Rroxscaffold_5G00372800

Ankyrin repeats (3 copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002201
Physical Location & Seq
Reverse (-)
54129772 .. 54133037
3266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372800.1

Sequence Viewer

Length: 1998 bp
ATGCCGCCGTCATACTTCCCTCTCCGGTGGGAAAGCACCGGAGACCAGTGGTGGTACGCGTCACCTATCGATTGGGCAGCAGCCAATGGCCTTTACGACCTTGTCAGCGAGCTTCTTCACCTCGACACCAACCTCCTCATCAAGCTGACATCGCTCCGCCGCATCCGCCGCCTCGAAACCGTGTGGGACGACGAGGCTCACTTCAATGACGTGGCGAAATGCCGCTCTCAAGTTGCCAAGAGGCTTCTCAATGAGTGCCAGACACAGAGTGGCCACAACTCTCTGATCCGGGCCGGCTACGGCGGCTGGCTCCTCTACACCGCCGCCTCAGCCGGTGACGTGGCCTTTGTTAAGGAGTTGCTAGCAAGAGACCCTCTTTTGGTGTTTGGGGAAGGAGAGTATGGGGTGACTGATATCTTTTATGCTGCTGCCAGGAGTAAGAATGCTGAGGTTTTCAGGCTGCTGCTAGATTTTTCGGTGTCGCCGAGGTTTGGTGAGGAGAATGGGGAGCTGGAGGAAAGGATGGTCGATAGTAGGTCGGATTTTAAGTGGGAGATGATGAACCGGGCGGTTCATGCGGCGGCGAGAGGTGGGAATCAGGAGGTTTTGAAGGAGTTGGTTGGGGATTGTGGTGATGTTTTGGTTTATAGGGATGCTCAGGGCTCTACTGTCTTGCATACTGCCTCTGGTAGAGGGCAGATTGAGGTGGTTAAATATCTAATAGCATCCTTTGATATCAGCACATTTGTAGATAGTCAAGGGAATACAGCTTTACATGTTGCCGCTTACAGGGGTCACTTAGCTGTGGTGGAAGTGCTAATTGGTGCATCTCCCTCATTAGTCTCTTTATCGAACTACTATGGAGATACATTTCTACACATGGCAGTGGCTGGTTTCCGAGCTCCTGGTTTCCGGAGAGTGGACAAACAGATTGAGCTCATCAAGCAATTAGTATGTGGTGATATTGTGAACATGCAGGACATCGTTAATGATAGGAACAACAATGGAAGAACAGCTCTTCACATAGCTGTTAGTGAGAACATACAGTCTAGTGTGGCGGAACTCCTCATGACTGTTCCGTCAATCGATCTGAACATCCGTGATGGTGATGGCATGACCCCATTGGATATTCTTAAGCAAAGGCCAAAGTCACCATCTTCTGAACTTTTGATCAAGCAGTTGATTTCAGCTGGAGGAATCTCCAAGTGTCGAGATCATAAAGCGAGAAATGCCCTTGTTTCTCATTTGAGAATGCATGGTATTGGGAGCAGTCCTGGAACCTCTTTCCGAATCCCTGATGCAGAGATATTCTTATACACGGGCAATGATAATGCTTCCGATGCCAGTGGTGATCAGTCATGTCTACAATTCAGTAGGTGCTCGGGTGAAATAAGTCAGGTTGATTCACCCAACTCAGTGAGCAATAAGAAGACTGGTTCTGTAAATTATGCTGCAAGGAGCCTTAAGTTTCTTCTTCAATGGCCGAGGAGGAAAGTAAAAAAGGAATCTAGCAGAGACTTGGGAGATGCTGATTCCTTGGACTCGTATAGTCCATATACAGATTTGGAAGACAGTCCAATCCCACTTCGGCAGATGTATTTGAAATCTTCATCCCTTCCCAACAGCAAAAGGATACTTTCTGCCAGGACTTATCTTCCAAGCCCCTACACTAAAATGAGATACACTGCTGGTCTAACACATGGTGTGATTCAAGCAGTGCCTCAGTTTGCTTTTCCAGCTCAATCACATGCACGTCCTCTGTCACGATCATTCATGTCTTCACCTGCCTTCGTGGAGAAGGAGAAGGGTGTTGACAATGTAGGATCGTCTCGCTTAATTGGGAAAGTATCGGTAGGGAATTCCAGACAAAATTCCTTCAATAGGAAGTTAATAAACCAATATTTGTGTGTTGGTGCACAAGGCGTGGAGGCTGAAGAATCAATTAGCTCTACGTGGCCAACTCCGAGCTACAAACATTCCAGTTCTTTAGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

