MD17G1017100.v1.1

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002201
Physical Location & Seq
Reverse (-)
1367024 .. 1370769
3746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1017100.v1.1.491

Sequence Viewer

Length: 2058 bp
ATGCCTCCCACATATTTTCCTCTGCGGTGGGAGAGCACTGGAGACCAATGGTGGTACGCGTCTCCGATCGACTACGCAGCCGCTAACGGCCACTACGACTTGGTTCGAGAGCTCCTTCGTATAGACGGCAACCACCTCATCAAGCTCACATCTCTCCGCCGCATCCGCCGTCTAGAGACAGTGTGGGACGACGAAGCACAGTATGATGATGTAGCAAAGTGTCGTTCGCGCGTTGCGCGGAAGCTCTTCCACGAATGCGAGTCCAAGAAAGAAAAAACAAACTCTCTCATAAGGGCTGGATACGGCGGTTGGCTTATGTACACCGCCGCCTCCGCCGGGGAGCTTGGTTTTGTCCAAGAACTTCTCGAGAGAAACCCTCTGCTTGTTTTCGGGGAAGGAGAATATGGGGTGACTGATATTTTCTATGCTGCTGCAAGGAGCAAGAATGTTGAGGTTTTCAGGCTGCTTTTTGACTTTGCGGTTTCGCCGAGGTTTATGACTGGGAGCCGCGGAGAGCTGGAGGAGCACGTTGGGGAGATTCCAAGTGTTTATAAACGGGAAATGCTTAACAGAGCGGTTCATGCAGCGGCGAGAGGCGGAAATTTGAGTATTTTGAAGGACCTTCTTTCGGATTGTGAGGATGTTTTGGCTTATAGGGATATTCAGGCATCAACTGTGCTCCATGCAGCGGCCGGGAAAGGACGGGTAGAGGTAGTGAAGTACCTTGTGGCATCCTTTGATATCATAAACTCCACTGACCGCCAGGGCAACACAGCATTGCATGTTGCAGCTTCAAGAGGCCAACTTGCCGCAGCCGAAGCGCTTATATCTGCATCCCCTTCATCCATTTCTGCACGAAACAACTCTGGAGAAACGTTTCTCCACAAGGCTATATCCGGCTTCCAAAGTCCTGCCTTCCGAAGACTGGACAGACAAATTCAGCTTCTGAAGCAACTGGTGAGCGAGAAAGCTTTCACTATCGAACACATCATCAATGCGAAAAACAATGAAGGACGGACTGCCCTTCACACGGCAATCATTGGGGATGTGCATTCCGATTTAGTCCAACTTCTGATGATTGCAAAATCAATTGATGTGAATGCCCGCGACATGGATGGCATGACAGCACTTGATTACCTTAGACAATGGCCTCGTTCAGCATCATCTGATATACTAATCAGGCAGCTGATTTCGGCTGGGGGAATATTTGGTTGTGAGGATTACAATGCAAGAAAAGCGATTGCTTCGCGCTTGAAGATGCAAGGCGATGGAGGTAGTCCCGGAACATCATTTAGAATTTCTGACACGGAAATCTTCTTGTATACTGGCATTGAAAATGTATCAGATGCTACTGCTGATCGCCGTAGCATAGGAACCCATTCACCTGCACCCGAACTTATCAGTCCGTATGATCCAACCACTGAGAATAACGGCAGCTCATTCTCTGGTAAAAAACCTGGTTCTGTAAATAATGCAGCAGCACAACTAAAACGTGTCATAGGCTGGCCACAAATGAAAGCGAAAAAGCACGAAAGGTTCAAGAAATCGGTTGATTTGGGATCGGTAGATTCTATCAAGATATGCAACAAAAGCCCAGATGATGCAGCCCCAACCCCACTTCGACAGAGATTTTCAAAGCCCTCAGTCTCATCAGTTCCCAGCAACAAACGGACACTTTCCGTGAGGAGCAACCAGTCAAGTCCATCAGCGAAAAAGAGGTTTGCTTGCGGCATAAGGCACGGCGTAATGCAGGCAATTCCGCACATAACTGTGCCAGGTCGTTCTCGGTCTAGTTCATTTTCGAAATCATCTTCAATTTCTTCACCTACTTCTCTGGATAAACAGAAAAGCGTTTGCATGGAAACCGACATTGCAGGACCATCTTGGTCGAATGAAGTAGTTGATGACGAAACACCGAATTTGGCAAAACAAGGCTCTGTTCGTAGAAGGTTGAGGAGCCATTATTTCTGTTTTGGTGCATCAGGATTGTCTGTGAAAACCCCAGTTCACAGGCAGCAGCAACGTTTCAAGCATCCTCCTGTTATTTCAGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

686

Amino Acids

75.49

Weight (kDa)

9.44

