Rmu_sc0020815.1_g000008

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020815.1
OG0005912
Physical Location & Seq
Reverse (-)
31593 .. 32246
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020815.1_g000008.1.cds

Sequence Viewer

Length: 654 bp
atggcgcgggcgatcccgttttcttccatggcggcgccgatccagaagaaaccagagtcgctccagtactggagggatttctttgccactgcaaacaactccacctccaccattttcgatattctcgaccacgccatcaccgtcgccgcctctgatttcccggaggagttcactcgccagaggactcgaattctcgattcgattcaacgtttgggtttgggcggcggttctgatctagggttttgtgctggtgctgcatctggaagttctgggtccgatcaaaaggctgagcagccttcaaaaccatcgctcaaaatacgcataaagatcgtgaagcgaaatgttgatggtaccctgatcgaaccagcgaaattgatcgagccctgttctcatgatctgatcaagcccgccgcaccctgtgttggcgttggtgttggtgatcaagcaaaattggatgaagcggctattgaggccaagcttcaggccacaaagagaaagctccacgatcgatatcaagaaatcgagaaccataagaggcagaggaggatacaagtcttaaaccatcttcccaggcaagacttcaggattggtcgatgccctgctaaacccagacttggaaaccattggaaaccagtactaacaatcagaacataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

217

Amino Acids

24.06

Weight (kDa)

