Rh1BG207800

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0005912
Physical Location & Seq
Forward (+)
32592593 .. 32593345
753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG207800.1

Sequence Viewer

Length: 753 bp
ATGGCGACGATCCAGAAAACTGAGTCGCTGAATTACTGGAGGGATTTCTTTGCCACTGCAAACAACTCCACCATTTTCGATGTTCTCGACCACGCCATCACCCTCGCCGCCTCCGATTTTCCGGAGGAGTTCAGTCGCCAAAGGAGTCGAATTCTCGGTTCGATTCAGGGTTTGGGCGGCGGTGATGATCGGCGCTCCGATCTAGGGTTTGGTGGCGCATCTGAAACTGGGTCGGAACAAAAGGCTGAGCCTCCAAAGCCATCGCTCAAAGTACGCATAAAGATCGTGAAACGAAACGTTGATGGTTCCTTGATCAAACCCAGTTCAGATGAAGCTAAGCCGATCAAGCCCTCTTCAGATGAGAATAAGTCGATCAAGCCGGAACCGTGTGTTGATAAAGTGGCACTTGATCGAGCGAAAATGATCAAGCCCCCACCCTGTGTTGGTGAAGCTAAATCGATCAAGCCGAAGCCGTGTTTGGATGAAGTGGCAATTGAGGCAAAGCTTCAGGCCACAAAGAGAAAGTTTCATGATCGATATGAAGAAATTGAGAACAGTAAGAGGCAGAGGAGGATACAAGTCTTGAATTATCTTCCCAACCAAGACTTCAAGCTTGGATGCCCTACCAGTACTAAACCACAGGGGAAGAAACTTGGAAACCATTGTCTACAGTCCTCGGCGAGAAGGCGGCTGGCAAATAAACTGGGTTTTCAGAGTTTTCAAAGTAATAGGATAGTCAGTGCTACCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

250

Amino Acids

27.82

Weight (kDa)

