Rh4BG115700

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006930
Physical Location & Seq
Forward (+)
19504012 .. 19506127
2116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG115700.1

Sequence Viewer

Length: 1470 bp
ATGAAGATAGAATGGTTTCCTCACACCAATGAGCCTAAGAGCTTCTCAGGGTTCTCGTATGATGTCTTCCGTGCAGCCTTAGAAAGAGTGCCATTTGCACTTCCACACAAGTTCATTCCATTCACCAATGGTTCATCAAGAGAGGCTGCAGGGACTTATGACGATCTTCTTTACCAGATTAAGTTGAATAAATTTGATGCTGTAGTGGGAGATACAACAATCACTGGCAATAGGGCATCTTATGTTGATTTTACACTACCTTATTTTGAATCAGGCGTACAGATGGTGGTGAAAGTTAAAGATGACCAAAGAAAGAATATGTGGATTTTCCTAAAACCTCTTAGTTGGGATCTTTGGTTGACAACTGGGGGTGCCTTCATCTTTATAGCTGTTGTAGTATGGGTCTTGGAACACAATCAAAACATGGAGTTCAAGGGTTCACGACAGCAACAAATTGGCACAACCCTTTGGTTTTCTTTTTCAACTCTTGTTTTTGCACACAAGGAGAGGGTGGTGAGCAATTGGACAAGATTTGTGCTGATCATATGGATTTTTCTGGTACTCATCCTAACACAGAGTTACACTGCAAGCTTAGCATCACTGCTGACTGTAGAAAGGTTGCAGCCAGCAGTTGTTGATGTAAATGAACTCAGACGTAATGGTGATTATGTTGGATACCAAAAGAATTCCTTTGTACGAGAGCTTTTGATAAGAGAATTGAAGTTTGAGGAGAACAGGCTAAAGTATTATAGATTTCCGGAGGATTATCATAAAGCATTGTCTCAAGGAAGCAAAAATGGTGGTGTTGATGCTATCTTTGACGAAATCCCTTACTTGAAGCTCTTCCTGGCCAAGTATTGTGTTGGATACACTGTTGTTGGACCAACCTACAGAACTGACGGATTTGGCTTTGCCTTCCCAATAGGCTCCCCTCTTGTTGCTTACTTTTCAAGGGCAATCTTGAACGTGACTCAAGATCATGAGACAATGGAACAACTGCAACGTAAGTATTTCCCAGATGAATCCAAATGTCAAGGTAAGAGTTCCACATTCTCTTCAGAAAATCCAAGTCTAAGCGTGCACAGCTTTGCAGGCCTGTTCATCATCACAGCAGTCATTTCAATTTCTTCATGCTCGATATATGTACTTTCCAAGTTCCTGTATAAACAGTGGCCTGTATTGAATTCCATCCATCTAGAAGCTAGCTCACTCTTGTCAAAACTATCGAAGCATTTTCATCAAAACAAAGCAACTGATCAACAGATAACTGCTTCATCTGCAACAAATGCTAGAGATGGTGAAGCTTCACAGATGCTAGAGTGTTTCCACCAAGAACTAGACAATGTTCCAACAGATGAACATAAGGTGCTGTCCAGAGCAGTTAGCAGTGGAGATGGACAAGGCCATACAGAGGGCTGGGTTGTTACACTTGGGACCTTCTTTGGCTGCCCTGCTCGGAATGGCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

489

Amino Acids

55.57

Weight (kDa)

7.68

Isoelectric Point (pI)

