MD14G1142900.v1.1

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003181
Physical Location & Seq
Forward (+)
23562383 .. 23570846
8464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1142900.v1.1.491

Sequence Viewer

Length: 798 bp
ATGTCCAGGCCTCCACACCTGCGCCAAGGGGACAATTCAAATATGAAGCAGAGTGAAGCTCGGGGTTCTCAAAGTTTATCAGATCATGAATCTTCCGCGAATGAGTTTGCATTGTCAGATTCAGATTACGGTGACAGTGATGGATCAATTAAAGACACTGACAACATTCAGAAATCAAAAGTCCGAGTAGCTGCCATTGTTTGTATACAGGATCTTTGTCAAGCTGATTCCAAATCATTTACTAGCCAATGGACGCTGCTTTTGCCAACCAGTGATGTACTGCGACCAAGGAAATATGAAGCAACACTAATGACATGCTTGCTATTTGATCCTTATCTAAAGGCACGGGTTGCTTCTGCATCAACCCTGGAAGCAATGTTGGATGGCCCATCTTCTGTGTTTCTCCAGGTAGCAGAGTTCAAGGAATCTTCTAAACGTGGATCTTTCAAAGCGCTTTCAAGTTCCCTTGGACATATACTAATGCAGCTTCATACAGGTATTTTATACTTAATTCAATGTGAAACTCATAGTAGATTGATGGCATCCCTGCTCAAGATTCTCATGCTTTTGAGATCATCTACACCAATGCCTGGAGAGTTGATGCCAACAGTTTTTACATCTCTACAAGAAAGGATGAAAAATGGGTTTACATTCAAAAGTGGTCACACTGGTCTGCTGGCTTCTTCTATTAGTTGCTTGACAACTGCTCTGAATATCTCACCTTCTTCACTGCAAGTTAAAGAAATGGTTCTGGTAGAGATATCGAATGGTAGGCTTAATTTTGGTAGCATAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4042 HEAT

Protein Analysis

266

Amino Acids

29.01

Weight (kDa)

