RchiOBHm_Chr4g0404091

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006930
Physical Location & Seq
Forward (+)
23621919 .. 23625992
4074 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37571

Sequence Viewer

Length: 3198 bp
ATGGCGAACAACAATCAGTACCTTCTGATAATCTTCATGTTATTAGATGTTTGCCTAATCTGTGGCAACTCCTCAGCTGTTGGTGAAGATGGAACCGGAAGAAAATCACAGCAAATGGTAAATGTGGGCGTAATTCTTGACCTGGGTACTCCGGTTGGAAAGACAGCTCAACGCTGCATATCAATGGCACTCTCGGAATTCTATGCTAGAAATGCCAACTATCGAACAAAGCTGAATCTTACCGTGAAGGATTCTAGGAATGATGTTGTTTCTGCAGCATCAGCAGCTTTAGAATTGCTAAATAATGAAGAGGTGCAAGCTATTATAGGTCCCCAAACATCAAACCAAGCTAAGTTTGTCACAAGTCTTGGAGAGAAAGCACAGATCCCCATTATCTCGTTTTCAGCCACAAGCCCTTCTTTGTCTCCAACTATGAACCGTTTCTTCATCAGGACAGCTCTGGATGACTCTTTTCAAGTAAAAGCCATCACATCCATTGTTAAAGCATATGGGTGGCATGAAGTTGTTCTTATATATGAAGACACAGAATATGGAAATGGTTTGATTCCCTATATAATTGATGCCTTTCAACAAATTGACACCAGGGTTCCTTACAGAAGTGTCATTTCCCCATCATCCAGTCGCTTAAACATTTTGAGAAAGCTTAACAAGTTAATGGCAATGGAGACGAGGGTATTCTTGGTGCACATGACTCCTTCACTTGGTTCTAAACTCTTTGTACTTGCAAAAGATGCAGGCATGATGAGTGAAGGGTATGCTTGGATCATAACTGATGGATTATCATCTGTGTTACATCCACTGGAGGGAAATGTCATTGATTCAATGGAAGGTGTGGTGGGCGTAACGCCCTATGTACCAATGTCAACAGGTCTTGATGACTTTGAGATGAGATGGAAACAAAGCACTATTGGGACTACAAAGCAGACTCATTCTAAGATAAGCATATTTGGCTTATGGGCTTATGACACAGTTTGGGCATTAGCTAAGGCTGTAGAACTCGTTGAGTTGCAGGCAAATTATTCCAGTGCTTTAAAGAACAATAATAAAGGGAAAAGCTCAGGTGGAATATTGAGCCAAATTGGTGTGTCTAAATCAGGTCCAAGGCTTCTCCAAACATTGCTGAGGACCAGATTTGAAGGCTTAAGTGGAAGATTTAGATTGATTGATCAACAATTTCAACCTGCAGCCTTCAAAATTTTCAATGTGATAGGTAAGAGGGAGAGAGTGATTGGATATTGGACCCCCAACAAAGAATCATTTGATGATTTAGTTCAATCTGGTATGTTAACAAATTACTCGAGTACTTCGATTGCCATAGATGATCATCGTGAGTTTATCATTAAAGTACCTATATGGCCTGGAAACACCAGTACTCCACCAAAGGGTTGGGATATACCTGTAACTGGTAAAAAGTTGAGGATTGGTGTACCTGTAACATCTGACAGTTATAATGAATTTATGAAGATAAAATGGTTCCCTCACACCAATGAGCCTAAGAGCTTCTCAGGGTTCTCGTATGATGTTTTCCTTGCAGCCTTAGAAAGAGTGCCATTTGCACTTCCACACAAGTTCATTCCATTCACCAATGTTTCATCAAGAGAGGCTGCAGGGACTTATGACGATCTTCTTTACCAGATTAAGTTGAATAAATTTGATGCTGTAGTGGGAGATATAACAATAATTGCCAGTAGGACGTCTTATGTTGATTTTACACTACCTTATTCTGAATCAGGCGTACGGATGGTGGTGAAAGTTAAAGATGACCAAAGAAGGAATATGTGGATTTTCCTAAAACCTCTTAGTTGGGATCTTTGGTTGACAACTGGGGGTGCCTTCATTTTTACAGCTGTTGTAGTATGGGTCTTGGAACACAATCAAAACACAGAGTTCAAGGGTTCACGACAGCAACAAGTTGGCACAACTCTATGGTTTTCTTTTTCAACTCTTGTTTTTGCACACAAGGAGAGGGTGGTGAGCAATTGGACAAGATTTGTGCTGATCATATGGATTTTTGTGGTACTCATCCTAACACAGAGTTACACTGCAAGCTTAGCGTCACTGCTAACTGTAGAAAGGTTGCAGCCTGCAGTTGTTGATATAAATGAACTCAGACGTAATGGTGATTATGTTGGATACCAAACGAATTCCTTTGTACGAGAGCTTTTAACAAGAGAATTGAAGTTTGAGGAGAACAGGCTAAAGAATTATAGAGTTATGGAGGATTATCATAAAGCATTGTCTCAAGGAAGCAAAAATGGTGGTGTTGATGCTATTTTCGACGAAATCCCCTACGTGAAGCTCTTCCTCGCCAAGTATTCTGCTGGATACACTATGGTTGGACCAACCTACAAAACTGACGGATTTGGCTTTGCCTTCCCACTAGGCTCCCCTCTTGTTGCTTACTTTTCGAGGGCAATCTTGAAAGTGACTCAAGATCATGAGACAATGGAACAACTGCAACGGAAGTATTTCCCAGATGAATCCAAATGTCAAGATAAGAGTTCCACATTCTCTTCAGAAAATCCAAGTCTAAGCGTGTACAGCTTTGCAGGCCTGTTCCTCACCACAGCGGTCATTTCAATTTCTTCATGCTCGATATCTATGCTTTCCAAGTTCCTGTATAAACAGTGGCCTGCGTTGAATCCCATCCATGCAGAAGCCAGCTCACTCTTGTCAAAAATATCAAAGCATTTTCATCAAAACAAAGCAACTGATCTAAAGATAACTGCTTCATCTGCAACAAATGCTAGAGGTGGTGAAGCTTCACAGATGCTAGAGTGTTTCCACCAAGAACTAGACAATGTTCCAACAGATGAACATAAGGTGCTGTCCAGAGCAGTTAGCAGGCGTTTAGATGAAAGAGATTCGGAAATGAAGAGGATTGAAGAGGAGCAGAGAAGGATTAGGGAGGGCCATGAAGTGAGTTTGGCTCTTCTTCACAGGGCTGAACTTGATAACTTACTTAAGGTGCCTGTGCAGGAGATCATAGCAGTGGAGATGGACAAGGCCATACAGAGGGCTGGGTTGTTACACTTGGGACCTTCTTTGGCTGCCCTGCTCGGAATGGCAGGTGAAACGAACAGAAGTATATCACAGTCGACAAACCAGGGGAATGAAGTGAATGCATCAACCAGTACTATTGCTAATCCGGATTATGTGGCAAAGCCTGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

1065

Amino Acids

119.09

Weight (kDa)

8.47

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 40 - 339 2.5e-23 Periplasmic binding protein
ANF_receptor PF01094 57 - 411 4.4e-80 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 501 - 593 1.5e-07 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 544 - 832 1.8e-08 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 608 - 865 2.1e-37 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1470
AarI CACCTGC 1 cut(s) 3080
AatII GACGTC 1 cut(s) 1718
Acc36I ACCTGC 2 cut(s) 1210, 3080
AccB1I GGYRCC 2 cut(s) 1850, 2989
AccI GTMKAC 1 cut(s) 3119
AccIII TCCGGA 1 cut(s) 3169
AciI CCGC 1 cut(s) 2591
AclWI GGATC 3 cut(s) 379, 791, 1836
AcsI RAATTY 5 cut(s) 197, 1215, 1475, 1670, 2164
AcuI CTGAAG 1 cut(s) 2520
AcyI GRCGYC 1 cut(s) 1715
AfiI CCNNNNNNNGG 5 cut(s) 722, 824, 1403, 1424, 3036
AflII CTTAAG 2 cut(s) 1162, 2984
AjnI CCWGG 4 cut(s) 141, 602, 1378, 3126
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 4 cut(s) 429, 680, 2265, 2456
Alw44I GTGCAC 1 cut(s) 704
AlwI GGATC 3 cut(s) 379, 791, 1836
Ama87I CYCGRG 1 cut(s) 1318
Aor13HI TCCGGA 1 cut(s) 3169
AoxI GGCC 5 cut(s) 1376, 2572, 2651, 2932, 3027
ApaLI GTGCAC 1 cut(s) 704
ApeKI GCWGC 8 cut(s) 174, 275, 284, 1205, 1553, 1625, 2101, 3071
ApoI RAATTY 5 cut(s) 197, 1215, 1475, 1670, 2164
Asp700I GAANNNNTTC 4 cut(s) 440, 525, 2321, 2489
