MD09G1016700.v1.1

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002201
Physical Location & Seq
Reverse (-)
1044397 .. 1048798
4402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1016700.v1.1.491

Sequence Viewer

Length: 2055 bp
ATGCCTCCTACATATTTTCCTCTCCGGTGGGAAAGCACCGGAGACCAATGGTGGTACGCGTCTCCGATCGATTACGCAGCCGCCAACGGCCACTACGACTTGGTTCGAGAGCTTCTCCGCATAGACGGCAACCACCTCATCAAGCTCACATCTCTCCGCCGTATTCGCCGCCTCGAGGCAGTGTGGGACGACGAAGCTCAGTATGATGATGTAGCCAAGTGTCGTTCGTGCGTTGCGCGAAAGCTCTTCAACGAATGCGAGTCCAAGAAAGGAAAACCAAACTCTCTCATAAGGGCTGGATACGGCGGTTGGCTTATGTACACCGCTGCCTCCGCCGGGGAGCTTGGTTTTGTCCAAGAACTGCTCGAGAGAAACCCTCTGCTTGTTTTCGGGGAAGGAGAATACGGGGTGACTGATATTTTATATGCTGCTGCGAAGAGCAAGAATGTTGAGATTTTCAGGCTGCTTTTTGACTTTGCAGTTTCGCCGAGGTTTATGACGGGGAGTGGTGGAGAGCTGGAAGAGCACATTGGGGAGATACCTTGTGTTTATAAACGGGAGATGGTTAACAGGGCGGTTCATGCGGCGGCGAGAGGCGGAAATTTGAGTGTTTTGAAGGAGCTTCTTTCGGATTGTGAGGATGTTTTGGCTTATGGGGATATTCAGGGATCAACTGTGCTACATGCAGCGGCTGGGAAAGGGCGGGTAGAGGTAGTGAAGTATCTTGTAGCATCCTTTGATATCATAAACTCCACTGATCTCCAAGGCGACACAGCGTTACATGTTGCAGCTTCAAGGGGCCAACTAGCCGCAGCCGAAGCGCTTATATTGGCATCTCCGTCGTCTATTAATGCACGAAACAACTCCGGAGAAACGTTTCTCCACAAGGCTATATCCGGCTTCCAAAGCCCTGCCTTCCGAAGACTGGACAGACAAATTCAGCTTCTGAAGCAACTGGTGTGCGAGAAAGCTTTCAATATCGAACAAATCATCAATGCAAAAAACAACGAAGGACGGACTGCCCTTCACACAGCAATCATTGGGGATGTGCATTCCGATTTAGTCCAACTCCTGATGATTGTGCAATCGATTGATGTGAACGCCCGCGATGTGGATGGCATGACAGCGCTAGATTACATTAGGCAATGGCCTCGTTCGGCATCATCTGATATACTAATCAGGCAGTTGATTTCGGCTGGGGGAATATTTGGTTGTCAGGATTACAATGCAAGAAAAGCGATTGCTTCACGCTTGAAGACACAAGGCGATGGAGGTAGTCCCGGAACATCATTTAGAATCTCTGACACGGAAATCTTCTTGTATACTGGCATTGAAAATGTAACAGATGCTACTGCTGATCGCCGTAGCATAGGAATCAATTCATCTCCACCCGAACTGATCAGTCCGTATCCAACTGCTGAAAATAACGGCAGCTCATTCGCTAGTAAAAAACCTGGTTCTGTAAATAATGCAGCAGCACAACTAAAACGTGTCGTAGGCTGGCCACAAATGAAACCGAAAAAGCACGAAAGGTTCAAGAAATCGGTTGATTTGGGATCAGTAGAGTCTATTAAAATATGCAACAAAAGCTCAGATGATGCAGCTCCGACCCCGCTTCGACAGAGATTTTCGAGGCCCTCAGTCTCATCAGTTCCCAGCAACAAGCGGACACTTTCGGTGAGGAGCAACCAGTCAAGTCCATCAGCGAAAAAGAGGTTTGCTTGTGGCATAAGGCGTGGCGTAATGCAGGCAATTCCGCACATAACTGTGCCACGTCGTTCTCGGTCTAGTTCATTTTCGAAATCATCCTCAATTTCTTCACCTACTTCTCTAGATATTCAGAAAAACGTTTGCATGGAAACTGACATTGCAGGACCGTCTTGGTCGACTGAAGTAGCTGATGACGAACCGCCGAATTTGGCGAAACAAGGCTCGCTTAATAAGAGGCCGAGGAGACAGTATTTCTGTTTTGGTGCATCACGATTGTCTGTGAAAAGCCCAGTTAACAGGCAGCAGCAAAGTTTCAAGCATTCTCCTGTTATTTCAGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

685

Amino Acids

75.04

Weight (kDa)

9.28

Isoelectric Point (pI)

