MD13G1111600.v1.1

Ankyrin repeats (3 copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002201
Physical Location & Seq
Forward (+)
8123824 .. 8127015
3192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1111600.v1.1.491

Sequence Viewer

Length: 1956 bp
ATGCCACCATCTTACTTCCCTTTAAGGTGGGAAAGCACCGGAGACCAATGGTGGTATGCATCACCAATCGATTGGGCAGCAGCCAATGGCTTATATGATTTGGTCAGTGAGCTTCTTCACCTTGACACCAACCTCCTCATCAAGCTGACCTCATTGCGCCGCATCCGCCGCCTTGAAACTGTCTGGGACGACGAGGCTCAGTTCGATGATGTTGCCAAATGCCGGTCACAAGTTGCCAAGAGGCTCCTCCACGAGTGCCAAATGCAGAGGGGACACAACTCTCTCATCAGGGCCGGCTACGGCGGATGGCTTCTCTACACTGCGGCCTCGGCCGGGGATGTAGGGTTTGTCGGGGAGTTGCTGGAGAGAGACCCTCTTCTTGTTTTTGGAGAAGGAGAGTATGGAGTCACTGACATCTTCTATGCTGCTGCCAGGAGCAAGAACTCTGAGGTTTTCAGGCTGCTGCTTGATTTCTCAGTGTCTCCAAGGTTTAGCCTCAGTAATGGAGAATTCGAAGAGAGGTTGGGTGATGTTACATCGGATTTCAAGTGGGAAATGATGAACCGGGCGGTTCATGCCGCTGCTAGAGGTGGCAATTTGGAGATTTTGAGGGACCTGCTTGGGGATTGTGAGGATGTTTTGGCTTATAGAGATGCTCAGGGATCTACCATCTTGCATACAGCGTCTGGCAGAGGGCAGGTTGAGGTGGTTGAGTATCTAATAGCATTCTTCGATACCATCACAATAGTAGATAATCAAGGGAACACAGCTTTACACGTGGCCGCTTATAGGGGTCACCTGGCTGTGGTGGATGTCCTAATTCGTGCATCTCCCTCGTTAACCATGTCATCAAACAACTATGGAGACACCTTTCTGCATTTGGCAGTGGCTGGTTTCAGAAGCCCTGGTTTCCGGAGACTGGACAAACAGATTGAGCTCCTTAAGCAATTAGTATGTGGTGATATTGCGAACATGCAGGACGTTGTTAATGTTAAGAACAATGATGGACGAACTGCTCTTCACATGGCTGTAATTGAGAATGTTCAATCTAATTTGGTGGAACTCCTCATGACTCTTCCATCAATTGATTTGAACATCCGTGACTCTGGCGGCATGACCCCTCTAGATATTCTTAAACAAAGGCCGAAGTCAGCATCGTCCGAACTTCTGATCAAGGAATTGATTTCAGCTGGAGGGATCTCCAATCGTCAGGACCGTAAAGCAAGGAGCGCCCTTGTTTCCCATTTGAGAATGCGGGGTATTGGAGGCAGTCTGGGAACCTCTTTCAGAATTCCTGATGCAGAGATATTATTATACACGGGCATTGACAATGCTTCTGATGCCAATTGCGATCAGTCAGGTGTGCAATTCAACACGTTCTCAGGTGAGCTAAGACAGTTTGGCTCACCCAACACAGTAAACAATAAGAAGTCAAGTTCTGTAACTTATGCTGCAAGGCGCCTTAAGTTTCTTCTTCAATGGCGGAGGAGGAAAGAAAAAAAGGAATCTAGCATAGACTTAAGAGATAATGATTCTGTGGAGTCATTTAGCACCTGTGTAGATTTGGACGACAATCCAATCCCTCTTCGGCAGATGTATGCCAAATCTTCCTCCCTCCCAAACAACAAAAGGACAGTTTCCGTGAGGAGTTTTCTTCCGAGTCCATACACTAAAATGAAATTTACTGCTGGTTTATCACACGGTGTGATTCAGGCAAGATCATCTATGTCTTCCCCTAAGTTCATGGACAAAGAGAAGGGTGTTGACATTTTGGGACCCTCTAGTTCAAATGGCAAACCACCACATCCACAAGCGAGCTTCAAACATAATTCCCTCAGTAAGAAGTTAATAAACCAATATTTGTCTGTTGGCGCACAAGCCCTGGATGTGAATGAATCAATTAGCTCCACTTGGTCGAATCAAAATCACAAACACTCCGGTCCTCTAGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

652

Amino Acids

72.14

Weight (kDa)

7.68

