FvH4_4g24410

Ankyrin repeats (3 copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002201
Physical Location & Seq
Reverse (-)
26591626 .. 26596773
5148 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24410.t1

Sequence Viewer

Length: 1986 bp
ATGCCGCCGTCGTACTTCCCTCTCCGGTGGGAAAGCACCGGAGACCAATGGTGGTACGCGTCGCCTATTGACTGGGCAGCAGCCAACGGCCACTACGACCTCGTCAGCGAGCTTCTTCACCTCGACACCAACCTCCTCATAAAGCTGACGTCCCTCCGCCGCATCCGCCGCCTCGAAACCGTGTGGGACGACGAGGCTCACTTCAGTGACGTGGCGAAATGCCGCTCTCAAGTCGCCAAGAGGCTTCTCCACGAGTGCCAGACGCAGAGTGGCCACAACTCTCTGATCCGGGCCGGCTACGGCGGCTGGCTGCTCTACACCGCCGCCTCAGCCGGTGACGTGGCGTTTGTTAAAGAGTTGTTGCAAAGAGACCCTCTTTTGGTGTTTGGGGAGGGAGAGTATGGTGTGACTGATATCTTTTACGCTGCCGCTAGGAGTAGGAATGGTGAGGTTTTCAGGGTGCTGCTAGACTTCTCGGTGTCGCCGAGGTTTGGTGATCGCAATGGGGAGGTGGTGGAGAGTAGGTCGGAGTTCAAGTGGGAGATGATGAACCGGGCGGTTCATGCGGCGGCGAGAGGTGGAAATCTAGAGGTTTTGAGGGAGTTGGTTGGGGGATGTGGTGATGTTTTGGCTTATAGGGATGCTCAGGGCTCTACTGTCTTGCATACTGCCTCTGGTAGAGGGCAGATTGAGGTGGTTAAGTATCTGATAGCATCCTTTGATATCAGCACCTCTGTAGATAGTCAAGGGAACACAGCTCTACATGTTGCCGCTTACAGGGGTCACTTGGCTGTGGTGGAAGCGCTAATTGGTGCATCTCCCTCGCTAGCCTCTTTATCGAACTACTATGGAGATACATTTCTGCATATGGCAGTGGCTGGTTTCCGAGCTCCTGGTTTCCGGAGAGTGGACAAACAGATTGAGCTCATCAAGCAATTAGTATGCGGTGATATTGTGAACATGCAGGACATCATTAATGATAGGAATAACAATGGAAGAACTGCTCTTCACATAGCTGTAAGTGAGAACATTCAGTCTAGTGTAGTGGAACTCCTCATGACTGTTCCATCAATAGATCTGAACATCCGTGATGGTGATGGCATGACGCCGCTAGATATTCTTAAGCAAAGGCCAAAGTCACCGTCTTCTGAACTTTTGATCAAGCAGTTGATTTCAGCTGGAGGCATCTCCAAGTGTCAAGACCATAAAGCAAGGAGTGCCATTGTTTCTCATTTGAGAATGCATGGTATTGGGAATAGTCCTGGAACCTCTTTCCGAATCCCTGATGCAGAGATATTCTTGTACACAGGCATTGACAATGCTTCTGATGCCAGTGGTGATCAGTCATGTTTACAATTCAGTAGGTGCTCAGGTGAAATAAGTCAGGTTGATTCGCCTAACTCAGTGAGCAATAAGAAGATTGGTTCTGTGAATAATGCTGCAAGGCGCCTTAAGTATCTTCTTCAATGGCCAAGGAGGAAAGAAAAAAAGGAATCTAGCAGAGACTTGGGAGATGCTGATTCCGTGGATTCATATAGTCCATCTACAGATTTGGAAGACAATCCAATCCCACTTCGGCAGATGTACTCAAAATCTTCATCCCTTCCCAACAACAAAAGGATACAGTCTGCTAGGACTTATCTTCCAAGCCCCTATACCAAAATGAAGTATACTGCTGGTCTAACACATGGTGTAATTCAAGCAGTGCCTAATTTTGCTTTTCCAGCTCAATCAAATGTAAGTCCTCTGTCACGATCATCAATGTCTTCACCTGCCTTGGTGGACAAGGAGAAGGGTGTTGACGATGCAGGATTGTCTCGTGCAAATGGGAAAGCATCAGTGGTGCACTCCAGACAAAGTTCCTACAATAGGAAGTTGATAAACCACTATTTGTGTGTTGGTGCACAAGGTGTGGAGGCTGAAGAATCAATTAGCTCTACATGGCCAACTCGGAGCTTCAAGCATTCCACTTCCTTAGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

662

Amino Acids

72.56

Weight (kDa)

8.27