7.67

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4042 PF13251 60 - 237 9.3e-44 Domain of unknown function (DUF4042)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 27
Acc36I ACCTGC 1 cut(s) 27
AccB7I CCANNNNNTGG 1 cut(s) 590
AccI GTMKAC 1 cut(s) 205
AccII CGCG 1 cut(s) 98
AciI CCGC 1 cut(s) 96
AclWI GGATC 4 cut(s) 151, 219, 323, 448
AfaI GTAC 1 cut(s) 279
AfeI AGCGCT 1 cut(s) 453
AfiI CCNNNNNNNGG 1 cut(s) 590
AgsI TTSAA 6 cut(s) 39, 421, 448, 459, 515, 655
AjnI CCWGG 4 cut(s) 5, 366, 405, 589
AluBI AGCT 4 cut(s) 59, 191, 224, 487
AluI AGCT 4 cut(s) 59, 191, 224, 487
AlwI GGATC 4 cut(s) 151, 219, 323, 448
Ama87I CYCGRG 1 cut(s) 60
Aor51HI AGCGCT 1 cut(s) 453
AoxI GGCC 2 cut(s) 8, 385
ApeKI GCWGC 3 cut(s) 191, 256, 484
ArsI GACNNNNNNTTYG 2 cut(s) 244, 276
Asp700I GAANNNNTTC 1 cut(s) 747
AspLEI GCGC 2 cut(s) 24, 454
AspS9I GGNCC 1 cut(s) 386
AsuHPI GGTGA 2 cut(s) 143, 711
AvaI CYCGRG 1 cut(s) 60
BbvI GCAGC 3 cut(s) 178, 243, 496
BccI CCATC 4 cut(s) 134, 377, 397, 532
BciT130I CCWGG 4 cut(s) 7, 368, 407, 591
BfaI CTAG 1 cut(s) 243
BfoI RGCGCY 1 cut(s) 455
BfuAI ACCTGC 1 cut(s) 27
BisI GCNGC 3 cut(s) 192, 257, 485
BlsI GCNGC 3 cut(s) 193, 258, 486
Bme1390I CCNGG 4 cut(s) 7, 368, 407, 591
BmeT110I CYCGRG 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 386
BmrFI CCNGG 4 cut(s) 7, 368, 407, 591
BmsI GCATC 3 cut(s) 368, 551, 591
BplI GAGNNNNNCTC 2 cut(s) 43, 75
BpmI CTGGAG 2 cut(s) 389, 612
BpuEI CTTGAG 1 cut(s) 536
BsaBI GATNNNNATC 1 cut(s) 333
BsaJI CCNNGG 4 cut(s) 25, 287, 366, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse1I ACTGG 2 cut(s) 270, 673
Bse3DI GCAATG 1 cut(s) 381
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 4 cut(s) 7, 368, 407, 591
BseDI CCNNGG 4 cut(s) 25, 287, 366, 466
BseGI GGATG 3 cut(s) 388, 542, 639
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMI GCAATG 1 cut(s) 381
BseNI ACTGG 2 cut(s) 270, 673
BseXI GCAGC 3 cut(s) 178, 243, 496
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 2 cut(s) 10, 387
BsiHKCI CYCGRG 1 cut(s) 60
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 590
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 2 cut(s) 10, 387
BsoBI CYCGRG 1 cut(s) 60
Bsp143I GATC 6 cut(s) 82, 143, 211, 328, 440, 572
BspACI CCGC 1 cut(s) 96
BspANI GGCC 2 cut(s) 10, 387
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 27
BspPI GGATC 4 cut(s) 151, 219, 323, 448
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 2 cut(s) 270, 673
BssECI CCNNGG 4 cut(s) 25, 287, 366, 466
BssMI GATC 6 cut(s) 82, 143, 211, 328, 440, 572
BssNAI GTATAC 1 cut(s) 206
BssT1I CCWWGG 3 cut(s) 25, 287, 466
Bst1107I GTATAC 1 cut(s) 206
Bst2UI CCWGG 4 cut(s) 7, 368, 407, 591
Bst4CI ACNGT 3 cut(s) 131, 137, 610
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 320, 678
BstF5I GGATG 3 cut(s) 388, 542, 639
BstFNI CGCG 1 cut(s) 98
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 2 cut(s) 24, 454
BstKTI GATC 6 cut(s) 85, 146, 214, 331, 443, 575
BstMBI GATC 6 cut(s) 82, 143, 211, 328, 440, 572
BstMWI GCNNNNNNNGC 2 cut(s) 262, 350
BstNI CCWGG 4 cut(s) 7, 368, 407, 591
BstNSI RCATGY 1 cut(s) 318
BstSCI CCNGG 4 cut(s) 5, 366, 405, 589
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 3 cut(s) 178, 243, 496
BstX2I RGATCY 2 cut(s) 211, 440
BstYI RGATCY 2 cut(s) 211, 440
BstZ17I GTATAC 1 cut(s) 206
BsuRI GGCC 2 cut(s) 10, 387
BtsCI GGATG 3 cut(s) 388, 542, 639
BtsI GCAGTG 1 cut(s) 728
BtsIMutI CAGTG 5 cut(s) 142, 156, 277, 666, 728
BveI ACCTGC 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 320, 678
CciI TCATGA 1 cut(s) 85
CfoI GCGC 2 cut(s) 24, 454
Cfr13I GGNCC 1 cut(s) 386
CseI GACGC 1 cut(s) 262
Csp6I GTAC 1 cut(s) 278
CviAII CATG 3 cut(s) 86, 315, 562
CviJI RGCY 9 cut(s) 10, 59, 191, 224, 246, 387, 487, 680, 775
CviKI_1 RGCY 9 cut(s) 10, 59, 191, 224, 246, 387, 487, 680, 775
CviQI GTAC 1 cut(s) 278
DpnI GATC 6 cut(s) 84, 145, 213, 330, 442, 574
