MD07G1058400.v1.1

AMMECR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004168
Physical Location & Seq
Forward (+)
5422216 .. 5428234
6019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1058400.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGTGTCGGCGGACAAGGACATGGTGGCTTACTGCTTCGATACTCTCCTCGCTCACTACAGCATCGAACAACCTGCTCCTCCAGCCTTCGACGACGGCCAGCATCCATTGTTCGTTACTTGGAAGAAAGTGGTGAATGGTGGTGAGCCTCGTTTGCGTGGATGCATAGGAACACTAGAACCTCGGGGCTTGATTAATGGATTCAGGGATTACGCTCTAACCAGTGCTTTGAAGGACCGGAGGTTTTCCCCAATACAAGCTAAGGAAATACCTCAGTTGGAATGCACAGTTTCCATTCTGACGGATTATGAAACTGCTAGTCACTACCTTGATTGGGAGATTGGGAAACATGGTATAATTATTGAATTTACTGATCCTGACTATAATACAAGGCGCAGTGCCACATACTTGCCTGAGGTTGCTGCTCATGAAGGTTGGACAAAAACAGAAGCAATTGACTCATTGATGCGGAAATCTGGATATAATGGCAGCATCACTGAGCCGCTTCGGAAGAGCATACGATTGACCCGCTACCAGAGCTCATTATGCACCATGGACCACAACGACTATGTTTCCTACATCAAGGAAACCAGAGGCAGAGCTCCGTCTCTTGTTGGCGCTATATCTGGCAGTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0044424 GO:0044444 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMMECR1

Protein Analysis

213

Amino Acids

23.84

Weight (kDa)

