FvH4_5g02370

AMMECR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004168
Physical Location & Seq
Forward (+)
1449018 .. 1452233
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02370.t2

Sequence Viewer

Length: 633 bp
ATGGTGTCTGCGAGCAAGGAGATGGTGGTTTATTGCTTTGACACTCTCCTGGCTCACTATAATGGTGAAGAAGCTCCTCCCCCAGCCTTTGAGGATGGCCAACATCCATTGTTTGTCACTTGGAAGAAAGTGGTGAATGGTAGTGAGCCTCGTTTGCGTGGATGTATTGGAATACTAGAAGCTCGTGGCTTGGTTAGTGGAATCAAGGACTATACTCTAAACAGTGCTTTGAGGGACCGGAGGTTTTCCCCAATACAAGCTAAAGAATTACCATATTTGGAATGTACAGTTTCCATTCTGACTGATTATGAAACTGCTGACAACTACCTTGATTGGGAGGTTGGGAAACATGGTATAATTATCGAGTTTTCTGATCCAGACTATGGTACAAGGCGTAATGCAACGTACTTGCCTGAGGTCGCTGCCCATGAAGGTTGGACTAACATAGAAGCAATTGACTCATTGATGCGTAAAGCGGGCTATTCTGGGACCATCACAGAGTCTCTTCGGAAGCGTATACGATTGACCCGCTACCAGAGTTCATTATATACAATGCACTACAGTGAATACGTTTCTTACGTGAAGACCACCAGAAGTGCAGTTGCATCTGGCTCCAAGTCCGGTAATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0044424 GO:0044444 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMMECR1

Protein Analysis

211

Amino Acids

23.65

Weight (kDa)

