Rorug02G0577000

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002201
Physical Location & Seq
Forward (+)
70133961 .. 70137548
3588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0577000.1

Sequence Viewer

Length: 1299 bp
ATGGATTCAGACACAGAAGACACCAAGTTCCAAGCGACCTCAACCCAACAAGATCCATCTACGCCATCTCGCAAGCACAAGAAGAAGAAGATGAAAGTGGCCACTGAAGACTTGCTAAGCAAGCTAGAGAAGCCCCAACCAGTCCAAGCCACCATCAAAGTCATCCATGGAAACCCAGAGAGAATCGCACCTTTGGTTGGTTATTTCCCATCTGGGTTTGATCCAGACCCAAAGTCGACCAAAGTTGGGGTCTTTAGGCACAAAGAGAGAGGTGGTACAAGGCTTCAGCTTGTGGTAACCCCAAAAAAGGAGGCTAAGGTTAATTTCGTAGGAACTAGCTATGCTGGTGAGGCTCAGGCTGGGGAGTACTGCGCTTTTGGACTTGGTATTCTTGATAAGGCCACACACAAATTGAAGATTGTGCCTATTGCTAATAACAAGGTATTTAGATTAGAACCAAAAGTTCAAGGCATTGACTCCTCTAACAAGAAGCCTGCAAGTTCAGTGATTGGAGAACTTTCTACACATGAGAAGGCAGAAAAACGTATAGAACTTCATAATCGTTATGGAACAAAGAAGTCAATACGGCAGAACAAAAAAATGCAAACCTTGAATCACGGAGATGATCCTGATGCTCAAAATGATTTGGATGCAATCAAACAAGGTATGACAAACAGGGAGGATTTGTTAGAAGCTTCCAAAGGCCATAACGATCGCAATACCCCACCATTTAATGCTTCTGCCACCACTCCCCAGGAGGCTTATCCACTGGACAAGATCATCACCACAGGAGAGTGGAAATTCCTACCAGATATCTATAAGCTTTTGGAAGGGGGATCAGACCTTCCCCAGTTTGTTAGCAACCGAATCCATAAACTTCAGCAGATTGAGGATGAAGACAAGAAGAATAAGCTTTCTTGTATTTTCTCATATATTACGCATCTTATAAAGTTCAAAGATCTGCATTCCATGGAGGGGTTTTCCTCTGCACAGGACCACAGATTCCCAAGCATTATAAGGGACAAATTTTCAAGCATGTTTGATGCTGTGCCGAGAAGGCTATCAACCGAGAAAAGTAATCTTCTCATTAGTTATGTCCTGGTGCTCACTCTTTTTGCGGATGAATTCCAAACAAGTTTGACAGACATAACGAAGGATCTGAAAATGAGTCATTTAGCTCTGAGGACGCATTATGAGTCCTTGGGTTGCAAGGTGTCTAAGAAAAACAATTTATCAATGGTTACCCTTCCTGTCCCCCTCCAATTTCCAACAGTGAAGCGTAAGCGACCCAATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

432

Amino Acids

48.68

Weight (kDa)

