Rorug04G0046800

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006930
Physical Location & Seq
Forward (+)
7266050 .. 7266711
662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0046800.1

Sequence Viewer

Length: 426 bp
ATGGACGAGGACAACAGAAGAGGGAAGGTCCAGATGGGAGAGACCAAGGACGCCGGAGAGGTTCGATACCGGGGCGTGAGGAGGCGGCCATGGGGGAAGTTTGCGGCGGAGATTCGCGACTCCAATAGGCATGGGGCGCGTGTTTGGCTCGGGACTTTCAACACTGCGGAAGAAGCAGCCAGAGCTTATGACAGAGCTGCTTACGCCATGAGGGGTGGTTTGGCCATTTTGAATTTTCCTAACGAGTATCCTTCGGGGACTGCTGTTGGTGGTTCTTCGTCTTCGTCATCGTCGGCCGTTTCGTCGTCGTCGTCTTCGTCTTCAATGCAAGCTGGGAATCAAGTTCTGGAGCTGGAGTACTTGGATGATAGGCTGCTGGAGGAACTGCTTGATGATTGTGAAGATATGAAGAGCAAAAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

141

Amino Acids

15.52

Weight (kDa)

5.91

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 22 - 72 2.2e-16 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 117, 139
AciI CCGC 4 cut(s) 85, 104, 107, 167
AcoI YGGCCR 3 cut(s) 86, 222, 294
AcsI RAATTY 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 1 cut(s) 359
AgsI TTSAA 3 cut(s) 160, 232, 324
AluBI AGCT 4 cut(s) 185, 197, 332, 352
AluI AGCT 4 cut(s) 185, 197, 332, 352
Alw26I GTCTC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 3 cut(s) 86, 222, 294
ApeKI GCWGC 3 cut(s) 176, 197, 373
ApoI RAATTY 1 cut(s) 232
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 1 cut(s) 28
BalI TGGCCA 1 cut(s) 224
BbsI GAAGAC 3 cut(s) 273, 306, 312
BbvI GCAGC 3 cut(s) 184, 188, 360
BccI CCATC 1 cut(s) 28
BceAI ACGGC 1 cut(s) 281
BciVI GTATCC 1 cut(s) 258
BcnI CCSGG 1 cut(s) 71
BcoDI GTCTC 1 cut(s) 35
BfuI GTATCC 1 cut(s) 258
BisI GCNGC 5 cut(s) 86, 105, 177, 198, 374
BlsI GCNGC 5 cut(s) 87, 106, 178, 199, 375
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 71
BpiI GAAGAC 3 cut(s) 273, 306, 312
BpmI CTGGAG 3 cut(s) 368, 374, 398
BpuMI CCSGG 1 cut(s) 71
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 3 cut(s) 45, 70, 89
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
BseDI CCNNGG 3 cut(s) 45, 70, 89
BseGI GGATG 1 cut(s) 370
BseRI GAGGAG 1 cut(s) 94
BseX3I CGGCCG 1 cut(s) 294
BseXI GCAGC 3 cut(s) 184, 188, 360
BseYI CCCAGC 1 cut(s) 332
Bsh1236I CGCG 2 cut(s) 117, 139
Bsh1285I CGRYCG 1 cut(s) 297
BshFI GGCC 3 cut(s) 88, 224, 296
BsiEI CGRYCG 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 2 cut(s) 54, 70
BslFI GGGAC 2 cut(s) 166, 271
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 2 cut(s) 166, 271
BsnI GGCC 3 cut(s) 88, 224, 296
Bso31I GGTCTC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 149
Bsp19I CCATGG 1 cut(s) 89
Bsp68I TCGCGA 1 cut(s) 117
BspACI CCGC 4 cut(s) 85, 104, 107, 167
BspANI GGCC 3 cut(s) 88, 224, 296
BspFNI CGCG 2 cut(s) 117, 139
BspQI GCTCTTC 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 45, 70, 89
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 45, 89
Bst6I CTCTTC 2 cut(s) 13, 404
BstACI GRCGYC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 330
BstDSI CCRYGG 1 cut(s) 89
BstF5I GGATG 1 cut(s) 370
BstFNI CGCG 2 cut(s) 117, 139
BstHHI GCGC 1 cut(s) 139
BstMAI GTCTC 1 cut(s) 35
BstMCI CGRYCG 1 cut(s) 297
BstMWI GCNNNNNNNGC 5 cut(s) 136, 145, 173, 182, 203
BstSCI CCNGG 1 cut(s) 69
BstUI CGCG 2 cut(s) 117, 139
BstV1I GCAGC 3 cut(s) 184, 188, 360
BstV2I GAAGAC 3 cut(s) 273, 306, 312
BstZI CGGCCG 1 cut(s) 294
BsuI GTATCC 1 cut(s) 258
BsuRI GGCC 3 cut(s) 88, 224, 296
BtgI CCRYGG 1 cut(s) 89