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 194 - 278 1.6e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 227 - 276 1.8e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 249 - 297 6.4e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 250 - 300 3.5e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 331 - 379 5.1e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AarI CACCTGC 1 cut(s) 1393
Acc36I ACCTGC 1 cut(s) 1393
AccBSI CCGCTC 1 cut(s) 575
AccI GTMKAC 1 cut(s) 1322
AccII CGCG 7 cut(s) 59, 229, 231, 238, 510, 1107, 1249
AclI AACGTT 2 cut(s) 875, 2023
AclWI GGATC 2 cut(s) 1406, 1567
AcoI YGGCCR 3 cut(s) 88, 690, 1505
AcsI RAATTY 4 cut(s) 601, 936, 1296, 1918
AcuI CTGAAG 1 cut(s) 968
AfaI GTAC 3 cut(s) 56, 320, 722
AfeI AGCGCT 1 cut(s) 822
AfiI CCNNNNNNNGG 6 cut(s) 336, 628, 688, 925, 1030, 1111
AflIII ACRYGT 2 cut(s) 57, 1492
AgsI TTSAA 8 cut(s) 616, 795, 1255, 1334, 1540, 1635, 1815, 2029
AjnI CCWGG 3 cut(s) 762, 1456, 1774
Alw21I GWGCWC 4 cut(s) 38, 114, 528, 681
Alw26I GTCTC 4 cut(s) 36, 66, 170, 1651
AlwI GGATC 2 cut(s) 1406, 1567
AlwNI CAGNNNCTG 1 cut(s) 946
Ama87I CYCGRG 1 cut(s) 365
Aor51HI AGCGCT 1 cut(s) 822
AoxI GGCC 5 cut(s) 88, 690, 799, 1148, 1505
ApoI RAATTY 4 cut(s) 601, 936, 1296, 1918
Asp700I GAANNNNTTC 4 cut(s) 245, 876, 971, 1378
AspLEI GCGC 4 cut(s) 231, 238, 823, 1251
AspS9I GGNCC 2 cut(s) 619, 1877
AsuC2I CCSGG 3 cut(s) 337, 694, 1281
AsuHPI GGTGA 4 cut(s) 421, 970, 1374, 1815
AsuII TTCGAA 1 cut(s) 1802
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 2 cut(s) 619, 1877
BaeI ACNNNNGTAYC 2 cut(s) 292, 325
BalI TGGCCA 1 cut(s) 1507
BanII GRGCYC 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 928
Bbv12I GWGCWC 4 cut(s) 38, 114, 528, 681
BccI CCATC 4 cut(s) 1109, 1262, 1711, 1888
BceAI ACGGC 8 cut(s) 103, 142, 153, 319, 1047, 1347, 1447, 1756
BciT130I CCWGG 3 cut(s) 764, 1458, 1776
BciVI GTATCC 1 cut(s) 293
BcnI CCSGG 3 cut(s) 337, 694, 1281
BcoDI GTCTC 4 cut(s) 36, 66, 170, 1651
BfaI CTAG 2 cut(s) 173, 1791
BfoI RGCGCY 1 cut(s) 824
BfuAI ACCTGC 1 cut(s) 1393
BfuI GTATCC 1 cut(s) 293
Bme1390I CCNGG 6 cut(s) 337, 694, 764, 1281, 1458, 1776
Bme18I GGWCC 2 cut(s) 619, 1877
BmeT110I CYCGRG 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 619, 1877
BmiI GGNNCC 3 cut(s) 506, 1375, 1958
BmrFI CCNGG 6 cut(s) 337, 694, 764, 1281, 1458, 1776
BmrI ACTGGG 2 cut(s) 510, 1997
BmuI ACTGGG 2 cut(s) 510, 1997
BpiI GAAGAC 1 cut(s) 928
BplI GAGNNNNNCTC 2 cut(s) 361, 393
BpmI CTGGAG 3 cut(s) 60, 539, 888
Bpu14I TTCGAA 1 cut(s) 1802
BpuMI CCSGG 3 cut(s) 337, 694, 1281
BsaI GGTCTC 1 cut(s) 36
