FvH4_5g10170

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003181
Physical Location & Seq
Reverse (-)
5807311 .. 5816176
8866 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10170.t2

Sequence Viewer

Length: 3624 bp
ATGGTTATTTGCTCTTTTCCGGCTCCTTCTGTTGGAAACGGAAACAGTTTGGACAGTCTCCGATTTCAACAAACTCAAATGAATGGGAGAGGGACTGAGAAGCCGAGCAAAGTCGTTATGACGGCGTCTGCATCACAATCGCCGGTGAGGTGGTGGCGCACGGCGTTTCTGACCGTCAGAGACGAATCCCTCACAACTCCTCCTCGCACTCCAATCCCTGACCTCCTCCACAACTTCATCTTCTCCCACTCCCACACTCTCCTCTCCGCCGCGCCCGACCTCCCTCCGCCGGAGGTGACGTCAGATTTGCTGTTCGTGATGGAATTGGTCACCAACAAGCCTCACGGCGCCGGAGACTTGACCTCAACCTTCGCACACACAAGTCACCTGATCCATGACGTCAGCCATCGTCTTCCGCTTGAGTTCAGTTCAGCTTCGTGGACTATCATGCTCGACGGTTTCGGCAAGATGCTTCAATTTTTCATTGCTTCTTCGTCATTTACGCCGATCATGGAATGCTTACAGACTCTGAGGCGTGTGATGAGTACCTACCAGAGGAAGTGTTCTACGGCTGATGAGATTCAGCTGGTGAAGTTCCTTCTGCGCTTAATCGAGAGCTGCCATTCGGAGTTAAGCAGCTCGTCGCATTCGTTACGCAACCAGAGCTCTGCTTCTGAAGTTGGAAAGAGAAAGCCAATGCCGCAATATGGCAGCTTATGGGAAGTGCAGACCCTTGCATTCAATGTGCTTGGAGAAACAATCTCTAGGGCCGGCTCGTTGTTTCCTGTAGATATCTGGAAATCATCTATTGAGGTTTTTAGGAAAGTGATGGATGTATTGGCAGCTAAAAGCCAGCTTGTAGAAGATACTGTAATGTCGAGGTTTTATTTATCTCTCCTGAATTGTCTACACTCGACTCTCGCAGATCGTAAGTGTTCCCTCTCTGACCATGTGTCAGGTTTTGTAGCTGCTTTGCGAATGTTCCTCTCCTATGGTGTCAGTAGTAGAAGCCAGCTTAGCAGGCCGATTACTGGTCAAAAGGAGAGTGAACTTAGTGTAGTGAGCCTTAAGGCTGGTTTGGAAGATCCTAAAAAGACAGATCGTAGTCCATATAGACCTCCACACCTACGCAAAAGGGACAGTTCAAAGCAGATTGGAGCTCGAAATTCTCAAGGTTTATCAGATCAGGAATCTTCCACACTTGACTTCACCTCATCAGATTCAGATTACAGTGACAGTGATGGATCACTTAAAGACACTGAGAGCAATCAGAAATCGAAGGTCAGAGTAGCTGCTATTGTTTGTATACAGGATCTCTGTCAAGCTGATTCAAAATCATTTAGTAGCCAATGGACACTGCTTCTGCCAACCAGTGATGTACTACAACCTAGGAAGTTTGAAGCAACACTAATGACTTGTTTGCTGTTTGATCCCTATTTAAAGGCACGGGTTGCATCAGCCTCAACTCTGGAAGCAATGTTGGATGGGCCTTCTTCTGTTATACTGCAGGTAGCTGAGTTCAGAGAGTCTTCTAAACGGGGATCATTTACAGCACTTTCAAGTTCCCTTGGACATATACTAATGCAGCTTCATACAGGTATTTTATACTTAATTCAGCGAGAAACTCATAATCGATTACTGGCATCCTTGTTCAAGATTCTCATGCTTTTGATATCATCTACACCATACACAAGGATGCCTGGAGAACTGTTGCCAACAGTTTTTACATCTCTACAAGAAAGGATACAAAATGGGTTTCAATATAAAAGTGATCAGACTGGCCTGCTGGCTGCTTCTTTTAGTTGCTTGACAACAGCTCTGAATACCTCACCATCTTCACCACAAATTAAAGAAATGCTTCAGAGAGAGATATTTAATGGTTTTGCTGAGGCCAAAAAGAAGTCGGGTGTTCTTTCTACATTGTTTCAATTTTCAGAACAAGTTAGCAACCCACCGATATGCTTTGAGGCTCTTCAGGCACTTAGGGCTGTGTCACACAATTATCCAAGTATAATGTTTTCCTGTTGGGAACAAATATCCACCACCGTTTATCACCTTTTGAGGGCATCTACTCCTGAAGTTCCTGTAGGACAATGGAAAGGGCATACTGGAAATTCTGTTGGATTTATTGGCGAAAAAATCATTACAGCTGCTATAAGAGTTTTGGATGAATCTCTTCGTGCAATATCTGGATTTAAAGGAACTGAAGACCCTTTAGATGATAAACTACTTGATGCCCCATTTACATCTGACTGCATAAGGATGAAGAAAGTTTCGTCAGCTCCTTCATATGAACTTGAGAATTTTGAAAATACTAGAGATGAACTCACATCATGTCAGTCTGGAATTGAGCAGTGGTGTGAGGCAATTGAGAAGCATATGCCTTTAATTCTACAACATACTTCTGCAATGGTGAGAGCTGCCTCAGTTACCTGTTTTGCGGGTATAACTTCTTCTGTGTTCTGTACACTCTCAAAGGAAAAACAGGAGTTCATTCTTTCGTCTATTGTTCGTGCTGCCGTACACGGTGATGTACCTTCGGTTAGGGCAGCTGCTTGTCGAGCTATTGGTGTCATTTCATGTTTTCCTCAGGTTTCTCAAAGTGCAGAGATCCTTGATAAGTTTGTTCATGCTGTGGAGAGTAACACTCGTGATCCTTTGGTCTCGGTCCGAATAACTGCTTCTTGGGCTTTGGCAAACATATGTGATTCTGTCCATCATTGTATTGATGATTTTAGCTTGGAAAACACTGGAGGCTCTCTTAAAATTTCTCAATTGTTTACCTTATTAAGTGAGTGCGCTTTGCGTCTGACTAAAGATGGAGACAAGATTAAATCAAATGCTGTAAGAGCTCTAGGGAATCTTGCAAGATCTGTCAAATGCACAATTGAATTTGAGACGACAGGGGATTCTGGGAAAGGTTGTCGAAGAGATGTATCAATATCATACCATCCAGCTTCTTTGAGAGATTCGCGTTGGCTTGAGAAGGTTGTTCAGGCATTTATTTCATGTGTTACGACTGGTAATGTCAAGGTCCAATGGAATGTTTGTCATGCTTTGAGCAACCTATTTCTGAACGAGACATTAAGACTGCAAGACATGGATTGGGCTCCATCTGTGTATAGTATTCTTCTACTACTATTACGTGATTCTTCCAACTTTAAGATCAGGATTCAAGCTGCTGCTGCTCTGGCTGTTCCAGCATCGGTTCATGATTATGGGGAATCTTTCTCAGATGTCATCCAGGGTCTGGAGCATATATTAGAGAATCAAGGTTCAAACCAGATTGCATCACCATCAAATTTCAAATATAGGGTAGCTCTTGAAAAGCAGTTAACCTCAACGATATTGCATGTTCTTATCCTCGCTTCAAGCTCTGATCATGAACTGGTGAAAGATTTTCTTGTAAAAAAAGCCTCATTCTTTGAAGACTGGTTCAAGACACTGTGCTCATCGTTAGGAGAGTCAAGTAGTCAACCAGAACTTGAAAATAAGAAGTCCCTTGAGAACCCGAAGAAAGGAATGATATGCAATGCAATTCGGTCACTAGTTCAGCTGTACAATGGCCAGAAGCATCTTGCAATTGCTGAGAAATTTGAGAAGTTAGAGAACAGTATACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4042 HEAT

Protein Analysis

1208

Amino Acids

133.45

Weight (kDa)

7.34

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4042 PF13251 427 - 610 1.8e-51 Domain of unknown function (DUF4042)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 302, 402
Acc36I ACCTGC 1 cut(s) 1498
AccB1I GGYRCC 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 3250
AccI GTMKAC 3 cut(s) 907, 1306, 3616
AccII CGCG 2 cut(s) 272, 2974
