MD16G1111200.v1.1

Ankyrin repeats (3 copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002201
Physical Location & Seq
Forward (+)
7811200 .. 7814442
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1111200.v1.1.491

Sequence Viewer

Length: 1989 bp
ATGGCACCATCTTACTTCCCTCTGAGGTGGGAGAGCACGGGAGACCAATGGTGGTATGCATCACCAATCGATTGGGCAGCAGCCAATGGCTTATATGATTTGGTCTGTGAGCTTCTTCACCTTGACACAAACCTCCTCATCAAGCTGACCTCACTACGCCGTATCCGCCGCCTCGAAACTGTCTGGGACGACGAGGCTCAGTTCGATGATGTTGCCAAATGCCGGTCACAAGTTGCCAAGAGGCTCCTCCACGAGTGCCAAATGCAGAGCGGACACAACTCTCTCATCAGGGCCGGCTACGGCGGATGGCTTCTCTACACCGCCGCCTCAGCCGGGGATGTGGGGTTTGTCAGGGAGTTGCTGGACAGAGACCCTCTTCTTGTTTTTGGAGAAGGAGAGTATGGAGTCACTGACATATTCTATGCTGCAGCCAGGAGCAAGAACTCTGAGGTTTTCAGGCTGCTGCTTGATTTCTCTGTGTCGCCAAGGTTTAGTCTTAGCAATGGAGAATTGGAAGAGAGGTTGGGTGATATTACATCGGATTTCAAGTGGGAAATGATGAACCGGGCGGTTCATGCCGCAGCTAGAGGTGGCAATTTGGAGATTTTGAGGGATTTACTTGGGGATTGTGAGGATGTATTGGCTTATAGAGATGCTCAGGGATCTACCATCTTGCATACAGCATCTGGCAGAGGGCAGGTTGAGGTGGTTGAGTATCTAATAGCATTCTTTGATACCATCACAATTGTAGATAATCAAGGGAACACAGCTTTACATGTGGCCGCTTATAGGGGTCACTTGGCTGTGGTGGAGGTCCTAATTCGTGCATCTCTCTCATTAACCACTTTGTCAAACAACTATGGAGATACCTTTCTGCATTTGGCAGTGGCTGGTTTCAGAAGCCCTGGTTTCCGGAGACTGGACAAACAGATTGAGCTTATTAAGCAATTAGTATGTGGTGATACTGTGAACATGCAGGACGTTGTTAATGTTAAGAACAATGATGGACGAACTGCTCTTCACATGGCTGTAACCGAGAACATTCAATCTAATTTGGTGGAACTCCTCATGACTGTTCCATCAATTAATTTAAACATCCGTGATTCTGGCGGCATGACCCCTCTAGATATTCTTAAGCAAAGGCCAAAGTCAGCATCTTCCGAACTTCTGATCAAGCAATTGATTTCAGCCGGAGGGATCTCTAATTGTCAGGACCGTAAAGCAAGGAGTGCCCTTGTTTCCCATTTGAGAATGCAGGGTATTGGGAGCAGTCCGGGAACCTCTTTCAGAATTCCCGATGCAGAGATATTCTTATACACAGGCATTGACAATGCTTCTGATGCCAGTTGCGATCAGTCAAGCGTGCAATTCAACACGTTCTCAGGTGAGCTAAGACAGTTTGGCTCACCCAACTCAGTAAACAATAAGAAGTCAAGTTCTGTAACTTATGCTGCAAGGCGCCTTAAGTTTCTTCTTCAATGGCGGAGGAGGAAAGAAAAAAAGGAATCTAGCATAGACGTAAGAGATAATGATTCTGAGTCATTTAGCACCTGTGTAGATTTGGACGACAATCCAATTCCACTTCGGCAGATGTATTCCAAATCTTCCTCCCTCCCAAACAACAAAAGGACAGTTTCCTTTCCGAGCCCATACACTAAAATGAAATTTACTGCTGGTTTATCACATGGTGTGATTCAGGCAAGGCCTCATTTAGCTTTTGCATCTCAATCGCACACAAGTCCTCTGTCAAGATCGTCTATGTCTTCCCCCACTTTCATGGACAAAGAGAAGGGTGTTGACATTTCGGGACCCTCTTGCTCAAATGGCAAACCACCACATCCGCAAGGGAGCTTCAAACATGATTCCTTCAATACGAAGTTAATAAACCAATATTTGTGTGTTGGCGCCCAAGGCCTGGGCATGGACGAATCAGTTTGTTCCACCTGGTCGAATCAGAATTACAAACACTCCGGTCCTCTAGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

