MD06G1127500.v1.1

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003181
Physical Location & Seq
Forward (+)
26944103 .. 26952853
8751 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1127500.v1.1.491

Sequence Viewer

Length: 3573 bp
ATGGCGGCAGCGGCACCACCGTCGTCATCGCCAGTACGGTGGTGGAGGACGGCGTTTCTAACAGTGAGGGACGAAACCCTAACAACTCCTCTGCGCACTTCAATCCCCGAACTCCTCAAACATTCCATCTTCTCCCACTCCCACACTCTCCTCTCCGCCGCCCACGACCTACCTCCGCAGGAAGTCACCTCCGATTTGCTCTTCGTCATGGATCTCGTCATCACAAAGTCTCACGGCGGCGAAGACCTGACTCCTACGTTCACTCACATAACGCACTTGATCCATGATATCTCCCATCGTGTTCCGCTCGAGATCAATTCAGCTTCTTGGACTCTGATCCTCGACGCTTTCAACAAAATGCTTCGCTCGTTCATCGGCTCTTCGTCCTTTACGCCGGTTATGGAAGCTCTGCAGACTCTAAGACGTGTTATGAGCGCGTATCAGAGGAAATGCACGACGGCGGAGGAGGTTCAGCTGGTGAAGTTCCTGCTGCGAATCATTGAGAGCTCTCATGCAGAGTTGAGCTCAGTTCGCAACCAAAGCTCGGTTCTTGAAGTTGGGAAGAGAATGCCGGTGCCTCGATATTGTAGTTTATGGGAAGTTCAGACGCTTGCATTTACTACGCTTGGAGAGGCAATCTCCAGAGTTGGTTTAACCTTGCCGTTGGACATCTGGAGATCAACAATCGAGGTTTTTAGGAAAGTGATGGATGGCTTGGCAGCTAAAAGCCAGCTTGTGGAAGACGCTTTTATGTCCAAGTTTTATTTATCGCTTCTGCATTGTCTGCATTTGACTCTCGCGGATCGTAAGTGTTCATTTTCTGACCATGTGTCAAGTTTTGTGGCTGCTTTGCGAATGTTCTTTTCCTATGGCATTAATAGTAGAACCCAACATACCTGTTCAGCAATTGGTCAAAAGGAAAAGGAACCTAGTTTAGCAAGCCTTAAGTTGGGTTTGGAAGATTCTAAAAAGACCGATCGTACTCCATATAGGCCTCCACACCTGCGCCAAAGGGACAATTCAAATATGAAGAAGAGTGGAGCTCAGGGTTCTCAAAGTTTATCAGATCATGAATCTTCCGCAAATGAGTTTGCATTGTCAGATTCAGATTACAGTGACAGTGATGGATCAATTAAAGACATTGACAACATTCAGAAATCAAAAGTCCGAGTAGCTGCCATTGTTTGTATACAGGATCTTTGTCAAGCTGATTCCAAATCATTTACCAGCCAATGGACGCTGCTATTGCCAACCAGTGATGTACTACAACCTAGGAAATATGAAGCAACACTAATGACATGCTTGCTATTTGATCCTTATCTAAAGGCACGGCTTGCTTCTGCATCAACCCTGGAAGCAATGTTGGATGGCCCGTCATCTGTTTTTCTGCAGGTAGCAGAGTTCAAGGAATCTTCTAAACGTGGATCTTTCACATCACTTTCAAGTTCCCTTGGACATATACTAATGCAGCTTCATACAGGTATTTTATACTTAATTCAACGTGAAACTCATAGTAGACTGATGGCATCCCTGTTCAAGATTCTCATGCTTTTGATATCATCTACACCATATTCAAGAATGCCTGGAGAGTTGTTGCCAACAGTTTTTACGTCTCTACAAGAAAGGATGAAAAATGGATTTCCATTCAAAAGTGATCAGACTGGTCTGCTGGCTTCTTCTATTAGTTGCTTGACAACCGCTCTGAACATCTCACCTTCTTCACTGCAAGTTAAAGACATGCTTCTGGTGGAGATATCTAATGATGAGCTTAATATTGATTTTGTCGATGCTAAAAAGAAGTCAGCTGTTCTCTCTACACTGTTTCAATTTTCAGAACAAGTTAGCAACCCAACGATATGCTTTGAGGCTCTTCTGGCACTTAGGGCTGTGTCCCATAATTACCCAAGCATAATGTTTTCCTGTTGGGAACAAATTTCCACCATTGTTTATGGTGTTTTGAGGGCAGCTATTACTGAAGTTCCTACAGGATATAAGGGCAATACTAGAAATTTTGTTGGATTTATTGGGGAAAAAGTCATTACAGCTGCTATTAAAGTTTTGGACGAATGTCTACGGGCAATATCTGGATTTAAAGGAACTGAAGACCCTTTAGATGATAAATTACTTGATGCTCCATTTATTTCTGACTGCATAAGGATGAAAAAAGTTTCATCTGCCCCTTTTTATGAACCAGAGAACTCAGAAAATACTAGAGATGAACCCACATCATGTCAATCTGGAACAGAGCAGTGGTGTGAGACAATTGAGAAACATATGTCTCTGATTCTTCACCATCCTTCTGCAGTGGTGAGAGCAGCCTCTGTTACATGTTTTGCGGGTATAACTTCTTCTGTATTCTTCTCATTGTCGAAGGAAAAACAAAACTTCATTCTTTCTTCTTCTGTTCGTGCTGCCGTAAGTGATGATGTGCCTTCGGTTAGGTCAGCTGCTTGTCGAGCCATTGGAGTCATATCAATGTTTCCTCAAGTTTCTCAAAGTGCAGAGATCCTTGATAAATTTGTTCATGCTGTGGAGATTAACACTCGTGATCCCCTGATCTCGGTGCGAATAACTGCTTCTTGGGCTCTGGCAAACATATGTGATTCTATCCGTCATTGTATTGATGATTTTGCCTTGAAACAATCTGGAGGCTATCCTGAAATCTCTAAATTATTCACAGTATTAACCGAGTGCGCTTTGCGTCTGACTAAGGATGGAGATAAGATTAAATCAAATGCTGTAAGGGCTCTAGGGAATCTTTCAAGATCTATCAAATATAGAAGCAATAGTGACAGGATAGTAGACAATAATGGCATGCCAATAAAGAGCACTAAACCTGACAAAATATCATCAAGCAATTACAGGGAAGGAAGTCAAAGAGATGTATCAATATCATGCCATCCAGCTTCTTTGGGAGATTCTCATTGGCTAGAGAGGGTCGTTCAGGCATTTATTTCTTGTGTTACGACTGGTAATGTTAAGGTCCAATGGAATGTTTGTCATGCTTTAAGCAACCTATTTCTGAATGAGACATTAAGACTGCGAGAGATGGATTGGGCTTCGTCTGTGTTTAGTATTCTTTTACTGCTGTTACGCGATTCTTCCAACTTTAAGATCAGGATTCAAGCTGCTTCTGCTTTGGCCGTGCCAGCATCAGTATTTGATTATGGAGAATCCTTCTCCGATGTCATCCAGGGTCTGGTGCATATATTAGAGAATCAAGGTTCAGATCGGATTGCATCACCGTCAAATTTCAAATACAGGGTTGCACTTGAAAACCAGTTGACCTCAACCGTGTTGCATGTTCTTATCCTCACTTCAAGTTCGGATCATGAACCCGTCAAAGATTTCCTTGTAAAAAAAGCCTCGTTCCTCGAAGACTGGTTCAAGGCGCTATGCTCGTCACTTGGAGAGACAAGTTGTCAAGCTGAACTTGAGAATAATAAATCTACCGGGAATCCGAAGAACGAGATGATATGCAACGCAATTGGGTCGCTAATCCAATTGTACAATAGCAGGAAGCATCATGCAATTGCCCAGAAATTTGAGAAGTTAGTGAACGGCATCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4042 HEAT

Protein Analysis

1191

Amino Acids

131.93

Weight (kDa)

7.35

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4042 PF13251 388 - 570 1.7e-52 Domain of unknown function (DUF4042)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1011