54.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 192 - 245 1.2e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 193 - 243 6.4e-06 Ankyrin repeats (many copies)
Ank_4 PF13637 227 - 276 1.5e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 249 - 297 2.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 250 - 300 8e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 331 - 379 2.7e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AbsI CCTCGAGG 1 cut(s) 173
AccI GTMKAC 2 cut(s) 1322, 1886
AccII CGCG 3 cut(s) 59, 238, 1107
AccIII TCCGGA 1 cut(s) 866
AclI AACGTT 2 cut(s) 875, 1848
AclWI GGATC 2 cut(s) 676, 1564
AcoI YGGCCR 2 cut(s) 88, 1502
AcsI RAATTY 3 cut(s) 601, 936, 1915
AcuI CTGAAG 2 cut(s) 968, 1911
AfaI GTAC 2 cut(s) 56, 320
AfeI AGCGCT 2 cut(s) 822, 1128
AfiI CCNNNNNNNGG 4 cut(s) 175, 336, 925, 1111
AflIII ACRYGT 3 cut(s) 57, 781, 1489
AgsI TTSAA 8 cut(s) 250, 616, 795, 976, 1255, 1334, 1537, 2026
AjiI CACGTC 1 cut(s) 1775
AjnI CCWGG 1 cut(s) 1453
AjuI GAANNNNNNNTTGG 2 cut(s) 513, 545
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 4 cut(s) 36, 66, 1648, 1948
AlwI GGATC 2 cut(s) 676, 1564
AlwNI CAGNNNCTG 2 cut(s) 692, 946
Ama87I CYCGRG 2 cut(s) 173, 365
Aor13HI TCCGGA 1 cut(s) 866
Aor51HI AGCGCT 2 cut(s) 822, 1128
AoxI GGCC 6 cut(s) 88, 799, 1148, 1502, 1634, 1946
ApoI RAATTY 3 cut(s) 601, 936, 1915
AseI ATTAAT 1 cut(s) 849
Asp700I GAANNNNTTC 3 cut(s) 876, 971, 1378
AspLEI GCGC 3 cut(s) 238, 823, 1129
AspS9I GGNCC 3 cut(s) 799, 1635, 1874
AsuC2I CCSGG 2 cut(s) 337, 1281
AsuHPI GGTGA 3 cut(s) 421, 1690, 1812
AsuII TTCGAA 1 cut(s) 1799
AvaI CYCGRG 2 cut(s) 173, 365
AvaII GGWCC 1 cut(s) 1874
BaeI ACNNNNGTAYC 2 cut(s) 292, 325
BalI TGGCCA 1 cut(s) 1504
BbsI GAAGAC 2 cut(s) 928, 1262
Bbv12I GWGCWC 1 cut(s) 528
BccI CCATC 4 cut(s) 556, 1109, 1262, 1708
BceAI ACGGC 6 cut(s) 103, 142, 144, 319, 1347, 1444
BcgI CGANNNNNNTGC 6 cut(s) 822, 856, 1133, 1167, 1420, 1454
BciT130I CCWGG 1 cut(s) 1455
BciVI GTATCC 2 cut(s) 293, 1419
BclI TGATCA 1 cut(s) 1398
BcnI CCSGG 2 cut(s) 337, 1281
BcoDI GTCTC 4 cut(s) 36, 66, 1648, 1948
BfaI CTAG 5 cut(s) 806, 1130, 1443, 1788, 1832
BfoI RGCGCY 2 cut(s) 824, 1130
BfuI GTATCC 2 cut(s) 293, 1419
Bme1390I CCNGG 3 cut(s) 337, 1281, 1455
Bme18I GGWCC 1 cut(s) 1874
BmeT110I CYCGRG 2 cut(s) 173, 365
BmgBI CACGTC 1 cut(s) 1775
BmgT120I GGNCC 3 cut(s) 799, 1635, 1874
BmiI GGNNCC 1 cut(s) 800
BmrFI CCNGG 3 cut(s) 337, 1281, 1455
BmrI ACTGGG 1 cut(s) 1994
BmsI GCATC 6 cut(s) 740, 842, 1169, 1336, 1588, 1985
BmuI ACTGGG 1 cut(s) 1994