6.22

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMMECR1 PF01871 11 - 181 5.7e-44 AMMECR1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 383
AccI GTMKAC 1 cut(s) 517
AciI CCGC 2 cut(s) 476, 529
AclWI GGATC 1 cut(s) 368
AcoI YGGCCR 1 cut(s) 97
AfaI GTAC 3 cut(s) 286, 388, 407
AfiI CCNNNNNNNGG 2 cut(s) 334, 383
AjnI CCWGG 1 cut(s) 48
AleI CACNNNNGTG 1 cut(s) 561
AluBI AGCT 3 cut(s) 74, 182, 260
AluI AGCT 3 cut(s) 74, 182, 260
Alw26I GTCTC 1 cut(s) 507
AlwI GGATC 1 cut(s) 368
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 1 cut(s) 422
AspS9I GGNCC 2 cut(s) 235, 489
AsuHPI GGTGA 2 cut(s) 77, 145
AvaII GGWCC 2 cut(s) 235, 489
AxyI CCTNAGG 1 cut(s) 414
BalI TGGCCA 1 cut(s) 99
BauI CACGAG 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 590
BbvI GCAGC 1 cut(s) 409
BccI CCATC 3 cut(s) 16, 89, 500
BciT130I CCWGG 1 cut(s) 50
BcoDI GTCTC 1 cut(s) 507
BfaI CTAG 1 cut(s) 176
BfmI CTRYAG 1 cut(s) 559
BisI GCNGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 424
Bme1390I CCNGG 1 cut(s) 50
Bme18I GGWCC 2 cut(s) 235, 489
BmgT120I GGNCC 2 cut(s) 235, 489
BmiI GGNNCC 3 cut(s) 236, 490, 613
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 2 cut(s) 456, 614
BpiI GAAGAC 1 cut(s) 590
BsaAI YACGTR 1 cut(s) 580
BsaWI WCCGGW 2 cut(s) 237, 620
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 383
Bse21I CCTNAGG 1 cut(s) 414
BseBI CCWGG 1 cut(s) 50
BseGI GGATG 3 cut(s) 100, 103, 167
BseLI CCNNNNNNNGG 2 cut(s) 334, 383
BseMII CTCAG 1 cut(s) 405
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 1 cut(s) 409
BseYI CCCAGC 1 cut(s) 82
BsgI GTGCAG 1 cut(s) 618
BshFI GGCC 1 cut(s) 99
BsiSI CCGG 2 cut(s) 238, 621
BslFI GGGAC 2 cut(s) 248, 502
BslI CCNNNNNNNGG 2 cut(s) 334, 383
BsmAI GTCTC 1 cut(s) 507
BsmFI GGGAC 2 cut(s) 248, 502
BsnI GGCC 1 cut(s) 99
Bsp1407I TGTACA 1 cut(s) 284
Bsp143I GATC 1 cut(s) 373
BspACI CCGC 2 cut(s) 476, 529
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 1 cut(s) 406
BspLI GGNNCC 3 cut(s) 236, 490, 613
BspPI GGATC 1 cut(s) 368
BsrGI TGTACA 1 cut(s) 284
BssMI GATC 1 cut(s) 373
BssNAI GTATAC 1 cut(s) 518
BssSI CACGAG 1 cut(s) 183
Bst1107I GTATAC 1 cut(s) 518
Bst2BI CACGAG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 3 cut(s) 224, 289, 563
Bst6I CTCTTC 1 cut(s) 510
BstAUI TGTACA 1 cut(s) 284
BstBAI YACGTR 1 cut(s) 580
BstC8I GCNNGC 2 cut(s) 13, 478
BstDEI CTNAG 1 cut(s) 414
BstF5I GGATG 3 cut(s) 100, 103, 167
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 1 cut(s) 507
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 48
BstSFI CTRYAG 1 cut(s) 559
BstV1I GCAGC 1 cut(s) 409
BstV2I GAAGAC 1 cut(s) 590
BstZ17I GTATAC 1 cut(s) 518
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 1 cut(s) 99
BtsCI GGATG 3 cut(s) 100, 103, 167
BtsIMutI CAGTG 2 cut(s) 229, 568
Cac8I GCNNGC 2 cut(s) 13, 478
Cfr13I GGNCC 2 cut(s) 235, 489
Csp6I GTAC 3 cut(s) 285, 387, 406
CviAII CATG 2 cut(s) 350, 428
CviQI GTAC 3 cut(s) 285, 387, 406
DdeI CTNAG 1 cut(s) 414
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Eco47I GGWCC 2 cut(s) 235, 489
Eco81I CCTNAGG 1 cut(s) 414
EcoRII CCWGG 1 cut(s) 48
FaeI CATG 2 cut(s) 353, 431
FaqI GGGAC 2 cut(s) 248, 502
FatI CATG 2 cut(s) 349, 427
FauI CCCGC 2 cut(s) 469, 536
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 1 cut(s) 423
FokI GGATG 3 cut(s) 90, 107, 174
Fsp4HI GCNGC 1 cut(s) 423
FspBI CTAG 1 cut(s) 176
GluI GCNGC 1 cut(s) 423
GsaI CCCAGC 1 cut(s) 86
HaeIII GGCC 1 cut(s) 99
HapII CCGG 2 cut(s) 238, 621
Hin1II CATG 2 cut(s) 353, 431
HinfI GANTC 3 cut(s) 201, 458, 500
HpaII CCGG 2 cut(s) 238, 621
HphI GGTGA 2 cut(s) 77, 145
Hpy166II GTNNAC 1 cut(s) 518
Hpy188I TCNGA 3 cut(s) 300, 373, 510
Hpy188III TCNNGA 1 cut(s) 377
Hpy8I GTNNAC 1 cut(s) 518
HpyAV CCTTC 1 cut(s) 425
HpyCH4III ACNGT 3 cut(s) 224, 289, 563
HpyCH4IV ACGT 3 cut(s) 404, 570, 579
HpyCH4V TGCA 4 cut(s) 401, 556, 599, 605
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
HpyF3I CTNAG 1 cut(s) 414
HpySE526I ACGT 3 cut(s) 404, 570, 579
Hsp92II CATG 2 cut(s) 353, 431
Kzo9I GATC 1 cut(s) 373
LmnI GCTCC 2 cut(s) 79, 617
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 2 cut(s) 456, 614
MaeI CTAG 1 cut(s) 176
MaeII ACGT 3 cut(s) 404, 570, 579
MaeIII GTNAC 1 cut(s) 115
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 4 cut(s) 80, 136, 497, 595
MfeI CAATTG 1 cut(s) 453
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 3 cut(s) 266, 357, 453
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 2 cut(s) 452, 509
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 7 cut(s) 85, 87, 159, 225, 234, 331, 409
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MslI CAYNNNNRTG 2 cut(s) 60, 561
Msp20I TGGCCA 1 cut(s) 99
MspI CCGG 2 cut(s) 238, 621
MspR9I CCNGG 1 cut(s) 50
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 1 cut(s) 154
NdeII GATC 1 cut(s) 373
NlaIII CATG 2 cut(s) 353, 431
NlaIV GGNNCC 3 cut(s) 236, 490, 613
NmuCI GTSAC 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 576
PfeI GAWTC 1 cut(s) 201
PflMI CCANNNNNTGG 1 cut(s) 383
PkrI GCNGC 1 cut(s) 424
PleI GAGTC 2 cut(s) 452, 508
PpsI GAGTC 2 cut(s) 452, 508
Ppu21I YACGTR 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 48
PspFI CCCAGC 1 cut(s) 82
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 3 cut(s) 236, 490, 613
PspPI GGNCC 2 cut(s) 235, 489
RsaI GTAC 3 cut(s) 286, 388, 407
RsaNI GTAC 3 cut(s) 285, 387, 406
RseI CAYNNNNRTG 2 cut(s) 60, 561
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 2 cut(s) 235, 489
SchI GAGTC 2 cut(s) 452, 509
ScrFI CCNGG 1 cut(s) 50
SfaNI GCATC 2 cut(s) 456, 614
SfcI CTRYAG 1 cut(s) 559
SinI GGWCC 2 cut(s) 235, 489
SmiMI CAYNNNNRTG 2 cut(s) 60, 561
Sse9I AATT 3 cut(s) 266, 357, 453
SsiI CCGC 2 cut(s) 476, 529
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 3 cut(s) 224, 289, 563
TaiI ACGT 3 cut(s) 407, 573, 582
TaqI TCGA 1 cut(s) 363
TasI AATT 3 cut(s) 266, 357, 453
TatI WGTACW 1 cut(s) 284
TfiI GAWTC 1 cut(s) 201
TscAI CASTG 2 cut(s) 229, 568
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 3 cut(s) 324, 444, 531
TspRI CASTG 2 cut(s) 229, 568
Van91I CCANNNNNTGG 1 cut(s) 383
VpaK11BI GGWCC 2 cut(s) 235, 489
XmiI GTMKAC 1 cut(s) 517
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.