MD02G1262000.v1.1

AMMECR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004168
Physical Location & Seq
Reverse (-)
31625855 .. 31631303
5449 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1262000.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGTGTCGGCGAACAAGGACATGGTGGCTTACTGCTTCGATACTCTCCTCGCTCACTACACCACCGACCAACCTCCTCCCCCTGCTTTCGACGACGGCCAGCATCCATTGTTTGTTACTTGGAAGAAAGTGATGAATGGTGGTGAGCCTCGTTTGCGTGGATGCATAGGAACACTAGAAGCTCGGGGCTTGATTAATGGCTTCAGGGACTATGCCCTAAACAGTGCTTTGAAGGACCGGAGGTTTCCCCCAATACAGGCTAAGGAAATACCTTATTTGGAATGCACAGTTTCCATTCTGACTGATTATGAAACTGCTAGTAACTACCTCGATTGGGAGATTGGAAAACATGGTATAATTATTGAGTTTACTGATCCTGACTATAACACAAGACGCAGCGCCACGTATTTGCCTGAGATTGCTGCCCATGAAGGTTGGACAAAAACCGAAGCAATTGACTCATTGATGCGGAAATCTGGTTACAATGGGAGCATCACTGAGTCGCTTCGGAACCGTATACGGTTGACCCGCTACCAGAGCTCCTTATGCACGATGGACCATAATGACTATGTTTCCTATGTAAAGGAAACCAGAGGCGGAGCTCCGTGTGTAGTTGGTGCTAAGTCTGGCAGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0044424 GO:0044444 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMMECR1

Protein Analysis

213

Amino Acids

23.86

Weight (kDa)