Acc16I TGCGCA 1 cut(s) 95
Acc36I ACCTGC 2 cut(s) 1011, 1381
AccB1I GGYRCC 2 cut(s) 13, 574
AccB7I CCANNNNNTGG 3 cut(s) 736, 1233, 3199
AccBSI CCGCTC 2 cut(s) 307, 1700
AccI GTMKAC 4 cut(s) 1189, 1516, 2071, 2802
AccII CGCG 3 cut(s) 437, 800, 3096
AcoI YGGCCR 1 cut(s) 3141
AcsI RAATTY 5 cut(s) 1932, 2008, 2516, 3250, 3542
AcuI CTGAAG 2 cut(s) 1995, 2121
AfaI GTAC 4 cut(s) 36, 982, 1263, 3509
AfiI CCNNNNNNNGG 6 cut(s) 544, 736, 949, 1233, 2560, 3199
AflII CTTAAG 1 cut(s) 944
AflIII ACRYGT 2 cut(s) 424, 2327
AhdI GACNNNNNGTC 2 cut(s) 829, 3420
AjiI CACGTC 1 cut(s) 425
AjnI CCWGG 3 cut(s) 1350, 1582, 3192
AjuI GAANNNNNNNTTGG 2 cut(s) 531, 563
Alw21I GWGCWC 4 cut(s) 509, 527, 1045, 2831
Alw26I GTCTC 6 cut(s) 234, 1617, 2252, 2283, 3023, 3407
AlwNI CAGNNNCTG 2 cut(s) 2321, 3199
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 3 cut(s) 992, 1369, 3141
ApoI RAATTY 5 cut(s) 1932, 2008, 2516, 3250, 3542
AseI ATTAAT 1 cut(s) 876
AspA2I CCTAGG 1 cut(s) 1271
AspLEI GCGC 5 cut(s) 96, 437, 1008, 2696, 3394
AspS9I GGNCC 2 cut(s) 1370, 2983
AsuC2I CCSGG 1 cut(s) 3454
AsuHPI GGTGA 6 cut(s) 178, 490, 1704, 2282, 2320, 3234
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 1 cut(s) 2983
AvrII CCTAGG 1 cut(s) 1271
BanI GGYRCC 2 cut(s) 13, 574
BanII GRGCYC 5 cut(s) 509, 527, 1045, 2587, 2749
BarI GAAGNNNNNNTAC 2 cut(s) 2332, 2364
BauI CACGAG 1 cut(s) 2544
BbsI GAAGAC 4 cut(s) 249, 747, 2109, 3384
Bbv12I GWGCWC 4 cut(s) 509, 527, 1045, 2831
BceAI ACGGC 7 cut(s) 66, 250, 474, 646, 1346, 2399, 3128
BcgI CGANNNNNNTGC 3 cut(s) 37, 3474, 3508
BciT130I CCWGG 3 cut(s) 1352, 1584, 3194
BclI TGATCA 1 cut(s) 1654
BcnI CCSGG 1 cut(s) 3454
BcoDI GTCTC 6 cut(s) 234, 1617, 2252, 2283, 3023, 3407
BfaI CTAG 6 cut(s) 930, 1272, 2004, 2211, 2750, 2930
BfmI CTRYAG 4 cut(s) 410, 1388, 1983, 2301
BfoI RGCGCY 1 cut(s) 3395
BfrI CTTAAG 1 cut(s) 944
BfuAI ACCTGC 2 cut(s) 1011, 1381
BglII AGATCT 1 cut(s) 2765
BlnI CCTAGG 1 cut(s) 1271
Bme1390I CCNGG 4 cut(s) 1352, 1584, 3194, 3454
Bme18I GGWCC 1 cut(s) 2983
BmeRI GACNNNNNGTC 2 cut(s) 829, 3420