9.49

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_I_A49 PF06870 77 - 423 2.3e-48 A49-like RNA polymerase I associated factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 947, 1016
AccI GTMKAC 1 cut(s) 236
AciI CCGC 1 cut(s) 1118
AclWI GGATC 5 cut(s) 47, 215, 620, 844, 1164
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 3 cut(s) 800, 1025, 1124
AcuI CTGAAG 3 cut(s) 126, 269, 863
AfaI GTAC 2 cut(s) 277, 368
AfiI CCNNNNNNNGG 4 cut(s) 197, 246, 307, 975
AgsI TTSAA 5 cut(s) 415, 467, 613, 955, 1032
AhdI GACNNNNNGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 753, 1098
AluBI AGCT 7 cut(s) 124, 289, 339, 695, 823, 913, 1178
AluI AGCT 7 cut(s) 124, 289, 339, 695, 823, 913, 1178
Alw21I GWGCWC 1 cut(s) 1107
AlwI GGATC 5 cut(s) 47, 215, 620, 844, 1164
AoxI GGCC 3 cut(s) 99, 399, 703
ApoI RAATTY 3 cut(s) 800, 1025, 1124
ArsI GACNNNNNNTTYG 2 cut(s) 234, 266
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 1 cut(s) 994
AsuHPI GGTGA 2 cut(s) 359, 775
AvaII GGWCC 1 cut(s) 994
BalI TGGCCA 1 cut(s) 101
BbsI GAAGAC 3 cut(s) 24, 114, 903
Bbv12I GWGCWC 1 cut(s) 1107
BccI CCATC 4 cut(s) 64, 73, 161, 217
BceAI ACGGC 1 cut(s) 602
BciT130I CCWGG 2 cut(s) 755, 1100
BfaI CTAG 2 cut(s) 125, 336
BglI GCCNNNNNGGC 1 cut(s) 1057
BglII AGATCT 1 cut(s) 958
BlpI GCTNAGC 1 cut(s) 116
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 2 cut(s) 755, 1100
Bme18I GGWCC 1 cut(s) 994
BmeRI GACNNNNNGTC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 994
BmrFI CCNGG 2 cut(s) 755, 1100
BmrI ACTGGG 1 cut(s) 844
BmsI GCATC 4 cut(s) 622, 640, 949, 1033
BmuI ACTGGG 1 cut(s) 844
BpiI GAAGAC 3 cut(s) 24, 114, 903
Bpu10I CCTNAGC 2 cut(s) 315, 354
Bpu1102I GCTNAGC 1 cut(s) 116
BsaJI CCNNGG 4 cut(s) 166, 753, 969, 1200
BsaXI ACNNNNNCTCC 2 cut(s) 671, 701
Bsc4I CCNNNNNNNGG 4 cut(s) 197, 246, 307, 975
Bse1I ACTGG 3 cut(s) 140, 774, 850
BseBI CCWGG 2 cut(s) 755, 1100
BseDI CCNNGG 4 cut(s) 166, 753, 969, 1200
BseGI GGATG 4 cut(s) 162, 655, 898, 1126
BseLI CCNNNNNNNGG 4 cut(s) 197, 246, 307, 975
BseMII CTCAG 2 cut(s) 368, 1172
BseNI ACTGG 3 cut(s) 140, 774, 850
BseRI GAGGAG 1 cut(s) 469
BseYI CCCAGC 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 972
Bsh1285I CGRYCG 1 cut(s) 715
BshFI GGCC 3 cut(s) 101, 401, 705
BsiEI CGRYCG 1 cut(s) 715
BsiHKAI GWGCWC 1 cut(s) 1107
BslFI GGGAC 2 cut(s) 1034, 1238
BslI CCNNNNNNNGG 4 cut(s) 197, 246, 307, 975
BsmFI GGGAC 2 cut(s) 1034, 1238
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 3 cut(s) 101, 401, 705
Bsp1286I GDGCHC 1 cut(s) 1107
Bsp143I GATC 8 cut(s) 52, 220, 625, 712, 777, 836, 958, 1156
Bsp1720I GCTNAGC 1 cut(s) 116
Bsp19I CCATGG 2 cut(s) 166, 969
BspACI CCGC 1 cut(s) 1118
BspANI GGCC 3 cut(s) 101, 401, 705
BspCNI CTCAG 2 cut(s) 367, 1173
BspPI GGATC 5 cut(s) 47, 215, 620, 844, 1164
BsrI ACTGG 3 cut(s) 140, 774, 850
BssECI CCNNGG 4 cut(s) 166, 753, 969, 1200