663

Amino Acids

73.27

Weight (kDa)

6.77

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_4 PF13637 191 - 241 5.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 192 - 275 1.6e-15 Ankyrin repeats (3 copies)
Ank_2 PF12796 249 - 296 5.4e-07 Ankyrin repeats (3 copies)
Ank_5 PF13857 251 - 295 1.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 309 - 376 8.4e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 688
AccB1I GGYRCC 3 cut(s) 4, 1458, 1904
AccB7I CCANNNNNTGG 1 cut(s) 1915
AccBSI CCGCTC 1 cut(s) 270
AccIII TCCGGA 1 cut(s) 912
AclWI GGATC 2 cut(s) 670, 1205
AcoI YGGCCR 1 cut(s) 780
AcsI RAATTY 2 cut(s) 1290, 1664
AcyI GRCGYC 2 cut(s) 1459, 1905
AdeI CACNNNGTG 1 cut(s) 1688
AfiI CCNNNNNNNGG 6 cut(s) 222, 333, 789, 919, 1915, 1921
AflII CTTAAG 2 cut(s) 1133, 1463
AflIII ACRYGT 2 cut(s) 775, 1374
AgsI TTSAA 6 cut(s) 547, 1046, 1372, 1478, 1855, 1870
AjnI CCWGG 4 cut(s) 431, 904, 1914, 1943
AluBI AGCT 8 cut(s) 112, 145, 584, 770, 937, 1390, 1715, 1851
AluI AGCT 8 cut(s) 112, 145, 584, 770, 937, 1390, 1715, 1851
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 3 cut(s) 36, 363, 910
AlwI GGATC 2 cut(s) 670, 1205
AlwNI CAGNNNCTG 2 cut(s) 686, 890
Aor13HI TCCGGA 1 cut(s) 912
AoxI GGCC 5 cut(s) 291, 780, 1142, 1703, 1912
ApeKI GCWGC 8 cut(s) 77, 80, 425, 428, 460, 463, 581, 1451
ApoI RAATTY 2 cut(s) 1290, 1664
AseI ATTAAT 1 cut(s) 1086
AspLEI GCGC 2 cut(s) 1461, 1907
AspS9I GGNCC 5 cut(s) 291, 814, 1213, 1808, 1973
AsuC2I CCSGG 3 cut(s) 334, 566, 1275
AsuHPI GGTGA 6 cut(s) 54, 110, 539, 971, 1397, 1398
AvaII GGWCC 4 cut(s) 814, 1213, 1808, 1973
BaeGI GKGCMC 1 cut(s) 1234
BanI GGYRCC 3 cut(s) 4, 1458, 1904
BanII GRGCYC 1 cut(s) 1649
BauI CACGAG 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 1755
Bbv12I GWGCWC 1 cut(s) 38
BbvCI CCTCAGC 1 cut(s) 328
BbvI GCAGC 8 cut(s) 89, 92, 412, 440, 447, 450, 593, 1438
BccI CCATC 6 cut(s) 16, 300, 677, 746, 998, 1087
BceAI ACGGC 2 cut(s) 144, 316
BcgI CGANNNNNNTGC 4 cut(s) 194, 228, 1710, 1744
BciT130I CCWGG 4 cut(s) 433, 906, 1916, 1945
BciVI GTATCC 1 cut(s) 173
BclI TGATCA 1 cut(s) 1170
