Rh7AG092600

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0003181
Physical Location & Seq
Forward (+)
7058523 .. 7066881
8359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG092600.1

Sequence Viewer

Length: 3540 bp
ATGGGAGGGACTGAGAAGACGAGCAAGGTCGTTATGACGGCGTCTGTATCACAATCGCCGGTCAGGTGGTGGCGGACGGCGTTTCTGACCGTCAGAGACGAAACCCTCACGACTCCTCCTCGCACTCCAATCCCCGAACTCCTCCACAATTTCATCTTCTCCCACTCCCACACTCTCCTCTCCGCCGCCCCCGACCTCCCTCCGCAGGAGGTGACGTCAGATTTGTTGTTCCTAACGGAATTGATCACCACCAAGTCTCACGGCGCCGGCGATTTGACCCCTACCTTCGCTCACACAATTCACCTGATCCATGACGTTAGCCATCGTCTTCCTCTGGAAGTCAATTCAGCTTCGTGGACTCTCATGCTTGACGGTTTCGGCAACATGCTTCAATTCTTCATTGCCTCTTCGTCATTTACGCCCAGCATGGAATGCTTACAGACTCTGAGGCGTGTTATGAGCACTTACCAGAGGAAGTGTTCTCCGGCGGATGAGATTCAGCTGGTTAAGTTCCTTCTGCGCGTAATTGAGAGCTGTCATTCAGAGTTAAGTAGCTCATTGCATTCAATTCGCAACCAGAGCTCTGCTTCTGAAGTTGGAAAGAGAAAGCCATTGCCGCAACATTGCAGCTTATGGGAAGCTCAGACCCTTGCATTCAATATGCTTCGAGAAACAATCTCTAGGGCCGGCTCGTTGTTTCCCGTGGATATCTGGAAATCATCTATTGAGGTTTTTAGGAAAGTGATGGATGTATTGGCAGCTAAAAGCCTGCTTGTAGAAGACACTGTACTGTCGAGGTTTTATTTATCTCTCCTGCATTGTCTGCACTTGACTCTCTCAGATCGTAAGTGTTCCCACTCTGACCATGTGTCAGGTTTTGTAGCTGCTTTGCGAATGTTCTTGTCCTATGGTATTAGTAGTAGAAGCCAGCTTACCAGGCCGGCAACTGGTCAAAAGGAGAGTGAACTTAGTGTAGCCAGCCTTAAGTCTGGTTTGGAAGATCCTAAAAAGACAGATCGTAGTCCATATAGACCTCCACACCTACGCAAAAGGGACAGTTCAAATCAGATTGGAGCTCGAGGTTCTCAAGGTTTATCAGATCAGGAATCTTCCACACTTGACTTTGCCTCATCAGATTCAGATTATAGTGACAGTGATGGATCACTTAAAGACACTGAAAGCATTCAGAAGTCGAAGGTCAGAGTAGCTGCCATTGTTTGTATACAGGATCTCTGTCAAGCTGATTCGAAATCATTTAGTAGCCAATGGACACTGCTTTTGCCAACCAGTGATGTACTACAACCTAGGAAGTTTGAAGCAACACTAATGACTTGTTTGCTGTTTGATCCCTATTTGAAGGCACGGGTTGCATCTGCCTCAACTCTGGAAGCAATGTTGGATGGGCCTTCTTCTGTTTTTCTGCAGGTAGCAGAGTTTAAAGAGTCTTCTAAAAGGGGATCTTTTACAGCACTTTCAAGTTCCCTTGGACATATACTAATGCAGCTTCATACAGGTATTTTATACTTAATTCAACGAGAAACTCATAGTAGATTGCTGGCATCCTTGTTCAAGATTCTCATGCTTTTGATATCATCTACACCATATTCAAGAATGCCTGGAGAATTGTTGCCAACAGTTTTTACATCTCTACAAGAAAGGATACAAAATGGGTTTCAGTTTAAAAGTGATCAGACTGGCCTGCTGGTTTCTTCTTTTAGTTGCTTGACTACAGCTCTGAATACCTCACCATCCTCACCGCAAATCAAAGAAATGCTTCAGAGAGAGATATTTAATGGTTTTGCCGAGGCCAAAAAGAAGTCGGGTGTTCTTTTTACGTTGTTTCAATTTTCAGAACAAGTTAGCAACCCGCCAATATGCTTCGAGGCTCTTCAGGCGCTTAGGGCTGTGTCGCACAATTACCCCAGTATAATGTTTTCCTGTTGGGAACAAATTTCCACCATTGTTTATCACCTTTTGAGGGCAGCTACTCCTGAAGTTCCTGCAGGACAATGGAAAGGGCATACTGGAAATTCTGTTGGATTCATTGGGGAAAAACTCATTACAGCTGCTATTAGAGTTTTGGATGAATCTCTTCGTGCAATATCTGGATTTAAAGGAACGGAAGACCCTTTAGACGATAAACTACTTGATGCCCCATTTACTTCTGACTGCATAAGGATGAAGAAAGTTTCATCAGCTCCTTCATATGAACTAGAGAGCTTGGAAAATACTAGAGATGAACCCACATCATGTCAGTCTGGAATTGAGCAGTGGTGTGAGGCAATTGAGAAGCATATGCCTTTAATTCTACAACATACTTCTGCAATGGTGAGAGCTGCCTCAGTTACCTGTTTTGCGGGTATAACTTCTTCTGTGTTCTGCACACTCTCGAAGGAAAAACGGGACTTCATTCTTTCATCTTTAGTTCGTGCTGCCATTCACGGTGATGTACCTTCAGTTAGGGCAGCTGCTTGTCGGGCCATTGGTGTCATTTCATGTTTTCCTCAAGTTTCTCAAAGTGCAGAGATCCTTGATAAGTTTGTTCATGCTGTGGAGAGTAACACTCGTGATCCTTTGGTCTCGGTCCGAATAACTGCTTCTTGGGCTTTGGCAAACATATGTGATTCTATCCATCATTGTATTGATGATTTTAGCTTGGAAAAGACTGAAGGCTCTCTTAAAATTTCTCAATTATTTACCTTATTAACCGAGTGCGCTTTGCGTCTGACTAAGGACGGAGACAAGATTAAATCAAATGCTGTAAGAGCTCTAGGGAATCTTTCAAGATCTATCAAATGTACAATTGAATTTGAGGCGCCAGGGGATTATGGGAAAGGTTGTCAAAGAGATGTATCAATATCATACTATCCAGCTTCTTTGAGAGATTCGCGTTGGCTTGATAGGGTTGTTCAGGCATTTATTTCTTGTGTTACGACTGGTAATGTCAAGGTCCAATGGAATGTTTGTCATGCTTTGAGCAACCTATTTCTGAACGAGACATTAAGACTGCAAGACATGGATTGGGCTCCATCTGTGTTTAGTATTCTTCTACTACTGTTACGTGATTCTTCCAACTTTAAGATCAGAATCCAAGCAGCTGCTGCTCTGGCTGTTCCAGCATCGGTGCATGATTATGGGGAATCTTTCTCAGACGTCATCCAGGGTCTGGAGCATATATTAGAGAATCAAGGTTCAGACCAGATTGCATCACCATCAAATTTCAAATATAGGGTTGCCCTTGAAAAGCAGTTAACCTCAACCATGTTGCATGTTCTCATCCTCGCTTCAAGCTCTGATCATGAACCAGTGAAAGTTTTTCTTGTAAAAAAAGCCTCATTCTTTGAAGACTGGTTCAAGGCACTGTGCTCATCACTAGGAGAGTCGAGTAGTCAACCGGAACTTGAAAATAAGAAGTCCCTTGAGAACCCAAAGAAAGAGATGATATGCAACGCAATTCGGTCACTAATTCAGCTGTACAATGACAGGAAGCATCATGCAATTGCCGAGAAATTTGAGAAGTTAGAGAAAAGCATACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4042 HEAT

Protein Analysis

1179

Amino Acids

130.8

Weight (kDa)

7.06

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4042 PF13251 399 - 582 2.4e-52 Domain of unknown function (DUF4042)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 218, 3156
Acc36I ACCTGC 1 cut(s) 1414
AccB1I GGYRCC 2 cut(s) 263, 2815
AccB7I CCANNNNNTGG 1 cut(s) 3166
AccI GTMKAC 1 cut(s) 1222
AccII CGCG 2 cut(s) 522, 2890
AciI CCGC 9 cut(s) 73, 183, 186, 203, 488, 617, 1757, 1868, 2357
AclWI GGATC 8 cut(s) 301, 995, 1168, 1236, 1340, 1465, 2521, 2564
AcsI RAATTY 6 cut(s) 1950, 2029, 2682, 2807, 3217, 3509
AcuI CTGAAG 6 cut(s) 612, 1760, 1874, 2013, 2439, 2688
AcyI GRCGYC 5 cut(s) 41, 215, 264, 2816, 3153
AfaI GTAC 5 cut(s) 789, 1296, 2451, 2800, 3476
AfiI CCNNNNNNNGG 4 cut(s) 205, 947, 1978, 3166
AflII CTTAAG 1 cut(s) 983
AhdI GACNNNNNGTC 1 cut(s) 868
