Rroxscaffold_3G00266750

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003181
Physical Location & Seq
Reverse (-)
59942557 .. 59951568
9012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266750.1

Sequence Viewer

Length: 3507 bp
ATGACGGCGTCTGTATCACAATCGCCGGTGAAGTGGTGGCGGACGGCGTTTCTGACGGTGAGAGACGAAACCCTCACGACTCCTCCTCGCACTCCAATCCCCGAACTCCTCCACAGTTTCATCTTGTCCCACTCCAACACTCTCCTTTCCGCCGCCCCCGACCTCCCTCCGCAGGAGGTGACGTCAGATTTGTTGTTCCTAACGGAATTGATCACCACCAAGTCTCACGGCGCCGGCGACTTGACCCCTACCTTCGCTCACATAATTCACCTGATCCATGACGTCAGCCATCGTCTTCCTCTGGAAGTCAATTCAGCTTCGTGGACTCTCATGCTTGACGGTTTCGGCAACATGCTTCAATTTTTCATTGCTTCTTCGTCATTTACGCCCAGCATGGAATGCTTACAGACTCTGAGGCGTATTATGAGCACCTACCAGAGGAAGTGTTCTCCGGCGGATGAGATTCAGCTGGTGAAGTTCCTTCTGCGCGTAATTGAGAGCTGTCATTCGGAGTTAAGTAGCTCATCGCATTCGATTCGCAACCAGAGCTCTGCTTCTGAAGTTGGAAAGAGAAAGCCATTGCCGCGACATGGCAGCTTATGGGAAGTTCAGAGCCTTGCATTCAATATGCTTGGAGAAACAATCTCTAGGGCCGGCTCGTTGTTTCCCGTGGATATCTGGAAATCATCTGTTGAGGTTTTTAGGAAAGTGATGGATGTATTGGCAGCTAAAAGCCTGCTTGTAGAAGACACTGTAATGTCGAGGTTTTATTTATCTCTCCTGCATTGTCTGCACTTGACTCTCTCAGATCGTAAGTGTTCCCTCTCTGACCATGTGTCAGGTTTTGTAGCTGCTTTGCGAATGTTCTTGTCCTATGGTCTTAGTAGTAGAAGCCAGCTTACCAGGCCGGCAACTGGTCAAAAGGAGAGTGAACTTAGCGTAGCCAGCCTTAAGTCTGGTTTGGAAGATCCTAAAAAGACAGATCGTAGTCCATATAGACCTCCACACCTACGCAAAAGGGACAGTTCAAAGCAGATTGGAGCTCGAGGTTCTCAAGGTTTATCAGATCAGGAATCTTCCACACTTGACTTTGCCTCATCAGATTCAGATTATAGTGACAGTGATGGATCACTTAAAGACACTGAAAGCATTCAGAAATCGAAGGTCAGAGTAGCTGCCATTGTTTGTATACAGGATCTCTGTCAAGCTGATTCGAAATCATTTAGTAGCCAATGGACACTGCTTTTGCCAACCAGTGATGTACTACAACCTAGGAAGTTTGAAGCAACACTAATGACTTGTTTGCTGTTTGATCCCTATTTGAAGGCACGGGTTGCATCTGCCTCAACTCTGGAAGCAATGTTGGATGGGCCTTCTTCTGTTTTTCTGCAGGTAGCAGAGTTCAAAGAGTCTTCTAAAAGGGGATCTTTTACAGCACTTTCAAGTTCCCTTGGACATATACTAATGCAGCTTCATACAGGTATTTTATACGTAATTCAACGGGAAACTCATAGTAGATTGCTGGCATCCTTGTTCAAGATTCTCATGCTTTTGATATCATCTACACCATATTCAAGAATGCCCGGAGAATTGTTGCCAACAGTTTTTACATCTCTACAAGAAAGGATACAAAATGGGTTTCAGTTTAAAAGTGATCAGACTGGCCTGCTGGCTTCTTCTTTTAGTTGCTTGACTACAGCTCTGAATACCTCACCATCTTCACCGCAAATCAAAGAAATGCTTCAGAGAGAGATATTTAATGGTTTTGCCAAGGCCAAAAAGAAGTCGGGTGTTCTTTTTACGTTGTTTCAATTTTCAGAACAAGTTAGCAACCCGCCAATATGCTTCGAGGCTCTTCAGGCGCTTAGGGCTGTGTCGCACAATTACCCCAGTATAATGTTTTCCTGTTGGGAACAAATTTCCACCATTGTTTATCACCTTTTGAGGGCAGCTACTCCTGAAGTTCCTGCAGGACAATGGAAAGGGCATACTGGAAATTCCGTTGGATTCATTGGGGAAAAACTCATTACAGCTGCTATTAGAGTTTTGGATGAATCTCTTCGTGCAATATCTGGATTTAAAGGAACTGAAGACCCTTTAGATGATAAACTACTTGATGCTCCATTTACTTCTGACTGCATAAGGATGAAGAAAGTTTCATCAGCTCCTTCATATGAACTAGAGAGCTTGGAAAATACTAGAGATGAACCCACATCATGTCAGTCTGGAATTGAGCAGTGGTGTGAAGCAATTGAGAAGCATATGCCTTTAATTCTACAACATACTTCTGCAATGGTGAGAGCTGCATCAGTTACCTGTTTTGCGGGTATAACTTCTTCTGTGTTCTGCACACTCTCGAAGGAAAAACGGGACTTCATTCTTTCATCTTTAGTTCGTCCTGCCGTACTCGGTGATGTACCTTCAGTTAGGGCAGCTGCTTGTCGGGCCATTGGTGTCATTTCATGTTTTCCTCAAGTTTCTCAAAGTGCAGAGATCCTTGATAAGTTTGTTCGTGCTGTGGAGAGTAACACTCGTGATCCTTTGGTCTCGGTCCGAATAACTGCTTCTTGGGCTTTGGCAAACATATGTGATTCTATCCATCATTGTATTGATGATTTTAGCTTGGAAAAGACTGAAGGCTCTCTTAAAATTTCTCAATTGTTTACCTTATTAACCGAGTGCGCTTTGCGTCTGACTAAGGATGGAGACAAGATTAAATCAAATGCTGTAAGAGCTCTAGGGAATCTTTCAAGATCTATCAAATGTACAATTGAATTTGAGGCGCCAGGGGATTATGGGAAAGGTTGTCAAGGAGATGTCTCAATATCATACCATCCAGCTTCTTTGAGAGATTCCCGTTGGCTTGAGAGGGTTGTTCAGGCATTTATTTCTTGTGTTACGACTGGTAATGTCAAGGTCCAATGGAATGTTTGTCATGCTTTGAGCAACCTATTTCTGAATGAGACATTAAGACTGCAAGACATGGATTGGGCTCCATCTGTGTTTAGTATTCTTCTACTACTGTTACGTGATTCTTCCAACTTTAAGATAAGAATCCAAGCAGCTGCTGCTCTGGCTGTTCCAGCATCGGTGCGTGATTATGGGGAATCTTTCTCAGACGTCATCCAGGGTCTGGAGCATATATTAGAGAATCAAGGTTCAGACCAGATTGCATCACCATCAAATTTCAAATATAGGGTTGCCCTTGAAAAGCAGTTAACCTCAACCATGTTGCATGTTCTCATCCTCGCTTCAAGCTCTGATCATGAACCAGTGAAAGTTTTTCTTGTAAAAAAAGCCTCATTCTTTGAAGACTGGTTCAAGGCACTGTGCTCATCACTAGGAGAGTCGAGTAGTCAACCGGAACTTGAAAATAAGAAGTCCCTTGAGAACCCAAAGAAAGAGATGATATGCAATGCAATTCGGTCATTAATTCAGCTGTACAATGACAGGAAGCATCATGCAATTGCCGAGAAATTTGAGAAGTTAGAGAAAAGCATACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4042 HEAT

Protein Analysis

1168

Amino Acids

129.33

Weight (kDa)

7.53

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4042 PF13251 388 - 571 4.4e-52 Domain of unknown function (DUF4042)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 185, 285, 3123
Acc36I ACCTGC 1 cut(s) 1381
AccB1I GGYRCC 2 cut(s) 230, 2782
AccB7I CCANNNNNTGG 1 cut(s) 3133
AccI GTMKAC 1 cut(s) 1189
AccII CGCG 2 cut(s) 489, 586
AciI CCGC 9 cut(s) 40, 150, 153, 170, 455, 584, 1724, 1835, 2324
AclWI GGATC 8 cut(s) 268, 962, 1135, 1203, 1307, 1432, 2488, 2531
AcsI RAATTY 6 cut(s) 1917, 1996, 2649, 2774, 3184, 3476
AcuI CTGAAG 7 cut(s) 579, 1727, 1841, 1980, 2109, 2406, 2655
AcyI GRCGYC 6 cut(s) 8, 182, 231, 282, 2783, 3120
AfaI GTAC 5 cut(s) 1263, 2406, 2418, 2767, 3443
AfiI CCNNNNNNNGG 6 cut(s) 172, 438, 590, 914, 1945, 3133
AflII CTTAAG 1 cut(s) 950