DpnII GATC 6 cut(s) 82, 143, 211, 328, 440, 572
Eco130I CCWWGG 3 cut(s) 25, 287, 466
Eco147I AGGCCT 1 cut(s) 10
Eco32I GATATC 1 cut(s) 762
Eco47III AGCGCT 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 60
EcoRII CCWGG 4 cut(s) 5, 366, 405, 589
EcoRV GATATC 1 cut(s) 762
EcoT14I CCWWGG 3 cut(s) 25, 287, 466
ErhI CCWWGG 3 cut(s) 25, 287, 466
FaeI CATG 3 cut(s) 89, 318, 565
FaqI GGGAC 1 cut(s) 44
FatI CATG 3 cut(s) 85, 314, 561
FblI GTMKAC 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 192, 257, 485
FokI GGATG 3 cut(s) 395, 529, 646
Fsp4HI GCNGC 3 cut(s) 192, 257, 485
FspBI CTAG 1 cut(s) 243
GlaI GCGC 2 cut(s) 23, 453
GluI GCNGC 3 cut(s) 192, 257, 485
GsuI CTGGAG 2 cut(s) 389, 612
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 2 cut(s) 10, 387
HgaI GACGC 1 cut(s) 262
HhaI GCGC 2 cut(s) 24, 454
Hin1II CATG 3 cut(s) 89, 318, 565
Hin6I GCGC 2 cut(s) 22, 452
HinP1I GCGC 2 cut(s) 22, 452
HinfI GANTC 5 cut(s) 89, 119, 227, 425, 556
HphI GGTGA 2 cut(s) 143, 711
Hpy166II GTNNAC 2 cut(s) 206, 648
Hpy188I TCNGA 6 cut(s) 82, 118, 124, 171, 185, 711
Hpy188III TCNNGA 2 cut(s) 86, 553
Hpy8I GTNNAC 2 cut(s) 206, 648
HpyAV CCTTC 1 cut(s) 732
HpyCH4III ACNGT 3 cut(s) 131, 137, 610
HpyCH4IV ACGT 1 cut(s) 436
HpyCH4V TGCA 4 cut(s) 110, 359, 484, 733
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 350
HpySE526I ACGT 1 cut(s) 436
Hsp92II CATG 3 cut(s) 89, 318, 565
HspAI GCGC 2 cut(s) 22, 452
Kzo9I GATC 6 cut(s) 82, 143, 211, 328, 440, 572
Lsp1109I GCAGC 3 cut(s) 178, 243, 496
LweI GCATC 3 cut(s) 368, 551, 591
MaeI CTAG 1 cut(s) 243
MaeII ACGT 1 cut(s) 436
MaeIII GTNAC 2 cut(s) 131, 662
MalI GATC 6 cut(s) 84, 145, 213, 330, 442, 574
MboI GATC 6 cut(s) 82, 143, 211, 328, 440, 572
MboII GAAGA 5 cut(s) 84, 384, 420, 675, 717
MflI RGATCY 2 cut(s) 211, 440
MluCI AATT 4 cut(s) 34, 147, 510, 778
MmeI TCCRAC 1 cut(s) 360
MnlI CCTC 1 cut(s) 21
MroXI GAANNNNTTC 1 cut(s) 747
MseI TTAA 4 cut(s) 150, 509, 738, 777
MspR9I CCNGG 4 cut(s) 7, 368, 407, 591
MvaI CCWGG 4 cut(s) 7, 368, 407, 591
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 2 cut(s) 262, 350
NdeII GATC 6 cut(s) 82, 143, 211, 328, 440, 572
NlaIII CATG 3 cut(s) 89, 318, 565
NmuCI GTSAC 2 cut(s) 131, 662
NspI RCATGY 1 cut(s) 318
PagI TCATGA 1 cut(s) 85
PaqCI CACCTGC 1 cut(s) 27
PceI AGGCCT 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 747
PfeI GAWTC 5 cut(s) 89, 119, 227, 425, 556
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 3 cut(s) 193, 258, 486
Psp6I CCWGG 4 cut(s) 5, 366, 405, 589
PspGI CCWGG 4 cut(s) 5, 366, 405, 589
PspPI GGNCC 1 cut(s) 386
PsuI RGATCY 2 cut(s) 211, 440
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SaqAI TTAA 4 cut(s) 150, 509, 738, 777
SatI GCNGC 3 cut(s) 192, 257, 485
Sau3AI GATC 6 cut(s) 82, 143, 211, 328, 440, 572
Sau96I GGNCC 1 cut(s) 386
ScrFI CCNGG 4 cut(s) 7, 368, 407, 591
SetI ASST 9 cut(s) 21, 61, 193, 226, 411, 439, 489, 499, 724
SfaNI GCATC 3 cut(s) 368, 551, 591
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 4 cut(s) 34, 147, 510, 778
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 1 cut(s) 96
SspMI CTAG 1 cut(s) 243
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 4 cut(s) 5, 366, 405, 589
StyI CCWWGG 3 cut(s) 25, 287, 466
TaaI ACNGT 3 cut(s) 131, 137, 610
TaiI ACGT 1 cut(s) 439
TaqI TCGA 1 cut(s) 764
TasI AATT 4 cut(s) 34, 147, 510, 778
TatI WGTACW 1 cut(s) 277
TfiI GAWTC 5 cut(s) 89, 119, 227, 425, 556
Tru1I TTAA 4 cut(s) 150, 509, 738, 777
Tru9I TTAA 4 cut(s) 150, 509, 738, 777
TscAI CASTG 5 cut(s) 142, 163, 277, 673, 735
TseFI GTSAC 2 cut(s) 131, 662
TseI GCWGC 3 cut(s) 191, 256, 484
Tsp45I GTSAC 2 cut(s) 131, 662
TspDTI ATGAA 5 cut(s) 59, 102, 312, 479, 650
TspRI CASTG 5 cut(s) 142, 163, 277, 673, 735
Van91I CCANNNNNTGG 1 cut(s) 590
XceI RCATGY 1 cut(s) 318
XmiI GTMKAC 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 747
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.