BsaJI CCNNGG 4 cut(s) 336, 488, 508, 763
Bsc4I CCNNNNNNNGG 6 cut(s) 336, 628, 688, 925, 1030, 1111
Bse1I ACTGG 7 cut(s) 43, 505, 930, 960, 1330, 1693, 2003
Bse3DI GCAATG 2 cut(s) 776, 1869
BseBI CCWGG 3 cut(s) 764, 1458, 1776
BseDI CCNNGG 4 cut(s) 336, 488, 508, 763
BseGI GGATG 8 cut(s) 162, 646, 731, 833, 842, 1051, 1120, 2032
BseLI CCNNNNNNNGG 6 cut(s) 336, 628, 688, 925, 1030, 1111
BseMI GCAATG 2 cut(s) 776, 1869
BseMII CTCAG 2 cut(s) 1413, 1656
BseNI ACTGG 7 cut(s) 43, 505, 930, 960, 1330, 1693, 2003
BseRI GAGGAG 3 cut(s) 536, 1699, 1969
BseX3I CGGCCG 1 cut(s) 690
BseYI CCCAGC 2 cut(s) 1196, 1658
BsgI GTGCAG 2 cut(s) 837, 1371
Bsh1236I CGCG 7 cut(s) 59, 229, 231, 238, 510, 1107, 1249
Bsh1285I CGRYCG 2 cut(s) 69, 693
BshFI GGCC 5 cut(s) 90, 692, 801, 1150, 1507
BsiEI CGRYCG 2 cut(s) 69, 693
BsiHKAI GWGCWC 4 cut(s) 38, 114, 528, 681
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 4 cut(s) 336, 693, 897, 1281
BslFI GGGAC 2 cut(s) 200, 1263
BslI CCNNNNNNNGG 6 cut(s) 336, 628, 688, 925, 1030, 1111
BsmAI GTCTC 4 cut(s) 36, 66, 170, 1651
BsmBI CGTCTC 1 cut(s) 66
BsmFI GGGAC 2 cut(s) 200, 1263
BsmI GAATGC 3 cut(s) 260, 1051, 1105
BsnI GGCC 5 cut(s) 90, 692, 801, 1150, 1507
Bso31I GGTCTC 1 cut(s) 36
BsoBI CYCGRG 1 cut(s) 365
Bsp119I TTCGAA 1 cut(s) 1802
Bsp1286I GDGCHC 4 cut(s) 38, 114, 528, 681
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 4 cut(s) 66, 1357, 1411, 1559
BspANI GGCC 5 cut(s) 90, 692, 801, 1150, 1507
BspCNI CTCAG 2 cut(s) 1414, 1655
BspFNI CGCG 7 cut(s) 59, 229, 231, 238, 510, 1107, 1249
BspLI GGNNCC 3 cut(s) 506, 1375, 1958
BspMI ACCTGC 1 cut(s) 1393
BspPI GGATC 2 cut(s) 1406, 1567
BspQI GCTCTTC 1 cut(s) 251
BspT104I TTCGAA 1 cut(s) 1802
BspTNI GGTCTC 1 cut(s) 36
BsrBI CCGCTC 1 cut(s) 575
BsrDI GCAATG 2 cut(s) 776, 1869
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 7 cut(s) 43, 505, 930, 960, 1330, 1693, 2003
BssECI CCNNGG 4 cut(s) 336, 488, 508, 763
BssMI GATC 4 cut(s) 66, 1357, 1411, 1559
BssNAI GTATAC 1 cut(s) 1323
Bst1107I GTATAC 1 cut(s) 1323
Bst2UI CCWGG 3 cut(s) 764, 1458, 1776
Bst4CI ACNGT 4 cut(s) 181, 201, 676, 1771
Bst6I CTCTTC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 318
BstBI TTCGAA 1 cut(s) 1802
BstC8I GCNNGC 4 cut(s) 1105, 1505, 1726, 1752
BstDEI CTNAG 3 cut(s) 1139, 1422, 1642
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 8 cut(s) 162, 646, 731, 833, 842, 1051, 1120, 2032
BstFNI CGCG 7 cut(s) 59, 229, 231, 238, 510, 1107, 1249
BstH2I RGCGCY 1 cut(s) 824
BstHHI GCGC 4 cut(s) 231, 238, 823, 1251