AspS9I GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2360, 2932, 3059
AsuHPI GGTGA 8 cut(s) 95, 1594, 1780, 2005, 2153, 2575, 2789, 3104
AvaI CYCGRG 1 cut(s) 1318
AvaII GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2360, 3059
BaeGI GKGCMC 1 cut(s) 708
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BanI GGYRCC 2 cut(s) 1850, 2989
BbsI GAAGAC 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 708
BbvCI CCTCAGC 2 cut(s) 73, 1142
BbvI GCAGC 8 cut(s) 161, 287, 296, 1217, 1565, 1612, 2113, 3058
BccI CCATC 8 cut(s) 83, 494, 640, 788, 906, 1756, 2675, 3013
BcgI CGANNNNNNTGC 2 cut(s) 2605, 2639
BciT130I CCWGG 4 cut(s) 143, 604, 1380, 3128
BciVI GTATCC 2 cut(s) 2147, 2339
BclI TGATCA 3 cut(s) 1186, 1342, 2019
BcoDI GTCTC 4 cut(s) 429, 680, 2265, 2456
BfaI CTAG 6 cut(s) 207, 255, 2402, 2769, 2795, 2816
BfmI CTRYAG 7 cut(s) 273, 1011, 1203, 1626, 1680, 2088, 2106
BfrI CTTAAG 2 cut(s) 1162, 2984
BfuAI ACCTGC 2 cut(s) 1210, 3080
BfuI GTATCC 2 cut(s) 2147, 2339
BisI GCNGC 8 cut(s) 175, 276, 285, 1206, 1554, 1626, 2102, 3072
BlpI GCTNAGC 1 cut(s) 2071
BlsI GCNGC 8 cut(s) 176, 277, 286, 1207, 1555, 1627, 2103, 3073
BmcAI AGTACT 3 cut(s) 1324, 1393, 3157
Bme1390I CCNGG 4 cut(s) 143, 604, 1380, 3128
Bme18I GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2360, 3059
BmeT110I CYCGRG 1 cut(s) 1318
BmgT120I GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2360, 2932, 3059
BmiI GGNNCC 9 cut(s) 94, 331, 609, 1262, 1496, 1852, 2407, 2991, 3060
BmrFI CCNGG 4 cut(s) 143, 604, 1380, 3128
BmrI ACTGGG 1 cut(s) 1854
BmsI GCATC 7 cut(s) 287, 571, 742, 1666, 2278, 2781, 3155
BmuI ACTGGG 1 cut(s) 1854
BpiI GAAGAC 1 cut(s) 546
BplI GAGNNNNNCTC 2 cut(s) 2935, 2967
BpmI CTGGAG 1 cut(s) 842
Bpu10I CCTNAGC 4 cut(s) 73, 1005, 1078, 1142
Bpu1102I GCTNAGC 1 cut(s) 2071
BpuEI CTTGAG 2 cut(s) 2247, 2436
BsaAI YACGTR 1 cut(s) 2314
BsaBI GATNNNNATC 2 cut(s) 802, 1344
BsaHI GRCGYC 1 cut(s) 1715
BsaJI CCNNGG 4 cut(s) 142, 603, 1121, 3127
BsaWI WCCGGW 3 cut(s) 95, 151, 3169
BsaXI ACNNNNNCTCC 2 cut(s) 1378, 1408
Bsc4I CCNNNNNNNGG 5 cut(s) 722, 824, 1403, 1424, 3036
Bse1I ACTGG 8 cut(s) 639, 825, 1044, 1389, 1429, 1707, 1849, 3153
Bse3DI GCAATG 2 cut(s) 687, 1136
Bse8I GATNNNNATC 2 cut(s) 802, 1344
BseAI TCCGGA 1 cut(s) 3169
BseBI CCWGG 4 cut(s) 143, 604, 1380, 3128
BseDI CCNNGG 4 cut(s) 142, 603, 1121, 3127
BseGI GGATG 7 cut(s) 469, 491, 635, 814, 1767, 2043, 2667
BseJI GATNNNNATC 2 cut(s) 802, 1344
