RLG00000009321

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003271
Physical Location & Seq
Forward (+)
50985444 .. 50985888
445 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009321

Sequence Viewer

Length: 357 bp
ATGAATCACCGCGGCGGAGGAGGACTTGGGAGTGGAACCGCCGTCATCGTTGGTGGAGAAGTCGTCGACTTCGAATTCGGCGCTGTGATCCGTGGAGAAGTGGACCAGCCAGTCGTCAAGGTCGACTCCGATCTGGACCGAGTCGTCGAGATCGATTCCTCTGGTGACGACGCCGTGGTGGGCGGCCCGAGTCTGGAGGAGAAAGGTAGACTGCCTGGGTCGAGCGCCGGAGCCAGAGACTGCCTGGGTCGAGCGCCGGAGCAGGAGACGGCCTGGCTCGTGCGCCGGAGCCGGAGGTCAATAGGAAGGGAAGGAGGGACGGAGAGAGATATCGGGACTAGAGAGAGATTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

12.44

Weight (kDa)

4.91

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 110, 143
AccI GTMKAC 3 cut(s) 66, 123, 208
AccII CGCG 1 cut(s) 12
AciI CCGC 5 cut(s) 10, 12, 15, 39, 183
AclWI GGATC 1 cut(s) 82
AcsI RAATTY 1 cut(s) 74
AcyI GRCGYC 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 193
AjnI CCWGG 3 cut(s) 214, 243, 272
Alw26I GTCTC 2 cut(s) 231, 260
AlwI GGATC 1 cut(s) 82
AlwNI CAGNNNCTG 1 cut(s) 240
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 2 cut(s) 184, 270
ApoI RAATTY 1 cut(s) 74
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspLEI GCGC 4 cut(s) 83, 227, 256, 285
AspS9I GGNCC 3 cut(s) 103, 136, 185
AsuHPI GGTGA 1 cut(s) 176
AsuII TTCGAA 1 cut(s) 72
AvaI CYCGRG 1 cut(s) 187
AvaII GGWCC 2 cut(s) 103, 136
BauI CACGAG 1 cut(s) 278
BceAI ACGGC 3 cut(s) 26, 158, 285
BciT130I CCWGG 3 cut(s) 216, 245, 274
BcoDI GTCTC 2 cut(s) 231, 260
BfaI CTAG 1 cut(s) 339
BfoI RGCGCY 3 cut(s) 84, 228, 257
BisI GCNGC 2 cut(s) 13, 184
BlsI GCNGC 2 cut(s) 14, 185
Bme1390I CCNGG 3 cut(s) 216, 245, 274
Bme18I GGWCC 2 cut(s) 103, 136
BmeT110I CYCGRG 1 cut(s) 187
BmgT120I GGNCC 3 cut(s) 103, 136, 185
BmiI GGNNCC 3 cut(s) 37, 232, 290
BmrFI CCNGG 3 cut(s) 216, 245, 274
BpmI CTGGAG 1 cut(s) 215
Bpu14I TTCGAA 1 cut(s) 72
Bsa29I ATCGAT 1 cut(s) 153
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 5 cut(s) 10, 91, 174, 215, 244
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 110
BseBI CCWGG 3 cut(s) 216, 245, 274
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 5 cut(s) 10, 91, 174, 215, 244
BseLI CCNNNNNNNGG 1 cut(s) 193
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 2 cut(s) 33, 212
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 186, 272
BshVI ATCGAT 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 187
BsiSI CCGG 4 cut(s) 228, 257, 286, 292
BslFI GGGAC 2 cut(s) 331, 349
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 2 cut(s) 231, 260
BsmBI CGTCTC 1 cut(s) 260
BsmFI GGGAC 2 cut(s) 331, 349
BsnI GGCC 2 cut(s) 186, 272
BsoBI CYCGRG 1 cut(s) 187
Bsp119I TTCGAA 1 cut(s) 72
Bsp143I GATC 3 cut(s) 87, 130, 150
BspACI CCGC 5 cut(s) 10, 12, 15, 39, 183
BspANI GGCC 2 cut(s) 186, 272
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 3 cut(s) 37, 232, 290
BspPI GGATC 1 cut(s) 82
BspT104I TTCGAA 1 cut(s) 72
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 5 cut(s) 10, 91, 174, 215, 244
BssMI GATC 3 cut(s) 87, 130, 150
BssNI GRCGYC 1 cut(s) 171
BssSI CACGAG 1 cut(s) 278
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 3 cut(s) 216, 245, 274
BstACI GRCGYC 1 cut(s) 171
BstBI TTCGAA 1 cut(s) 72
BstDSI CCRYGG 3 cut(s) 10, 91, 174
BstFNI CGCG 1 cut(s) 12
BstH2I RGCGCY 3 cut(s) 84, 228, 257
BstHHI GCGC 4 cut(s) 83, 227, 256, 285
BstKTI GATC 3 cut(s) 90, 133, 153
BstMAI GTCTC 2 cut(s) 231, 260
BstMBI GATC 3 cut(s) 87, 130, 150
BstNI CCWGG 3 cut(s) 216, 245, 274
BstSCI CCNGG 3 cut(s) 214, 243, 272
BstUI CGCG 1 cut(s) 12
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 2 cut(s) 186, 272
BsuTUI ATCGAT 1 cut(s) 153