AjnI CCWGG 4 cut(s) 935, 1615, 2818, 3159
Alw21I GWGCWC 5 cut(s) 464, 584, 1078, 2770, 3368
Alw26I GTCTC 5 cut(s) 90, 261, 2584, 2733, 2990
AlwI GGATC 8 cut(s) 301, 995, 1168, 1236, 1340, 1465, 2521, 2564
AlwNI CAGNNNCTG 3 cut(s) 445, 3101, 3166
Ama87I CYCGRG 1 cut(s) 1077
AoxI GGCC 6 cut(s) 684, 938, 1403, 1696, 1806, 2478
ApoI RAATTY 6 cut(s) 1950, 2029, 2682, 2807, 3217, 3509
ArsI GACNNNNNNTTYG 4 cut(s) 205, 237, 1261, 1293
Asp700I GAANNNNTTC 3 cut(s) 1182, 1773, 2091
AspA2I CCTAGG 1 cut(s) 1304
AspLEI GCGC 5 cut(s) 266, 522, 1897, 2717, 2818
AspS9I GGNCC 5 cut(s) 684, 1403, 2478, 2584, 2950
AsuHPI GGTGA 9 cut(s) 223, 238, 293, 1737, 1746, 1961, 2341, 2456, 3201
AsuII TTCGAA 1 cut(s) 1247
AvaI CYCGRG 1 cut(s) 1077
AvaII GGWCC 2 cut(s) 2584, 2950
AvrII CCTAGG 1 cut(s) 1304
BanI GGYRCC 2 cut(s) 263, 2815
BanII GRGCYC 4 cut(s) 584, 1078, 2770, 3028
BarI GAAGNNNNNNTAC 6 cut(s) 771, 803, 916, 948, 2353, 2385
BauI CACGAG 1 cut(s) 2565
BbsI GAAGAC 6 cut(s) 23, 320, 786, 1437, 2130, 3351
Bbv12I GWGCWC 5 cut(s) 464, 584, 1078, 2770, 3368
BccI CCATC 8 cut(s) 330, 739, 1151, 1394, 1756, 2640, 3037, 3220
BceAI ACGGC 3 cut(s) 54, 93, 277
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 4 cut(s) 937, 1617, 2820, 3161
BciVI GTATCC 1 cut(s) 1653
BclI TGATCA 3 cut(s) 243, 1687, 3295
BcoDI GTCTC 5 cut(s) 90, 261, 2584, 2733, 2990
BfaI CTAG 6 cut(s) 681, 1305, 2213, 2232, 2771, 3374
BfmI CTRYAG 3 cut(s) 1421, 1728, 2001
BfoI RGCGCY 3 cut(s) 267, 1898, 2819
BfrI CTTAAG 1 cut(s) 983
BfuAI ACCTGC 1 cut(s) 1414
BfuI GTATCC 1 cut(s) 1653
BglII AGATCT 1 cut(s) 2786
BlnI CCTAGG 1 cut(s) 1304
Bme1390I CCNGG 4 cut(s) 937, 1617, 2820, 3161
Bme18I GGWCC 2 cut(s) 2584, 2950
BmeRI GACNNNNNGTC 1 cut(s) 868
BmeT110I CYCGRG 1 cut(s) 1077
BmgT120I GGNCC 5 cut(s) 684, 1403, 2478, 2584, 2950
BmiI GGNNCC 3 cut(s) 265, 2817, 3027
BmrFI CCNGG 4 cut(s) 937, 1617, 2820, 3161
BmrI ACTGGG 1 cut(s) 1917
BmsI GCATC 6 cut(s) 1379, 1568, 2140, 3128, 3215, 3499
BmuI ACTGGG 1 cut(s) 1917
BpiI GAAGAC 6 cut(s) 23, 320, 786, 1437, 2130, 3351
BplI GAGNNNNNCTC 2 cut(s) 2548, 2580
BpmI CTGGAG 2 cut(s) 1638, 3188
Bpu10I CCTNAGC 1 cut(s) 1898
Bpu14I TTCGAA 1 cut(s) 1247
BpuEI CTTGAG 3 cut(s) 1071, 2490, 3440
BsaAI YACGTR 1 cut(s) 3062
BsaBI GATNNNNATC 1 cut(s) 3086
BsaHI GRCGYC 5 cut(s) 41, 215, 264, 2816, 3153