BssMI GATC 8 cut(s) 52, 220, 625, 712, 777, 836, 958, 1156
BssT1I CCWWGG 3 cut(s) 166, 969, 1200
Bst2UI CCWGG 2 cut(s) 755, 1100
Bst4CI ACNGT 1 cut(s) 1273
BstC8I GCNNGC 3 cut(s) 74, 122, 495
BstDEI CTNAG 5 cut(s) 116, 315, 354, 1181, 1218
BstDSI CCRYGG 2 cut(s) 166, 969
BstEII GGTNACC 2 cut(s) 295, 1240
BstF5I GGATG 4 cut(s) 162, 655, 898, 1126
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 8 cut(s) 55, 223, 628, 715, 780, 839, 961, 1159
BstMBI GATC 8 cut(s) 52, 220, 625, 712, 777, 836, 958, 1156
BstMCI CGRYCG 1 cut(s) 715
BstMWI GCNNNNNNNGC 4 cut(s) 121, 130, 350, 1057
BstNI CCWGG 2 cut(s) 755, 1100
BstNSI RCATGY 1 cut(s) 1039
BstPI GGTNACC 2 cut(s) 295, 1240
BstSCI CCNGG 2 cut(s) 753, 1098
BstV2I GAAGAC 3 cut(s) 24, 114, 903
BstX2I RGATCY 3 cut(s) 52, 958, 1156
BstYI RGATCY 3 cut(s) 52, 958, 1156
BsuRI GGCC 3 cut(s) 101, 401, 705
BtgI CCRYGG 2 cut(s) 166, 969
BtsCI GGATG 4 cut(s) 162, 655, 898, 1126
BtsIMutI CAGTG 4 cut(s) 102, 510, 767, 1278
Cac8I GCNNGC 3 cut(s) 74, 122, 495
CfoI GCGC 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 994
CseI GACGC 1 cut(s) 1195
Csp6I GTAC 2 cut(s) 276, 367
CviAII CATG 4 cut(s) 167, 527, 970, 1036
CviQI GTAC 2 cut(s) 276, 367
DdeI CTNAG 5 cut(s) 116, 315, 354, 1181, 1218
DpnI GATC 8 cut(s) 54, 222, 627, 714, 779, 838, 960, 1158
DpnII GATC 8 cut(s) 52, 220, 625, 712, 777, 836, 958, 1156
DriI GACNNNNNGTC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 99
Eam1105I GACNNNNNGTC 1 cut(s) 232
Eco130I CCWWGG 3 cut(s) 166, 969, 1200
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 1 cut(s) 994
Eco57I CTGAAG 3 cut(s) 126, 269, 863
Eco91I GGTNACC 2 cut(s) 295, 1240
EcoO65I GGTNACC 2 cut(s) 295, 1240
EcoRI GAATTC 1 cut(s) 1124
EcoRII CCWGG 2 cut(s) 753, 1098
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 3 cut(s) 166, 969, 1200
ErhI CCWWGG 3 cut(s) 166, 969, 1200
FaeI CATG 4 cut(s) 170, 530, 973, 1039
FaqI GGGAC 2 cut(s) 1034, 1238
FatI CATG 4 cut(s) 166, 526, 969, 1035
FblI GTMKAC 1 cut(s) 236
FokI GGATG 4 cut(s) 149, 662, 905, 1133
FspBI CTAG 2 cut(s) 125, 336
GlaI GCGC 1 cut(s) 373
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 3 cut(s) 101, 401, 705
HgaI GACGC 1 cut(s) 1195
HhaI GCGC 1 cut(s) 374
Hin1II CATG 4 cut(s) 170, 530, 973, 1039
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HindIII AAGCTT 3 cut(s) 693, 821, 911
HinfI GANTC 8 cut(s) 5, 183, 476, 613, 867, 1002, 1168, 1196
HphI GGTGA 2 cut(s) 359, 775
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 4 cut(s) 10, 841, 1161, 1182
Hpy188III TCNNGA 3 cut(s) 224, 392, 629
Hpy8I GTNNAC 1 cut(s) 237
HpyAV CCTTC 6 cut(s) 526, 824, 854, 1050, 1147, 1256
HpyCH4III ACNGT 1 cut(s) 1273
HpyCH4IV ACGT 1 cut(s) 544
HpyCH4V TGCA 6 cut(s) 497, 604, 653, 964, 989, 1209
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 130, 350, 1057
