Rorug07G0042700

AMMECR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004168
Physical Location & Seq
Forward (+)
3017342 .. 3020996
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0042700.1

Sequence Viewer

Length: 957 bp
ATGGGGAAGTCCATAACGTTAACTAACAGCACTGCTACGACTTCGGAGATAAAAAGAGGGCCATGGACTCCTCAAGAGGACAAGAAGCTCTTTGCTTTCATTCAACAACATGGCCATGGAAGCTGGCGGTCCTTGCCCAAGAAAGCTGGTTTGCAAAGATGTGGGAAGAGCTGCAGGCTAAGATGGAGAAACTACCTAAACCCTGCTATTAAGAGAGGAAATTTCAGTTTTCAGGATGACCAGACCATCATTCAGCTTCATGCACTTCTCGGCAACAGGTGGTCAGCCATAGCTGCGAACTTACCAAGGAGAACAGACAATGAGATAAAGAACTACTGGAACACACATCTAAAGAAGAGGTTAGCCAAGACAGGGTTTGATCCTGTAACCCACAAACCCAAAACCGCCATCCTTGCCTCTGCTAATGGCGACCCAAAGAACCTGTCAAACCTCAGCCACATAGCTCAGTGGGAAAGTGCTAGGCTTCAAGCAGAAGCTAGATTTGTGAGACAGTCAAAACTACTCAAACCAGCACCACCAGCACCACAGTGCCTTGACTTCAATTTAAGAACATGGGAAACCCTAATGATGTCAAAGTCATTAGCTGCAGGCGGTGGTGGTGGCGACGAAGCCGGTGGTGTTGGTCGTGCCACTAAGGTTGTTCAAGAGATTGGTTCATTCGGTAATTTTGACGGTGTACTTGGTAATAATGGGAGACAGTTTGAAGTTCCAAAATTAACCATACCAGCAGCTCCGGATTTCAATGAAGTTTCAGAGGAGAGTGGTACTGATGAATACGGTGACCTATTTGAGGCACTACTACTCCAGCAGAGCTACAATAATACTGGGTTTGATGTCGTGGATGCTTGGACTTTGGAACAATGTAATGATATCAGTGCTGACTATCAAAATGTGATCATGAGCGGGGGGTTGAAAAACCTATTTATGTTCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0044424 GO:0044444 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMMECR1

Protein Analysis

318

Amino Acids

35.27

Weight (kDa)