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 188 - 273 7.1e-16 Ankyrin repeats (3 copies)
Ank_2 PF12796 247 - 292 6.4e-06 Ankyrin repeats (3 copies)
Ank PF00023 249 - 275 2e-06 Ankyrin repeat
Ank_2 PF12796 306 - 372 6.5e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 325 - 373 7.9e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1780
AatII GACGTC 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 1780
AccB1I GGYRCC 1 cut(s) 1446
AccBSI CCGCTC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 1670
AccII CGCG 1 cut(s) 59
AccIII TCCGGA 1 cut(s) 900
AclWI GGATC 1 cut(s) 280
AcoI YGGCCR 4 cut(s) 88, 271, 1469, 1943
AcuI CTGAAG 2 cut(s) 187, 1941
AcyI GRCGYC 3 cut(s) 149, 1106, 1447
AdeI CACNNNGTG 2 cut(s) 1691, 1910
AfaI GTAC 4 cut(s) 14, 56, 1304, 1586
AfeI AGCGCT 1 cut(s) 804
AfiI CCNNNNNNNGG 5 cut(s) 379, 491, 777, 907, 1869
AflII CTTAAG 2 cut(s) 1121, 1451
AflIII ACRYGT 2 cut(s) 57, 763
AgsI TTSAA 4 cut(s) 535, 1466, 1700, 1960
AjiI CACGTC 2 cut(s) 211, 340
AjnI CCWGG 2 cut(s) 892, 1261
AjuI GAANNNNNNNTTGG 2 cut(s) 792, 824
AleI CACNNNNGTG 1 cut(s) 204
Alw21I GWGCWC 5 cut(s) 892, 927, 1370, 1848, 1906
Alw26I GTCTC 4 cut(s) 36, 363, 1497, 1821
Alw44I GTGCAC 2 cut(s) 1844, 1902
AlwI GGATC 1 cut(s) 280
AlwNI CAGNNNCTG 1 cut(s) 878
Aor13HI TCCGGA 1 cut(s) 900
Aor51HI AGCGCT 1 cut(s) 804
AoxI GGCC 6 cut(s) 88, 271, 291, 1130, 1469, 1943
ApaLI GTGCAC 2 cut(s) 1844, 1902
ApeKI GCWGC 6 cut(s) 77, 80, 310, 425, 463, 1439
ArsI GACNNNNNNTTYG 4 cut(s) 329, 361, 1127, 1159
AseI ATTAAT 1 cut(s) 975
AspLEI GCGC 2 cut(s) 805, 1449
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 2 cut(s) 290, 554
AsuNHI GCTAGC 1 cut(s) 826
BaeGI GKGCMC 2 cut(s) 1848, 1906
BalI TGGCCA 3 cut(s) 273, 1471, 1945
BanI GGYRCC 1 cut(s) 1446
BanII GRGCYC 3 cut(s) 653, 892, 927
BauI CACGAG 2 cut(s) 251, 1818
BbsI GAAGAC 3 cut(s) 1137, 1563, 1758
Bbv12I GWGCWC 5 cut(s) 892, 927, 1370, 1848, 1906
BbvCI CCTCAGC 1 cut(s) 328
BbvI GCAGC 6 cut(s) 89, 92, 297, 412, 450, 1426
BccI CCATC 4 cut(s) 1075, 1085, 1091, 1549
BceAI ACGGC 2 cut(s) 103, 316
BcgI CGANNNNNNTGC 2 cut(s) 804, 838
BciT130I CCWGG 2 cut(s) 894, 1263
BciVI GTATCC 1 cut(s) 1614
BclI TGATCA 2 cut(s) 1158, 1339
BcnI CCSGG 2 cut(s) 290, 554
BcoDI GTCTC 4 cut(s) 36, 363, 1497, 1821
BfaI CTAG 8 cut(s) 432, 467, 587, 827, 1038, 1112, 1497, 1632
BfmI CTRYAG 2 cut(s) 735, 1545
BfoI RGCGCY 2 cut(s) 806, 1450
BfrI CTTAAG 2 cut(s) 1121, 1451
