FvH4_5g13660

SPIRAL1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003119
Physical Location & Seq
Reverse (-)
7725624 .. 7727688
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13660.t1

Sequence Viewer

Length: 342 bp
ATGCGTCGTGGAGGGAGTAGTGGAGGGGGAGGCAGTTCTTTGGGCTATCTTTTTGGAGGTGGAGAAGAAGGTCAGAATCCTGCCCCCAAAAAGGTTGTAACTCCCAAGGTTCAGGTGGAAGTTGAACCTACTCCTAAGCCTACTGCTGCTGCACCACCACCTGCGGAAGTGATCAATAAGGAGACTCCAGCAGGTGTTCCTGGGAACACTTCAAACAACTACTTTAGAGCTGATGGCCAGAACCGTGGAAACTTCCTCACAGATCGTCCATCCACAAAGGTCCATGCAGCTCCTGGTGGGGGTTCATCTCTCGATTACCTGTTTGGAGGTGGTGGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

11.38

Weight (kDa)

9.1

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 169, 182
Acc36I ACCTGC 2 cut(s) 169, 182
AciI CCGC 1 cut(s) 164
AcoI YGGCCR 2 cut(s) 235, 334
AfiI CCNNNNNNNGG 2 cut(s) 91, 299
AgsI TTSAA 2 cut(s) 125, 213
AjnI CCWGG 2 cut(s) 199, 292
AluBI AGCT 2 cut(s) 230, 290
AluI AGCT 2 cut(s) 230, 290
Alw26I GTCTC 1 cut(s) 176
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 2 cut(s) 235, 334
ApeKI GCWGC 3 cut(s) 146, 149, 287
AspS9I GGNCC 1 cut(s) 280
AvaII GGWCC 1 cut(s) 280
BalI TGGCCA 2 cut(s) 237, 336
BbvI GCAGC 3 cut(s) 133, 136, 299
BccI CCATC 2 cut(s) 227, 277
BciT130I CCWGG 2 cut(s) 201, 294
BclI TGATCA 1 cut(s) 171
BcoDI GTCTC 1 cut(s) 176
BfuAI ACCTGC 2 cut(s) 169, 182
BisI GCNGC 3 cut(s) 147, 150, 288
BlsI GCNGC 3 cut(s) 148, 151, 289
Bme1390I CCNGG 2 cut(s) 201, 294
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 280
BmrFI CCNGG 2 cut(s) 201, 294
BpmI CTGGAG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 135
BsaJI CCNNGG 3 cut(s) 105, 200, 244
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 299
Bse1I ACTGG 1 cut(s) 337
BseBI CCWGG 2 cut(s) 201, 294
BseDI CCNNGG 3 cut(s) 105, 200, 244
BseGI GGATG 1 cut(s) 269
BseLI CCNNNNNNNGG 2 cut(s) 91, 299
BseNI ACTGG 1 cut(s) 337
BseXI GCAGC 3 cut(s) 133, 136, 299
BsgI GTGCAG 1 cut(s) 135
BshFI GGCC 2 cut(s) 237, 336
BslI CCNNNNNNNGG 2 cut(s) 91, 299
BsmAI GTCTC 1 cut(s) 176
BsnI GGCC 2 cut(s) 237, 336
Bsp143I GATC 2 cut(s) 171, 262
BspACI CCGC 1 cut(s) 164
BspANI GGCC 2 cut(s) 237, 336
BspMI ACCTGC 2 cut(s) 169, 182
BsrI ACTGG 1 cut(s) 337
BssECI CCNNGG 3 cut(s) 105, 200, 244
BssMI GATC 2 cut(s) 171, 262
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 2 cut(s) 201, 294
Bst4CI ACNGT 1 cut(s) 245
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 1 cut(s) 269
BstKTI GATC 2 cut(s) 174, 265
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 2 cut(s) 171, 262
BstNI CCWGG 2 cut(s) 201, 294
BstSCI CCNGG 2 cut(s) 199, 292
BstV1I GCAGC 3 cut(s) 133, 136, 299
BstXI CCANNNNNNTGG 1 cut(s) 245
BsuRI GGCC 2 cut(s) 237, 336
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 1 cut(s) 269
BveI ACCTGC 2 cut(s) 169, 182
CaiI CAGNNNCTG 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 280
CviAII CATG 1 cut(s) 284
CviJI RGCY 6 cut(s) 45, 139, 230, 237, 290, 336
CviKI_1 RGCY 6 cut(s) 45, 139, 230, 237, 290, 336
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 173, 264
DpnII GATC 2 cut(s) 171, 262
EaeI YGGCCR 2 cut(s) 235, 334
Eco130I CCWWGG 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 280
EcoRII CCWGG 2 cut(s) 199, 292
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 1 cut(s) 287
FaiI YATR 1 cut(s) 285
FatI CATG 1 cut(s) 283
FbaI TGATCA 1 cut(s) 171
Fnu4HI GCNGC 3 cut(s) 147, 150, 288
FokI GGATG 1 cut(s) 256
Fsp4HI GCNGC 3 cut(s) 147, 150, 288
GluI GCNGC 3 cut(s) 147, 150, 288
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 2 cut(s) 237, 336
Hin1II CATG 1 cut(s) 287
HinfI GANTC 2 cut(s) 76, 184
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 1 cut(s) 311
Hpy99I CGWCG 1 cut(s) 9
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4V TGCA 2 cut(s) 152, 287
HpyF3I CTNAG 1 cut(s) 135
Hsp92II CATG 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 2 cut(s) 171, 262
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 3 cut(s) 133, 136, 299
MaeIII GTNAC 1 cut(s) 97
MalI GATC 2 cut(s) 173, 264
MboI GATC 2 cut(s) 171, 262
MboII GAAGA 1 cut(s) 77
MlsI TGGCCA 2 cut(s) 237, 336
MluNI TGGCCA 2 cut(s) 237, 336
MlyI GAGTC 1 cut(s) 178
MnlI CCTC 6 cut(s) 5, 17, 23, 50, 266, 320
Mox20I TGGCCA 2 cut(s) 237, 336
MscI TGGCCA 2 cut(s) 237, 336
Msp20I TGGCCA 2 cut(s) 237, 336
MspR9I CCNGG 2 cut(s) 201, 294
MvaI CCWGG 2 cut(s) 201, 294
NdeII GATC 2 cut(s) 171, 262
NlaIII CATG 1 cut(s) 287
PaqCI CACCTGC 2 cut(s) 169, 182
PfeI GAWTC 1 cut(s) 76
PkrI GCNGC 3 cut(s) 148, 151, 289
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
Psp6I CCWGG 2 cut(s) 199, 292
PspGI CCWGG 2 cut(s) 199, 292
PspPI GGNCC 1 cut(s) 280
PstNI CAGNNNCTG 1 cut(s) 293
SatI GCNGC 3 cut(s) 147, 150, 288
Sau3AI GATC 2 cut(s) 171, 262
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 2 cut(s) 201, 294
SinI GGWCC 1 cut(s) 280
SsiI CCGC 1 cut(s) 164
StyD4I CCNGG 2 cut(s) 199, 292
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 245
TaqI TCGA 1 cut(s) 312
TfiI GAWTC 1 cut(s) 76
TseI GCWGC 3 cut(s) 146, 149, 287
TspDTI ATGAA 1 cut(s) 294
VpaK11BI GGWCC 1 cut(s) 280
XcmI CCANNNNNNNNNTGG 2 cut(s) 112, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.