AciI CCGC 6 cut(s) 267, 270, 287, 416, 701, 2441
AclWI GGATC 8 cut(s) 385, 1079, 1252, 1320, 1424, 1549, 2605, 2648
AcoI YGGCCR 1 cut(s) 3565
AcsI RAATTY 7 cut(s) 1165, 2113, 2302, 2766, 2891, 3301, 3593
AcuI CTGAAG 5 cut(s) 696, 1844, 1958, 2097, 2226
AcyI GRCGYC 4 cut(s) 125, 299, 348, 399
AfaI GTAC 6 cut(s) 547, 1380, 2467, 2523, 2535, 3560
AfiI CCNNNNNNNGG 8 cut(s) 32, 289, 555, 707, 1031, 2062, 3250, 3518
AflII CTTAAG 1 cut(s) 1067
AhdI GACNNNNNGTC 1 cut(s) 952
AhlI ACTAGT 1 cut(s) 3547
AjnI CCWGG 2 cut(s) 1699, 3243
Alw21I GWGCWC 4 cut(s) 668, 1162, 2854, 3452
Alw26I GTCTC 7 cut(s) 62, 174, 348, 2668, 2817, 2891, 3074
AlwI GGATC 8 cut(s) 385, 1079, 1252, 1320, 1424, 1549, 2605, 2648
AlwNI CAGNNNCTG 2 cut(s) 529, 3250
AoxI GGCC 6 cut(s) 768, 1022, 1487, 1780, 1890, 3565
ApoI RAATTY 7 cut(s) 1165, 2113, 2302, 2766, 2891, 3301, 3593
ArsI GACNNNNNNTTYG 2 cut(s) 289, 321
Asp700I GAANNNNTTC 2 cut(s) 1857, 2175
AspA2I CCTAGG 1 cut(s) 1388
AspLEI GCGC 5 cut(s) 159, 274, 350, 606, 2801
AspS9I GGNCC 4 cut(s) 768, 1487, 2668, 3034
AvaII GGWCC 2 cut(s) 2668, 3034
AvrII CCTAGG 1 cut(s) 1388
AxyI CCTNAGG 1 cut(s) 2589
BalI TGGCCA 1 cut(s) 3567
BanI GGYRCC 1 cut(s) 347
BanII GRGCYC 4 cut(s) 668, 1162, 2854, 3112
BarI GAAGNNNNNNTAC 2 cut(s) 2437, 2469
BauI CACGAG 1 cut(s) 2649
BbsI GAAGAC 4 cut(s) 404, 1521, 2214, 3435
Bbv12I GWGCWC 4 cut(s) 668, 1162, 2854, 3452
BbvCI CCTCAGC 1 cut(s) 1887
BceAI ACGGC 5 cut(s) 138, 177, 361, 585, 2504
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BciT130I CCWGG 2 cut(s) 1701, 3245
BciVI GTATCC 1 cut(s) 1737
BclI TGATCA 2 cut(s) 1771, 3379
BcoDI GTCTC 7 cut(s) 62, 174, 348, 2668, 2817, 2891, 3074
BcuI ACTAGT 1 cut(s) 3547
BfaI CTAG 5 cut(s) 765, 1389, 2316, 2855, 3548
BfmI CTRYAG 3 cut(s) 786, 1505, 2085
BfoI RGCGCY 1 cut(s) 351
BfrI CTTAAG 1 cut(s) 1067
BfuAI ACCTGC 1 cut(s) 1498
BfuI GTATCC 1 cut(s) 1737
BglII AGATCT 1 cut(s) 2870
BlnI CCTAGG 1 cut(s) 1388
BlpI GCTNAGC 1 cut(s) 1016
Bme1390I CCNGG 2 cut(s) 1701, 3245
Bme18I GGWCC 2 cut(s) 2668, 3034
BmeRI GACNNNNNGTC 1 cut(s) 952
BmgT120I GGNCC 4 cut(s) 768, 1487, 2668, 3034
BmiI GGNNCC 3 cut(s) 24, 349, 3111
BmrFI CCNGG 2 cut(s) 1701, 3245
BpiI GAAGAC 4 cut(s) 404, 1521, 2214, 3435