665

Amino Acids

73.31

Weight (kDa)

7.63

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 189 - 246 1.6e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 192 - 277 1.2e-15 Ankyrin repeats (3 copies)
Ank_2 PF12796 250 - 296 7.4e-06 Ankyrin repeats (3 copies)
Ank PF00023 253 - 280 1.6e-06 Ankyrin repeat
Ank_5 PF13857 329 - 377 1.7e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1792
AasI GACNNNNNNGTC 1 cut(s) 1078
Acc36I ACCTGC 1 cut(s) 1792
AccBSI CCGCTC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 1363
AccII CGCG 1 cut(s) 59
AccIII TCCGGA 1 cut(s) 912
AclWI GGATC 2 cut(s) 280, 1831
AcoI YGGCCR 3 cut(s) 271, 1481, 1955
AcsI RAATTY 2 cut(s) 1858, 1870
AcuI CTGAAG 1 cut(s) 1953
AdeI CACNNNGTG 2 cut(s) 269, 1703
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 5 cut(s) 379, 491, 789, 919, 1881
AflII CTTAAG 2 cut(s) 1133, 1463
AflIII ACRYGT 2 cut(s) 57, 775
AgsI TTSAA 6 cut(s) 205, 610, 1478, 1603, 1712, 1879
AjiI CACGTC 3 cut(s) 211, 340, 1754
AjnI CCWGG 4 cut(s) 431, 904, 1273, 1643
AjuI GAANNNNNNNTTGG 2 cut(s) 804, 836
Alw21I GWGCWC 4 cut(s) 904, 939, 1382, 1918
Alw26I GTCTC 5 cut(s) 36, 363, 847, 1509, 1833
Alw44I GTGCAC 1 cut(s) 1914
AlwI GGATC 2 cut(s) 280, 1831
AlwNI CAGNNNCTG 1 cut(s) 890
Ama87I CYCGRG 1 cut(s) 1381
Aor13HI TCCGGA 1 cut(s) 912
AoxI GGCC 7 cut(s) 88, 271, 291, 342, 1142, 1481, 1955
ApaLI GTGCAC 1 cut(s) 1914
ApeKI GCWGC 7 cut(s) 77, 80, 425, 428, 460, 463, 1451
ApoI RAATTY 2 cut(s) 1858, 1870
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 2 cut(s) 290, 566
AsuNHI GCTAGC 1 cut(s) 361
AvaI CYCGRG 1 cut(s) 1381
BaeGI GKGCMC 1 cut(s) 1918
BalI TGGCCA 2 cut(s) 273, 1957
BanII GRGCYC 3 cut(s) 665, 904, 939
BbsI GAAGAC 3 cut(s) 1436, 1575, 1770
Bbv12I GWGCWC 4 cut(s) 904, 939, 1382, 1918
BbvCI CCTCAGC 2 cut(s) 328, 447
BbvI GCAGC 7 cut(s) 89, 92, 412, 415, 447, 450, 1438
BccI CCATC 4 cut(s) 517, 1097, 1103, 1162
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 4 cut(s) 433, 906, 1275, 1645
BciVI GTATCC 1 cut(s) 1626
BclI TGATCA 2 cut(s) 1170, 1351
BcnI CCSGG 2 cut(s) 290, 566
BcoDI GTCTC 5 cut(s) 36, 363, 847, 1509, 1833