BcnI CCSGG 3 cut(s) 334, 566, 1275
BcoDI GTCTC 3 cut(s) 36, 363, 910
BfaI CTAG 4 cut(s) 585, 1124, 1509, 1979
BfmI CTRYAG 1 cut(s) 426
BfoI RGCGCY 2 cut(s) 1462, 1908
BfrI CTTAAG 2 cut(s) 1133, 1463
BfuAI ACCTGC 1 cut(s) 688
BfuI GTATCC 1 cut(s) 173
Bme1390I CCNGG 7 cut(s) 334, 433, 566, 906, 1275, 1916, 1945
Bme18I GGWCC 4 cut(s) 814, 1213, 1808, 1973
BmgT120I GGNCC 5 cut(s) 291, 814, 1213, 1808, 1973
BmiI GGNNCC 7 cut(s) 6, 245, 1279, 1460, 1809, 1810, 1906
BmrFI CCNGG 7 cut(s) 334, 433, 566, 906, 1275, 1916, 1945
BmsI GCATC 8 cut(s) 68, 643, 692, 836, 1163, 1288, 1330, 1730
BpiI GAAGAC 1 cut(s) 1755
Bpu10I CCTNAGC 2 cut(s) 328, 657
BpuMI CCSGG 3 cut(s) 334, 566, 1275
Bsa29I ATCGAT 1 cut(s) 69
BsaHI GRCGYC 2 cut(s) 1459, 1905
BsaI GGTCTC 2 cut(s) 36, 363
BsaJI CCNNGG 5 cut(s) 333, 485, 904, 1909, 1915
BsaWI WCCGGW 2 cut(s) 912, 1970
BsaXI ACNNNNNCTCC 2 cut(s) 1952, 1982
Bsc4I CCNNNNNNNGG 6 cut(s) 222, 333, 789, 919, 1915, 1921
Bse118I RCCGGY 2 cut(s) 222, 293
Bse1I ACTGG 2 cut(s) 924, 1344
Bse3DI GCAATG 1 cut(s) 508
BseAI TCCGGA 1 cut(s) 912
BseBI CCWGG 4 cut(s) 433, 906, 1916, 1945
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 5 cut(s) 333, 485, 904, 1909, 1915
BseGI GGATG 5 cut(s) 311, 343, 640, 1095, 1837
BseLI CCNNNNNNNGG 6 cut(s) 222, 333, 789, 919, 1915, 1921
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 8 cut(s) 14, 212, 342, 438, 671, 1395, 1428, 1527
BseNI ACTGG 2 cut(s) 924, 1344
BseRI GAGGAG 4 cut(s) 125, 236, 1055, 1501
BseSI GKGCMC 1 cut(s) 1234
BseXI GCAGC 8 cut(s) 89, 92, 412, 440, 447, 450, 593, 1438
BshFI GGCC 5 cut(s) 293, 782, 1144, 1705, 1914
BshNI GGYRCC 3 cut(s) 4, 1458, 1904
BshVI ATCGAT 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 38
BsiSI CCGG 8 cut(s) 223, 294, 333, 565, 913, 1191, 1274, 1971
BslFI GGGAC 2 cut(s) 200, 1821
BslI CCNNNNNNNGG 6 cut(s) 222, 333, 789, 919, 1915, 1921
BsmAI GTCTC 3 cut(s) 36, 363, 910
BsmFI GGGAC 2 cut(s) 200, 1821
BsmI GAATGC 2 cut(s) 725, 1257
BsnI GGCC 5 cut(s) 293, 782, 1144, 1705, 1914
Bso31I GGTCTC 2 cut(s) 36, 363
Bsp1286I GDGCHC 3 cut(s) 38, 1234, 1649
Bsp13I TCCGGA 1 cut(s) 912
Bsp143I GATC 5 cut(s) 662, 1170, 1197, 1351, 1751