BseLI CCNNNNNNNGG 5 cut(s) 722, 824, 1403, 1424, 3036
BseMI GCAATG 2 cut(s) 687, 1136
BseMII CTCAG 5 cut(s) 87, 1092, 1133, 1539, 2143
BseNI ACTGG 8 cut(s) 639, 825, 1044, 1389, 1429, 1707, 1849, 3153
BseRI GAGGAG 3 cut(s) 61, 2222, 2924
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 8 cut(s) 161, 287, 296, 1217, 1565, 1612, 2113, 3058
BseYI CCCAGC 1 cut(s) 3041
BsgI GTGCAG 1 cut(s) 3017
BshFI GGCC 5 cut(s) 1378, 2574, 2653, 2934, 3029
BshNI GGYRCC 2 cut(s) 1850, 2989
BsiHKAI GWGCWC 1 cut(s) 708
BsiHKCI CYCGRG 1 cut(s) 1318
BsiSI CCGG 3 cut(s) 96, 152, 3170
BsiWI CGTACG 1 cut(s) 1756
BslFI GGGAC 4 cut(s) 315, 946, 1645, 3072
BslI CCNNNNNNNGG 5 cut(s) 722, 824, 1403, 1424, 3036
BsmAI GTCTC 4 cut(s) 429, 680, 2265, 2456
BsmBI CGTCTC 1 cut(s) 680
BsmFI GGGAC 4 cut(s) 315, 946, 1645, 3072
BsmI GAATGC 1 cut(s) 3148
BsnI GGCC 5 cut(s) 1378, 2574, 2653, 2934, 3029
BsoBI CYCGRG 1 cut(s) 1318
Bsp1286I GDGCHC 1 cut(s) 708
Bsp13I TCCGGA 1 cut(s) 3169
Bsp1407I TGTACA 1 cut(s) 2559
Bsp1720I GCTNAGC 1 cut(s) 2071
BspACI CCGC 1 cut(s) 2591
BspANI GGCC 5 cut(s) 1378, 2574, 2653, 2934, 3029
BspCNI CTCAG 5 cut(s) 86, 1091, 1134, 1538, 2142
BspEI TCCGGA 1 cut(s) 3169
BspHI TCATGA 1 cut(s) 2458
BspLI GGNNCC 9 cut(s) 94, 331, 609, 1262, 1496, 1852, 2407, 2991, 3060
BspMAI CTGCAG 4 cut(s) 277, 1207, 1630, 2110
BspMI ACCTGC 2 cut(s) 1210, 3080
BspPI GGATC 3 cut(s) 379, 791, 1836
BspQI GCTCTTC 2 cut(s) 2327, 2958
BspT107I GGYRCC 2 cut(s) 1850, 2989
BspTI CTTAAG 2 cut(s) 1162, 2984
BsrDI GCAATG 2 cut(s) 687, 1136
BsrGI TGTACA 1 cut(s) 2559
BsrI ACTGG 8 cut(s) 639, 825, 1044, 1389, 1429, 1707, 1849, 3153
BssECI CCNNGG 4 cut(s) 142, 603, 1121, 3127
BssNI GRCGYC 1 cut(s) 1715
BssT1I CCWWGG 1 cut(s) 1121
Bst2UI CCWGG 4 cut(s) 143, 604, 1380, 3128
Bst4CI ACNGT 7 cut(s) 244, 440, 991, 1466, 2089, 2649, 3117
Bst6I CTCTTC 6 cut(s) 303, 2327, 2539, 2891, 2901, 2958
BstACI GRCGYC 1 cut(s) 1715
BstAFI CTTAAG 2 cut(s) 1162, 2984
BstAPI GCANNNNNTGC 2 cut(s) 752, 2765
BstAUI TGTACA 1 cut(s) 2559
BstBAI YACGTR 1 cut(s) 2314
BstC8I GCNNGC 9 cut(s) 318, 757, 1032, 2068, 2106, 2572, 2655, 2683, 2867
BstF5I GGATG 7 cut(s) 469, 491, 635, 814, 1767, 2043, 2667
BstMAI GTCTC 4 cut(s) 429, 680, 2265, 2456
BstNI CCWGG 4 cut(s) 143, 604, 1380, 3128
BstSCI CCNGG 4 cut(s) 141, 602, 1378, 3126
BstSFI CTRYAG 7 cut(s) 273, 1011, 1203, 1626, 1680, 2088, 2106
BstSLI GKGCMC 1 cut(s) 708
BstV1I GCAGC 8 cut(s) 161, 287, 296, 1217, 1565, 1612, 2113, 3058
