Rw4G021910

AMMECR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004168
Physical Location & Seq
Reverse (-)
46691240 .. 46693964
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G021910.1

Sequence Viewer

Length: 612 bp
ATGCCACTGGCAGCTCTCTCTACCTCCCGTTATCCAATCCCGTCTCGGCTCTCTCTCTCTGCCTCGCCTCCGTCCGCACCTTCAAAGAGCACACCTACCGCGTCTACTCCGCCCTCTTCGCCTCCGTCTCCAGCGACTCTTGTCTGCTGGTTTGCTGAAGAATTAGAATACGGGTGTAGCTCTCTCCTCCCAAATCCAGGTCTTGCTATGAGGGACCGCAGGTTTCCCCCAATACAAGCTAAGGAATTACCATATTTGGAATGTACAGTTTCCATTTTGACTGATTATGAAACTGCTGATGACTACCTTGATTGGGAGGTTGGGAAGCATGGTATAATCATTGAGTTTACTGATCCGGGCTATGGTACAAGGCGCAATGCAACATACTTGCCTGAGGTTGCTGCGCATGAAGGTTGGACTCAAATAGAAGCAATTGACTCATTGATGCGGAAAGCAGGGTATACTGGGACCATCACCGAGTCACTTCGGAAGCGTATACGATTGACCCGGTACCAGAGCTCATTATATACAATGCACCACAGCGACTACGTTTCTTATGTGAAGGCAAGCAGAGGCGCAGCTACATCTGGCTCCAAGTCCAGCAATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0044424 GO:0044444 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMMECR1

Protein Analysis

203

Amino Acids

22.3

Weight (kDa)

6.9

Isoelectric Point (pI)