AhdI GACNNNNNGTC 1 cut(s) 835
AjnI CCWGG 3 cut(s) 902, 2785, 3126
Alw21I GWGCWC 5 cut(s) 431, 551, 1045, 2737, 3335
Alw26I GTCTC 6 cut(s) 57, 228, 2551, 2700, 2824, 2957
AlwI GGATC 8 cut(s) 268, 962, 1135, 1203, 1307, 1432, 2488, 2531
AlwNI CAGNNNCTG 3 cut(s) 412, 3068, 3133
Ama87I CYCGRG 1 cut(s) 1044
AoxI GGCC 6 cut(s) 651, 905, 1370, 1663, 1773, 2445
ApoI RAATTY 6 cut(s) 1917, 1996, 2649, 2774, 3184, 3476
ArsI GACNNNNNNTTYG 4 cut(s) 172, 204, 1228, 1260
AseI ATTAAT 1 cut(s) 3431
Asp700I GAANNNNTTC 3 cut(s) 1149, 1740, 2058
AspA2I CCTAGG 1 cut(s) 1271
AspLEI GCGC 5 cut(s) 233, 489, 1864, 2684, 2785
AspS9I GGNCC 5 cut(s) 651, 1370, 2445, 2551, 2917
AsuC2I CCSGG 1 cut(s) 1584
AsuII TTCGAA 1 cut(s) 1214
AvaI CYCGRG 1 cut(s) 1044
AvaII GGWCC 2 cut(s) 2551, 2917
AvrII CCTAGG 1 cut(s) 1271
BanI GGYRCC 2 cut(s) 230, 2782
BanII GRGCYC 4 cut(s) 551, 1045, 2737, 2995
BarI GAAGNNNNNNTAC 4 cut(s) 883, 915, 2320, 2352
BauI CACGAG 1 cut(s) 2532
BbsI GAAGAC 5 cut(s) 287, 753, 1404, 2097, 3318
Bbv12I GWGCWC 5 cut(s) 431, 551, 1045, 2737, 3335
BceAI ACGGC 4 cut(s) 21, 60, 244, 2387
BcgI CGANNNNNNTGC 2 cut(s) 518, 552
BciT130I CCWGG 3 cut(s) 904, 2787, 3128
BciVI GTATCC 1 cut(s) 1620
BclI TGATCA 3 cut(s) 210, 1654, 3262
BcnI CCSGG 1 cut(s) 1584
BcoDI GTCTC 6 cut(s) 57, 228, 2551, 2700, 2824, 2957
BfaI CTAG 6 cut(s) 648, 1272, 2180, 2199, 2738, 3341
BfmI CTRYAG 3 cut(s) 1388, 1695, 1968
BfoI RGCGCY 3 cut(s) 234, 1865, 2786
BfrI CTTAAG 1 cut(s) 950
BfuAI ACCTGC 1 cut(s) 1381
BfuI GTATCC 1 cut(s) 1620
BglII AGATCT 1 cut(s) 2753
BlnI CCTAGG 1 cut(s) 1271
Bme1390I CCNGG 4 cut(s) 904, 1584, 2787, 3128
Bme18I GGWCC 2 cut(s) 2551, 2917
BmeRI GACNNNNNGTC 1 cut(s) 835
BmeT110I CYCGRG 1 cut(s) 1044
BmgT120I GGNCC 5 cut(s) 651, 1370, 2445, 2551, 2917
BmiI GGNNCC 3 cut(s) 232, 2784, 2994
BmrFI CCNGG 4 cut(s) 904, 1584, 2787, 3128
BmrI ACTGGG 1 cut(s) 1884
BmsI GCATC 7 cut(s) 1346, 1535, 2107, 2315, 3095, 3182, 3466
BmuI ACTGGG 1 cut(s) 1884
BpiI GAAGAC 5 cut(s) 287, 753, 1404, 2097, 3318
BplI GAGNNNNNCTC 2 cut(s) 2515, 2547
BpmI CTGGAG 1 cut(s) 3155
Bpu10I CCTNAGC 1 cut(s) 1865
Bpu14I TTCGAA 1 cut(s) 1214
BpuEI CTTGAG 4 cut(s) 1038, 2457, 2885, 3407
BpuMI CCSGG 1 cut(s) 1584
BsaAI YACGTR 2 cut(s) 1492, 3029
BsaBI GATNNNNATC 1 cut(s) 3053
BsaHI GRCGYC 6 cut(s) 8, 182, 231, 282, 2783, 3120