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_4 PF13637 191 - 241 5e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 192 - 275 3.4e-15 Ankyrin repeats (3 copies)
Ank_2 PF12796 249 - 296 7.4e-07 Ankyrin repeats (3 copies)
Ank_5 PF13857 251 - 295 2.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 310 - 377 9.5e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 329 - 377 7.9e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 624, 688
AccB1I GGYRCC 1 cut(s) 1458
AccIII TCCGGA 1 cut(s) 912
AclWI GGATC 2 cut(s) 670, 1205
AcoI YGGCCR 2 cut(s) 330, 780
AcsI RAATTY 3 cut(s) 509, 1290, 1679
AcvI CACGTG 1 cut(s) 778
AcyI GRCGYC 1 cut(s) 1459
AdeI CACNNNGTG 2 cut(s) 1703, 1705
AfiI CCNNNNNNNGG 6 cut(s) 222, 333, 622, 789, 805, 919
AflII CTTAAG 3 cut(s) 941, 1463, 1519
AflIII ACRYGT 2 cut(s) 775, 1374
AgsI TTSAA 8 cut(s) 176, 547, 1046, 1093, 1372, 1478, 1788, 1822
AjnI CCWGG 4 cut(s) 431, 798, 904, 1881
AluBI AGCT 8 cut(s) 112, 145, 770, 937, 1190, 1390, 1818, 1905
AluI AGCT 8 cut(s) 112, 145, 770, 937, 1190, 1390, 1818, 1905
Alw21I GWGCWC 1 cut(s) 939
Alw26I GTCTC 5 cut(s) 36, 363, 486, 858, 910
AlwI GGATC 2 cut(s) 670, 1205
AlwNI CAGNNNCTG 2 cut(s) 686, 890
Aor13HI TCCGGA 1 cut(s) 912
AoxI GGCC 5 cut(s) 291, 324, 330, 780, 1142
ApeKI GCWGC 8 cut(s) 77, 80, 425, 428, 460, 463, 581, 1451
ApoI RAATTY 3 cut(s) 509, 1290, 1679
AspLEI GCGC 4 cut(s) 159, 1232, 1461, 1874
AspS9I GGNCC 5 cut(s) 291, 613, 1213, 1775, 1940
AsuC2I CCSGG 2 cut(s) 334, 566
AsuHPI GGTGA 7 cut(s) 54, 110, 539, 788, 971, 1397, 1398
AsuII TTCGAA 1 cut(s) 513
AvaII GGWCC 4 cut(s) 613, 1213, 1775, 1940
BanI GGYRCC 1 cut(s) 1458
BanII GRGCYC 1 cut(s) 939
BauI CACGAG 1 cut(s) 251
BbrPI CACGTG 1 cut(s) 778
BbsI GAAGAC 1 cut(s) 1722
Bbv12I GWGCWC 1 cut(s) 939
BbvI GCAGC 8 cut(s) 89, 92, 412, 415, 447, 450, 568, 1438
BccI CCATC 6 cut(s) 16, 300, 677, 746, 998, 1087
BceAI ACGGC 1 cut(s) 316
BcgI CGANNNNNNTGC 4 cut(s) 194, 228, 816, 850
BciT130I CCWGG 4 cut(s) 433, 800, 906, 1883
BclI TGATCA 1 cut(s) 1170
BcnI CCSGG 2 cut(s) 334, 566
BcoDI GTCTC 5 cut(s) 36, 363, 486, 858, 910
BfaI CTAG 5 cut(s) 585, 1124, 1509, 1782, 1946
BfoI RGCGCY 2 cut(s) 1233, 1462
BfrI CTTAAG 3 cut(s) 941, 1463, 1519
BfuAI ACCTGC 2 cut(s) 624, 688
Bme1390I CCNGG 6 cut(s) 334, 433, 566, 800, 906, 1883
Bme18I GGWCC 4 cut(s) 613, 1213, 1775, 1940
BmgT120I GGNCC 5 cut(s) 291, 613, 1213, 1775, 1940
