FvH4_4g31991

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006930
Physical Location & Seq
Reverse (-)
30859287 .. 30861682
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31991.t1

Sequence Viewer

Length: 975 bp
ATGGCAAACAACAATCAGTACGTCCTGGTAATCATGTTATTCTTAGATCTTTGCCTAATCTATGGTCTCTCCTCAGCTGTGGGTGAAGATGGAACCGGAACAAGTTCACAGAAAATGGTAAATGTTGGGGTAATTCTTGACCTGGATTCTCCGGTTGGCAAGACGGCTCAACGCTGCATATCCATGGCCGTCTTGGATTTCTATGCTAGAAATCCCAACTATCGAACAAAGCTGAATCTTACTGTGAAGGATTCTAGGAATGATGTTGTTTCTGCAGCATCAGCAGTTTGGGCATTAGCCAAGGCTGTAGAACTTGTTGAGTTGCAGGCAAATTATTCCAGTGCTCTAAAGAAAAATGATATAGGGAAAAGCACAGGTGGAATATTGAGCCAAATTGGTGTGTCTAAGACAGGTCCAAGGCTTCTCCAAACATTGTTAAGTACCAAATTTGAAGGCTTAAGTGGGAGAATTAGATTGATTGATCAACAGTTTCAACCTGCAGCCTTTGAAATATTCAATGTGATACGTAGGAGGGAGAGAGTGATTGGATATTGGACCCCCAACAAAGAATCATTTCATGATTTAGTTCAATCCAGTATGTTAACAAATTACTCGAGTAATTCCATTGCCATGGATGATCATGGTGAGTTTTTCGTTAGAAAACCTGTATGGCCTGGAAACACCAGTACTCCACCAAAGGGTTGGGCTATACCTATAACTGGTAAAAAGTTGAGGATTGGTGTACCTGCAATAACAGCTAACAGTTTTGATGAATTTATGAAGGTAGAATGGTTTCCTAATACCAATGAGCCTAAGAGCTTCTCAGGGTTTTCATATGATGTCTTCGTTGCAGCCTTAGAAAGAATGCCATTAGCACTTCCACACAAGTTCATTCCATTCACCAATGGTTCATCAAGAGAGGGTGCAGGGACTTATGACGATCTTCTTCACCAGATAAAATTGAATGCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

325

Amino Acids

35.75

Weight (kDa)