BsaI GGTCTC 1 cut(s) 2551
BsaJI CCNNGG 6 cut(s) 669, 1271, 1450, 1770, 2786, 3127
BsaWI WCCGGW 1 cut(s) 3361
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 167, 197
Bsc4I CCNNNNNNNGG 6 cut(s) 172, 438, 590, 914, 1945, 3133
Bse118I RCCGGY 4 cut(s) 25, 233, 653, 907
Bse1I ACTGG 8 cut(s) 919, 1254, 1666, 1890, 1996, 2908, 3272, 3320
Bse3DI GCAATG 5 cut(s) 366, 578, 1365, 2298, 3421
Bse8I GATNNNNATC 1 cut(s) 3053
BseBI CCWGG 3 cut(s) 904, 2787, 3128
BseDI CCNNGG 6 cut(s) 669, 1271, 1450, 1770, 2786, 3127
BseJI GATNNNNATC 1 cut(s) 3053
BseLI CCNNNNNNNGG 6 cut(s) 172, 438, 590, 914, 1945, 3133
BseMI GCAATG 5 cut(s) 366, 578, 1365, 2298, 3421
BseMII CTCAG 3 cut(s) 404, 819, 3129
BseNI ACTGG 8 cut(s) 919, 1254, 1666, 1890, 1996, 2908, 3272, 3320
BseRI GAGGAG 3 cut(s) 72, 75, 98
BseYI CCCAGC 1 cut(s) 389
BsgI GTGCAG 3 cut(s) 776, 2332, 2508
Bsh1236I CGCG 2 cut(s) 489, 586
BshFI GGCC 6 cut(s) 653, 907, 1372, 1665, 1775, 2447
BshNI GGYRCC 2 cut(s) 230, 2782
BsiHKAI GWGCWC 5 cut(s) 431, 551, 1045, 2737, 3335
BsiHKCI CYCGRG 1 cut(s) 1044
BsiSI CCGG 7 cut(s) 26, 234, 452, 654, 908, 1584, 3362
BslFI GGGAC 4 cut(s) 112, 1034, 2384, 3367
BslI CCNNNNNNNGG 6 cut(s) 172, 438, 590, 914, 1945, 3133
BsmAI GTCTC 6 cut(s) 57, 228, 2551, 2700, 2824, 2957
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 4 cut(s) 112, 1034, 2384, 3367
BsmI GAATGC 5 cut(s) 404, 529, 620, 1149, 1584
BsnI GGCC 6 cut(s) 653, 907, 1372, 1665, 1775, 2447
Bso31I GGTCTC 1 cut(s) 2551
BsoBI CYCGRG 1 cut(s) 1044
Bsp119I TTCGAA 1 cut(s) 1214
Bsp1286I GDGCHC 6 cut(s) 431, 551, 1045, 2737, 2995, 3335
Bsp1407I TGTACA 2 cut(s) 2765, 3441
BspACI CCGC 9 cut(s) 40, 150, 153, 170, 455, 584, 1724, 1835, 2324
BspANI GGCC 6 cut(s) 653, 907, 1372, 1665, 1775, 2447
BspCNI CTCAG 3 cut(s) 405, 818, 3128
BspFNI CGCG 2 cut(s) 489, 586
BspHI TCATGA 1 cut(s) 3265
BspLI GGNNCC 3 cut(s) 232, 2784, 2994
BspMAI CTGCAG 2 cut(s) 1392, 1972
BspMI ACCTGC 1 cut(s) 1381
BspPI GGATC 8 cut(s) 268, 962, 1135, 1203, 1307, 1432, 2488, 2531
BspQI GCTCTTC 1 cut(s) 1860
BspT104I TTCGAA 1 cut(s) 1214
BspT107I GGYRCC 2 cut(s) 230, 2782
BspTI CTTAAG 1 cut(s) 950
BspTNI GGTCTC 1 cut(s) 2551
BsrDI GCAATG 5 cut(s) 366, 578, 1365, 2298, 3421
BsrFI RCCGGY 4 cut(s) 25, 233, 653, 907
BsrGI TGTACA 2 cut(s) 2765, 3441
BsrI ACTGG 8 cut(s) 919, 1254, 1666, 1890, 1996, 2908, 3272, 3320
