Rmu_sc0004275.1_g000032
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004275.1
OG0003271
Physical Location & Seq
Forward (+)
126053 .. 128613
2561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004275.1_g000032.1.cds

Sequence Viewer

Length: 657 bp
atggaatcaattcctcctgaagtacctgatagtagcgttacatccaacattgtctgcgagacgtttactcagaacccagaaggtaaagagaggaccaaaaaagagtacgaggccttagcactgaactctggattttctcgattggaagtgatttgctctgcttacaatagctgggtcatggagttccacaactatggcaatggtctaaacaacttggggcatcaagagccctctcttgtgccccagaattccgatttctgtgtgtttagatgcaggctgagttcattaccagtcttgctcaaggtgttcgatgaatcgccgcggcggaggaggacttgggagtggaaccgccgtcatcgttgccggagaagtcatcgacttcgaattcggcgctgtgatctacggagaagtggtggctggaagaggcgaaagagcctggtcaacatccccaatctccgattcacttccttcaggttcagcattcttccgatctggattaggaccagccaatcgtcgaggttgattcctctggtgacgacattatggtgggcggcccgagtctggaggagagaggtagactgcctgggtcgagcgccaaagctggagccggaggtcactaggaagggagggagggagggaaagagatatcgggactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

218

Amino Acids

25.79

Weight (kDa)

10.67

Isoelectric Point (pI)

