FvH4_4g30380

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003271
Physical Location & Seq
Reverse (-)
30045438 .. 30046887
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30380.t1

Sequence Viewer

Length: 1200 bp
ATGGCTAAAGCCCTCATCAGTAGCGACAATTTTTCCAGGAACAAGAAAAAGCAGCAGGAAACTGCTGTTGTTATCTTGAATAGTAACCAGGATGTTGTGTTTTCGATTCTGGACAAGGTAGAACCCATTGACCGTCTCCGCTTTACTGCAGTTTGTAAGGAATGGCTGGCTCTTTCGAAAAAATATTACCTCACGTCTCAACGATGGAGCACTCAGTTGGTTCCTATGCTCTTGATCCTTAAGGAGGGAAAGCTAAGACTCTACAATGTCTGTGCCGGAAAAATTGACAACAACATTCAGTTATCAGTTCTTACTGTTAAGAGGTTCTGCGGTTCTAGCCGTGGTTGGTTAGCCACAGTAGCCAAAGATCCCACAGATCCCACAGTAGATGCATGTGAATATTATCGACTGTACAAATATGATGTGATTATTCAACTCTGGAACCCTTTCGATAAAGCAACATGTCCTCTTTGTCTTCCTCCCTTGGAACTCGGTTGCGGAACATCAATTCGGAAGGTTATCCTGTTACTTGATCCATTAAGAGATAACAATTATATAGTCGCAGCAATCACAGATTCCAAGTTGGCTCTCTTCAGAGCAGGCCAAAAAGATTGGACTCGCATCCAGGCATTAGGAAAGTGTAATGATCTTATACTCTACAAAGGTCGTATCTATGGAGCTCAAAACAATGGAAGTCTTTGGTCACTGGATGTTAATCACAGCAGTACTGAAACTCGTCCAAAACTAGTTACTACCTTGAATCCCAGAGTTTATAAGCGAGTGCATATTGTAAATTCAAATACCACAGGAGACCTATTACTTCTTCAGAGAAGTTGTGAGCTGCAAACGCCATATCCCGACGAGCCCTACAGCTTCAAGATTTACAAGTTGGTGATAGATGACGATGGATCTCTTGTGCGACAAGTTGAGATTAAAAGCATAGGAGATGAGGTTATATTCGTAGGTGGAGGCTATCAATCTGTTTCTGTTTTCGCTTCCAAGTTCCCCGGATGTCAACCAAATTCTATATATTACACAACTGATCTTGGAGAATCTCCAAAAACTATAGGTGTCTGCAATTTACAAGATGGAACCATCAGTAAACTAGACCCTTTTGATGGCAGCAAGCTCATGCGTCTAGCACTTTGGATTTTGCCAAGGTGTAATGGACTCTCACTCTCATATCTTCTGAATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

400

Amino Acids

44.88

Weight (kDa)