BfaI CTAG 4 cut(s) 362, 467, 1050, 1509
BfrI CTTAAG 2 cut(s) 1133, 1463
BfuAI ACCTGC 1 cut(s) 1792
BfuI GTATCC 1 cut(s) 1626
Bme1390I CCNGG 6 cut(s) 290, 433, 566, 906, 1275, 1645
BmeT110I CYCGRG 1 cut(s) 1381
BmgBI CACGTC 3 cut(s) 211, 340, 1754
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 3 cut(s) 311, 1279, 1460
BmrFI CCNGG 6 cut(s) 290, 433, 566, 906, 1275, 1645
BmsI GCATC 7 cut(s) 171, 643, 734, 836, 1288, 1330, 1516
BmtI GCTAGC 1 cut(s) 365
BpiI GAAGAC 3 cut(s) 1436, 1575, 1770
BpmI CTGGAG 2 cut(s) 533, 1212
Bpu10I CCTNAGC 3 cut(s) 328, 447, 657
BpuEI CTTGAG 1 cut(s) 213
BpuMI CCSGG 2 cut(s) 290, 566
Bsa29I ATCGAT 2 cut(s) 69, 1086
BsaAI YACGTR 1 cut(s) 1953
BsaI GGTCTC 2 cut(s) 36, 363
BsaJI CCNNGG 3 cut(s) 485, 1484, 1536
BsaWI WCCGGW 3 cut(s) 24, 38, 912
Bsc4I CCNNNNNNNGG 5 cut(s) 379, 491, 789, 919, 1881
Bse118I RCCGGY 2 cut(s) 293, 332
Bse1I ACTGG 4 cut(s) 46, 1344, 1438, 1980
Bse3DI GCAATG 1 cut(s) 1330
BseAI TCCGGA 1 cut(s) 912
BseBI CCWGG 4 cut(s) 433, 906, 1275, 1645
BseCI ATCGAT 2 cut(s) 69, 1086
BseDI CCNNGG 3 cut(s) 485, 1484, 1536
BseGI GGATG 6 cut(s) 162, 528, 658, 725, 1095, 1610
BseLI CCNNNNNNNGG 5 cut(s) 379, 491, 789, 919, 1881
BseMI GCAATG 1 cut(s) 1330
BseMII CTCAG 5 cut(s) 342, 438, 671, 1428, 1736
BseNI ACTGG 4 cut(s) 46, 1344, 1438, 1980
BseRI GAGGAG 5 cut(s) 125, 302, 512, 1055, 1501
BseSI GKGCMC 1 cut(s) 1918
BseXI GCAGC 7 cut(s) 89, 92, 412, 415, 447, 450, 1438
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 7 cut(s) 90, 273, 293, 344, 1144, 1483, 1957
BshVI ATCGAT 2 cut(s) 69, 1086
BsiHKAI GWGCWC 4 cut(s) 904, 939, 1382, 1918
BsiHKCI CYCGRG 1 cut(s) 1381
BsiSI CCGG 7 cut(s) 25, 39, 289, 294, 333, 565, 913
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 5 cut(s) 379, 491, 789, 919, 1881
BsmAI GTCTC 5 cut(s) 36, 363, 847, 1509, 1833
BsmBI CGTCTC 1 cut(s) 1833
BsmFI GGGAC 1 cut(s) 200
BsmI GAATGC 2 cut(s) 448, 1257
BsnI GGCC 7 cut(s) 90, 273, 293, 344, 1144, 1483, 1957
Bso31I GGTCTC 2 cut(s) 36, 363
BsoBI CYCGRG 1 cut(s) 1381
Bsp1286I GDGCHC 5 cut(s) 665, 904, 939, 1382, 1918
Bsp13I TCCGGA 1 cut(s) 912
Bsp143I GATC 7 cut(s) 285, 1087, 1170, 1213, 1351, 1766, 1823
BspANI GGCC 7 cut(s) 90, 273, 293, 344, 1144, 1483, 1957