BpiI GAAGAC 2 cut(s) 928, 1262
BplI GAGNNNNNCTC 4 cut(s) 99, 131, 361, 393
Bpu14I TTCGAA 1 cut(s) 1799
BpuMI CCSGG 2 cut(s) 337, 1281
Bsa29I ATCGAT 2 cut(s) 69, 1088
BsaI GGTCTC 1 cut(s) 36
BsaJI CCNNGG 4 cut(s) 336, 488, 763, 1949
BsaWI WCCGGW 3 cut(s) 24, 38, 866
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 336, 925, 1111
Bse1I ACTGG 5 cut(s) 930, 960, 1330, 1690, 2000
Bse3DI GCAATG 2 cut(s) 1151, 1866
BseAI TCCGGA 1 cut(s) 866
BseBI CCWGG 1 cut(s) 1455
BseCI ATCGAT 2 cut(s) 69, 1088
BseDI CCNNGG 4 cut(s) 336, 488, 763, 1949
BseGI GGATG 5 cut(s) 646, 731, 1051, 1120, 1805
BseLI CCNNNNNNNGG 4 cut(s) 175, 336, 925, 1111
BseMI GCAATG 2 cut(s) 1151, 1866
BseMII CTCAG 3 cut(s) 212, 1605, 1653
BseNI ACTGG 5 cut(s) 930, 960, 1330, 1690, 2000
BseRI GAGGAG 2 cut(s) 1696, 1966
BseYI CCCAGC 3 cut(s) 692, 1196, 1655
Bsh1236I CGCG 3 cut(s) 59, 238, 1107
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 6 cut(s) 90, 801, 1150, 1504, 1636, 1948
BshVI ATCGAT 2 cut(s) 69, 1088
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 528
BsiHKCI CYCGRG 2 cut(s) 173, 365
BsiSI CCGG 6 cut(s) 25, 39, 336, 867, 897, 1281
BslFI GGGAC 2 cut(s) 200, 1263
BslI CCNNNNNNNGG 4 cut(s) 175, 336, 925, 1111
BsmAI GTCTC 4 cut(s) 36, 66, 1648, 1948
BsmBI CGTCTC 1 cut(s) 66
BsmFI GGGAC 2 cut(s) 200, 1263
BsmI GAATGC 3 cut(s) 260, 1051, 2029
BsnI GGCC 6 cut(s) 90, 801, 1150, 1504, 1636, 1948
Bso31I GGTCTC 1 cut(s) 36
BsoBI CYCGRG 2 cut(s) 173, 365
Bsp119I TTCGAA 1 cut(s) 1799
Bsp1286I GDGCHC 1 cut(s) 528
Bsp13I TCCGGA 1 cut(s) 866
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 6 cut(s) 66, 668, 757, 1357, 1398, 1556
BspANI GGCC 6 cut(s) 90, 801, 1150, 1504, 1636, 1948
BspCNI CTCAG 3 cut(s) 211, 1604, 1652
BspDI ATCGAT 2 cut(s) 69, 1088
BspEI TCCGGA 1 cut(s) 866
BspFNI CGCG 3 cut(s) 59, 238, 1107
BspLI GGNNCC 1 cut(s) 800
BspPI GGATC 2 cut(s) 676, 1564
BspQI GCTCTTC 3 cut(s) 251, 431, 516
BspT104I TTCGAA 1 cut(s) 1799
BspTNI GGTCTC 1 cut(s) 36
BsrDI GCAATG 2 cut(s) 1151, 1866
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 5 cut(s) 930, 960, 1330, 1690, 2000
BssECI CCNNGG 4 cut(s) 336, 488, 763, 1949
BssMI GATC 6 cut(s) 66, 668, 757, 1357, 1398, 1556
BssNAI GTATAC 1 cut(s) 1323
BssT1I CCWWGG 1 cut(s) 763
Bst1107I GTATAC 1 cut(s) 1323
Bst2UI CCWGG 1 cut(s) 1455
Bst4CI ACNGT 4 cut(s) 676, 1768, 1878, 1959
Bst6I CTCTTC 3 cut(s) 251, 431, 516
BstAUI TGTACA 1 cut(s) 318
BstBI TTCGAA 1 cut(s) 1799
BstC8I GCNNGC 4 cut(s) 1105, 1502, 1749, 1934