8.98

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 99 - 358 3.4e-42 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
AciI CCGC 3 cut(s) 139, 330, 498
AclWI GGATC 5 cut(s) 229, 362, 371, 527, 916
AcsI RAATTY 3 cut(s) 793, 1021, 1192
AcuI CTGAAG 2 cut(s) 577, 809
AfaI GTAC 2 cut(s) 413, 727
AfiI CCNNNNNNNGG 2 cut(s) 244, 1118
AflII CTTAAG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 5 cut(s) 79, 434, 760, 798, 877
AhlI ACTAGT 1 cut(s) 745
AjiI CACGTC 1 cut(s) 195
AjnI CCWGG 3 cut(s) 35, 87, 624
AluBI AGCT 5 cut(s) 253, 680, 841, 873, 1129
AluI AGCT 5 cut(s) 253, 680, 841, 873, 1129
Alw21I GWGCWC 2 cut(s) 212, 682
Alw26I GTCTC 3 cut(s) 140, 201, 804
AlwI GGATC 5 cut(s) 229, 362, 371, 527, 916
AoxI GGCC 1 cut(s) 601
ApeKI GCWGC 4 cut(s) 52, 563, 841, 1122
ApoI RAATTY 3 cut(s) 793, 1021, 1192
Asp700I GAANNNNTTC 1 cut(s) 446
AsuC2I CCSGG 1 cut(s) 1008
AsuHPI GGTGA 1 cut(s) 904
AsuII TTCGAA 1 cut(s) 176
BanII GRGCYC 2 cut(s) 682, 867
BbsI GAAGAC 1 cut(s) 467
Bbv12I GWGCWC 2 cut(s) 212, 682
BbvI GCAGC 4 cut(s) 64, 575, 828, 1134
BccI CCATC 5 cut(s) 198, 899, 1082, 1103, 1112
BceAI ACGGC 1 cut(s) 324
BciT130I CCWGG 3 cut(s) 37, 89, 626
BcnI CCSGG 1 cut(s) 1008
BcoDI GTCTC 3 cut(s) 140, 201, 804
BcuI ACTAGT 1 cut(s) 745
BfaI CTAG 4 cut(s) 336, 746, 1106, 1139
BfmI CTRYAG 3 cut(s) 147, 868, 1065
BfrI CTTAAG 1 cut(s) 239
BisI GCNGC 4 cut(s) 53, 564, 842, 1123
BlsI GCNGC 4 cut(s) 54, 565, 843, 1124
BmcAI AGTACT 1 cut(s) 727
Bme1390I CCNGG 4 cut(s) 37, 89, 626, 1008
BmgBI CACGTC 1 cut(s) 195
BmiI GGNNCC 3 cut(s) 222, 443, 1093
BmrFI CCNGG 4 cut(s) 37, 89, 626, 1008
BmsI GCATC 2 cut(s) 379, 630
BpiI GAAGAC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 1008
BsaBI GATNNNNATC 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 804
BsaJI CCNNGG 4 cut(s) 340, 483, 1006, 1157
BsaXI ACNNNNNCTCC 2 cut(s) 936, 966
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 1118
Bse1I ACTGG 1 cut(s) 711
Bse8I GATNNNNATC 1 cut(s) 714
BseBI CCWGG 3 cut(s) 37, 89, 626
BseDI CCNNGG 4 cut(s) 340, 483, 1006, 1157
BseGI GGATG 4 cut(s) 97, 621, 715, 1016
BseJI GATNNNNATC 1 cut(s) 714
BseLI CCNNNNNNNGG 2 cut(s) 244, 1118
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 1 cut(s) 711
BseXI GCAGC 4 cut(s) 64, 575, 828, 1134
BshFI GGCC 1 cut(s) 603
BsiHKAI GWGCWC 2 cut(s) 212, 682
BsiSI CCGG 2 cut(s) 276, 1008
BslI CCNNNNNNNGG 2 cut(s) 244, 1118
BsmAI GTCTC 3 cut(s) 140, 201, 804
BsmBI CGTCTC 2 cut(s) 140, 201
BsnI GGCC 1 cut(s) 603
Bso31I GGTCTC 1 cut(s) 804
Bsp119I TTCGAA 1 cut(s) 176
Bsp1286I GDGCHC 3 cut(s) 212, 682, 867
Bsp1407I TGTACA 1 cut(s) 411
Bsp143I GATC 7 cut(s) 234, 367, 376, 532, 646, 908, 1042
BspACI CCGC 3 cut(s) 139, 330, 498
BspANI GGCC 1 cut(s) 603
BspCNI CTCAG 1 cut(s) 226
BspLI GGNNCC 3 cut(s) 222, 443, 1093
BspMAI CTGCAG 1 cut(s) 151
BspPI GGATC 5 cut(s) 229, 362, 371, 527, 916
BspT104I TTCGAA 1 cut(s) 176
BspTI CTTAAG 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 804
BsrGI TGTACA 1 cut(s) 411
BsrI ACTGG 1 cut(s) 711
BssECI CCNNGG 4 cut(s) 340, 483, 1006, 1157
BssMI GATC 7 cut(s) 234, 367, 376, 532, 646, 908, 1042