BspCNI CTCAG 5 cut(s) 341, 439, 670, 1427, 1735
BspDI ATCGAT 2 cut(s) 69, 1086
BspEI TCCGGA 1 cut(s) 912
BspFNI CGCG 1 cut(s) 59
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 3 cut(s) 311, 1279, 1460
BspMI ACCTGC 1 cut(s) 1792
BspOI GCTAGC 1 cut(s) 365
BspPI GGATC 2 cut(s) 280, 1831
BspQI GCTCTTC 1 cut(s) 1023
BspTI CTTAAG 2 cut(s) 1133, 1463
BspTNI GGTCTC 2 cut(s) 36, 363
BsrBI CCGCTC 1 cut(s) 225
BsrDI GCAATG 1 cut(s) 1330
BsrFI RCCGGY 2 cut(s) 293, 332
BsrI ACTGG 4 cut(s) 46, 1344, 1438, 1980
BssAI RCCGGY 2 cut(s) 293, 332
BssECI CCNNGG 3 cut(s) 485, 1484, 1536
BssMI GATC 7 cut(s) 285, 1087, 1170, 1213, 1351, 1766, 1823
BssT1I CCWWGG 1 cut(s) 1536
Bst2UI CCWGG 4 cut(s) 433, 906, 1275, 1645
Bst4CI ACNGT 5 cut(s) 181, 670, 1047, 1075, 1574
Bst6I CTCTTC 1 cut(s) 1023
BstAFI CTTAAG 2 cut(s) 1133, 1463
BstBAI YACGTR 1 cut(s) 1953
BstC8I GCNNGC 4 cut(s) 110, 295, 308, 363
BstDEI CTNAG 6 cut(s) 328, 447, 657, 799, 1414, 1722
BstENI CCTNNNNNAGG 1 cut(s) 1879
BstF5I GGATG 6 cut(s) 162, 528, 658, 725, 1095, 1610
BstFNI CGCG 1 cut(s) 59
BstKTI GATC 7 cut(s) 288, 1090, 1173, 1216, 1354, 1769, 1826
BstMAI GTCTC 5 cut(s) 36, 363, 847, 1509, 1833
BstMBI GATC 7 cut(s) 285, 1087, 1170, 1213, 1351, 1766, 1823
BstNI CCWGG 4 cut(s) 433, 906, 1275, 1645
BstNSI RCATGY 3 cut(s) 779, 976, 1751
BstSCI CCNGG 6 cut(s) 288, 431, 564, 904, 1273, 1643
BstSLI GKGCMC 1 cut(s) 1918
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 7 cut(s) 89, 92, 412, 415, 447, 450, 1438
BstV2I GAAGAC 3 cut(s) 1436, 1575, 1770
Bsu15I ATCGAT 2 cut(s) 69, 1086
BsuI GTATCC 1 cut(s) 1626
BsuRI GGCC 7 cut(s) 90, 273, 293, 344, 1144, 1483, 1957
BsuTUI ATCGAT 2 cut(s) 69, 1086
BtgZI GCGATG 1 cut(s) 135
BtrI CACGTC 3 cut(s) 211, 340, 1754
BtsCI GGATG 6 cut(s) 162, 528, 658, 725, 1095, 1610
BtsI GCAGTG 3 cut(s) 891, 1683, 1722
BtsIMutI CAGTG 6 cut(s) 53, 891, 1351, 1422, 1683, 1722
BveI ACCTGC 1 cut(s) 1792
Cac8I GCNNGC 4 cut(s) 110, 295, 308, 363
CaiI CAGNNNCTG 1 cut(s) 890
CciI TCATGA 1 cut(s) 1068
Cfr10I RCCGGY 2 cut(s) 293, 332
Cfr13I GGNCC 1 cut(s) 291
ClaI ATCGAT 2 cut(s) 69, 1086
CseI GACGC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 55