BstDEI CTNAG 3 cut(s) 198, 1591, 1639
BstF5I GGATG 5 cut(s) 646, 731, 1051, 1120, 1805
BstFNI CGCG 3 cut(s) 59, 238, 1107
BstH2I RGCGCY 2 cut(s) 824, 1130
BstHHI GCGC 3 cut(s) 238, 823, 1129
BstKTI GATC 6 cut(s) 69, 671, 760, 1360, 1401, 1559
BstMAI GTCTC 4 cut(s) 36, 66, 1648, 1948
BstMBI GATC 6 cut(s) 66, 668, 757, 1357, 1398, 1556
BstMCI CGRYCG 1 cut(s) 69
BstNI CCWGG 1 cut(s) 1455
BstNSI RCATGY 2 cut(s) 686, 785
BstSCI CCNGG 3 cut(s) 335, 1279, 1453
BstUI CGCG 3 cut(s) 59, 238, 1107
BstV2I GAAGAC 2 cut(s) 928, 1262
BstZ17I GTATAC 1 cut(s) 1323
Bsu15I ATCGAT 2 cut(s) 69, 1088
BsuI GTATCC 2 cut(s) 293, 1419
BsuRI GGCC 6 cut(s) 90, 801, 1150, 1504, 1636, 1948
BsuTUI ATCGAT 2 cut(s) 69, 1088
BtgZI GCGATG 2 cut(s) 1122, 1281
BtrI CACGTC 1 cut(s) 1775
BtsCI GGATG 5 cut(s) 646, 731, 1051, 1120, 1805
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 2 cut(s) 186, 753
Cac8I GCNNGC 4 cut(s) 1105, 1502, 1749, 1934
CaiI CAGNNNCTG 2 cut(s) 692, 946
CfoI GCGC 3 cut(s) 238, 823, 1129
Cfr13I GGNCC 3 cut(s) 799, 1635, 1874
ClaI ATCGAT 2 cut(s) 69, 1088
CseI GACGC 1 cut(s) 48
CsiI ACCWGGT 1 cut(s) 1453
Csp6I GTAC 2 cut(s) 55, 319
CviAII CATG 5 cut(s) 581, 683, 782, 1120, 1855
CviQI GTAC 2 cut(s) 55, 319
DdeI CTNAG 3 cut(s) 198, 1591, 1639
DpnI GATC 6 cut(s) 68, 670, 759, 1359, 1400, 1558
DpnII GATC 6 cut(s) 66, 668, 757, 1357, 1398, 1556
EaeI YGGCCR 2 cut(s) 88, 1502
Eam1104I CTCTTC 3 cut(s) 251, 431, 516
EarI CTCTTC 3 cut(s) 251, 431, 516
EciI GGCGGA 3 cut(s) 146, 322, 612
Eco130I CCWWGG 1 cut(s) 763
Eco31I GGTCTC 1 cut(s) 36
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 1 cut(s) 1874
Eco47III AGCGCT 2 cut(s) 822, 1128
Eco57I CTGAAG 2 cut(s) 968, 1911
Eco88I CYCGRG 2 cut(s) 173, 365
EcoO109I RGGNCCY 1 cut(s) 1635
EcoRII CCWGG 1 cut(s) 1453
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 763
ErhI CCWWGG 1 cut(s) 763
Esp3I CGTCTC 1 cut(s) 66
FaeI CATG 5 cut(s) 584, 686, 785, 1123, 1858
FalI AAGNNNNNCTT 2 cut(s) 1920, 1952
FaqI GGGAC 2 cut(s) 200, 1263
FatI CATG 5 cut(s) 580, 682, 781, 1119, 1854
FauI CCCGC 3 cut(s) 696, 1112, 1620
FbaI TGATCA 1 cut(s) 1398
FblI GTMKAC 2 cut(s) 1322, 1886
FokI GGATG 5 cut(s) 653, 718, 1058, 1127, 1792
FspBI CTAG 5 cut(s) 806, 1130, 1443, 1788, 1832
GlaI GCGC 3 cut(s) 237, 822, 1128
GsaI CCCAGC 3 cut(s) 696, 1200, 1659
HaeII RGCGCY 2 cut(s) 824, 1130
HaeIII GGCC 6 cut(s) 90, 801, 1150, 1504, 1636, 1948
HapII CCGG 6 cut(s) 25, 39, 336, 867, 897, 1281
HgaI GACGC 1 cut(s) 48