BfuAI ACCTGC 1 cut(s) 1780
BfuI GTATCC 1 cut(s) 1614
BglII AGATCT 1 cut(s) 1075
Bme1390I CCNGG 4 cut(s) 290, 554, 894, 1263
BmgBI CACGTC 2 cut(s) 211, 340
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 2 cut(s) 1267, 1448
BmrFI CCNGG 4 cut(s) 290, 554, 894, 1263
BmrI ACTGGG 1 cut(s) 82
BmtI GCTAGC 1 cut(s) 830
BmuI ACTGGG 1 cut(s) 82
BpiI GAAGAC 3 cut(s) 1137, 1563, 1758
BpmI CTGGAG 2 cut(s) 1200, 1834
Bpu10I CCTNAGC 3 cut(s) 328, 645, 1369
BpuEI CTTGAG 1 cut(s) 213
BpuMI CCSGG 2 cut(s) 290, 554
BsaHI GRCGYC 3 cut(s) 149, 1106, 1447
BsaI GGTCTC 2 cut(s) 36, 363
BsaJI CCNNGG 4 cut(s) 485, 1472, 1524, 1776
BsaWI WCCGGW 3 cut(s) 24, 38, 900
BsaXI ACNNNNNCTCC 6 cut(s) 383, 413, 509, 521, 539, 551
Bsc4I CCNNNNNNNGG 5 cut(s) 379, 491, 777, 907, 1869
Bse118I RCCGGY 2 cut(s) 293, 332
Bse1I ACTGG 2 cut(s) 77, 1332
Bse3DI GCAATG 1 cut(s) 508
BseAI TCCGGA 1 cut(s) 900
BseBI CCWGG 2 cut(s) 894, 1263
BseDI CCNNGG 4 cut(s) 485, 1472, 1524, 1776
BseGI GGATG 6 cut(s) 162, 620, 646, 713, 1083, 1598
BseLI CCNNNNNNNGG 5 cut(s) 379, 491, 777, 907, 1869
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 4 cut(s) 342, 659, 1383, 1416
BseNI ACTGG 2 cut(s) 77, 1332
BseRI GAGGAG 2 cut(s) 125, 1043
BseSI GKGCMC 2 cut(s) 1848, 1906
BseXI GCAGC 6 cut(s) 89, 92, 297, 412, 450, 1426
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 6 cut(s) 90, 273, 293, 1132, 1471, 1945
BshNI GGYRCC 1 cut(s) 1446
BsiHKAI GWGCWC 5 cut(s) 892, 927, 1370, 1848, 1906
BsiSI CCGG 7 cut(s) 25, 39, 289, 294, 333, 553, 901
BslFI GGGAC 2 cut(s) 136, 200
BslI CCNNNNNNNGG 5 cut(s) 379, 491, 777, 907, 1869
BsmAI GTCTC 4 cut(s) 36, 363, 1497, 1821
BsmFI GGGAC 2 cut(s) 136, 200
BsmI GAATGC 2 cut(s) 1245, 1963
BsnI GGCC 6 cut(s) 90, 273, 293, 1132, 1471, 1945
Bso31I GGTCTC 2 cut(s) 36, 363
Bsp1286I GDGCHC 6 cut(s) 653, 892, 927, 1370, 1848, 1906
Bsp13I TCCGGA 1 cut(s) 900
Bsp1407I TGTACA 1 cut(s) 1302
Bsp143I GATC 6 cut(s) 285, 496, 1075, 1158, 1339, 1754
BspANI GGCC 6 cut(s) 90, 273, 293, 1132, 1471, 1945
BspCNI CTCAG 4 cut(s) 341, 658, 1382, 1415
BspEI TCCGGA 1 cut(s) 900
BspFNI CGCG 1 cut(s) 59
BspHI TCATGA 1 cut(s) 1056
BspLI GGNNCC 2 cut(s) 1267, 1448
BspMI ACCTGC 1 cut(s) 1780
BspOI GCTAGC 1 cut(s) 830
BspPI GGATC 1 cut(s) 280
BspQI GCTCTTC 1 cut(s) 1011
BspT107I GGYRCC 1 cut(s) 1446
BspTI CTTAAG 2 cut(s) 1121, 1451
BspTNI GGTCTC 2 cut(s) 36, 363
BsrBI CCGCTC 1 cut(s) 225