BmeT110I CYCGRG 1 cut(s) 308
BmgBI CACGTC 1 cut(s) 425
BmgT120I GGNCC 2 cut(s) 1370, 2983
BmiI GGNNCC 3 cut(s) 15, 576, 927
BmrFI CCNGG 4 cut(s) 1352, 1584, 3194, 3454
BmsI GCATC 7 cut(s) 1352, 1535, 1777, 2119, 3161, 3248, 3532
BoxI GACNNNNGTC 1 cut(s) 2067
BpiI GAAGAC 4 cut(s) 249, 747, 2109, 3384
BplI GAGNNNNNCTC 6 cut(s) 509, 541, 623, 655, 1027, 1059
BpmI CTGGAG 4 cut(s) 625, 694, 1605, 2667
Bpu10I CCTNAGC 1 cut(s) 1044
BpuEI CTTGAG 2 cut(s) 2469, 3455
BpuMI CCSGG 1 cut(s) 3454
BsaBI GATNNNNATC 1 cut(s) 1317
BsaJI CCNNGG 4 cut(s) 1271, 1350, 1450, 3193
Bsc4I CCNNNNNNNGG 6 cut(s) 544, 736, 949, 1233, 2560, 3199
Bse118I RCCGGY 2 cut(s) 394, 571
Bse1I ACTGG 7 cut(s) 32, 1254, 1666, 2974, 3280, 3386, 3568
Bse3DI GCAATG 1 cut(s) 1365
Bse8I GATNNNNATC 1 cut(s) 1317
BseBI CCWGG 3 cut(s) 1352, 1584, 3194
BseDI CCNNGG 4 cut(s) 1271, 1350, 1450, 3193
BseJI GATNNNNATC 1 cut(s) 1317
BseLI CCNNNNNNNGG 6 cut(s) 544, 736, 949, 1233, 2560, 3199
BseMI GCAATG 1 cut(s) 1365
BseMII CTCAG 3 cut(s) 540, 1058, 2214
BseNI ACTGG 7 cut(s) 32, 1254, 1666, 2974, 3280, 3386, 3568
BseRI GAGGAG 4 cut(s) 78, 104, 140, 479
BsgI GTGCAG 1 cut(s) 2520
Bsh1236I CGCG 3 cut(s) 437, 800, 3096
Bsh1285I CGRYCG 1 cut(s) 979
BshFI GGCC 3 cut(s) 994, 1371, 3143
BshNI GGYRCC 2 cut(s) 13, 574
BsiEI CGRYCG 1 cut(s) 979
BsiHKAI GWGCWC 4 cut(s) 509, 527, 1045, 2831
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 3 cut(s) 395, 572, 3453
BslFI GGGAC 3 cut(s) 83, 1028, 1876
BslI CCNNNNNNNGG 6 cut(s) 544, 736, 949, 1233, 2560, 3199
BsmAI GTCTC 6 cut(s) 234, 1617, 2252, 2283, 3023, 3407
BsmBI CGTCTC 1 cut(s) 1617
BsmFI GGGAC 3 cut(s) 83, 1028, 1876
BsmI GAATGC 2 cut(s) 573, 1584
BsnI GGCC 3 cut(s) 994, 1371, 3143
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 6 cut(s) 509, 527, 1045, 2587, 2749, 2831
Bsp1407I TGTACA 1 cut(s) 3507
BspANI GGCC 3 cut(s) 994, 1371, 3143
BspCNI CTCAG 3 cut(s) 539, 1057, 2213
BspFNI CGCG 3 cut(s) 437, 800, 3096
BspHI TCATGA 2 cut(s) 1069, 3331
BspLI GGNNCC 3 cut(s) 15, 576, 927
BspMAI CTGCAG 3 cut(s) 414, 1392, 2305