9.2

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 19 - 66 6.4e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 22 - 81 3.4e-11 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 72 - 117 2.2e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 76 - 127 6.3e-09 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 924
AccIII TCCGGA 1 cut(s) 754
AciI CCGC 4 cut(s) 127, 405, 612, 924
AclI AACGTT 1 cut(s) 17
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 1 cut(s) 220
AfaI GTAC 2 cut(s) 699, 787
AfiI CCNNNNNNNGG 2 cut(s) 372, 811
AgsI TTSAA 7 cut(s) 104, 488, 562, 665, 725, 763, 934
AleI CACNNNNGTG 1 cut(s) 547
Alw26I GTCTC 2 cut(s) 502, 709
AlwI GGATC 1 cut(s) 374
Aor13HI TCCGGA 1 cut(s) 754
AoxI GGCC 2 cut(s) 59, 112
ApeKI GCWGC 4 cut(s) 171, 293, 605, 749
ApoI RAATTY 1 cut(s) 220
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AspS9I GGNCC 2 cut(s) 59, 129
AsuHPI GGTGA 1 cut(s) 812
AvaII GGWCC 1 cut(s) 129
BalI TGGCCA 1 cut(s) 114
BbvCI CCTCAGC 1 cut(s) 452
BbvI GCAGC 4 cut(s) 158, 280, 592, 761
BccI CCATC 3 cut(s) 177, 254, 416
BclI TGATCA 1 cut(s) 915
BcoDI GTCTC 2 cut(s) 502, 709
BfaI CTAG 2 cut(s) 480, 498
BfmI CTRYAG 2 cut(s) 172, 606
BisI GCNGC 4 cut(s) 172, 294, 606, 750
BlsI GCNGC 4 cut(s) 173, 295, 607, 751
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 59, 129
BmrI ACTGGG 1 cut(s) 855
BmsI GCATC 1 cut(s) 853
BmuI ACTGGG 1 cut(s) 855
BpmI CTGGAG 1 cut(s) 809
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 57
BsaJI CCNNGG 3 cut(s) 62, 115, 305
BsaWI WCCGGW 1 cut(s) 754
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 2 cut(s) 372, 811
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 3 cut(s) 341, 850, 952
BseAI TCCGGA 1 cut(s) 754
BseDI CCNNGG 3 cut(s) 62, 115, 305
BseGI GGATG 3 cut(s) 241, 408, 868
BseLI CCNNNNNNNGG 2 cut(s) 372, 811
BseMII CTCAG 2 cut(s) 466, 479
BseNI ACTGG 3 cut(s) 341, 850, 952
BseRI GAGGAG 2 cut(s) 60, 791
BseXI GCAGC 4 cut(s) 158, 280, 592, 761
BshFI GGCC 2 cut(s) 61, 114
BsiSI CCGG 2 cut(s) 633, 755
BslI CCNNNNNNNGG 2 cut(s) 372, 811
BsmAI GTCTC 2 cut(s) 502, 709
BsnI GGCC 2 cut(s) 61, 114
Bsp13I TCCGGA 1 cut(s) 754
Bsp143I GATC 2 cut(s) 379, 915
Bsp19I CCATGG 2 cut(s) 62, 115
BspACI CCGC 4 cut(s) 127, 405, 612, 924
BspANI GGCC 2 cut(s) 61, 114
BspCNI CTCAG 2 cut(s) 465, 478
BspEI TCCGGA 1 cut(s) 754
BspHI TCATGA 1 cut(s) 918
BspMAI CTGCAG 2 cut(s) 176, 610
BspPI GGATC 1 cut(s) 374
BspQI GCTCTTC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 924
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 3 cut(s) 341, 850, 952
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 3 cut(s) 62, 115, 305
BssMI GATC 2 cut(s) 379, 915
BssT1I CCWWGG 3 cut(s) 62, 115, 305
Bst4CI ACNGT 5 cut(s) 513, 549, 695, 720, 800
Bst6I CTCTTC 2 cut(s) 161, 350
BstC8I GCNNGC 3 cut(s) 125, 176, 610
BstDEI CTNAG 4 cut(s) 179, 452, 465, 654
BstDSI CCRYGG 2 cut(s) 62, 115
BstEII GGTNACC 1 cut(s) 800
BstENI CCTNNNNNAGG 1 cut(s) 809
BstF5I GGATG 3 cut(s) 241, 408, 868
BstKTI GATC 2 cut(s) 382, 918
BstMAI GTCTC 2 cut(s) 502, 709
BstMBI GATC 2 cut(s) 379, 915
BstMWI GCNNNNNNNGC 5 cut(s) 120, 133, 293, 413, 539
BstPI GGTNACC 1 cut(s) 800
BstSFI CTRYAG 2 cut(s) 172, 606
BstV1I GCAGC 4 cut(s) 158, 280, 592, 761
BsuRI GGCC 2 cut(s) 61, 114
BtgI CCRYGG 2 cut(s) 62, 115
BtsCI GGATG 3 cut(s) 241, 408, 868
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 4 cut(s) 30, 473, 554, 901
Cac8I GCNNGC 3 cut(s) 125, 176, 610
CciI TCATGA 1 cut(s) 918
Cfr10I RCCGGY 1 cut(s) 632
Cfr13I GGNCC 2 cut(s) 59, 129
Csp6I GTAC 2 cut(s) 698, 786
CspCI CAANNNNNGTGG 2 cut(s) 640, 675
CviAII CATG 6 cut(s) 63, 110, 116, 260, 573, 919
CviQI GTAC 2 cut(s) 698, 786
DdeI CTNAG 4 cut(s) 179, 452, 465, 654
DpnI GATC 2 cut(s) 381, 917
DpnII GATC 2 cut(s) 379, 915
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 2 cut(s) 161, 350
EarI CTCTTC 2 cut(s) 161, 350
Eco130I CCWWGG 3 cut(s) 62, 115, 305