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 6 cut(s) 328, 447, 657, 799, 1414, 1722
DpnI GATC 7 cut(s) 287, 1089, 1172, 1215, 1353, 1768, 1825
DpnII GATC 7 cut(s) 285, 1087, 1170, 1213, 1351, 1766, 1823
DraIII CACNNNGTG 2 cut(s) 269, 1703
DrdI GACNNNNNNGTC 1 cut(s) 1078
DseDI GACNNNNNNGTC 1 cut(s) 1078
EaeI YGGCCR 3 cut(s) 271, 1481, 1955
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
EciI GGCGGA 3 cut(s) 146, 155, 1073
Ecl136II GAGCTC 2 cut(s) 902, 937
Eco130I CCWWGG 1 cut(s) 1536
Eco24I GRGCYC 3 cut(s) 665, 904, 939
Eco31I GGTCTC 2 cut(s) 36, 363
Eco32I GATATC 2 cut(s) 415, 736
Eco53kI GAGCTC 2 cut(s) 902, 937
Eco57I CTGAAG 1 cut(s) 1953
Eco88I CYCGRG 1 cut(s) 1381
EcoICRI GAGCTC 2 cut(s) 902, 937
EcoNI CCTNNNNNAGG 1 cut(s) 1879
EcoRI GAATTC 1 cut(s) 1858
EcoRII CCWGG 4 cut(s) 431, 904, 1273, 1643
EcoRV GATATC 2 cut(s) 415, 736
EcoT14I CCWWGG 1 cut(s) 1536
EcoT22I ATGCAT 1 cut(s) 1257
EcoT38I GRGCYC 3 cut(s) 665, 904, 939
ErhI CCWWGG 1 cut(s) 1536
Esp3I CGTCTC 1 cut(s) 1833
FaqI GGGAC 1 cut(s) 200
FbaI TGATCA 2 cut(s) 1170, 1351
FblI GTMKAC 1 cut(s) 1363
FokI GGATG 6 cut(s) 149, 535, 665, 712, 1082, 1597
FriOI GRGCYC 3 cut(s) 665, 904, 939
FspBI CTAG 4 cut(s) 362, 467, 1050, 1509
GsuI CTGGAG 2 cut(s) 533, 1212
HaeIII GGCC 7 cut(s) 90, 273, 293, 344, 1144, 1483, 1957
HapII CCGG 7 cut(s) 25, 39, 289, 294, 333, 565, 913
HgaI GACGC 1 cut(s) 48
HincII GTYRAC 1 cut(s) 1813
HindII GTYRAC 1 cut(s) 1813
HinfI GANTC 9 cut(s) 595, 1197, 1290, 1403, 1505, 1532, 1541, 1708, 1937
HpaII CCGG 7 cut(s) 25, 39, 289, 294, 333, 565, 913
Hpy166II GTNNAC 5 cut(s) 922, 970, 1364, 1813, 1916
Hpy188I TCNGA 8 cut(s) 285, 541, 899, 1092, 1162, 1289, 1339, 1965
Hpy188III TCNNGA 6 cut(s) 599, 913, 1069, 1211, 1764, 1863
Hpy8I GTNNAC 5 cut(s) 922, 970, 1364, 1813, 1916
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 7 cut(s) 386, 604, 1625, 1792, 1798, 1798, 1885
HpyCH4III ACNGT 5 cut(s) 181, 670, 1047, 1075, 1574
HpyCH4IV ACGT 4 cut(s) 210, 339, 1753, 1952
HpyCH4V TGCA 8 cut(s) 676, 827, 976, 1255, 1301, 1454, 1751, 1916
HpyF3I CTNAG 6 cut(s) 328, 447, 657, 799, 1414, 1722
HpySE526I ACGT 4 cut(s) 210, 339, 1753, 1952
Kpn2I TCCGGA 1 cut(s) 912
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Ksp22I TGATCA 2 cut(s) 1170, 1351