HpyF3I CTNAG 5 cut(s) 116, 315, 354, 1181, 1218
HpySE526I ACGT 1 cut(s) 544
Hsp92II CATG 4 cut(s) 170, 530, 973, 1039
HspAI GCGC 1 cut(s) 372
Kzo9I GATC 8 cut(s) 52, 220, 625, 712, 777, 836, 958, 1156
LweI GCATC 4 cut(s) 622, 640, 949, 1033
MaeI CTAG 2 cut(s) 125, 336
MaeII ACGT 1 cut(s) 544
MaeIII GTNAC 2 cut(s) 295, 1240
MalI GATC 8 cut(s) 54, 222, 627, 714, 779, 838, 960, 1158
MboI GATC 8 cut(s) 52, 220, 625, 712, 777, 836, 958, 1156
MboII GAAGA 9 cut(s) 29, 94, 97, 100, 119, 427, 908, 916, 1073
MflI RGATCY 3 cut(s) 52, 958, 1156
MhlI GDGCHC 1 cut(s) 1107
MlsI TGGCCA 1 cut(s) 101
MluCI AATT 7 cut(s) 322, 410, 800, 1025, 1124, 1228, 1262
MluNI TGGCCA 1 cut(s) 101
MlyI GAGTC 3 cut(s) 470, 1177, 1205
MmeI TCCRAC 1 cut(s) 1292
Mox20I TGGCCA 1 cut(s) 101
MscI TGGCCA 1 cut(s) 101
MseI TTAA 2 cut(s) 321, 732
MslI CAYNNNNRTG 1 cut(s) 621
Msp20I TGGCCA 1 cut(s) 101
MspR9I CCNGG 2 cut(s) 755, 1100
Mva1269I GAATGC 1 cut(s) 964
MvaI CCWGG 2 cut(s) 755, 1100
MwoI GCNNNNNNNGC 4 cut(s) 121, 130, 350, 1057
NcoI CCATGG 2 cut(s) 166, 969
NdeII GATC 8 cut(s) 52, 220, 625, 712, 777, 836, 958, 1156
NlaIII CATG 4 cut(s) 170, 530, 973, 1039
NmeAIII GCCGAG 1 cut(s) 1077
NspI RCATGY 1 cut(s) 1039
PctI GAATGC 1 cut(s) 964
PfeI GAWTC 5 cut(s) 5, 183, 613, 867, 1002
Ple19I CGATCG 1 cut(s) 715
PleI GAGTC 3 cut(s) 470, 1176, 1204
PpsI GAGTC 3 cut(s) 470, 1176, 1204
PsiI TTATAA 2 cut(s) 947, 1016
Psp6I CCWGG 2 cut(s) 753, 1098
PspEI GGTNACC 2 cut(s) 295, 1240
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 2 cut(s) 753, 1098
PspPI GGNCC 1 cut(s) 994
PsuI RGATCY 3 cut(s) 52, 958, 1156
PvuI CGATCG 1 cut(s) 715
RsaI GTAC 2 cut(s) 277, 368
RsaNI GTAC 2 cut(s) 276, 367
RseI CAYNNNNRTG 1 cut(s) 621
SalI GTCGAC 1 cut(s) 235
SaqAI TTAA 2 cut(s) 321, 732
Sau3AI GATC 8 cut(s) 52, 220, 625, 712, 777, 836, 958, 1156
Sau96I GGNCC 1 cut(s) 994
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 3 cut(s) 470, 1177, 1205
ScrFI CCNGG 2 cut(s) 755, 1100
SduI GDGCHC 1 cut(s) 1107
SfaNI GCATC 4 cut(s) 622, 640, 949, 1033
SinI GGWCC 1 cut(s) 994
SmiMI CAYNNNNRTG 1 cut(s) 621
Sse9I AATT 7 cut(s) 322, 410, 800, 1025, 1124, 1228, 1262
SsiI CCGC 1 cut(s) 1118
SspMI CTAG 2 cut(s) 125, 336
StyD4I CCNGG 2 cut(s) 753, 1098
StyI CCWWGG 3 cut(s) 166, 969, 1200
TaaI ACNGT 1 cut(s) 1273
TaiI ACGT 1 cut(s) 547
TaqI TCGA 1 cut(s) 236
TasI AATT 7 cut(s) 322, 410, 800, 1025, 1124, 1228, 1262
TatI WGTACW 1 cut(s) 366
TfiI GAWTC 5 cut(s) 5, 183, 613, 867, 1002
Tru1I TTAA 2 cut(s) 321, 732
Tru9I TTAA 2 cut(s) 321, 732
TscAI CASTG 4 cut(s) 109, 510, 774, 1278
TspDTI ATGAA 4 cut(s) 107, 545, 909, 1137
TspGWI ACGGA 1 cut(s) 633
TspRI CASTG 4 cut(s) 109, 510, 774, 1278
VpaK11BI GGWCC 1 cut(s) 994
XapI RAATTY 3 cut(s) 800, 1025, 1124
XceI RCATGY 1 cut(s) 1039
XmiI GTMKAC 1 cut(s) 236
XspI CTAG 2 cut(s) 125, 336
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.