BplI GAGNNNNNCTC 4 cut(s) 2310, 2342, 2632, 2664
BpmI CTGGAG 3 cut(s) 1722, 2772, 3272
Bpu10I CCTNAGC 1 cut(s) 1887
Bpu1102I GCTNAGC 1 cut(s) 1016
BpuEI CTTGAG 5 cut(s) 440, 1155, 2318, 3002, 3524
Bsa29I ATCGAT 1 cut(s) 1633
BsaAI YACGTR 1 cut(s) 3146
BsaHI GRCGYC 4 cut(s) 125, 299, 348, 399
BsaI GGTCTC 1 cut(s) 2668
BsaJI CCNNGG 3 cut(s) 1388, 1567, 3244
BsaXI ACNNNNNCTCC 4 cut(s) 184, 214, 284, 314
Bsc4I CCNNNNNNNGG 8 cut(s) 32, 289, 555, 707, 1031, 2062, 3250, 3518
Bse118I RCCGGY 2 cut(s) 142, 770
Bse1I ACTGG 9 cut(s) 1036, 1371, 1644, 1783, 2113, 2755, 3025, 3393, 3437
Bse21I CCTNAGG 1 cut(s) 2589
Bse3DI GCAATG 4 cut(s) 483, 1482, 2415, 3538
BseBI CCWGG 2 cut(s) 1701, 3245
BseCI ATCGAT 1 cut(s) 1633
BseDI CCNNGG 3 cut(s) 1388, 1567, 3244
BseGI GGATG 8 cut(s) 838, 1489, 1643, 1701, 2173, 2268, 2950, 3240
BseLI CCNNNNNNNGG 8 cut(s) 32, 289, 555, 707, 1031, 2062, 3250, 3518
BseMI GCAATG 4 cut(s) 483, 1482, 2415, 3538
BseMII CTCAG 9 cut(s) 87, 521, 1251, 1506, 1878, 2439, 2603, 3246, 3579
BseNI ACTGG 9 cut(s) 1036, 1371, 1644, 1783, 2113, 2755, 3025, 3393, 3437
BseRI GAGGAG 4 cut(s) 189, 192, 215, 251
BsgI GTGCAG 2 cut(s) 746, 2625
Bsh1236I CGCG 2 cut(s) 272, 2974
BshFI GGCC 6 cut(s) 770, 1024, 1489, 1782, 1892, 3567
BshNI GGYRCC 1 cut(s) 347
BshVI ATCGAT 1 cut(s) 1633
BsiHKAI GWGCWC 4 cut(s) 668, 1162, 2854, 3452
BsiSI CCGG 5 cut(s) 20, 143, 290, 351, 771
BslFI GGGAC 3 cut(s) 106, 1151, 3484
BslI CCNNNNNNNGG 8 cut(s) 32, 289, 555, 707, 1031, 2062, 3250, 3518
BsmAI GTCTC 7 cut(s) 62, 174, 348, 2668, 2817, 2891, 3074
BsmBI CGTCTC 2 cut(s) 174, 2891
BsmFI GGGAC 3 cut(s) 106, 1151, 3484
BsmI GAATGC 3 cut(s) 521, 646, 737
BsnI GGCC 6 cut(s) 770, 1024, 1489, 1782, 1892, 3567
Bso31I GGTCTC 1 cut(s) 2668
Bsp1286I GDGCHC 5 cut(s) 668, 1162, 2854, 3112, 3452
Bsp1407I TGTACA 2 cut(s) 2465, 3558
Bsp1720I GCTNAGC 1 cut(s) 1016
BspACI CCGC 6 cut(s) 267, 270, 287, 416, 701, 2441
BspANI GGCC 6 cut(s) 770, 1024, 1489, 1782, 1892, 3567
BspCNI CTCAG 9 cut(s) 88, 522, 1252, 1507, 1879, 2438, 2602, 3245, 3580
BspDI ATCGAT 1 cut(s) 1633
BspFNI CGCG 2 cut(s) 272, 2974
BspHI TCATGA 2 cut(s) 3211, 3382
BspLI GGNNCC 3 cut(s) 24, 349, 3111
BspMAI CTGCAG 1 cut(s) 1509