58.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMMECR1 PF01871 69 - 174 1.2e-28 AMMECR1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 405
Acc36I ACCTGC 1 cut(s) 210
Acc65I GGTACC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 510
AccI GTMKAC 3 cut(s) 104, 461, 496
AccII CGCG 1 cut(s) 101
AciI CCGC 5 cut(s) 75, 99, 110, 217, 448
AclWI GGATC 1 cut(s) 347
AcuI CTGAAG 1 cut(s) 177
AfaI GTAC 3 cut(s) 265, 367, 512
AfiI CCNNNNNNNGG 3 cut(s) 197, 313, 362
AgsI TTSAA 1 cut(s) 84
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 5 cut(s) 14, 180, 239, 519, 581
AluI AGCT 5 cut(s) 14, 180, 239, 519, 581
Alw21I GWGCWC 2 cut(s) 92, 521
Alw26I GTCTC 2 cut(s) 48, 132
AlwI GGATC 1 cut(s) 347
ApeKI GCWGC 3 cut(s) 11, 401, 578
Asp718I GGTACC 1 cut(s) 510
AspLEI GCGC 3 cut(s) 375, 406, 578
AspS9I GGNCC 2 cut(s) 214, 468
AsuC2I CCSGG 2 cut(s) 357, 508
AsuHPI GGTGA 1 cut(s) 466
AvaII GGWCC 2 cut(s) 214, 468
AxyI CCTNAGG 1 cut(s) 393
BanI GGYRCC 1 cut(s) 510
BanII GRGCYC 1 cut(s) 521
Bbv12I GWGCWC 2 cut(s) 92, 521
BbvI GCAGC 3 cut(s) 23, 388, 590
BccI CCATC 1 cut(s) 479
BciT130I CCWGG 1 cut(s) 198
BcnI CCSGG 2 cut(s) 357, 508
BcoDI GTCTC 2 cut(s) 48, 132
BfuAI ACCTGC 1 cut(s) 210
BisI GCNGC 3 cut(s) 12, 402, 579
BlsI GCNGC 3 cut(s) 13, 403, 580
Bme1390I CCNGG 3 cut(s) 198, 357, 508
Bme18I GGWCC 2 cut(s) 214, 468
BmgT120I GGNCC 2 cut(s) 214, 468
BmiI GGNNCC 4 cut(s) 215, 469, 512, 592
BmrFI CCNGG 3 cut(s) 198, 357, 508
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 1 cut(s) 435
BmuI ACTGGG 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 140
BpmI CTGGAG 1 cut(s) 114
Bpu10I CCTNAGC 1 cut(s) 240
BpuMI CCSGG 2 cut(s) 357, 508
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 313, 362
Bse1I ACTGG 2 cut(s) 12, 469
Bse21I CCTNAGG 1 cut(s) 393
Bse3DI GCAATG 1 cut(s) 382
BseBI CCWGG 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 197, 313, 362
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 12, 469
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 3 cut(s) 23, 388, 590
Bsh1236I CGCG 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 510
BsiHKAI GWGCWC 2 cut(s) 92, 521
BsiSI CCGG 2 cut(s) 356, 508
BslFI GGGAC 2 cut(s) 227, 481
BslI CCNNNNNNNGG 3 cut(s) 197, 313, 362
BsmAI GTCTC 2 cut(s) 48, 132
BsmBI CGTCTC 2 cut(s) 48, 132
BsmFI GGGAC 2 cut(s) 227, 481
Bsp1286I GDGCHC 2 cut(s) 92, 521
Bsp1407I TGTACA 1 cut(s) 263
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 5 cut(s) 75, 99, 110, 217, 448
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 1 cut(s) 101
BspLI GGNNCC 4 cut(s) 215, 469, 512, 592
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 1 cut(s) 347
BspT107I GGYRCC 1 cut(s) 510
BsrDI GCAATG 1 cut(s) 382
BsrGI TGTACA 1 cut(s) 263
BsrI ACTGG 2 cut(s) 12, 469
BssMI GATC 1 cut(s) 352
BssNAI GTATAC 2 cut(s) 462, 497
Bst1107I GTATAC 2 cut(s) 462, 497
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 121
BstAUI TGTACA 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 568
BstDEI CTNAG 2 cut(s) 240, 393
BstFNI CGCG 1 cut(s) 101
BstHHI GCGC 3 cut(s) 375, 406, 578
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 2 cut(s) 48, 132
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 1 cut(s) 118
BstNI CCWGG 1 cut(s) 198
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 3 cut(s) 196, 355, 506
BstUI CGCG 1 cut(s) 101
BstV1I GCAGC 3 cut(s) 23, 388, 590
BstZ17I GTATAC 2 cut(s) 462, 497
Bsu36I CCTNAGG 1 cut(s) 393
BtsIMutI CAGTG 1 cut(s) 5
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 568
CfoI GCGC 3 cut(s) 375, 406, 578
Cfr13I GGNCC 2 cut(s) 214, 468
CseI GACGC 1 cut(s) 90
Csp6I GTAC 3 cut(s) 264, 366, 511
CviAII CATG 2 cut(s) 329, 407