BsaI GGTCTC 1 cut(s) 2584
BsaJI CCNNGG 6 cut(s) 702, 1304, 1483, 1803, 2819, 3160
BsaWI WCCGGW 1 cut(s) 3394
BsaXI ACNNNNNCTCC 4 cut(s) 100, 130, 200, 230
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 947, 1978, 3166
Bse118I RCCGGY 4 cut(s) 58, 266, 686, 940
Bse1I ACTGG 8 cut(s) 952, 1287, 1699, 1923, 2029, 2941, 3305, 3353
Bse3DI GCAATG 6 cut(s) 399, 557, 611, 622, 1398, 2331
Bse8I GATNNNNATC 1 cut(s) 3086
BseBI CCWGG 4 cut(s) 937, 1617, 2820, 3161
BseDI CCNNGG 6 cut(s) 702, 1304, 1483, 1803, 2819, 3160
BseGI GGATG 9 cut(s) 496, 754, 1405, 1559, 1748, 2089, 2184, 3156, 3276
BseJI GATNNNNATC 1 cut(s) 3086
BseLI CCNNNNNNNGG 4 cut(s) 205, 947, 1978, 3166
BseMI GCAATG 6 cut(s) 399, 557, 611, 622, 1398, 2331
BseMII CTCAG 5 cut(s) 437, 656, 852, 2355, 3162
BseNI ACTGG 8 cut(s) 952, 1287, 1699, 1923, 2029, 2941, 3305, 3353
BseRI GAGGAG 4 cut(s) 105, 108, 131, 167
BseYI CCCAGC 1 cut(s) 422
BsgI GTGCAG 3 cut(s) 809, 2365, 2541
Bsh1236I CGCG 2 cut(s) 522, 2890
BshFI GGCC 6 cut(s) 686, 940, 1405, 1698, 1808, 2480
BshNI GGYRCC 2 cut(s) 263, 2815
BsiHKAI GWGCWC 5 cut(s) 464, 584, 1078, 2770, 3368
BsiHKCI CYCGRG 1 cut(s) 1077
BsiSI CCGG 6 cut(s) 59, 267, 485, 687, 941, 3395
BslFI GGGAC 4 cut(s) 22, 1067, 2417, 3400
BslI CCNNNNNNNGG 4 cut(s) 205, 947, 1978, 3166
BsmAI GTCTC 5 cut(s) 90, 261, 2584, 2733, 2990
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 4 cut(s) 22, 1067, 2417, 3400
BsmI GAATGC 5 cut(s) 437, 562, 653, 1182, 1617
BsnI GGCC 6 cut(s) 686, 940, 1405, 1698, 1808, 2480
Bso31I GGTCTC 1 cut(s) 2584
BsoBI CYCGRG 1 cut(s) 1077
Bsp119I TTCGAA 1 cut(s) 1247
Bsp1286I GDGCHC 6 cut(s) 464, 584, 1078, 2770, 3028, 3368
Bsp1407I TGTACA 2 cut(s) 2798, 3474
BspACI CCGC 9 cut(s) 73, 183, 186, 203, 488, 617, 1757, 1868, 2357
BspANI GGCC 6 cut(s) 686, 940, 1405, 1698, 1808, 2480
BspCNI CTCAG 6 cut(s) 4, 438, 655, 851, 2354, 3161
BspFNI CGCG 2 cut(s) 522, 2890
BspHI TCATGA 1 cut(s) 3298
BspLI GGNNCC 3 cut(s) 265, 2817, 3027
BspMAI CTGCAG 2 cut(s) 1425, 2005
BspMI ACCTGC 1 cut(s) 1414
BspPI GGATC 8 cut(s) 301, 995, 1168, 1236, 1340, 1465, 2521, 2564
BspQI GCTCTTC 1 cut(s) 1893
BspT104I TTCGAA 1 cut(s) 1247
BspT107I GGYRCC 2 cut(s) 263, 2815
BspTI CTTAAG 1 cut(s) 983
BspTNI GGTCTC 1 cut(s) 2584
BsrDI GCAATG 6 cut(s) 399, 557, 611, 622, 1398, 2331
BsrFI RCCGGY 4 cut(s) 58, 266, 686, 940