9.07

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 95 - 175 6.6e-06 Receptor family ligand binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 505, 754
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 2 cut(s) 446, 773
AfaI GTAC 4 cut(s) 20, 442, 688, 744
AfiI CCNNNNNNNGG 2 cut(s) 698, 719
AflII CTTAAG 1 cut(s) 457
AgsI TTSAA 6 cut(s) 452, 494, 509, 517, 590, 964
AjnI CCWGG 3 cut(s) 24, 141, 673
AluBI AGCT 4 cut(s) 77, 232, 758, 819
AluI AGCT 4 cut(s) 77, 232, 758, 819
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 613
AoxI GGCC 2 cut(s) 186, 671
ApeKI GCWGC 4 cut(s) 174, 275, 500, 851
ApoI RAATTY 2 cut(s) 446, 773
Asp700I GAANNNNTTC 3 cut(s) 103, 573, 792
AspS9I GGNCC 2 cut(s) 413, 555
AsuHPI GGTGA 4 cut(s) 95, 656, 892, 941
AvaI CYCGRG 1 cut(s) 613
AvaII GGWCC 2 cut(s) 413, 555
BbsI GAAGAC 1 cut(s) 835
Bbv12I GWGCWC 1 cut(s) 346
BbvCI CCTCAGC 1 cut(s) 73
BbvI GCAGC 4 cut(s) 161, 287, 512, 863
BccI CCATC 1 cut(s) 83
BceAI ACGGC 2 cut(s) 173, 180
BciT130I CCWGG 3 cut(s) 26, 143, 675
BclI TGATCA 2 cut(s) 481, 637
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 2 cut(s) 207, 255
BfmI CTRYAG 3 cut(s) 273, 306, 498
BfrI CTTAAG 1 cut(s) 457
BfuAI ACCTGC 2 cut(s) 505, 754
BglII AGATCT 1 cut(s) 46
BisI GCNGC 4 cut(s) 175, 276, 501, 852
BlsI GCNGC 4 cut(s) 176, 277, 502, 853
BmcAI AGTACT 1 cut(s) 688
Bme1390I CCNGG 3 cut(s) 26, 143, 675
Bme18I GGWCC 2 cut(s) 413, 555
BmeT110I CYCGRG 1 cut(s) 613
BmgT120I GGNCC 2 cut(s) 413, 555
BmiI GGNNCC 2 cut(s) 94, 557
BmrFI CCNGG 3 cut(s) 26, 143, 675
BmsI GCATC 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 835
Bpu10I CCTNAGC 1 cut(s) 73
BsaAI YACGTR 1 cut(s) 527
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 4 cut(s) 183, 300, 416, 630
BsaWI WCCGGW 2 cut(s) 95, 151
BsaXI ACNNNNNCTCC 2 cut(s) 673, 703
Bsc4I CCNNNNNNNGG 2 cut(s) 698, 719
Bse1I ACTGG 4 cut(s) 339, 594, 684, 724
Bse3DI GCAATG 1 cut(s) 624
BseBI CCWGG 3 cut(s) 26, 143, 675
BseDI CCNNGG 4 cut(s) 183, 300, 416, 630
BseGI GGATG 1 cut(s) 640
BseLI CCNNNNNNNGG 2 cut(s) 698, 719
BseMI GCAATG 1 cut(s) 624
BseMII CTCAG 2 cut(s) 87, 837
BseNI ACTGG 4 cut(s) 339, 594, 684, 724
BseRI GAGGAG 1 cut(s) 61
BseXI GCAGC 4 cut(s) 161, 287, 512, 863
BsgI GTGCAG 1 cut(s) 945
BshFI GGCC 2 cut(s) 188, 673
BsiHKAI GWGCWC 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 613
BsiSI CCGG 2 cut(s) 96, 152
BslFI GGGAC 1 cut(s) 943
BslI CCNNNNNNNGG 2 cut(s) 698, 719
BsmAI GTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 943
BsmI GAATGC 2 cut(s) 870, 970
BsnI GGCC 2 cut(s) 188, 673
Bso31I GGTCTC 1 cut(s) 71
BsoBI CYCGRG 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 4 cut(s) 46, 481, 637, 940
Bsp19I CCATGG 2 cut(s) 183, 630
BspANI GGCC 2 cut(s) 188, 673
BspCNI CTCAG 2 cut(s) 86, 836
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 2 cut(s) 94, 557
BspMAI CTGCAG 2 cut(s) 277, 502
BspMI ACCTGC 2 cut(s) 505, 754
BspTI CTTAAG 1 cut(s) 457
