Rorug07G0042800

AMMECR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004168
Physical Location & Seq
Forward (+)
3030473 .. 3036094
5622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0042800.1

Sequence Viewer

Length: 1287 bp
ATGTCGGGTTTGAGGTCAACGACAGTTCACCACGCTAAACGACAAACCAGAGCTCAGTCGCTCTCCTCCACTGCTGATCTCGTTTTGCATGTCGGGTTTGAGGTCAACGACGAGACCATGAGCGTGCTGGAGTTGTGGGAGGGCGTCCGAGCAACCCAACCATGTCCACCGTCGTCACCGCCGTCCACCCCAAACCCCGCCCGGTCCCACCCCCTTGCTAGACCCAGGACAAGGCCCCGCTCCCTCATCCTCGCCTATCGCGACCGTTGGTTCTCTCTCCGCCGCACCAATCCAAAATCAGCTGACTGGGACCAAGTCGCTGCCGCCACTGCTTCCTTGGCTCCCTCCTCGCCGCTCAAGACCGCGTTGCAGTGTCGTCATAAGATAGAGAAGCTTCGCAAGCGGTACCATACTGAGAAGCTACGAGCTTCTAGGTACCCGAGTCGGTTTTCCTTGTCGTGGGCTTTGTTTCATCTCATTGATCAAATGGAGTCCGACTCGGATCGGGAATTGGATAATGGTGGTGAGTTTGGAGGTGGGTTTTCGTTAAAGGTTCGTAATTTGGTGAAATTTGATGGAAATGTTGATAGGGATTTTAGTCCTGATGTCGATGAGAATTCTAGCTCAGGAAAGAAAAATGTTGATTACTTGTGGTTTTGTAGCTTTGATAATGTGCTCTTGTTTTGCTGTGATTTGGTTGGAATTCTTGGTCTGAGGAGTCAGAGCTCCGTTGAAGAAGTGAAAGAGGCCTACGACAACGTTTCTTCGACGCGGAATTCAAAAGCGCAAGCTCCGGATTCTCTTGAATTTTATAAAGGTCAGTTGATTCCTGGTGTTGCCTTAAGAATTATTCGATATGCTTATGAGTTGCTGACAAATCCATCATGGAAAAGAAACTATGACATTTTTGGCTTTGAGGAGCAAATAGATGTAATTGAGAAGCTAAAAAAGAAATATGCTGGAGAAAGTTTTTCACGTATACACCTTCCTTTACTAGAGTCTGCTGCTTCTGATTCTGAAGATCATAACCTTCCAGTCATTACCTCTAAGGATTTCCAGACCATGTTTCAGGACAACAAGCCCTGGCTAATTCAGGCCTTCCTTCTTTTGTTGCTTTTCCCCAGGGATGTAAAAGTGCCAAGTGCCTTATTAGAAAAGTTTGCTGTTCAAGCTGGAACTTTCAAGGAAGATGATCAGATTGATTTGCCACCTGGGTTTCGATTCCACCCAACTGATGAAGAGCTTATTTCTCACTGTACATACATGTACATTTACAAGCATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0044424 GO:0044444 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMMECR1

Protein Analysis

428

Amino Acids

48.77

Weight (kDa)