31.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lig_chan-Glu_bd PF10613 10 - 100 1.1e-08 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 11 - 339 3.1e-10 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 115 - 372 2.8e-38 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1455
Acc36I ACCTGC 1 cut(s) 1455
AccB1I GGYRCC 1 cut(s) 371
AccIII TCCGGA 1 cut(s) 757
AclWI GGATC 1 cut(s) 357
AcoI YGGCCR 1 cut(s) 849
AcsI RAATTY 3 cut(s) 191, 685, 1183
AcuI CTGAAG 1 cut(s) 1041
AfaI GTAC 4 cut(s) 279, 561, 696, 1146
AfiI CCNNNNNNNGG 1 cut(s) 1411
AjnI CCWGG 1 cut(s) 846
AluBI AGCT 9 cut(s) 42, 389, 591, 703, 841, 1086, 1202, 1206, 1304
AluI AGCT 9 cut(s) 42, 389, 591, 703, 841, 1086, 1202, 1206, 1304
Alw21I GWGCWC 1 cut(s) 1083
Alw26I GTCTC 2 cut(s) 786, 977
Alw44I GTGCAC 1 cut(s) 1079
AlwI GGATC 1 cut(s) 357
Aor13HI TCCGGA 1 cut(s) 757
AoxI GGCC 4 cut(s) 849, 1093, 1172, 1402
ApaLI GTGCAC 1 cut(s) 1079
ApeKI GCWGC 4 cut(s) 74, 146, 622, 1446
ApoI RAATTY 3 cut(s) 191, 685, 1183
Asp700I GAANNNNTTC 2 cut(s) 15, 842
AspS9I GGNCC 2 cut(s) 881, 1434
AsuHPI GGTGA 5 cut(s) 115, 301, 526, 674, 1310
AsuNHI GCTAGC 1 cut(s) 1202
AvaII GGWCC 2 cut(s) 881, 1434
BaeGI GKGCMC 1 cut(s) 1083
BalI TGGCCA 1 cut(s) 851
BanI GGYRCC 1 cut(s) 371
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 1083
BbvI GCAGC 4 cut(s) 86, 133, 634, 1433
BccI CCATC 5 cut(s) 277, 1196, 1200, 1289, 1388
BciT130I CCWGG 1 cut(s) 848
BciVI GTATCC 2 cut(s) 668, 860
BclI TGATCA 2 cut(s) 540, 1255
BcoDI GTCTC 2 cut(s) 786, 977
BfaI CTAG 5 cut(s) 1196, 1203, 1290, 1316, 1337
BfmI CTRYAG 4 cut(s) 147, 201, 609, 889
BfuAI ACCTGC 1 cut(s) 1455
BfuI GTATCC 2 cut(s) 668, 860
BisI GCNGC 4 cut(s) 75, 147, 623, 1447
BlpI GCTNAGC 1 cut(s) 592
BlsI GCNGC 4 cut(s) 76, 148, 624, 1448
Bme1390I CCNGG 1 cut(s) 848
Bme18I GGWCC 2 cut(s) 881, 1434
BmgT120I GGNCC 2 cut(s) 881, 1434
BmiI GGNNCC 3 cut(s) 373, 928, 1435
BmrFI CCNGG 1 cut(s) 848
BmrI ACTGGG 1 cut(s) 375
BmsI GCATC 5 cut(s) 187, 245, 605, 799, 1302
BmtI GCTAGC 1 cut(s) 1206
BmuI ACTGGG 1 cut(s) 375
BpiI GAAGAC 1 cut(s) 58
Bpu1102I GCTNAGC 1 cut(s) 592
BpuEI CTTGAG 2 cut(s) 768, 957
BsaWI WCCGGW 1 cut(s) 757
Bsc4I CCNNNNNNNGG 1 cut(s) 1411
Bse1I ACTGG 2 cut(s) 229, 370
BseAI TCCGGA 1 cut(s) 757
BseBI CCWGG 1 cut(s) 848
BseGI GGATG 2 cut(s) 564, 1188
BseLI CCNNNNNNNGG 1 cut(s) 1411
BseMII CTCAG 2 cut(s) 60, 664
BseNI ACTGG 2 cut(s) 229, 370
BseRI GAGGAG 1 cut(s) 743
