG 3 cut(s) 51, 531, 941
StyI CCWWGG 2 cut(s) 372, 399
TaaI ACNGT 1 cut(s) 514
TaiI ACGT 2 cut(s) 167, 583
TasI AATT 5 cut(s) 150, 310, 561, 743, 821
TatI WGTACW 3 cut(s) 134, 438, 1006
TauI GCSGC 5 cut(s) 407, 521, 597, 785, 836
TfiI GAWTC 2 cut(s) 569, 574
Tru1I TTAA 5 cut(s) 92, 149, 153, 294, 929
Tru9I TTAA 5 cut(s) 92, 149, 153, 294, 929
TscAI CASTG 1 cut(s) 466
TseI GCWGC 3 cut(s) 123, 626, 664
TspDTI ATGAA 2 cut(s) 335, 843
TspRI CASTG 1 cut(s) 466
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 2 cut(s) 226, 645
XapI RAATTY 1 cut(s) 561
XspI CTAG 3 cut(s) 323, 330, 608
ZrmI AGTACT 2 cut(s) 440, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.