Rw4G009970

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006930
Physical Location & Seq
Forward (+)
22716393 .. 22720536
4144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009970.1

Sequence Viewer

Length: 3096 bp
ATGGCAAACAACAATCAGTACCTCCTGATAATCTTCATGTTATTAGATGTTTGCCTAATCTATGGCAACTCCTCAGCTGTTGGTGAAGATGGAACCGGAAGAAAATCACAGCAAATGGTAAATGTGGGCGTAATTCTTGACCTGGGTACTCCGGTTGGGAAGACGGCTCAACGCTGCATATCCATGGCGCTCTCGGAATTCTATGCTAGAAATGCCAACTATCGAACAAAGCTGAATCTTACTGTGAAGGATTCTAGGAATGATGTTGTTTCTGCAGCATCAGCAGCTTTAGAATTGCTAAATAATGAAGAGGTGCAAGCTATTATAGGTCCCCAAACATCAAACCAAGCTAAGTTTGTCATAAGTCTTGGGGAGAAAGCACAAATCCCCATTATCTCGTTTTCAGCCACAAGCCCTTCTTTGTCTCCAACTATGAACCGTTTCTTCATCAGGACAGCTCTGGATGACTCTTCTCAAGTAAAAGCCATCACATCCATTGTTAAAGCATATGGGTGGCATGAAGTTGTTCTTATATATGAAGACACCGAATATGGAAATGGTTTGATTCCCTATATAATTGATGCCTTTCAACAAATTGACACCAGGGTTCCTTACAGAAGTGTCATTTCCCCATCATCCAGTCGCTTAAACATTTTGAGAAAGCTTATCAAGTTAATGGCAATGCAGACGAGGGTATTCTTGGTGCACATGACTCCTTCACTTGGTTCTAAACTCTTTTTACTTGCAAAAGATGCAGGCATGATGAGTGAAGGGTATGCTTGGATCATAACTGATGGATTATCATCTGGGTTACATCCACTGGAGGGAAAGGTCATTGATTCAATGGAAGGTGTGGTGGGCGTAAAGCCCTATGTACCAATGTCAACAGGTCTTAATGATTTTGAGATGAGATGGAAACAAAGCACTATTGGGACTACAAAGCAGACTCATTCTAAGATAAGCATATTTGGCTTATGGGCTTATGATACAGTTTGGGCATTAGCTAAGGCTGTAGAACTCGTTGAGTTGCAGGCAAATTATTCCAGTGCTTTAAAGAACAATAATAAAGGGAAAAACACAGGTGGAATATTGAGCCAAATTGGTGTGTCTAAATCAGGTCCAAGGCTTCTCCAAACATTACTGAGGACCAGATTTGAAGGCTTAAGTGGGAGATTTAGATTGATTGATCAACAATTTCAACCTGCAGCCTTTCAAATATTCAATGTCATGAGTAAGAGGGAGAGAGTGATTAGATATTGGACCCCCAACAAAGAATCAATTGATGATTTAGTTCAATCTGATTATCATCGTGAGTTTATCATTAAAGAACCTACATGGCCTGGAAACACCAGTACTCCACCAAAGGGTTGGGATATACCTATAGCTGGTAAAAAGTTGAGGATTGGTGTACCTGCTGTAACATATGACAGTTTTAGTGAATTTATGAAGATAGAATGGTTTCCTCACACCAATGAGCCTAAGAGCTTCTCAGGGTTCTCGTATGATGTCTTCCGTGCAGCCTTAGAAAGAGTGCCATTTGCACTTCCACACAAGTTCATTCCATTCACCAATGGTTCATCAAGAGAGGCTGCAGGGACTTATGACGATCTTCTTTACCAGATTAAGTTGAATAAATTTGATGCTGTAGTGGGAGATACAACAATCACTGGCAGTAGGACGTCTTATGTTGATTTTACACTACCTTATTTTGAATCAGGCGTACGGATGGTGGTGAAAGTTAAAGATGACCAAAGAAAGAATATGTGGATTTTCCTAAAACCTCTTAGTTGGGATCTTTGGTTGACAACTGGGGGTGCCTTCATCTTTACAGCTGTTGTAATATGGGTCTTGGAACACAATCAAAACAAGGAGTTCAAGGGTTCACGACAGCAACAAATTGGCACAACCCTTTGGTTTTCTTTTTCAACTCTTGTTTTTGCACACAAGGAGAGGGTAGTGAGCAATTGGACAAGATTTGTGCTGATCATATGGATTTTTCTGGTACTCATCCTAACACAGAGTTACACTGCAAGCTTAGCATCACTGCTGACTGTAGAAAGGTTGCAGCCAGCAGTTGTTGATGTAAATGAACTCAGACGTAATGGTGATTATGTGGGATACCAAAAGAATTCCTTTGTACGAGAGCTTTTGATAAGAGAATTGAAGTTTGAGGAGAACAGGCTAAAGTATTATAGATTTCCGGAGGATTATCATAAAGCATTGTCTCAAGGAAGCAAAAATGGTGGTGTTGATGCTTTCTTCGACGAAATCCCCTACTTGAAGCTCTTCCTGGCCAAGTATTATGCTGAATACACTGTTGTTGGACCAACCTACAGAACTGACGGATTTGGCTTTGCCTTCCCACTAGGCTCCCCTCTTGTTGCTTACTTTTCGAGGGCAATCTTGAACGTGACTCAAGATCATGAGACAATGGAACAACTGCAACGGAAGTATTTCCCAGATGAATCTAAATGTCAAGATAAGAGTGCCACATTCTCTTCAGAAAATCCAAGTCTAAGCGTGTACAGCTTTGCAGGCCTGTTCATCATCACAGCAGTCATTTCAATTTCTTCATGCTCGATATGTATACTTTCCAAGTTCCTGTATAAACAGTGGCCTGTATTGAATTCCATCCATTCAGAAGCCAGCTCACTCTTGTCAAAAATATCAAAGCATTTTCATCAAAACAAAGCAACTGATCAACAGAAAACTGCTTCATCTGCAACAAATGCTAGAGATGGTGAAGCTTCACAGATGCTAGAGTCTTTCCACCAAGAACTAGACAATGTTCCAACAGATGAACATAAGGTGCTGTCCAGAGCAGTTAACAGGCATTTAGATGAAAGAGATTCAGAAATGAAGAGGACTGAAGAGGAGCAGAGAAGGATTAGGGAGGGCCATGAAGTGAGTTTGGCTCTTCTTGACAGGGCTGAACTTGATAAATTACTTAAGGTGGCTGTACAGGAGATCATAGCAATGGAGATGGACAAGGCCATGCAGAGGGCTGGATTGTTAGACTTGGGACCTTCTTTGGCTGCACTGCTCGGAATGGCAAACGAACAGAAGTACATCACAGTCGACAAACCAGGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

1031

Amino Acids

116.34

Weight (kDa)

8.58

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 40 - 340 2.4e-23 Periplasmic binding protein
ANF_receptor PF01094 57 - 409 5.3e-80 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 490 - 581 5.1e-08 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 492 - 820 3.4e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 596 - 853 1.3e-37 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1682
Acc36I ACCTGC 2 cut(s) 1210, 1420
AccB1I GGYRCC 1 cut(s) 1814
AccI GTMKAC 2 cut(s) 2587, 3078
AccIII TCCGGA 1 cut(s) 2200
AclWI GGATC 2 cut(s) 791, 1800
AcoI YGGCCR 1 cut(s) 2292
AcsI RAATTY 5 cut(s) 197, 1439, 1634, 2128, 2626
AcuI CTGAAG 2 cut(s) 2484, 2889
AcyI GRCGYC 1 cut(s) 1679
AfiI CCNNNNNNNGG 5 cut(s) 722, 824, 1364, 1385, 3000
AflII CTTAAG 2 cut(s) 1162, 2948
AjnI CCWGG 5 cut(s) 141, 602, 1339, 2289, 3085
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 3 cut(s) 429, 2229, 2420
Alw44I GTGCAC 1 cut(s) 704
AlwI GGATC 2 cut(s) 791, 1800
Aor13HI TCCGGA 1 cut(s) 2200
AoxI GGCC 6 cut(s) 1337, 2292, 2536, 2615, 2896, 2991
ApaLI GTGCAC 1 cut(s) 704
ApeKI GCWGC 8 cut(s) 174, 275, 284, 1205, 1517, 1589, 2065, 3035
ApoI RAATTY 5 cut(s) 197, 1439, 1634, 2128, 2626
Asp700I GAANNNNTTC 5 cut(s) 440, 525, 1458, 2285, 2453
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2324, 2896, 3023
AsuHPI GGTGA 5 cut(s) 95, 1558, 1744, 2117, 2753
AvaII GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2324, 3023
