FvH4_4g29580

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003407
Physical Location & Seq
Reverse (-)
29654931 .. 29656612
1682 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29580.t1

Sequence Viewer

Length: 714 bp
ATGGGGAGGATAGGCACAAAGTTGCCAAGTTTCTGCCTCAACAGGATTAGGCCTCATGTTCGAGTTCGTTCTCCACCGATACAGTCGAAACTAGATGCTGCAGAGATCACTAAAAATGGTGGGAAAGTTGAGAATTGTGGCAATGCTGGAGAAGAGAAAGTTACAGATGGAGATGGGGCTAAAAAATCCGGGGTGGTTTTTGCGAGGAAGATCATGATTGTGGTTGATTCGAGCATTGAAGCCAAGGGAGCTCTGCAATGGGCTCTTTCTCACACTGTTCAGAACCAGGACAAGCTTGTACTGCTACATGTAACCAAACCTTCAACTTCAAAACAAGGTGAGGATATTATAGCTCCAAGGGCTTATGAACTTGTTCATTCTCTGAGGAATATGTGCCAGCTGAAGAGACCTGAGGTACAAACTGAGGTTGCAGTGGTGGAATTAGGGAAAAAGGAGAAAGGGCCAACAATAGTGGAAGAGGCCAAAAAGCAAGGGGTGTCACTGCTGGTTCTGGGACAGAAGAAGAGGTCTACGACGTGGCGGCTGCTGATGATGTGGGCGGGGAGCAACCGTGTCGGTGGCGGGGGCGGGGTGGTGGAGTACTGTATACAGAATGCCAGTTGCATGGCGATTGCAGTGAGGAGGAAAAGCAAGAAGGTTGGAGGGTACTTGATCACTACAAAGCGCCAGAAAGATTTCTGGCTCTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

238

Amino Acids

25.96

Weight (kDa)

