Rh1AG237600

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005912
Physical Location & Seq
Forward (+)
44724522 .. 44725596
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG237600.1

Sequence Viewer

Length: 753 bp
ATGGCGACGATCCAGAAAAATGAGTCGCTGAATTACTGGAGGGATTTCTTTGCCACTGCAAACAACTCCACCATTTTCGATGTTCTCGACCACGCCATCACCCTCGCCGCCTCCGATTTTCCGGAGGAGTTCAGTCGCCAAAGGAGTCGAATTCTCGGTTCGATTCAGGGTTTGGGTGGCGGTGATGATCGACGCTCCGATCTAGGGTTTGGTACAGCATCTGAAACTGGGTCGGAACAAAAGGCTGAGCCTTCAAAGCCATCGCTCAAAGTACTCATAAAGATCGTGAAACGAAACGTTGATGGTTCCTTGATCATACCCAGTTCAGATGAAGCTAAGCCGATCAAGCCCTCTTCAGATGAGAATAAGTCGATCAAGCAGGAAACGTGTGTTGATAAAGTGGCACTTGATCGAGCGAAAATGATCAAGCCCCCACCCTGTGTTGGTGAAGCTAAATCGATCAAGCCGAAGCCGTGTTTGGATGAAGTGGCAATTGAGGCAAAGCTTCTGGCCACAAAGAGAAAGTTTCATGATCGATATGAAGAAATTGAGAACAGTAAGAAGCAGAGGAGGATACAAGTCTTGAACTATCTTCCCAACCAAGACTTCAAGCTTGGATGCCCTACCAGTACTAAACCACAGGGGAAGAAACTTGGAAACCATTGTCTACAGTCCTCGGCGAGAAGGCGGGTGGCAAATAAACTAGGTTTTCAGAGTTTTCAAAGCAATAGGATAGTCAGTGCTGCCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

250

Amino Acids

27.75

Weight (kDa)