BaeGI GKGCMC 1 cut(s) 708
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BalI TGGCCA 1 cut(s) 2294
BanI GGYRCC 1 cut(s) 1814
BarI GAAGNNNNNNTAC 2 cut(s) 1493, 1525
BbsI GAAGAC 3 cut(s) 167, 546, 1501
Bbv12I GWGCWC 1 cut(s) 708
BbvCI CCTCAGC 1 cut(s) 73
BbvI GCAGC 8 cut(s) 161, 287, 296, 1217, 1529, 1576, 2077, 3022
BccI CCATC 9 cut(s) 83, 494, 640, 788, 906, 1720, 2639, 2732, 2977
BceAI ACGGC 1 cut(s) 180
BciT130I CCWGG 5 cut(s) 143, 604, 1341, 2291, 3087
BciVI GTATCC 1 cut(s) 2111
BclI TGATCA 3 cut(s) 1186, 1983, 2698
BcoDI GTCTC 3 cut(s) 429, 2229, 2420
BfaI CTAG 6 cut(s) 207, 255, 2366, 2733, 2759, 2780
BfmI CTRYAG 8 cut(s) 273, 1011, 1203, 1380, 1590, 1644, 2052, 2332
BfoI RGCGCY 1 cut(s) 191
BfrI CTTAAG 2 cut(s) 1162, 2948
BfuAI ACCTGC 2 cut(s) 1210, 1420
BfuI GTATCC 1 cut(s) 2111
BisI GCNGC 8 cut(s) 175, 276, 285, 1206, 1518, 1590, 2066, 3036
BlpI GCTNAGC 1 cut(s) 2035
BlsI GCNGC 8 cut(s) 176, 277, 286, 1207, 1519, 1591, 2067, 3037
BmcAI AGTACT 1 cut(s) 1354
Bme1390I CCNGG 5 cut(s) 143, 604, 1341, 2291, 3087
Bme18I GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2324, 3023
BmgT120I GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2324, 2896, 3023
BmiI GGNNCC 7 cut(s) 94, 331, 609, 1262, 1816, 2371, 3024
BmrFI CCNGG 5 cut(s) 143, 604, 1341, 2291, 3087
BmrI ACTGGG 1 cut(s) 1818
BmsI GCATC 7 cut(s) 287, 571, 742, 1630, 2048, 2242, 2745
BmuI ACTGGG 1 cut(s) 1818
BpiI GAAGAC 3 cut(s) 167, 546, 1501
BplI GAGNNNNNCTC 2 cut(s) 2899, 2931
BpmI CTGGAG 1 cut(s) 842
Bpu10I CCTNAGC 2 cut(s) 73, 1005
Bpu1102I GCTNAGC 1 cut(s) 2035
BpuEI CTTGAG 3 cut(s) 459, 2211, 2400
BsaBI GATNNNNATC 2 cut(s) 802, 1305
BsaHI GRCGYC 1 cut(s) 1679
BsaJI CCNNGG 4 cut(s) 142, 183, 603, 1121
BsaWI WCCGGW 3 cut(s) 95, 151, 2200
BsaXI ACNNNNNCTCC 2 cut(s) 1339, 1369
Bsc4I CCNNNNNNNGG 5 cut(s) 722, 824, 1364, 1385, 3000
Bse1I ACTGG 6 cut(s) 639, 825, 1044, 1350, 1672, 1813
Bse3DI GCAATG 2 cut(s) 687, 2982
Bse8I GATNNNNATC 2 cut(s) 802, 1305
BseAI TCCGGA 1 cut(s) 2200
BseBI CCWGG 5 cut(s) 143, 604, 1341, 2291, 3087
BseDI CCNNGG 4 cut(s) 142, 183, 603, 1121
BseGI GGATG 7 cut(s) 469, 491, 635, 814, 1731, 2007, 2631
BseJI GATNNNNATC 2 cut(s) 802, 1305
BseLI CCNNNNNNNGG 5 cut(s) 722, 824, 1364, 1385, 3000
BseMI GCAATG 2 cut(s) 687, 2982
BseMII CTCAG 4 cut(s) 87, 1133, 1503, 2107
BseNI ACTGG 6 cut(s) 639, 825, 1044, 1350, 1672, 1813
BseRI GAGGAG 3 cut(s) 61, 2186, 2888