BspMI ACCTGC 2 cut(s) 1011, 1381
BspQI GCTCTTC 3 cut(s) 206, 385, 1875
BspT107I GGYRCC 2 cut(s) 13, 574
BspTI CTTAAG 1 cut(s) 944
BsrBI CCGCTC 2 cut(s) 307, 1700
BsrDI GCAATG 1 cut(s) 1365
BsrFI RCCGGY 2 cut(s) 394, 571
BsrGI TGTACA 1 cut(s) 3507
BsrI ACTGG 7 cut(s) 32, 1254, 1666, 2974, 3280, 3386, 3568
BssAI RCCGGY 2 cut(s) 394, 571
BssECI CCNNGG 4 cut(s) 1271, 1350, 1450, 3193
BssNAI GTATAC 1 cut(s) 1190
BssSI CACGAG 1 cut(s) 2544
BssT1I CCWWGG 2 cut(s) 1271, 1450
Bst1107I GTATAC 1 cut(s) 1190
Bst2BI CACGAG 1 cut(s) 2544
Bst2UI CCWGG 3 cut(s) 1352, 1584, 3194
Bst6I CTCTTC 5 cut(s) 206, 385, 557, 1028, 1875
BstAFI CTTAAG 1 cut(s) 944
BstAPI GCANNNNNTGC 2 cut(s) 784, 1334
BstAUI TGTACA 1 cut(s) 3507
BstC8I GCNNGC 8 cut(s) 612, 731, 940, 1304, 1335, 1671, 2816, 3150
BstDEI CTNAG 6 cut(s) 419, 526, 1044, 1880, 2200, 2709
BstFNI CGCG 3 cut(s) 437, 800, 3096
BstH2I RGCGCY 1 cut(s) 3395
BstHHI GCGC 5 cut(s) 96, 437, 1008, 2696, 3394
BstMAI GTCTC 6 cut(s) 234, 1617, 2252, 2283, 3023, 3407
BstMCI CGRYCG 1 cut(s) 979
BstNI CCWGG 3 cut(s) 1352, 1584, 3194
BstNSI RCATGY 5 cut(s) 1302, 1741, 2331, 2818, 3305
BstPAI GACNNNNGTC 1 cut(s) 2067
BstSCI CCNGG 4 cut(s) 1350, 1582, 3192, 3452
BstSFI CTRYAG 4 cut(s) 410, 1388, 1983, 2301
BstUI CGCG 3 cut(s) 437, 800, 3096
BstV2I GAAGAC 4 cut(s) 249, 747, 2109, 3384
BstX2I RGATCY 5 cut(s) 211, 1195, 1424, 2505, 2765
BstXI CCANNNNNNTGG 1 cut(s) 39
BstYI RGATCY 5 cut(s) 211, 1195, 1424, 2505, 2765
BstZ17I GTATAC 1 cut(s) 1190
BsuRI GGCC 3 cut(s) 994, 1371, 3143
BtgZI GCGATG 1 cut(s) 12
BtrI CACGTC 1 cut(s) 425
BtsI GCAGTG 3 cut(s) 1721, 2255, 2310
BtsIMutI CAGTG 8 cut(s) 69, 1120, 1126, 1261, 1721, 1817, 2255, 2310
BveI ACCTGC 2 cut(s) 1011, 1381
Cac8I GCNNGC 8 cut(s) 612, 731, 940, 1304, 1335, 1671, 2816, 3150
CaiI CAGNNNCTG 2 cut(s) 2321, 3199
CciI TCATGA 2 cut(s) 1069, 3331
CfoI GCGC 5 cut(s) 96, 437, 1008, 2696, 3394
Cfr10I RCCGGY 2 cut(s) 394, 571
Cfr13I GGNCC 2 cut(s) 1370, 2983
CseI GACGC 5 cut(s) 353, 616, 752, 1246, 2690
Csp6I GTAC 4 cut(s) 35, 981, 1262, 3508