BsrGI TGTACA 2 cut(s) 2798, 3474
BsrI ACTGG 8 cut(s) 952, 1287, 1699, 1923, 2029, 2941, 3305, 3353
BssAI RCCGGY 4 cut(s) 58, 266, 686, 940
BssECI CCNNGG 6 cut(s) 702, 1304, 1483, 1803, 2819, 3160
BssNAI GTATAC 1 cut(s) 1223
BssNI GRCGYC 5 cut(s) 41, 215, 264, 2816, 3153
BssSI CACGAG 1 cut(s) 2565
BssT1I CCWWGG 2 cut(s) 1304, 1483
Bst1107I GTATAC 1 cut(s) 1223
Bst2BI CACGAG 1 cut(s) 2565
Bst2UI CCWGG 4 cut(s) 937, 1617, 2820, 3161
Bst6I CTCTTC 3 cut(s) 412, 1893, 2097
BstACI GRCGYC 5 cut(s) 41, 215, 264, 2816, 3153
BstAFI CTTAAG 1 cut(s) 983
BstAPI GCANNNNNTGC 4 cut(s) 432, 823, 1367, 3101
BstAUI TGTACA 2 cut(s) 2798, 3474
BstBAI YACGTR 1 cut(s) 3062
BstBI TTCGAA 1 cut(s) 1247
BstC8I GCNNGC 8 cut(s) 268, 688, 770, 929, 942, 979, 1557, 1700
BstDEI CTNAG 9 cut(s) 12, 446, 642, 838, 968, 1898, 2341, 2730, 3148
BstDSI CCRYGG 1 cut(s) 702
BstF5I GGATG 9 cut(s) 496, 754, 1405, 1559, 1748, 2089, 2184, 3156, 3276
BstFNI CGCG 2 cut(s) 522, 2890
BstH2I RGCGCY 3 cut(s) 267, 1898, 2819
BstHHI GCGC 5 cut(s) 266, 522, 1897, 2717, 2818
BstMAI GTCTC 5 cut(s) 90, 261, 2584, 2733, 2990
BstNI CCWGG 4 cut(s) 937, 1617, 2820, 3161
BstNSI RCATGY 2 cut(s) 388, 3272
BstSCI CCNGG 4 cut(s) 935, 1615, 2818, 3159
BstSFI CTRYAG 3 cut(s) 1421, 1728, 2001
BstUI CGCG 2 cut(s) 522, 2890
BstV2I GAAGAC 6 cut(s) 23, 320, 786, 1437, 2130, 3351
BstX2I RGATCY 5 cut(s) 1000, 1228, 1457, 2526, 2786
BstYI RGATCY 5 cut(s) 1000, 1228, 1457, 2526, 2786
BstZ17I GTATAC 1 cut(s) 1223
BsuI GTATCC 1 cut(s) 1653
BsuRI GGCC 6 cut(s) 686, 940, 1405, 1698, 1808, 2480
BtgI CCRYGG 1 cut(s) 702
BtsCI GGATG 9 cut(s) 496, 754, 1405, 1559, 1748, 2089, 2184, 3156, 3276
BtsI GCAGTG 2 cut(s) 1271, 2276
BtsIMutI CAGTG 8 cut(s) 783, 1159, 1173, 1271, 1294, 2276, 3312, 3359
BveI ACCTGC 1 cut(s) 1414
Cac8I GCNNGC 8 cut(s) 268, 688, 770, 929, 942, 979, 1557, 1700
CaiI CAGNNNCTG 3 cut(s) 445, 3101, 3166
CciI TCATGA 1 cut(s) 3298
CfoI GCGC 5 cut(s) 266, 522, 1897, 2717, 2818
Cfr10I RCCGGY 4 cut(s) 58, 266, 686, 940
Cfr13I GGNCC 5 cut(s) 684, 1403, 2478, 2584, 2950
CpoI CGGWCCG 1 cut(s) 2584
CseI GACGC 2 cut(s) 30, 2711
Csp6I GTAC 5 cut(s) 788, 1295, 2450, 2799, 3475
CspI CGGWCCG 1 cut(s) 2584
CviQI GTAC 5 cut(s) 788, 1295, 2450, 2799, 3475
DdeI CTNAG 9 cut(s) 12, 446, 642, 838, 968, 1898, 2341, 2730, 3148
DinI GGCGCC 2 cut(s) 265, 2817
DraI TTTAAA 3 cut(s) 1438, 1681, 2113