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 8 cut(s) 161, 287, 296, 1217, 1529, 1576, 2077, 3022
BsgI GTGCAG 2 cut(s) 1536, 3021
BshFI GGCC 6 cut(s) 1339, 2294, 2538, 2617, 2898, 2993
BshNI GGYRCC 1 cut(s) 1814
BsiHKAI GWGCWC 1 cut(s) 708
BsiSI CCGG 3 cut(s) 96, 152, 2201
BsiWI CGTACG 1 cut(s) 1720
BslFI GGGAC 4 cut(s) 315, 946, 1609, 3036
BslI CCNNNNNNNGG 5 cut(s) 722, 824, 1364, 1385, 3000
BsmAI GTCTC 3 cut(s) 429, 2229, 2420
BsmFI GGGAC 4 cut(s) 315, 946, 1609, 3036
BsnI GGCC 6 cut(s) 1339, 2294, 2538, 2617, 2898, 2993
Bsp1286I GDGCHC 1 cut(s) 708
Bsp13I TCCGGA 1 cut(s) 2200
Bsp1407I TGTACA 2 cut(s) 2523, 2959
Bsp143I GATC 8 cut(s) 783, 1186, 1606, 1792, 1983, 2419, 2698, 2967
Bsp1720I GCTNAGC 1 cut(s) 2035
Bsp19I CCATGG 1 cut(s) 183
BspANI GGCC 6 cut(s) 1339, 2294, 2538, 2617, 2898, 2993
BspCNI CTCAG 4 cut(s) 86, 1134, 1502, 2106
BspEI TCCGGA 1 cut(s) 2200
BspHI TCATGA 2 cut(s) 1227, 2422
BspLI GGNNCC 7 cut(s) 94, 331, 609, 1262, 1816, 2371, 3024
BspMAI CTGCAG 3 cut(s) 277, 1207, 1594
BspMI ACCTGC 2 cut(s) 1210, 1420
BspPI GGATC 2 cut(s) 791, 1800
BspQI GCTCTTC 2 cut(s) 2291, 2922
BspT107I GGYRCC 1 cut(s) 1814
BspTI CTTAAG 2 cut(s) 1162, 2948
BsrDI GCAATG 2 cut(s) 687, 2982
BsrGI TGTACA 2 cut(s) 2523, 2959
BsrI ACTGG 6 cut(s) 639, 825, 1044, 1350, 1672, 1813
BssECI CCNNGG 4 cut(s) 142, 183, 603, 1121
BssMI GATC 8 cut(s) 783, 1186, 1606, 1792, 1983, 2419, 2698, 2967
BssNAI GTATAC 1 cut(s) 2588
BssNI GRCGYC 1 cut(s) 1679
BssT1I CCWWGG 2 cut(s) 183, 1121
Bst1107I GTATAC 1 cut(s) 2588
Bst2UI CCWGG 5 cut(s) 143, 604, 1341, 2291, 3087
Bst4CI ACNGT 8 cut(s) 244, 440, 991, 1430, 2053, 2317, 2613, 3076
Bst6I CTCTTC 7 cut(s) 303, 475, 2291, 2503, 2855, 2865, 2922
BstACI GRCGYC 1 cut(s) 1679
BstAFI CTTAAG 2 cut(s) 1162, 2948
BstAPI GCANNNNNTGC 2 cut(s) 752, 2729
BstAUI TGTACA 2 cut(s) 2523, 2959
BstC8I GCNNGC 7 cut(s) 318, 757, 1032, 2032, 2070, 2536, 2647
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 7 cut(s) 469, 491, 635, 814, 1731, 2007, 2631
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 8 cut(s) 786, 1189, 1609, 1795, 1986, 2422, 2701, 2970
BstMAI GTCTC 3 cut(s) 429, 2229, 2420
BstMBI GATC 8 cut(s) 783, 1186, 1606, 1792, 1983, 2419, 2698, 2967
BstNI CCWGG 5 cut(s) 143, 604, 1341, 2291, 3087
BstSCI CCNGG 5 cut(s) 141, 602, 1339, 2289, 3085
BstSFI CTRYAG 8 cut(s) 273, 1011, 1203, 1380, 1590, 1644, 2052, 2332
BstSLI GKGCMC 1 cut(s) 708
BstV1I GCAGC 8 cut(s) 161, 287, 296, 1217, 1529, 1576, 2077, 3022