BtsCI GGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 162
BtuMI TCGCGA 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 330
CfoI GCGC 1 cut(s) 139
Cfr13I GGNCC 1 cut(s) 28
CseI GACGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 358
CviAII CATG 3 cut(s) 90, 131, 208
CviQI GTAC 1 cut(s) 358
EaeI YGGCCR 3 cut(s) 86, 222, 294
EagI CGGCCG 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 13, 404
EarI CTCTTC 2 cut(s) 13, 404
EciI GGCGGA 1 cut(s) 122
EclXI CGGCCG 1 cut(s) 294
Eco130I CCWWGG 2 cut(s) 45, 89
Eco31I GGTCTC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 28
Eco52I CGGCCG 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 149
EcoT14I CCWWGG 2 cut(s) 45, 89
ErhI CCWWGG 2 cut(s) 45, 89
FaeI CATG 3 cut(s) 93, 134, 211
FaiI YATR 5 cut(s) 91, 132, 189, 209, 407
FaqI GGGAC 2 cut(s) 166, 271
FatI CATG 3 cut(s) 89, 130, 207
Fnu4HI GCNGC 5 cut(s) 86, 105, 177, 198, 374
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 5 cut(s) 86, 105, 177, 198, 374
GlaI GCGC 1 cut(s) 138
GluI GCNGC 5 cut(s) 86, 105, 177, 198, 374
GsaI CCCAGC 1 cut(s) 336
GsuI CTGGAG 3 cut(s) 368, 374, 398
HaeIII GGCC 3 cut(s) 88, 224, 296
HapII CCGG 2 cut(s) 54, 70
HgaI GACGC 1 cut(s) 59
HhaI GCGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 3 cut(s) 93, 134, 211
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HinfI GANTC 3 cut(s) 112, 119, 337
HpaII CCGG 2 cut(s) 54, 70
Hpy188III TCNNGA 4 cut(s) 31, 116, 151, 347
Hpy99I CGWCG 4 cut(s) 295, 307, 310, 313
HpyAV CCTTC 2 cut(s) 19, 261
HpyCH4V TGCA 1 cut(s) 328
HpyF10VI GCNNNNNNNGC 5 cut(s) 136, 145, 173, 182, 203
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 3 cut(s) 93, 134, 211
HspAI GCGC 1 cut(s) 137
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 8 cut(s) 44, 67, 83, 193, 318, 332, 338, 362
Lsp1109I GCAGC 3 cut(s) 184, 188, 360
MboII GAAGA 8 cut(s) 30, 182, 267, 273, 306, 312, 413, 421
MlsI TGGCCA 1 cut(s) 224
MluCI AATT 1 cut(s) 232
MluNI TGGCCA 1 cut(s) 224
MlyI GAGTC 1 cut(s) 113
MnlI CCTC 6 cut(s) 14, 52, 72, 75, 204, 373
Mox20I TGGCCA 1 cut(s) 224
MscI TGGCCA 1 cut(s) 224
Msp20I TGGCCA 1 cut(s) 224
MspI CCGG 2 cut(s) 54, 70
MspR9I CCNGG 1 cut(s) 71
MvnI CGCG 2 cut(s) 117, 139
MwoI GCNNNNNNNGC 5 cut(s) 136, 145, 173, 182, 203
NciI CCSGG 1 cut(s) 71
NcoI CCATGG 1 cut(s) 89
NlaIII CATG 3 cut(s) 93, 134, 211
NruI TCGCGA 1 cut(s) 117
PciSI GCTCTTC 1 cut(s) 404
PcsI WCGNNNNNNNCGW 3 cut(s) 299, 308, 314
PfeI GAWTC 2 cut(s) 112, 337
PkrI GCNGC 5 cut(s) 87, 106, 178, 199, 375
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 332
PspPI GGNCC 1 cut(s) 28
RruI TCGCGA 1 cut(s) 117
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 404
SatI GCNGC 5 cut(s) 86, 105, 177, 198, 374
Sau96I GGNCC 1 cut(s) 28
ScaI AGTACT 1 cut(s) 359
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 1 cut(s) 71
SetI ASST 6 cut(s) 30, 63, 187, 199, 334, 354
SinI GGWCC 1 cut(s) 28
Sse9I AATT 1 cut(s) 232
SsiI CCGC 4 cut(s) 85, 104, 107, 167
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 2 cut(s) 45, 89
TaqI TCGA 1 cut(s) 64
TasI AATT 1 cut(s) 232
TatI WGTACW 1 cut(s) 357
TauI GCSGC 2 cut(s) 88, 107
TfiI GAWTC 2 cut(s) 112, 337
TscAI CASTG 1 cut(s) 169
TseI GCWGC 3 cut(s) 176, 197, 373
TspDTI ATGAA 1 cut(s) 422
TspRI CASTG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 1 cut(s) 232
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.