7.19

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 81 - 163 7.3e-10 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 813
Acc65I GGTACC 2 cut(s) 405, 435
AccB1I GGYRCC 2 cut(s) 405, 435
AccBSI CCGCTC 2 cut(s) 240, 355
AccI GTMKAC 1 cut(s) 979
AccII CGCG 3 cut(s) 261, 365, 772
AccIII TCCGGA 1 cut(s) 793
AclI AACGTT 1 cut(s) 759
AclWI GGATC 1 cut(s) 510
AcsI RAATTY 5 cut(s) 569, 616, 702, 775, 806
AcuI CTGAAG 1 cut(s) 1038
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 4 cut(s) 407, 437, 1258, 1268
AfiI CCNNNNNNNGG 2 cut(s) 231, 459
AflII CTTAAG 1 cut(s) 841
AflIII ACRYGT 1 cut(s) 1263
AgsI TTSAA 5 cut(s) 734, 780, 806, 1169, 1183
AjnI CCWGG 5 cut(s) 224, 829, 1082, 1121, 1210
Alw21I GWGCWC 3 cut(s) 55, 678, 728
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 1 cut(s) 510
Ama87I CYCGRG 1 cut(s) 439
Aor13HI TCCGGA 1 cut(s) 793
AoxI GGCC 3 cut(s) 233, 747, 1095
ApeKI GCWGC 2 cut(s) 320, 1004
ApoI RAATTY 5 cut(s) 569, 616, 702, 775, 806
Asp718I GGTACC 2 cut(s) 405, 435
AspLEI GCGC 1 cut(s) 787
AspS9I GGNCC 3 cut(s) 204, 234, 310
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 4 cut(s) 20, 168, 536, 577
AvaI CYCGRG 1 cut(s) 439
AvaII GGWCC 2 cut(s) 204, 310
BaeI ACNNNNGTAYC 2 cut(s) 427, 460
BanI GGYRCC 2 cut(s) 405, 435
BanII GRGCYC 2 cut(s) 55, 728
Bbv12I GWGCWC 3 cut(s) 55, 678, 728
BbvI GCAGC 2 cut(s) 307, 991
BccI CCATC 2 cut(s) 569, 889
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 5 cut(s) 226, 831, 1084, 1123, 1212
BclI TGATCA 2 cut(s) 481, 1192
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 4 cut(s) 219, 432, 621, 995
BfrI CTTAAG 1 cut(s) 841
BisI GCNGC 5 cut(s) 283, 321, 324, 353, 1005
BlsI GCNGC 5 cut(s) 284, 322, 325, 354, 1006
Bme1390I CCNGG 6 cut(s) 202, 226, 831, 1084, 1123, 1212
Bme18I GGWCC 2 cut(s) 204, 310
BmeT110I CYCGRG 1 cut(s) 439
BmgT120I GGNCC 3 cut(s) 204, 234, 310
BmiI GGNNCC 6 cut(s) 206, 236, 311, 342, 407, 437
BmrFI CCNGG 6 cut(s) 202, 226, 831, 1084, 1123, 1212
BmrI ACTGGG 1 cut(s) 316
BmuI ACTGGG 1 cut(s) 316
BplI GAGNNNNNCTC 2 cut(s) 482, 514
BpmI CTGGAG 2 cut(s) 149, 981
Bpu10I CCTNAGC 1 cut(s) 625
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 202
BsaAI YACGTR 1 cut(s) 977
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 6 cut(s) 224, 336, 1082, 1121, 1122, 1211
BsaWI WCCGGW 1 cut(s) 793
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 459
Bse1I ACTGG 2 cut(s) 311, 1034
BseAI TCCGGA 1 cut(s) 793
BseBI CCWGG 5 cut(s) 226, 831, 1084, 1123, 1212
BseDI CCNNGG 6 cut(s) 224, 336, 1082, 1121, 1122, 1211
BseGI GGATG 2 cut(s) 246, 1132
BseLI CCNNNNNNNGG 2 cut(s) 231, 459
BseMII CTCAG 4 cut(s) 68, 405, 639, 704
BseNI ACTGG 2 cut(s) 311, 1034
BseRI GAGGAG 4 cut(s) 55, 337, 730, 932
BseXI GCAGC 2 cut(s) 307, 991
Bsh1236I CGCG 3 cut(s) 261, 365, 772
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 3 cut(s) 235, 749, 1097