9.96

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 69 - 214 8.5e-18 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 530, 607
AciI CCGC 4 cut(s) 541, 560, 582, 588
AcuI CTGAAG 1 cut(s) 422
AfaI GTAC 4 cut(s) 300, 417, 602, 668
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 3 cut(s) 239, 324, 330
AjiI CACGTC 1 cut(s) 537
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 4 cut(s) 251, 295, 353, 400
AluI AGCT 4 cut(s) 251, 295, 353, 400
Alw21I GWGCWC 1 cut(s) 253
Alw26I GTCTC 1 cut(s) 400
AoxI GGCC 4 cut(s) 50, 461, 480, 708
ApeKI GCWGC 2 cut(s) 98, 544
Asp700I GAANNNNTTC 2 cut(s) 372, 695
AspLEI GCGC 1 cut(s) 687
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 350
AxyI CCTNAGG 1 cut(s) 411
BanII GRGCYC 2 cut(s) 253, 265
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 2 cut(s) 85, 531
BccI CCATC 2 cut(s) 161, 167
BcgI CGANNNNNNTGC 2 cut(s) 556, 590
BciT130I CCWGG 1 cut(s) 287
BclI TGATCA 1 cut(s) 672
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 2 cut(s) 92, 712
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 688
BisI GCNGC 3 cut(s) 99, 542, 545
BlsI GCNGC 3 cut(s) 100, 543, 546
BmcAI AGTACT 1 cut(s) 602
Bme1390I CCNGG 2 cut(s) 190, 287
BmgBI CACGTC 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 461
BmrFI CCNGG 2 cut(s) 190, 287
BmsI GCATC 1 cut(s) 85
BpmI CTGGAG 1 cut(s) 168
BpuMI CCSGG 1 cut(s) 190
BsaI GGTCTC 1 cut(s) 400
BsaJI CCNNGG 3 cut(s) 189, 243, 356
BsaXI ACNNNNNCTCC 2 cut(s) 556, 586
Bse1I ACTGG 1 cut(s) 618
Bse21I CCTNAGG 1 cut(s) 411
Bse3DI GCAATG 2 cut(s) 148, 263
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 3 cut(s) 189, 243, 356
BseMI GCAATG 2 cut(s) 148, 263
BseMII CTCAG 3 cut(s) 374, 402, 414
BseNI ACTGG 1 cut(s) 618
BseRI GAGGAG 1 cut(s) 655
BseXI GCAGC 2 cut(s) 85, 531
BshFI GGCC 4 cut(s) 52, 463, 482, 710
BsiHKAI GWGCWC 1 cut(s) 253
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 528
BsmAI GTCTC 1 cut(s) 400
BsmFI GGGAC 1 cut(s) 528
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 4 cut(s) 52, 463, 482, 710
Bso31I GGTCTC 1 cut(s) 400
Bsp1286I GDGCHC 2 cut(s) 253, 265
Bsp143I GATC 3 cut(s) 105, 210, 672
BspACI CCGC 4 cut(s) 541, 560, 582, 588
BspANI GGCC 4 cut(s) 52, 463, 482, 710
BspCNI CTCAG 3 cut(s) 375, 403, 415
BspHI TCATGA 1 cut(s) 213
BspMAI CTGCAG 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 400
BsrDI GCAATG 2 cut(s) 148, 263
BsrI ACTGG 1 cut(s) 618
BssECI CCNNGG 3 cut(s) 189, 243, 356
BssMI GATC 3 cut(s) 105, 210, 672
BssNAI GTATAC 1 cut(s) 608
BssT1I CCWWGG 2 cut(s) 243, 356
Bst1107I GTATAC 1 cut(s) 608
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 4 cut(s) 84, 277, 572, 605
Bst6I CTCTTC 4 cut(s) 147, 398, 471, 518
BstC8I GCNNGC 1 cut(s) 398
BstDEI CTNAG 3 cut(s) 383, 411, 423
BstH2I RGCGCY 1 cut(s) 688
BstHHI GCGC 1 cut(s) 687
BstKTI GATC 3 cut(s) 108, 213, 675
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 3 cut(s) 105, 210, 672
BstMWI GCNNNNNNNGC 3 cut(s) 248, 301, 359
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 188, 285
BstSFI CTRYAG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 85, 531
BstXI CCANNNNNNTGG 1 cut(s) 625
BstZ17I GTATAC 1 cut(s) 608
Bsu36I CCTNAGG 1 cut(s) 411
BsuRI GGCC 4 cut(s) 52, 463, 482, 710
BtrI CACGTC 1 cut(s) 537
BtsI GCAGTG 3 cut(s) 438, 500, 642
BtsIMutI CAGTG 4 cut(s) 273, 438, 500, 642
Cac8I GCNNGC 1 cut(s) 398
CciI TCATGA 1 cut(s) 213
CfoI GCGC 1 cut(s) 687
Cfr13I GGNCC 1 cut(s) 461
Csp6I GTAC 4 cut(s) 299, 416, 601, 667
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 4 cut(s) 56, 214, 308, 625
CviQI GTAC 4 cut(s) 299, 416, 601, 667
DdeI CTNAG 3 cut(s) 383, 411, 423
DpnI GATC 3 cut(s) 107, 212, 674
DpnII GATC 3 cut(s) 105, 210, 672
Eam1104I CTCTTC 4 cut(s) 147, 398, 471, 518
EarI CTCTTC 4 cut(s) 147, 398, 471, 518
Ecl136II GAGCTC 1 cut(s) 251
Eco130I CCWWGG 2 cut(s) 243, 356
Eco147I AGGCCT 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 253, 265
Eco31I GGTCTC 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 422