BstV2I GAAGAC 3 cut(s) 167, 546, 1501
BstX2I RGATCY 1 cut(s) 1792
BstXI CCANNNNNNTGG 1 cut(s) 1368
BstYI RGATCY 1 cut(s) 1792
BstZ17I GTATAC 1 cut(s) 2588
BsuI GTATCC 1 cut(s) 2111
BsuRI GGCC 6 cut(s) 1339, 2294, 2538, 2617, 2898, 2993
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 7 cut(s) 469, 491, 635, 814, 1731, 2007, 2631
BtsI GCAGTG 3 cut(s) 2025, 2042, 3038
BtsIMutI CAGTG 8 cut(s) 818, 1051, 1665, 2025, 2042, 2313, 2618, 3038
BveI ACCTGC 2 cut(s) 1210, 1420
Cac8I GCNNGC 7 cut(s) 318, 757, 1032, 2032, 2070, 2536, 2647
CciI TCATGA 2 cut(s) 1227, 2422
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2324, 2896, 3023
CsiI ACCWGGT 1 cut(s) 3085
CspCI CAANNNNNGTGG 2 cut(s) 2224, 2259
DpnI GATC 8 cut(s) 785, 1188, 1608, 1794, 1985, 2421, 2700, 2969
DpnII GATC 8 cut(s) 783, 1186, 1606, 1792, 1983, 2419, 2698, 2967
DraI TTTAAA 1 cut(s) 1053
EaeI YGGCCR 1 cut(s) 2292
Eam1104I CTCTTC 7 cut(s) 303, 475, 2291, 2503, 2855, 2865, 2922
EarI CTCTTC 7 cut(s) 303, 475, 2291, 2503, 2855, 2865, 2922
Eco130I CCWWGG 2 cut(s) 183, 1121
Eco147I AGGCCT 1 cut(s) 2538
Eco47I GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2324, 3023
Eco57I CTGAAG 2 cut(s) 2484, 2889
EcoO109I RGGNCCY 2 cut(s) 329, 3023
EcoRI GAATTC 3 cut(s) 197, 2128, 2626
EcoRII CCWGG 5 cut(s) 141, 602, 1339, 2289, 3085
EcoT14I CCWWGG 2 cut(s) 183, 1121
ErhI CCWWGG 2 cut(s) 183, 1121
FalI AAGNNNNNCTT 6 cut(s) 403, 435, 513, 545, 2117, 2149
FaqI GGGAC 4 cut(s) 315, 946, 1609, 3036
FauNDI CATATG 3 cut(s) 508, 1423, 1988
FbaI TGATCA 3 cut(s) 1186, 1983, 2698
FblI GTMKAC 2 cut(s) 2587, 3078
Fnu4HI GCNGC 8 cut(s) 175, 276, 285, 1206, 1518, 1590, 2066, 3036
FokI GGATG 7 cut(s) 476, 478, 622, 801, 1738, 1994, 2618
Fsp4HI GCNGC 8 cut(s) 175, 276, 285, 1206, 1518, 1590, 2066, 3036
FspBI CTAG 6 cut(s) 207, 255, 2366, 2733, 2759, 2780
GlaI GCGC 1 cut(s) 189
GluI GCNGC 8 cut(s) 175, 276, 285, 1206, 1518, 1590, 2066, 3036
GsuI CTGGAG 1 cut(s) 842
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 6 cut(s) 1339, 2294, 2538, 2617, 2898, 2993
HapII CCGG 3 cut(s) 96, 152, 2201
HhaI GCGC 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 1679
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HincII GTYRAC 4 cut(s) 885, 1803, 2827, 3079
HindII GTYRAC 4 cut(s) 885, 1803, 2827, 3079
HindIII AAGCTT 3 cut(s) 662, 2032, 2745
HpaI GTTAAC 1 cut(s) 2827
HpaII CCGG 3 cut(s) 96, 152, 2201
HphI GGTGA 5 cut(s) 95, 1558, 1744, 2117, 2753