BshNI GGYRCC 2 cut(s) 405, 435
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 3 cut(s) 55, 678, 728
BsiHKCI CYCGRG 1 cut(s) 439
BsiSI CCGG 2 cut(s) 202, 794
BslFI GGGAC 2 cut(s) 190, 323
BslI CCNNNNNNNGG 2 cut(s) 231, 459
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 2 cut(s) 190, 323
BsnI GGCC 3 cut(s) 235, 749, 1097
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 439
Bsp1286I GDGCHC 3 cut(s) 55, 678, 728
Bsp13I TCCGGA 1 cut(s) 793
Bsp1407I TGTACA 2 cut(s) 1256, 1266
Bsp143I GATC 5 cut(s) 76, 481, 502, 1021, 1192
Bsp68I TCGCGA 1 cut(s) 261
BspANI GGCC 3 cut(s) 235, 749, 1097
BspCNI CTCAG 4 cut(s) 67, 406, 638, 705
BspEI TCCGGA 1 cut(s) 793
BspFNI CGCG 3 cut(s) 261, 365, 772
BspLI GGNNCC 6 cut(s) 206, 236, 311, 342, 407, 437
BspPI GGATC 1 cut(s) 510
BspQI GCTCTTC 1 cut(s) 1233
BspT107I GGYRCC 2 cut(s) 405, 435
BspTI CTTAAG 1 cut(s) 841
BspTNI GGTCTC 1 cut(s) 107
BsrBI CCGCTC 2 cut(s) 240, 355
BsrGI TGTACA 2 cut(s) 1256, 1266
BsrI ACTGG 2 cut(s) 311, 1034
BssECI CCNNGG 6 cut(s) 224, 336, 1082, 1121, 1122, 1211
BssMI GATC 5 cut(s) 76, 481, 502, 1021, 1192
BssNAI GTATAC 1 cut(s) 980
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 336
Bst1107I GTATAC 1 cut(s) 980
Bst2UI CCWGG 5 cut(s) 226, 831, 1084, 1123, 1212
Bst4CI ACNGT 4 cut(s) 25, 171, 266, 1256
Bst6I CTCTTC 1 cut(s) 1233
BstACI GRCGYC 1 cut(s) 144
BstAFI CTTAAG 1 cut(s) 841
BstAUI TGTACA 2 cut(s) 1256, 1266
BstBAI YACGTR 1 cut(s) 977
BstC8I GCNNGC 3 cut(s) 125, 401, 789
BstDEI CTNAG 5 cut(s) 54, 414, 625, 713, 1047
BstF5I GGATG 2 cut(s) 246, 1132
BstFNI CGCG 3 cut(s) 261, 365, 772
BstHHI GCGC 1 cut(s) 787
BstKTI GATC 5 cut(s) 79, 484, 505, 1024, 1195
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 5 cut(s) 76, 481, 502, 1021, 1192
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 4 cut(s) 329, 338, 400, 1169
BstNI CCWGG 5 cut(s) 226, 831, 1084, 1123, 1212
BstNSI RCATGY 2 cut(s) 92, 1267
BstSCI CCNGG 6 cut(s) 200, 224, 829, 1082, 1121, 1210
BstUI CGCG 3 cut(s) 261, 365, 772
BstV1I GCAGC 2 cut(s) 307, 991
BstZ17I GTATAC 1 cut(s) 980
BsuRI GGCC 3 cut(s) 235, 749, 1097
BtsCI GGATG 2 cut(s) 246, 1132
BtsI GCAGTG 3 cut(s) 69, 327, 377
BtsIMutI CAGTG 4 cut(s) 69, 327, 377, 1252
BtuMI TCGCGA 1 cut(s) 261
Cac8I GCNNGC 3 cut(s) 125, 401, 789
CfoI GCGC 1 cut(s) 787
Cfr13I GGNCC 3 cut(s) 204, 234, 310
CseI GACGC 2 cut(s) 133, 778
Csp6I GTAC 4 cut(s) 406, 436, 1257, 1267
CviAII CATG 6 cut(s) 89, 118, 162, 885, 1063, 1264
CviQI GTAC 4 cut(s) 406, 436, 1257, 1267
DdeI CTNAG 5 cut(s) 54, 414, 625, 713, 1047
DpnI GATC 5 cut(s) 78, 483, 504, 1023, 1194
DpnII GATC 5 cut(s) 76, 481, 502, 1021, 1192
Eam1104I CTCTTC 1 cut(s) 1233
EarI CTCTTC 1 cut(s) 1233
EciI GGCGGA 1 cut(s) 269
Ecl136II GAGCTC 2 cut(s) 53, 726
Eco130I CCWWGG 1 cut(s) 336
Eco147I AGGCCT 2 cut(s) 749, 1097
Eco24I GRGCYC 2 cut(s) 55, 728