BmiI GGNNCC 6 cut(s) 245, 614, 1279, 1460, 1776, 1777
BmrFI CCNGG 6 cut(s) 334, 433, 566, 800, 906, 1883
BmsI GCATC 7 cut(s) 68, 171, 643, 836, 1163, 1288, 1330
BpiI GAAGAC 1 cut(s) 1722
BplI GAGNNNNNCTC 2 cut(s) 358, 390
BpmI CTGGAG 2 cut(s) 383, 1212
Bpu10I CCTNAGC 1 cut(s) 657
Bpu14I TTCGAA 1 cut(s) 513
BpuMI CCSGG 2 cut(s) 334, 566
Bsa29I ATCGAT 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 778
BsaHI GRCGYC 1 cut(s) 1459
BsaI GGTCTC 2 cut(s) 36, 363
BsaJI CCNNGG 5 cut(s) 327, 333, 485, 904, 1881
BsaWI WCCGGW 3 cut(s) 38, 912, 1937
BsaXI ACNNNNNCTCC 2 cut(s) 1919, 1949
Bsc4I CCNNNNNNNGG 6 cut(s) 222, 333, 622, 789, 805, 919
Bse118I RCCGGY 2 cut(s) 222, 293
Bse1I ACTGG 1 cut(s) 924
Bse3DI GCAATG 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 912
BseBI CCWGG 4 cut(s) 433, 800, 906, 1883
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 5 cut(s) 327, 333, 485, 904, 1881
BseGI GGATG 8 cut(s) 162, 311, 343, 640, 817, 1095, 1804, 1891
BseLI CCNNNNNNNGG 6 cut(s) 222, 333, 622, 789, 805, 919
BseMI GCAATG 1 cut(s) 152
BseMII CTCAG 7 cut(s) 212, 438, 489, 511, 671, 1395, 1849
BseNI ACTGG 1 cut(s) 924
BseRI GAGGAG 5 cut(s) 125, 236, 1055, 1501, 1660
BseX3I CGGCCG 1 cut(s) 330
BseXI GCAGC 8 cut(s) 89, 92, 412, 415, 447, 450, 568, 1438
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 5 cut(s) 293, 326, 332, 782, 1144
BshNI GGYRCC 1 cut(s) 1458
BshVI ATCGAT 1 cut(s) 69
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 939
BsiSI CCGG 7 cut(s) 39, 223, 294, 333, 565, 913, 1938
BslFI GGGAC 4 cut(s) 200, 285, 626, 1788
BslI CCNNNNNNNGG 6 cut(s) 222, 333, 622, 789, 805, 919
BsmAI GTCTC 5 cut(s) 36, 363, 486, 858, 910
BsmFI GGGAC 4 cut(s) 200, 285, 626, 1788
BsmI GAATGC 2 cut(s) 725, 1257
BsnI GGCC 5 cut(s) 293, 326, 332, 782, 1144
Bso31I GGTCTC 2 cut(s) 36, 363
Bsp119I TTCGAA 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 939
Bsp13I TCCGGA 1 cut(s) 912
Bsp143I GATC 5 cut(s) 662, 1170, 1197, 1351, 1718
BspANI GGCC 5 cut(s) 293, 326, 332, 782, 1144
BspCNI CTCAG 7 cut(s) 211, 439, 488, 510, 670, 1394, 1848
BspDI ATCGAT 1 cut(s) 69
BspEI TCCGGA 1 cut(s) 912
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 6 cut(s) 245, 614, 1279, 1460, 1776, 1777
BspMI ACCTGC 2 cut(s) 624, 688
BspPI GGATC 2 cut(s) 670, 1205
BspQI GCTCTTC 1 cut(s) 1023
BspT104I TTCGAA 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 1458