Eco32I GATATC 1 cut(s) 892
Eco47I GGWCC 1 cut(s) 129
Eco91I GGTNACC 1 cut(s) 800
EcoNI CCTNNNNNAGG 1 cut(s) 809
EcoO65I GGTNACC 1 cut(s) 800
EcoRV GATATC 1 cut(s) 892
EcoT14I CCWWGG 3 cut(s) 62, 115, 305
ErhI CCWWGG 3 cut(s) 62, 115, 305
FaeI CATG 6 cut(s) 66, 113, 119, 263, 576, 922
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FatI CATG 6 cut(s) 62, 109, 115, 259, 572, 918
FauI CCCGC 1 cut(s) 917
FbaI TGATCA 1 cut(s) 915
Fnu4HI GCNGC 4 cut(s) 172, 294, 606, 750
FokI GGATG 3 cut(s) 248, 395, 875
Fsp4HI GCNGC 4 cut(s) 172, 294, 606, 750
FspBI CTAG 2 cut(s) 480, 498
GluI GCNGC 4 cut(s) 172, 294, 606, 750
GsuI CTGGAG 1 cut(s) 809
HaeIII GGCC 2 cut(s) 61, 114
HapII CCGG 2 cut(s) 633, 755
Hin1II CATG 6 cut(s) 66, 113, 119, 263, 576, 922
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 1 cut(s) 67
HpaI GTTAAC 1 cut(s) 21
HpaII CCGG 2 cut(s) 633, 755
HphI GGTGA 1 cut(s) 812
Hpy166II GTNNAC 2 cut(s) 21, 698
Hpy188I TCNGA 2 cut(s) 46, 775
Hpy188III TCNNGA 5 cut(s) 74, 233, 665, 755, 919
Hpy8I GTNNAC 2 cut(s) 21, 698
Hpy99I CGWCG 1 cut(s) 629
HpyCH4III ACNGT 5 cut(s) 513, 549, 695, 720, 800
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 4 cut(s) 154, 174, 263, 608
HpyF10VI GCNNNNNNNGC 5 cut(s) 120, 133, 293, 413, 539
HpyF3I CTNAG 4 cut(s) 179, 452, 465, 654
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 6 cut(s) 66, 113, 119, 263, 576, 922
Kpn2I TCCGGA 1 cut(s) 754
Ksp22I TGATCA 1 cut(s) 915
KspAI GTTAAC 1 cut(s) 21
Kzo9I GATC 2 cut(s) 379, 915
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 1 cut(s) 757
Lsp1109I GCAGC 4 cut(s) 158, 280, 592, 761
LweI GCATC 1 cut(s) 853
MaeI CTAG 2 cut(s) 480, 498
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 2 cut(s) 385, 800
MalI GATC 2 cut(s) 381, 917
MbiI CCGCTC 1 cut(s) 924
MboI GATC 2 cut(s) 379, 915
MboII GAAGA 2 cut(s) 178, 367
MlsI TGGCCA 1 cut(s) 114
MluCI AATT 4 cut(s) 220, 562, 685, 734
MluNI TGGCCA 1 cut(s) 114
MlyI GAGTC 1 cut(s) 61
MnlI CCTC 9 cut(s) 50, 70, 81, 209, 351, 427, 461, 769, 805
Mox20I TGGCCA 1 cut(s) 114
MroI TCCGGA 1 cut(s) 754
MscI TGGCCA 1 cut(s) 114
MseI TTAA 4 cut(s) 20, 210, 566, 737
MslI CAYNNNNRTG 2 cut(s) 114, 547
Msp20I TGGCCA 1 cut(s) 114
MspI CCGG 2 cut(s) 633, 755
MwoI GCNNNNNNNGC 5 cut(s) 120, 133, 293, 413, 539
NcoI CCATGG 2 cut(s) 62, 115
NdeII GATC 2 cut(s) 379, 915
NlaIII CATG 6 cut(s) 66, 113, 119, 263, 576, 922
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 800
OliI CACNNNNGTG 1 cut(s) 547
PagI TCATGA 1 cut(s) 918
PciSI GCTCTTC 1 cut(s) 161
PkrI GCNGC 4 cut(s) 173, 295, 607, 751
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Psp1406I AACGTT 1 cut(s) 17
PspEI GGTNACC 1 cut(s) 800
PspPI GGNCC 2 cut(s) 59, 129
PstI CTGCAG 2 cut(s) 176, 610
RsaI GTAC 2 cut(s) 699, 787
RsaNI GTAC 2 cut(s) 698, 786
RseI CAYNNNNRTG 2 cut(s) 114, 547
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 4 cut(s) 20, 210, 566, 737
SatI GCNGC 4 cut(s) 172, 294, 606, 750
Sau3AI GATC 2 cut(s) 379, 915
Sau96I GGNCC 2 cut(s) 59, 129
SchI GAGTC 1 cut(s) 61
SfaNI GCATC 1 cut(s) 853
SfcI CTRYAG 2 cut(s) 172, 606
SinI GGWCC 1 cut(s) 129
SmiMI CAYNNNNRTG 2 cut(s) 114, 547
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 4 cut(s) 220, 562, 685, 734
SsiI CCGC 4 cut(s) 127, 405, 612, 924
SspMI CTAG 2 cut(s) 480, 498
StyI CCWWGG 3 cut(s) 62, 115, 305
TaaI ACNGT 5 cut(s) 513, 549, 695, 720, 800
TaiI ACGT 1 cut(s) 20
TasI AATT 4 cut(s) 220, 562, 685, 734
TatI WGTACW 1 cut(s) 697
Tru1I TTAA 4 cut(s) 20, 210, 566, 737
Tru9I TTAA 4 cut(s) 20, 210, 566, 737
TscAI CASTG 4 cut(s) 37, 473, 554, 901
TseFI GTSAC 1 cut(s) 800
TseI GCWGC 4 cut(s) 171, 293, 605, 749
Tsp45I GTSAC 1 cut(s) 800
TspDTI ATGAA 5 cut(s) 88, 248, 666, 780, 807
TspRI CASTG 4 cut(s) 37, 473, 554, 901
VpaK11BI GGWCC 1 cut(s) 129
XagI CCTNNNNNAGG 1 cut(s) 809
XapI RAATTY 1 cut(s) 220
XspI CTAG 2 cut(s) 480, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.