Rroxscaffold_4G00303350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005912
Physical Location & Seq
Reverse (-)
23780116 .. 23788566
8451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303350.1

Sequence Viewer

Length: 672 bp
ATGGATATGGGTGAGGATAAAGAAAATAATACATACCAGGCCATCACCCTCGCCGCCTCCGATTTTCCGGAGGAGTTCAGTCGCCAAAGGAGTCGAATTCTCGGTTCGATTCAGGGTTTGGGCGGCGGTGATGATCGGCGCTCCGATCTAGGGTTTGGTACAGGATCTGAAACTGGGTCGGAACAAAAGGCTGAGCCTTCAAAGCCATCGCTCAAAGTACGCATAAAGATCGTGAAGCGAAATGTTGATGGTACCTTGATCAAACCCAGTTCAGATGAAGCTAAGCCGATCAAGCCCTCTTCAGATGAGAATAAGTCGATCAAGCCGGAAACTTGTGTTGATAAAGTGGCAGTTTCCTGTGTTGGTGAAGCTAAATCGATCAAGCCGAAGCCGTGTTTGGATGAAGTGGCAATTGAGGCAAAGCTTCAGGCCACAAAGAGAAAGTTTCATGATCGATATGAAGAAATTGAGAACAGTAAGAGGCAGAGGAGGATACAAGTCTTGAATTATCTTCCCAAGCAAGACTTCAAGCTTGGATGCCCTACCAGTACTAAACCACAGGGGAAGAAACTTGGAAACCATTGTCTGCAGTCCTCGGTGAGAAGGCGGGTGGCAAATAAACTGGGTTTTCAGAGTTGTCAATTTAATAGGATAGTCAGTGCTACCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

24.76

Weight (kDa)