BsrDI GCAATG 1 cut(s) 508
BsrFI RCCGGY 2 cut(s) 293, 332
BsrGI TGTACA 1 cut(s) 1302
BsrI ACTGG 2 cut(s) 77, 1332
BssAI RCCGGY 2 cut(s) 293, 332
BssECI CCNNGG 4 cut(s) 485, 1472, 1524, 1776
BssMI GATC 6 cut(s) 285, 496, 1075, 1158, 1339, 1754
BssNAI GTATAC 1 cut(s) 1671
BssNI GRCGYC 3 cut(s) 149, 1106, 1447
BssSI CACGAG 2 cut(s) 251, 1818
BssT1I CCWWGG 2 cut(s) 1472, 1776
Bst1107I GTATAC 1 cut(s) 1671
Bst2BI CACGAG 2 cut(s) 251, 1818
Bst2UI CCWGG 2 cut(s) 894, 1263
Bst4CI ACNGT 5 cut(s) 181, 658, 1063, 1143, 1626
Bst6I CTCTTC 1 cut(s) 1011
BstACI GRCGYC 3 cut(s) 149, 1106, 1447
BstAFI CTTAAG 2 cut(s) 1121, 1451
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstAUI TGTACA 1 cut(s) 1302
BstC8I GCNNGC 4 cut(s) 110, 295, 308, 828
BstDEI CTNAG 5 cut(s) 328, 645, 1369, 1402, 1975
BstDSI CCRYGG 1 cut(s) 1524
BstENI CCTNNNNNAGG 1 cut(s) 1867
BstF5I GGATG 6 cut(s) 162, 620, 646, 713, 1083, 1598
BstFNI CGCG 1 cut(s) 59
BstH2I RGCGCY 2 cut(s) 806, 1450
BstHHI GCGC 2 cut(s) 805, 1449
BstKTI GATC 6 cut(s) 288, 499, 1078, 1161, 1342, 1757
BstMAI GTCTC 4 cut(s) 36, 363, 1497, 1821
BstMBI GATC 6 cut(s) 285, 496, 1075, 1158, 1339, 1754
BstMWI GCNNNNNNNGC 9 cut(s) 165, 168, 303, 329, 563, 931, 1217, 1328, 1724
BstNI CCWGG 2 cut(s) 894, 1263
BstNSI RCATGY 2 cut(s) 767, 964
BstSCI CCNGG 4 cut(s) 288, 552, 892, 1261
BstSFI CTRYAG 2 cut(s) 735, 1545
BstSLI GKGCMC 2 cut(s) 1848, 1906
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 6 cut(s) 89, 92, 297, 412, 450, 1426
BstV2I GAAGAC 3 cut(s) 1137, 1563, 1758
BstX2I RGATCY 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 1075
BstZ17I GTATAC 1 cut(s) 1671
BsuI GTATCC 1 cut(s) 1614
BsuRI GGCC 6 cut(s) 90, 273, 293, 1132, 1471, 1945
BtgI CCRYGG 1 cut(s) 1524
BtrI CACGTC 2 cut(s) 211, 340
BtsCI GGATG 6 cut(s) 162, 620, 646, 713, 1083, 1598
BtsI GCAGTG 2 cut(s) 879, 1710
BtsIMutI CAGTG 6 cut(s) 211, 879, 1339, 1410, 1710, 1845
BveI ACCTGC 1 cut(s) 1780
Cac8I GCNNGC 4 cut(s) 110, 295, 308, 828
CaiI CAGNNNCTG 1 cut(s) 878
CciI TCATGA 1 cut(s) 1056
CfoI GCGC 2 cut(s) 805, 1449
Cfr10I RCCGGY 2 cut(s) 293, 332
Cfr13I GGNCC 1 cut(s) 291
CseI GACGC 3 cut(s) 48, 271, 1114
Csp6I GTAC 4 cut(s) 13, 55, 1303, 1585
CviAII CATG 9 cut(s) 563, 764, 961, 1057, 1102, 1244, 1347, 1688, 1941
CviQI GTAC 4 cut(s) 13, 55, 1303, 1585
DdeI CTNAG 5 cut(s) 328, 645, 1369, 1402, 1975
DinI GGCGCC 1 cut(s) 1448
DpnI GATC 6 cut(s) 287, 498, 1077, 1160, 1341, 1756