Kzo9I GATC 7 cut(s) 285, 1087, 1170, 1213, 1351, 1766, 1823
LguI GCTCTTC 1 cut(s) 1023
LmnI GCTCC 6 cut(s) 159, 315, 508, 907, 1266, 1458
Lsp1109I GCAGC 7 cut(s) 89, 92, 412, 415, 447, 450, 1438
LweI GCATC 7 cut(s) 171, 643, 734, 836, 1288, 1330, 1516
MaeI CTAG 4 cut(s) 362, 467, 1050, 1509
MaeII ACGT 4 cut(s) 210, 339, 1753, 1952
MaeIII GTNAC 6 cut(s) 60, 335, 406, 794, 1149, 1761
MalI GATC 7 cut(s) 287, 1089, 1172, 1215, 1353, 1768, 1825
MbiI CCGCTC 1 cut(s) 225
MboI GATC 7 cut(s) 285, 1087, 1170, 1213, 1351, 1766, 1823
MhlI GDGCHC 5 cut(s) 665, 904, 939, 1382, 1918
MlsI TGGCCA 2 cut(s) 273, 1957
MluCI AATT 8 cut(s) 819, 947, 1367, 1444, 1836, 1858, 1870, 1941
MluI ACGCGT 1 cut(s) 57
MluNI TGGCCA 2 cut(s) 273, 1957
MlyI GAGTC 1 cut(s) 1535
MmeI TCCRAC 1 cut(s) 519
Mox20I TGGCCA 2 cut(s) 273, 1957
Mph1103I ATGCAT 1 cut(s) 1257
MroI TCCGGA 1 cut(s) 912
MroNI GCCGGC 1 cut(s) 293
MscI TGGCCA 2 cut(s) 273, 1957
MseI TTAA 8 cut(s) 351, 546, 711, 987, 1134, 1464, 1835, 1889
MslI CAYNNNNRTG 3 cut(s) 204, 884, 1673
Msp20I TGGCCA 2 cut(s) 273, 1957
MspA1I CMGCKG 1 cut(s) 1190
MspCI CTTAAG 2 cut(s) 1133, 1463
MspI CCGG 7 cut(s) 25, 39, 289, 294, 333, 565, 913
MspR9I CCNGG 6 cut(s) 290, 433, 566, 906, 1275, 1645
Mva1269I GAATGC 2 cut(s) 448, 1257
MvaI CCWGG 4 cut(s) 433, 906, 1275, 1645
MvnI CGCG 1 cut(s) 59
NaeI GCCGGC 1 cut(s) 295
NciI CCSGG 2 cut(s) 290, 566
NdeII GATC 7 cut(s) 285, 1087, 1170, 1213, 1351, 1766, 1823
NgoMIV GCCGGC 1 cut(s) 293
NheI GCTAGC 1 cut(s) 361
NlaIV GGNNCC 3 cut(s) 311, 1279, 1460
NmeAIII GCCGAG 2 cut(s) 510, 1509
NmuCI GTSAC 6 cut(s) 60, 335, 406, 794, 1149, 1761
NsiI ATGCAT 1 cut(s) 1257
NspI RCATGY 3 cut(s) 779, 976, 1751
PagI TCATGA 1 cut(s) 1068
PaqCI CACCTGC 1 cut(s) 1792
PciI ACATGT 1 cut(s) 775
PciSI GCTCTTC 1 cut(s) 1023
PcsI WCGNNNNNNNCGW 1 cut(s) 482
PctI GAATGC 2 cut(s) 448, 1257
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 8 cut(s) 595, 1197, 1290, 1403, 1505, 1532, 1708, 1937
PflFI GACNNNGTC 1 cut(s) 101
PfoI TCCNGGA 1 cut(s) 1273
PleI GAGTC 1 cut(s) 1535
PpsI GAGTC 1 cut(s) 1535
Ppu21I YACGTR 1 cut(s) 1953
PscI ACATGT 1 cut(s) 775
Psp124BI GAGCTC 2 cut(s) 904, 939
Psp6I CCWGG 4 cut(s) 431, 904, 1273, 1643