9.75

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 350
AccB1I GGYRCC 2 cut(s) 34, 350
AccII CGCG 1 cut(s) 7
AciI CCGC 8 cut(s) 7, 32, 147, 222, 225, 408, 411, 461
AclI AACGTT 1 cut(s) 208
AclWI GGATC 2 cut(s) 7, 34
AcsI RAATTY 1 cut(s) 189
AcuI CTGAAG 2 cut(s) 464, 565
AcyI GRCGYC 1 cut(s) 35
AdeI CACNNNGTG 1 cut(s) 419
AfaI GTAC 3 cut(s) 68, 352, 636
AfiI CCNNNNNNNGG 2 cut(s) 422, 614
AgsI TTSAA 2 cut(s) 206, 300
AjnI CCWGG 1 cut(s) 569
AluBI AGCT 2 cut(s) 478, 499
AluI AGCT 2 cut(s) 478, 499
AlwI GGATC 2 cut(s) 7, 34
AoxI GGCC 2 cut(s) 471, 483
ApeKI GCWGC 2 cut(s) 254, 292
ApoI RAATTY 1 cut(s) 189
Asp718I GGTACC 1 cut(s) 350
AspLEI GCGC 2 cut(s) 7, 37
AspS9I GGNCC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 130, 449
AvaII GGWCC 1 cut(s) 273
BanI GGYRCC 2 cut(s) 34, 350
BanII GRGCYC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 241, 304
BccI CCATC 4 cut(s) 143, 313, 341, 570
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 1 cut(s) 571
BciVI GTATCC 1 cut(s) 540
BclI TGATCA 2 cut(s) 399, 439
BcnI CCSGG 1 cut(s) 161
BfaI CTAG 1 cut(s) 236
BfoI RGCGCY 1 cut(s) 38
BfuI GTATCC 1 cut(s) 540
BisI GCNGC 7 cut(s) 33, 147, 223, 255, 293, 411, 462
BlpI GCTNAGC 1 cut(s) 288
BlsI GCNGC 7 cut(s) 34, 148, 224, 256, 294, 412, 463
BmcAI AGTACT 2 cut(s) 68, 636
Bme1390I CCNGG 2 cut(s) 161, 571
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 3 cut(s) 36, 274, 352
BmrFI CCNGG 2 cut(s) 161, 571
BmsI GCATC 2 cut(s) 266, 584
BpmI CTGGAG 2 cut(s) 47, 91
Bpu1102I GCTNAGC 1 cut(s) 288
BpuMI CCSGG 1 cut(s) 161
Bsa29I ATCGAT 1 cut(s) 508
BsaBI GATNNNNATC 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 27, 569
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 614
Bse1I ACTGG 3 cut(s) 64, 74, 632
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 1 cut(s) 571
BseCI ATCGAT 1 cut(s) 508
BseDI CCNNGG 2 cut(s) 27, 569
BseGI GGATG 1 cut(s) 460
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 2 cut(s) 422, 614
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 3 cut(s) 64, 74, 632
BseRI GAGGAG 2 cut(s) 179, 556
BseXI GCAGC 2 cut(s) 241, 304
Bsh1236I CGCG 1 cut(s) 7
Bsh1285I CGRYCG 1 cut(s) 508
BshFI GGCC 2 cut(s) 473, 485
BshNI GGYRCC 2 cut(s) 34, 350
BshVI ATCGAT 1 cut(s) 508
BsiEI CGRYCG 1 cut(s) 508
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 2 cut(s) 422, 614
BsnI GGCC 2 cut(s) 473, 485
Bsp1286I GDGCHC 1 cut(s) 384
Bsp1720I GCTNAGC 1 cut(s) 288
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 8 cut(s) 7, 32, 147, 222, 225, 408, 411, 461
BspANI GGCC 2 cut(s) 473, 485
BspCNI CTCAG 1 cut(s) 280
BspDI ATCGAT 1 cut(s) 508
BspFNI CGCG 1 cut(s) 7
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 3 cut(s) 36, 274, 352
BspPI GGATC 2 cut(s) 7, 34
BspT107I GGYRCC 2 cut(s) 34, 350
BsrI ACTGG 3 cut(s) 64, 74, 632
BssECI CCNNGG 2 cut(s) 27, 569
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 571
Bst4CI ACNGT 1 cut(s) 142
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 9, 408
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 460
BstFNI CGCG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 2 cut(s) 7, 37
BstMCI CGRYCG 1 cut(s) 508
BstMWI GCNNNNNNNGC 2 cut(s) 254, 470
BstNI CCWGG 1 cut(s) 571
BstSCI CCNGG 2 cut(s) 159, 569
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 2 cut(s) 241, 304
Bsu15I ATCGAT 1 cut(s) 508
BsuI GTATCC 1 cut(s) 540
BsuRI GGCC 2 cut(s) 473, 485
BsuTUI ATCGAT 1 cut(s) 508
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 1 cut(s) 460
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 2 cut(s) 9, 408
CciI TCATGA 1 cut(s) 391
CfoI GCGC 2 cut(s) 7, 37
Cfr13I GGNCC 1 cut(s) 273
ClaI ATCGAT 1 cut(s) 508
Csp6I GTAC 3 cut(s) 67, 351, 635
CviAII CATG 2 cut(s) 28, 392
CviJI RGCY 9 cut(s) 287, 295, 382, 406, 464, 473, 478, 485, 499
CviKI_1 RGCY 9 cut(s) 287, 295, 382, 406, 464, 473, 478, 485, 499
CviQI GTAC 3 cut(s) 67, 351, 635
DdeI CTNAG 1 cut(s) 288
DinI GGCGCC 1 cut(s) 36
DraIII CACNNNGTG 1 cut(s) 419
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 384