BspANI GGCC 5 cut(s) 293, 782, 1144, 1705, 1914
BspCNI CTCAG 8 cut(s) 15, 211, 341, 439, 670, 1394, 1427, 1528
BspDI ATCGAT 1 cut(s) 69
BspEI TCCGGA 1 cut(s) 912
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 7 cut(s) 6, 245, 1279, 1460, 1809, 1810, 1906
BspMAI CTGCAG 1 cut(s) 430
BspMI ACCTGC 1 cut(s) 688
BspPI GGATC 2 cut(s) 670, 1205
BspQI GCTCTTC 1 cut(s) 1023
BspT107I GGYRCC 3 cut(s) 4, 1458, 1904
BspTI CTTAAG 2 cut(s) 1133, 1463
BspTNI GGTCTC 2 cut(s) 36, 363
BsrBI CCGCTC 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 508
BsrFI RCCGGY 2 cut(s) 222, 293
BsrI ACTGG 2 cut(s) 924, 1344
BssAI RCCGGY 2 cut(s) 222, 293
BssECI CCNNGG 5 cut(s) 333, 485, 904, 1909, 1915
BssMI GATC 5 cut(s) 662, 1170, 1197, 1351, 1751
BssNI GRCGYC 2 cut(s) 1459, 1905
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 2 cut(s) 485, 1909
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 4 cut(s) 433, 906, 1916, 1945
Bst4CI ACNGT 6 cut(s) 181, 967, 1075, 1217, 1398, 1633
Bst6I CTCTTC 3 cut(s) 381, 510, 1023
BstACI GRCGYC 2 cut(s) 1459, 1905
BstAFI CTTAAG 2 cut(s) 1133, 1463
BstAPI GCANNNNNTGC 1 cut(s) 1229
BstC8I GCNNGC 2 cut(s) 295, 1364
BstF5I GGATG 5 cut(s) 311, 343, 640, 1095, 1837
BstH2I RGCGCY 2 cut(s) 1462, 1908
BstHHI GCGC 2 cut(s) 1461, 1907
BstKTI GATC 5 cut(s) 665, 1173, 1200, 1354, 1754
BstMAI GTCTC 3 cut(s) 36, 363, 910
BstMBI GATC 5 cut(s) 662, 1170, 1197, 1351, 1751
BstMWI GCNNNNNNNGC 8 cut(s) 165, 329, 575, 943, 1229, 1340, 1824, 1911
BstNI CCWGG 4 cut(s) 433, 906, 1916, 1945
BstNSI RCATGY 2 cut(s) 779, 976
BstSCI CCNGG 7 cut(s) 332, 431, 564, 904, 1273, 1914, 1943
BstSFI CTRYAG 1 cut(s) 426
BstSLI GKGCMC 1 cut(s) 1234
BstV1I GCAGC 8 cut(s) 89, 92, 412, 440, 447, 450, 593, 1438
BstV2I GAAGAC 1 cut(s) 1755
BstX2I RGATCY 2 cut(s) 662, 1197
BstXI CCANNNNNNTGG 2 cut(s) 72, 1777
BstYI RGATCY 2 cut(s) 662, 1197
Bsu15I ATCGAT 1 cut(s) 69
BsuI GTATCC 1 cut(s) 173
BsuRI GGCC 5 cut(s) 293, 782, 1144, 1705, 1914
BsuTUI ATCGAT 1 cut(s) 69
BtsCI GGATG 5 cut(s) 311, 343, 640, 1095, 1837
BtsI GCAGTG 1 cut(s) 891
BtsIMutI CAGTG 2 cut(s) 408, 891
BveI ACCTGC 1 cut(s) 688
Cac8I GCNNGC 2 cut(s) 295, 1364
CaiI CAGNNNCTG 2 cut(s) 686, 890
CciI TCATGA 1 cut(s) 1068