BstKTI GATC 4 cut(s) 69, 1360, 1414, 1562
BstMAI GTCTC 4 cut(s) 36, 66, 170, 1651
BstMBI GATC 4 cut(s) 66, 1357, 1411, 1559
BstMCI CGRYCG 2 cut(s) 69, 693
BstMWI GCNNNNNNNGC 9 cut(s) 165, 235, 332, 523, 581, 818, 949, 1235, 1590
BstNI CCWGG 3 cut(s) 764, 1458, 1776
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 6 cut(s) 335, 692, 762, 1279, 1456, 1774
BstUI CGCG 7 cut(s) 59, 229, 231, 238, 510, 1107, 1249
BstV2I GAAGAC 1 cut(s) 928
BstZ17I GTATAC 1 cut(s) 1323
BstZI CGGCCG 1 cut(s) 690
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 5 cut(s) 90, 692, 801, 1150, 1507
BtgI CCRYGG 1 cut(s) 508
BtgZI GCGATG 1 cut(s) 1281
BtsCI GGATG 8 cut(s) 162, 646, 731, 833, 842, 1051, 1120, 2032
BtsIMutI CAGTG 4 cut(s) 36, 186, 753, 1419
BveI ACCTGC 1 cut(s) 1393
Cac8I GCNNGC 4 cut(s) 1105, 1505, 1726, 1752
CaiI CAGNNNCTG 1 cut(s) 946
CfoI GCGC 4 cut(s) 231, 238, 823, 1251
Cfr13I GGNCC 2 cut(s) 619, 1877
Cfr42I CCGCGG 1 cut(s) 511
CseI GACGC 1 cut(s) 48
CsiI ACCWGGT 1 cut(s) 1456
Csp6I GTAC 3 cut(s) 55, 319, 721
CviAII CATG 6 cut(s) 581, 683, 782, 1111, 1120, 1858
CviQI GTAC 3 cut(s) 55, 319, 721
DdeI CTNAG 3 cut(s) 1139, 1422, 1642
DpnI GATC 4 cut(s) 68, 1359, 1413, 1561
DpnII GATC 4 cut(s) 66, 1357, 1411, 1559
EaeI YGGCCR 3 cut(s) 88, 690, 1505
EagI CGGCCG 1 cut(s) 690
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 4 cut(s) 146, 155, 322, 612
Ecl136II GAGCTC 1 cut(s) 112
EclXI CGGCCG 1 cut(s) 690
Eco24I GRGCYC 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 36
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 2 cut(s) 619, 1877
Eco47III AGCGCT 1 cut(s) 822
Eco52I CGGCCG 1 cut(s) 690
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 968
Eco88I CYCGRG 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 112
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 3 cut(s) 762, 1456, 1774
EcoRV GATATC 1 cut(s) 742
EcoT38I GRGCYC 1 cut(s) 114
Esp3I CGTCTC 1 cut(s) 66
FaeI CATG 6 cut(s) 584, 686, 785, 1114, 1123, 1861
FaqI GGGAC 2 cut(s) 200, 1263
FatI CATG 6 cut(s) 580, 682, 781, 1110, 1119, 1857
FauI CCCGC 1 cut(s) 1112
FblI GTMKAC 1 cut(s) 1322
FokI GGATG 8 cut(s) 149, 653, 718, 820, 829, 1058, 1127, 2019
FriOI GRGCYC 1 cut(s) 114
FspBI CTAG 2 cut(s) 173, 1791
GlaI GCGC 4 cut(s) 230, 237, 822, 1250
GsaI CCCAGC 2 cut(s) 1200, 1662
GsuI CTGGAG 3 cut(s) 60, 539, 888
HaeII RGCGCY 1 cut(s) 824
HaeIII GGCC 5 cut(s) 90, 692, 801, 1150, 1507
HapII CCGG 4 cut(s) 336, 693, 897, 1281
HgaI GACGC 1 cut(s) 48