Eco81I CCTNAGG 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 2 cut(s) 243, 356
EcoT38I GRGCYC 2 cut(s) 253, 265
ErhI CCWWGG 2 cut(s) 243, 356
FaeI CATG 4 cut(s) 59, 217, 311, 628
FaiI YATR 8 cut(s) 57, 215, 309, 350, 366, 392, 608, 626
FaqI GGGAC 1 cut(s) 528
FatI CATG 4 cut(s) 55, 213, 307, 624
FauI CCCGC 3 cut(s) 553, 575, 581
FbaI TGATCA 1 cut(s) 672
FblI GTMKAC 2 cut(s) 530, 607
Fnu4HI GCNGC 3 cut(s) 99, 542, 545
FriOI GRGCYC 2 cut(s) 253, 265
Fsp4HI GCNGC 3 cut(s) 99, 542, 545
FspBI CTAG 2 cut(s) 92, 712
GlaI GCGC 1 cut(s) 686
GluI GCNGC 3 cut(s) 99, 542, 545
GsuI CTGGAG 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 688
HaeIII GGCC 4 cut(s) 52, 463, 482, 710
HapII CCGG 1 cut(s) 189
HhaI GCGC 1 cut(s) 687
Hin1II CATG 4 cut(s) 59, 217, 311, 628
Hin6I GCGC 1 cut(s) 685
HinP1I GCGC 1 cut(s) 685
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 1 cut(s) 227
HpaII CCGG 1 cut(s) 189
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 2 cut(s) 531, 608
Hpy188I TCNGA 2 cut(s) 282, 384
Hpy188III TCNNGA 1 cut(s) 214
Hpy8I GTNNAC 2 cut(s) 531, 608
Hpy99I CGWCG 1 cut(s) 538
HpyAV CCTTC 2 cut(s) 330, 649
HpyCH4III ACNGT 4 cut(s) 84, 277, 572, 605
HpyCH4IV ACGT 1 cut(s) 536
HpyCH4V TGCA 5 cut(s) 101, 256, 431, 624, 635
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 301, 359
HpyF3I CTNAG 3 cut(s) 383, 411, 423
HpySE526I ACGT 1 cut(s) 536
Hsp92II CATG 4 cut(s) 59, 217, 311, 628
HspAI GCGC 1 cut(s) 685
Ksp22I TGATCA 1 cut(s) 672
Kzo9I GATC 3 cut(s) 105, 210, 672
LmnI GCTCC 3 cut(s) 248, 358, 564
Lsp1109I GCAGC 2 cut(s) 85, 531
LweI GCATC 1 cut(s) 85
MaeI CTAG 2 cut(s) 92, 712
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 3 cut(s) 160, 310, 498
MalI GATC 3 cut(s) 107, 212, 674
MboI GATC 3 cut(s) 105, 210, 672
MboII GAAGA 6 cut(s) 164, 220, 415, 488, 532, 535
MhlI GDGCHC 2 cut(s) 253, 265
MluCI AATT 2 cut(s) 133, 440
MmeI TCCRAC 1 cut(s) 640
MroXI GAANNNNTTC 2 cut(s) 372, 695
MslI CAYNNNNRTG 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 400
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 2 cut(s) 190, 287
Mva1269I GAATGC 1 cut(s) 619
MvaI CCWGG 1 cut(s) 287
MwoI GCNNNNNNNGC 3 cut(s) 248, 301, 359
NciI CCSGG 1 cut(s) 190
NdeII GATC 3 cut(s) 105, 210, 672
NlaIII CATG 4 cut(s) 59, 217, 311, 628
NmuCI GTSAC 1 cut(s) 498
NspI RCATGY 1 cut(s) 311
PagI TCATGA 1 cut(s) 213
PceI AGGCCT 1 cut(s) 52
PciI ACATGT 1 cut(s) 307
PctI GAATGC 1 cut(s) 619
PdmI GAANNNNTTC 2 cut(s) 372, 695
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 3 cut(s) 100, 543, 546
PscI ACATGT 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspPI GGNCC 1 cut(s) 461
PstI CTGCAG 1 cut(s) 103
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 4 cut(s) 300, 417, 602, 668
RsaNI GTAC 4 cut(s) 299, 416, 601, 667
RseI CAYNNNNRTG 1 cut(s) 218
SacI GAGCTC 1 cut(s) 253
SatI GCNGC 3 cut(s) 99, 542, 545
Sau3AI GATC 3 cut(s) 105, 210, 672
Sau96I GGNCC 1 cut(s) 461
ScaI AGTACT 1 cut(s) 602
ScrFI CCNGG 2 cut(s) 190, 287
SduI GDGCHC 2 cut(s) 253, 265
SfaNI GCATC 1 cut(s) 85
SfcI CTRYAG 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 218
Sse9I AATT 2 cut(s) 133, 440
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 4 cut(s) 541, 560, 582, 588
SspMI CTAG 2 cut(s) 92, 712
SstI GAGCTC 1 cut(s) 253
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 2 cut(s) 188, 285
StyI CCWWGG 2 cut(s) 243, 356
TaaI ACNGT 4 cut(s) 84, 277, 572, 605
TaiI ACGT 1 cut(s) 539
TaqI TCGA 3 cut(s) 61, 86, 230
TasI AATT 2 cut(s) 133, 440
TatI WGTACW 2 cut(s) 298, 600
TauI GCSGC 1 cut(s) 544
TfiI GAWTC 1 cut(s) 227
TscAI CASTG 4 cut(s) 280, 438, 507, 642
TseFI GTSAC 1 cut(s) 498
TseI GCWGC 2 cut(s) 98, 544
Tsp45I GTSAC 1 cut(s) 498
TspDTI ATGAA 2 cut(s) 365, 381
TspRI CASTG 4 cut(s) 280, 438, 507, 642
XceI RCATGY 1 cut(s) 311
XmiI GTMKAC 2 cut(s) 530, 607
XmnI GAANNNNTTC 2 cut(s) 372, 695
XspI CTAG 2 cut(s) 92, 712
ZrmI AGTACT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.