DriI GACNNNNNGTC 1 cut(s) 868
Eam1104I CTCTTC 3 cut(s) 412, 1893, 2097
Eam1105I GACNNNNNGTC 1 cut(s) 868
EarI CTCTTC 3 cut(s) 412, 1893, 2097
EciI GGCGGA 3 cut(s) 88, 172, 503
Ecl136II GAGCTC 3 cut(s) 582, 1076, 2768
Eco130I CCWWGG 2 cut(s) 1304, 1483
Eco24I GRGCYC 4 cut(s) 584, 1078, 2770, 3028
Eco31I GGTCTC 1 cut(s) 2584
Eco32I GATATC 2 cut(s) 709, 1590
Eco47I GGWCC 2 cut(s) 2584, 2950
Eco53kI GAGCTC 3 cut(s) 582, 1076, 2768
Eco57I CTGAAG 6 cut(s) 612, 1760, 1874, 2013, 2439, 2688
Eco88I CYCGRG 1 cut(s) 1077
EcoICRI GAGCTC 3 cut(s) 582, 1076, 2768
EcoRII CCWGG 4 cut(s) 935, 1615, 2818, 3159
EcoRV GATATC 2 cut(s) 709, 1590
EcoT14I CCWWGG 2 cut(s) 1304, 1483
EcoT38I GRGCYC 4 cut(s) 584, 1078, 2770, 3028
EgeI GGCGCC 2 cut(s) 265, 2817
EheI GGCGCC 2 cut(s) 265, 2817
ErhI CCWWGG 2 cut(s) 1304, 1483
Esp3I CGTCTC 1 cut(s) 90
FalI AAGNNNNNCTT 8 cut(s) 1444, 1476, 1758, 1790, 2661, 2693, 3303, 3335
FaqI GGGAC 4 cut(s) 22, 1067, 2417, 3400
FauI CCCGC 2 cut(s) 1875, 2350
FauNDI CATATG 3 cut(s) 2206, 2295, 2618
FbaI TGATCA 3 cut(s) 243, 1687, 3295
FblI GTMKAC 1 cut(s) 1222
FokI GGATG 9 cut(s) 503, 761, 1412, 1546, 1735, 2096, 2191, 3143, 3263
FriOI GRGCYC 4 cut(s) 584, 1078, 2770, 3028
FspBI CTAG 6 cut(s) 681, 1305, 2213, 2232, 2771, 3374
GlaI GCGC 5 cut(s) 265, 521, 1896, 2716, 2817
GsaI CCCAGC 1 cut(s) 426
GsuI CTGGAG 2 cut(s) 1638, 3188
HaeII RGCGCY 3 cut(s) 267, 1898, 2819
HaeIII GGCC 6 cut(s) 686, 940, 1405, 1698, 1808, 2480
HapII CCGG 6 cut(s) 59, 267, 485, 687, 941, 3395
HgaI GACGC 2 cut(s) 30, 2711
HhaI GCGC 5 cut(s) 266, 522, 1897, 2717, 2818
Hin1I GRCGYC 5 cut(s) 41, 215, 264, 2816, 3153
Hin6I GCGC 5 cut(s) 264, 520, 1895, 2715, 2816
HinP1I GCGC 5 cut(s) 264, 520, 1895, 2715, 2816
HincII GTYRAC 2 cut(s) 3252, 3392
HindII GTYRAC 2 cut(s) 3252, 3392
HpaI GTTAAC 1 cut(s) 3252
HpaII CCGG 6 cut(s) 59, 267, 485, 687, 941, 3395
HphI GGTGA 9 cut(s) 223, 238, 293, 1737, 1746, 1961, 2341, 2456, 3201
Hpy166II GTNNAC 5 cut(s) 357, 965, 1223, 3252, 3392
Hpy8I GTNNAC 5 cut(s) 357, 965, 1223, 3252, 3392
HpyAV CCTTC 9 cut(s) 295, 524, 1189, 1351, 1416, 2211, 2386, 2463, 2663
HpyCH4IV ACGT 5 cut(s) 215, 315, 1835, 3061, 3153
HpyF3I CTNAG 9 cut(s) 12, 446, 642, 838, 968, 1898, 2341, 2730, 3148
HpySE526I ACGT 5 cut(s) 215, 315, 1835, 3061, 3153
Hsp92I GRCGYC 5 cut(s) 41, 215, 264, 2816, 3153