BspMI ACCTGC 1 cut(s) 1498
BspPI GGATC 8 cut(s) 385, 1079, 1252, 1320, 1424, 1549, 2605, 2648
BspQI GCTCTTC 1 cut(s) 1977
BspT107I GGYRCC 1 cut(s) 347
BspTI CTTAAG 1 cut(s) 1067
BspTNI GGTCTC 1 cut(s) 2668
BsrDI GCAATG 4 cut(s) 483, 1482, 2415, 3538
BsrFI RCCGGY 2 cut(s) 142, 770
BsrGI TGTACA 2 cut(s) 2465, 3558
BsrI ACTGG 9 cut(s) 1036, 1371, 1644, 1783, 2113, 2755, 3025, 3393, 3437
BssAI RCCGGY 2 cut(s) 142, 770
BssECI CCNNGG 3 cut(s) 1388, 1567, 3244
BssNAI GTATAC 2 cut(s) 1307, 3617
BssNI GRCGYC 4 cut(s) 125, 299, 348, 399
BssSI CACGAG 1 cut(s) 2649
BssT1I CCWWGG 2 cut(s) 1388, 1567
Bst1107I GTATAC 2 cut(s) 1307, 3617
Bst2BI CACGAG 1 cut(s) 2649
Bst2UI CCWGG 2 cut(s) 1701, 3245
Bst6I CTCTTC 3 cut(s) 1977, 2181, 2923
BstACI GRCGYC 4 cut(s) 125, 299, 348, 399
BstAFI CTTAAG 1 cut(s) 1067
BstAPI GCANNNNNTGC 1 cut(s) 1451
BstAUI TGTACA 2 cut(s) 2465, 3558
BstBAI YACGTR 1 cut(s) 3146
BstC8I GCNNGC 6 cut(s) 772, 854, 1013, 1022, 1784, 1788
BstEII GGTNACC 1 cut(s) 328
BstENI CCTNNNNNAGG 1 cut(s) 553
BstF5I GGATG 8 cut(s) 838, 1489, 1643, 1701, 2173, 2268, 2950, 3240
BstFNI CGCG 2 cut(s) 272, 2974
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 5 cut(s) 159, 274, 350, 606, 2801
BstMAI GTCTC 7 cut(s) 62, 174, 348, 2668, 2817, 2891, 3074
BstNI CCWGG 2 cut(s) 1701, 3245
BstNSI RCATGY 1 cut(s) 3356
BstPI GGTNACC 1 cut(s) 328
BstSCI CCNGG 2 cut(s) 1699, 3243
BstSFI CTRYAG 3 cut(s) 786, 1505, 2085
BstUI CGCG 2 cut(s) 272, 2974
BstV2I GAAGAC 4 cut(s) 404, 1521, 2214, 3435
BstX2I RGATCY 4 cut(s) 1084, 1312, 2610, 2870
BstYI RGATCY 4 cut(s) 1084, 1312, 2610, 2870
BstZ17I GTATAC 2 cut(s) 1307, 3617
Bsu15I ATCGAT 1 cut(s) 1633
Bsu36I CCTNAGG 1 cut(s) 2589
BsuI GTATCC 1 cut(s) 1737
BsuRI GGCC 6 cut(s) 770, 1024, 1489, 1782, 1892, 3567
BsuTUI ATCGAT 1 cut(s) 1633
BtsCI GGATG 8 cut(s) 838, 1489, 1643, 1701, 2173, 2268, 2950, 3240
BtsI GCAGTG 2 cut(s) 1355, 2360
BtsIMutI CAGTG 8 cut(s) 1237, 1243, 1257, 1355, 1378, 2360, 2748, 3443
BveI ACCTGC 1 cut(s) 1498
Cac8I GCNNGC 6 cut(s) 772, 854, 1013, 1022, 1784, 1788
CaiI CAGNNNCTG 2 cut(s) 529, 3250
CciI TCATGA 2 cut(s) 3211, 3382
CfoI GCGC 5 cut(s) 159, 274, 350, 606, 2801
Cfr10I RCCGGY 2 cut(s) 142, 770