Eco32I GATATC 1 cut(s) 512
Eco47I GGWCC 1 cut(s) 273
Eco57I CTGAAG 2 cut(s) 464, 565
EcoRI GAATTC 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 569
EcoRV GATATC 1 cut(s) 512
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 384
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 2 cut(s) 31, 395
FaiI YATR 5 cut(s) 29, 323, 393, 531, 652
FatI CATG 2 cut(s) 27, 391
FauI CCCGC 1 cut(s) 415
FbaI TGATCA 2 cut(s) 399, 439
Fnu4HI GCNGC 7 cut(s) 33, 147, 223, 255, 293, 411, 462
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 7 cut(s) 33, 147, 223, 255, 293, 411, 462
FspBI CTAG 1 cut(s) 236
GlaI GCGC 2 cut(s) 6, 36
GluI GCNGC 7 cut(s) 33, 147, 223, 255, 293, 411, 462
GsuI CTGGAG 2 cut(s) 47, 91
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 2 cut(s) 473, 485
HapII CCGG 1 cut(s) 161
HhaI GCGC 2 cut(s) 7, 37
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 2 cut(s) 31, 395
Hin6I GCGC 2 cut(s) 5, 35
HinP1I GCGC 2 cut(s) 5, 35
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 4 cut(s) 56, 184, 197, 202
HpaII CCGG 1 cut(s) 161
HphI GGTGA 2 cut(s) 130, 449
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 5 cut(s) 154, 232, 277, 399, 647
Hpy188III TCNNGA 9 cut(s) 43, 125, 194, 261, 331, 392, 515, 523, 583
Hpy8I GTNNAC 1 cut(s) 171
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 306
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 208
HpyCH4V TGCA 2 cut(s) 92, 257
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 470
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 208
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 2 cut(s) 31, 395
HspAI GCGC 2 cut(s) 5, 35
KasI GGCGCC 1 cut(s) 34
KpnI GGTACC 1 cut(s) 354
Ksp22I TGATCA 2 cut(s) 399, 439
LmnI GCTCC 2 cut(s) 66, 504
Lsp1109I GCAGC 2 cut(s) 241, 304
LweI GCATC 2 cut(s) 266, 584
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 208
MboII GAAGA 3 cut(s) 15, 58, 557
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 3 cut(s) 189, 371, 449
Mly113I GGCGCC 1 cut(s) 35
MlyI GAGTC 2 cut(s) 65, 178
MnlI CCTC 9 cut(s) 66, 115, 157, 160, 174, 463, 528, 534, 537
MseI TTAA 1 cut(s) 557
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 161, 571
MvaI CCWGG 1 cut(s) 571
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 2 cut(s) 254, 470
NarI GGCGCC 1 cut(s) 35
NciI CCSGG 1 cut(s) 161
NcoI CCATGG 1 cut(s) 27
NlaIII CATG 2 cut(s) 31, 395
NlaIV GGNNCC 3 cut(s) 36, 274, 352
PagI TCATGA 1 cut(s) 391
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 138
PfeI GAWTC 2 cut(s) 197, 202
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 7 cut(s) 34, 148, 224, 256, 294, 412, 463
Ple19I CGATCG 1 cut(s) 508
PleI GAGTC 2 cut(s) 64, 178
PluTI GGCGCC 1 cut(s) 38
PpsI GAGTC 2 cut(s) 64, 178
Psp1406I AACGTT 1 cut(s) 208
Psp6I CCWGG 1 cut(s) 569
PspGI CCWGG 1 cut(s) 569
PspN4I GGNNCC 3 cut(s) 36, 274, 352
PspPI GGNCC 1 cut(s) 273
PvuI CGATCG 1 cut(s) 508
RsaI GTAC 3 cut(s) 68, 352, 636
RsaNI GTAC 3 cut(s) 67, 351, 635
SaqAI TTAA 1 cut(s) 557
SatI GCNGC 7 cut(s) 33, 147, 223, 255, 293, 411, 462
Sau96I GGNCC 1 cut(s) 273
ScaI AGTACT 2 cut(s) 68, 636
SchI GAGTC 2 cut(s) 65, 178
ScrFI CCNGG 2 cut(s) 161, 571
SduI GDGCHC 1 cut(s) 384
SetI ASST 4 cut(s) 107, 211, 480, 501
SfaNI GCATC 2 cut(s) 266, 584
SfoI GGCGCC 1 cut(s) 36
SinI GGWCC 1 cut(s) 273
Sse9I AATT 3 cut(s) 189, 371, 449
SsiI CCGC 8 cut(s) 7, 32, 147, 222, 225, 408, 411, 461
SspDI GGCGCC 1 cut(s) 34
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 2 cut(s) 159, 569
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 1 cut(s) 211
TasI AATT 3 cut(s) 189, 371, 449
TatI WGTACW 2 cut(s) 66, 634
TauI GCSGC 5 cut(s) 35, 149, 225, 413, 464
TfiI GAWTC 2 cut(s) 197, 202
Tru1I TTAA 1 cut(s) 557
Tru9I TTAA 1 cut(s) 557
TscAI CASTG 1 cut(s) 94
TseI GCWGC 2 cut(s) 254, 292
TspDTI ATGAA 1 cut(s) 471
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 273
XapI RAATTY 1 cut(s) 189
XspI CTAG 1 cut(s) 236
ZrmI AGTACT 2 cut(s) 68, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.