BssAI RCCGGY 4 cut(s) 25, 233, 653, 907
BssECI CCNNGG 6 cut(s) 669, 1271, 1450, 1770, 2786, 3127
BssNAI GTATAC 1 cut(s) 1190
BssNI GRCGYC 6 cut(s) 8, 182, 231, 282, 2783, 3120
BssSI CACGAG 1 cut(s) 2532
BssT1I CCWWGG 3 cut(s) 1271, 1450, 1770
Bst1107I GTATAC 1 cut(s) 1190
Bst2BI CACGAG 1 cut(s) 2532
Bst2UI CCWGG 3 cut(s) 904, 2787, 3128
Bst6I CTCTTC 2 cut(s) 1860, 2064
BstACI GRCGYC 6 cut(s) 8, 182, 231, 282, 2783, 3120
BstAFI CTTAAG 1 cut(s) 950
BstAPI GCANNNNNTGC 4 cut(s) 399, 790, 1334, 3068
BstAUI TGTACA 2 cut(s) 2765, 3441
BstBAI YACGTR 2 cut(s) 1492, 3029
BstBI TTCGAA 1 cut(s) 1214
BstC8I GCNNGC 9 cut(s) 235, 655, 737, 896, 909, 946, 1524, 1667, 1671
BstDEI CTNAG 7 cut(s) 413, 805, 881, 935, 1865, 2697, 3115
BstDSI CCRYGG 1 cut(s) 669
BstENI CCTNNNNNAGG 1 cut(s) 436
BstFNI CGCG 2 cut(s) 489, 586
BstH2I RGCGCY 3 cut(s) 234, 1865, 2786
BstHHI GCGC 5 cut(s) 233, 489, 1864, 2684, 2785
BstMAI GTCTC 6 cut(s) 57, 228, 2551, 2700, 2824, 2957
BstNI CCWGG 3 cut(s) 904, 2787, 3128
BstNSI RCATGY 2 cut(s) 355, 3239
BstSCI CCNGG 4 cut(s) 902, 1582, 2785, 3126
BstSFI CTRYAG 3 cut(s) 1388, 1695, 1968
BstSNI TACGTA 1 cut(s) 1492
BstUI CGCG 2 cut(s) 489, 586
BstV2I GAAGAC 5 cut(s) 287, 753, 1404, 2097, 3318
BstX2I RGATCY 5 cut(s) 967, 1195, 1424, 2493, 2753
BstYI RGATCY 5 cut(s) 967, 1195, 1424, 2493, 2753
BstZ17I GTATAC 1 cut(s) 1190
BsuI GTATCC 1 cut(s) 1620
BsuRI GGCC 6 cut(s) 653, 907, 1372, 1665, 1775, 2447
BtgI CCRYGG 1 cut(s) 669
BtgZI GCGATG 1 cut(s) 510
BtsI GCAGTG 2 cut(s) 1238, 2243
BtsIMutI CAGTG 8 cut(s) 750, 1126, 1140, 1238, 1261, 2243, 3279, 3326
BveI ACCTGC 1 cut(s) 1381
Cac8I GCNNGC 9 cut(s) 235, 655, 737, 896, 909, 946, 1524, 1667, 1671
CaiI CAGNNNCTG 3 cut(s) 412, 3068, 3133
CciI TCATGA 1 cut(s) 3265
CfoI GCGC 5 cut(s) 233, 489, 1864, 2684, 2785
Cfr10I RCCGGY 4 cut(s) 25, 233, 653, 907
Cfr13I GGNCC 5 cut(s) 651, 1370, 2445, 2551, 2917
CpoI CGGWCCG 1 cut(s) 2551
CseI GACGC 1 cut(s) 2678
Csp6I GTAC 5 cut(s) 1262, 2405, 2417, 2766, 3442
CspI CGGWCCG 1 cut(s) 2551
CviQI GTAC 5 cut(s) 1262, 2405, 2417, 2766, 3442
DdeI CTNAG 7 cut(s) 413, 805, 881, 935, 1865, 2697, 3115
DinI GGCGCC 2 cut(s) 232, 2784
DraI TTTAAA 2 cut(s) 1648, 2080
DriI GACNNNNNGTC 1 cut(s) 835
Eam1104I CTCTTC 2 cut(s) 1860, 2064
Eam1105I GACNNNNNGTC 1 cut(s) 835
EarI CTCTTC 2 cut(s) 1860, 2064