DpnII GATC 6 cut(s) 285, 496, 1075, 1158, 1339, 1754
DraIII CACNNNGTG 2 cut(s) 1691, 1910
EaeI YGGCCR 4 cut(s) 88, 271, 1469, 1943
Eam1104I CTCTTC 1 cut(s) 1011
EarI CTCTTC 1 cut(s) 1011
EciI GGCGGA 2 cut(s) 146, 155
Ecl136II GAGCTC 2 cut(s) 890, 925
Eco130I CCWWGG 2 cut(s) 1472, 1776
Eco24I GRGCYC 3 cut(s) 653, 892, 927
Eco31I GGTCTC 2 cut(s) 36, 363
Eco32I GATATC 2 cut(s) 415, 724
Eco47III AGCGCT 1 cut(s) 804
Eco53kI GAGCTC 2 cut(s) 890, 925
Eco57I CTGAAG 2 cut(s) 187, 1941
EcoICRI GAGCTC 2 cut(s) 890, 925
EcoNI CCTNNNNNAGG 1 cut(s) 1867
EcoRII CCWGG 2 cut(s) 892, 1261
EcoRV GATATC 2 cut(s) 415, 724
EcoT14I CCWWGG 2 cut(s) 1472, 1776
EcoT22I ATGCAT 1 cut(s) 1245
EcoT38I GRGCYC 3 cut(s) 653, 892, 927
EgeI GGCGCC 1 cut(s) 1448
EheI GGCGCC 1 cut(s) 1448
ErhI CCWWGG 2 cut(s) 1472, 1776
FaeI CATG 9 cut(s) 566, 767, 964, 1060, 1105, 1247, 1350, 1691, 1944
FaqI GGGAC 2 cut(s) 136, 200
FatI CATG 9 cut(s) 562, 763, 960, 1056, 1101, 1243, 1346, 1687, 1940
FauNDI CATATG 1 cut(s) 867
FbaI TGATCA 2 cut(s) 1158, 1339
FblI GTMKAC 1 cut(s) 1670
FokI GGATG 6 cut(s) 149, 627, 653, 700, 1070, 1585
FriOI GRGCYC 3 cut(s) 653, 892, 927
FspBI CTAG 8 cut(s) 432, 467, 587, 827, 1038, 1112, 1497, 1632
GlaI GCGC 2 cut(s) 804, 1448
GsuI CTGGAG 2 cut(s) 1200, 1834
HaeII RGCGCY 2 cut(s) 806, 1450
HaeIII GGCC 6 cut(s) 90, 273, 293, 1132, 1471, 1945
HapII CCGG 7 cut(s) 25, 39, 289, 294, 333, 553, 901
HgaI GACGC 3 cut(s) 48, 271, 1114
HhaI GCGC 2 cut(s) 805, 1449
Hin1I GRCGYC 3 cut(s) 149, 1106, 1447
Hin1II CATG 9 cut(s) 566, 767, 964, 1060, 1105, 1247, 1350, 1691, 1944
Hin6I GCGC 2 cut(s) 803, 1447
HinP1I GCGC 2 cut(s) 803, 1447
HincII GTYRAC 1 cut(s) 1801
HindII GTYRAC 1 cut(s) 1801
HinfI GANTC 6 cut(s) 1278, 1391, 1493, 1520, 1529, 1925
HpaII CCGG 7 cut(s) 25, 39, 289, 294, 333, 553, 901
Hpy166II GTNNAC 9 cut(s) 910, 958, 1305, 1352, 1671, 1783, 1801, 1846, 1904
Hpy188I TCNGA 9 cut(s) 285, 529, 708, 887, 1080, 1150, 1277, 1327, 1953
Hpy188III TCNNGA 6 cut(s) 587, 901, 1057, 1199, 1752, 1851
Hpy8I GTNNAC 9 cut(s) 910, 958, 1305, 1352, 1671, 1783, 1801, 1846, 1904
Hpy99I CGWCG 3 cut(s) 13, 64, 194
HpyAV CCTTC 2 cut(s) 1613, 1786
HpyCH4III ACNGT 5 cut(s) 181, 658, 1063, 1143, 1626
HpyCH4IV ACGT 3 cut(s) 149, 210, 339
HpyF10VI GCNNNNNNNGC 9 cut(s) 165, 168, 303, 329, 563, 931, 1217, 1328, 1724
HpyF3I CTNAG 5 cut(s) 328, 645, 1369, 1402, 1975
