RLG00000023237

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003271
Physical Location & Seq
Reverse (-)
20357248 .. 20357639
392 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023237

Sequence Viewer

Length: 324 bp
ATGAATCACCGCGGCGGAGGAGGACTTGGGAGTGGAACCGCCGTCATCGTTGGTGGAGAAGTCGTCGACTTCGAATTCGGCGCTGTGATTCGTGGAGAAGTGGTGGCTGGAGGAGGCGAAGGAGCCTGCCAGTCGTCGAGGTCGACTCCGATCTGGACTGAGTCGTCGAGATCGATTCCTCTGGTGACGACGCCGTGGTGGGCGGCCCGAGTCTGGAGGAGAGAGGTAGACTGCCTGGGTCGAGCGCCGGAGCCGGAGACTGCCTGGGTCGAGCGCCGGAGCAGGAGACGGCCTGGCTCGTGCGCCGGAGCCGGAGGTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

108

Amino Acids

11.26

Weight (kDa)

8.71

Isoelectric Point (pI)

68.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 163
AccI GTMKAC 3 cut(s) 66, 143, 228
AccII CGCG 1 cut(s) 12
AciI CCGC 5 cut(s) 10, 12, 15, 39, 203
AcsI RAATTY 1 cut(s) 74
AcyI GRCGYC 1 cut(s) 191
AfiI CCNNNNNNNGG 1 cut(s) 213
AjnI CCWGG 3 cut(s) 234, 263, 292
Alw26I GTCTC 2 cut(s) 251, 280
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 2 cut(s) 204, 290
ApoI RAATTY 1 cut(s) 74
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspLEI GCGC 4 cut(s) 83, 247, 276, 305
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 196
AsuII TTCGAA 1 cut(s) 72
AvaI CYCGRG 1 cut(s) 207
BauI CACGAG 1 cut(s) 298
BceAI ACGGC 3 cut(s) 26, 178, 305
BciT130I CCWGG 3 cut(s) 236, 265, 294
BcoDI GTCTC 2 cut(s) 251, 280
BfoI RGCGCY 3 cut(s) 84, 248, 277
BisI GCNGC 2 cut(s) 13, 204
BlsI GCNGC 2 cut(s) 14, 205
Bme1390I CCNGG 3 cut(s) 236, 265, 294
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 4 cut(s) 37, 124, 252, 310
BmrFI CCNGG 3 cut(s) 236, 265, 294
BplI GAGNNNNNCTC 2 cut(s) 130, 162
BpmI CTGGAG 2 cut(s) 129, 235
Bpu14I TTCGAA 1 cut(s) 72
Bsa29I ATCGAT 1 cut(s) 173
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 4 cut(s) 10, 194, 235, 264
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 87, 117
Bsc4I CCNNNNNNNGG 1 cut(s) 213
Bse1I ACTGG 1 cut(s) 130
BseBI CCWGG 3 cut(s) 236, 265, 294
BseCI ATCGAT 1 cut(s) 173
BseDI CCNNGG 4 cut(s) 10, 194, 235, 264
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 3 cut(s) 33, 126, 232
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 206, 292
BshVI ATCGAT 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 5 cut(s) 248, 254, 277, 306, 312
BslI CCNNNNNNNGG 1 cut(s) 213
BsmAI GTCTC 2 cut(s) 251, 280
BsmBI CGTCTC 1 cut(s) 280
BsnI GGCC 2 cut(s) 206, 292
BsoBI CYCGRG 1 cut(s) 207
Bsp119I TTCGAA 1 cut(s) 72
Bsp143I GATC 2 cut(s) 150, 170
BspACI CCGC 5 cut(s) 10, 12, 15, 39, 203
BspANI GGCC 2 cut(s) 206, 292
BspCNI CTCAG 1 cut(s) 151
BspDI ATCGAT 1 cut(s) 173
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 4 cut(s) 37, 124, 252, 310
BspT104I TTCGAA 1 cut(s) 72
BsrI ACTGG 1 cut(s) 130
BssECI CCNNGG 4 cut(s) 10, 194, 235, 264
BssMI GATC 2 cut(s) 150, 170
BssNI GRCGYC 1 cut(s) 191
BssSI CACGAG 1 cut(s) 298
Bst2BI CACGAG 1 cut(s) 298
Bst2UI CCWGG 3 cut(s) 236, 265, 294
BstACI GRCGYC 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 1 cut(s) 159
BstDSI CCRYGG 2 cut(s) 10, 194
BstFNI CGCG 1 cut(s) 12
BstH2I RGCGCY 3 cut(s) 84, 248, 277
BstHHI GCGC 4 cut(s) 83, 247, 276, 305
BstKTI GATC 2 cut(s) 153, 173
BstMAI GTCTC 2 cut(s) 251, 280
BstMBI GATC 2 cut(s) 150, 170
BstNI CCWGG 3 cut(s) 236, 265, 294
BstSCI CCNGG 3 cut(s) 234, 263, 292
BstUI CGCG 1 cut(s) 12
Bsu15I ATCGAT 1 cut(s) 173
