Rroxscaffold_5G00348610

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006930
Physical Location & Seq
Forward (+)
20946482 .. 20950529
4048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348610.1

Sequence Viewer

Length: 2979 bp
ATGGCAAACAACAATCAGTACCTTCTGATAATCTTCATGTTATTAGATGTTTGCCTAATCTATGGCAACTCCTCAGCTGTTGGTGAAGATGGAACCGGAAGAAAATCACAGCAAATGGTAAATGTGGGCGTAATTCTTGACCTGGGTACTCCGGTTGGGAAGACGGCTCAACGCTGCATATCCATGGCGCTCTCGGAATTCTATGCTAGAAATGCCAACTATCGAACAAAGCTGAATCTTACTGTGAAGGATTCTAGGAATGATGTTGTTTCTGCAGCGTCAGCAGCTTTAGAATTGCTAAATAATGAAGAGGTGCAAGCTATTATGGGTCCCCAAACATCAAACCAAGCTAAGTTTGTCATCAGTCTTGGAGAGAAAGCACAAATCCCCATTATCTCGTTTTCAGCCACAAGCCCTTCTTTGTCTCCAACTCTGAACCGTTTCTTCATCAGGACAGCTCTAGATGACTCTTCTCAAGTAAAAGCCATCACATCCATTGTTAAAGCATATGGGTGGCATGAAGTAGTTCTTATATACGAAGACACAGAATATGGAAATGGTGCGGGCATGATGAGTGAAGGGTATGCTTGGATCATAACTGATGGATTATCATCTGGGTTACATCCACTGGAGGGAAAGGTCATTGATTCAATGGAAGGTGTGGTGGGCGTAACGCCCTATGTACCAATGTCAACAGGTCTTAATGATTTTGAGATGAGATGGAAACAAAGCACTATTGGGACTACAAAGCAGACTCATTCTAAAATAAGCATATTTGGCTTATGGGCTTATGATACAGTTTGGGCATTAGCTAAGGCTGTAGAACTCGTTGAGTTGCAGGCAAATTATTCCAGTGCTTTAAAGAACAATGATAAAGGGAAAAGCACAGGTGGAATATTGAGCCAAATTGGTGTGTCTGAATCAGGTCCAAGGCTTCTCCAAACATTGCTGAGGACCAGATTTGAAGGCTTAAGTGGGAGATTTAGATTGATTGATCAACAATTTCAACCTGCAGCCTTTCAAATATTCAATGTCATGAGTAAGAGGGAGAGAGTGATTGGATATTGGACCCCCAACAAAGAATCATTTGATGATTTAGTTCAATCTGATGCTCATCGTGAGTTTATCATTAAAGAACCTACATGGCCTGGAAACACCAGTACTCCACCAAAGGGTTGGGATATACCTGTAACTGGTAAAAAGTTGAGGATTGGTGTACCTGTAACATCTGACAGTTATAATGAATTTATGAAGATAGAATGGTTCCCTTACACCAATGAGCCTAAGAGCTTCTCAGGGTTCTCGTATGATATCTTCCTTGCAGCCTTAGAAAGAGTGCCATTTGCACTTCCACACAAGTTCATTCCATTCACCAATGGTTCATCAAGAGAGGCTGCAGGGACTTATGACGATCTTCTTTACCAGATTAAGTTGAATAAATTTGATGCTGTAGTGGGAGATACAACAATAATTGCCAGTAGGACGTCTTATGTTGATTTTACACTACCTTATTCTGAATCAGGCGTACGGATGGTGGTGAAAGTTAAAGATGACCAAAGAAGGAATATGTGGATTTTCCTAAAACCTCTTAGTTGGGATCTTTGGTTGACAACTGGGGGTGCCTTCATTTTTACAGCTGTTGTAGAATGGGTCTTGGAACACAATCAAAACACGGAGTTCAAGGGTTCACGACAGCAACAAGTTGGCACAACTCTTTGGTTTTCTTTTTCAACTCTTGTTTTTGCACACAAGGAGAGGGTGGTGAGCAATTGGACAAGATTTGTGCTGATCATATGGATTTTTGTGGTACTCATCCTAACACAGAGTTACACTGCAAGCTTAGCTTCACTGCTGACTGTAGAAAGGTTGCAGCCTGCAGTTGTTGATGTAAATGAACTCAGACGTAATGGTGATTATGTTGGATACCAAACGAATTCCTTTGTACGAGAGCTTTTAACAAGAGAATTGAAGTTTGAGGAGAACAGGCTAAAGAACTATAGAGTTATGGAGGATTATCATAAAGCATTGTCTCAAGGAAGCAAAAATGGTGGTGTTGATGCTATCTTTGATGAAATCCCCTACGTGAAGCTCTTCCTCGCCAAGTATTCTGCTCGATACACTATGGTTGGACCAACCTACAAAACTGACGGATTTGGCTTTGCCTTCCCACGAGGCTCCCCTCTTGTTGCTTACTTTTCGAGGGCAATCTTGAATGTGACTCAAGATCATGAGACAATGGAACAACTGCAACGGAAGTATTTCCCAGATGAATCCAAATGTCAAGATAAGAGTTCCACATTCTCTTCAGAAAATCCAAGTCTAAGCGTGTACAGCTTTGCAGGCCTGTTCATCATCACAGCAGTCATTTCAATTTCTTCATGTTCGATATCTATGCTTTCCAAGTTCCTGTATAAACAGTGGCCTGCATTGAATTCCATCCATGCAGAAGCCAGCTCACTCTTGTCAAAATTATCAAAGCATTTTCATCAAAACAAAGCAACTGATCAAAAGATAACTGCTTCATCTGGAACAAATGCTAGAGGTGGTGAAGCTTCACAGATGCTAGAGTCTTTCCACCAAGAACTAGATAATGTTCCAACAGATGAACATAAGGTGCTGTCCAGAGCAGTTAGCAGGCGATTAGATGAAAAAGATTCGGAAATGAAGAGGATTGAAGAGGAGAAGAGAAGGATTAGGGAGGGCCATGAAGTGAGTTTGGCTCTCCTTCACGGGGCTGAACTTGATAAATTACTTAAGGTGGCTGTGCAGGAGATCATAGCTGTGGAGATGGACAAGGCCATACGGAGGGCTGGGTTGTTAGACTTGGGACCTTCTTTGGCTGCACTGCTCGGAATGGCAGGCGAAATGAACAGAAATACATCACAGTCGACAAACCAGGTGAATGAAGTGAATGCATCAACCAGTCTTCATGCAGCTACTATTGCTAATCAGGATTGTGTGGCAAAACCTGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

992

Amino Acids

110.89

Weight (kDa)

6.67

Isoelectric Point (pI)

34.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 40 - 187 1.5e-15 Periplasmic binding protein
ANF_receptor PF01094 57 - 187 1.3e-33 Receptor family ligand binding region
ANF_receptor PF01094 188 - 345 1.7e-22 Receptor family ligand binding region
SBP_bac_3 PF00497 424 - 755 5.1e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd PF10613 426 - 516 2.5e-07 Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan PF00060 531 - 788 2e-36 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1239
AatII GACGTC 1 cut(s) 1487
Acc36I ACCTGC 1 cut(s) 1018
AccB1I GGYRCC 1 cut(s) 1619
AccI GTMKAC 1 cut(s) 2888
AciI CCGC 1 cut(s) 563
AclWI GGATC 2 cut(s) 599, 1605
AcsI RAATTY 5 cut(s) 197, 1244, 1439, 1933, 2431
AcuI CTGAAG 1 cut(s) 2289
AcyI GRCGYC 1 cut(s) 1484
AfaI GTAC 9 cut(s) 20, 148, 684, 1162, 1218, 1527, 1809, 1944, 2330
AfiI CCNNNNNNNGG 5 cut(s) 632, 1172, 1193, 2731, 2805
AflII CTTAAG 2 cut(s) 970, 2753
AjnI CCWGG 3 cut(s) 141, 1147, 2895
Alw26I GTCTC 3 cut(s) 429, 2034, 2225
AlwI GGATC 2 cut(s) 599, 1605
AoxI GGCC 5 cut(s) 1145, 2341, 2420, 2701, 2796
ApeKI GCWGC 9 cut(s) 174, 275, 284, 1013, 1322, 1394, 1870, 2840, 2933
ApoI RAATTY 5 cut(s) 197, 1244, 1439, 1933, 2431
Asp700I GAANNNNTTC 4 cut(s) 440, 525, 2090, 2258
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 7 cut(s) 329, 926, 954, 1068, 2129, 2701, 2828
AsuHPI GGTGA 7 cut(s) 95, 1363, 1549, 1774, 1922, 2558, 2911
AvaII GGWCC 6 cut(s) 329, 926, 954, 1068, 2129, 2828