BspTI CTTAAG 3 cut(s) 941, 1463, 1519
BspTNI GGTCTC 2 cut(s) 36, 363
BsrDI GCAATG 1 cut(s) 152
BsrFI RCCGGY 2 cut(s) 222, 293
BsrI ACTGG 1 cut(s) 924
BssAI RCCGGY 2 cut(s) 222, 293
BssECI CCNNGG 5 cut(s) 327, 333, 485, 904, 1881
BssMI GATC 5 cut(s) 662, 1170, 1197, 1351, 1718
BssNI GRCGYC 1 cut(s) 1459
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 485
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 4 cut(s) 433, 800, 906, 1883
Bst4CI ACNGT 6 cut(s) 181, 1217, 1398, 1417, 1636, 1703
Bst6I CTCTTC 5 cut(s) 381, 510, 1023, 1080, 1590
BstACI GRCGYC 1 cut(s) 1459
BstAFI CTTAAG 3 cut(s) 941, 1463, 1519
BstBAI YACGTR 1 cut(s) 778
BstBI TTCGAA 1 cut(s) 513
BstC8I GCNNGC 2 cut(s) 295, 1816
BstDEI CTNAG 9 cut(s) 198, 447, 475, 497, 657, 1381, 1391, 1737, 1835
BstEII GGTNACC 1 cut(s) 794
BstF5I GGATG 8 cut(s) 162, 311, 343, 640, 817, 1095, 1804, 1891
BstH2I RGCGCY 2 cut(s) 1233, 1462
BstHHI GCGC 4 cut(s) 159, 1232, 1461, 1874
BstKTI GATC 5 cut(s) 665, 1173, 1200, 1354, 1721
BstMAI GTCTC 5 cut(s) 36, 363, 486, 858, 910
BstMBI GATC 5 cut(s) 662, 1170, 1197, 1351, 1718
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 7 cut(s) 165, 168, 329, 575, 943, 1229, 1340
BstNI CCWGG 4 cut(s) 433, 800, 906, 1883
BstNSI RCATGY 1 cut(s) 976
BstPI GGTNACC 1 cut(s) 794
BstSCI CCNGG 6 cut(s) 332, 431, 564, 798, 904, 1881
BstV1I GCAGC 8 cut(s) 89, 92, 412, 415, 447, 450, 568, 1438
BstV2I GAAGAC 1 cut(s) 1722
BstX2I RGATCY 2 cut(s) 662, 1197
BstXI CCANNNNNNTGG 1 cut(s) 72
BstYI RGATCY 2 cut(s) 662, 1197
BstZI CGGCCG 1 cut(s) 330
Bsu15I ATCGAT 1 cut(s) 69
BsuRI GGCC 5 cut(s) 293, 326, 332, 782, 1144
BsuTUI ATCGAT 1 cut(s) 69
BtsCI GGATG 8 cut(s) 162, 311, 343, 640, 817, 1095, 1804, 1891
BtsI GCAGTG 2 cut(s) 318, 891
BtsIMutI CAGTG 5 cut(s) 112, 318, 408, 483, 891
BveI ACCTGC 2 cut(s) 624, 688
Cac8I GCNNGC 2 cut(s) 295, 1816
CaiI CAGNNNCTG 2 cut(s) 686, 890
CciI TCATGA 1 cut(s) 1068
CfoI GCGC 4 cut(s) 159, 1232, 1461, 1874
Cfr10I RCCGGY 2 cut(s) 222, 293
Cfr13I GGNCC 5 cut(s) 291, 613, 1213, 1775, 1940
ClaI ATCGAT 1 cut(s) 69
CseI GACGC 1 cut(s) 672
CviAII CATG 7 cut(s) 575, 844, 973, 1024, 1069, 1114, 1744
DdeI CTNAG 9 cut(s) 198, 447, 475, 497, 657, 1381, 1391, 1737, 1835
DinI GGCGCC 1 cut(s) 1460
DpnI GATC 5 cut(s) 664, 1172, 1199, 1353, 1720
DpnII GATC 5 cut(s) 662, 1170, 1197, 1351, 1718