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 2 cut(s) 204, 310
Eco53kI GAGCTC 2 cut(s) 53, 726
Eco57I CTGAAG 1 cut(s) 1038
Eco88I CYCGRG 1 cut(s) 439
EcoICRI GAGCTC 2 cut(s) 53, 726
EcoO109I RGGNCCY 1 cut(s) 234
EcoRI GAATTC 3 cut(s) 616, 702, 775
EcoRII CCWGG 5 cut(s) 224, 829, 1082, 1121, 1210
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 2 cut(s) 55, 728
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 6 cut(s) 92, 121, 165, 888, 1066, 1267
FaqI GGGAC 2 cut(s) 190, 323
FatI CATG 6 cut(s) 88, 117, 161, 884, 1062, 1263
FauI CCCGC 2 cut(s) 205, 245
FbaI TGATCA 2 cut(s) 481, 1192
FblI GTMKAC 1 cut(s) 979
Fnu4HI GCNGC 5 cut(s) 283, 321, 324, 353, 1005
FokI GGATG 2 cut(s) 233, 1139
FriOI GRGCYC 2 cut(s) 55, 728
Fsp4HI GCNGC 5 cut(s) 283, 321, 324, 353, 1005
FspBI CTAG 4 cut(s) 219, 432, 621, 995
GlaI GCGC 1 cut(s) 786
GluI GCNGC 5 cut(s) 283, 321, 324, 353, 1005
GsuI CTGGAG 2 cut(s) 149, 981
HaeIII GGCC 3 cut(s) 235, 749, 1097
HapII CCGG 2 cut(s) 202, 794
HgaI GACGC 2 cut(s) 133, 778
HhaI GCGC 1 cut(s) 787
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 6 cut(s) 92, 121, 165, 888, 1066, 1267
Hin6I GCGC 1 cut(s) 785
HinP1I GCGC 1 cut(s) 785
HincII GTYRAC 2 cut(s) 18, 106
HindII GTYRAC 2 cut(s) 18, 106
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 9 cut(s) 442, 491, 497, 718, 797, 826, 998, 1013, 1221
HpaII CCGG 2 cut(s) 202, 794
HphI GGTGA 4 cut(s) 20, 168, 536, 577
Hpy166II GTNNAC 6 cut(s) 18, 28, 106, 167, 186, 980
Hpy188I TCNGA 8 cut(s) 149, 496, 502, 714, 723, 1012, 1018, 1197
Hpy188III TCNNGA 9 cut(s) 260, 358, 506, 602, 627, 794, 803, 1057, 1070
Hpy8I GTNNAC 6 cut(s) 18, 28, 106, 167, 186, 980
Hpy99I CGWCG 3 cut(s) 113, 175, 772
HpyAV CCTTC 4 cut(s) 995, 1040, 1108, 1112
HpyCH4III ACNGT 4 cut(s) 25, 171, 266, 1256
HpyCH4IV ACGT 2 cut(s) 759, 976
HpyCH4V TGCA 2 cut(s) 88, 370
HpyF10VI GCNNNNNNNGC 4 cut(s) 329, 338, 400, 1169
HpyF3I CTNAG 5 cut(s) 54, 414, 625, 713, 1047
HpySE526I ACGT 2 cut(s) 759, 976
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 6 cut(s) 92, 121, 165, 888, 1066, 1267
HspAI GCGC 1 cut(s) 785
Kpn2I TCCGGA 1 cut(s) 793
KpnI GGTACC 2 cut(s) 409, 439
Ksp22I TGATCA 2 cut(s) 481, 1192
Kzo9I GATC 5 cut(s) 76, 481, 502, 1021, 1192
LguI GCTCTTC 1 cut(s) 1233
LmnI GCTCC 5 cut(s) 245, 346, 731, 796, 919
Lsp1109I GCAGC 2 cut(s) 307, 991
MaeI CTAG 4 cut(s) 219, 432, 621, 995
MaeII ACGT 2 cut(s) 759, 976
MaeIII GTNAC 1 cut(s) 174
MalI GATC 5 cut(s) 78, 483, 504, 1023, 1194
MbiI CCGCTC 2 cut(s) 240, 355
MboI GATC 5 cut(s) 76, 481, 502, 1021, 1192
MboII GAAGA 5 cut(s) 746, 756, 1031, 1199, 1250
MhlI GDGCHC 3 cut(s) 55, 678, 728
MlyI GAGTC 5 cut(s) 451, 491, 500, 727, 1007
MmeI TCCRAC 2 cut(s) 519, 679
MroI TCCGGA 1 cut(s) 793
MseI TTAA 2 cut(s) 548, 842
MslI CAYNNNNRTG 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 841
MspI CCGG 2 cut(s) 202, 794