Hpy166II GTNNAC 9 cut(s) 706, 885, 1409, 1803, 1883, 2524, 2588, 2827, 3079
Hpy188I TCNGA 7 cut(s) 196, 1300, 2096, 2503, 2641, 2854, 3047
Hpy8I GTNNAC 9 cut(s) 706, 885, 1409, 1803, 1883, 2524, 2588, 2827, 3079
Hpy99I CGWCG 1 cut(s) 2267
HpyCH4III ACNGT 8 cut(s) 244, 440, 991, 1430, 2053, 2317, 2613, 3076
HpyCH4IV ACGT 3 cut(s) 1679, 2098, 2409
HpySE526I ACGT 3 cut(s) 1679, 2098, 2409
Hsp92I GRCGYC 1 cut(s) 1679
HspAI GCGC 1 cut(s) 188
Kpn2I TCCGGA 1 cut(s) 2200
Ksp22I TGATCA 3 cut(s) 1186, 1983, 2698
KspAI GTTAAC 1 cut(s) 2827
Kzo9I GATC 8 cut(s) 783, 1186, 1606, 1792, 1983, 2419, 2698, 2967
LguI GCTCTTC 2 cut(s) 2291, 2922
LmnI GCTCC 2 cut(s) 2375, 2875
Lsp1109I GCAGC 8 cut(s) 161, 287, 296, 1217, 1529, 1576, 2077, 3022
LweI GCATC 7 cut(s) 287, 571, 742, 1630, 2048, 2242, 2745
MabI ACCWGGT 1 cut(s) 3085
MaeI CTAG 6 cut(s) 207, 255, 2366, 2733, 2759, 2780
MaeII ACGT 3 cut(s) 1679, 2098, 2409
MaeIII GTNAC 4 cut(s) 810, 1417, 2021, 2410
MalI GATC 8 cut(s) 785, 1188, 1608, 1794, 1985, 2421, 2700, 2969
MboI GATC 8 cut(s) 783, 1186, 1606, 1792, 1983, 2419, 2698, 2967
MfeI CAATTG 2 cut(s) 1278, 1963
MflI RGATCY 1 cut(s) 1792
MhlI GDGCHC 1 cut(s) 708
MlsI TGGCCA 1 cut(s) 2294
MluNI TGGCCA 1 cut(s) 2294
MlyI GAGTC 5 cut(s) 461, 706, 940, 2407, 2771
MmeI TCCRAC 3 cut(s) 452, 2302, 2816
Mox20I TGGCCA 1 cut(s) 2294
MroI TCCGGA 1 cut(s) 2200
MroXI GAANNNNTTC 5 cut(s) 440, 525, 1458, 2285, 2453
MscI TGGCCA 1 cut(s) 2294
MslI CAYNNNNRTG 5 cut(s) 182, 511, 1470, 2838, 2975
Msp20I TGGCCA 1 cut(s) 2294
MspA1I CMGCKG 2 cut(s) 77, 1832
MspCI CTTAAG 2 cut(s) 1162, 2948
MspI CCGG 3 cut(s) 96, 152, 2201
MspR9I CCNGG 5 cut(s) 143, 604, 1341, 2291, 3087
MunI CAATTG 2 cut(s) 1278, 1963
MvaI CCWGG 5 cut(s) 143, 604, 1341, 2291, 3087
NcoI CCATGG 1 cut(s) 183
NdeI CATATG 3 cut(s) 508, 1423, 1988
NdeII GATC 8 cut(s) 783, 1186, 1606, 1792, 1983, 2419, 2698, 2967
NlaIV GGNNCC 7 cut(s) 94, 331, 609, 1262, 1816, 2371, 3024
NmuCI GTSAC 1 cut(s) 2410
PagI TCATGA 2 cut(s) 1227, 2422
PceI AGGCCT 1 cut(s) 2538
PciSI GCTCTTC 2 cut(s) 2291, 2922
PdmI GAANNNNTTC 5 cut(s) 440, 525, 1458, 2285, 2453
PfeI GAWTC 8 cut(s) 235, 251, 565, 839, 1274, 1712, 2465, 2849
Pfl23II CGTACG 1 cut(s) 1720
PkrI GCNGC 8 cut(s) 176, 277, 286, 1207, 1519, 1591, 2067, 3037
PleI GAGTC 5 cut(s) 461, 706, 940, 2407, 2770
PpsI GAGTC 5 cut(s) 461, 706, 940, 2407, 2770
PpuMI RGGWCCY 2 cut(s) 329, 3023
Psp5II RGGWCCY 2 cut(s) 329, 3023