BsuRI GGCC 2 cut(s) 206, 292
BsuTUI ATCGAT 1 cut(s) 173
BtgI CCRYGG 2 cut(s) 10, 194
Cac8I GCNNGC 1 cut(s) 127
CfoI GCGC 4 cut(s) 83, 247, 276, 305
Cfr13I GGNCC 1 cut(s) 205
Cfr42I CCGCGG 1 cut(s) 13
ClaI ATCGAT 1 cut(s) 173
CseI GACGC 1 cut(s) 199
CviJI RGCY 7 cut(s) 107, 125, 206, 253, 292, 297, 311
CviKI_1 RGCY 7 cut(s) 107, 125, 206, 253, 292, 297, 311
DdeI CTNAG 1 cut(s) 159
DpnI GATC 2 cut(s) 152, 172
DpnII GATC 2 cut(s) 150, 170
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
EciI GGCGGA 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 207
EcoRI GAATTC 1 cut(s) 74
EcoRII CCWGG 3 cut(s) 234, 263, 292
Esp3I CGTCTC 1 cut(s) 280
FblI GTMKAC 3 cut(s) 66, 143, 228
Fnu4HI GCNGC 2 cut(s) 13, 204
Fsp4HI GCNGC 2 cut(s) 13, 204
GlaI GCGC 4 cut(s) 82, 246, 275, 304
GluI GCNGC 2 cut(s) 13, 204
GsuI CTGGAG 2 cut(s) 129, 235
HaeII RGCGCY 3 cut(s) 84, 248, 277
HaeIII GGCC 2 cut(s) 206, 292
HapII CCGG 5 cut(s) 248, 254, 277, 306, 312
HgaI GACGC 1 cut(s) 199
HhaI GCGC 4 cut(s) 83, 247, 276, 305
Hin1I GRCGYC 1 cut(s) 191
Hin6I GCGC 4 cut(s) 81, 245, 274, 303
HinP1I GCGC 4 cut(s) 81, 245, 274, 303
HincII GTYRAC 2 cut(s) 67, 144
HindII GTYRAC 2 cut(s) 67, 144
HinfI GANTC 6 cut(s) 4, 88, 145, 161, 175, 210
HpaII CCGG 5 cut(s) 248, 254, 277, 306, 312
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 3 cut(s) 67, 144, 229
Hpy188I TCNGA 1 cut(s) 150
Hpy188III TCNNGA 3 cut(s) 154, 168, 214
Hpy8I GTNNAC 3 cut(s) 67, 144, 229
Hpy99I CGWCG 4 cut(s) 68, 139, 169, 193
HpyAV CCTTC 1 cut(s) 113
HpyF3I CTNAG 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 191
HspAI GCGC 4 cut(s) 81, 245, 274, 303
KspI CCGCGG 1 cut(s) 13
Kzo9I GATC 2 cut(s) 150, 170
LmnI GCTCC 4 cut(s) 122, 250, 279, 308
MaeIII GTNAC 1 cut(s) 184
MalI GATC 2 cut(s) 152, 172
MboI GATC 2 cut(s) 150, 170
MluCI AATT 1 cut(s) 74
MlyI GAGTC 3 cut(s) 139, 170, 219
MnlI CCTC 9 cut(s) 11, 14, 104, 107, 132, 189, 210, 217, 308
MspA1I CMGCKG 1 cut(s) 12
MspI CCGG 5 cut(s) 248, 254, 277, 306, 312
MspR9I CCNGG 3 cut(s) 236, 265, 294
MvaI CCWGG 3 cut(s) 236, 265, 294
MvnI CGCG 1 cut(s) 12
NdeII GATC 2 cut(s) 150, 170
NlaIV GGNNCC 4 cut(s) 37, 124, 252, 310
NmuCI GTSAC 1 cut(s) 184
NspV TTCGAA 1 cut(s) 72
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 140, 170
PfeI GAWTC 3 cut(s) 4, 88, 175
PflFI GACNNNGTC 1 cut(s) 160
PkrI GCNGC 2 cut(s) 14, 205
PleI GAGTC 3 cut(s) 139, 169, 218
PpsI GAGTC 3 cut(s) 139, 169, 218
Psp6I CCWGG 3 cut(s) 234, 263, 292
PspGI CCWGG 3 cut(s) 234, 263, 292
PspN4I GGNNCC 4 cut(s) 37, 124, 252, 310
PspPI GGNCC 1 cut(s) 205
PsyI GACNNNGTC 1 cut(s) 160
SacII CCGCGG 1 cut(s) 13
SalI GTCGAC 2 cut(s) 65, 142
SatI GCNGC 2 cut(s) 13, 204
Sau3AI GATC 2 cut(s) 150, 170
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 3 cut(s) 139, 170, 219
ScrFI CCNGG 3 cut(s) 236, 265, 294
SetI ASST 3 cut(s) 143, 228, 319
Sfr303I CCGCGG 1 cut(s) 13
SfuI TTCGAA 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 13
Sse9I AATT 1 cut(s) 74
SsiI CCGC 5 cut(s) 10, 12, 15, 39, 203
StyD4I CCNGG 3 cut(s) 234, 263, 292
TaqI TCGA 8 cut(s) 66, 72, 137, 143, 167, 173, 241, 270
TasI AATT 1 cut(s) 74
TauI GCSGC 2 cut(s) 15, 206
TfiI GAWTC 3 cut(s) 4, 88, 175
TseFI GTSAC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 160
XapI RAATTY 1 cut(s) 74
XmiI GTMKAC 3 cut(s) 66, 143, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.