CfoI GCGC 2 cut(s) 1461, 1907
Cfr10I RCCGGY 2 cut(s) 222, 293
Cfr13I GGNCC 5 cut(s) 291, 814, 1213, 1808, 1973
ClaI ATCGAT 1 cut(s) 69
CsiI ACCWGGT 1 cut(s) 1943
DinI GGCGCC 2 cut(s) 1460, 1906
DpnI GATC 5 cut(s) 664, 1172, 1199, 1353, 1753
DpnII GATC 5 cut(s) 662, 1170, 1197, 1351, 1751
DraI TTTAAA 1 cut(s) 1092
DraIII CACNNNGTG 1 cut(s) 1688
EaeI YGGCCR 1 cut(s) 780
Eam1104I CTCTTC 3 cut(s) 381, 510, 1023
EarI CTCTTC 3 cut(s) 381, 510, 1023
EciI GGCGGA 3 cut(s) 155, 318, 1498
Eco130I CCWWGG 2 cut(s) 485, 1909
Eco147I AGGCCT 2 cut(s) 1705, 1914
Eco24I GRGCYC 1 cut(s) 1649
Eco31I GGTCTC 2 cut(s) 36, 363
Eco47I GGWCC 4 cut(s) 814, 1213, 1808, 1973
EcoO109I RGGNCCY 2 cut(s) 814, 1808
EcoRI GAATTC 1 cut(s) 1290
EcoRII CCWGG 4 cut(s) 431, 904, 1914, 1943
EcoT14I CCWWGG 2 cut(s) 485, 1909
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 1649
EgeI GGCGCC 2 cut(s) 1460, 1906
EheI GGCGCC 2 cut(s) 1460, 1906
ErhI CCWWGG 2 cut(s) 485, 1909
FaqI GGGAC 2 cut(s) 200, 1821
FbaI TGATCA 1 cut(s) 1170
FokI GGATG 5 cut(s) 318, 350, 647, 1082, 1824
FriOI GRGCYC 1 cut(s) 1649
FspBI CTAG 4 cut(s) 585, 1124, 1509, 1979
GlaI GCGC 2 cut(s) 1460, 1906
HaeII RGCGCY 2 cut(s) 1462, 1908
HaeIII GGCC 5 cut(s) 293, 782, 1144, 1705, 1914
HapII CCGG 8 cut(s) 223, 294, 333, 565, 913, 1191, 1274, 1971
HhaI GCGC 2 cut(s) 1461, 1907
Hin1I GRCGYC 2 cut(s) 1459, 1905
Hin6I GCGC 2 cut(s) 1459, 1905
HinP1I GCGC 2 cut(s) 1459, 1905
HincII GTYRAC 1 cut(s) 1798
HindII GTYRAC 1 cut(s) 1798
HinfI GANTC 9 cut(s) 405, 1103, 1505, 1532, 1538, 1693, 1862, 1928, 1951
HpaII CCGG 8 cut(s) 223, 294, 333, 565, 913, 1191, 1274, 1971
HphI GGTGA 6 cut(s) 54, 110, 539, 971, 1397, 1398
Hpy166II GTNNAC 3 cut(s) 970, 1420, 1798
Hpy188III TCNNGA 7 cut(s) 913, 1069, 1124, 1211, 1295, 1749, 1806
Hpy8I GTNNAC 3 cut(s) 970, 1420, 1798
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 386, 1783, 1876
HpyCH4III ACNGT 6 cut(s) 181, 967, 1075, 1217, 1398, 1633
HpyCH4IV ACGT 3 cut(s) 981, 1376, 1518
HpyF10VI GCNNNNNNNGC 8 cut(s) 165, 329, 575, 943, 1229, 1340, 1824, 1911
HpySE526I ACGT 3 cut(s) 981, 1376, 1518
Hsp92I GRCGYC 2 cut(s) 1459, 1905
HspAI GCGC 2 cut(s) 1459, 1905
KasI GGCGCC 2 cut(s) 1458, 1904
KflI GGGWCCC 1 cut(s) 1808