CviJI RGCY 8 cut(s) 14, 49, 180, 239, 360, 519, 581, 591
CviKI_1 RGCY 8 cut(s) 14, 49, 180, 239, 360, 519, 581, 591
CviQI GTAC 3 cut(s) 264, 366, 511
DdeI CTNAG 2 cut(s) 240, 393
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 121
EciI GGCGGA 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 521
Eco47I GGWCC 2 cut(s) 214, 468
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 177
Eco81I CCTNAGG 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 519
EcoRII CCWGG 1 cut(s) 196
EcoT38I GRGCYC 1 cut(s) 521
Esp3I CGTCTC 2 cut(s) 48, 132
FaeI CATG 2 cut(s) 332, 410
FaqI GGGAC 2 cut(s) 227, 481
FatI CATG 2 cut(s) 328, 406
FblI GTMKAC 3 cut(s) 104, 461, 496
Fnu4HI GCNGC 3 cut(s) 12, 402, 579
FriOI GRGCYC 1 cut(s) 521
Fsp4HI GCNGC 3 cut(s) 12, 402, 579
FspI TGCGCA 1 cut(s) 405
GlaI GCGC 3 cut(s) 374, 405, 577
GluI GCNGC 3 cut(s) 12, 402, 579
GsuI CTGGAG 1 cut(s) 114
HapII CCGG 2 cut(s) 356, 508
HgaI GACGC 1 cut(s) 90
HhaI GCGC 3 cut(s) 375, 406, 578
Hin1II CATG 2 cut(s) 332, 410
Hin6I GCGC 3 cut(s) 373, 404, 576
HinP1I GCGC 3 cut(s) 373, 404, 576
HinfI GANTC 4 cut(s) 136, 418, 437, 479
HpaII CCGG 2 cut(s) 356, 508
HphI GGTGA 1 cut(s) 466
Hpy166II GTNNAC 4 cut(s) 105, 348, 462, 497
Hpy188I TCNGA 1 cut(s) 489
Hpy8I GTNNAC 4 cut(s) 105, 348, 462, 497
HpyAV CCTTC 3 cut(s) 90, 404, 556
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 2 cut(s) 380, 535
HpyF10VI GCNNNNNNNGC 1 cut(s) 118
HpyF3I CTNAG 2 cut(s) 240, 393
HpySE526I ACGT 1 cut(s) 549
Hsp92II CATG 2 cut(s) 332, 410
HspAI GCGC 3 cut(s) 373, 404, 576
KpnI GGTACC 1 cut(s) 514
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 596
Lsp1109I GCAGC 3 cut(s) 23, 388, 590
LweI GCATC 1 cut(s) 435
MaeII ACGT 1 cut(s) 549
MaeIII GTNAC 1 cut(s) 480
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 2 cut(s) 108, 170
MfeI CAATTG 1 cut(s) 432
MhlI GDGCHC 2 cut(s) 92, 521
MluCI AATT 3 cut(s) 161, 245, 432
MlyI GAGTC 4 cut(s) 130, 412, 431, 488
MmeI TCCRAC 1 cut(s) 395
MspI CCGG 2 cut(s) 356, 508
MspR9I CCNGG 3 cut(s) 198, 357, 508
MunI CAATTG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 198
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 1 cut(s) 118
NciI CCSGG 2 cut(s) 357, 508
NdeII GATC 1 cut(s) 352
NlaIII CATG 2 cut(s) 332, 410
NlaIV GGNNCC 4 cut(s) 215, 469, 512, 592
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 1 cut(s) 480
NsbI TGCGCA 1 cut(s) 405
PkrI GCNGC 3 cut(s) 13, 403, 580
PleI GAGTC 4 cut(s) 130, 412, 431, 487
PpsI GAGTC 4 cut(s) 130, 412, 431, 487
PshAI GACNNNNGTC 1 cut(s) 140
Psp124BI GAGCTC 1 cut(s) 521
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspN4I GGNNCC 4 cut(s) 215, 469, 512, 592
PspPI GGNCC 2 cut(s) 214, 468
RsaI GTAC 3 cut(s) 265, 367, 512
RsaNI GTAC 3 cut(s) 264, 366, 511
SacI GAGCTC 1 cut(s) 521
SatI GCNGC 3 cut(s) 12, 402, 579
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 2 cut(s) 214, 468
SchI GAGTC 4 cut(s) 130, 412, 431, 488
ScrFI CCNGG 3 cut(s) 198, 357, 508
SduI GDGCHC 2 cut(s) 92, 521
SfaNI GCATC 1 cut(s) 435
SinI GGWCC 2 cut(s) 214, 468
Sse9I AATT 3 cut(s) 161, 245, 432
SsiI CCGC 5 cut(s) 75, 99, 110, 217, 448
SstI GAGCTC 1 cut(s) 521
StyD4I CCNGG 3 cut(s) 196, 355, 506
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 1 cut(s) 552
TasI AATT 3 cut(s) 161, 245, 432
TatI WGTACW 1 cut(s) 263
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 1 cut(s) 480
TseI GCWGC 3 cut(s) 11, 401, 578
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 2 cut(s) 303, 423
TspGWI ACGGA 2 cut(s) 60, 114
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 2 cut(s) 214, 468
XmiI GTMKAC 3 cut(s) 104, 461, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.