HpySE526I ACGT 3 cut(s) 149, 210, 339
Hsp92I GRCGYC 3 cut(s) 149, 1106, 1447
Hsp92II CATG 9 cut(s) 566, 767, 964, 1060, 1105, 1247, 1350, 1691, 1944
HspAI GCGC 2 cut(s) 803, 1447
KasI GGCGCC 1 cut(s) 1446
Kpn2I TCCGGA 1 cut(s) 900
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Ksp22I TGATCA 2 cut(s) 1158, 1339
Kzo9I GATC 6 cut(s) 285, 496, 1075, 1158, 1339, 1754
LguI GCTCTTC 1 cut(s) 1011
LmnI GCTCC 2 cut(s) 895, 1953
Lsp1109I GCAGC 6 cut(s) 89, 92, 297, 412, 450, 1426
MaeI CTAG 8 cut(s) 432, 467, 587, 827, 1038, 1112, 1497, 1632
MaeII ACGT 3 cut(s) 149, 210, 339
MaeIII GTNAC 6 cut(s) 206, 335, 406, 782, 1137, 1749
MalI GATC 6 cut(s) 287, 498, 1077, 1160, 1341, 1756
MbiI CCGCTC 1 cut(s) 225
MboI GATC 6 cut(s) 285, 496, 1075, 1158, 1339, 1754
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 6 cut(s) 653, 892, 927, 1370, 1848, 1906
MlsI TGGCCA 3 cut(s) 273, 1471, 1945
MluCI AATT 6 cut(s) 807, 935, 1355, 1695, 1711, 1929
MluI ACGCGT 1 cut(s) 57
MluNI TGGCCA 3 cut(s) 273, 1471, 1945
Mly113I GGCGCC 1 cut(s) 1447
MmeI TCCRAC 1 cut(s) 507
Mox20I TGGCCA 3 cut(s) 273, 1471, 1945
Mph1103I ATGCAT 1 cut(s) 1245
MroI TCCGGA 1 cut(s) 900
MroNI GCCGGC 1 cut(s) 293
MscI TGGCCA 3 cut(s) 273, 1471, 1945
MseI TTAA 5 cut(s) 351, 699, 975, 1122, 1452
MslI CAYNNNNRTG 1 cut(s) 204
Msp20I TGGCCA 3 cut(s) 273, 1471, 1945
MspA1I CMGCKG 1 cut(s) 1178
MspCI CTTAAG 2 cut(s) 1121, 1451
MspI CCGG 7 cut(s) 25, 39, 289, 294, 333, 553, 901
MspR9I CCNGG 4 cut(s) 290, 554, 894, 1263
Mva1269I GAATGC 2 cut(s) 1245, 1963
MvaI CCWGG 2 cut(s) 894, 1263
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 9 cut(s) 165, 168, 303, 329, 563, 931, 1217, 1328, 1724
NaeI GCCGGC 1 cut(s) 295
NarI GGCGCC 1 cut(s) 1447
NciI CCSGG 2 cut(s) 290, 554
NdeI CATATG 1 cut(s) 867
NdeII GATC 6 cut(s) 285, 496, 1075, 1158, 1339, 1754
NgoMIV GCCGGC 1 cut(s) 293
NheI GCTAGC 1 cut(s) 826
NlaIII CATG 9 cut(s) 566, 767, 964, 1060, 1105, 1247, 1350, 1691, 1944
NlaIV GGNNCC 2 cut(s) 1267, 1448
NmeAIII GCCGAG 1 cut(s) 510
NmuCI GTSAC 6 cut(s) 206, 335, 406, 782, 1137, 1749
NsiI ATGCAT 1 cut(s) 1245
NspI RCATGY 2 cut(s) 767, 964
OliI CACNNNNGTG 1 cut(s) 204
PagI TCATGA 1 cut(s) 1056
PaqCI CACCTGC 1 cut(s) 1780
PciI ACATGT 1 cut(s) 763
PciSI GCTCTTC 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 482
PctI GAATGC 2 cut(s) 1245, 1963
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 6 cut(s) 1278, 1391, 1493, 1520, 1529, 1925