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BanI GGYRCC 1 cut(s) 1619
BarI GAAGNNNNNNTAC 2 cut(s) 1298, 1330
BauI CACGAG 1 cut(s) 2169
BbsI GAAGAC 3 cut(s) 167, 546, 2918
BbvCI CCTCAGC 2 cut(s) 73, 950
BbvI GCAGC 9 cut(s) 161, 287, 296, 1025, 1334, 1381, 1882, 2827, 2945
BccI CCATC 7 cut(s) 83, 494, 596, 714, 1525, 2444, 2782
BceAI ACGGC 1 cut(s) 180
BcgI CGANNNNNNTGC 2 cut(s) 2374, 2408
BciT130I CCWGG 3 cut(s) 143, 1149, 2897
BciVI GTATCC 1 cut(s) 1916
BclI TGATCA 3 cut(s) 994, 1788, 2503
BcoDI GTCTC 3 cut(s) 429, 2034, 2225
BfaI CTAG 6 cut(s) 207, 255, 461, 2538, 2564, 2585
BfmI CTRYAG 8 cut(s) 273, 819, 1011, 1395, 1449, 1857, 1875, 1996
BfoI RGCGCY 1 cut(s) 191
BfrI CTTAAG 2 cut(s) 970, 2753
BfuAI ACCTGC 1 cut(s) 1018
BfuI GTATCC 1 cut(s) 1916
BisI GCNGC 9 cut(s) 175, 276, 285, 1014, 1323, 1395, 1871, 2841, 2934
BlpI GCTNAGC 1 cut(s) 1840
BlsI GCNGC 9 cut(s) 176, 277, 286, 1015, 1324, 1396, 1872, 2842, 2935
BmcAI AGTACT 1 cut(s) 1162
Bme1390I CCNGG 3 cut(s) 143, 1149, 2897
Bme18I GGWCC 6 cut(s) 329, 926, 954, 1068, 2129, 2828
BmgT120I GGNCC 7 cut(s) 329, 926, 954, 1068, 2129, 2701, 2828
BmiI GGNNCC 8 cut(s) 94, 330, 331, 1070, 1265, 1621, 2176, 2829
BmrFI CCNGG 3 cut(s) 143, 1149, 2897
BmrI ACTGGG 1 cut(s) 1623
BmsI GCATC 5 cut(s) 1099, 1435, 2047, 2550, 2924
BmuI ACTGGG 1 cut(s) 1623
BpiI GAAGAC 3 cut(s) 167, 546, 2918
BplI GAGNNNNNCTC 2 cut(s) 2704, 2736
BpmI CTGGAG 1 cut(s) 650
Bpu10I CCTNAGC 3 cut(s) 73, 813, 950
Bpu1102I GCTNAGC 1 cut(s) 1840
BpuEI CTTGAG 3 cut(s) 459, 2016, 2205
BsaAI YACGTR 1 cut(s) 2083
BsaBI GATNNNNATC 2 cut(s) 610, 1113
BsaHI GRCGYC 1 cut(s) 1484
BsaJI CCNNGG 3 cut(s) 142, 183, 929
BsaWI WCCGGW 2 cut(s) 95, 151
BsaXI ACNNNNNCTCC 2 cut(s) 1147, 1177
Bsc4I CCNNNNNNNGG 5 cut(s) 632, 1172, 1193, 2731, 2805
Bse1I ACTGG 7 cut(s) 633, 852, 1158, 1198, 1476, 1618, 2922
Bse3DI GCAATG 1 cut(s) 944
Bse8I GATNNNNATC 2 cut(s) 610, 1113
BseBI CCWGG 3 cut(s) 143, 1149, 2897
BseDI CCNNGG 3 cut(s) 142, 183, 929
BseGI GGATG 5 cut(s) 491, 622, 1536, 1812, 2436
BseJI GATNNNNATC 2 cut(s) 610, 1113
BseLI CCNNNNNNNGG 5 cut(s) 632, 1172, 1193, 2731, 2805
BseMI GCAATG 1 cut(s) 944
BseMII CTCAG 4 cut(s) 87, 941, 1308, 1912
BseNI ACTGG 7 cut(s) 633, 852, 1158, 1198, 1476, 1618, 2922
BseRI GAGGAG 3 cut(s) 61, 1991, 2693
BseXI GCAGC 9 cut(s) 161, 287, 296, 1025, 1334, 1381, 1882, 2827, 2945
BseYI CCCAGC 1 cut(s) 2810
BsgI GTGCAG 2 cut(s) 2786, 2826
BshFI GGCC 5 cut(s) 1147, 2343, 2422, 2703, 2798
