MD14G1215800.v1.1

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003407
Physical Location & Seq
Forward (+)
29962211 .. 29963532
1322 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1215800.v1.1.491

Sequence Viewer

Length: 711 bp
ATGAGCAGGAAATATGCAAGGTTGTGTGGTGCCCGGCCGACTGTTTGTCTTCCAATCCATCCACTGTCTCTCCGGTCGAAACACACGTCTAGACGGCGTTCCGGTTCTGTCAAAGCTACTACTGATCATAACAGCACAGAGTTTTTGAACAATATCAGCAGCAGCGGGGATGATGACTTGAGTAGTACTGCAGAAGATGTGAAAAGTGGGAACAAGATTATGGTTGTTGTGGATTCAAGCCTTGAAGCTAAGGGAGCTCTCGAATGGGCACTATCCCACACTGTCCAATCCCAGGATACCATTGTTCTTCTTCATGTAGCTAATCCCTCCAAACAACAAGACTCAAATGGGAAGACTAATTTGAAGGCTTTTGAACTGCTTCACTCTATTAAAAATGTTTGTCAAAGAAAACGGCCTGGGGTGAATGTGGAGGTAGCATTGCTTGAAGGGAAAGAGAAGGGCCCAATAATAGTGGATGAAGCAAGGAGACAAAGAGTGTCCCTACTTGTTCTTGGCCAAAAAAGAAAGTGGTCGAACTGGTGGCAGCTTATCAAGAGGTGGACGAGGAGCAGATCATCGGGGGAGGTTTTTGTGGAATACTGCATTCAAAATGCTGCATGCATGACGATTGCAGTGAGGAGAAAGAGCAAGAAATTGGGAGGTTACTTGATCACCACCAAGCTTCACAAGAATTTCTGGCTTCTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

237

Amino Acids

26.49

Weight (kDa)