9.48

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 251
AccIII TCCGGA 1 cut(s) 67
AciI CCGC 4 cut(s) 54, 123, 126, 607
AclWI GGATC 1 cut(s) 172
AcsI RAATTY 1 cut(s) 96
AcuI CTGAAG 2 cut(s) 285, 410
AfaI GTAC 4 cut(s) 160, 219, 253, 550
AfiI CCNNNNNNNGG 1 cut(s) 150
AgsI TTSAA 3 cut(s) 201, 505, 529
AjnI CCWGG 1 cut(s) 36
AjuI GAANNNNNNNTTGG 4 cut(s) 380, 412, 509, 541
AluBI AGCT 4 cut(s) 281, 371, 424, 532
AluI AGCT 4 cut(s) 281, 371, 424, 532
AlwI GGATC 1 cut(s) 172
AlwNI CAGNNNCTG 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 67
AoxI GGCC 2 cut(s) 39, 429
ApoI RAATTY 1 cut(s) 96
Asp718I GGTACC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 141
AsuHPI GGTGA 5 cut(s) 23, 37, 140, 377, 610
BanI GGYRCC 1 cut(s) 251
BccI CCATC 3 cut(s) 50, 214, 242
BceAI ACGGC 1 cut(s) 376
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 1 cut(s) 38
BciVI GTATCC 1 cut(s) 486
BclI TGATCA 1 cut(s) 258
BfaI CTAG 1 cut(s) 149
BfmI CTRYAG 1 cut(s) 587
BfoI RGCGCY 1 cut(s) 142
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 2 cut(s) 54, 124
BlpI GCTNAGC 2 cut(s) 192, 282
BlsI GCNGC 2 cut(s) 55, 125
BmcAI AGTACT 1 cut(s) 550
Bme1390I CCNGG 1 cut(s) 38
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 38
BmrI ACTGGG 3 cut(s) 183, 261, 632
BmsI GCATC 1 cut(s) 527
BmuI ACTGGG 3 cut(s) 183, 261, 632
Bpu1102I GCTNAGC 2 cut(s) 192, 282
Bsa29I ATCGAT 2 cut(s) 377, 454
BsaJI CCNNGG 1 cut(s) 594
BsaWI WCCGGW 1 cut(s) 67
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse1I ACTGG 4 cut(s) 178, 267, 546, 627
BseAI TCCGGA 1 cut(s) 67
BseBI CCWGG 1 cut(s) 38
BseCI ATCGAT 2 cut(s) 377, 454
BseDI CCNNGG 1 cut(s) 594
BseGI GGATG 2 cut(s) 406, 542
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 4 cut(s) 178, 267, 546, 627
BseRI GAGGAG 2 cut(s) 86, 502
BshFI GGCC 2 cut(s) 41, 431
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 2 cut(s) 377, 454
BsiSI CCGG 2 cut(s) 68, 326
BslI CCNNNNNNNGG 1 cut(s) 150
BsnI GGCC 2 cut(s) 41, 431
Bsp13I TCCGGA 1 cut(s) 67
Bsp143I GATC 9 cut(s) 133, 145, 164, 228, 258, 288, 318, 378, 451
Bsp1720I GCTNAGC 2 cut(s) 192, 282
BspACI CCGC 4 cut(s) 54, 123, 126, 607
BspANI GGCC 2 cut(s) 41, 431
BspCNI CTCAG 1 cut(s) 184
BspDI ATCGAT 2 cut(s) 377, 454
BspEI TCCGGA 1 cut(s) 67
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 253
BspMAI CTGCAG 1 cut(s) 591
BspPI GGATC 1 cut(s) 172
BspT107I GGYRCC 1 cut(s) 251
BsrI ACTGG 4 cut(s) 178, 267, 546, 627
BssECI CCNNGG 1 cut(s) 594
BssMI GATC 9 cut(s) 133, 145, 164, 228, 258, 288, 318, 378, 451
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 1 cut(s) 304
BstDEI CTNAG 2 cut(s) 192, 282
BstF5I GGATG 2 cut(s) 406, 542
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 9 cut(s) 136, 148, 167, 231, 261, 291, 321, 381, 454
BstMBI GATC 9 cut(s) 133, 145, 164, 228, 258, 288, 318, 378, 451
BstMWI GCNNNNNNNGC 3 cut(s) 202, 292, 416
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 587
BstX2I RGATCY 1 cut(s) 164
BstYI RGATCY 1 cut(s) 164
Bsu15I ATCGAT 2 cut(s) 377, 454
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 2 cut(s) 41, 431
BsuTUI ATCGAT 2 cut(s) 377, 454
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 2 cut(s) 406, 542
BtsIMutI CAGTG 2 cut(s) 664, 667
CaiI CAGNNNCTG 1 cut(s) 167
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 141
ClaI ATCGAT 2 cut(s) 377, 454
Csp6I GTAC 4 cut(s) 159, 218, 252, 549
CviAII CATG 1 cut(s) 449
CviQI GTAC 4 cut(s) 159, 218, 252, 549
DdeI CTNAG 2 cut(s) 192, 282
DpnI GATC 9 cut(s) 135, 147, 166, 230, 260, 290, 320, 380, 453
DpnII GATC 9 cut(s) 133, 145, 164, 228, 258, 288, 318, 378, 451
Eam1104I CTCTTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 304
Eco57I CTGAAG 2 cut(s) 285, 410
EcoRI GAATTC 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 36
FaeI CATG 1 cut(s) 452
FaiI YATR 5 cut(s) 8, 34, 224, 450, 459
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FatI CATG 1 cut(s) 448
FauI CCCGC 1 cut(s) 600
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 2 cut(s) 54, 124
FokI GGATG 2 cut(s) 413, 549
Fsp4HI GCNGC 2 cut(s) 54, 124
FspBI CTAG 1 cut(s) 149
GlaI GCGC 1 cut(s) 140
GluI GCNGC 2 cut(s) 54, 124
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 2 cut(s) 41, 431
HapII CCGG 2 cut(s) 68, 326
HhaI GCGC 1 cut(s) 141
Hin1II CATG 1 cut(s) 452
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HindIII AAGCTT 2 cut(s) 422, 530
HinfI GANTC 2 cut(s) 91, 109
HpaII CCGG 2 cut(s) 68, 326
HphI GGTGA 5 cut(s) 23, 37, 140, 377, 610
Hpy188I TCNGA 7 cut(s) 61, 145, 169, 181, 274, 304, 633
Hpy188III TCNNGA 4 cut(s) 68, 232, 449, 502
HpyAV CCTTC 2 cut(s) 207, 597
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4V TGCA 1 cut(s) 589
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 292, 416
HpyF3I CTNAG 2 cut(s) 192, 282
Hsp92II CATG 1 cut(s) 452
HspAI GCGC 1 cut(s) 139
Kpn2I TCCGGA 1 cut(s) 67
KpnI GGTACC 1 cut(s) 255
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 9 cut(s) 133, 145, 164, 228, 258, 288, 318, 378, 451
LmnI GCTCC 1 cut(s) 146
LweI GCATC 1 cut(s) 527
MaeI CTAG 1 cut(s) 149
MalI GATC 9 cut(s) 135, 147, 166, 230, 260, 290, 320, 380, 453
MboI GATC 9 cut(s) 133, 145, 164, 228, 258, 288, 318, 378, 451
MboII GAAGA 4 cut(s) 291, 473, 503, 577
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 1 cut(s) 164
MluCI AATT 5 cut(s) 96, 411, 465, 505, 641
MlyI GAGTC 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 159
MroI TCCGGA 1 cut(s) 67
MseI TTAA 1 cut(s) 645
MspI CCGG 2 cut(s) 68, 326
MspR9I CCNGG 1 cut(s) 38
MunI CAATTG 1 cut(s) 411
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 202, 292, 416
NdeII GATC 9 cut(s) 133, 145, 164, 228, 258, 288, 318, 378, 451
NlaIII CATG 1 cut(s) 452
NlaIV GGNNCC 1 cut(s) 253
PagI TCATGA 1 cut(s) 448
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 383
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 2 cut(s) 55, 125
PleI GAGTC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 253
PstI CTGCAG 1 cut(s) 591
PstNI CAGNNNCTG 1 cut(s) 167
PsuI RGATCY 1 cut(s) 164
RsaI GTAC 4 cut(s) 160, 219, 253, 550
RsaNI GTAC 4 cut(s) 159, 218, 252, 549
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 2 cut(s) 54, 124
Sau3AI GATC 9 cut(s) 133, 145, 164, 228, 258, 288, 318, 378, 451
ScaI AGTACT 1 cut(s) 550
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 38
SetI ASST 5 cut(s) 257, 283, 373, 426, 534
SfaNI GCATC 1 cut(s) 527
SfcI CTRYAG 1 cut(s) 587
Sse9I AATT 5 cut(s) 96, 411, 465, 505, 641
SsiI CCGC 4 cut(s) 54, 123, 126, 607
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 476
TaqI TCGA 5 cut(s) 94, 107, 317, 377, 454
TasI AATT 5 cut(s) 96, 411, 465, 505, 641
TatI WGTACW 1 cut(s) 548
TauI GCSGC 2 cut(s) 56, 126
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TscAI CASTG 1 cut(s) 664
TspDTI ATGAA 4 cut(s) 291, 417, 437, 474
TspRI CASTG 1 cut(s) 664
XapI RAATTY 1 cut(s) 96
XspI CTAG 1 cut(s) 149
ZrmI AGTACT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.