DraIII CACNNNGTG 2 cut(s) 1703, 1705
EaeI YGGCCR 2 cut(s) 330, 780
EagI CGGCCG 1 cut(s) 330
Eam1104I CTCTTC 5 cut(s) 381, 510, 1023, 1080, 1590
EarI CTCTTC 5 cut(s) 381, 510, 1023, 1080, 1590
EciI GGCGGA 3 cut(s) 155, 318, 1498
Ecl136II GAGCTC 1 cut(s) 937
EclXI CGGCCG 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 485
Eco24I GRGCYC 1 cut(s) 939
Eco31I GGTCTC 2 cut(s) 36, 363
Eco47I GGWCC 4 cut(s) 613, 1213, 1775, 1940
Eco52I CGGCCG 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 937
Eco72I CACGTG 1 cut(s) 778
Eco91I GGTNACC 1 cut(s) 794
EcoICRI GAGCTC 1 cut(s) 937
EcoO109I RGGNCCY 2 cut(s) 613, 1775
EcoO65I GGTNACC 1 cut(s) 794
EcoRI GAATTC 2 cut(s) 509, 1290
EcoRII CCWGG 4 cut(s) 431, 798, 904, 1881
EcoT14I CCWWGG 1 cut(s) 485
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 939
EgeI GGCGCC 1 cut(s) 1460
EheI GGCGCC 1 cut(s) 1460
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 7 cut(s) 578, 847, 976, 1027, 1072, 1117, 1747
FaqI GGGAC 4 cut(s) 200, 285, 626, 1788
FatI CATG 7 cut(s) 574, 843, 972, 1023, 1068, 1113, 1743
FauI CCCGC 1 cut(s) 1248
FbaI TGATCA 1 cut(s) 1170
FokI GGATG 8 cut(s) 149, 318, 350, 647, 824, 1082, 1791, 1898
FriOI GRGCYC 1 cut(s) 939
FspBI CTAG 5 cut(s) 585, 1124, 1509, 1782, 1946
GlaI GCGC 4 cut(s) 158, 1231, 1460, 1873
GsuI CTGGAG 2 cut(s) 383, 1212
HaeII RGCGCY 2 cut(s) 1233, 1462
HaeIII GGCC 5 cut(s) 293, 326, 332, 782, 1144
HapII CCGG 7 cut(s) 39, 223, 294, 333, 565, 913, 1938
HgaI GACGC 1 cut(s) 672
HhaI GCGC 4 cut(s) 159, 1232, 1461, 1874
Hin1I GRCGYC 1 cut(s) 1459
Hin1II CATG 7 cut(s) 578, 847, 976, 1027, 1072, 1117, 1747
Hin6I GCGC 4 cut(s) 157, 1230, 1459, 1872
HinP1I GCGC 4 cut(s) 157, 1230, 1459, 1872
HincII GTYRAC 2 cut(s) 840, 1765
HindII GTYRAC 2 cut(s) 840, 1765
HpaI GTTAAC 1 cut(s) 840
HpaII CCGG 7 cut(s) 39, 223, 294, 333, 565, 913, 1938
HphI GGTGA 7 cut(s) 54, 110, 539, 788, 971, 1397, 1398
Hpy166II GTNNAC 3 cut(s) 840, 1420, 1765
Hpy188I TCNGA 8 cut(s) 448, 541, 899, 1162, 1170, 1289, 1339, 1659
Hpy188III TCNNGA 5 cut(s) 913, 1069, 1124, 1211, 1295
Hpy8I GTNNAC 3 cut(s) 840, 1420, 1765
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 2 cut(s) 386, 1750
HpyCH4III ACNGT 6 cut(s) 181, 1217, 1398, 1417, 1636, 1703
HpyCH4IV ACGT 3 cut(s) 777, 981, 1376
HpyCH4V TGCA 9 cut(s) 59, 265, 676, 827, 877, 976, 1301, 1366, 1454
HpyF10VI GCNNNNNNNGC 7 cut(s) 165, 168, 329, 575, 943, 1229, 1340