Psp6I CCWGG 5 cut(s) 141, 602, 1339, 2289, 3085
PspGI CCWGG 5 cut(s) 141, 602, 1339, 2289, 3085
PspLI CGTACG 1 cut(s) 1720
PspN4I GGNNCC 7 cut(s) 94, 331, 609, 1262, 1816, 2371, 3024
PspPI GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2324, 2896, 3023
PspPPI RGGWCCY 2 cut(s) 329, 3023
PstI CTGCAG 3 cut(s) 277, 1207, 1594
PsuI RGATCY 1 cut(s) 1792
PvuII CAGCTG 2 cut(s) 77, 1832
RseI CAYNNNNRTG 5 cut(s) 182, 511, 1470, 2838, 2975
SalI GTCGAC 1 cut(s) 3077
SapI GCTCTTC 2 cut(s) 2291, 2922
SatI GCNGC 8 cut(s) 175, 276, 285, 1206, 1518, 1590, 2066, 3036
Sau3AI GATC 8 cut(s) 783, 1186, 1606, 1792, 1983, 2419, 2698, 2967
Sau96I GGNCC 7 cut(s) 329, 1118, 1146, 1260, 2324, 2896, 3023
ScaI AGTACT 1 cut(s) 1354
SchI GAGTC 5 cut(s) 461, 706, 940, 2407, 2771
ScrFI CCNGG 5 cut(s) 143, 604, 1341, 2291, 3087
SduI GDGCHC 1 cut(s) 708
SexAI ACCWGGT 1 cut(s) 3085
SfaNI GCATC 7 cut(s) 287, 571, 742, 1630, 2048, 2242, 2745
SfcI CTRYAG 8 cut(s) 273, 1011, 1203, 1380, 1590, 1644, 2052, 2332
SinI GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2324, 3023
SmiMI CAYNNNNRTG 5 cut(s) 182, 511, 1470, 2838, 2975
SmlI CTYRAG 5 cut(s) 474, 1162, 2226, 2415, 2948
SmoI CTYRAG 5 cut(s) 474, 1162, 2226, 2415, 2948
SseBI AGGCCT 1 cut(s) 2538
SspI AATATT 2 cut(s) 1089, 1218
SspMI CTAG 6 cut(s) 207, 255, 2366, 2733, 2759, 2780
StuI AGGCCT 1 cut(s) 2538
StyD4I CCNGG 5 cut(s) 141, 602, 1339, 2289, 3085
StyI CCWWGG 2 cut(s) 183, 1121
TaaI ACNGT 8 cut(s) 244, 440, 991, 1430, 2053, 2317, 2613, 3076
TaiI ACGT 3 cut(s) 1682, 2101, 2412
TaqI TCGA 5 cut(s) 223, 2262, 2393, 2579, 3078
TatI WGTACW 4 cut(s) 1352, 2523, 2959, 3066
TfiI GAWTC 8 cut(s) 235, 251, 565, 839, 1274, 1712, 2465, 2849
TscAI CASTG 8 cut(s) 825, 1051, 1672, 2032, 2049, 2320, 2618, 3045
TseFI GTSAC 1 cut(s) 2410
TseI GCWGC 8 cut(s) 174, 275, 284, 1205, 1517, 1589, 2065, 3035
Tsp45I GTSAC 1 cut(s) 2410
TspGWI ACGGA 4 cut(s) 1502, 1738, 2358, 2461
TspRI CASTG 8 cut(s) 825, 1051, 1672, 2032, 2049, 2320, 2618, 3045
Vha464I CTTAAG 2 cut(s) 1162, 2948
VneI GTGCAC 1 cut(s) 704
VpaK11BI GGWCC 6 cut(s) 329, 1118, 1146, 1260, 2324, 3023
XapI RAATTY 5 cut(s) 197, 1439, 1634, 2128, 2626
XcmI CCANNNNNNNNNTGG 1 cut(s) 1365
XmiI GTMKAC 2 cut(s) 2587, 3078
XmnI GAANNNNTTC 5 cut(s) 440, 525, 1458, 2285, 2453
XspI CTAG 6 cut(s) 207, 255, 2366, 2733, 2759, 2780
ZraI GACGTC 1 cut(s) 1680
ZrmI AGTACT 1 cut(s) 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.