BtgI CCRYGG 3 cut(s) 10, 91, 174
CaiI CAGNNNCTG 1 cut(s) 240
CfoI GCGC 4 cut(s) 83, 227, 256, 285
Cfr13I GGNCC 3 cut(s) 103, 136, 185
Cfr42I CCGCGG 1 cut(s) 13
ClaI ATCGAT 1 cut(s) 153
CseI GACGC 1 cut(s) 179
CviJI RGCY 6 cut(s) 109, 186, 233, 272, 277, 291
CviKI_1 RGCY 6 cut(s) 109, 186, 233, 272, 277, 291
DpnI GATC 3 cut(s) 89, 132, 152
DpnII GATC 3 cut(s) 87, 130, 150
DrdI GACNNNNNNGTC 2 cut(s) 110, 143
DseDI GACNNNNNNGTC 2 cut(s) 110, 143
EciI GGCGGA 1 cut(s) 30
Eco32I GATATC 1 cut(s) 331
Eco47I GGWCC 2 cut(s) 103, 136
Eco88I CYCGRG 1 cut(s) 187
EcoRI GAATTC 1 cut(s) 74
EcoRII CCWGG 3 cut(s) 214, 243, 272
EcoRV GATATC 1 cut(s) 331
Esp3I CGTCTC 1 cut(s) 260
FaiI YATR 1 cut(s) 352
FaqI GGGAC 2 cut(s) 331, 349
FblI GTMKAC 3 cut(s) 66, 123, 208
Fnu4HI GCNGC 2 cut(s) 13, 184
Fsp4HI GCNGC 2 cut(s) 13, 184
FspBI CTAG 1 cut(s) 339
GlaI GCGC 4 cut(s) 82, 226, 255, 284
GluI GCNGC 2 cut(s) 13, 184
GsuI CTGGAG 1 cut(s) 215
HaeII RGCGCY 3 cut(s) 84, 228, 257
HaeIII GGCC 2 cut(s) 186, 272
HapII CCGG 4 cut(s) 228, 257, 286, 292
HgaI GACGC 1 cut(s) 179
HhaI GCGC 4 cut(s) 83, 227, 256, 285
Hin1I GRCGYC 1 cut(s) 171
Hin6I GCGC 4 cut(s) 81, 225, 254, 283
HinP1I GCGC 4 cut(s) 81, 225, 254, 283
HincII GTYRAC 2 cut(s) 67, 124
HindII GTYRAC 2 cut(s) 67, 124
HinfI GANTC 5 cut(s) 4, 125, 141, 155, 190
HpaII CCGG 4 cut(s) 228, 257, 286, 292
HphI GGTGA 1 cut(s) 176
Hpy166II GTNNAC 4 cut(s) 67, 103, 124, 209
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 4 cut(s) 134, 148, 194, 334
Hpy8I GTNNAC 4 cut(s) 67, 103, 124, 209
Hpy99I CGWCG 3 cut(s) 68, 149, 173
HpyAV CCTTC 2 cut(s) 300, 305
Hsp92I GRCGYC 1 cut(s) 171
HspAI GCGC 4 cut(s) 81, 225, 254, 283
KspI CCGCGG 1 cut(s) 13
Kzo9I GATC 3 cut(s) 87, 130, 150
LmnI GCTCC 3 cut(s) 230, 259, 288
MaeI CTAG 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 164
MalI GATC 3 cut(s) 89, 132, 152
MboI GATC 3 cut(s) 87, 130, 150
MluCI AATT 1 cut(s) 74
MlyI GAGTC 3 cut(s) 119, 150, 199
MnlI CCTC 6 cut(s) 11, 14, 169, 190, 288, 308
MspA1I CMGCKG 1 cut(s) 12
MspI CCGG 4 cut(s) 228, 257, 286, 292
MspR9I CCNGG 3 cut(s) 216, 245, 274
MvaI CCWGG 3 cut(s) 216, 245, 274
MvnI CGCG 1 cut(s) 12
NdeII GATC 3 cut(s) 87, 130, 150
NlaIV GGNNCC 3 cut(s) 37, 232, 290
NmuCI GTSAC 1 cut(s) 164
NspV TTCGAA 1 cut(s) 72
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 120, 150
PfeI GAWTC 2 cut(s) 4, 155
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 14, 185
PleI GAGTC 3 cut(s) 119, 149, 198
PpsI GAGTC 3 cut(s) 119, 149, 198
Psp6I CCWGG 3 cut(s) 214, 243, 272
PspGI CCWGG 3 cut(s) 214, 243, 272
PspN4I GGNNCC 3 cut(s) 37, 232, 290
PspPI GGNCC 3 cut(s) 103, 136, 185
PstNI CAGNNNCTG 1 cut(s) 240
PsyI GACNNNGTC 1 cut(s) 140
SacII CCGCGG 1 cut(s) 13
SalI GTCGAC 2 cut(s) 65, 122
SatI GCNGC 2 cut(s) 13, 184
Sau3AI GATC 3 cut(s) 87, 130, 150
Sau96I GGNCC 3 cut(s) 103, 136, 185
SchI GAGTC 3 cut(s) 119, 150, 199
ScrFI CCNGG 3 cut(s) 216, 245, 274
SetI ASST 3 cut(s) 123, 208, 299
Sfr303I CCGCGG 1 cut(s) 13
SfuI TTCGAA 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 13
SinI GGWCC 2 cut(s) 103, 136
Sse9I AATT 1 cut(s) 74
SsiI CCGC 5 cut(s) 10, 12, 15, 39, 183
SspMI CTAG 1 cut(s) 339
StyD4I CCNGG 3 cut(s) 214, 243, 272
TaqI TCGA 7 cut(s) 66, 72, 123, 147, 153, 221, 250
TaqII GACCGA 1 cut(s) 153
TasI AATT 1 cut(s) 74
TauI GCSGC 2 cut(s) 15, 186
TfiI GAWTC 2 cut(s) 4, 155
TseFI GTSAC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 80, 335
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 103, 136
XapI RAATTY 1 cut(s) 74
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XmiI GTMKAC 3 cut(s) 66, 123, 208
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.