BseSI GKGCMC 1 cut(s) 1083
BseXI GCAGC 4 cut(s) 86, 133, 634, 1433
BseYI CCCAGC 1 cut(s) 1416
BsgI GTGCAG 1 cut(s) 93
BshFI GGCC 4 cut(s) 851, 1095, 1174, 1404
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 1 cut(s) 1083
BsiSI CCGG 1 cut(s) 758
BslFI GGGAC 2 cut(s) 166, 1447
BslI CCNNNNNNNGG 1 cut(s) 1411
BsmAI GTCTC 2 cut(s) 786, 977
BsmFI GGGAC 2 cut(s) 166, 1447
BsnI GGCC 4 cut(s) 851, 1095, 1174, 1404
Bsp1286I GDGCHC 1 cut(s) 1083
Bsp13I TCCGGA 1 cut(s) 757
Bsp143I GATC 5 cut(s) 163, 349, 540, 976, 1255
Bsp1720I GCTNAGC 1 cut(s) 592
BspANI GGCC 4 cut(s) 851, 1095, 1174, 1404
BspCNI CTCAG 2 cut(s) 59, 663
BspEI TCCGGA 1 cut(s) 757
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 3 cut(s) 373, 928, 1435
BspMAI CTGCAG 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 1455
BspOI GCTAGC 1 cut(s) 1206
BspPI GGATC 1 cut(s) 357
BspQI GCTCTTC 1 cut(s) 848
BspT107I GGYRCC 1 cut(s) 371
BsrI ACTGG 2 cut(s) 229, 370
BssMI GATC 5 cut(s) 163, 349, 540, 976, 1255
Bst2UI CCWGG 1 cut(s) 848
Bst4CI ACNGT 3 cut(s) 610, 874, 1170
Bst6I CTCTTC 2 cut(s) 848, 1060
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstC8I GCNNGC 5 cut(s) 589, 627, 1079, 1093, 1204
BstDEI CTNAG 7 cut(s) 36, 46, 79, 341, 592, 650, 1073
BstF5I GGATG 2 cut(s) 564, 1188
BstKTI GATC 5 cut(s) 166, 352, 543, 979, 1258
BstMAI GTCTC 2 cut(s) 786, 977
BstMBI GATC 5 cut(s) 163, 349, 540, 976, 1255
BstMWI GCNNNNNNNGC 5 cut(s) 593, 1083, 1092, 1277, 1286
BstNI CCWGG 1 cut(s) 848
BstSCI CCNGG 1 cut(s) 846
BstSFI CTRYAG 4 cut(s) 147, 201, 609, 889
BstSLI GKGCMC 1 cut(s) 1083
BstV1I GCAGC 4 cut(s) 86, 133, 634, 1433
BstV2I GAAGAC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuI GTATCC 2 cut(s) 668, 860
BsuRI GGCC 4 cut(s) 851, 1095, 1174, 1404
BtsCI GGATG 2 cut(s) 564, 1188
BtsI GCAGTG 3 cut(s) 582, 599, 1393
BtsIMutI CAGTG 6 cut(s) 222, 582, 599, 870, 1175, 1393
BveI ACCTGC 1 cut(s) 1455
Cac8I GCNNGC 5 cut(s) 589, 627, 1079, 1093, 1204
CciI TCATGA 1 cut(s) 979
Cfr13I GGNCC 2 cut(s) 881, 1434
Csp6I GTAC 4 cut(s) 278, 560, 695, 1145
CspCI CAANNNNNGTGG 2 cut(s) 781, 816
CviAII CATG 3 cut(s) 424, 980, 1131
CviQI GTAC 4 cut(s) 278, 560, 695, 1145
DdeI CTNAG 7 cut(s) 36, 46, 79, 341, 592, 650, 1073
DpnI GATC 5 cut(s) 165, 351, 542, 978, 1257
DpnII GATC 5 cut(s) 163, 349, 540, 976, 1255
EaeI YGGCCR 1 cut(s) 849
Eam1104I CTCTTC 2 cut(s) 848, 1060
EarI CTCTTC 2 cut(s) 848, 1060