BssT1I CCWWGG 2 cut(s) 483, 1157
Bst2UI CCWGG 3 cut(s) 37, 89, 626
Bst4CI ACNGT 5 cut(s) 134, 316, 358, 385, 411
Bst6I CTCTTC 1 cut(s) 596
BstAFI CTTAAG 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 411
BstBI TTCGAA 1 cut(s) 176
BstC8I GCNNGC 3 cut(s) 168, 601, 1127
BstDEI CTNAG 2 cut(s) 213, 254
BstDSI CCRYGG 1 cut(s) 340
BstENI CCTNNNNNAGG 1 cut(s) 242
BstF5I GGATG 4 cut(s) 97, 621, 715, 1016
BstKTI GATC 7 cut(s) 237, 370, 379, 535, 649, 911, 1045
BstMAI GTCTC 3 cut(s) 140, 201, 804
BstMBI GATC 7 cut(s) 234, 367, 376, 532, 646, 908, 1042
BstMWI GCNNNNNNNGC 3 cut(s) 336, 359, 847
BstNI CCWGG 3 cut(s) 37, 89, 626
BstNSI RCATGY 2 cut(s) 396, 465
BstSCI CCNGG 4 cut(s) 35, 87, 624, 1006
BstSFI CTRYAG 3 cut(s) 147, 868, 1065
BstV1I GCAGC 4 cut(s) 64, 575, 828, 1134
BstV2I GAAGAC 1 cut(s) 467
BstX2I RGATCY 3 cut(s) 367, 376, 908
BstYI RGATCY 3 cut(s) 367, 376, 908
BsuRI GGCC 1 cut(s) 603
BtgI CCRYGG 1 cut(s) 340
BtrI CACGTC 1 cut(s) 195
BtsCI GGATG 4 cut(s) 97, 621, 715, 1016
BtsIMutI CAGTG 1 cut(s) 704
Cac8I GCNNGC 3 cut(s) 168, 601, 1127
CseI GACGC 1 cut(s) 1124
Csp6I GTAC 2 cut(s) 412, 726
CviAII CATG 3 cut(s) 393, 462, 1132
CviQI GTAC 2 cut(s) 412, 726
DdeI CTNAG 2 cut(s) 213, 254
DpnI GATC 7 cut(s) 236, 369, 378, 534, 648, 910, 1044
DpnII GATC 7 cut(s) 234, 367, 376, 532, 646, 908, 1042
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
Ecl136II GAGCTC 1 cut(s) 680
Eco130I CCWWGG 2 cut(s) 483, 1157
Eco24I GRGCYC 2 cut(s) 682, 867
Eco31I GGTCTC 1 cut(s) 804
Eco53kI GAGCTC 1 cut(s) 680
Eco57I CTGAAG 2 cut(s) 577, 809
EcoICRI GAGCTC 1 cut(s) 680
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoRI GAATTC 1 cut(s) 1192
EcoRII CCWGG 3 cut(s) 35, 87, 624
EcoT14I CCWWGG 2 cut(s) 483, 1157
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 2 cut(s) 682, 867
ErhI CCWWGG 2 cut(s) 483, 1157
Esp3I CGTCTC 2 cut(s) 140, 201
FaeI CATG 3 cut(s) 396, 465, 1135
FatI CATG 3 cut(s) 392, 461, 1131
Fnu4HI GCNGC 4 cut(s) 53, 564, 842, 1123
FokI GGATG 4 cut(s) 104, 608, 722, 1023
FriOI GRGCYC 2 cut(s) 682, 867
Fsp4HI GCNGC 4 cut(s) 53, 564, 842, 1123
FspBI CTAG 4 cut(s) 336, 746, 1106, 1139
GluI GCNGC 4 cut(s) 53, 564, 842, 1123
HaeIII GGCC 1 cut(s) 603
HapII CCGG 2 cut(s) 276, 1008
HgaI GACGC 1 cut(s) 1124
Hin1II CATG 3 cut(s) 396, 465, 1135
HincII GTYRAC 1 cut(s) 1016
HindII GTYRAC 1 cut(s) 1016
HinfI GANTC 7 cut(s) 106, 258, 575, 616, 760, 1052, 1170
HpaII CCGG 2 cut(s) 276, 1008
HphI GGTGA 1 cut(s) 904
Hpy166II GTNNAC 2 cut(s) 1016, 1103
Hpy188I TCNGA 4 cut(s) 513, 596, 828, 1191
Hpy188III TCNNGA 6 cut(s) 76, 110, 232, 439, 857, 877
Hpy8I GTNNAC 2 cut(s) 1016, 1103
Hpy99I CGWCG 1 cut(s) 863
HpyAV CCTTC 1 cut(s) 508
HpyCH4III ACNGT 5 cut(s) 134, 316, 358, 385, 411
HpyCH4IV ACGT 1 cut(s) 194
HpyCH4V TGCA 5 cut(s) 149, 392, 784, 844, 1077
HpyF10VI GCNNNNNNNGC 3 cut(s) 336, 359, 847
HpyF3I CTNAG 2 cut(s) 213, 254
HpySE526I ACGT 1 cut(s) 194
Hsp92II CATG 3 cut(s) 396, 465, 1135
Kzo9I GATC 7 cut(s) 234, 367, 376, 532, 646, 908, 1042
LmnI GCTCC 2 cut(s) 207, 677
Lsp1109I GCAGC 4 cut(s) 64, 575, 828, 1134
LweI GCATC 2 cut(s) 379, 630