BspTNI GGTCTC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 624
BsrI ACTGG 4 cut(s) 339, 594, 684, 724
BssECI CCNNGG 4 cut(s) 183, 300, 416, 630
BssMI GATC 4 cut(s) 46, 481, 637, 940
BssT1I CCWWGG 4 cut(s) 183, 300, 416, 630
Bst2UI CCWGG 3 cut(s) 26, 143, 675
Bst4CI ACNGT 3 cut(s) 244, 489, 764
BstAFI CTTAAG 1 cut(s) 457
BstBAI YACGTR 1 cut(s) 527
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 6 cut(s) 43, 73, 405, 813, 823, 856
BstDSI CCRYGG 2 cut(s) 183, 630
BstF5I GGATG 1 cut(s) 640
BstKTI GATC 4 cut(s) 49, 484, 640, 943
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 4 cut(s) 46, 481, 637, 940
BstMWI GCNNNNNNNGC 3 cut(s) 281, 290, 755
BstNI CCWGG 3 cut(s) 26, 143, 675
BstSCI CCNGG 3 cut(s) 24, 141, 673
BstSFI CTRYAG 3 cut(s) 273, 306, 498
BstSNI TACGTA 1 cut(s) 527
BstV1I GCAGC 4 cut(s) 161, 287, 512, 863
BstV2I GAAGAC 1 cut(s) 835
BstX2I RGATCY 1 cut(s) 46
BstXI CCANNNNNNTGG 2 cut(s) 631, 702
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 2 cut(s) 188, 673
BtgI CCRYGG 2 cut(s) 183, 630
BtsCI GGATG 1 cut(s) 640
BtsIMutI CAGTG 1 cut(s) 346
BveI ACCTGC 2 cut(s) 505, 754
Cac8I GCNNGC 1 cut(s) 327
CciI TCATGA 1 cut(s) 577
Cfr13I GGNCC 2 cut(s) 413, 555
Csp6I GTAC 4 cut(s) 19, 441, 687, 743
CviAII CATG 5 cut(s) 34, 184, 578, 631, 641
CviQI GTAC 4 cut(s) 19, 441, 687, 743
DdeI CTNAG 6 cut(s) 43, 73, 405, 813, 823, 856
DpnI GATC 4 cut(s) 48, 483, 639, 942
DpnII GATC 4 cut(s) 46, 481, 637, 940
EaeI YGGCCR 1 cut(s) 186
Eco105I TACGTA 1 cut(s) 527
Eco130I CCWWGG 4 cut(s) 183, 300, 416, 630
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 2 cut(s) 413, 555
Eco88I CYCGRG 1 cut(s) 613
EcoRII CCWGG 3 cut(s) 24, 141, 673
EcoT14I CCWWGG 4 cut(s) 183, 300, 416, 630
ErhI CCWWGG 4 cut(s) 183, 300, 416, 630
FaeI CATG 5 cut(s) 37, 187, 581, 634, 644
FaqI GGGAC 1 cut(s) 943
FatI CATG 5 cut(s) 33, 183, 577, 630, 640
FauNDI CATATG 1 cut(s) 835
FbaI TGATCA 2 cut(s) 481, 637
Fnu4HI GCNGC 4 cut(s) 175, 276, 501, 852
FokI GGATG 1 cut(s) 647
Fsp4HI GCNGC 4 cut(s) 175, 276, 501, 852
FspBI CTAG 2 cut(s) 207, 255
GluI GCNGC 4 cut(s) 175, 276, 501, 852
HaeIII GGCC 2 cut(s) 188, 673
HapII CCGG 2 cut(s) 96, 152
Hin1II CATG 5 cut(s) 37, 187, 581, 634, 644
HincII GTYRAC 1 cut(s) 603
HindII GTYRAC 1 cut(s) 603
HinfI GANTC 4 cut(s) 146, 235, 251, 569
HpaI GTTAAC 1 cut(s) 603
HpaII CCGG 2 cut(s) 96, 152
HphI GGTGA 4 cut(s) 95, 656, 892, 941
Hpy166II GTNNAC 3 cut(s) 107, 603, 743
Hpy188III TCNNGA 3 cut(s) 137, 578, 915
Hpy8I GTNNAC 3 cut(s) 107, 603, 743
HpyAV CCTTC 3 cut(s) 241, 446, 775
HpyCH4III ACNGT 3 cut(s) 244, 489, 764
HpyCH4IV ACGT 2 cut(s) 21, 526
HpyCH4V TGCA 8 cut(s) 177, 275, 325, 500, 749, 851, 926, 968
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 290, 755
HpyF3I CTNAG 6 cut(s) 43, 73, 405, 813, 823, 856
HpySE526I ACGT 2 cut(s) 21, 526
Hsp92II CATG 5 cut(s) 37, 187, 581, 634, 644
Ksp22I TGATCA 2 cut(s) 481, 637
KspAI GTTAAC 1 cut(s) 603