BshNI GGYRCC 1 cut(s) 1619
BsiSI CCGG 2 cut(s) 96, 152
BsiWI CGTACG 1 cut(s) 1525
BslFI GGGAC 4 cut(s) 315, 754, 1414, 2841
BslI CCNNNNNNNGG 5 cut(s) 632, 1172, 1193, 2731, 2805
BsmAI GTCTC 3 cut(s) 429, 2034, 2225
BsmFI GGGAC 4 cut(s) 315, 754, 1414, 2841
BsmI GAATGC 1 cut(s) 2917
BsnI GGCC 5 cut(s) 1147, 2343, 2422, 2703, 2798
Bsp1407I TGTACA 1 cut(s) 2328
Bsp143I GATC 8 cut(s) 591, 994, 1411, 1597, 1788, 2224, 2503, 2772
Bsp1720I GCTNAGC 1 cut(s) 1840
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 1 cut(s) 563
BspANI GGCC 5 cut(s) 1147, 2343, 2422, 2703, 2798
BspCNI CTCAG 4 cut(s) 86, 942, 1307, 1911
BspHI TCATGA 2 cut(s) 1035, 2227
BspLI GGNNCC 8 cut(s) 94, 330, 331, 1070, 1265, 1621, 2176, 2829
BspMAI CTGCAG 4 cut(s) 277, 1015, 1399, 1879
BspMI ACCTGC 1 cut(s) 1018
BspPI GGATC 2 cut(s) 599, 1605
BspQI GCTCTTC 1 cut(s) 2096
BspT107I GGYRCC 1 cut(s) 1619
BspTI CTTAAG 2 cut(s) 970, 2753
BsrDI GCAATG 1 cut(s) 944
BsrGI TGTACA 1 cut(s) 2328
BsrI ACTGG 7 cut(s) 633, 852, 1158, 1198, 1476, 1618, 2922
BssECI CCNNGG 3 cut(s) 142, 183, 929
BssMI GATC 8 cut(s) 591, 994, 1411, 1597, 1788, 2224, 2503, 2772
BssNI GRCGYC 1 cut(s) 1484
BssSI CACGAG 1 cut(s) 2169
BssT1I CCWWGG 2 cut(s) 183, 929
Bst2BI CACGAG 1 cut(s) 2169
Bst2UI CCWGG 3 cut(s) 143, 1149, 2897
Bst4CI ACNGT 7 cut(s) 244, 440, 799, 1235, 1858, 2418, 2886
Bst6I CTCTTC 7 cut(s) 303, 475, 2096, 2308, 2660, 2670, 2678
BstACI GRCGYC 1 cut(s) 1484
BstAFI CTTAAG 2 cut(s) 970, 2753
BstAUI TGTACA 1 cut(s) 2328
BstBAI YACGTR 1 cut(s) 2083
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 5 cut(s) 491, 622, 1536, 1812, 2436
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 8 cut(s) 594, 997, 1414, 1600, 1791, 2227, 2506, 2775
BstMAI GTCTC 3 cut(s) 429, 2034, 2225
BstMBI GATC 8 cut(s) 591, 994, 1411, 1597, 1788, 2224, 2503, 2772
BstMWI GCNNNNNNNGC 8 cut(s) 212, 281, 284, 777, 1841, 2331, 2340, 2942
BstNI CCWGG 3 cut(s) 143, 1149, 2897
BstSCI CCNGG 3 cut(s) 141, 1147, 2895
BstSFI CTRYAG 8 cut(s) 273, 819, 1011, 1395, 1449, 1857, 1875, 1996
BstV1I GCAGC 9 cut(s) 161, 287, 296, 1025, 1334, 1381, 1882, 2827, 2945
BstV2I GAAGAC 3 cut(s) 167, 546, 2918
BstX2I RGATCY 1 cut(s) 1597
BstXI CCANNNNNNTGG 1 cut(s) 1176
BstYI RGATCY 1 cut(s) 1597
BsuI GTATCC 1 cut(s) 1916
BsuRI GGCC 5 cut(s) 1147, 2343, 2422, 2703, 2798
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 5 cut(s) 491, 622, 1536, 1812, 2436