PspGI CCWGG 4 cut(s) 431, 904, 1273, 1643
PspN4I GGNNCC 3 cut(s) 311, 1279, 1460
PspPI GGNCC 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 890
PsyI GACNNNGTC 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 1190
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 3 cut(s) 204, 884, 1673
SacI GAGCTC 2 cut(s) 904, 939
SapI GCTCTTC 1 cut(s) 1023
SaqAI TTAA 8 cut(s) 351, 546, 711, 987, 1134, 1464, 1835, 1889
Sau3AI GATC 7 cut(s) 285, 1087, 1170, 1213, 1351, 1766, 1823
Sau96I GGNCC 1 cut(s) 291
SchI GAGTC 1 cut(s) 1535
ScrFI CCNGG 6 cut(s) 290, 433, 566, 906, 1275, 1645
SduI GDGCHC 5 cut(s) 665, 904, 939, 1382, 1918
SfaNI GCATC 7 cut(s) 171, 643, 734, 836, 1288, 1330, 1516
SmiMI CAYNNNNRTG 3 cut(s) 204, 884, 1673
SmlI CTYRAG 3 cut(s) 228, 1133, 1463
SmoI CTYRAG 3 cut(s) 228, 1133, 1463
Sse9I AATT 8 cut(s) 819, 947, 1367, 1444, 1836, 1858, 1870, 1941
SspI AATATT 1 cut(s) 1901
SspMI CTAG 4 cut(s) 362, 467, 1050, 1509
SstI GAGCTC 2 cut(s) 904, 939
StyD4I CCNGG 6 cut(s) 288, 431, 564, 904, 1273, 1643
StyI CCWWGG 1 cut(s) 1536
TaaI ACNGT 5 cut(s) 181, 670, 1047, 1075, 1574
TaiI ACGT 4 cut(s) 213, 342, 1756, 1955
TaqI TCGA 7 cut(s) 69, 123, 174, 528, 851, 1086, 1210
TasI AATT 8 cut(s) 819, 947, 1367, 1444, 1836, 1858, 1870, 1941
TauI GCSGC 9 cut(s) 7, 162, 171, 225, 306, 326, 581, 584, 785
TfiI GAWTC 8 cut(s) 595, 1197, 1290, 1403, 1505, 1532, 1708, 1937
Tru1I TTAA 8 cut(s) 351, 546, 711, 987, 1134, 1464, 1835, 1889
Tru9I TTAA 8 cut(s) 351, 546, 711, 987, 1134, 1464, 1835, 1889
TscAI CASTG 6 cut(s) 53, 891, 1351, 1422, 1690, 1722
TseFI GTSAC 6 cut(s) 60, 335, 406, 794, 1149, 1761
TseI GCWGC 7 cut(s) 77, 80, 425, 428, 460, 463, 1451
Tsp45I GTSAC 6 cut(s) 60, 335, 406, 794, 1149, 1761
TspDTI ATGAA 4 cut(s) 563, 575, 1599, 1762
TspGWI ACGGA 2 cut(s) 1068, 1088
TspRI CASTG 6 cut(s) 53, 891, 1351, 1422, 1690, 1722
Tth111I GACNNNGTC 1 cut(s) 101
Vha464I CTTAAG 2 cut(s) 1133, 1463
VneI GTGCAC 1 cut(s) 1914
XagI CCTNNNNNAGG 1 cut(s) 1879
XapI RAATTY 2 cut(s) 1858, 1870
XceI RCATGY 3 cut(s) 779, 976, 1751
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 1363
XspI CTAG 4 cut(s) 362, 467, 1050, 1509
Zsp2I ATGCAT 1 cut(s) 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.