Cfr13I GGNCC 4 cut(s) 768, 1487, 2668, 3034
ClaI ATCGAT 1 cut(s) 1633
CpoI CGGWCCG 1 cut(s) 2668
CseI GACGC 2 cut(s) 114, 2795
Csp6I GTAC 6 cut(s) 546, 1379, 2466, 2522, 2534, 3559
CspI CGGWCCG 1 cut(s) 2668
CviQI GTAC 6 cut(s) 546, 1379, 2466, 2522, 2534, 3559
DinI GGCGCC 1 cut(s) 349
DraI TTTAAA 2 cut(s) 1440, 2197
DriI GACNNNNNGTC 1 cut(s) 952
EaeI YGGCCR 1 cut(s) 3565
Eam1104I CTCTTC 3 cut(s) 1977, 2181, 2923
Eam1105I GACNNNNNGTC 1 cut(s) 952
EarI CTCTTC 3 cut(s) 1977, 2181, 2923
EciI GGCGGA 2 cut(s) 256, 276
Ecl136II GAGCTC 3 cut(s) 666, 1160, 2852
Eco130I CCWWGG 2 cut(s) 1388, 1567
Eco24I GRGCYC 4 cut(s) 668, 1162, 2854, 3112
Eco31I GGTCTC 1 cut(s) 2668
Eco32I GATATC 2 cut(s) 793, 1674
Eco47I GGWCC 2 cut(s) 2668, 3034
Eco53kI GAGCTC 3 cut(s) 666, 1160, 2852
Eco57I CTGAAG 5 cut(s) 696, 1844, 1958, 2097, 2226
Eco81I CCTNAGG 1 cut(s) 2589
Eco91I GGTNACC 1 cut(s) 328
EcoICRI GAGCTC 3 cut(s) 666, 1160, 2852
EcoNI CCTNNNNNAGG 1 cut(s) 553
EcoO65I GGTNACC 1 cut(s) 328
EcoRII CCWGG 2 cut(s) 1699, 3243
EcoRV GATATC 2 cut(s) 793, 1674
EcoT14I CCWWGG 2 cut(s) 1388, 1567
EcoT38I GRGCYC 4 cut(s) 668, 1162, 2854, 3112
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
ErhI CCWWGG 2 cut(s) 1388, 1567
Esp3I CGTCTC 2 cut(s) 174, 2891
FalI AAGNNNNNCTT 4 cut(s) 1842, 1874, 3387, 3419
FaqI GGGAC 3 cut(s) 106, 1151, 3484
FauI CCCGC 1 cut(s) 2434
FauNDI CATATG 3 cut(s) 2290, 2379, 2702
FbaI TGATCA 2 cut(s) 1771, 3379
FblI GTMKAC 3 cut(s) 907, 1306, 3616
FokI GGATG 8 cut(s) 845, 1496, 1630, 1708, 2180, 2275, 2937, 3227
FriOI GRGCYC 4 cut(s) 668, 1162, 2854, 3112
FspBI CTAG 5 cut(s) 765, 1389, 2316, 2855, 3548
GlaI GCGC 5 cut(s) 158, 273, 349, 605, 2800
GsuI CTGGAG 3 cut(s) 1722, 2772, 3272
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 6 cut(s) 770, 1024, 1489, 1782, 1892, 3567
HapII CCGG 5 cut(s) 20, 143, 290, 351, 771
HgaI GACGC 2 cut(s) 114, 2795
HhaI GCGC 5 cut(s) 159, 274, 350, 606, 2801
Hin1I GRCGYC 4 cut(s) 125, 299, 348, 399
Hin6I GCGC 5 cut(s) 157, 272, 348, 604, 2799
HinP1I GCGC 5 cut(s) 157, 272, 348, 604, 2799
HincII GTYRAC 2 cut(s) 3336, 3476
HindII GTYRAC 2 cut(s) 3336, 3476
HpaI GTTAAC 1 cut(s) 3336