MaeI CTAG 4 cut(s) 336, 746, 1106, 1139
MaeII ACGT 1 cut(s) 194
MaeIII GTNAC 4 cut(s) 83, 525, 702, 748
MalI GATC 7 cut(s) 236, 369, 378, 534, 648, 910, 1044
MboI GATC 7 cut(s) 234, 367, 376, 532, 646, 908, 1042
MboII GAAGA 4 cut(s) 467, 583, 815, 1178
MflI RGATCY 3 cut(s) 367, 376, 908
MhlI GDGCHC 3 cut(s) 212, 682, 867
MluCI AATT 8 cut(s) 28, 282, 507, 550, 793, 1021, 1078, 1192
MlyI GAGTC 3 cut(s) 252, 610, 1164
MnlI CCTC 8 cut(s) 23, 200, 238, 315, 477, 489, 943, 962
Mph1103I ATGCAT 1 cut(s) 394
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 5 cut(s) 240, 318, 539, 714, 933
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 2 cut(s) 276, 1008
MspR9I CCNGG 4 cut(s) 37, 89, 626, 1008
MvaI CCWGG 3 cut(s) 37, 89, 626
MwoI GCNNNNNNNGC 3 cut(s) 336, 359, 847
NciI CCSGG 1 cut(s) 1008
NdeII GATC 7 cut(s) 234, 367, 376, 532, 646, 908, 1042
NlaIII CATG 3 cut(s) 396, 465, 1135
NlaIV GGNNCC 3 cut(s) 222, 443, 1093
NmuCI GTSAC 1 cut(s) 702
NsiI ATGCAT 1 cut(s) 394
NspI RCATGY 2 cut(s) 396, 465
NspV TTCGAA 1 cut(s) 176
PciI ACATGT 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 446
PfeI GAWTC 4 cut(s) 106, 575, 760, 1052
PfoI TCCNGGA 1 cut(s) 35
PkrI GCNGC 4 cut(s) 54, 565, 843, 1124
PleI GAGTC 3 cut(s) 252, 610, 1164
PpsI GAGTC 3 cut(s) 252, 610, 1164
PscI ACATGT 1 cut(s) 461
PsiI TTATAA 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 682
Psp6I CCWGG 3 cut(s) 35, 87, 624
PspGI CCWGG 3 cut(s) 35, 87, 624
PspN4I GGNNCC 3 cut(s) 222, 443, 1093
PstI CTGCAG 1 cut(s) 151
PsuI RGATCY 3 cut(s) 367, 376, 908
RsaI GTAC 2 cut(s) 413, 727
RsaNI GTAC 2 cut(s) 412, 726
SacI GAGCTC 1 cut(s) 682
SaqAI TTAA 5 cut(s) 240, 318, 539, 714, 933
SatI GCNGC 4 cut(s) 53, 564, 842, 1123
Sau3AI GATC 7 cut(s) 234, 367, 376, 532, 646, 908, 1042
ScaI AGTACT 1 cut(s) 727
SchI GAGTC 3 cut(s) 252, 610, 1164
ScrFI CCNGG 4 cut(s) 37, 89, 626, 1008
SduI GDGCHC 3 cut(s) 212, 682, 867
SfaNI GCATC 2 cut(s) 379, 630
SfcI CTRYAG 3 cut(s) 147, 868, 1065
SfuI TTCGAA 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
SpeI ACTAGT 1 cut(s) 745
Sse9I AATT 8 cut(s) 28, 282, 507, 550, 793, 1021, 1078, 1192
SsiI CCGC 3 cut(s) 139, 330, 498
SspI AATATT 2 cut(s) 185, 401
SspMI CTAG 4 cut(s) 336, 746, 1106, 1139
SstI GAGCTC 1 cut(s) 682
StyD4I CCNGG 4 cut(s) 35, 87, 624, 1006
StyI CCWWGG 2 cut(s) 483, 1157
TaaI ACNGT 5 cut(s) 134, 316, 358, 385, 411
TaiI ACGT 1 cut(s) 197
TaqI TCGA 4 cut(s) 104, 176, 406, 450
TasI AATT 8 cut(s) 28, 282, 507, 550, 793, 1021, 1078, 1192
TatI WGTACW 2 cut(s) 411, 725
TfiI GAWTC 4 cut(s) 106, 575, 760, 1052
Tru1I TTAA 5 cut(s) 240, 318, 539, 714, 933
Tru9I TTAA 5 cut(s) 240, 318, 539, 714, 933
TscAI CASTG 1 cut(s) 711
TseFI GTSAC 1 cut(s) 702
TseI GCWGC 4 cut(s) 52, 563, 841, 1122
Tsp45I GTSAC 1 cut(s) 702
TspDTI ATGAA 1 cut(s) 1185
TspRI CASTG 1 cut(s) 711
Vha464I CTTAAG 1 cut(s) 239
XagI CCTNNNNNAGG 1 cut(s) 242
XapI RAATTY 3 cut(s) 793, 1021, 1192
XceI RCATGY 2 cut(s) 396, 465
XmnI GAANNNNTTC 1 cut(s) 446
XspI CTAG 4 cut(s) 336, 746, 1106, 1139
ZrmI AGTACT 1 cut(s) 727
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.