HhaI GCGC 3 cut(s) 238, 823, 1129
Hin1II CATG 5 cut(s) 584, 686, 785, 1123, 1858
Hin6I GCGC 3 cut(s) 236, 821, 1127
HinP1I GCGC 3 cut(s) 236, 821, 1127
HincII GTYRAC 3 cut(s) 568, 1887, 2005
HindII GTYRAC 3 cut(s) 568, 1887, 2005
HindIII AAGCTT 1 cut(s) 969
HinfI GANTC 4 cut(s) 260, 1296, 1374, 1565
HpaI GTTAAC 2 cut(s) 568, 2005
HpaII CCGG 6 cut(s) 25, 39, 336, 867, 897, 1281
HphI GGTGA 3 cut(s) 421, 1690, 1812
Hpy166II GTNNAC 6 cut(s) 321, 568, 1099, 1323, 1887, 2005
Hpy188III TCNNGA 8 cut(s) 107, 367, 867, 1072, 1217, 1537, 1832, 1980
Hpy8I GTNNAC 6 cut(s) 321, 568, 1099, 1323, 1887, 2005
Hpy99I CGWCG 3 cut(s) 194, 844, 1779
HpyAV CCTTC 5 cut(s) 389, 610, 925, 1004, 1034
HpyCH4III ACNGT 4 cut(s) 676, 1768, 1878, 1959
HpyCH4IV ACGT 4 cut(s) 875, 1489, 1774, 1848
HpyF3I CTNAG 3 cut(s) 198, 1591, 1639
HpySE526I ACGT 4 cut(s) 875, 1489, 1774, 1848
Hsp92II CATG 5 cut(s) 584, 686, 785, 1123, 1858
HspAI GCGC 3 cut(s) 236, 821, 1127
Kpn2I TCCGGA 1 cut(s) 866
Ksp22I TGATCA 1 cut(s) 1398
KspAI GTTAAC 2 cut(s) 568, 2005
Kzo9I GATC 6 cut(s) 66, 668, 757, 1357, 1398, 1556
LguI GCTCTTC 3 cut(s) 251, 431, 516
LmnI GCTCC 4 cut(s) 340, 619, 1609, 1683
LweI GCATC 6 cut(s) 740, 842, 1169, 1336, 1588, 1985
MabI ACCWGGT 1 cut(s) 1453
MaeI CTAG 5 cut(s) 806, 1130, 1443, 1788, 1832
MaeII ACGT 4 cut(s) 875, 1489, 1774, 1848
MaeIII GTNAC 3 cut(s) 409, 777, 1339
MalI GATC 6 cut(s) 68, 670, 759, 1359, 1400, 1558
MboI GATC 6 cut(s) 66, 668, 757, 1357, 1398, 1556
MboII GAAGA 7 cut(s) 238, 448, 533, 933, 1267, 1306, 1809
MhlI GDGCHC 1 cut(s) 528
MlsI TGGCCA 1 cut(s) 1504
MluCI AATT 6 cut(s) 601, 936, 1378, 1752, 1812, 1915
MluI ACGCGT 1 cut(s) 57
MluNI TGGCCA 1 cut(s) 1504
MlyI GAGTC 2 cut(s) 269, 1574
MmeI TCCRAC 3 cut(s) 1090, 1436, 1631
Mox20I TGGCCA 1 cut(s) 1504
MroI TCCGGA 1 cut(s) 866
MroXI GAANNNNTTC 3 cut(s) 876, 971, 1378
MscI TGGCCA 1 cut(s) 1504
MseI TTAA 5 cut(s) 567, 849, 1572, 1938, 2004
MslI CAYNNNNRTG 1 cut(s) 1766
Msp20I TGGCCA 1 cut(s) 1504
MspA1I CMGCKG 2 cut(s) 326, 689
MspI CCGG 6 cut(s) 25, 39, 336, 867, 897, 1281
MspR9I CCNGG 3 cut(s) 337, 1281, 1455
Mva1269I GAATGC 3 cut(s) 260, 1051, 2029
MvaI CCWGG 1 cut(s) 1455
MvnI CGCG 3 cut(s) 59, 238, 1107
NciI CCSGG 2 cut(s) 337, 1281
NdeII GATC 6 cut(s) 66, 668, 757, 1357, 1398, 1556
NlaIII CATG 5 cut(s) 584, 686, 785, 1123, 1858
NlaIV GGNNCC 1 cut(s) 800
NmeAIII GCCGAG 2 cut(s) 513, 1974
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 2 cut(s) 686, 785
NspV TTCGAA 1 cut(s) 1799