HpaII CCGG 5 cut(s) 20, 143, 290, 351, 771
Hpy99I CGWCG 2 cut(s) 458, 646
HpyAV CCTTC 8 cut(s) 36, 379, 608, 1273, 1500, 2295, 2547, 2980
HpyCH4IV ACGT 3 cut(s) 299, 399, 3145
HpySE526I ACGT 3 cut(s) 299, 399, 3145
Hsp92I GRCGYC 4 cut(s) 125, 299, 348, 399
HspAI GCGC 5 cut(s) 157, 272, 348, 604, 2799
KasI GGCGCC 1 cut(s) 347
KroI GCCGGC 1 cut(s) 770
KroNI GCCGGC 1 cut(s) 772
Ksp22I TGATCA 2 cut(s) 1771, 3379
KspAI GTTAAC 1 cut(s) 3336
LguI GCTCTTC 1 cut(s) 1977
LmnI GCTCC 5 cut(s) 28, 1157, 2287, 3115, 3253
MaeI CTAG 5 cut(s) 765, 1389, 2316, 2855, 3548
MaeII ACGT 3 cut(s) 299, 399, 3145
MfeI CAATTG 4 cut(s) 2367, 2774, 2886, 3582
MflI RGATCY 4 cut(s) 1084, 1312, 2610, 2870
MhlI GDGCHC 5 cut(s) 668, 1162, 2854, 3112, 3452
MlsI TGGCCA 1 cut(s) 3567
MluNI TGGCCA 1 cut(s) 3567
Mly113I GGCGCC 1 cut(s) 348
MlyI GAGTC 4 cut(s) 520, 910, 1535, 3473
MmeI TCCRAC 5 cut(s) 13, 661, 1461, 2101, 3180
Mox20I TGGCCA 1 cut(s) 3567
MroNI GCCGGC 1 cut(s) 770
MroXI GAANNNNTTC 2 cut(s) 1857, 2175
MscI TGGCCA 1 cut(s) 3567
MslI CAYNNNNRTG 5 cut(s) 1694, 1957, 2261, 2529, 3216
Msp20I TGGCCA 1 cut(s) 3567
MspA1I CMGCKG 4 cut(s) 586, 2150, 2552, 3556
MspCI CTTAAG 1 cut(s) 1067
MspI CCGG 5 cut(s) 20, 143, 290, 351, 771
MspR9I CCNGG 2 cut(s) 1701, 3245
MunI CAATTG 4 cut(s) 2367, 2774, 2886, 3582
Mva1269I GAATGC 3 cut(s) 521, 646, 737
MvaI CCWGG 2 cut(s) 1701, 3245
MvnI CGCG 2 cut(s) 272, 2974
NaeI GCCGGC 1 cut(s) 772
NarI GGCGCC 1 cut(s) 348
NdeI CATATG 3 cut(s) 2290, 2379, 2702
NgoMIV GCCGGC 1 cut(s) 770
NlaIV GGNNCC 3 cut(s) 24, 349, 3111
NmeAIII GCCGAG 1 cut(s) 129
NmuCI GTSAC 6 cut(s) 295, 328, 383, 1232, 1992, 3543
NspI RCATGY 1 cut(s) 3356
PagI TCATGA 2 cut(s) 3211, 3382
PciSI GCTCTTC 1 cut(s) 1977
PcsI WCGNNNNNNNCGW 1 cut(s) 647
PctI GAATGC 3 cut(s) 521, 646, 737
PdiI GCCGGC 1 cut(s) 772
PdmI GAANNNNTTC 2 cut(s) 1857, 2175
PflFI GACNNNGTC 1 cut(s) 124
PflMI CCANNNNNTGG 1 cut(s) 3250
PleI GAGTC 4 cut(s) 520, 910, 1534, 3472
PluTI GGCGCC 1 cut(s) 351
PpsI GAGTC 4 cut(s) 520, 910, 1534, 3472
Ppu21I YACGTR 1 cut(s) 3146
Psp124BI GAGCTC 3 cut(s) 668, 1162, 2854
Psp6I CCWGG 2 cut(s) 1699, 3243
PspEI GGTNACC 1 cut(s) 328
PspGI CCWGG 2 cut(s) 1699, 3243
PspN4I GGNNCC 3 cut(s) 24, 349, 3111