CspCI CAANNNNNGTGG 2 cut(s) 822, 857
CviQI GTAC 4 cut(s) 35, 981, 1262, 3508
DdeI CTNAG 6 cut(s) 419, 526, 1044, 1880, 2200, 2709
DraI TTTAAA 1 cut(s) 2092
DriI GACNNNNNGTC 2 cut(s) 829, 3420
EaeI YGGCCR 1 cut(s) 3141
Eam1104I CTCTTC 5 cut(s) 206, 385, 557, 1028, 1875
Eam1105I GACNNNNNGTC 2 cut(s) 829, 3420
EarI CTCTTC 5 cut(s) 206, 385, 557, 1028, 1875
EciI GGCGGA 2 cut(s) 145, 476
Ecl136II GAGCTC 3 cut(s) 507, 525, 1043
Eco130I CCWWGG 2 cut(s) 1271, 1450
Eco147I AGGCCT 1 cut(s) 994
Eco24I GRGCYC 5 cut(s) 509, 527, 1045, 2587, 2749
Eco32I GATATC 3 cut(s) 289, 1557, 1755
Eco47I GGWCC 1 cut(s) 2983
Eco53kI GAGCTC 3 cut(s) 507, 525, 1043
Eco57I CTGAAG 2 cut(s) 1995, 2121
Eco88I CYCGRG 1 cut(s) 308
EcoICRI GAGCTC 3 cut(s) 507, 525, 1043
EcoRII CCWGG 3 cut(s) 1350, 1582, 3192
EcoRV GATATC 3 cut(s) 289, 1557, 1755
EcoT14I CCWWGG 2 cut(s) 1271, 1450
EcoT38I GRGCYC 5 cut(s) 509, 527, 1045, 2587, 2749
ErhI CCWWGG 2 cut(s) 1271, 1450
Esp3I CGTCTC 1 cut(s) 1617
FalI AAGNNNNNCTT 6 cut(s) 1725, 1757, 3336, 3368, 3417, 3449
FaqI GGGAC 3 cut(s) 83, 1028, 1876
FauI CCCGC 1 cut(s) 2329
FauNDI CATATG 2 cut(s) 2274, 2597
FbaI TGATCA 1 cut(s) 1654
FblI GTMKAC 4 cut(s) 1189, 1516, 2071, 2802
FriOI GRGCYC 5 cut(s) 509, 527, 1045, 2587, 2749
FspBI CTAG 6 cut(s) 930, 1272, 2004, 2211, 2750, 2930
FspI TGCGCA 1 cut(s) 95
GlaI GCGC 5 cut(s) 95, 436, 1007, 2695, 3393
GsuI CTGGAG 4 cut(s) 625, 694, 1605, 2667
HaeII RGCGCY 1 cut(s) 3395
HaeIII GGCC 3 cut(s) 994, 1371, 3143
HapII CCGG 3 cut(s) 395, 572, 3453
HgaI GACGC 5 cut(s) 353, 616, 752, 1246, 2690
HhaI GCGC 5 cut(s) 96, 437, 1008, 2696, 3394
Hin6I GCGC 5 cut(s) 94, 435, 1006, 2694, 3392
HinP1I GCGC 5 cut(s) 94, 435, 1006, 2694, 3392
HincII GTYRAC 1 cut(s) 3285
HindII GTYRAC 1 cut(s) 3285
HpaII CCGG 3 cut(s) 395, 572, 3453
HphI GGTGA 6 cut(s) 178, 490, 1704, 2282, 2320, 3234
Hpy166II GTNNAC 7 cut(s) 261, 1190, 1517, 2072, 2803, 3285, 3559
Hpy8I GTNNAC 7 cut(s) 261, 1190, 1517, 2072, 2803, 3285, 3559
Hpy99I CGWCG 3 cut(s) 25, 347, 460
HpyAV CCTTC 6 cut(s) 1725, 2307, 2365, 2442, 2861, 3187