10.08

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 72 - 213 7.1e-14 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 29
AciI CCGC 1 cut(s) 165
AcoI YGGCCR 2 cut(s) 35, 514
AcsI RAATTY 1 cut(s) 691
AfaI GTAC 1 cut(s) 187
AfiI CCNNNNNNNGG 1 cut(s) 292
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 7 cut(s) 148, 237, 245, 364, 374, 446, 608
AhdI GACNNNNNGTC 1 cut(s) 45
AjiI CACGTC 1 cut(s) 87
AjnI CCWGG 2 cut(s) 291, 415
AluBI AGCT 6 cut(s) 116, 248, 257, 320, 547, 682
AluI AGCT 6 cut(s) 116, 248, 257, 320, 547, 682
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 2 cut(s) 72, 481
AoxI GGCC 4 cut(s) 35, 413, 460, 514
ApaI GGGCCC 1 cut(s) 464
ApeKI GCWGC 4 cut(s) 159, 162, 544, 614
ApoI RAATTY 1 cut(s) 691
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
Asp700I GAANNNNTTC 1 cut(s) 378
AspS9I GGNCC 2 cut(s) 460, 461
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 2 cut(s) 433, 664
BaeGI GKGCMC 3 cut(s) 34, 271, 464
BaeI ACNNNNGTAYC 2 cut(s) 288, 321
BalI TGGCCA 1 cut(s) 516
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 2 cut(s) 259, 464
BbsI GAAGAC 2 cut(s) 41, 359
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 4 cut(s) 171, 174, 556, 601
BccI CCATC 1 cut(s) 66
BceAI ACGGC 2 cut(s) 110, 428
BciT130I CCWGG 2 cut(s) 293, 417
BciVI GTATCC 1 cut(s) 289
BclI TGATCA 2 cut(s) 124, 669
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 2 cut(s) 72, 481
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 189
BfuI GTATCC 1 cut(s) 289
BisI GCNGC 4 cut(s) 160, 163, 545, 615
BlsI GCNGC 4 cut(s) 161, 164, 546, 616
BmcAI AGTACT 1 cut(s) 187
Bme1390I CCNGG 3 cut(s) 34, 293, 417
BmeRI GACNNNNNGTC 1 cut(s) 45
BmgBI CACGTC 1 cut(s) 87
BmgT120I GGNCC 2 cut(s) 460, 461
BmiI GGNNCC 2 cut(s) 31, 462
BmrFI CCNGG 3 cut(s) 34, 293, 417
BpiI GAAGAC 2 cut(s) 41, 359
Bpu10I CCTNAGC 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 199
BpuMI CCSGG 1 cut(s) 34
BsaJI CCNNGG 2 cut(s) 291, 416
BsaWI WCCGGW 2 cut(s) 72, 101
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 542
Bse3DI GCAATG 1 cut(s) 437
BseBI CCWGG 2 cut(s) 293, 417
BseDI CCNNGG 2 cut(s) 291, 416
BseGI GGATG 3 cut(s) 58, 175, 481
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMI GCAATG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 542
BseRI GAGGAG 2 cut(s) 580, 652
BseSI GKGCMC 3 cut(s) 34, 271, 464
BseX3I CGGCCG 1 cut(s) 35
BseXI GCAGC 4 cut(s) 171, 174, 556, 601
Bsh1285I CGRYCG 2 cut(s) 38, 77
BshFI GGCC 4 cut(s) 37, 415, 462, 516
BshNI GGYRCC 1 cut(s) 29
BsiEI CGRYCG 2 cut(s) 38, 77
BsiHKAI GWGCWC 1 cut(s) 259
BsiSI CCGG 3 cut(s) 34, 73, 102
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 1 cut(s) 292
BsmAI GTCTC 2 cut(s) 72, 481
BsmFI GGGAC 1 cut(s) 484
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 4 cut(s) 37, 415, 462, 516
Bsp120I GGGCCC 1 cut(s) 460
Bsp1286I GDGCHC 4 cut(s) 34, 259, 271, 464
Bsp143I GATC 3 cut(s) 124, 572, 669
BspACI CCGC 1 cut(s) 165
BspANI GGCC 4 cut(s) 37, 415, 462, 516
BspLI GGNNCC 2 cut(s) 31, 462
BspMAI CTGCAG 1 cut(s) 193
BspT107I GGYRCC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 437
BsrI ACTGG 1 cut(s) 542
BssECI CCNNGG 2 cut(s) 291, 416
BssMI GATC 3 cut(s) 124, 572, 669
Bst2UI CCWGG 2 cut(s) 293, 417
Bst4CI ACNGT 3 cut(s) 43, 66, 283
BstC8I GCNNGC 1 cut(s) 619
BstDEI CTNAG 1 cut(s) 249
BstF5I GGATG 3 cut(s) 58, 175, 481
BstKTI GATC 3 cut(s) 127, 575, 672
BstMAI GTCTC 2 cut(s) 72, 481
BstMBI GATC 3 cut(s) 124, 572, 669
BstMCI CGRYCG 2 cut(s) 38, 77
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 2 cut(s) 293, 417
BstNSI RCATGY 1 cut(s) 621
BstSCI CCNGG 3 cut(s) 32, 291, 415
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 3 cut(s) 34, 271, 464
BstV1I GCAGC 4 cut(s) 171, 174, 556, 601
BstV2I GAAGAC 2 cut(s) 41, 359
BstZI CGGCCG 1 cut(s) 35
BsuI GTATCC 1 cut(s) 289
BsuRI GGCC 4 cut(s) 37, 415, 462, 516
BtrI CACGTC 1 cut(s) 87
BtsCI GGATG 3 cut(s) 58, 175, 481
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 3 cut(s) 62, 279, 639
Cac8I GCNNGC 1 cut(s) 619
Cfr13I GGNCC 2 cut(s) 460, 461
Csp6I GTAC 1 cut(s) 186
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 3 cut(s) 314, 618, 622