BtsI GCAGTG 3 cut(s) 1830, 1847, 2843
BtsIMutI CAGTG 6 cut(s) 626, 859, 1830, 1847, 2423, 2843
BveI ACCTGC 1 cut(s) 1018
CciI TCATGA 2 cut(s) 1035, 2227
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 7 cut(s) 329, 926, 954, 1068, 2129, 2701, 2828
CseI GACGC 1 cut(s) 267
CsiI ACCWGGT 1 cut(s) 2895
Csp6I GTAC 9 cut(s) 19, 147, 683, 1161, 1217, 1526, 1808, 1943, 2329
CspCI CAANNNNNGTGG 2 cut(s) 2029, 2064
CviQI GTAC 9 cut(s) 19, 147, 683, 1161, 1217, 1526, 1808, 1943, 2329
DpnI GATC 8 cut(s) 593, 996, 1413, 1599, 1790, 2226, 2505, 2774
DpnII GATC 8 cut(s) 591, 994, 1411, 1597, 1788, 2224, 2503, 2772
DraI TTTAAA 1 cut(s) 861
Eam1104I CTCTTC 7 cut(s) 303, 475, 2096, 2308, 2660, 2670, 2678
EarI CTCTTC 7 cut(s) 303, 475, 2096, 2308, 2660, 2670, 2678
Eco130I CCWWGG 2 cut(s) 183, 929
Eco147I AGGCCT 1 cut(s) 2343
Eco32I GATATC 2 cut(s) 1312, 2388
Eco47I GGWCC 6 cut(s) 329, 926, 954, 1068, 2129, 2828
Eco57I CTGAAG 1 cut(s) 2289
EcoO109I RGGNCCY 2 cut(s) 329, 2828
EcoRI GAATTC 3 cut(s) 197, 1933, 2431
EcoRII CCWGG 3 cut(s) 141, 1147, 2895
EcoRV GATATC 2 cut(s) 1312, 2388
EcoT14I CCWWGG 2 cut(s) 183, 929
EcoT22I ATGCAT 1 cut(s) 2917
ErhI CCWWGG 2 cut(s) 183, 929
FalI AAGNNNNNCTT 6 cut(s) 403, 435, 513, 545, 1828, 1860
FaqI GGGAC 4 cut(s) 315, 754, 1414, 2841
FauI CCCGC 1 cut(s) 556
FauNDI CATATG 2 cut(s) 508, 1793
FbaI TGATCA 3 cut(s) 994, 1788, 2503
FblI GTMKAC 1 cut(s) 2888
Fnu4HI GCNGC 9 cut(s) 175, 276, 285, 1014, 1323, 1395, 1871, 2841, 2934
FokI GGATG 5 cut(s) 478, 609, 1543, 1799, 2423
Fsp4HI GCNGC 9 cut(s) 175, 276, 285, 1014, 1323, 1395, 1871, 2841, 2934
FspBI CTAG 6 cut(s) 207, 255, 461, 2538, 2564, 2585
GlaI GCGC 1 cut(s) 189
GluI GCNGC 9 cut(s) 175, 276, 285, 1014, 1323, 1395, 1871, 2841, 2934
GsaI CCCAGC 1 cut(s) 2814
GsuI CTGGAG 1 cut(s) 650
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 5 cut(s) 1147, 2343, 2422, 2703, 2798
HapII CCGG 2 cut(s) 96, 152
HgaI GACGC 1 cut(s) 267
HhaI GCGC 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 1484
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HincII GTYRAC 3 cut(s) 693, 1608, 2889
HindII GTYRAC 3 cut(s) 693, 1608, 2889
HindIII AAGCTT 2 cut(s) 1837, 2550
HpaII CCGG 2 cut(s) 96, 152
HphI GGTGA 7 cut(s) 95, 1363, 1549, 1774, 1922, 2558, 2911
Hpy166II GTNNAC 6 cut(s) 693, 1217, 1608, 1688, 2329, 2889
Hpy8I GTNNAC 6 cut(s) 693, 1217, 1608, 1688, 2329, 2889
HpyCH4III ACNGT 7 cut(s) 244, 440, 799, 1235, 1858, 2418, 2886