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 2 cut(s) 384, 1828
BstXI CCANNNNNNTGG 1 cut(s) 1407
BstYI RGATCY 2 cut(s) 384, 1828
BsuI GTATCC 2 cut(s) 2147, 2339
BsuRI GGCC 5 cut(s) 1378, 2574, 2653, 2934, 3029
BtsCI GGATG 7 cut(s) 469, 491, 635, 814, 1767, 2043, 2667
BtsI GCAGTG 3 cut(s) 2061, 2078, 3018
BtsIMutI CAGTG 6 cut(s) 818, 1051, 2061, 2078, 2654, 3018
BveI ACCTGC 2 cut(s) 1210, 3080
Cac8I GCNNGC 9 cut(s) 318, 757, 1032, 2068, 2106, 2572, 2655, 2683, 2867
CciI TCATGA 1 cut(s) 2458
Cfr13I GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2360, 2932, 3059
CseI GACGC 1 cut(s) 2064
CspCI CAANNNNNGTGG 2 cut(s) 2260, 2295
CviAII CATG 8 cut(s) 37, 518, 709, 760, 2459, 2610, 2672, 2936
DraI TTTAAA 1 cut(s) 1053
Eam1104I CTCTTC 6 cut(s) 303, 2327, 2539, 2891, 2901, 2958
EarI CTCTTC 6 cut(s) 303, 2327, 2539, 2891, 2901, 2958
Eco130I CCWWGG 1 cut(s) 1121
Eco147I AGGCCT 1 cut(s) 2574
Eco32I GATATC 1 cut(s) 2619
Eco47I GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2360, 3059
Eco57I CTGAAG 1 cut(s) 2520
Eco88I CYCGRG 1 cut(s) 1318
EcoO109I RGGNCCY 2 cut(s) 329, 3059
EcoRI GAATTC 2 cut(s) 197, 2164
EcoRII CCWGG 4 cut(s) 141, 602, 1378, 3126
EcoRV GATATC 1 cut(s) 2619
EcoT14I CCWWGG 1 cut(s) 1121
EcoT22I ATGCAT 1 cut(s) 3148
ErhI CCWWGG 1 cut(s) 1121
Esp3I CGTCTC 1 cut(s) 680
FaeI CATG 8 cut(s) 40, 521, 712, 763, 2462, 2613, 2675, 2939
FalI AAGNNNNNCTT 4 cut(s) 403, 435, 513, 545
FaqI GGGAC 4 cut(s) 315, 946, 1645, 3072
FatI CATG 8 cut(s) 36, 517, 708, 759, 2458, 2609, 2671, 2935
FauNDI CATATG 2 cut(s) 508, 2024
FbaI TGATCA 3 cut(s) 1186, 1342, 2019
FblI GTMKAC 1 cut(s) 3119
Fnu4HI GCNGC 8 cut(s) 175, 276, 285, 1206, 1554, 1626, 2102, 3072
FokI GGATG 7 cut(s) 476, 478, 622, 801, 1774, 2030, 2654
Fsp4HI GCNGC 8 cut(s) 175, 276, 285, 1206, 1554, 1626, 2102, 3072
FspBI CTAG 6 cut(s) 207, 255, 2402, 2769, 2795, 2816
GluI GCNGC 8 cut(s) 175, 276, 285, 1206, 1554, 1626, 2102, 3072
GsaI CCCAGC 1 cut(s) 3045
GsuI CTGGAG 1 cut(s) 842
HaeIII GGCC 5 cut(s) 1378, 2574, 2653, 2934, 3029
HapII CCGG 3 cut(s) 96, 152, 3170
HgaI GACGC 1 cut(s) 2064
Hin1I GRCGYC 1 cut(s) 1715
Hin1II CATG 8 cut(s) 40, 521, 712, 763, 2462, 2613, 2675, 2939
HincII GTYRAC 4 cut(s) 885, 1308, 1839, 3120
HindII GTYRAC 4 cut(s) 885, 1308, 1839, 3120
HindIII AAGCTT 3 cut(s) 662, 2068, 2781
HpaI GTTAAC 1 cut(s) 1308
HpaII CCGG 3 cut(s) 96, 152, 3170
HphI GGTGA 8 cut(s) 95, 1594, 1780, 2005, 2153, 2575, 2789, 3104