9.51

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 667
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 3 cut(s) 108, 180, 688
AclI AACGTT 1 cut(s) 297
AclWI GGATC 1 cut(s) 4
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 1 cut(s) 150
AcuI CTGAAG 1 cut(s) 339
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 3 cut(s) 214, 273, 631
AfiI CCNNNNNNNGG 2 cut(s) 204, 443
AflIII ACRYGT 1 cut(s) 386
AgsI TTSAA 4 cut(s) 255, 586, 610, 722
AjuI GAANNNNNNNTTGG 4 cut(s) 461, 493, 590, 622
AluBI AGCT 4 cut(s) 335, 452, 505, 613
AluI AGCT 4 cut(s) 335, 452, 505, 613
AlwI GGATC 1 cut(s) 4
AlwNI CAGNNNCTG 1 cut(s) 221
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 1 cut(s) 743
ApoI RAATTY 1 cut(s) 150
AsuHPI GGTGA 3 cut(s) 91, 194, 458
BalI TGGCCA 1 cut(s) 512
BbvI GCAGC 1 cut(s) 730
BccI CCATC 3 cut(s) 104, 268, 296
BceAI ACGGC 1 cut(s) 457
BciVI GTATCC 1 cut(s) 567
BclI TGATCA 2 cut(s) 312, 423
BfaI CTAG 2 cut(s) 203, 704
BfmI CTRYAG 1 cut(s) 668
BfuI GTATCC 1 cut(s) 567
BisI GCNGC 2 cut(s) 108, 744
BlpI GCTNAGC 2 cut(s) 246, 336
BlsI GCNGC 2 cut(s) 109, 745
BmcAI AGTACT 2 cut(s) 273, 631
BmiI GGNNCC 1 cut(s) 307
BmrI ACTGGG 2 cut(s) 237, 315
BmsI GCATC 2 cut(s) 227, 608
BmuI ACTGGG 2 cut(s) 237, 315
BpmI CTGGAG 1 cut(s) 58
Bpu1102I GCTNAGC 2 cut(s) 246, 336
Bsa29I ATCGAT 2 cut(s) 458, 535
BsaJI CCNNGG 1 cut(s) 675
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 443
Bse1I ACTGG 4 cut(s) 41, 232, 321, 627
BseAI TCCGGA 1 cut(s) 121
BseCI ATCGAT 2 cut(s) 458, 535
BseDI CCNNGG 1 cut(s) 675
BseGI GGATG 2 cut(s) 487, 623
BseLI CCNNNNNNNGG 2 cut(s) 204, 443
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 4 cut(s) 41, 232, 321, 627
BseRI GAGGAG 2 cut(s) 140, 583
BseXI GCAGC 1 cut(s) 730
BshFI GGCC 1 cut(s) 512
BshVI ATCGAT 2 cut(s) 458, 535
BsiSI CCGG 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 204, 443
BsnI GGCC 1 cut(s) 512
Bsp13I TCCGGA 1 cut(s) 121
Bsp1720I GCTNAGC 2 cut(s) 246, 336
BspACI CCGC 3 cut(s) 108, 180, 688
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 1 cut(s) 238
BspDI ATCGAT 2 cut(s) 458, 535
BspEI TCCGGA 1 cut(s) 121
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 1 cut(s) 307
BspPI GGATC 1 cut(s) 4
BsrI ACTGG 4 cut(s) 41, 232, 321, 627
BssECI CCNNGG 1 cut(s) 675
Bst4CI ACNGT 2 cut(s) 557, 672
Bst6I CTCTTC 1 cut(s) 358
BstDEI CTNAG 2 cut(s) 246, 336
BstF5I GGATG 2 cut(s) 487, 623
BstMWI GCNNNNNNNGC 3 cut(s) 256, 346, 497
BstSFI CTRYAG 1 cut(s) 668
BstV1I GCAGC 1 cut(s) 730
Bsu15I ATCGAT 2 cut(s) 458, 535
BsuI GTATCC 1 cut(s) 567
BsuRI GGCC 1 cut(s) 512
BsuTUI ATCGAT 2 cut(s) 458, 535
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 487, 623
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 3 cut(s) 54, 745, 748
CaiI CAGNNNCTG 1 cut(s) 221
CciI TCATGA 1 cut(s) 529
ClaI ATCGAT 2 cut(s) 458, 535
CseI GACGC 1 cut(s) 201
Csp6I GTAC 3 cut(s) 213, 272, 630
CviAII CATG 1 cut(s) 530
CviQI GTAC 3 cut(s) 213, 272, 630
DdeI CTNAG 2 cut(s) 246, 336
DraIII CACNNNGTG 1 cut(s) 440
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 339
EcoRI GAATTC 1 cut(s) 150
FaeI CATG 1 cut(s) 533
FaiI YATR 4 cut(s) 278, 317, 531, 540
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 1 cut(s) 529
FauI CCCGC 1 cut(s) 681
FbaI TGATCA 2 cut(s) 312, 423
FblI GTMKAC 1 cut(s) 667
Fnu4HI GCNGC 2 cut(s) 108, 744
FokI GGATG 2 cut(s) 494, 630
Fsp4HI GCNGC 2 cut(s) 108, 744
FspBI CTAG 2 cut(s) 203, 704
GluI GCNGC 2 cut(s) 108, 744
GsuI CTGGAG 1 cut(s) 58
HaeIII GGCC 1 cut(s) 512
HapII CCGG 1 cut(s) 122
HgaI GACGC 1 cut(s) 201
Hin1II CATG 1 cut(s) 533
HindIII AAGCTT 2 cut(s) 503, 611
HinfI GANTC 3 cut(s) 23, 145, 163
HpaII CCGG 1 cut(s) 122
HphI GGTGA 3 cut(s) 91, 194, 458
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 7 cut(s) 115, 199, 223, 235, 328, 358, 714
Hpy188III TCNNGA 6 cut(s) 13, 86, 122, 286, 530, 583
Hpy8I GTNNAC 1 cut(s) 668
Hpy99I CGWCG 2 cut(s) 10, 195
HpyAV CCTTC 2 cut(s) 261, 678
HpyCH4III ACNGT 2 cut(s) 557, 672
HpyCH4IV ACGT 2 cut(s) 297, 386
HpyCH4V TGCA 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 346, 497
HpyF3I CTNAG 2 cut(s) 246, 336
HpySE526I ACGT 2 cut(s) 297, 386
Hsp92II CATG 1 cut(s) 533
Kpn2I TCCGGA 1 cut(s) 121
Ksp22I TGATCA 2 cut(s) 312, 423
LmnI GCTCC 1 cut(s) 200
Lsp1109I GCAGC 1 cut(s) 730
LweI GCATC 2 cut(s) 227, 608
MaeI CTAG 2 cut(s) 203, 704
MaeII ACGT 2 cut(s) 297, 386
MboII GAAGA 4 cut(s) 345, 554, 584, 658
MfeI CAATTG 1 cut(s) 492
MlsI TGGCCA 1 cut(s) 512
MluCI AATT 4 cut(s) 31, 150, 492, 546
MluNI TGGCCA 1 cut(s) 512
MlyI GAGTC 2 cut(s) 32, 154
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 9 cut(s) 33, 113, 118, 121, 361, 490, 561, 564, 685
Mox20I TGGCCA 1 cut(s) 512
MroI TCCGGA 1 cut(s) 121
MscI TGGCCA 1 cut(s) 512
Msp20I TGGCCA 1 cut(s) 512
MspI CCGG 1 cut(s) 122
MunI CAATTG 1 cut(s) 492
MwoI GCNNNNNNNGC 3 cut(s) 256, 346, 497
NlaIII CATG 1 cut(s) 533
NlaIV GGNNCC 1 cut(s) 307
NmeAIII GCCGAG 1 cut(s) 656
PagI TCATGA 1 cut(s) 529
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 111, 464
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 2 cut(s) 109, 745
PleI GAGTC 2 cut(s) 31, 153
PpsI GAGTC 2 cut(s) 31, 153
Psp1406I AACGTT 1 cut(s) 297
PspN4I GGNNCC 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 221
RsaI GTAC 3 cut(s) 214, 273, 631
RsaNI GTAC 3 cut(s) 213, 272, 630
SatI GCNGC 2 cut(s) 108, 744
ScaI AGTACT 2 cut(s) 273, 631
SchI GAGTC 2 cut(s) 32, 154
SetI ASST 7 cut(s) 300, 337, 389, 454, 507, 615, 709
SfaNI GCATC 2 cut(s) 227, 608
SfcI CTRYAG 1 cut(s) 668
Sse9I AATT 4 cut(s) 31, 150, 492, 546
SsiI CCGC 3 cut(s) 108, 180, 688
SspMI CTAG 2 cut(s) 203, 704
TaaI ACNGT 2 cut(s) 557, 672
TaiI ACGT 2 cut(s) 300, 389
TaqI TCGA 9 cut(s) 78, 87, 148, 161, 190, 371, 412, 458, 535
TasI AATT 4 cut(s) 31, 150, 492, 546
TatI WGTACW 2 cut(s) 271, 629
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 1 cut(s) 163
TscAI CASTG 2 cut(s) 61, 745
TseI GCWGC 1 cut(s) 743
TspDTI ATGAA 4 cut(s) 345, 498, 518, 555
TspRI CASTG 2 cut(s) 61, 745
XapI RAATTY 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 667
XspI CTAG 2 cut(s) 203, 704
ZrmI AGTACT 2 cut(s) 273, 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.