HpyCH4IV ACGT 3 cut(s) 1484, 1903, 2082
HpyF10VI GCNNNNNNNGC 8 cut(s) 212, 281, 284, 777, 1841, 2331, 2340, 2942
HpySE526I ACGT 3 cut(s) 1484, 1903, 2082
Hsp92I GRCGYC 1 cut(s) 1484
HspAI GCGC 1 cut(s) 188
KflI GGGWCCC 1 cut(s) 329
Ksp22I TGATCA 3 cut(s) 994, 1788, 2503
Kzo9I GATC 8 cut(s) 591, 994, 1411, 1597, 1788, 2224, 2503, 2772
LguI GCTCTTC 1 cut(s) 2096
LmnI GCTCC 1 cut(s) 2180
Lsp1109I GCAGC 9 cut(s) 161, 287, 296, 1025, 1334, 1381, 1882, 2827, 2945
LweI GCATC 5 cut(s) 1099, 1435, 2047, 2550, 2924
MabI ACCWGGT 1 cut(s) 2895
MaeI CTAG 6 cut(s) 207, 255, 461, 2538, 2564, 2585
MaeII ACGT 3 cut(s) 1484, 1903, 2082
MaeIII GTNAC 6 cut(s) 618, 670, 1189, 1222, 1826, 2215
MalI GATC 8 cut(s) 593, 996, 1413, 1599, 1790, 2226, 2505, 2774
MboI GATC 8 cut(s) 591, 994, 1411, 1597, 1788, 2224, 2503, 2772
MfeI CAATTG 1 cut(s) 1768
MflI RGATCY 1 cut(s) 1597
MlyI GAGTC 4 cut(s) 461, 748, 2212, 2576
MmeI TCCRAC 4 cut(s) 452, 1900, 2107, 2621
Mph1103I ATGCAT 1 cut(s) 2917
MroXI GAANNNNTTC 4 cut(s) 440, 525, 2090, 2258
MseI TTAA 9 cut(s) 501, 702, 860, 971, 1131, 1428, 1545, 1955, 2754
MslI CAYNNNNRTG 3 cut(s) 182, 511, 2780
MspA1I CMGCKG 2 cut(s) 77, 1637
MspCI CTTAAG 2 cut(s) 970, 2753
MspI CCGG 2 cut(s) 96, 152
MspR9I CCNGG 3 cut(s) 143, 1149, 2897
MunI CAATTG 1 cut(s) 1768
Mva1269I GAATGC 1 cut(s) 2917
MvaI CCWGG 3 cut(s) 143, 1149, 2897
MwoI GCNNNNNNNGC 8 cut(s) 212, 281, 284, 777, 1841, 2331, 2340, 2942
NcoI CCATGG 1 cut(s) 183
NdeI CATATG 2 cut(s) 508, 1793
NdeII GATC 8 cut(s) 591, 994, 1411, 1597, 1788, 2224, 2503, 2772
NlaIV GGNNCC 8 cut(s) 94, 330, 331, 1070, 1265, 1621, 2176, 2829
NmuCI GTSAC 1 cut(s) 2215
NsiI ATGCAT 1 cut(s) 2917
PagI TCATGA 2 cut(s) 1035, 2227
PceI AGGCCT 1 cut(s) 2343
PciSI GCTCTTC 1 cut(s) 2096
PctI GAATGC 1 cut(s) 2917
PdmI GAANNNNTTC 4 cut(s) 440, 525, 2090, 2258
PfeI GAWTC 8 cut(s) 235, 251, 647, 920, 1082, 1517, 2270, 2654
Pfl23II CGTACG 1 cut(s) 1525
PkrI GCNGC 9 cut(s) 176, 277, 286, 1015, 1324, 1396, 1872, 2842, 2935
PleI GAGTC 4 cut(s) 461, 748, 2212, 2575
PpsI GAGTC 4 cut(s) 461, 748, 2212, 2575
Ppu21I YACGTR 1 cut(s) 2083
PpuMI RGGWCCY 2 cut(s) 329, 2828
PsiI TTATAA 1 cut(s) 1239
Psp5II RGGWCCY 2 cut(s) 329, 2828
Psp6I CCWGG 3 cut(s) 141, 1147, 2895
PspFI CCCAGC 1 cut(s) 2810
PspGI CCWGG 3 cut(s) 141, 1147, 2895
PspLI CGTACG 1 cut(s) 1525
PspN4I GGNNCC 8 cut(s) 94, 330, 331, 1070, 1265, 1621, 2176, 2829
PspPI GGNCC 7 cut(s) 329, 926, 954, 1068, 2129, 2701, 2828