6.31

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMMECR1 PF01871 10 - 181 3.9e-44 AMMECR1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 82
AciI CCGC 4 cut(s) 11, 469, 503, 529
AclWI GGATC 1 cut(s) 368
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 1 cut(s) 365
AfiI CCNNNNNNNGG 1 cut(s) 334
AgsI TTSAA 2 cut(s) 232, 365
AluBI AGCT 3 cut(s) 260, 540, 602
AluI AGCT 3 cut(s) 260, 540, 602
Alw21I GWGCWC 2 cut(s) 542, 604
Alw26I GTCTC 1 cut(s) 612
AlwI GGATC 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 183
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 2 cut(s) 422, 489
ApoI RAATTY 1 cut(s) 365
AseI ATTAAT 1 cut(s) 195
AspLEI GCGC 2 cut(s) 396, 620
AspS9I GGNCC 2 cut(s) 235, 556
AsuHPI GGTGA 2 cut(s) 145, 155
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 2 cut(s) 235, 556
AxyI CCTNAGG 1 cut(s) 414
BanII GRGCYC 2 cut(s) 542, 604
Bbv12I GWGCWC 2 cut(s) 542, 604
BbvI GCAGC 2 cut(s) 409, 501
BceAI ACGGC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 2 cut(s) 176, 318
BfmI CTRYAG 1 cut(s) 58
BfoI RGCGCY 1 cut(s) 621
BfuAI ACCTGC 1 cut(s) 82
BisI GCNGC 3 cut(s) 423, 490, 503
BlsI GCNGC 3 cut(s) 424, 491, 504
Bme18I GGWCC 2 cut(s) 235, 556
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 235, 556
BmsI GCATC 5 cut(s) 72, 112, 152, 456, 501
BpmI CTGGAG 1 cut(s) 66
Bpu10I CCTNAGC 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 182, 552
BsaWI WCCGGW 1 cut(s) 237
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse1I ACTGG 1 cut(s) 222
Bse21I CCTNAGG 1 cut(s) 414
BseDI CCNNGG 2 cut(s) 182, 552
BseGI GGATG 2 cut(s) 103, 167
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMII CTCAG 3 cut(s) 287, 405, 489
BseNI ACTGG 1 cut(s) 222
BseRI GAGGAG 2 cut(s) 38, 69
BseXI GCAGC 2 cut(s) 409, 501
BshFI GGCC 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 542, 604
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 1 cut(s) 238
BslI CCNNNNNNNGG 1 cut(s) 334
BsmAI GTCTC 1 cut(s) 612
BsmBI CGTCTC 1 cut(s) 612
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 1 cut(s) 99
BsoBI CYCGRG 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 542, 604
Bsp143I GATC 1 cut(s) 373
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 4 cut(s) 11, 469, 503, 529
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 3 cut(s) 286, 406, 490
BspHI TCATGA 1 cut(s) 427
BspMI ACCTGC 1 cut(s) 82
BspPI GGATC 1 cut(s) 368
BspQI GCTCTTC 1 cut(s) 506
BsrI ACTGG 1 cut(s) 222
BssECI CCNNGG 2 cut(s) 182, 552
BssMI GATC 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 552
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 506
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 4 cut(s) 261, 273, 414, 498
BstDSI CCRYGG 1 cut(s) 552
BstF5I GGATG 2 cut(s) 103, 167
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 2 cut(s) 396, 620
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 4 cut(s) 83, 154, 537, 546
BstSFI CTRYAG 1 cut(s) 58
BstV1I GCAGC 2 cut(s) 409, 501
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 1 cut(s) 99
BtgI CCRYGG 1 cut(s) 552
BtsCI GGATG 2 cut(s) 103, 167
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 3 cut(s) 229, 403, 495
BveI ACCTGC 1 cut(s) 82
Cac8I GCNNGC 1 cut(s) 101
CciI TCATGA 1 cut(s) 427
CfoI GCGC 2 cut(s) 396, 620
Cfr13I GGNCC 2 cut(s) 235, 556
CviAII CATG 4 cut(s) 22, 350, 428, 553
CviJI RGCY 9 cut(s) 29, 86, 99, 148, 189, 260, 502, 540, 602
CviKI_1 RGCY 9 cut(s) 29, 86, 99, 148, 189, 260, 502, 540, 602
DdeI CTNAG 4 cut(s) 261, 273, 414, 498
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 2 cut(s) 540, 602
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 2 cut(s) 542, 604
Eco47I GGWCC 2 cut(s) 235, 556
Eco53kI GAGCTC 2 cut(s) 540, 602
Eco81I CCTNAGG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 183
EcoICRI GAGCTC 2 cut(s) 540, 602
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 167
EcoT38I GRGCYC 2 cut(s) 542, 604
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 1 cut(s) 612
FaeI CATG 4 cut(s) 25, 353, 431, 556
FatI CATG 4 cut(s) 21, 349, 427, 552
FauI CCCGC 1 cut(s) 536
Fnu4HI GCNGC 3 cut(s) 423, 490, 503
FokI GGATG 2 cut(s) 90, 174
FriOI GRGCYC 2 cut(s) 542, 604
Fsp4HI GCNGC 3 cut(s) 423, 490, 503
FspBI CTAG 2 cut(s) 176, 318
GlaI GCGC 2 cut(s) 395, 619
GluI GCNGC 3 cut(s) 423, 490, 503
GsuI CTGGAG 1 cut(s) 66
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 1 cut(s) 99
HapII CCGG 1 cut(s) 238
HhaI GCGC 2 cut(s) 396, 620
Hin1II CATG 4 cut(s) 25, 353, 431, 556
Hin6I GCGC 2 cut(s) 394, 618
HinP1I GCGC 2 cut(s) 394, 618
HinfI GANTC 2 cut(s) 201, 458
HpaII CCGG 1 cut(s) 238
HphI GGTGA 2 cut(s) 145, 155
Hpy188I TCNGA 2 cut(s) 300, 510
Hpy188III TCNNGA 3 cut(s) 377, 428, 477
Hpy99I CGWCG 2 cut(s) 95, 98
HpyAV CCTTC 3 cut(s) 97, 226, 425
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4V TGCA 3 cut(s) 165, 285, 549
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 154, 537, 546
HpyF3I CTNAG 4 cut(s) 261, 273, 414, 498
Hsp92II CATG 4 cut(s) 25, 353, 431, 556
HspAI GCGC 2 cut(s) 394, 618
Kzo9I GATC 1 cut(s) 373
LguI GCTCTTC 1 cut(s) 506
LmnI GCTCC 2 cut(s) 82, 607
Lsp1109I GCAGC 2 cut(s) 409, 501
LweI GCATC 5 cut(s) 72, 112, 152, 456, 501
MaeI CTAG 2 cut(s) 176, 318
MaeIII GTNAC 2 cut(s) 115, 320
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 2 cut(s) 136, 523
MfeI CAATTG 1 cut(s) 453
MhlI GDGCHC 2 cut(s) 542, 604
MluCI AATT 3 cut(s) 357, 365, 453
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 2 cut(s) 258, 416
MnlI CCTC 8 cut(s) 59, 90, 159, 192, 234, 282, 409, 587
Mph1103I ATGCAT 1 cut(s) 167
MseI TTAA 1 cut(s) 195
MspI CCGG 1 cut(s) 238
MunI CAATTG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 287
MwoI GCNNNNNNNGC 4 cut(s) 83, 154, 537, 546
NcoI CCATGG 1 cut(s) 552
NdeII GATC 1 cut(s) 373
NlaIII CATG 4 cut(s) 25, 353, 431, 556
NmuCI GTSAC 1 cut(s) 320
NsiI ATGCAT 1 cut(s) 167
PagI TCATGA 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 506
PctI GAATGC 1 cut(s) 287
PfeI GAWTC 1 cut(s) 201
PkrI GCNGC 3 cut(s) 424, 491, 504
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
PshBI ATTAAT 1 cut(s) 195
Psp124BI GAGCTC 2 cut(s) 542, 604
PspPI GGNCC 2 cut(s) 235, 556
SacI GAGCTC 2 cut(s) 542, 604
SapI GCTCTTC 1 cut(s) 506
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 3 cut(s) 423, 490, 503
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 2 cut(s) 235, 556
SchI GAGTC 1 cut(s) 452
SduI GDGCHC 2 cut(s) 542, 604
SfaNI GCATC 5 cut(s) 72, 112, 152, 456, 501
SfcI CTRYAG 1 cut(s) 58
SinI GGWCC 2 cut(s) 235, 556
Sse9I AATT 3 cut(s) 357, 365, 453
SsiI CCGC 4 cut(s) 11, 469, 503, 529
SspMI CTAG 2 cut(s) 176, 318
SstI GAGCTC 2 cut(s) 542, 604
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 1 cut(s) 289
TaqI TCGA 3 cut(s) 39, 66, 90
TasI AATT 3 cut(s) 357, 365, 453
TauI GCSGC 1 cut(s) 505
TfiI GAWTC 1 cut(s) 201
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 3 cut(s) 229, 403, 502
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 2 cut(s) 422, 489
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 2 cut(s) 324, 444
TspGWI ACGGA 2 cut(s) 317, 594
TspRI CASTG 3 cut(s) 229, 403, 502
VpaK11BI GGWCC 2 cut(s) 235, 556
VspI ATTAAT 1 cut(s) 195
XapI RAATTY 1 cut(s) 365
XspI CTAG 2 cut(s) 176, 318
Zsp2I ATGCAT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.