HpyF3I CTNAG 9 cut(s) 198, 447, 475, 497, 657, 1381, 1391, 1737, 1835
HpySE526I ACGT 3 cut(s) 777, 981, 1376
Hsp92I GRCGYC 1 cut(s) 1459
Hsp92II CATG 7 cut(s) 578, 847, 976, 1027, 1072, 1117, 1747
HspAI GCGC 4 cut(s) 157, 1230, 1459, 1872
KasI GGCGCC 1 cut(s) 1458
KflI GGGWCCC 1 cut(s) 1775
Kpn2I TCCGGA 1 cut(s) 912
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 1170
KspAI GTTAAC 1 cut(s) 840
Kzo9I GATC 5 cut(s) 662, 1170, 1197, 1351, 1718
LguI GCTCTTC 1 cut(s) 1023
LmnI GCTCC 5 cut(s) 249, 435, 942, 1227, 1910
Lsp1109I GCAGC 8 cut(s) 89, 92, 412, 415, 447, 450, 568, 1438
LweI GCATC 7 cut(s) 68, 171, 643, 836, 1163, 1288, 1330
MaeI CTAG 5 cut(s) 585, 1124, 1509, 1782, 1946
MaeII ACGT 3 cut(s) 777, 981, 1376
MaeIII GTNAC 6 cut(s) 225, 406, 532, 794, 1100, 1441
MalI GATC 5 cut(s) 664, 1172, 1199, 1353, 1720
MboI GATC 5 cut(s) 662, 1170, 1197, 1351, 1718
MfeI CAATTG 2 cut(s) 1083, 1345
MflI RGATCY 2 cut(s) 662, 1197
MhlI GDGCHC 1 cut(s) 939
Mly113I GGCGCC 1 cut(s) 1459
MlyI GAGTC 5 cut(s) 414, 1066, 1097, 1550, 1669
Mph1103I ATGCAT 1 cut(s) 61
MroI TCCGGA 1 cut(s) 912
MroNI GCCGGC 1 cut(s) 293
MseI TTAA 9 cut(s) 23, 839, 942, 987, 993, 1134, 1464, 1520, 1847
MslI CAYNNNNRTG 1 cut(s) 1673
MspA1I CMGCKG 2 cut(s) 581, 1190
MspCI CTTAAG 3 cut(s) 941, 1463, 1519
MspI CCGG 7 cut(s) 39, 223, 294, 333, 565, 913, 1938
MspR9I CCNGG 6 cut(s) 334, 433, 566, 800, 906, 1883
MunI CAATTG 2 cut(s) 1083, 1345
Mva1269I GAATGC 2 cut(s) 725, 1257
MvaI CCWGG 4 cut(s) 433, 800, 906, 1883
MwoI GCNNNNNNNGC 7 cut(s) 165, 168, 329, 575, 943, 1229, 1340
NaeI GCCGGC 1 cut(s) 295
NarI GGCGCC 1 cut(s) 1459
NciI CCSGG 2 cut(s) 334, 566
NdeII GATC 5 cut(s) 662, 1170, 1197, 1351, 1718
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 7 cut(s) 578, 847, 976, 1027, 1072, 1117, 1747
NlaIV GGNNCC 6 cut(s) 245, 614, 1279, 1460, 1776, 1777
NmeAIII GCCGAG 1 cut(s) 308
NmuCI GTSAC 4 cut(s) 225, 406, 794, 1100
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 1 cut(s) 976
NspV TTCGAA 1 cut(s) 513
PagI TCATGA 1 cut(s) 1068
PciSI GCTCTTC 1 cut(s) 1023
PcsI WCGNNNNNNNCGW 1 cut(s) 1213
PctI GAATGC 2 cut(s) 725, 1257
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 5 cut(s) 1505, 1532, 1708, 1895, 1918
PleI GAGTC 5 cut(s) 413, 1066, 1097, 1549, 1668
PluTI GGCGCC 1 cut(s) 1462
PmaCI CACGTG 1 cut(s) 778