Eco147I AGGCCT 1 cut(s) 1095
Eco47I GGWCC 2 cut(s) 881, 1434
Eco57I CTGAAG 1 cut(s) 1041
EcoO109I RGGNCCY 1 cut(s) 1434
EcoRI GAATTC 2 cut(s) 685, 1183
EcoRII CCWGG 1 cut(s) 846
FaeI CATG 3 cut(s) 427, 983, 1134
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FaqI GGGAC 2 cut(s) 166, 1447
FatI CATG 3 cut(s) 423, 979, 1130
FauNDI CATATG 1 cut(s) 545
FbaI TGATCA 2 cut(s) 540, 1255
Fnu4HI GCNGC 4 cut(s) 75, 147, 623, 1447
FokI GGATG 2 cut(s) 551, 1175
Fsp4HI GCNGC 4 cut(s) 75, 147, 623, 1447
FspBI CTAG 5 cut(s) 1196, 1203, 1290, 1316, 1337
GluI GCNGC 4 cut(s) 75, 147, 623, 1447
GsaI CCCAGC 1 cut(s) 1420
HaeIII GGCC 4 cut(s) 851, 1095, 1174, 1404
HapII CCGG 1 cut(s) 758
Hin1II CATG 3 cut(s) 427, 983, 1134
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HindIII AAGCTT 2 cut(s) 589, 1302
HinfI GANTC 3 cut(s) 269, 970, 1022
HpaII CCGG 1 cut(s) 758
HphI GGTGA 5 cut(s) 115, 301, 526, 674, 1310
Hpy166II GTNNAC 3 cut(s) 360, 440, 1081
Hpy188I TCNGA 3 cut(s) 653, 1060, 1458
Hpy188III TCNNGA 8 cut(s) 138, 441, 758, 961, 974, 980, 1196, 1374
Hpy8I GTNNAC 3 cut(s) 360, 440, 1081
HpyAV CCTTC 3 cut(s) 385, 925, 1447
HpyCH4III ACNGT 3 cut(s) 610, 874, 1170
HpyCH4IV ACGT 3 cut(s) 655, 966, 1003
HpyF10VI GCNNNNNNNGC 5 cut(s) 593, 1083, 1092, 1277, 1286
HpyF3I CTNAG 7 cut(s) 36, 46, 79, 341, 592, 650, 1073
HpySE526I ACGT 3 cut(s) 655, 966, 1003
Hsp92II CATG 3 cut(s) 427, 983, 1134
Kpn2I TCCGGA 1 cut(s) 757
Ksp22I TGATCA 2 cut(s) 540, 1255
Kzo9I GATC 5 cut(s) 163, 349, 540, 976, 1255
LguI GCTCTTC 1 cut(s) 848
LmnI GCTCC 1 cut(s) 932
Lsp1109I GCAGC 4 cut(s) 86, 133, 634, 1433
LweI GCATC 5 cut(s) 187, 245, 605, 799, 1302
MaeI CTAG 5 cut(s) 1196, 1203, 1290, 1316, 1337
MaeII ACGT 3 cut(s) 655, 966, 1003
MaeIII GTNAC 3 cut(s) 578, 967, 1423
MalI GATC 5 cut(s) 165, 351, 542, 978, 1257
MboI GATC 5 cut(s) 163, 349, 540, 976, 1255
MboII GAAGA 6 cut(s) 16, 58, 158, 835, 1047, 1119
MfeI CAATTG 1 cut(s) 520
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 1 cut(s) 1083
MlsI TGGCCA 1 cut(s) 851
MluCI AATT 7 cut(s) 191, 453, 520, 685, 716, 1122, 1183
MluNI TGGCCA 1 cut(s) 851
MlyI GAGTC 1 cut(s) 964
MmeI TCCRAC 4 cut(s) 652, 844, 859, 1373
MnlI CCTC 8 cut(s) 30, 136, 348, 501, 721, 754, 942, 1405
Mox20I TGGCCA 1 cut(s) 851
MroI TCCGGA 1 cut(s) 757
MroXI GAANNNNTTC 2 cut(s) 15, 842
MscI TGGCCA 1 cut(s) 851
MseI TTAA 2 cut(s) 180, 297
MslI CAYNNNNRTG 1 cut(s) 27
Msp20I TGGCCA 1 cut(s) 851