PaeR7I CTCGAG 2 cut(s) 173, 365
PciI ACATGT 1 cut(s) 781
PciSI GCTCTTC 3 cut(s) 251, 431, 516
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 3 cut(s) 260, 1051, 2029
PdmI GAANNNNTTC 3 cut(s) 876, 971, 1378
PfeI GAWTC 2 cut(s) 1296, 1374
PfoI TCCNGGA 1 cut(s) 1279
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 2 cut(s) 268, 1573
PpsI GAGTC 2 cut(s) 268, 1573
PscI ACATGT 1 cut(s) 781
PshBI ATTAAT 1 cut(s) 849
PsiI TTATAA 1 cut(s) 552
Psp1406I AACGTT 2 cut(s) 875, 1848
Psp6I CCWGG 1 cut(s) 1453
PspFI CCCAGC 3 cut(s) 692, 1196, 1655
PspGI CCWGG 1 cut(s) 1453
PspN4I GGNNCC 1 cut(s) 800
PspPI GGNCC 3 cut(s) 799, 1635, 1874
PspXI VCTCGAGB 1 cut(s) 173
PstNI CAGNNNCTG 2 cut(s) 692, 946
PvuI CGATCG 1 cut(s) 69
RsaI GTAC 2 cut(s) 56, 320
RsaNI GTAC 2 cut(s) 55, 319
RseI CAYNNNNRTG 1 cut(s) 1766
SalI GTCGAC 1 cut(s) 1885
SapI GCTCTTC 3 cut(s) 251, 431, 516
SaqAI TTAA 5 cut(s) 567, 849, 1572, 1938, 2004
Sau3AI GATC 6 cut(s) 66, 668, 757, 1357, 1398, 1556
Sau96I GGNCC 3 cut(s) 799, 1635, 1874
SchI GAGTC 2 cut(s) 269, 1574
ScrFI CCNGG 3 cut(s) 337, 1281, 1455
SduI GDGCHC 1 cut(s) 528
SexAI ACCWGGT 1 cut(s) 1453
SfaNI GCATC 6 cut(s) 740, 842, 1169, 1336, 1588, 1985
Sfr274I CTCGAG 2 cut(s) 173, 365
SfuI TTCGAA 1 cut(s) 1799
SinI GGWCC 1 cut(s) 1874
SlaI CTCGAG 2 cut(s) 173, 365
SmiMI CAYNNNNRTG 1 cut(s) 1766
SmlI CTYRAG 2 cut(s) 173, 365
SmoI CTYRAG 2 cut(s) 173, 365
Sse9I AATT 6 cut(s) 601, 936, 1378, 1752, 1812, 1915
SspI AATATT 1 cut(s) 1206
SspMI CTAG 5 cut(s) 806, 1130, 1443, 1788, 1832
StyD4I CCNGG 3 cut(s) 335, 1279, 1453
StyI CCWWGG 1 cut(s) 763
TaaI ACNGT 4 cut(s) 676, 1768, 1878, 1959
TaiI ACGT 4 cut(s) 878, 1492, 1777, 1851
TaqII GACCGA 1 cut(s) 1773
TasI AATT 6 cut(s) 601, 936, 1378, 1752, 1812, 1915
TatI WGTACW 1 cut(s) 318
TauI GCSGC 6 cut(s) 83, 171, 587, 590, 692, 812
TfiI GAWTC 2 cut(s) 1296, 1374
Tru1I TTAA 5 cut(s) 567, 849, 1572, 1938, 2004
Tru9I TTAA 5 cut(s) 567, 849, 1572, 1938, 2004
TscAI CASTG 2 cut(s) 186, 760
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 4 cut(s) 569, 1371, 1526, 1782
TspGWI ACGGA 4 cut(s) 828, 1030, 1322, 1395
TspRI CASTG 2 cut(s) 186, 760
VpaK11BI GGWCC 1 cut(s) 1874
VspI ATTAAT 1 cut(s) 849
XapI RAATTY 3 cut(s) 601, 936, 1915
XbaI TCTAGA 1 cut(s) 1831
XceI RCATGY 2 cut(s) 686, 785
XhoI CTCGAG 2 cut(s) 173, 365
XmiI GTMKAC 2 cut(s) 1322, 1886
XmnI GAANNNNTTC 3 cut(s) 876, 971, 1378
XspI CTAG 5 cut(s) 806, 1130, 1443, 1788, 1832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.