69.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 576
AccII CGCG 1 cut(s) 322
AciI CCGC 5 cut(s) 320, 322, 325, 349, 551
AcsI RAATTY 2 cut(s) 247, 384
AcuI CTGAAG 2 cut(s) 39, 454
AfaI GTAC 2 cut(s) 24, 107
AfiI CCNNNNNNNGG 1 cut(s) 561
AjnI CCWGG 2 cut(s) 435, 582
AjuI GAANNNNNNNTTGG 2 cut(s) 443, 475
AluBI AGCT 2 cut(s) 171, 601
AluI AGCT 2 cut(s) 171, 601
Alw26I GTCTC 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 555
AoxI GGCC 2 cut(s) 111, 552
ApoI RAATTY 2 cut(s) 247, 384
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 2 cut(s) 393, 595
AspS9I GGNCC 3 cut(s) 93, 501, 553
AsuHPI GGTGA 1 cut(s) 544
AsuII TTCGAA 1 cut(s) 382
AvaI CYCGRG 1 cut(s) 555
AvaII GGWCC 2 cut(s) 93, 501
BaeGI GKGCMC 1 cut(s) 243
BanII GRGCYC 1 cut(s) 231
BceAI ACGGC 1 cut(s) 336
BciT130I CCWGG 2 cut(s) 437, 584
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 2 cut(s) 618, 655
BfoI RGCGCY 2 cut(s) 394, 596
BisI GCNGC 3 cut(s) 320, 323, 552
BlsI GCNGC 3 cut(s) 321, 324, 553
Bme1390I CCNGG 2 cut(s) 437, 584
Bme18I GGWCC 2 cut(s) 93, 501
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 3 cut(s) 93, 501, 553
BmiI GGNNCC 2 cut(s) 347, 606
BmrFI CCNGG 2 cut(s) 437, 584
BmsI GCATC 2 cut(s) 229, 260
BpmI CTGGAG 2 cut(s) 583, 623
Bpu10I CCTNAGC 1 cut(s) 115
Bpu14I TTCGAA 1 cut(s) 382
BpuEI CTTGAG 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 320, 583
BsaXI ACNNNNNCTCC 2 cut(s) 397, 427
Bsc4I CCNNNNNNNGG 1 cut(s) 561
Bse1I ACTGG 1 cut(s) 290
Bse3DI GCAATG 1 cut(s) 205
BseBI CCWGG 2 cut(s) 437, 584
BseDI CCNNGG 2 cut(s) 320, 583
BseGI GGATG 2 cut(s) 41, 444
BseLI CCNNNNNNNGG 1 cut(s) 561
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 83, 269
BseNI ACTGG 1 cut(s) 290
BseRI GAGGAG 2 cut(s) 343, 580
BseSI GKGCMC 1 cut(s) 243
BseYI CCCAGC 1 cut(s) 171
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 2 cut(s) 113, 554
BsiHKCI CYCGRG 1 cut(s) 555
BsiSI CCGG 2 cut(s) 364, 608
BslI CCNNNNNNNGG 1 cut(s) 561
BsmAI GTCTC 1 cut(s) 53
BsmBI CGTCTC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 480
BsnI GGCC 2 cut(s) 113, 554
BsoBI CYCGRG 1 cut(s) 555
Bsp119I TTCGAA 1 cut(s) 382
Bsp1286I GDGCHC 2 cut(s) 231, 243
Bsp143I GATC 2 cut(s) 397, 489
BspACI CCGC 5 cut(s) 320, 322, 325, 349, 551
BspANI GGCC 2 cut(s) 113, 554
BspCNI CTCAG 2 cut(s) 82, 270
BspFNI CGCG 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 347, 606
BspT104I TTCGAA 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 205
BsrI ACTGG 1 cut(s) 290
BssECI CCNNGG 2 cut(s) 320, 583
BssMI GATC 2 cut(s) 397, 489
Bst2UI CCWGG 2 cut(s) 437, 584
Bst6I CTCTTC 1 cut(s) 416
BstBI TTCGAA 1 cut(s) 382
BstC8I GCNNGC 1 cut(s) 275
BstDEI CTNAG 3 cut(s) 69, 115, 278
BstDSI CCRYGG 1 cut(s) 320
BstF5I GGATG 2 cut(s) 41, 444
BstFNI CGCG 1 cut(s) 322
BstH2I RGCGCY 2 cut(s) 394, 596
BstHHI GCGC 2 cut(s) 393, 595
BstKTI GATC 2 cut(s) 400, 492
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 2 cut(s) 397, 489
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 2 cut(s) 437, 584
BstSCI CCNGG 2 cut(s) 435, 582
BstSLI GKGCMC 1 cut(s) 243
BstUI CGCG 1 cut(s) 322
BstXI CCANNNNNNTGG 1 cut(s) 194
BsuRI GGCC 2 cut(s) 113, 554
BtgI CCRYGG 1 cut(s) 320
BtsCI GGATG 2 cut(s) 41, 444
BtsIMutI CAGTG 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 275
CfoI GCGC 2 cut(s) 393, 595
Cfr13I GGNCC 3 cut(s) 93, 501, 553
Cfr42I CCGCGG 1 cut(s) 323
Csp6I GTAC 2 cut(s) 23, 106
CviAII CATG 1 cut(s) 178
CviQI GTAC 2 cut(s) 23, 106
DdeI CTNAG 3 cut(s) 69, 115, 278
DpnI GATC 2 cut(s) 399, 491
DpnII GATC 2 cut(s) 397, 489
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
EciI GGCGGA 1 cut(s) 340
Eco147I AGGCCT 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 231
Eco32I GATATC 1 cut(s) 647
Eco47I GGWCC 2 cut(s) 93, 501
Eco57I CTGAAG 2 cut(s) 39, 454
