Rh1BG208100

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0005912
Physical Location & Seq
Forward (+)
32612961 .. 32613656
696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG208100.1

Sequence Viewer

Length: 696 bp
ATGGCGACGATCCAGAAAACTGAGTCGCTGAATTACTGGAGGGATTTCTTTGCCACTGCAAACAACTCCACCATTTTCGATGTTCTCGACCACGCCATCACCCTCGCCGCCTCCTATTTTCCGGAGGAGTTCAGTCGCCAAAGGAGTCGAATTCTCAGTTCGATTCAGGGTTTGGGCGGCGGTGATGATCGGCGCTCCGATCTAGGGTTTGGTGGCGCATCTGAAACTGGGTCGGAACAAAAGGCTGAGCCTTCAAAGCCATCGCTCAAAGTACGCATAAAGATCGTGAAGCGAAACGTTGATGGTACCTTGATCAAACCCAGTTCAGATGAAGCTAAACCGATCAAGCCCTCTTCAGATGAGAATAAGTCGATCAAGCCGGAACCGTGTGTTGATAAAGTGGCACTTGATCGAGCGAAAATGATCAAGCCCCCACCCTGTGTTGGTGAAGCTAAATCGATCAAGCCGAAGCCGTATTTGGATGAAGTGGCAATTGAGGCAAAGCTTCAGGCCACAAAGAGAAAGTTTCATGATCGATATGAAGAAATTGAGAACAGTAAGAGGCAAAGGAGGATACAAGTCTTGAGTTATCTTCCCAAGCAAGACTTCAAGATTGGATGCCGTCCTAGTACTAAACCACAGGGGAAGAAACTTGGAAACCATTGTCTGCAATCCTCGCATAGGGTTCGTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

231

Amino Acids

25.92

Weight (kDa)