EciI GGCGGA 3 cut(s) 55, 139, 470
Ecl136II GAGCTC 3 cut(s) 549, 1043, 2735
Eco105I TACGTA 1 cut(s) 1492
Eco130I CCWWGG 3 cut(s) 1271, 1450, 1770
Eco24I GRGCYC 4 cut(s) 551, 1045, 2737, 2995
Eco31I GGTCTC 1 cut(s) 2551
Eco32I GATATC 2 cut(s) 676, 1557
Eco47I GGWCC 2 cut(s) 2551, 2917
Eco53kI GAGCTC 3 cut(s) 549, 1043, 2735
Eco57I CTGAAG 7 cut(s) 579, 1727, 1841, 1980, 2109, 2406, 2655
Eco88I CYCGRG 1 cut(s) 1044
EcoICRI GAGCTC 3 cut(s) 549, 1043, 2735
EcoNI CCTNNNNNAGG 1 cut(s) 436
EcoRII CCWGG 3 cut(s) 902, 2785, 3126
EcoRV GATATC 2 cut(s) 676, 1557
EcoT14I CCWWGG 3 cut(s) 1271, 1450, 1770
EcoT38I GRGCYC 4 cut(s) 551, 1045, 2737, 2995
EgeI GGCGCC 2 cut(s) 232, 2784
EheI GGCGCC 2 cut(s) 232, 2784
ErhI CCWWGG 3 cut(s) 1271, 1450, 1770
Esp3I CGTCTC 1 cut(s) 57
FalI AAGNNNNNCTT 8 cut(s) 1411, 1443, 1725, 1757, 2628, 2660, 3270, 3302
FaqI GGGAC 4 cut(s) 112, 1034, 2384, 3367
FauI CCCGC 2 cut(s) 1842, 2317
FauNDI CATATG 3 cut(s) 2173, 2262, 2585
FbaI TGATCA 3 cut(s) 210, 1654, 3262
FblI GTMKAC 1 cut(s) 1189
FriOI GRGCYC 4 cut(s) 551, 1045, 2737, 2995
FspBI CTAG 6 cut(s) 648, 1272, 2180, 2199, 2738, 3341
GlaI GCGC 5 cut(s) 232, 488, 1863, 2683, 2784
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 1 cut(s) 3155
HaeII RGCGCY 3 cut(s) 234, 1865, 2786
HaeIII GGCC 6 cut(s) 653, 907, 1372, 1665, 1775, 2447
HapII CCGG 7 cut(s) 26, 234, 452, 654, 908, 1584, 3362
HgaI GACGC 1 cut(s) 2678
HhaI GCGC 5 cut(s) 233, 489, 1864, 2684, 2785
Hin1I GRCGYC 6 cut(s) 8, 182, 231, 282, 2783, 3120
Hin6I GCGC 5 cut(s) 231, 487, 1862, 2682, 2783
HinP1I GCGC 5 cut(s) 231, 487, 1862, 2682, 2783
HincII GTYRAC 2 cut(s) 3219, 3359
HindII GTYRAC 2 cut(s) 3219, 3359
HpaI GTTAAC 1 cut(s) 3219
HpaII CCGG 7 cut(s) 26, 234, 452, 654, 908, 1584, 3362
Hpy166II GTNNAC 6 cut(s) 324, 932, 1190, 2664, 3219, 3359
Hpy8I GTNNAC 6 cut(s) 324, 932, 1190, 2664, 3219, 3359
HpyAV CCTTC 9 cut(s) 262, 491, 1156, 1318, 1383, 2178, 2353, 2430, 2630
HpyCH4IV ACGT 6 cut(s) 182, 282, 1491, 1802, 3028, 3120
HpyF3I CTNAG 7 cut(s) 413, 805, 881, 935, 1865, 2697, 3115
HpySE526I ACGT 6 cut(s) 182, 282, 1491, 1802, 3028, 3120
Hsp92I GRCGYC 6 cut(s) 8, 182, 231, 282, 2783, 3120
HspAI GCGC 5 cut(s) 231, 487, 1862, 2682, 2783
KasI GGCGCC 2 cut(s) 230, 2782
KroI GCCGGC 3 cut(s) 233, 653, 907
KroNI GCCGGC 3 cut(s) 235, 655, 909