CviQI GTAC 1 cut(s) 186
DdeI CTNAG 1 cut(s) 249
DpnI GATC 3 cut(s) 126, 574, 671
DpnII GATC 3 cut(s) 124, 572, 669
DriI GACNNNNNGTC 1 cut(s) 45
EaeI YGGCCR 2 cut(s) 35, 514
EagI CGGCCG 1 cut(s) 35
Eam1105I GACNNNNNGTC 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 257
EclXI CGGCCG 1 cut(s) 35
Eco24I GRGCYC 2 cut(s) 259, 464
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 460
EcoRII CCWGG 2 cut(s) 291, 415
EcoT22I ATGCAT 1 cut(s) 623
EcoT38I GRGCYC 2 cut(s) 259, 464
FaeI CATG 3 cut(s) 317, 621, 625
FaiI YATR 6 cut(s) 15, 129, 221, 315, 619, 623
FaqI GGGAC 1 cut(s) 484
FatI CATG 3 cut(s) 313, 617, 621
FauI CCCGC 1 cut(s) 158
FbaI TGATCA 2 cut(s) 124, 669
Fnu4HI GCNGC 4 cut(s) 160, 163, 545, 615
FokI GGATG 3 cut(s) 45, 182, 488
FriOI GRGCYC 2 cut(s) 259, 464
Fsp4HI GCNGC 4 cut(s) 160, 163, 545, 615
FspBI CTAG 1 cut(s) 90
GluI GCNGC 4 cut(s) 160, 163, 545, 615
HaeIII GGCC 4 cut(s) 37, 415, 462, 516
HapII CCGG 3 cut(s) 34, 73, 102
Hin1II CATG 3 cut(s) 317, 621, 625
HindIII AAGCTT 1 cut(s) 680
HinfI GANTC 2 cut(s) 233, 341
HpaII CCGG 3 cut(s) 34, 73, 102
HphI GGTGA 2 cut(s) 433, 664
Hpy166II GTNNAC 1 cut(s) 561
Hpy188III TCNNGA 3 cut(s) 90, 260, 553
Hpy8I GTNNAC 1 cut(s) 561
HpyAV CCTTC 3 cut(s) 358, 440, 451
HpyCH4III ACNGT 3 cut(s) 43, 66, 283
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 6 cut(s) 17, 191, 603, 617, 621, 632
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 1 cut(s) 249
HpySE526I ACGT 1 cut(s) 86
Hsp92II CATG 3 cut(s) 317, 621, 625
Ksp22I TGATCA 2 cut(s) 124, 669
Kzo9I GATC 3 cut(s) 124, 572, 669
LmnI GCTCC 2 cut(s) 254, 567
Lsp1109I GCAGC 4 cut(s) 171, 174, 556, 601
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 1 cut(s) 662
MalI GATC 3 cut(s) 126, 574, 671
MboI GATC 3 cut(s) 124, 572, 669
MboII GAAGA 5 cut(s) 41, 206, 299, 302, 364
MhlI GDGCHC 4 cut(s) 34, 259, 271, 464
MlsI TGGCCA 1 cut(s) 516
MluCI AATT 3 cut(s) 358, 653, 691
MluNI TGGCCA 1 cut(s) 516
MlyI GAGTC 1 cut(s) 335
MnlI CCTC 7 cut(s) 337, 424, 549, 558, 577, 630, 653
Mox20I TGGCCA 1 cut(s) 516
Mph1103I ATGCAT 1 cut(s) 623
MroXI GAANNNNTTC 1 cut(s) 378
MscI TGGCCA 1 cut(s) 516
MseI TTAA 1 cut(s) 390
Msp20I TGGCCA 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 165
MspI CCGG 3 cut(s) 34, 73, 102
MspR9I CCNGG 3 cut(s) 34, 293, 417
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 2 cut(s) 293, 417
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 34
NdeII GATC 3 cut(s) 124, 572, 669
NlaIII CATG 3 cut(s) 317, 621, 625
NlaIV GGNNCC 2 cut(s) 31, 462
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 1 cut(s) 621
PaeI GCATGC 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PctI GAATGC 1 cut(s) 603
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 4 cut(s) 161, 164, 546, 616
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 259
Psp6I CCWGG 2 cut(s) 291, 415
PspGI CCWGG 2 cut(s) 291, 415
PspN4I GGNNCC 2 cut(s) 31, 462
PspOMI GGGCCC 1 cut(s) 460
PspPI GGNCC 2 cut(s) 460, 461
PstI CTGCAG 1 cut(s) 193
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
SacI GAGCTC 1 cut(s) 259
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 4 cut(s) 160, 163, 545, 615
Sau3AI GATC 3 cut(s) 124, 572, 669
Sau96I GGNCC 2 cut(s) 460, 461
ScaI AGTACT 1 cut(s) 187
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 3 cut(s) 34, 293, 417
SduI GDGCHC 4 cut(s) 34, 259, 271, 464
SfcI CTRYAG 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
SphI GCATGC 1 cut(s) 621
Sse9I AATT 3 cut(s) 358, 653, 691
SsiI CCGC 1 cut(s) 165
SspMI CTAG 1 cut(s) 90
SstI GAGCTC 1 cut(s) 259
StyD4I CCNGG 3 cut(s) 32, 291, 415
TaaI ACNGT 3 cut(s) 43, 66, 283
TaiI ACGT 1 cut(s) 89
TaqI TCGA 3 cut(s) 77, 261, 533
TasI AATT 3 cut(s) 358, 653, 691
TatI WGTACW 1 cut(s) 185
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 3 cut(s) 69, 286, 639
TseI GCWGC 4 cut(s) 159, 162, 544, 614
TspDTI ATGAA 2 cut(s) 302, 492
TspRI CASTG 3 cut(s) 69, 286, 639
XapI RAATTY 1 cut(s) 691
XbaI TCTAGA 1 cut(s) 89
XceI RCATGY 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 1 cut(s) 90
ZrmI AGTACT 1 cut(s) 187
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.