9.66

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 305
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 3 cut(s) 108, 177, 180
AclI AACGTT 1 cut(s) 297
AclWI GGATC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 150
AcuI CTGAAG 2 cut(s) 339, 491
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 3 cut(s) 273, 307, 631
AfiI CCNNNNNNNGG 3 cut(s) 204, 443, 681
AgsI TTSAA 2 cut(s) 255, 610
AjuI GAANNNNNNNTTGG 4 cut(s) 461, 493, 590, 622
AluBI AGCT 3 cut(s) 335, 452, 505
AluI AGCT 3 cut(s) 335, 452, 505
AlwI GGATC 1 cut(s) 4
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 1 cut(s) 510
ApoI RAATTY 1 cut(s) 150
Asp718I GGTACC 1 cut(s) 305
AspLEI GCGC 2 cut(s) 195, 218
AsuHPI GGTGA 3 cut(s) 91, 194, 458
BanI GGYRCC 1 cut(s) 305
BccI CCATC 3 cut(s) 104, 268, 296
BceAI ACGGC 2 cut(s) 457, 606
BcgI CGANNNNNNTGC 3 cut(s) 265, 299, 668
BciVI GTATCC 1 cut(s) 567
BclI TGATCA 2 cut(s) 312, 423
BfaI CTAG 2 cut(s) 203, 627
BfoI RGCGCY 1 cut(s) 196
BfuI GTATCC 1 cut(s) 567
BisI GCNGC 2 cut(s) 108, 178
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 2 cut(s) 109, 179
BmcAI AGTACT 1 cut(s) 631
BmiI GGNNCC 2 cut(s) 307, 384
BmrI ACTGGG 2 cut(s) 237, 315
BmsI GCATC 2 cut(s) 227, 608
BmuI ACTGGG 2 cut(s) 237, 315
BpmI CTGGAG 1 cut(s) 58
Bpu1102I GCTNAGC 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 604
Bsa29I ATCGAT 2 cut(s) 458, 535
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 443, 681
Bse1I ACTGG 3 cut(s) 41, 232, 321
BseAI TCCGGA 1 cut(s) 121
BseCI ATCGAT 2 cut(s) 458, 535
BseGI GGATG 2 cut(s) 487, 623
BseLI CCNNNNNNNGG 3 cut(s) 204, 443, 681
BseMII CTCAG 3 cut(s) 12, 169, 237
BseNI ACTGG 3 cut(s) 41, 232, 321
BseRI GAGGAG 1 cut(s) 140
BshFI GGCC 1 cut(s) 512
BshNI GGYRCC 1 cut(s) 305
BshVI ATCGAT 2 cut(s) 458, 535
BsiSI CCGG 2 cut(s) 122, 380
BslI CCNNNNNNNGG 3 cut(s) 204, 443, 681
BsnI GGCC 1 cut(s) 512
Bsp13I TCCGGA 1 cut(s) 121
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 3 cut(s) 108, 177, 180
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 3 cut(s) 13, 168, 238
BspDI ATCGAT 2 cut(s) 458, 535
BspEI TCCGGA 1 cut(s) 121
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 2 cut(s) 307, 384
BspPI GGATC 1 cut(s) 4
BspT107I GGYRCC 1 cut(s) 305
BsrI ACTGG 3 cut(s) 41, 232, 321
Bst4CI ACNGT 2 cut(s) 387, 557
Bst6I CTCTTC 1 cut(s) 358
BstDEI CTNAG 3 cut(s) 21, 155, 246
BstENI CCTNNNNNAGG 1 cut(s) 679
BstF5I GGATG 2 cut(s) 487, 623
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 2 cut(s) 195, 218
BstMWI GCNNNNNNNGC 3 cut(s) 256, 497, 676
Bsu15I ATCGAT 2 cut(s) 458, 535
BsuI GTATCC 1 cut(s) 567
BsuRI GGCC 1 cut(s) 512
BsuTUI ATCGAT 2 cut(s) 458, 535
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 487, 623
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 54
CciI TCATGA 1 cut(s) 529
CfoI GCGC 2 cut(s) 195, 218
ClaI ATCGAT 2 cut(s) 458, 535
Csp6I GTAC 3 cut(s) 272, 306, 630
CviAII CATG 1 cut(s) 530
CviQI GTAC 3 cut(s) 272, 306, 630
DdeI CTNAG 3 cut(s) 21, 155, 246
DraIII CACNNNGTG 1 cut(s) 440
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Eco57I CTGAAG 2 cut(s) 339, 491
EcoNI CCTNNNNNAGG 1 cut(s) 679
EcoRI GAATTC 1 cut(s) 150
FaeI CATG 1 cut(s) 533
FaiI YATR 6 cut(s) 278, 531, 540, 681, 692, 694
FalI AAGNNNNNCTT 4 cut(s) 390, 422, 590, 622
FatI CATG 1 cut(s) 529
FbaI TGATCA 2 cut(s) 312, 423
Fnu4HI GCNGC 2 cut(s) 108, 178
FokI GGATG 2 cut(s) 494, 630
Fsp4HI GCNGC 2 cut(s) 108, 178
FspBI CTAG 2 cut(s) 203, 627
GlaI GCGC 2 cut(s) 194, 217
GluI GCNGC 2 cut(s) 108, 178
GsuI CTGGAG 1 cut(s) 58
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 1 cut(s) 512
HapII CCGG 2 cut(s) 122, 380
HhaI GCGC 2 cut(s) 195, 218
Hin1II CATG 1 cut(s) 533
Hin6I GCGC 2 cut(s) 193, 216
HinP1I GCGC 2 cut(s) 193, 216
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 3 cut(s) 23, 145, 163
HpaII CCGG 2 cut(s) 122, 380
HphI GGTGA 3 cut(s) 91, 194, 458
Hpy188I TCNGA 5 cut(s) 199, 223, 235, 328, 358
Hpy188III TCNNGA 7 cut(s) 13, 86, 122, 286, 530, 583, 610
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 261
HpyCH4III ACNGT 2 cut(s) 387, 557
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 2 cut(s) 59, 670
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 497, 676
HpyF3I CTNAG 3 cut(s) 21, 155, 246
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 1 cut(s) 533
HspAI GCGC 2 cut(s) 193, 216
Kpn2I TCCGGA 1 cut(s) 121
KpnI GGTACC 1 cut(s) 309
Ksp22I TGATCA 2 cut(s) 312, 423
LmnI GCTCC 1 cut(s) 200
LweI GCATC 2 cut(s) 227, 608
MaeI CTAG 2 cut(s) 203, 627
MaeII ACGT 1 cut(s) 297
MboII GAAGA 4 cut(s) 345, 554, 584, 658
MfeI CAATTG 1 cut(s) 492
MluCI AATT 4 cut(s) 31, 150, 492, 546
MlyI GAGTC 2 cut(s) 32, 154
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 9 cut(s) 33, 113, 118, 121, 361, 490, 555, 564, 685
MroI TCCGGA 1 cut(s) 121
MspI CCGG 2 cut(s) 122, 380
MunI CAATTG 1 cut(s) 492
MwoI GCNNNNNNNGC 3 cut(s) 256, 497, 676
NlaIII CATG 1 cut(s) 533
NlaIV GGNNCC 2 cut(s) 307, 384
PagI TCATGA 1 cut(s) 529
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 464
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 2 cut(s) 109, 179
PleI GAGTC 2 cut(s) 31, 153
PpsI GAGTC 2 cut(s) 31, 153
Psp1406I AACGTT 1 cut(s) 297
PspN4I GGNNCC 2 cut(s) 307, 384
RsaI GTAC 3 cut(s) 273, 307, 631
RsaNI GTAC 3 cut(s) 272, 306, 630
SatI GCNGC 2 cut(s) 108, 178
ScaI AGTACT 1 cut(s) 631
SchI GAGTC 2 cut(s) 32, 154
SetI ASST 5 cut(s) 300, 311, 337, 454, 507
SfaNI GCATC 2 cut(s) 227, 608
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
Sse9I AATT 4 cut(s) 31, 150, 492, 546
SsiI CCGC 3 cut(s) 108, 177, 180
SspMI CTAG 2 cut(s) 203, 627
TaaI ACNGT 2 cut(s) 387, 557
TaiI ACGT 1 cut(s) 300
TaqI TCGA 8 cut(s) 78, 87, 148, 161, 371, 412, 458, 535
TasI AATT 4 cut(s) 31, 150, 492, 546
TatI WGTACW 1 cut(s) 629
TauI GCSGC 2 cut(s) 110, 180
TfiI GAWTC 1 cut(s) 163
TscAI CASTG 1 cut(s) 61
TspDTI ATGAA 4 cut(s) 345, 498, 518, 555
TspRI CASTG 1 cut(s) 61
XagI CCTNNNNNAGG 1 cut(s) 679
XapI RAATTY 1 cut(s) 150
XspI CTAG 2 cut(s) 203, 627
ZrmI AGTACT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.