PmlI CACGTG 1 cut(s) 778
PpsI GAGTC 5 cut(s) 413, 1066, 1097, 1549, 1668
Ppu21I YACGTR 1 cut(s) 778
PpuMI RGGWCCY 2 cut(s) 613, 1775
Psp124BI GAGCTC 1 cut(s) 939
Psp5II RGGWCCY 2 cut(s) 613, 1775
Psp6I CCWGG 4 cut(s) 431, 798, 904, 1881
PspCI CACGTG 1 cut(s) 778
PspEI GGTNACC 1 cut(s) 794
PspGI CCWGG 4 cut(s) 431, 798, 904, 1881
PspN4I GGNNCC 6 cut(s) 245, 614, 1279, 1460, 1776, 1777
PspPI GGNCC 5 cut(s) 291, 613, 1213, 1775, 1940
PspPPI RGGWCCY 2 cut(s) 613, 1775
PstNI CAGNNNCTG 2 cut(s) 686, 890
PsuI RGATCY 2 cut(s) 662, 1197
PvuII CAGCTG 1 cut(s) 1190
RseI CAYNNNNRTG 1 cut(s) 1673
SacI GAGCTC 1 cut(s) 939
SapI GCTCTTC 1 cut(s) 1023
SaqAI TTAA 9 cut(s) 23, 839, 942, 987, 993, 1134, 1464, 1520, 1847
Sau3AI GATC 5 cut(s) 662, 1170, 1197, 1351, 1718
Sau96I GGNCC 5 cut(s) 291, 613, 1213, 1775, 1940
SchI GAGTC 5 cut(s) 414, 1066, 1097, 1550, 1669
ScrFI CCNGG 6 cut(s) 334, 433, 566, 800, 906, 1883
SduI GDGCHC 1 cut(s) 939
SfaNI GCATC 7 cut(s) 68, 171, 643, 836, 1163, 1288, 1330
SfoI GGCGCC 1 cut(s) 1460
SfuI TTCGAA 1 cut(s) 513
SinI GGWCC 4 cut(s) 613, 1213, 1775, 1940
SmiMI CAYNNNNRTG 1 cut(s) 1673
SmlI CTYRAG 3 cut(s) 941, 1463, 1519
SmoI CTYRAG 3 cut(s) 941, 1463, 1519
SspDI GGCGCC 1 cut(s) 1458
SspI AATATT 1 cut(s) 1859
SspMI CTAG 5 cut(s) 585, 1124, 1509, 1782, 1946
SstI GAGCTC 1 cut(s) 939
StyD4I CCNGG 6 cut(s) 332, 431, 564, 798, 904, 1881
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 6 cut(s) 181, 1217, 1398, 1417, 1636, 1703
TaiI ACGT 3 cut(s) 780, 984, 1379
TaqI TCGA 5 cut(s) 69, 204, 513, 732, 1916
TauI GCSGC 6 cut(s) 162, 171, 326, 581, 785, 1113
TfiI GAWTC 5 cut(s) 1505, 1532, 1708, 1895, 1918
Tru1I TTAA 9 cut(s) 23, 839, 942, 987, 993, 1134, 1464, 1520, 1847
Tru9I TTAA 9 cut(s) 23, 839, 942, 987, 993, 1134, 1464, 1520, 1847
TscAI CASTG 5 cut(s) 112, 325, 415, 483, 891
TseFI GTSAC 4 cut(s) 225, 406, 794, 1100
TseI GCWGC 8 cut(s) 77, 80, 425, 428, 460, 463, 581, 1451
Tsp45I GTSAC 4 cut(s) 225, 406, 794, 1100
TspDTI ATGAA 5 cut(s) 563, 575, 1691, 1732, 1908
TspGWI ACGGA 2 cut(s) 1088, 1630
TspRI CASTG 5 cut(s) 112, 325, 415, 483, 891
Vha464I CTTAAG 3 cut(s) 941, 1463, 1519
VpaK11BI GGWCC 4 cut(s) 613, 1213, 1775, 1940
XapI RAATTY 3 cut(s) 509, 1290, 1679
XbaI TCTAGA 1 cut(s) 1123
XceI RCATGY 1 cut(s) 976
XspI CTAG 5 cut(s) 585, 1124, 1509, 1782, 1946
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.