Eco88I CYCGRG 1 cut(s) 555
EcoRI GAATTC 2 cut(s) 247, 384
EcoRII CCWGG 2 cut(s) 435, 582
EcoRV GATATC 1 cut(s) 647
EcoT38I GRGCYC 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 1 cut(s) 181
FaiI YATR 3 cut(s) 179, 195, 544
FatI CATG 1 cut(s) 177
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 3 cut(s) 320, 323, 552
FokI GGATG 2 cut(s) 28, 431
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 3 cut(s) 320, 323, 552
FspBI CTAG 2 cut(s) 618, 655
GlaI GCGC 2 cut(s) 392, 594
GluI GCNGC 3 cut(s) 320, 323, 552
GsaI CCCAGC 1 cut(s) 175
GsuI CTGGAG 2 cut(s) 583, 623
HaeII RGCGCY 2 cut(s) 394, 596
HaeIII GGCC 2 cut(s) 113, 554
HapII CCGG 2 cut(s) 364, 608
HhaI GCGC 2 cut(s) 393, 595
Hin1II CATG 1 cut(s) 181
Hin6I GCGC 2 cut(s) 391, 593
HinP1I GCGC 2 cut(s) 391, 593
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 5 cut(s) 5, 314, 459, 523, 558
HpaII CCGG 2 cut(s) 364, 608
HphI GGTGA 1 cut(s) 544
Hpy166II GTNNAC 3 cut(s) 66, 442, 577
Hpy188I TCNGA 4 cut(s) 72, 253, 458, 489
Hpy188III TCNNGA 7 cut(s) 17, 129, 138, 224, 493, 562, 650
Hpy8I GTNNAC 3 cut(s) 66, 442, 577
Hpy99I CGWCG 1 cut(s) 517
HpyAV CCTTC 3 cut(s) 74, 478, 616
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 3 cut(s) 69, 115, 278
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 1 cut(s) 181
HspAI GCGC 2 cut(s) 391, 593
KspI CCGCGG 1 cut(s) 323
Kzo9I GATC 2 cut(s) 397, 489
LmnI GCTCC 1 cut(s) 604
LweI GCATC 2 cut(s) 229, 260
MaeI CTAG 2 cut(s) 618, 655
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 3 cut(s) 37, 532, 613
MalI GATC 2 cut(s) 399, 491
MboI GATC 2 cut(s) 397, 489
MboII GAAGA 2 cut(s) 433, 476
MhlI GDGCHC 2 cut(s) 231, 243
MluCI AATT 3 cut(s) 9, 247, 384
MlyI GAGTC 1 cut(s) 567
MmeI TCCRAC 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 9
MslI CAYNNNNRTG 2 cut(s) 192, 544
MspA1I CMGCKG 1 cut(s) 322
MspI CCGG 2 cut(s) 364, 608
MspR9I CCNGG 2 cut(s) 437, 584
Mva1269I GAATGC 1 cut(s) 480
MvaI CCWGG 2 cut(s) 437, 584
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 1 cut(s) 226
NdeII GATC 2 cut(s) 397, 489
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 2 cut(s) 347, 606
NmuCI GTSAC 2 cut(s) 532, 613
NspV TTCGAA 1 cut(s) 382
PceI AGGCCT 1 cut(s) 113
PctI GAATGC 1 cut(s) 480
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 4 cut(s) 5, 314, 459, 523
PkrI GCNGC 3 cut(s) 321, 324, 553
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
Psp6I CCWGG 2 cut(s) 435, 582
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 2 cut(s) 435, 582
PspN4I GGNNCC 2 cut(s) 347, 606
PspPI GGNCC 3 cut(s) 93, 501, 553
RsaI GTAC 2 cut(s) 24, 107
RsaNI GTAC 2 cut(s) 23, 106
RseI CAYNNNNRTG 2 cut(s) 192, 544
SacII CCGCGG 1 cut(s) 323
SatI GCNGC 3 cut(s) 320, 323, 552
Sau3AI GATC 2 cut(s) 397, 489
Sau96I GGNCC 3 cut(s) 93, 501, 553
SchI GAGTC 1 cut(s) 567
ScrFI CCNGG 2 cut(s) 437, 584
SduI GDGCHC 2 cut(s) 231, 243
SfaNI GCATC 2 cut(s) 229, 260
Sfr303I CCGCGG 1 cut(s) 323
SfuI TTCGAA 1 cut(s) 382
SgrBI CCGCGG 1 cut(s) 323
SinI GGWCC 2 cut(s) 93, 501
SmiMI CAYNNNNRTG 2 cut(s) 192, 544
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 3 cut(s) 9, 247, 384
SseBI AGGCCT 1 cut(s) 113
SsiI CCGC 5 cut(s) 320, 322, 325, 349, 551
SspMI CTAG 2 cut(s) 618, 655
StuI AGGCCT 1 cut(s) 113
StyD4I CCNGG 2 cut(s) 435, 582
TaiI ACGT 1 cut(s) 65
TaqI TCGA 6 cut(s) 139, 309, 376, 382, 515, 589
TasI AATT 3 cut(s) 9, 247, 384
TauI GCSGC 3 cut(s) 322, 325, 554
TfiI GAWTC 4 cut(s) 5, 314, 459, 523
TscAI CASTG 1 cut(s) 126
TseFI GTSAC 2 cut(s) 532, 613
Tsp45I GTSAC 2 cut(s) 532, 613
TspDTI ATGAA 2 cut(s) 273, 327
TspGWI ACGGA 1 cut(s) 418
TspRI CASTG 1 cut(s) 126
VpaK11BI GGWCC 2 cut(s) 93, 501
XapI RAATTY 2 cut(s) 247, 384
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 2 cut(s) 618, 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.