HspAI GCGC 5 cut(s) 264, 520, 1895, 2715, 2816
KasI GGCGCC 2 cut(s) 263, 2815
KroI GCCGGC 3 cut(s) 266, 686, 940
KroNI GCCGGC 3 cut(s) 268, 688, 942
Ksp22I TGATCA 3 cut(s) 243, 1687, 3295
KspAI GTTAAC 1 cut(s) 3252
LguI GCTCTTC 1 cut(s) 1893
LmnI GCTCC 4 cut(s) 1073, 2203, 3031, 3169
LweI GCATC 6 cut(s) 1379, 1568, 2140, 3128, 3215, 3499
MaeI CTAG 6 cut(s) 681, 1305, 2213, 2232, 2771, 3374
MaeII ACGT 5 cut(s) 215, 315, 1835, 3061, 3153
MaeIII GTNAC 7 cut(s) 211, 1148, 2344, 2558, 2929, 3057, 3459
MfeI CAATTG 3 cut(s) 2283, 2802, 3498
MflI RGATCY 5 cut(s) 1000, 1228, 1457, 2526, 2786
MhlI GDGCHC 6 cut(s) 464, 584, 1078, 2770, 3028, 3368
Mly113I GGCGCC 2 cut(s) 264, 2816
MlyI GAGTC 6 cut(s) 106, 352, 436, 826, 1451, 3389
MmeI TCCRAC 4 cut(s) 577, 1377, 2017, 3096
MreI CGCCGGCG 1 cut(s) 266
MroNI GCCGGC 3 cut(s) 266, 686, 940
MroXI GAANNNNTTC 3 cut(s) 1182, 1773, 2091
MslI CAYNNNNRTG 3 cut(s) 2177, 2445, 3132
MspA1I CMGCKG 5 cut(s) 502, 2066, 2468, 3098, 3472
MspCI CTTAAG 1 cut(s) 983
MspI CCGG 6 cut(s) 59, 267, 485, 687, 941, 3395
MspR9I CCNGG 4 cut(s) 937, 1617, 2820, 3161
MunI CAATTG 3 cut(s) 2283, 2802, 3498
Mva1269I GAATGC 5 cut(s) 437, 562, 653, 1182, 1617
MvaI CCWGG 4 cut(s) 937, 1617, 2820, 3161
MvnI CGCG 2 cut(s) 522, 2890
NaeI GCCGGC 3 cut(s) 268, 688, 942
NarI GGCGCC 2 cut(s) 264, 2816
NdeI CATATG 3 cut(s) 2206, 2295, 2618
NgoMIV GCCGGC 3 cut(s) 266, 686, 940
NlaIV GGNNCC 3 cut(s) 265, 2817, 3027
NmeAIII GCCGAG 2 cut(s) 1828, 3529
NmuCI GTSAC 3 cut(s) 211, 1148, 3459
NspI RCATGY 2 cut(s) 388, 3272
NspV TTCGAA 1 cut(s) 1247
PaeR7I CTCGAG 1 cut(s) 1077
PagI TCATGA 1 cut(s) 3298
PciSI GCTCTTC 1 cut(s) 1893
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 5 cut(s) 437, 562, 653, 1182, 1617
PdiI GCCGGC 3 cut(s) 268, 688, 942
PdmI GAANNNNTTC 3 cut(s) 1182, 1773, 2091
PflFI GACNNNGTC 1 cut(s) 40
PflMI CCANNNNNTGG 1 cut(s) 3166
PleI GAGTC 6 cut(s) 106, 352, 436, 826, 1450, 3388
PluTI GGCGCC 2 cut(s) 267, 2819
PpsI GAGTC 6 cut(s) 106, 352, 436, 826, 1450, 3388
Ppu21I YACGTR 1 cut(s) 3062
Psp124BI GAGCTC 3 cut(s) 584, 1078, 2770
Psp6I CCWGG 4 cut(s) 935, 1615, 2818, 3159
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 4 cut(s) 935, 1615, 2818, 3159
PspN4I GGNNCC 3 cut(s) 265, 2817, 3027
PspPI GGNCC 5 cut(s) 684, 1403, 2478, 2584, 2950
PspXI VCTCGAGB 1 cut(s) 1077
PstI CTGCAG 2 cut(s) 1425, 2005