HpyCH4IV ACGT 5 cut(s) 257, 424, 1420, 1501, 1610
HpyF3I CTNAG 6 cut(s) 419, 526, 1044, 1880, 2200, 2709
HpySE526I ACGT 5 cut(s) 257, 424, 1420, 1501, 1610
HspAI GCGC 5 cut(s) 94, 435, 1006, 2694, 3392
Ksp22I TGATCA 1 cut(s) 1654
LguI GCTCTTC 3 cut(s) 206, 385, 1875
LmnI GCTCC 2 cut(s) 1040, 2137
LweI GCATC 7 cut(s) 1352, 1535, 1777, 2119, 3161, 3248, 3532
MaeI CTAG 6 cut(s) 930, 1272, 2004, 2211, 2750, 2930
MaeII ACGT 5 cut(s) 257, 424, 1420, 1501, 1610
MaeIII GTNAC 7 cut(s) 184, 1115, 2323, 2789, 2962, 3090, 3402
MbiI CCGCTC 2 cut(s) 307, 1700
MfeI CAATTG 5 cut(s) 906, 2262, 3486, 3503, 3531
MflI RGATCY 5 cut(s) 211, 1195, 1424, 2505, 2765
MhlI GDGCHC 6 cut(s) 509, 527, 1045, 2587, 2749, 2831
MlyI GAGTC 5 cut(s) 244, 325, 409, 787, 2475
MmeI TCCRAC 4 cut(s) 645, 1344, 1996, 3129
MslI CAYNNNNRTG 2 cut(s) 2156, 2474
MspA1I CMGCKG 5 cut(s) 11, 475, 1805, 2045, 2447
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 3 cut(s) 395, 572, 3453
MspR9I CCNGG 4 cut(s) 1352, 1584, 3194, 3454
MunI CAATTG 5 cut(s) 906, 2262, 3486, 3503, 3531
Mva1269I GAATGC 2 cut(s) 573, 1584
MvaI CCWGG 3 cut(s) 1352, 1584, 3194
MvnI CGCG 3 cut(s) 437, 800, 3096
NciI CCSGG 1 cut(s) 3454
NdeI CATATG 2 cut(s) 2274, 2597
NlaIV GGNNCC 3 cut(s) 15, 576, 927
NmuCI GTSAC 4 cut(s) 184, 1115, 2789, 3402
NsbI TGCGCA 1 cut(s) 95
NspI RCATGY 5 cut(s) 1302, 1741, 2331, 2818, 3305
PaeI GCATGC 1 cut(s) 2818
PaeR7I CTCGAG 1 cut(s) 308
PagI TCATGA 2 cut(s) 1069, 3331
PaqCI CACCTGC 1 cut(s) 1011
PceI AGGCCT 1 cut(s) 994
PciI ACATGT 1 cut(s) 2327
PciSI GCTCTTC 3 cut(s) 206, 385, 1875
PctI GAATGC 2 cut(s) 573, 1584
PflMI CCANNNNNTGG 3 cut(s) 736, 1233, 3199
Ple19I CGATCG 1 cut(s) 979
PleI GAGTC 5 cut(s) 244, 325, 409, 787, 2474
PpsI GAGTC 5 cut(s) 244, 325, 409, 787, 2474
PscI ACATGT 1 cut(s) 2327
PshAI GACNNNNGTC 1 cut(s) 2067
PshBI ATTAAT 1 cut(s) 876
Psp124BI GAGCTC 3 cut(s) 509, 527, 1045
Psp6I CCWGG 3 cut(s) 1350, 1582, 3192
PspGI CCWGG 3 cut(s) 1350, 1582, 3192
PspN4I GGNNCC 3 cut(s) 15, 576, 927
PspPI GGNCC 2 cut(s) 1370, 2983
PsrI GAACNNNNNNTAC 2 cut(s) 877, 909