HhaI GCGC 4 cut(s) 231, 238, 823, 1251
Hin1II CATG 6 cut(s) 584, 686, 785, 1114, 1123, 1861
Hin6I GCGC 4 cut(s) 229, 236, 821, 1249
HinP1I GCGC 4 cut(s) 229, 236, 821, 1249
HindIII AAGCTT 1 cut(s) 969
HinfI GANTC 3 cut(s) 260, 538, 1568
HpaII CCGG 4 cut(s) 336, 693, 897, 1281
HphI GGTGA 4 cut(s) 421, 970, 1374, 1815
Hpy166II GTNNAC 3 cut(s) 321, 1323, 2008
Hpy188I TCNGA 9 cut(s) 66, 631, 920, 948, 1057, 1074, 1168, 1303, 1345
Hpy8I GTNNAC 3 cut(s) 321, 1323, 2008
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 9 cut(s) 125, 389, 610, 632, 849, 925, 1004, 1034, 1941
HpyCH4III ACNGT 4 cut(s) 181, 201, 676, 1771
HpyCH4IV ACGT 4 cut(s) 528, 875, 1492, 2023
HpyF10VI GCNNNNNNNGC 9 cut(s) 165, 235, 332, 523, 581, 818, 949, 1235, 1590
HpyF3I CTNAG 3 cut(s) 1139, 1422, 1642
HpySE526I ACGT 4 cut(s) 528, 875, 1492, 2023
Hsp92II CATG 6 cut(s) 584, 686, 785, 1114, 1123, 1861
HspAI GCGC 4 cut(s) 229, 236, 821, 1249
KspI CCGCGG 1 cut(s) 511
Kzo9I GATC 4 cut(s) 66, 1357, 1411, 1559
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 8 cut(s) 117, 340, 438, 504, 523, 684, 1686, 1956
MabI ACCWGGT 1 cut(s) 1456
MaeI CTAG 2 cut(s) 173, 1791
MaeII ACGT 4 cut(s) 528, 875, 1492, 2023
MaeIII GTNAC 1 cut(s) 409
MalI GATC 4 cut(s) 68, 1359, 1413, 1561
MbiI CCGCTC 1 cut(s) 575
MboI GATC 4 cut(s) 66, 1357, 1411, 1559
MboII GAAGA 6 cut(s) 238, 933, 1267, 1306, 1803, 1812
MfeI CAATTG 1 cut(s) 1089
MhlI GDGCHC 4 cut(s) 38, 114, 528, 681
MlsI TGGCCA 1 cut(s) 1507
MluCI AATT 7 cut(s) 601, 936, 1089, 1296, 1755, 1815, 1918
MluI ACGCGT 1 cut(s) 57
MluNI TGGCCA 1 cut(s) 1507
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 1090, 1439
Mox20I TGGCCA 1 cut(s) 1507
MroXI GAANNNNTTC 4 cut(s) 245, 876, 971, 1378
MscI TGGCCA 1 cut(s) 1507
MseI TTAA 1 cut(s) 567
MslI CAYNNNNRTG 1 cut(s) 1769
Msp20I TGGCCA 1 cut(s) 1507
MspA1I CMGCKG 4 cut(s) 510, 587, 689, 1186
MspI CCGG 4 cut(s) 336, 693, 897, 1281
MspR9I CCNGG 6 cut(s) 337, 694, 764, 1281, 1458, 1776
MunI CAATTG 1 cut(s) 1089
Mva1269I GAATGC 3 cut(s) 260, 1051, 1105
MvaI CCWGG 3 cut(s) 764, 1458, 1776
MvnI CGCG 7 cut(s) 59, 229, 231, 238, 510, 1107, 1249
MwoI GCNNNNNNNGC 9 cut(s) 165, 235, 332, 523, 581, 818, 949, 1235, 1590
NciI CCSGG 3 cut(s) 337, 694, 1281
NdeII GATC 4 cut(s) 66, 1357, 1411, 1559
NlaIII CATG 6 cut(s) 584, 686, 785, 1114, 1123, 1861
NlaIV GGNNCC 3 cut(s) 506, 1375, 1958
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 1 cut(s) 785
NspV TTCGAA 1 cut(s) 1802
PaeR7I CTCGAG 1 cut(s) 365
PaqCI CACCTGC 1 cut(s) 1393