PstNI CAGNNNCTG 3 cut(s) 445, 3101, 3166
PsuI RGATCY 5 cut(s) 1000, 1228, 1457, 2526, 2786
PsyI GACNNNGTC 1 cut(s) 40
PvuII CAGCTG 5 cut(s) 502, 2066, 2468, 3098, 3472
RsaI GTAC 5 cut(s) 789, 1296, 2451, 2800, 3476
RsaNI GTAC 5 cut(s) 788, 1295, 2450, 2799, 3475
RseI CAYNNNNRTG 3 cut(s) 2177, 2445, 3132
Rsr2I CGGWCCG 1 cut(s) 2584
RsrII CGGWCCG 1 cut(s) 2584
SacI GAGCTC 3 cut(s) 584, 1078, 2770
SapI GCTCTTC 1 cut(s) 1893
Sau96I GGNCC 5 cut(s) 684, 1403, 2478, 2584, 2950
SbfI CCTGCAGG 1 cut(s) 2005
SchI GAGTC 6 cut(s) 106, 352, 436, 826, 1451, 3389
ScrFI CCNGG 4 cut(s) 937, 1617, 2820, 3161
SdaI CCTGCAGG 1 cut(s) 2005
SduI GDGCHC 6 cut(s) 464, 584, 1078, 2770, 3028, 3368
SfaNI GCATC 6 cut(s) 1379, 1568, 2140, 3128, 3215, 3499
SfcI CTRYAG 3 cut(s) 1421, 1728, 2001
SfoI GGCGCC 2 cut(s) 265, 2817
Sfr274I CTCGAG 1 cut(s) 1077
SfuI TTCGAA 1 cut(s) 1247
SgrAI CRCCGGYG 1 cut(s) 266
SinI GGWCC 2 cut(s) 2584, 2950
SlaI CTCGAG 1 cut(s) 1077
SmiMI CAYNNNNRTG 3 cut(s) 2177, 2445, 3132
SmlI CTYRAG 5 cut(s) 983, 1077, 1086, 2505, 3419
SmoI CTYRAG 5 cut(s) 983, 1077, 1086, 2505, 3419
Sse8387I CCTGCAGG 1 cut(s) 2005
SsiI CCGC 9 cut(s) 73, 183, 186, 203, 488, 617, 1757, 1868, 2357
SspDI GGCGCC 2 cut(s) 263, 2815
SspMI CTAG 6 cut(s) 681, 1305, 2213, 2232, 2771, 3374
SstI GAGCTC 3 cut(s) 584, 1078, 2770
StyD4I CCNGG 4 cut(s) 935, 1615, 2818, 3159
StyI CCWWGG 2 cut(s) 1304, 1483
TaiI ACGT 5 cut(s) 218, 318, 1838, 3064, 3156
TaqI TCGA 8 cut(s) 667, 794, 1078, 1193, 1247, 1881, 2390, 3383
TaqII GACCGA 2 cut(s) 2572, 3447
TatI WGTACW 4 cut(s) 787, 1294, 2798, 3474
TauI GCSGC 2 cut(s) 188, 619
TscAI CASTG 8 cut(s) 790, 1159, 1180, 1278, 1294, 2276, 3312, 3366
TseFI GTSAC 3 cut(s) 211, 1148, 3459
Tsp45I GTSAC 3 cut(s) 211, 1148, 3459
TspGWI ACGGA 3 cut(s) 251, 2135, 2751
TspRI CASTG 8 cut(s) 790, 1159, 1180, 1278, 1294, 2276, 3312, 3366
Tth111I GACNNNGTC 1 cut(s) 40
Van91I CCANNNNNTGG 1 cut(s) 3166
Vha464I CTTAAG 1 cut(s) 983
VpaK11BI GGWCC 2 cut(s) 2584, 2950
XapI RAATTY 6 cut(s) 1950, 2029, 2682, 2807, 3217, 3509
XceI RCATGY 2 cut(s) 388, 3272
XhoI CTCGAG 1 cut(s) 1077
XmaJI CCTAGG 1 cut(s) 1304
XmiI GTMKAC 1 cut(s) 1222
XmnI GAANNNNTTC 3 cut(s) 1182, 1773, 2091
XspI CTAG 6 cut(s) 681, 1305, 2213, 2232, 2771, 3374
ZraI GACGTC 2 cut(s) 216, 3154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.