Ksp22I TGATCA 3 cut(s) 210, 1654, 3262
KspAI GTTAAC 1 cut(s) 3219
LguI GCTCTTC 1 cut(s) 1860
LmnI GCTCC 5 cut(s) 1040, 2125, 2170, 2998, 3136
LweI GCATC 7 cut(s) 1346, 1535, 2107, 2315, 3095, 3182, 3466
MaeI CTAG 6 cut(s) 648, 1272, 2180, 2199, 2738, 3341
MaeII ACGT 6 cut(s) 182, 282, 1491, 1802, 3028, 3120
MaeIII GTNAC 6 cut(s) 178, 1115, 2311, 2525, 2896, 3024
MfeI CAATTG 4 cut(s) 2250, 2657, 2769, 3465
MflI RGATCY 5 cut(s) 967, 1195, 1424, 2493, 2753
MhlI GDGCHC 6 cut(s) 431, 551, 1045, 2737, 2995, 3335
Mly113I GGCGCC 2 cut(s) 231, 2783
MlyI GAGTC 6 cut(s) 73, 319, 403, 793, 1418, 3356
MmeI TCCRAC 5 cut(s) 159, 544, 1344, 1984, 3063
MreI CGCCGGCG 1 cut(s) 233
MroNI GCCGGC 3 cut(s) 233, 653, 907
MroXI GAANNNNTTC 3 cut(s) 1149, 1740, 2058
MslI CAYNNNNRTG 2 cut(s) 755, 2144
MspA1I CMGCKG 5 cut(s) 469, 2033, 2435, 3065, 3439
MspCI CTTAAG 1 cut(s) 950
MspI CCGG 7 cut(s) 26, 234, 452, 654, 908, 1584, 3362
MspR9I CCNGG 4 cut(s) 904, 1584, 2787, 3128
MunI CAATTG 4 cut(s) 2250, 2657, 2769, 3465
Mva1269I GAATGC 5 cut(s) 404, 529, 620, 1149, 1584
MvaI CCWGG 3 cut(s) 904, 2787, 3128
MvnI CGCG 2 cut(s) 489, 586
NaeI GCCGGC 3 cut(s) 235, 655, 909
NarI GGCGCC 2 cut(s) 231, 2783
NciI CCSGG 1 cut(s) 1584
NdeI CATATG 3 cut(s) 2173, 2262, 2585
NgoMIV GCCGGC 3 cut(s) 233, 653, 907
NlaIV GGNNCC 3 cut(s) 232, 2784, 2994
NmeAIII GCCGAG 1 cut(s) 3496
NmuCI GTSAC 2 cut(s) 178, 1115
NspI RCATGY 2 cut(s) 355, 3239
NspV TTCGAA 1 cut(s) 1214
PaeR7I CTCGAG 1 cut(s) 1044
PagI TCATGA 1 cut(s) 3265
PciSI GCTCTTC 1 cut(s) 1860
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 5 cut(s) 404, 529, 620, 1149, 1584
PdiI GCCGGC 3 cut(s) 235, 655, 909
PdmI GAANNNNTTC 3 cut(s) 1149, 1740, 2058
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 3133
PleI GAGTC 6 cut(s) 73, 319, 403, 793, 1417, 3355
PluTI GGCGCC 2 cut(s) 234, 2786
PpsI GAGTC 6 cut(s) 73, 319, 403, 793, 1417, 3355
Ppu21I YACGTR 2 cut(s) 1492, 3029
PshBI ATTAAT 1 cut(s) 3431
Psp124BI GAGCTC 3 cut(s) 551, 1045, 2737
Psp6I CCWGG 3 cut(s) 902, 2785, 3126
PspFI CCCAGC 1 cut(s) 389
PspGI CCWGG 3 cut(s) 902, 2785, 3126
PspN4I GGNNCC 3 cut(s) 232, 2784, 2994
PspPI GGNCC 5 cut(s) 651, 1370, 2445, 2551, 2917
PspXI VCTCGAGB 1 cut(s) 1044
PstI CTGCAG 2 cut(s) 1392, 1972
PstNI CAGNNNCTG 3 cut(s) 412, 3068, 3133
PsuI RGATCY 5 cut(s) 967, 1195, 1424, 2493, 2753