Kzo9I GATC 4 cut(s) 46, 481, 637, 940
Lsp1109I GCAGC 4 cut(s) 161, 287, 512, 863
LweI GCATC 1 cut(s) 287
MaeI CTAG 2 cut(s) 207, 255
MaeII ACGT 2 cut(s) 21, 526
MalI GATC 4 cut(s) 48, 483, 639, 942
MboI GATC 4 cut(s) 46, 481, 637, 940
MboII GAAGA 4 cut(s) 98, 835, 935, 938
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 346
MluCI AATT 9 cut(s) 132, 331, 393, 446, 468, 607, 619, 773, 959
MnlI CCTC 4 cut(s) 82, 525, 726, 913
MroXI GAANNNNTTC 3 cut(s) 103, 573, 792
MseI TTAA 4 cut(s) 437, 458, 602, 973
MslI CAYNNNNRTG 2 cut(s) 182, 629
MspA1I CMGCKG 1 cut(s) 77
MspCI CTTAAG 1 cut(s) 457
MspI CCGG 2 cut(s) 96, 152
MspR9I CCNGG 3 cut(s) 26, 143, 675
Mva1269I GAATGC 2 cut(s) 870, 970
MvaI CCWGG 3 cut(s) 26, 143, 675
MwoI GCNNNNNNNGC 3 cut(s) 281, 290, 755
NcoI CCATGG 2 cut(s) 183, 630
NdeI CATATG 1 cut(s) 835
NdeII GATC 4 cut(s) 46, 481, 637, 940
NlaIII CATG 5 cut(s) 37, 187, 581, 634, 644
NlaIV GGNNCC 2 cut(s) 94, 557
PaeR7I CTCGAG 1 cut(s) 613
PagI TCATGA 1 cut(s) 577
PctI GAATGC 2 cut(s) 870, 970
PdmI GAANNNNTTC 3 cut(s) 103, 573, 792
PfeI GAWTC 4 cut(s) 146, 235, 251, 569
PkrI GCNGC 4 cut(s) 176, 277, 502, 853
Ppu21I YACGTR 1 cut(s) 527
Psp6I CCWGG 3 cut(s) 24, 141, 673
PspGI CCWGG 3 cut(s) 24, 141, 673
PspN4I GGNNCC 2 cut(s) 94, 557
PspPI GGNCC 2 cut(s) 413, 555
PspXI VCTCGAGB 1 cut(s) 613
PstI CTGCAG 2 cut(s) 277, 502
PsuI RGATCY 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 4 cut(s) 20, 442, 688, 744
RsaNI GTAC 4 cut(s) 19, 441, 687, 743
RseI CAYNNNNRTG 2 cut(s) 182, 629
SaqAI TTAA 4 cut(s) 437, 458, 602, 973
SatI GCNGC 4 cut(s) 175, 276, 501, 852
Sau3AI GATC 4 cut(s) 46, 481, 637, 940
Sau96I GGNCC 2 cut(s) 413, 555
ScaI AGTACT 1 cut(s) 688
ScrFI CCNGG 3 cut(s) 26, 143, 675
SduI GDGCHC 1 cut(s) 346
SfaNI GCATC 1 cut(s) 287
SfcI CTRYAG 3 cut(s) 273, 306, 498
Sfr274I CTCGAG 1 cut(s) 613
SinI GGWCC 2 cut(s) 413, 555
SlaI CTCGAG 1 cut(s) 613
SmiMI CAYNNNNRTG 2 cut(s) 182, 629
SmlI CTYRAG 2 cut(s) 457, 613
SmoI CTYRAG 2 cut(s) 457, 613
SnaBI TACGTA 1 cut(s) 527
Sse9I AATT 9 cut(s) 132, 331, 393, 446, 468, 607, 619, 773, 959
SspI AATATT 2 cut(s) 384, 513
SspMI CTAG 2 cut(s) 207, 255
StyD4I CCNGG 3 cut(s) 24, 141, 673
StyI CCWWGG 4 cut(s) 183, 300, 416, 630
TaaI ACNGT 3 cut(s) 244, 489, 764
TaiI ACGT 2 cut(s) 24, 529
TaqI TCGA 2 cut(s) 223, 614
TasI AATT 9 cut(s) 132, 331, 393, 446, 468, 607, 619, 773, 959
TatI WGTACW 1 cut(s) 686
TfiI GAWTC 4 cut(s) 146, 235, 251, 569
Tru1I TTAA 4 cut(s) 437, 458, 602, 973
Tru9I TTAA 4 cut(s) 437, 458, 602, 973
TscAI CASTG 1 cut(s) 346
TseI GCWGC 4 cut(s) 174, 275, 500, 851
TspDTI ATGAA 6 cut(s) 566, 786, 794, 822, 880, 900
TspRI CASTG 1 cut(s) 346
Vha464I CTTAAG 1 cut(s) 457
VpaK11BI GGWCC 2 cut(s) 413, 555
XapI RAATTY 2 cut(s) 446, 773
XcmI CCANNNNNNNNNTGG 2 cut(s) 190, 699
XhoI CTCGAG 1 cut(s) 613
XmnI GAANNNNTTC 3 cut(s) 103, 573, 792
XspI CTAG 2 cut(s) 207, 255
ZrmI AGTACT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.