9.64

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 667
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 4 cut(s) 108, 177, 180, 688
AclI AACGTT 1 cut(s) 297
AclWI GGATC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 150
AcuI CTGAAG 2 cut(s) 339, 491
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 2 cut(s) 273, 631
AfiI CCNNNNNNNGG 2 cut(s) 204, 443
AgsI TTSAA 3 cut(s) 586, 610, 722
AjuI GAANNNNNNNTTGG 4 cut(s) 461, 493, 590, 622
AluBI AGCT 4 cut(s) 335, 452, 505, 613
AluI AGCT 4 cut(s) 335, 452, 505, 613
AlwI GGATC 1 cut(s) 4
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 1 cut(s) 510
ApoI RAATTY 1 cut(s) 150
AspLEI GCGC 2 cut(s) 195, 218
AsuHPI GGTGA 3 cut(s) 91, 194, 458
BccI CCATC 3 cut(s) 104, 268, 296
BceAI ACGGC 1 cut(s) 457
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BciVI GTATCC 1 cut(s) 567
BclI TGATCA 2 cut(s) 312, 423
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 668
BfoI RGCGCY 1 cut(s) 196
BfuI GTATCC 1 cut(s) 567
BisI GCNGC 3 cut(s) 108, 178, 689
BlpI GCTNAGC 2 cut(s) 246, 336
BlsI GCNGC 3 cut(s) 109, 179, 690
BmcAI AGTACT 1 cut(s) 631
BmiI GGNNCC 2 cut(s) 307, 384
BmrI ACTGGG 3 cut(s) 237, 315, 713
BmsI GCATC 2 cut(s) 227, 608
BmuI ACTGGG 3 cut(s) 237, 315, 713
BpmI CTGGAG 1 cut(s) 58
Bpu1102I GCTNAGC 2 cut(s) 246, 336
Bsa29I ATCGAT 2 cut(s) 458, 535
BsaJI CCNNGG 1 cut(s) 675
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 443
Bse1I ACTGG 5 cut(s) 41, 232, 321, 627, 708
BseAI TCCGGA 1 cut(s) 121
BseCI ATCGAT 2 cut(s) 458, 535
BseDI CCNNGG 1 cut(s) 675
BseGI GGATG 2 cut(s) 487, 623
BseLI CCNNNNNNNGG 2 cut(s) 204, 443
BseMII CTCAG 2 cut(s) 12, 237
BseNI ACTGG 5 cut(s) 41, 232, 321, 627, 708
BseRI GAGGAG 2 cut(s) 140, 583
BshFI GGCC 1 cut(s) 512
BshVI ATCGAT 2 cut(s) 458, 535
BsiSI CCGG 2 cut(s) 122, 380
BslI CCNNNNNNNGG 2 cut(s) 204, 443
BsnI GGCC 1 cut(s) 512
Bsp13I TCCGGA 1 cut(s) 121
Bsp1720I GCTNAGC 2 cut(s) 246, 336
BspACI CCGC 4 cut(s) 108, 177, 180, 688
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 2 cut(s) 13, 238
BspDI ATCGAT 2 cut(s) 458, 535
BspEI TCCGGA 1 cut(s) 121
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 2 cut(s) 307, 384
BspPI GGATC 1 cut(s) 4
BsrI ACTGG 5 cut(s) 41, 232, 321, 627, 708
BssECI CCNNGG 1 cut(s) 675
Bst4CI ACNGT 3 cut(s) 387, 557, 672
Bst6I CTCTTC 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 693
BstDEI CTNAG 3 cut(s) 21, 246, 336
BstF5I GGATG 2 cut(s) 487, 623
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 2 cut(s) 195, 218
BstMWI GCNNNNNNNGC 3 cut(s) 256, 346, 497
BstSFI CTRYAG 1 cut(s) 668
Bsu15I ATCGAT 2 cut(s) 458, 535
BsuI GTATCC 1 cut(s) 567
BsuRI GGCC 1 cut(s) 512
BsuTUI ATCGAT 2 cut(s) 458, 535
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 487, 623
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 3 cut(s) 54, 745, 748
Cac8I GCNNGC 1 cut(s) 693
CciI TCATGA 1 cut(s) 529
CfoI GCGC 2 cut(s) 195, 218
ClaI ATCGAT 2 cut(s) 458, 535
Csp6I GTAC 2 cut(s) 272, 630
CviAII CATG 1 cut(s) 530
CviQI GTAC 2 cut(s) 272, 630
DdeI CTNAG 3 cut(s) 21, 246, 336
DraIII CACNNNGTG 1 cut(s) 440
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Eco57I CTGAAG 2 cut(s) 339, 491
EcoRI GAATTC 1 cut(s) 150
FaeI CATG 1 cut(s) 533
FaiI YATR 3 cut(s) 278, 531, 540
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 1 cut(s) 529
FbaI TGATCA 2 cut(s) 312, 423
FblI GTMKAC 1 cut(s) 667
Fnu4HI GCNGC 3 cut(s) 108, 178, 689
FokI GGATG 2 cut(s) 494, 630
Fsp4HI GCNGC 3 cut(s) 108, 178, 689
FspBI CTAG 1 cut(s) 203
GlaI GCGC 2 cut(s) 194, 217
GluI GCNGC 3 cut(s) 108, 178, 689
GsuI CTGGAG 1 cut(s) 58
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 1 cut(s) 512
HapII CCGG 2 cut(s) 122, 380
HhaI GCGC 2 cut(s) 195, 218
Hin1II CATG 1 cut(s) 533
Hin6I GCGC 2 cut(s) 193, 216
HinP1I GCGC 2 cut(s) 193, 216
HindIII AAGCTT 2 cut(s) 503, 611
HinfI GANTC 3 cut(s) 23, 145, 163
HpaII CCGG 2 cut(s) 122, 380
HphI GGTGA 3 cut(s) 91, 194, 458
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 7 cut(s) 115, 199, 223, 235, 328, 358, 714
Hpy188III TCNNGA 6 cut(s) 13, 86, 122, 286, 530, 583
Hpy8I GTNNAC 1 cut(s) 668
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 678
HpyCH4III ACNGT 3 cut(s) 387, 557, 672
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 346, 497
HpyF3I CTNAG 3 cut(s) 21, 246, 336
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 1 cut(s) 533
HspAI GCGC 2 cut(s) 193, 216
Kpn2I TCCGGA 1 cut(s) 121
Ksp22I TGATCA 2 cut(s) 312, 423
LmnI GCTCC 1 cut(s) 200
LweI GCATC 2 cut(s) 227, 608
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 297
MboII GAAGA 4 cut(s) 345, 554, 584, 658
MfeI CAATTG 1 cut(s) 492
MluCI AATT 5 cut(s) 31, 150, 492, 546, 586
MlyI GAGTC 2 cut(s) 32, 154
MmeI TCCRAC 1 cut(s) 213
MroI TCCGGA 1 cut(s) 121
MspI CCGG 2 cut(s) 122, 380
MunI CAATTG 1 cut(s) 492
MwoI GCNNNNNNNGC 3 cut(s) 256, 346, 497
NlaIII CATG 1 cut(s) 533
NlaIV GGNNCC 2 cut(s) 307, 384
NmeAIII GCCGAG 1 cut(s) 656
PagI TCATGA 1 cut(s) 529
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 111, 464
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 3 cut(s) 109, 179, 690
PleI GAGTC 2 cut(s) 31, 153
PpsI GAGTC 2 cut(s) 31, 153
Psp1406I AACGTT 1 cut(s) 297
PspN4I GGNNCC 2 cut(s) 307, 384
RsaI GTAC 2 cut(s) 273, 631
RsaNI GTAC 2 cut(s) 272, 630
SatI GCNGC 3 cut(s) 108, 178, 689
ScaI AGTACT 1 cut(s) 631
SchI GAGTC 2 cut(s) 32, 154
SetI ASST 5 cut(s) 300, 337, 454, 507, 615
SfaNI GCATC 2 cut(s) 227, 608
SfcI CTRYAG 1 cut(s) 668
Sse9I AATT 5 cut(s) 31, 150, 492, 546, 586
SsiI CCGC 4 cut(s) 108, 177, 180, 688
SspMI CTAG 1 cut(s) 203
TaaI ACNGT 3 cut(s) 387, 557, 672
TaiI ACGT 1 cut(s) 300
TaqI TCGA 8 cut(s) 78, 87, 148, 161, 371, 412, 458, 535
TasI AATT 5 cut(s) 31, 150, 492, 546, 586
TatI WGTACW 1 cut(s) 629
TauI GCSGC 3 cut(s) 110, 180, 691
TfiI GAWTC 1 cut(s) 163
TscAI CASTG 2 cut(s) 61, 745
TspDTI ATGAA 4 cut(s) 345, 498, 518, 555
TspRI CASTG 2 cut(s) 61, 745
XapI RAATTY 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 667
XspI CTAG 1 cut(s) 203
ZrmI AGTACT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.