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 5 cut(s) 469, 2033, 2435, 3065, 3439
RsaI GTAC 5 cut(s) 1263, 2406, 2418, 2767, 3443
RsaNI GTAC 5 cut(s) 1262, 2405, 2417, 2766, 3442
RseI CAYNNNNRTG 2 cut(s) 755, 2144
Rsr2I CGGWCCG 1 cut(s) 2551
RsrII CGGWCCG 1 cut(s) 2551
SacI GAGCTC 3 cut(s) 551, 1045, 2737
SapI GCTCTTC 1 cut(s) 1860
Sau96I GGNCC 5 cut(s) 651, 1370, 2445, 2551, 2917
SbfI CCTGCAGG 1 cut(s) 1972
SchI GAGTC 6 cut(s) 73, 319, 403, 793, 1418, 3356
ScrFI CCNGG 4 cut(s) 904, 1584, 2787, 3128
SdaI CCTGCAGG 1 cut(s) 1972
SduI GDGCHC 6 cut(s) 431, 551, 1045, 2737, 2995, 3335
SfaNI GCATC 7 cut(s) 1346, 1535, 2107, 2315, 3095, 3182, 3466
SfcI CTRYAG 3 cut(s) 1388, 1695, 1968
SfoI GGCGCC 2 cut(s) 232, 2784
Sfr274I CTCGAG 1 cut(s) 1044
SfuI TTCGAA 1 cut(s) 1214
SgrAI CRCCGGYG 2 cut(s) 25, 233
SinI GGWCC 2 cut(s) 2551, 2917
SlaI CTCGAG 1 cut(s) 1044
SmiMI CAYNNNNRTG 2 cut(s) 755, 2144
SmlI CTYRAG 6 cut(s) 950, 1044, 1053, 2472, 2864, 3386
SmoI CTYRAG 6 cut(s) 950, 1044, 1053, 2472, 2864, 3386
SnaBI TACGTA 1 cut(s) 1492
Sse8387I CCTGCAGG 1 cut(s) 1972
SsiI CCGC 9 cut(s) 40, 150, 153, 170, 455, 584, 1724, 1835, 2324
SspDI GGCGCC 2 cut(s) 230, 2782
SspMI CTAG 6 cut(s) 648, 1272, 2180, 2199, 2738, 3341
SstI GAGCTC 3 cut(s) 551, 1045, 2737
StyD4I CCNGG 4 cut(s) 902, 1582, 2785, 3126
StyI CCWWGG 3 cut(s) 1271, 1450, 1770
TaiI ACGT 6 cut(s) 185, 285, 1494, 1805, 3031, 3123
TaqI TCGA 8 cut(s) 533, 761, 1045, 1160, 1214, 1848, 2357, 3350
TaqII GACCGA 2 cut(s) 2539, 3414
TatI WGTACW 3 cut(s) 1261, 2765, 3441
TauI GCSGC 2 cut(s) 155, 586
TscAI CASTG 8 cut(s) 757, 1126, 1147, 1245, 1261, 2243, 3279, 3333
TseFI GTSAC 2 cut(s) 178, 1115
Tsp45I GTSAC 2 cut(s) 178, 1115
TspGWI ACGGA 2 cut(s) 218, 1990
TspRI CASTG 8 cut(s) 757, 1126, 1147, 1245, 1261, 2243, 3279, 3333
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 1 cut(s) 3133
Vha464I CTTAAG 1 cut(s) 950
VpaK11BI GGWCC 2 cut(s) 2551, 2917
VspI ATTAAT 1 cut(s) 3431
XagI CCTNNNNNAGG 1 cut(s) 436
XapI RAATTY 6 cut(s) 1917, 1996, 2649, 2774, 3184, 3476
XceI RCATGY 2 cut(s) 355, 3239
XhoI CTCGAG 1 cut(s) 1044
XmaJI CCTAGG 1 cut(s) 1271
XmiI GTMKAC 1 cut(s) 1189
XmnI GAANNNNTTC 3 cut(s) 1149, 1740, 2058
XspI CTAG 6 cut(s) 648, 1272, 2180, 2199, 2738, 3341
ZraI GACGTC 3 cut(s) 183, 283, 3121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.