MspI CCGG 1 cut(s) 758
MspR9I CCNGG 1 cut(s) 848
MunI CAATTG 1 cut(s) 520
MvaI CCWGG 1 cut(s) 848
MwoI GCNNNNNNNGC 5 cut(s) 593, 1083, 1092, 1277, 1286
NdeI CATATG 1 cut(s) 545
NdeII GATC 5 cut(s) 163, 349, 540, 976, 1255
NheI GCTAGC 1 cut(s) 1202
NlaIII CATG 3 cut(s) 427, 983, 1134
NlaIV GGNNCC 3 cut(s) 373, 928, 1435
NmuCI GTSAC 1 cut(s) 967
PagI TCATGA 1 cut(s) 979
PaqCI CACCTGC 1 cut(s) 1455
PceI AGGCCT 1 cut(s) 1095
PciSI GCTCTTC 1 cut(s) 848
PdmI GAANNNNTTC 2 cut(s) 15, 842
PfeI GAWTC 2 cut(s) 269, 1022
PkrI GCNGC 4 cut(s) 76, 148, 624, 1448
PleI GAGTC 1 cut(s) 964
PpsI GAGTC 1 cut(s) 964
PpuMI RGGWCCY 1 cut(s) 1434
Psp5II RGGWCCY 1 cut(s) 1434
Psp6I CCWGG 1 cut(s) 846
PspFI CCCAGC 1 cut(s) 1416
PspGI CCWGG 1 cut(s) 846
PspN4I GGNNCC 3 cut(s) 373, 928, 1435
PspPI GGNCC 2 cut(s) 881, 1434
PspPPI RGGWCCY 1 cut(s) 1434
PstI CTGCAG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 349
RsaI GTAC 4 cut(s) 279, 561, 696, 1146
RsaNI GTAC 4 cut(s) 278, 560, 695, 1145
RseI CAYNNNNRTG 1 cut(s) 27
SapI GCTCTTC 1 cut(s) 848
SaqAI TTAA 2 cut(s) 180, 297
SatI GCNGC 4 cut(s) 75, 147, 623, 1447
Sau3AI GATC 5 cut(s) 163, 349, 540, 976, 1255
Sau96I GGNCC 2 cut(s) 881, 1434
SchI GAGTC 1 cut(s) 964
ScrFI CCNGG 1 cut(s) 848
SduI GDGCHC 1 cut(s) 1083
SfaNI GCATC 5 cut(s) 187, 245, 605, 799, 1302
SfcI CTRYAG 4 cut(s) 147, 201, 609, 889
SinI GGWCC 2 cut(s) 881, 1434
SmiMI CAYNNNNRTG 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 783, 972
SmoI CTYRAG 2 cut(s) 783, 972
Sse9I AATT 7 cut(s) 191, 453, 520, 685, 716, 1122, 1183
SseBI AGGCCT 1 cut(s) 1095
SspMI CTAG 5 cut(s) 1196, 1203, 1290, 1316, 1337
StuI AGGCCT 1 cut(s) 1095
StyD4I CCNGG 1 cut(s) 846
TaaI ACNGT 3 cut(s) 610, 874, 1170
TaiI ACGT 3 cut(s) 658, 969, 1006
TaqI TCGA 2 cut(s) 1136, 1226
TasI AATT 7 cut(s) 191, 453, 520, 685, 716, 1122, 1183
TatI WGTACW 1 cut(s) 1144
TfiI GAWTC 2 cut(s) 269, 1022
Tru1I TTAA 2 cut(s) 180, 297
Tru9I TTAA 2 cut(s) 180, 297
TscAI CASTG 6 cut(s) 229, 589, 606, 877, 1175, 1393
TseFI GTSAC 1 cut(s) 967
TseI GCWGC 4 cut(s) 74, 146, 622, 1446
Tsp45I GTSAC 1 cut(s) 967
TspGWI ACGGA 2 cut(s) 59, 915
TspRI CASTG 6 cut(s) 229, 589, 606, 877, 1175, 1393
VneI GTGCAC 1 cut(s) 1079
VpaK11BI GGWCC 2 cut(s) 881, 1434
XapI RAATTY 3 cut(s) 191, 685, 1183
XbaI TCTAGA 1 cut(s) 1195
XmnI GAANNNNTTC 2 cut(s) 15, 842
XspI CTAG 5 cut(s) 1196, 1203, 1290, 1316, 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.