PflFI GACNNNGTC 1 cut(s) 101
PfoI TCCNGGA 1 cut(s) 1261
PluTI GGCGCC 1 cut(s) 1450
PscI ACATGT 1 cut(s) 763
PshBI ATTAAT 1 cut(s) 975
Psp124BI GAGCTC 2 cut(s) 892, 927
Psp6I CCWGG 2 cut(s) 892, 1261
PspGI CCWGG 2 cut(s) 892, 1261
PspN4I GGNNCC 2 cut(s) 1267, 1448
PspPI GGNCC 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 1 cut(s) 1075
PsyI GACNNNGTC 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 1178
RsaI GTAC 4 cut(s) 14, 56, 1304, 1586
RsaNI GTAC 4 cut(s) 13, 55, 1303, 1585
RseI CAYNNNNRTG 1 cut(s) 204
SacI GAGCTC 2 cut(s) 892, 927
SapI GCTCTTC 1 cut(s) 1011
SaqAI TTAA 5 cut(s) 351, 699, 975, 1122, 1452
Sau3AI GATC 6 cut(s) 285, 496, 1075, 1158, 1339, 1754
Sau96I GGNCC 1 cut(s) 291
ScrFI CCNGG 4 cut(s) 290, 554, 894, 1263
SduI GDGCHC 6 cut(s) 653, 892, 927, 1370, 1848, 1906
SfcI CTRYAG 2 cut(s) 735, 1545
SfoI GGCGCC 1 cut(s) 1448
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 3 cut(s) 228, 1121, 1451
SmoI CTYRAG 3 cut(s) 228, 1121, 1451
Sse9I AATT 6 cut(s) 807, 935, 1355, 1695, 1711, 1929
SspDI GGCGCC 1 cut(s) 1446
SspMI CTAG 8 cut(s) 432, 467, 587, 827, 1038, 1112, 1497, 1632
SstI GAGCTC 2 cut(s) 892, 927
StyD4I CCNGG 4 cut(s) 288, 552, 892, 1261
StyI CCWWGG 2 cut(s) 1472, 1776
TaaI ACNGT 5 cut(s) 181, 658, 1063, 1143, 1626
TaiI ACGT 3 cut(s) 152, 213, 342
TaqI TCGA 3 cut(s) 123, 174, 839
TasI AATT 6 cut(s) 807, 935, 1355, 1695, 1711, 1929
TatI WGTACW 2 cut(s) 1302, 1584
TfiI GAWTC 6 cut(s) 1278, 1391, 1493, 1520, 1529, 1925
Tru1I TTAA 5 cut(s) 351, 699, 975, 1122, 1452
Tru9I TTAA 5 cut(s) 351, 699, 975, 1122, 1452
TscAI CASTG 6 cut(s) 211, 879, 1339, 1410, 1710, 1845
TseFI GTSAC 6 cut(s) 206, 335, 406, 782, 1137, 1749
TseI GCWGC 6 cut(s) 77, 80, 310, 425, 463, 1439
Tsp45I GTSAC 6 cut(s) 206, 335, 406, 782, 1137, 1749
TspDTI ATGAA 5 cut(s) 551, 563, 1521, 1587, 1679
TspGWI ACGGA 2 cut(s) 1076, 1513
TspRI CASTG 6 cut(s) 211, 879, 1339, 1410, 1710, 1845
Tth111I GACNNNGTC 1 cut(s) 101
Vha464I CTTAAG 2 cut(s) 1121, 1451
VneI GTGCAC 2 cut(s) 1844, 1902
VspI ATTAAT 1 cut(s) 975
XagI CCTNNNNNAGG 1 cut(s) 1867
XbaI TCTAGA 1 cut(s) 586
XceI RCATGY 2 cut(s) 767, 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 1670
XspI CTAG 8 cut(s) 432, 467, 587, 827, 1038, 1112, 1497, 1632
ZraI GACGTC 1 cut(s) 150
Zsp2I ATGCAT 1 cut(s) 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.