Hpy166II GTNNAC 8 cut(s) 706, 885, 1308, 1448, 1839, 1919, 2560, 3120
Hpy188I TCNGA 8 cut(s) 27, 196, 1462, 1747, 2132, 2539, 2890, 3083
Hpy8I GTNNAC 8 cut(s) 706, 885, 1308, 1448, 1839, 1919, 2560, 3120
Hpy99I CGWCG 1 cut(s) 2303
HpyCH4III ACNGT 7 cut(s) 244, 440, 991, 1466, 2089, 2649, 3117
HpyCH4IV ACGT 3 cut(s) 1715, 2134, 2313
HpySE526I ACGT 3 cut(s) 1715, 2134, 2313
Hsp92I GRCGYC 1 cut(s) 1715
Hsp92II CATG 8 cut(s) 40, 521, 712, 763, 2462, 2613, 2675, 2939
Kpn2I TCCGGA 1 cut(s) 3169
Ksp22I TGATCA 3 cut(s) 1186, 1342, 2019
KspAI GTTAAC 1 cut(s) 1308
LguI GCTCTTC 2 cut(s) 2327, 2958
LmnI GCTCC 2 cut(s) 2411, 2911
Lsp1109I GCAGC 8 cut(s) 161, 287, 296, 1217, 1565, 1612, 2113, 3058
LweI GCATC 7 cut(s) 287, 571, 742, 1666, 2278, 2781, 3155
MaeI CTAG 6 cut(s) 207, 255, 2402, 2769, 2795, 2816
MaeII ACGT 3 cut(s) 1715, 2134, 2313
MaeIII GTNAC 9 cut(s) 358, 810, 862, 1420, 1453, 2057, 2076, 2446, 3048
MfeI CAATTG 1 cut(s) 1999
MflI RGATCY 2 cut(s) 384, 1828
MhlI GDGCHC 1 cut(s) 708
MlyI GAGTC 4 cut(s) 461, 706, 940, 2443
MmeI TCCRAC 5 cut(s) 136, 452, 2131, 2338, 2852
Mph1103I ATGCAT 1 cut(s) 3148
MroI TCCGGA 1 cut(s) 3169
MroXI GAANNNNTTC 4 cut(s) 440, 525, 2321, 2489
MslI CAYNNNNRTG 4 cut(s) 182, 511, 1506, 3011
MspA1I CMGCKG 3 cut(s) 77, 1868, 2591
MspCI CTTAAG 2 cut(s) 1162, 2984
MspI CCGG 3 cut(s) 96, 152, 3170
MspR9I CCNGG 4 cut(s) 143, 604, 1380, 3128
MunI CAATTG 1 cut(s) 1999
Mva1269I GAATGC 1 cut(s) 3148
MvaI CCWGG 4 cut(s) 143, 604, 1380, 3128
NdeI CATATG 2 cut(s) 508, 2024
NlaIII CATG 8 cut(s) 40, 521, 712, 763, 2462, 2613, 2675, 2939
NlaIV GGNNCC 9 cut(s) 94, 331, 609, 1262, 1496, 1852, 2407, 2991, 3060
NmuCI GTSAC 3 cut(s) 358, 2076, 2446
NsiI ATGCAT 1 cut(s) 3148
PaeR7I CTCGAG 1 cut(s) 1318
PagI TCATGA 1 cut(s) 2458
PaqCI CACCTGC 1 cut(s) 3080
PceI AGGCCT 1 cut(s) 2574
PciSI GCTCTTC 2 cut(s) 2327, 2958
PcsI WCGNNNNNNNCGW 1 cut(s) 1325
PctI GAATGC 1 cut(s) 3148
PdmI GAANNNNTTC 4 cut(s) 440, 525, 2321, 2489
PfeI GAWTC 9 cut(s) 235, 251, 565, 839, 1274, 1748, 2501, 2662, 2885
Pfl23II CGTACG 1 cut(s) 1756
PkrI GCNGC 8 cut(s) 176, 277, 286, 1207, 1555, 1627, 2103, 3073
PleI GAGTC 4 cut(s) 461, 706, 940, 2443
PpsI GAGTC 4 cut(s) 461, 706, 940, 2443
Ppu21I YACGTR 1 cut(s) 2314
PpuMI RGGWCCY 2 cut(s) 329, 3059
PsiI TTATAA 1 cut(s) 1470
Psp5II RGGWCCY 2 cut(s) 329, 3059
Psp6I CCWGG 4 cut(s) 141, 602, 1378, 3126
PspFI CCCAGC 1 cut(s) 3041
PspGI CCWGG 4 cut(s) 141, 602, 1378, 3126