Kpn2I TCCGGA 1 cut(s) 912
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 1170
Kzo9I GATC 5 cut(s) 662, 1170, 1197, 1351, 1751
LguI GCTCTTC 1 cut(s) 1023
LmnI GCTCC 4 cut(s) 249, 435, 1266, 1848
Lsp1109I GCAGC 8 cut(s) 89, 92, 412, 440, 447, 450, 593, 1438
LweI GCATC 8 cut(s) 68, 643, 692, 836, 1163, 1288, 1330, 1730
MabI ACCWGGT 1 cut(s) 1943
MaeI CTAG 4 cut(s) 585, 1124, 1509, 1979
MaeII ACGT 3 cut(s) 981, 1376, 1518
MaeIII GTNAC 5 cut(s) 225, 406, 794, 1030, 1441
MalI GATC 5 cut(s) 664, 1172, 1199, 1353, 1753
MbiI CCGCTC 1 cut(s) 270
MboI GATC 5 cut(s) 662, 1170, 1197, 1351, 1751
MboII GAAGA 9 cut(s) 107, 368, 527, 1010, 1149, 1463, 1466, 1596, 1755
MfeI CAATTG 2 cut(s) 744, 1178
MflI RGATCY 2 cut(s) 662, 1197
MhlI GDGCHC 3 cut(s) 38, 1234, 1649
Mly113I GGCGCC 2 cut(s) 1459, 1905
MlyI GAGTC 2 cut(s) 414, 1547
Mph1103I ATGCAT 1 cut(s) 61
MroI TCCGGA 1 cut(s) 912
MroNI GCCGGC 1 cut(s) 293
MseI TTAA 9 cut(s) 839, 942, 987, 993, 1086, 1091, 1134, 1464, 1880
MslI CAYNNNNRTG 2 cut(s) 1658, 1775
MspCI CTTAAG 2 cut(s) 1133, 1463
MspI CCGG 8 cut(s) 223, 294, 333, 565, 913, 1191, 1274, 1971
MspR9I CCNGG 7 cut(s) 334, 433, 566, 906, 1275, 1916, 1945
MunI CAATTG 2 cut(s) 744, 1178
Mva1269I GAATGC 2 cut(s) 725, 1257
MvaI CCWGG 4 cut(s) 433, 906, 1916, 1945
MwoI GCNNNNNNNGC 8 cut(s) 165, 329, 575, 943, 1229, 1340, 1824, 1911
NaeI GCCGGC 1 cut(s) 295
NarI GGCGCC 2 cut(s) 1459, 1905
NciI CCSGG 3 cut(s) 334, 566, 1275
NdeII GATC 5 cut(s) 662, 1170, 1197, 1351, 1751
NgoMIV GCCGGC 1 cut(s) 293
NlaIV GGNNCC 7 cut(s) 6, 245, 1279, 1460, 1809, 1810, 1906
NmuCI GTSAC 3 cut(s) 225, 406, 794
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 2 cut(s) 779, 976
PagI TCATGA 1 cut(s) 1068
PceI AGGCCT 2 cut(s) 1705, 1914
PciI ACATGT 1 cut(s) 775
PciSI GCTCTTC 1 cut(s) 1023
PctI GAATGC 2 cut(s) 725, 1257
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 7 cut(s) 1103, 1505, 1532, 1693, 1862, 1928, 1951
PflMI CCANNNNNTGG 1 cut(s) 1915
PfoI TCCNGGA 1 cut(s) 1273
PleI GAGTC 2 cut(s) 413, 1546
PluTI GGCGCC 2 cut(s) 1462, 1908
PpsI GAGTC 2 cut(s) 413, 1546
PpuMI RGGWCCY 2 cut(s) 814, 1808
PscI ACATGT 1 cut(s) 775
PshBI ATTAAT 1 cut(s) 1086
Psp5II RGGWCCY 2 cut(s) 814, 1808