MspR9I CCNGG 6 cut(s) 202, 226, 831, 1084, 1123, 1212
MvaI CCWGG 5 cut(s) 226, 831, 1084, 1123, 1212
MvnI CGCG 3 cut(s) 261, 365, 772
MwoI GCNNNNNNNGC 4 cut(s) 329, 338, 400, 1169
NciI CCSGG 1 cut(s) 202
NdeII GATC 5 cut(s) 76, 481, 502, 1021, 1192
NlaIII CATG 6 cut(s) 92, 121, 165, 888, 1066, 1267
NlaIV GGNNCC 6 cut(s) 206, 236, 311, 342, 407, 437
NmuCI GTSAC 1 cut(s) 174
NruI TCGCGA 1 cut(s) 261
NspI RCATGY 2 cut(s) 92, 1267
PasI CCCWGGG 1 cut(s) 1122
PceI AGGCCT 2 cut(s) 749, 1097
PciI ACATGT 1 cut(s) 1263
PciSI GCTCTTC 1 cut(s) 1233
PcsI WCGNNNNNNNCGW 2 cut(s) 179, 258
PfeI GAWTC 4 cut(s) 797, 826, 1013, 1221
PflFI GACNNNGTC 1 cut(s) 314
PkrI GCNGC 5 cut(s) 284, 322, 325, 354, 1006
PleI GAGTC 5 cut(s) 450, 491, 499, 726, 1006
PpsI GAGTC 5 cut(s) 450, 491, 499, 726, 1006
Ppu21I YACGTR 1 cut(s) 977
PscI ACATGT 1 cut(s) 1263
PsiI TTATAA 1 cut(s) 813
Psp124BI GAGCTC 2 cut(s) 55, 728
Psp1406I AACGTT 1 cut(s) 759
Psp6I CCWGG 5 cut(s) 224, 829, 1082, 1121, 1210
PspGI CCWGG 5 cut(s) 224, 829, 1082, 1121, 1210
PspN4I GGNNCC 6 cut(s) 206, 236, 311, 342, 407, 437
PspPI GGNCC 3 cut(s) 204, 234, 310
PsyI GACNNNGTC 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 302
RruI TCGCGA 1 cut(s) 261
RsaI GTAC 4 cut(s) 407, 437, 1258, 1268
RsaNI GTAC 4 cut(s) 406, 436, 1257, 1267
RseI CAYNNNNRTG 1 cut(s) 122
SacI GAGCTC 2 cut(s) 55, 728
SapI GCTCTTC 1 cut(s) 1233
SaqAI TTAA 2 cut(s) 548, 842
SatI GCNGC 5 cut(s) 283, 321, 324, 353, 1005
Sau3AI GATC 5 cut(s) 76, 481, 502, 1021, 1192
Sau96I GGNCC 3 cut(s) 204, 234, 310
SchI GAGTC 5 cut(s) 451, 491, 500, 727, 1007
ScrFI CCNGG 6 cut(s) 202, 226, 831, 1084, 1123, 1212
SduI GDGCHC 3 cut(s) 55, 678, 728
SinI GGWCC 2 cut(s) 204, 310
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 356, 841
SmoI CTYRAG 2 cut(s) 356, 841
SseBI AGGCCT 2 cut(s) 749, 1097
SspMI CTAG 4 cut(s) 219, 432, 621, 995
SstI GAGCTC 2 cut(s) 55, 728
StuI AGGCCT 2 cut(s) 749, 1097
StyD4I CCNGG 6 cut(s) 200, 224, 829, 1082, 1121, 1210
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 4 cut(s) 25, 171, 266, 1256
TaiI ACGT 2 cut(s) 762, 979
TaqI TCGA 4 cut(s) 609, 767, 853, 1219
TatI WGTACW 2 cut(s) 1256, 1266
TauI GCSGC 3 cut(s) 285, 326, 355
TfiI GAWTC 4 cut(s) 797, 826, 1013, 1221
Tru1I TTAA 2 cut(s) 548, 842
Tru9I TTAA 2 cut(s) 548, 842
TscAI CASTG 4 cut(s) 76, 334, 377, 1259
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 2 cut(s) 320, 1004
Tsp45I GTSAC 1 cut(s) 174
TspDTI ATGAA 2 cut(s) 461, 1251
TspGWI ACGGA 1 cut(s) 718
TspRI CASTG 4 cut(s) 76, 334, 377, 1259
Tth111I GACNNNGTC 1 cut(s) 314
Vha464I CTTAAG 1 cut(s) 841
VpaK11BI GGWCC 2 cut(s) 204, 310
XapI RAATTY 5 cut(s) 569, 616, 702, 775, 806
XceI RCATGY 2 cut(s) 92, 1267
XcmI CCANNNNNNNNNTGG 2 cut(s) 124, 334
XmiI GTMKAC 1 cut(s) 979
XspI CTAG 4 cut(s) 219, 432, 621, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.