PspLI CGTACG 1 cut(s) 1756
PspN4I GGNNCC 9 cut(s) 94, 331, 609, 1262, 1496, 1852, 2407, 2991, 3060
PspPI GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2360, 2932, 3059
PspPPI RGGWCCY 2 cut(s) 329, 3059
PspXI VCTCGAGB 1 cut(s) 1318
PstI CTGCAG 4 cut(s) 277, 1207, 1630, 2110
PsuI RGATCY 2 cut(s) 384, 1828
PvuII CAGCTG 2 cut(s) 77, 1868
RseI CAYNNNNRTG 4 cut(s) 182, 511, 1506, 3011
SalI GTCGAC 1 cut(s) 3118
SapI GCTCTTC 2 cut(s) 2327, 2958
SatI GCNGC 8 cut(s) 175, 276, 285, 1206, 1554, 1626, 2102, 3072
Sau96I GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2360, 2932, 3059
ScaI AGTACT 3 cut(s) 1324, 1393, 3157
SchI GAGTC 4 cut(s) 461, 706, 940, 2443
ScrFI CCNGG 4 cut(s) 143, 604, 1380, 3128
SduI GDGCHC 1 cut(s) 708
SfaNI GCATC 7 cut(s) 287, 571, 742, 1666, 2278, 2781, 3155
SfcI CTRYAG 7 cut(s) 273, 1011, 1203, 1626, 1680, 2088, 2106
Sfr274I CTCGAG 1 cut(s) 1318
SinI GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2360, 3059
SlaI CTCGAG 1 cut(s) 1318
SmiMI CAYNNNNRTG 4 cut(s) 182, 511, 1506, 3011
SmlI CTYRAG 5 cut(s) 1162, 1318, 2262, 2451, 2984
SmoI CTYRAG 5 cut(s) 1162, 1318, 2262, 2451, 2984
SseBI AGGCCT 1 cut(s) 2574
SsiI CCGC 1 cut(s) 2591
SspI AATATT 1 cut(s) 1089
SspMI CTAG 6 cut(s) 207, 255, 2402, 2769, 2795, 2816
StuI AGGCCT 1 cut(s) 2574
StyD4I CCNGG 4 cut(s) 141, 602, 1378, 3126
StyI CCWWGG 1 cut(s) 1121
TaaI ACNGT 7 cut(s) 244, 440, 991, 1466, 2089, 2649, 3117
TaiI ACGT 3 cut(s) 1718, 2137, 2316
TaqI TCGA 7 cut(s) 223, 1319, 1328, 2298, 2429, 2615, 3119
TatI WGTACW 5 cut(s) 739, 1322, 1391, 2559, 3155
TfiI GAWTC 9 cut(s) 235, 251, 565, 839, 1274, 1748, 2501, 2662, 2885
TscAI CASTG 6 cut(s) 825, 1051, 2068, 2085, 2654, 3018
TseFI GTSAC 3 cut(s) 358, 2076, 2446
TseI GCWGC 8 cut(s) 174, 275, 284, 1205, 1553, 1625, 2101, 3071
Tsp45I GTSAC 3 cut(s) 358, 2076, 2446
TspGWI ACGGA 3 cut(s) 1774, 2394, 2497
TspRI CASTG 6 cut(s) 825, 1051, 2068, 2085, 2654, 3018
Vha464I CTTAAG 2 cut(s) 1162, 2984
VneI GTGCAC 1 cut(s) 704
VpaK11BI GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2360, 3059
XapI RAATTY 5 cut(s) 197, 1215, 1475, 1670, 2164
XcmI CCANNNNNNNNNTGG 1 cut(s) 1404
XhoI CTCGAG 1 cut(s) 1318
XmiI GTMKAC 1 cut(s) 3119
XmnI GAANNNNTTC 4 cut(s) 440, 525, 2321, 2489
XspI CTAG 6 cut(s) 207, 255, 2402, 2769, 2795, 2816
ZraI GACGTC 1 cut(s) 1716
ZrmI AGTACT 3 cut(s) 1324, 1393, 3157
Zsp2I ATGCAT 1 cut(s) 3148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.