Psp6I CCWGG 4 cut(s) 431, 904, 1914, 1943
PspGI CCWGG 4 cut(s) 431, 904, 1914, 1943
PspN4I GGNNCC 7 cut(s) 6, 245, 1279, 1460, 1809, 1810, 1906
PspPI GGNCC 5 cut(s) 291, 814, 1213, 1808, 1973
PspPPI RGGWCCY 2 cut(s) 814, 1808
PstI CTGCAG 1 cut(s) 430
PstNI CAGNNNCTG 2 cut(s) 686, 890
PsuI RGATCY 2 cut(s) 662, 1197
RseI CAYNNNNRTG 2 cut(s) 1658, 1775
SapI GCTCTTC 1 cut(s) 1023
SaqAI TTAA 9 cut(s) 839, 942, 987, 993, 1086, 1091, 1134, 1464, 1880
Sau3AI GATC 5 cut(s) 662, 1170, 1197, 1351, 1751
Sau96I GGNCC 5 cut(s) 291, 814, 1213, 1808, 1973
SchI GAGTC 2 cut(s) 414, 1547
ScrFI CCNGG 7 cut(s) 334, 433, 566, 906, 1275, 1916, 1945
SduI GDGCHC 3 cut(s) 38, 1234, 1649
SexAI ACCWGGT 1 cut(s) 1943
SfaNI GCATC 8 cut(s) 68, 643, 692, 836, 1163, 1288, 1330, 1730
SfcI CTRYAG 1 cut(s) 426
SfoI GGCGCC 2 cut(s) 1460, 1906
SinI GGWCC 4 cut(s) 814, 1213, 1808, 1973
SmiMI CAYNNNNRTG 2 cut(s) 1658, 1775
SmlI CTYRAG 2 cut(s) 1133, 1463
SmoI CTYRAG 2 cut(s) 1133, 1463
SseBI AGGCCT 2 cut(s) 1705, 1914
SspDI GGCGCC 2 cut(s) 1458, 1904
SspI AATATT 1 cut(s) 1892
SspMI CTAG 4 cut(s) 585, 1124, 1509, 1979
StuI AGGCCT 2 cut(s) 1705, 1914
StyD4I CCNGG 7 cut(s) 332, 431, 564, 904, 1273, 1914, 1943
StyI CCWWGG 2 cut(s) 485, 1909
TaaI ACNGT 6 cut(s) 181, 967, 1075, 1217, 1398, 1633
TaiI ACGT 3 cut(s) 984, 1379, 1521
TaqI TCGA 4 cut(s) 69, 174, 204, 1949
TauI GCSGC 5 cut(s) 171, 326, 581, 785, 1113
TfiI GAWTC 7 cut(s) 1103, 1505, 1532, 1693, 1862, 1928, 1951
Tru1I TTAA 9 cut(s) 839, 942, 987, 993, 1086, 1091, 1134, 1464, 1880
Tru9I TTAA 9 cut(s) 839, 942, 987, 993, 1086, 1091, 1134, 1464, 1880
TscAI CASTG 2 cut(s) 415, 891
TseFI GTSAC 3 cut(s) 225, 406, 794
TseI GCWGC 8 cut(s) 77, 80, 425, 428, 460, 463, 581, 1451
Tsp45I GTSAC 3 cut(s) 225, 406, 794
TspDTI ATGAA 4 cut(s) 563, 575, 1676, 1765
TspGWI ACGGA 1 cut(s) 1088
TspRI CASTG 2 cut(s) 415, 891
Van91I CCANNNNNTGG 1 cut(s) 1915
Vha464I CTTAAG 2 cut(s) 1133, 1463
VpaK11BI GGWCC 4 cut(s) 814, 1213, 1808, 1973
VspI ATTAAT 1 cut(s) 1086
XapI RAATTY 2 cut(s) 1290, 1664
XbaI TCTAGA 1 cut(s) 1123
XceI RCATGY 2 cut(s) 779, 976
XspI CTAG 4 cut(s) 585, 1124, 1509, 1979
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.