PspPPI RGGWCCY 2 cut(s) 329, 2828
PstI CTGCAG 4 cut(s) 277, 1015, 1399, 1879
PsuI RGATCY 1 cut(s) 1597
PvuII CAGCTG 2 cut(s) 77, 1637
RsaI GTAC 9 cut(s) 20, 148, 684, 1162, 1218, 1527, 1809, 1944, 2330
RsaNI GTAC 9 cut(s) 19, 147, 683, 1161, 1217, 1526, 1808, 1943, 2329
RseI CAYNNNNRTG 3 cut(s) 182, 511, 2780
SalI GTCGAC 1 cut(s) 2887
SapI GCTCTTC 1 cut(s) 2096
SaqAI TTAA 9 cut(s) 501, 702, 860, 971, 1131, 1428, 1545, 1955, 2754
SatI GCNGC 9 cut(s) 175, 276, 285, 1014, 1323, 1395, 1871, 2841, 2934
Sau3AI GATC 8 cut(s) 591, 994, 1411, 1597, 1788, 2224, 2503, 2772
Sau96I GGNCC 7 cut(s) 329, 926, 954, 1068, 2129, 2701, 2828
ScaI AGTACT 1 cut(s) 1162
SchI GAGTC 4 cut(s) 461, 748, 2212, 2576
ScrFI CCNGG 3 cut(s) 143, 1149, 2897
SexAI ACCWGGT 1 cut(s) 2895
SfaNI GCATC 5 cut(s) 1099, 1435, 2047, 2550, 2924
SfcI CTRYAG 8 cut(s) 273, 819, 1011, 1395, 1449, 1857, 1875, 1996
SinI GGWCC 6 cut(s) 329, 926, 954, 1068, 2129, 2828
SmiMI CAYNNNNRTG 3 cut(s) 182, 511, 2780
SmlI CTYRAG 5 cut(s) 474, 970, 2031, 2220, 2753
SmoI CTYRAG 5 cut(s) 474, 970, 2031, 2220, 2753
SseBI AGGCCT 1 cut(s) 2343
SsiI CCGC 1 cut(s) 563
SspI AATATT 2 cut(s) 897, 1026
SspMI CTAG 6 cut(s) 207, 255, 461, 2538, 2564, 2585
StuI AGGCCT 1 cut(s) 2343
StyD4I CCNGG 3 cut(s) 141, 1147, 2895
StyI CCWWGG 2 cut(s) 183, 929
TaaI ACNGT 7 cut(s) 244, 440, 799, 1235, 1858, 2418, 2886
TaiI ACGT 3 cut(s) 1487, 1906, 2085
TaqI TCGA 5 cut(s) 223, 2113, 2198, 2384, 2888
TatI WGTACW 2 cut(s) 1160, 2328
TfiI GAWTC 8 cut(s) 235, 251, 647, 920, 1082, 1517, 2270, 2654
Tru1I TTAA 9 cut(s) 501, 702, 860, 971, 1131, 1428, 1545, 1955, 2754
Tru9I TTAA 9 cut(s) 501, 702, 860, 971, 1131, 1428, 1545, 1955, 2754
TscAI CASTG 6 cut(s) 633, 859, 1837, 1854, 2423, 2850
TseFI GTSAC 1 cut(s) 2215
TseI GCWGC 9 cut(s) 174, 275, 284, 1013, 1322, 1394, 1870, 2840, 2933
Tsp45I GTSAC 1 cut(s) 2215
TspGWI ACGGA 5 cut(s) 1543, 1688, 2163, 2266, 2818
TspRI CASTG 6 cut(s) 633, 859, 1837, 1854, 2423, 2850
Vha464I CTTAAG 2 cut(s) 970, 2753
VpaK11BI GGWCC 6 cut(s) 329, 926, 954, 1068, 2129, 2828
XapI RAATTY 5 cut(s) 197, 1244, 1439, 1933, 2431
XbaI TCTAGA 1 cut(s) 460
XcmI CCANNNNNNNNNTGG 1 cut(s) 1173
XmiI GTMKAC 1 cut(s) 2888
XmnI GAANNNNTTC 4 cut(s) 440, 525, 2090, 2258
XspI CTAG 6 cut(s) 207, 255, 461, 2538, 2564, 2585
ZraI GACGTC 1 cut(s) 1485
ZrmI AGTACT 1 cut(s) 1162
Zsp2I ATGCAT 1 cut(s) 2917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.