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 3 cut(s) 260, 1051, 1105
PdmI GAANNNNTTC 4 cut(s) 245, 876, 971, 1378
PfeI GAWTC 2 cut(s) 538, 1568
PfoI TCCNGGA 1 cut(s) 1279
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 619
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 114
Psp1406I AACGTT 2 cut(s) 875, 2023
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 3 cut(s) 762, 1456, 1774
PspFI CCCAGC 2 cut(s) 1196, 1658
PspGI CCWGG 3 cut(s) 762, 1456, 1774
PspN4I GGNNCC 3 cut(s) 506, 1375, 1958
PspPI GGNCC 2 cut(s) 619, 1877
PspPPI RGGWCCY 1 cut(s) 619
PstNI CAGNNNCTG 1 cut(s) 946
PvuI CGATCG 1 cut(s) 69
PvuII CAGCTG 1 cut(s) 1186
RsaI GTAC 3 cut(s) 56, 320, 722
RsaNI GTAC 3 cut(s) 55, 319, 721
RseI CAYNNNNRTG 1 cut(s) 1769
SacI GAGCTC 1 cut(s) 114
SacII CCGCGG 1 cut(s) 511
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 567
Sau3AI GATC 4 cut(s) 66, 1357, 1411, 1559
Sau96I GGNCC 2 cut(s) 619, 1877
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 6 cut(s) 337, 694, 764, 1281, 1458, 1776
SduI GDGCHC 4 cut(s) 38, 114, 528, 681
SexAI ACCWGGT 1 cut(s) 1456
Sfr274I CTCGAG 1 cut(s) 365
Sfr303I CCGCGG 1 cut(s) 511
SfuI TTCGAA 1 cut(s) 1802
SgrBI CCGCGG 1 cut(s) 511
SinI GGWCC 2 cut(s) 619, 1877
SlaI CTCGAG 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 1769
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 7 cut(s) 601, 936, 1089, 1296, 1755, 1815, 1918
SspI AATATT 1 cut(s) 1206
SspMI CTAG 2 cut(s) 173, 1791
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 6 cut(s) 335, 692, 762, 1279, 1456, 1774
TaaI ACNGT 4 cut(s) 181, 201, 676, 1771
TaiI ACGT 4 cut(s) 531, 878, 1495, 2026
TaqI TCGA 7 cut(s) 69, 106, 366, 981, 1621, 1802, 1889
TaqII GACCGA 1 cut(s) 1776
TasI AATT 7 cut(s) 601, 936, 1089, 1296, 1755, 1815, 1918
TatI WGTACW 1 cut(s) 318
TauI GCSGC 8 cut(s) 83, 162, 329, 510, 590, 692, 812, 1731
TfiI GAWTC 2 cut(s) 538, 1568
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 4 cut(s) 43, 186, 760, 1426
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 6 cut(s) 569, 831, 1023, 1529, 1785, 1908
TspGWI ACGGA 5 cut(s) 1030, 1322, 1395, 1669, 1684
TspRI CASTG 4 cut(s) 43, 186, 760, 1426
VpaK11BI GGWCC 2 cut(s) 619, 1877
XapI RAATTY 4 cut(s) 601, 936, 1296, 1918
XbaI TCTAGA 1 cut(s) 172
XceI RCATGY 1 cut(s) 785
XhoI CTCGAG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 1322
XmnI GAANNNNTTC 4 cut(s) 245, 876, 971, 1378
XspI CTAG 2 cut(s) 173, 1791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.