PspPI GGNCC 4 cut(s) 768, 1487, 2668, 3034
PstI CTGCAG 1 cut(s) 1509
PstNI CAGNNNCTG 2 cut(s) 529, 3250
PsuI RGATCY 4 cut(s) 1084, 1312, 2610, 2870
PsyI GACNNNGTC 1 cut(s) 124
PvuII CAGCTG 4 cut(s) 586, 2150, 2552, 3556
RsaI GTAC 6 cut(s) 547, 1380, 2467, 2523, 2535, 3560
RsaNI GTAC 6 cut(s) 546, 1379, 2466, 2522, 2534, 3559
RseI CAYNNNNRTG 5 cut(s) 1694, 1957, 2261, 2529, 3216
Rsr2I CGGWCCG 1 cut(s) 2668
RsrII CGGWCCG 1 cut(s) 2668
SacI GAGCTC 3 cut(s) 668, 1162, 2854
SapI GCTCTTC 1 cut(s) 1977
Sau96I GGNCC 4 cut(s) 768, 1487, 2668, 3034
SchI GAGTC 4 cut(s) 520, 910, 1535, 3473
ScrFI CCNGG 2 cut(s) 1701, 3245
SduI GDGCHC 5 cut(s) 668, 1162, 2854, 3112, 3452
SfcI CTRYAG 3 cut(s) 786, 1505, 2085
SfoI GGCGCC 1 cut(s) 349
SgrAI CRCCGGYG 1 cut(s) 142
SinI GGWCC 2 cut(s) 2668, 3034
SmiMI CAYNNNNRTG 5 cut(s) 1694, 1957, 2261, 2529, 3216
SmlI CTYRAG 6 cut(s) 419, 1067, 1170, 2297, 2981, 3503
SmoI CTYRAG 6 cut(s) 419, 1067, 1170, 2297, 2981, 3503
SpeI ACTAGT 1 cut(s) 3547
SsiI CCGC 6 cut(s) 267, 270, 287, 416, 701, 2441
SspDI GGCGCC 1 cut(s) 347
SspMI CTAG 5 cut(s) 765, 1389, 2316, 2855, 3548
SstI GAGCTC 3 cut(s) 668, 1162, 2854
StyD4I CCNGG 2 cut(s) 1699, 3243
StyI CCWWGG 2 cut(s) 1388, 1567
TaiI ACGT 3 cut(s) 302, 402, 3148
TaqI TCGA 9 cut(s) 453, 612, 878, 914, 1162, 1277, 1633, 2560, 2926
TaqII GACCGA 2 cut(s) 2656, 3531
TatI WGTACW 3 cut(s) 1378, 2465, 3558
TauI GCSGC 2 cut(s) 272, 703
TscAI CASTG 8 cut(s) 1237, 1243, 1264, 1362, 1378, 2360, 2755, 3450
TseFI GTSAC 6 cut(s) 295, 328, 383, 1232, 1992, 3543
Tsp45I GTSAC 6 cut(s) 295, 328, 383, 1232, 1992, 3543
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 8 cut(s) 1237, 1243, 1264, 1362, 1378, 2360, 2755, 3450
Tth111I GACNNNGTC 1 cut(s) 124
Van91I CCANNNNNTGG 1 cut(s) 3250
Vha464I CTTAAG 1 cut(s) 1067
VpaK11BI GGWCC 2 cut(s) 2668, 3034
XagI CCTNNNNNAGG 1 cut(s) 553
XapI RAATTY 7 cut(s) 1165, 2113, 2302, 2766, 2891, 3301, 3593
XceI RCATGY 1 cut(s) 3356
XmaJI CCTAGG 1 cut(s) 1388
XmiI GTMKAC 3 cut(s) 907, 1306, 3616
XmnI GAANNNNTTC 2 cut(s) 1857, 2175
XspI CTAG 5 cut(s) 765, 1389, 2316, 2855, 3548
ZraI GACGTC 2 cut(s) 300, 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.