PstI CTGCAG 3 cut(s) 414, 1392, 2305
PstNI CAGNNNCTG 2 cut(s) 2321, 3199
PsuI RGATCY 5 cut(s) 211, 1195, 1424, 2505, 2765
PvuI CGATCG 1 cut(s) 979
PvuII CAGCTG 4 cut(s) 475, 1805, 2045, 2447
RsaI GTAC 4 cut(s) 36, 982, 1263, 3509
RsaNI GTAC 4 cut(s) 35, 981, 1262, 3508
RseI CAYNNNNRTG 2 cut(s) 2156, 2474
SacI GAGCTC 3 cut(s) 509, 527, 1045
SapI GCTCTTC 3 cut(s) 206, 385, 1875
Sau96I GGNCC 2 cut(s) 1370, 2983
SchI GAGTC 5 cut(s) 244, 325, 409, 787, 2475
ScrFI CCNGG 4 cut(s) 1352, 1584, 3194, 3454
SduI GDGCHC 6 cut(s) 509, 527, 1045, 2587, 2749, 2831
SfaNI GCATC 7 cut(s) 1352, 1535, 1777, 2119, 3161, 3248, 3532
SfcI CTRYAG 4 cut(s) 410, 1388, 1983, 2301
Sfr274I CTCGAG 1 cut(s) 308
SinI GGWCC 1 cut(s) 2983
SlaI CTCGAG 1 cut(s) 308
SmiMI CAYNNNNRTG 2 cut(s) 2156, 2474
SmlI CTYRAG 4 cut(s) 308, 944, 2484, 3434
SmoI CTYRAG 4 cut(s) 308, 944, 2484, 3434
SphI GCATGC 1 cut(s) 2818
SseBI AGGCCT 1 cut(s) 994
SspI AATATT 1 cut(s) 1774
SspMI CTAG 6 cut(s) 930, 1272, 2004, 2211, 2750, 2930
SstI GAGCTC 3 cut(s) 509, 527, 1045
StuI AGGCCT 1 cut(s) 994
StyD4I CCNGG 4 cut(s) 1350, 1582, 3192, 3452
StyI CCWWGG 2 cut(s) 1271, 1450
TaiI ACGT 5 cut(s) 260, 427, 1423, 1504, 1613
TaqI TCGA 8 cut(s) 309, 342, 580, 687, 1785, 2369, 2455, 3375
TaqII GACCGA 1 cut(s) 989
TatI WGTACW 2 cut(s) 1261, 3507
TauI GCSGC 4 cut(s) 8, 14, 161, 240
TscAI CASTG 8 cut(s) 69, 1120, 1126, 1261, 1728, 1824, 2255, 2310
TseFI GTSAC 4 cut(s) 184, 1115, 2789, 3402
Tsp45I GTSAC 4 cut(s) 184, 1115, 2789, 3402
TspGWI ACGGA 1 cut(s) 2600
TspRI CASTG 8 cut(s) 69, 1120, 1126, 1261, 1728, 1824, 2255, 2310
Van91I CCANNNNNTGG 3 cut(s) 736, 1233, 3199
Vha464I CTTAAG 1 cut(s) 944
VpaK11BI GGWCC 1 cut(s) 2983
VspI ATTAAT 1 cut(s) 876
XapI RAATTY 5 cut(s) 1932, 2008, 2516, 3250, 3542
XceI RCATGY 5 cut(s) 1302, 1741, 2331, 2818, 3305
XcmI CCANNNNNNNNNTGG 1 cut(s) 39
XhoI CTCGAG 1 cut(s) 308
XmaJI CCTAGG 1 cut(s) 1271
XmiI GTMKAC 4 cut(s) 1189, 1516, 2071, 2802
XspI CTAG 6 cut(s) 930, 1272, 2004, 2211, 2750, 2930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.