6.52

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMMECR1 PF01871 9 - 181 3.7e-44 AMMECR1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 517
AciI CCGC 4 cut(s) 469, 529, 597, 634
AclWI GGATC 1 cut(s) 368
AcoI YGGCCR 1 cut(s) 97
AcuI CTGAAG 1 cut(s) 187
AfiI CCNNNNNNNGG 2 cut(s) 256, 334
AgsI TTSAA 1 cut(s) 232
AluBI AGCT 3 cut(s) 182, 540, 602
AluI AGCT 3 cut(s) 182, 540, 602
Alw21I GWGCWC 2 cut(s) 542, 604
AlwI GGATC 1 cut(s) 368
AlwNI CAGNNNCTG 1 cut(s) 636
Ama87I CYCGRG 1 cut(s) 183
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 3 cut(s) 396, 422, 630
AseI ATTAAT 1 cut(s) 195
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 2 cut(s) 235, 556
AsuHPI GGTGA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 2 cut(s) 235, 556
BanII GRGCYC 2 cut(s) 542, 604
Bbv12I GWGCWC 2 cut(s) 542, 604
BbvI GCAGC 2 cut(s) 408, 409
BccI CCATC 1 cut(s) 547
BceAI ACGGC 1 cut(s) 112
BfaI CTAG 2 cut(s) 176, 318
BfoI RGCGCY 1 cut(s) 402
BisI GCNGC 4 cut(s) 397, 423, 631, 634
BlsI GCNGC 4 cut(s) 398, 424, 632, 635
Bme18I GGWCC 2 cut(s) 235, 556
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 235, 556
BmiI GGNNCC 1 cut(s) 512
BmsI GCATC 4 cut(s) 112, 152, 456, 501
Bpu10I CCTNAGC 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 405
BsaWI WCCGGW 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 334
BseGI GGATG 2 cut(s) 103, 167
BseLI CCNNNNNNNGG 2 cut(s) 256, 334
BseMII CTCAG 2 cut(s) 405, 489
BseRI GAGGAG 2 cut(s) 38, 66
BseXI GCAGC 2 cut(s) 408, 409
BshFI GGCC 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 542, 604
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 1 cut(s) 238
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 256, 334
BsmFI GGGAC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 1 cut(s) 99
BsoBI CYCGRG 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 542, 604
Bsp143I GATC 1 cut(s) 373
BspACI CCGC 4 cut(s) 469, 529, 597, 634
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 2 cut(s) 406, 490
BspLI GGNNCC 1 cut(s) 512
BspPI GGATC 1 cut(s) 368
BssMI GATC 1 cut(s) 373
BssNAI GTATAC 1 cut(s) 518
Bst1107I GTATAC 1 cut(s) 518
Bst4CI ACNGT 4 cut(s) 224, 289, 515, 522
BstBAI YACGTR 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 4 cut(s) 261, 414, 498, 621
BstF5I GGATG 2 cut(s) 103, 167
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 1 cut(s) 376
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 3 cut(s) 154, 537, 546
BstV1I GCAGC 2 cut(s) 408, 409
BstZ17I GTATAC 1 cut(s) 518
BsuRI GGCC 1 cut(s) 99
BtsCI GGATG 2 cut(s) 103, 167
BtsIMutI CAGTG 2 cut(s) 229, 495
Cac8I GCNNGC 1 cut(s) 101
CaiI CAGNNNCTG 1 cut(s) 636
CfoI GCGC 1 cut(s) 401
Cfr13I GGNCC 2 cut(s) 235, 556
CseI GACGC 1 cut(s) 402
CviAII CATG 3 cut(s) 22, 350, 428
DdeI CTNAG 4 cut(s) 261, 414, 498, 621
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 97
EciI GGCGGA 1 cut(s) 612
Ecl136II GAGCTC 2 cut(s) 540, 602
Eco24I GRGCYC 2 cut(s) 542, 604
Eco47I GGWCC 2 cut(s) 235, 556
Eco53kI GAGCTC 2 cut(s) 540, 602
Eco57I CTGAAG 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 183
EcoICRI GAGCTC 2 cut(s) 540, 602
EcoT22I ATGCAT 1 cut(s) 167
EcoT38I GRGCYC 2 cut(s) 542, 604
FaeI CATG 3 cut(s) 25, 353, 431
FaqI GGGAC 1 cut(s) 221
FatI CATG 3 cut(s) 21, 349, 427
FauI CCCGC 1 cut(s) 536
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 4 cut(s) 397, 423, 631, 634
FokI GGATG 2 cut(s) 90, 174
FriOI GRGCYC 2 cut(s) 542, 604
Fsp4HI GCNGC 4 cut(s) 397, 423, 631, 634
FspBI CTAG 2 cut(s) 176, 318
GlaI GCGC 1 cut(s) 400
GluI GCNGC 4 cut(s) 397, 423, 631, 634
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 1 cut(s) 99
HapII CCGG 1 cut(s) 238
HgaI GACGC 1 cut(s) 402
HhaI GCGC 1 cut(s) 401
Hin1II CATG 3 cut(s) 25, 353, 431
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HincII GTYRAC 1 cut(s) 525
HindII GTYRAC 1 cut(s) 525
HinfI GANTC 2 cut(s) 458, 500
HpaII CCGG 1 cut(s) 238
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 3 cut(s) 369, 518, 525
Hpy188I TCNGA 2 cut(s) 300, 510
Hpy188III TCNNGA 1 cut(s) 377
Hpy8I GTNNAC 3 cut(s) 369, 518, 525
Hpy99I CGWCG 2 cut(s) 95, 98
HpyAV CCTTC 2 cut(s) 226, 425
HpyCH4III ACNGT 4 cut(s) 224, 289, 515, 522
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 3 cut(s) 165, 285, 549
HpyF10VI GCNNNNNNNGC 3 cut(s) 154, 537, 546
HpyF3I CTNAG 4 cut(s) 261, 414, 498, 621
HpySE526I ACGT 1 cut(s) 404
Hsp92II CATG 3 cut(s) 25, 353, 431
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 1 cut(s) 373
LmnI GCTCC 4 cut(s) 489, 545, 599, 607
Lsp1109I GCAGC 2 cut(s) 408, 409
LweI GCATC 4 cut(s) 112, 152, 456, 501
MaeI CTAG 2 cut(s) 176, 318
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 3 cut(s) 115, 320, 479
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 1 cut(s) 136
MfeI CAATTG 1 cut(s) 453
MhlI GDGCHC 2 cut(s) 542, 604
MluCI AATT 2 cut(s) 357, 453
MlyI GAGTC 2 cut(s) 452, 509
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 7 cut(s) 59, 84, 87, 159, 234, 338, 587
Mph1103I ATGCAT 1 cut(s) 167
MseI TTAA 1 cut(s) 195
MspA1I CMGCKG 1 cut(s) 636
MspI CCGG 1 cut(s) 238
MunI CAATTG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 287
MwoI GCNNNNNNNGC 3 cut(s) 154, 537, 546
NdeII GATC 1 cut(s) 373
NlaIII CATG 3 cut(s) 25, 353, 431
NlaIV GGNNCC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 167
PctI GAATGC 1 cut(s) 287
PkrI GCNGC 4 cut(s) 398, 424, 632, 635
PleI GAGTC 2 cut(s) 452, 508
PpsI GAGTC 2 cut(s) 452, 508
Ppu21I YACGTR 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 195
Psp124BI GAGCTC 2 cut(s) 542, 604
PspN4I GGNNCC 1 cut(s) 512
PspPI GGNCC 2 cut(s) 235, 556
PstNI CAGNNNCTG 1 cut(s) 636
SacI GAGCTC 2 cut(s) 542, 604
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 4 cut(s) 397, 423, 631, 634
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 2 cut(s) 235, 556
SchI GAGTC 2 cut(s) 452, 509
SduI GDGCHC 2 cut(s) 542, 604
SetI ASST 9 cut(s) 76, 184, 245, 274, 330, 407, 436, 542, 604
SfaNI GCATC 4 cut(s) 112, 152, 456, 501
SinI GGWCC 2 cut(s) 235, 556
Sse9I AATT 2 cut(s) 357, 453
SsiI CCGC 4 cut(s) 469, 529, 597, 634
SspMI CTAG 2 cut(s) 176, 318
SstI GAGCTC 2 cut(s) 542, 604
TaaI ACNGT 4 cut(s) 224, 289, 515, 522
TaiI ACGT 1 cut(s) 407
TaqI TCGA 3 cut(s) 39, 90, 330
TasI AATT 2 cut(s) 357, 453
TauI GCSGC 1 cut(s) 636
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 2 cut(s) 229, 502
TseI GCWGC 3 cut(s) 396, 422, 630
TspDTI ATGAA 3 cut(s) 149, 324, 444
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 2 cut(s) 229, 502
VpaK11BI GGWCC 2 cut(s) 235, 556
VspI ATTAAT 1 cut(s) 195
XmiI GTMKAC 1 cut(s) 517
XspI CTAG 2 cut(s) 176, 318
Zsp2I ATGCAT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.