Rh5DG235000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003271
Physical Location & Seq
Forward (+)
26830692 .. 26830946
255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG235000.1

Sequence Viewer

Length: 255 bp
ATGAATCGCCGCAGCGAAGGAGGACTTGGGAGTGGAACCGCCGTCATCGTTGGCGGAGAAGTCGTCGACTTCGAATTCGGCGCTGTGATCTGCGGAGAAGTGGTGGCTGGAGGAGGCGAAGGAGCCTGGTCAACATCCTCAATCTCCGATTCACTTCCTTCAGGTTCAGCATTCTTCCGATCTGGATTAGGACCAGCCAGTCGTCGAGGTCGACTCCGATCTAGACCGAGTCGTCGAGGTCGATTCCTCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

84

Amino Acids

8.69

Weight (kDa)

11.35

Isoelectric Point (pI)

69.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 198, 231
AccI GTMKAC 2 cut(s) 66, 211
AciI CCGC 4 cut(s) 10, 39, 54, 93
AcsI RAATTY 1 cut(s) 74
AcuI CTGAAG 1 cut(s) 144
AjnI CCWGG 1 cut(s) 125
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
ApeKI GCWGC 1 cut(s) 12
ApoI RAATTY 1 cut(s) 74
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 191
AsuII TTCGAA 1 cut(s) 72
AvaII GGWCC 1 cut(s) 191
BbvI GCAGC 1 cut(s) 24
BceAI ACGGC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 127
BfaI CTAG 1 cut(s) 222
BfoI RGCGCY 1 cut(s) 84
BisI GCNGC 2 cut(s) 10, 13
BlsI GCNGC 2 cut(s) 11, 14
Bme1390I CCNGG 1 cut(s) 127
Bme18I GGWCC 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 2 cut(s) 37, 124
BmrFI CCNGG 1 cut(s) 127
BplI GAGNNNNNCTC 2 cut(s) 198, 230
BpmI CTGGAG 1 cut(s) 129
Bpu14I TTCGAA 1 cut(s) 72
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 87, 117
Bse1I ACTGG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 127
BseGI GGATG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 198
BseRI GAGGAG 1 cut(s) 126
BseXI GCAGC 1 cut(s) 24
BsmI GAATGC 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 72
Bsp143I GATC 3 cut(s) 87, 179, 218
BspACI CCGC 4 cut(s) 10, 39, 54, 93
BspLI GGNNCC 2 cut(s) 37, 124
BspT104I TTCGAA 1 cut(s) 72
BsrI ACTGG 1 cut(s) 198
BssMI GATC 3 cut(s) 87, 179, 218
Bst2UI CCWGG 1 cut(s) 127
BstBI TTCGAA 1 cut(s) 72
BstF5I GGATG 1 cut(s) 134
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 3 cut(s) 90, 182, 221
BstMBI GATC 3 cut(s) 87, 179, 218
BstNI CCWGG 1 cut(s) 127
BstSCI CCNGG 1 cut(s) 125
BstV1I GCAGC 1 cut(s) 24
BtsCI GGATG 1 cut(s) 134
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 191
CviJI RGCY 3 cut(s) 107, 125, 197
CviKI_1 RGCY 3 cut(s) 107, 125, 197
DpnI GATC 3 cut(s) 89, 181, 220
DpnII GATC 3 cut(s) 87, 179, 218
DrdI GACNNNNNNGTC 2 cut(s) 198, 231
DseDI GACNNNNNNGTC 2 cut(s) 198, 231
EciI GGCGGA 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 144
EcoRI GAATTC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 125
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FblI GTMKAC 2 cut(s) 66, 211
Fnu4HI GCNGC 2 cut(s) 10, 13
FokI GGATG 1 cut(s) 121
Fsp4HI GCNGC 2 cut(s) 10, 13
FspBI CTAG 1 cut(s) 222
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 10, 13
GsuI CTGGAG 1 cut(s) 129
HaeII RGCGCY 1 cut(s) 84
HhaI GCGC 1 cut(s) 83
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 3 cut(s) 67, 132, 212
HindII GTYRAC 3 cut(s) 67, 132, 212
HinfI GANTC 5 cut(s) 4, 149, 213, 229, 243
Hpy166II GTNNAC 3 cut(s) 67, 132, 212
Hpy188I TCNGA 3 cut(s) 148, 179, 218
Hpy188III TCNNGA 2 cut(s) 183, 222
Hpy8I GTNNAC 3 cut(s) 67, 132, 212
Hpy99I CGWCG 3 cut(s) 68, 207, 237
HpyAV CCTTC 3 cut(s) 11, 113, 168
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 3 cut(s) 87, 179, 218
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 8 cut(s) 93, 112, 139, 147, 168, 207, 211, 235
Lsp1109I GCAGC 1 cut(s) 24
MaeI CTAG 1 cut(s) 222
MalI GATC 3 cut(s) 89, 181, 220
MboI GATC 3 cut(s) 87, 179, 218
MboII GAAGA 1 cut(s) 166
MluCI AATT 1 cut(s) 74
MlyI GAGTC 2 cut(s) 207, 238
MnlI CCTC 6 cut(s) 14, 104, 107, 148, 200, 230
MspR9I CCNGG 1 cut(s) 127
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 1 cut(s) 127
NdeII GATC 3 cut(s) 87, 179, 218
NlaIV GGNNCC 2 cut(s) 37, 124
NspV TTCGAA 1 cut(s) 72
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 208, 238
PctI GAATGC 1 cut(s) 170
PfeI GAWTC 3 cut(s) 4, 149, 243
PflFI GACNNNGTC 1 cut(s) 228
PkrI GCNGC 2 cut(s) 11, 14
PleI GAGTC 2 cut(s) 207, 237
PpsI GAGTC 2 cut(s) 207, 237
Psp6I CCWGG 1 cut(s) 125
PspGI CCWGG 1 cut(s) 125
PspN4I GGNNCC 2 cut(s) 37, 124
PspPI GGNCC 1 cut(s) 191
PsyI GACNNNGTC 1 cut(s) 228
SalI GTCGAC 2 cut(s) 65, 210
SatI GCNGC 2 cut(s) 10, 13
Sau3AI GATC 3 cut(s) 87, 179, 218
Sau96I GGNCC 1 cut(s) 191
SchI GAGTC 2 cut(s) 207, 238
ScrFI CCNGG 1 cut(s) 127
SetI ASST 3 cut(s) 166, 211, 241
SfuI TTCGAA 1 cut(s) 72
SinI GGWCC 1 cut(s) 191
Sse9I AATT 1 cut(s) 74
SsiI CCGC 4 cut(s) 10, 39, 54, 93
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 125
TaqI TCGA 6 cut(s) 66, 72, 205, 211, 235, 241
TaqII GACCGA 1 cut(s) 241
TasI AATT 1 cut(s) 74
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 3 cut(s) 4, 149, 243
TseI GCWGC 1 cut(s) 12
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 228
VpaK11BI GGWCC 1 cut(s) 191
XapI RAATTY 1 cut(s) 74
XbaI TCTAGA 1 cut(s) 221
XmiI GTMKAC 2 cut(s) 66, 211
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.